Igh

Summary

Gene Symbol: Igh
Description: immunoglobulin heavy chain complex
Species: mouse

Top Publications

  1. pmc Elucidation of IgH intronic enhancer functions via germ-line deletion
    Thomas Perlot
    The CBR Institute for Biomedical Research, Inc, and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:14362-7. 2005
  2. pmc CTCF-binding elements mediate control of V(D)J recombination
    Chunguang Guo
    Howard Hughes Medical Institute, The Children s Hospital, The Immune Disease Institute, Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 477:424-30. 2011
  3. doi Replacement of Imu-Cmu intron by NeoR gene alters Imu germ-line expression but has no effect on V(D)J recombination
    Dania Haddad
    CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Equipe Instabilité génétique et régulation transcriptionnelle, 205 route de Narbonne, 31077 Toulouse, France
    Mol Immunol 47:961-71. 2010
  4. pmc Initial clonal expansion of germinal center B cells takes place at the perimeter of follicles
    Francis Coffey
    Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Immunity 30:599-609. 2009
  5. ncbi IgH class switching and translocations use a robust non-classical end-joining pathway
    Catherine T Yan
    Howard Hughes Medical Institute, of Genetics, Boston, Massachusetts 02115, USA
    Nature 449:478-82. 2007
  6. ncbi Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation
    Nicole M Haynes
    Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    J Immunol 179:5099-108. 2007
  7. pmc Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene
    Martin Fuxa
    Research Institute of Molecular Pathology, Vienna Biocente, A 1030 Vienna, Austria
    Genes Dev 18:411-22. 2004
  8. ncbi Imaging of germinal center selection events during affinity maturation
    Christopher D C Allen
    Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, USA
    Science 315:528-31. 2007
  9. ncbi Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice
    Sung Sup Park
    Laboratory of Genetics, Genetics Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Cancer Res 65:1306-15. 2005
  10. pmc Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4
    Cristian Boboila
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 207:417-27. 2010

Scientific Experts

Detail Information

Publications131 found, 100 shown here

  1. pmc Elucidation of IgH intronic enhancer functions via germ-line deletion
    Thomas Perlot
    The CBR Institute for Biomedical Research, Inc, and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:14362-7. 2005
    Studies of chimeric mice demonstrated that the core Ig heavy chain (IgH) intronic enhancer (iEmu) functions in V(D)J and class switch recombination at the IgH locus...
  2. pmc CTCF-binding elements mediate control of V(D)J recombination
    Chunguang Guo
    Howard Hughes Medical Institute, The Children s Hospital, The Immune Disease Institute, Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 477:424-30. 2011
    Immunoglobulin heavy chain (IgH) variable region exons are assembled from V(H), D and J(H) gene segments in developing B lymphocytes. Within the 2...
  3. doi Replacement of Imu-Cmu intron by NeoR gene alters Imu germ-line expression but has no effect on V(D)J recombination
    Dania Haddad
    CNRS UMR 5089, Institut de Pharmacologie et de Biologie Structurale, Equipe Instabilité génétique et régulation transcriptionnelle, 205 route de Narbonne, 31077 Toulouse, France
    Mol Immunol 47:961-71. 2010
    ..and enhancers during V(D)J assembly and class switch recombination (CSR) in the immunoglobulin heavy chain (IgH) locus...
  4. pmc Initial clonal expansion of germinal center B cells takes place at the perimeter of follicles
    Francis Coffey
    Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Immunity 30:599-609. 2009
    ..The expanded clones were subsequently observed in the center of follicles, suggesting that GCs are created by coalescence of B cells from this follicular perimeter response...
  5. ncbi IgH class switching and translocations use a robust non-classical end-joining pathway
    Catherine T Yan
    Howard Hughes Medical Institute, of Genetics, Boston, Massachusetts 02115, USA
    Nature 449:478-82. 2007
    ..C-NHEJ indeed catalyses CSR joins, because C-NHEJ-deficient B cells had decreased CSR and substantial levels of IgH locus (immunoglobulin heavy chain, encoded by Igh) chromosomal breaks...
  6. ncbi Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation
    Nicole M Haynes
    Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    J Immunol 179:5099-108. 2007
    ....
  7. pmc Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene
    Martin Fuxa
    Research Institute of Molecular Pathology, Vienna Biocente, A 1030 Vienna, Austria
    Genes Dev 18:411-22. 2004
    The subnuclear location and chromatin state of the immunoglobulin heavy-chain (IgH) locus have been implicated in the control of VDJ recombination...
  8. ncbi Imaging of germinal center selection events during affinity maturation
    Christopher D C Allen
    Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, USA
    Science 315:528-31. 2007
    ..On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated...
  9. ncbi Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice
    Sung Sup Park
    Laboratory of Genetics, Genetics Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Cancer Res 65:1306-15. 2005
    ..to insert a His(6)-tagged mouse c-Myc cDNA, Myc(His), head to head into the mouse immunoglobulin heavy-chain locus, Igh, just 5' of the intronic enhancer, Emu...
  10. pmc Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4
    Cristian Boboila
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 207:417-27. 2010
    ..During IgH class switch recombination (CSR) in B lymphocytes, switch (S) region DSBs are joined by C-NHEJ to form junctions ..
  11. ncbi Growth retardation and leaky SCID phenotype of Ku70-deficient mice
    Y Gu
    The Howard Hughes Medical Institute, Children s Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Immunity 7:653-65. 1997
    ..These unanticipated features of the Ku70-deficient phenotype with respect to lymphocyte development and V(D)J recombination may reflect differential functions of the three DNA-PK components...
  12. ncbi IgH class switch recombination to IgG1 in DNA-PKcs-deficient B cells
    John P Manis
    Howard Hughes Medical Institute and Children s Hospital, Center for Blood Research and Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Immunity 16:607-17. 2002
    ..DP-T/HC/LC mice were severely deficient for all serum IgH isotypes except IgM and, unexpectedly, IgG1...
  13. ncbi Interallelic class switch recombination contributes significantly to class switching in mouse B cells
    Stéphane Reynaud
    Laboratoire d Immunologie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6101, Equipe labellisée, La Ligue, Faculte de Medecine, Limoges, France
    J Immunol 174:6176-83. 2005
    ....
  14. ncbi Autoantigen-B cell antigen receptor interactions that regulate expression of B cell antigen receptor Loci
    Xiaohe Liu
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Immunol 178:5035-47. 2007
    ..We propose that this adaptive process allows this class of autoreactive B cell to avoid conventional tolerance pathways and promotes development to mature phenotype...
  15. pmc DNA-PKcs and Artemis function in the end-joining phase of immunoglobulin heavy chain class switch recombination
    Sonia Franco
    Howard Hughes Medical Institute, The Children s Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 205:557-64. 2008
    ..In the absence of either factor, B cells activated for CSR frequently generate AID-dependent IgH locus chromosomal breaks and translocations...
  16. ncbi Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation
    F Young
    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts 02115
    Genes Dev 8:1043-57. 1994
    ....
  17. ncbi Targeted insertion of a variable region gene into the immunoglobulin heavy chain locus
    S Taki
    Institute for Genetics, University of Cologne, Germany
    Science 262:1268-71. 1993
    ..Immunoglobulin transgenic mice generated in this fashion by gene targeting should prove useful for the exploration of immunoregulatory mechanisms...
  18. ncbi B cell development under the condition of allelic inclusion
    E Sonoda
    Institute for Genetics, University of Koln, Federal Republic of Germany
    Immunity 6:225-33. 1997
    Mice whose IgH alleles are engineered to encode two distinct antibody heavy (H) chains generate a normal-sized B cell compartment in which most cells stably express the two heavy chains...
  19. ncbi In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death
    K P Lam
    Institute for Genetics, University of Cologne, Federal Republic of Germany
    Cell 90:1073-83. 1997
    ..Ablation leads to rapid death of mature B lymphocytes, which is preceded by down-regulation of MHC antigens and up-regulation of CD95 (Fas) and can be delayed by constitutive bcl-2 expression...
  20. ncbi Receptor editing in a transgenic mouse model: site, efficiency, and role in B cell tolerance and antibody diversification
    R Pelanda
    Institute for Genetics, University of Koln, Germany
    Immunity 7:765-75. 1997
    Mice carrying transgenic rearranged V region genes in their IgH and Igkappa loci to encode an autoreactive specificity direct the emerging autoreactive progenitors into a pre-B cell compartment, in which their receptors are edited by ..
  21. pmc Ku80 is required for immunoglobulin isotype switching
    R Casellas
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    EMBO J 17:2404-11. 1998
    ..Thus, Ku80 is essential for switch recombination in vivo, suggesting a significant overlap between the molecular machinery that mediates DNA DSB repair, V(D)J recombination and isotype switching...
  22. ncbi Ig heavy chain class switching in Rag-deficient mice
    R Lansford
    Howard Hughes Medical Institute, The Children s Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Int Immunol 10:325-32. 1998
    ..Together, these findings indicate that neither RAG-1 nor RAG-2 expression is required for efficient class switching to most HC isotypes in B cells...
  23. pmc Ku70 is required for late B cell development and immunoglobulin heavy chain class switching
    J P Manis
    Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    J Exp Med 187:2081-9. 1998
    ..We conclude that Ku70 is required for the generation of B cells that have undergone Ig HC class switching. Potential roles for Ku70 in the CSR process are discussed...
  24. ncbi Somatic hypermutation in the heavy chain locus correlates with transcription
    Y Fukita
    Institute for Genetics, University of Cologne, Germany
    Immunity 9:105-14. 1998
    Three mutant immunoglobulin heavy chain (IgH) insertion mice were generated in which a targeted nonfunctional IgH passenger transgene was either devoid of promoter (pdelta) or was placed under the transcriptional control of either its ..
  25. pmc Different sensitivity to receptor editing of B cells from mice hemizygous or homozygous for targeted Ig transgenes
    U Braun
    Max Planck Institute for Immunobiology, Stübeweg 51 D 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 97:7429-34. 2000
    ....
  26. ncbi Deletion of the DQ52 element within the Ig heavy chain locus leads to a selective reduction in VDJ recombination and altered D gene usage
    L Nitschke
    Institute of Virology and Immunobiology, University of Wurzburg, Wurzburg, Germany
    J Immunol 166:2540-52. 2001
    ..We propose a model in which the DQ52 promoter region enhances the induction of secondary DJ rearrangements...
  27. ncbi Localization of the 3' IgH locus elements that effect long-distance regulation of class switch recombination
    E Pinaud
    Laboratoire d Immunologie, CNRS UMR6101, Faculte de Medecine, Limoges, France
    Immunity 15:187-99. 2001
    ..in the 3' region have shown that both hs3a and hs1,2 are dispensable for normal expression and rearrangement of the IgH locus but that their replacement with a transcribed neo gene severely affects class switch recombination...
  28. ncbi Role of antigen receptor affinity in T cell-independent antibody responses in vivo
    Tien An Yang Shih
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    Nat Immunol 3:399-406. 2002
    ....
  29. ncbi Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement
    I Hsin Su
    Laboratory of Lymphocyte Signaling, The Rockefeller University, New York, NY 10021, USA
    Nat Immunol 4:124-31. 2003
    ..a critical role for Ezh2 in early B cell development and rearrangement of the immunoglobulin heavy chain gene (Igh). We also revealed Ezh2 as a key regulator of histone H3 methylation in early B cell progenitors...
  30. pmc B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells
    Tri Giang Phan
    Centenary Institute of Cancer Medicine and Cell Biology, Locked Bag Number 6, Newtown NSW 2042, Australia
    J Exp Med 197:845-60. 2003
    ..That bacteria-derived LPS could circumvent these controls may explain the well-known association between autoantibody-mediated diseases and episodes of systemic infection...
  31. ncbi The immunoglobulin heavy-chain locus hs3b and hs4 3' enhancers are dispensable for VDJ assembly and somatic hypermutation
    Caroline Le Morvan
    Laboratoire d Immunologie, Unité Mixte de Recherche 6101, Centre Nationale de la Recherche Scientifique, Faculte de Medecine, Limoges, France
    Blood 102:1421-7. 2003
    ....
  32. pmc H2AX is required for recombination between immunoglobulin switch regions but not for intra-switch region recombination or somatic hypermutation
    Bernardo Reina-San-Martin
    Laboratory of Molecular Immunology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA
    J Exp Med 197:1767-78. 2003
    ..Our results suggest a role for H2AX in regulating the higher order chromatin remodeling that facilitates switch region synapsis...
  33. ncbi Germ-line transcription occurs on both the functional and the non-functional alleles of immunoglobulin constant heavy chain genes
    Laurent Delpy
    CNRS UMR 6101, Laboratoire d Immunologie, Faculte de Medecine, Limoges, France
    Eur J Immunol 33:2108-13. 2003
    ..Here, we provide evidence that germ-line transcription occurs on both the functional and the non-functional alleles...
  34. ncbi Progressive surface B cell antigen receptor down-regulation accompanies efficient development of antinuclear antigen B cells to mature, follicular phenotype
    Lynn Heltemes-Harris
    Kimmel Cancer Center and Department of Microbiology and Immunology, Jefferson Medical College, Philadelphia, PA 19107, USA
    J Immunol 172:823-33. 2004
    ....
  35. ncbi B cell receptor signal strength determines B cell fate
    Stefano Casola
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 5:317-27. 2004
    ..Mice carrying a targeted replacement of Igh by LMP2A leading to high or low expression of the LMP2A protein developed B-1 or follicular and marginal zone B ..
  36. ncbi Stochastic pairing of Ig heavy and light chains frequently generates B cell antigen receptors that are subject to editing in vivo
    Tatiana Novobrantseva
    Institute for Genetics, University of Cologne, Weyertal 121, D 50931 Cologne, Germany
    Int Immunol 17:343-50. 2005
    ..These data thus suggest that about half of the emerging antibody repertoire is negatively selected during B lymphopoiesis due to the likely encoding of autoreactive or non-functional BCRs...
  37. ncbi Receptor editing can lead to allelic inclusion and development of B cells that retain antibodies reacting with high avidity autoantigens
    Sucai Liu
    Integrated Department of Immunology, National Jewish Medical and Research Center and University of Colorado Health Sciences Center, Denver, CO 80206, USA
    J Immunol 175:5067-76. 2005
    ..These dual Ab-expressing autoreactive B cells conceal their autoantibodies within the cell manifesting a superficially tolerant phenotype that can be partially overcome to secrete IgM autoantibodies...
  38. ncbi A single DH gene segment creates its own unique CDR-H3 repertoire and is sufficient for B cell development and immune function
    Robert L Schelonka
    Department of Pediatrics, University of Alabama, Birmingham, AL 35294, USA
    J Immunol 175:6624-32. 2005
    ..1 in the DH locus. We term this D-limited IgH allele DeltaD-DFL...
  39. pmc Role of genomic instability and p53 in AID-induced c-myc-Igh translocations
    Almudena R Ramiro
    Laboratory of Molecular Immunology, The Rockefeller University, Universidad Autonoma de Madrid, Madrid 28049, Spain
    Nature 440:105-9. 2006
    ..Here we find that translocations between c-myc and the IgH locus (Igh) are induced in primary B cells within hours of expression of the catalytically active form of ..
  40. ncbi Regulation of IgH gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination
    Roshi Afshar
    Department of Microbiology Immunology, Vanderbilt University Medical School, Nashville, TN 37232, USA
    J Immunol 176:2439-47. 2006
    ..Paradoxically, germline deletions of the IgH enhancer (Emu) only modestly reduce D(H)-->J(H) rearrangements when assessed in peripheral B cells...
  41. ncbi Antibody class switching mediated by yeast endonuclease-generated DNA breaks
    Ali A Zarrin
    Howard Hughes Medical Institute, Children s Hospital, CBR Institute for Biomedical Research, and Department of Genetics, Harvard University Medical School, Boston, MA 02115, USA
    Science 315:377-81. 2007
    ..cytidine deaminase (AID)-dependent double-strand breaks (DSBs) within two large immunoglobulin heavy chain (IgH) locus switch (S) regions that lie up to 200 kilobases apart...
  42. pmc IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig alpha/beta
    Ari Waisman
    Institute for Genetics, University of Cologne, 50674 Cologne, Germany
    J Exp Med 204:747-58. 2007
    ..gamma1-driven B cell development is disfavored in competition with developing B cells expressing a wild-type (WT) IgH locus...
  43. pmc Yin Yang 1 is a critical regulator of B-cell development
    Huifei Liu
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 21:1179-89. 2007
    ..ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a ..
  44. pmc Antisense intergenic transcription precedes Igh D-to-J recombination and is controlled by the intronic enhancer Emu
    Daniel J Bolland
    Laboratory of Chromatin and Gene Expression, Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, United Kingdom
    Mol Cell Biol 27:5523-33. 2007
    ..We previously proposed that antisense intergenic transcription, activated throughout the mouse Igh VH region in pro-B cells, remodels chromatin for VH-to-DJH recombination...
  45. ncbi Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function
    Alexandra Schebesta
    Research Institute of Molecular Pathology, Vienna Biocenter, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Immunity 27:49-63. 2007
    ....
  46. pmc Chromosomal position of a VH gene segment determines its activation and inactivation as a substrate for V(D)J recombination
    Jamie Geier Bates
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Exp Med 204:3247-56. 2007
    ..These data suggest that locus contraction, mimicked by proximal targeting, can override any regulation imposed by DNA sequences immediately surrounding V(H) gene segments...
  47. pmc Association between the Igk and Igh immunoglobulin loci mediated by the 3' Igk enhancer induces 'decontraction' of the Igh locus in pre-B cells
    Susannah L Hewitt
    Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    Nat Immunol 9:396-404. 2008
    Variable-(diversity)-joining (V(D)J) recombination at loci encoding the immunoglobulin heavy chain (Igh) and immunoglobulin light chain (Igk) takes place sequentially during successive stages in B cell development...
  48. pmc The 3D structure of the immunoglobulin heavy-chain locus: implications for long-range genomic interactions
    Suchit Jhunjhunwala
    Division of Biological Sciences, 0377, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 133:265-79. 2008
    The immunoglobulin heavy-chain (Igh) locus is organized into distinct regions that contain multiple variable (V(H)), diversity (D(H)), joining (J(H)) and constant (C(H)) coding elements...
  49. pmc AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations
    Davide F Robbiani
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    Cell 135:1028-38. 2008
    ..of the most well characterized oncogenic translocations juxtaposes c-myc and the immunoglobulin heavy-chain locus (IgH) and is found in Burkitt's lymphomas in humans and plasmacytomas in mice...
  50. pmc Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes
    Elsa Callen
    Experimental Immunology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Mol Cell 34:285-97. 2009
    ..Our experiments reveal a DNA-PKcs-dependent pathway that regulates DNA repair and activation of p53 in the absence of ATM...
  51. pmc Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation
    Tri Giang Phan
    1 Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco UCSF, California, USA 2 Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia
    Nat Immunol 10:786-93. 2009
    ..Thus, we characterize SCS macrophages as specialized antigen-presenting cells functioning at the apex of an antigen transport chain that promotes humoral immunity...
  52. pmc PI3 kinase signals BCR-dependent mature B cell survival
    Lakshmi Srinivasan
    Program of Cellular and Molecular Medicine, Children s Hospital, and Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 139:573-86. 2009
    ....
  53. doi In vivo redundant function of the 3' IgH regulatory element HS3b in the mouse
    Anne Gaelle Bebin
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche, Universite de Limoges, Limoges, France
    J Immunol 184:3710-7. 2010
    In the mouse, the regulatory region located at the 3' end of the IgH locus includes four transcriptional enhancers: HS3a, HS1-2, HS3b, and HS4; the first three lie in a quasi-palindromic structure...
  54. pmc 53BP1 regulates DNA resection and the choice between classical and alternative end joining during class switch recombination
    Anne Bothmer
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 207:855-65. 2010
    ..We explore the role of 53BP1 in intrachromosomal DNA repair using I-SceI to introduce paired DSBs in the IgH locus...
  55. pmc The in vivo pattern of binding of RAG1 and RAG2 to antigen receptor loci
    Yanhong Ji
    Department of Immunobiology, Yale University School of Medicine, 300 Cedar Street, Box 208011, New Haven, CT 06520 8011, USA
    Cell 141:419-31. 2010
    ..a highly focal manner to a small region of active chromatin encompassing Ig kappa and Tcr alpha J gene segments and Igh and Tcr beta J and J-proximal D gene segments...
  56. doi Genomic deletion of the whole IgH 3' regulatory region (hs3a, hs1,2, hs3b, and hs4) dramatically affects class switch recombination and Ig secretion to all isotypes
    Christelle Vincent-Fabert
    Unité Mixte de Recherche Centre National de la Recherche Scientifique, Universite de Limoges, 2 rue du Dr Marcland, Limoges Cedex, France
    Blood 116:1895-8. 2010
    The immunoglobulin heavy chain locus (IgH) undergoes multiple changes along B-cell differentiation. In progenitor B cells, V(D)J assembly allows expression of μ heavy chains...
  57. pmc Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter
    Gabriel D Victora
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    Cell 143:592-605. 2010
    ..Thus, T cell help, and not direct competition for antigen, is the limiting factor in GC selection...
  58. pmc CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells
    Stephanie C Degner
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:9566-71. 2011
    Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all V(H) genes to be brought in proximity with D(H)-J(H) segments to create a diverse antibody repertoire, but the proteins directly ..
  59. doi Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity
    Masahiro Kitano
    Research Unit for Immunodynamics, RIKEN, Research Center for Allergy and Immunology, Yokohama, 230 0045, Japan
    Immunity 34:961-72. 2011
    ..These results clarify the role of Bcl6 in pre-GC B cell dynamics and highlight the modulation of Bcl6 expression in Tfh cells that persist in the late phase of the antibody response...
  60. pmc Pro-B cells sense productive immunoglobulin heavy chain rearrangement irrespective of polypeptide production
    Johannes Lutz
    Division of Molecular Immunology, Department of Internal Medicine III, Nikolaus Fiebiger Center of Molecular Medicine, Friedrich Alexander University Erlangen Nurnberg, D 91054 Erlangen, Germany
    Proc Natl Acad Sci U S A 108:10644-9. 2011
    ..Ig rearrangement begins in pro-B cells at the IgH locus...
  61. pmc Cross talk between immunoglobulin heavy-chain transcription and RNA surveillance during B cell development
    Aurélien Tinguely
    Universite de Limoges, CNRS UMR 6101, Limoges, France
    Mol Cell Biol 32:107-17. 2012
    ..Here, we tracked transcription and posttranscriptional regulation for both Ig heavy-chain (IgH) alleles in mice carrying a nonfunctional knock-in allele...
  62. doi A p53 defect sensitizes various stages of B cell development to lymphomagenesis in mice carrying an IgH 3' regulatory region-driven c-myc transgene
    Remi Fiancette
    Faculte de Medecine, Unité Mixte de Recherche 6101, Centre National de la Recherche Scientifique, 87025 Limoges, France
    J Immunol 187:5772-82. 2011
    ..After translocation into the IgH locus, c-myc is constitutively expressed under the control of active IgH enhancers...
  63. doi AID-driven deletion causes immunoglobulin heavy chain locus suicide recombination in B cells
    Sophie Peron
    Limoges University, CNRS, 2 rue Marcland, 87025 Limoges Cedex, France
    Science 336:931-4. 2012
    ..The frequency of this event is approaching that of class switching and makes it a potential regulator of B cell homeostasis...
  64. pmc The IgH 3' regulatory region controls somatic hypermutation in germinal center B cells
    Pauline Rouaud
    Centre National de la Recherche Scientifique CNRS UMR 7276, Universite de Limoges, 87025 Limoges, France
    J Exp Med 210:1501-7. 2013
    ..The contribution of IgH transcriptional enhancers in SHM is unclear...
  65. ncbi Regulatory elements in the immunoglobulin heavy chain gene 3'-enhancers induce c-myc deregulation and lymphomagenesis in murine B cells
    Jinghong Wang
    Center for Molecular Biology in Medicine, Veterans Affairs Palo Alto Health Care System and the Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 280:12766-73. 2005
    ..translocations that juxtapose the c-myc proto-oncogene with regulatory elements of the immunoglobulin heavy (IgH) or light chain loci resulting in the deregulation of c-myc expression...
  66. pmc Sites that direct nuclear compartmentalization are near the 5' end of the mouse immunoglobulin heavy-chain locus
    Qiaoxin Yang
    Department of Cell Biology CH 416, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA
    Mol Cell Biol 25:6021-30. 2005
    VDJ rearrangement in the mouse immunoglobulin heavy chain (Igh) locus involves a combination of events, including a large change in its nuclear compartmentalization...
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    Anja E Hauser
    Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06511, USA
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    Center for Immunology, Department of Medicine, University of Minnesota Medical School, University of Minnesota, Minneapolis, MN 55455, USA
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    Centre National de la Recherche Scientifique Unité Mixte de Recherche 6101, Universite de Limoges, France
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    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts
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    Institute for Genetics, University of Cologne, Federal Republic of Germany
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    Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
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  76. pmc Two murine natural polyreactive autoantibodies are encoded by nonmutated germ-line genes
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    Department of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada
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    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
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  79. pmc CC chemokine receptor 6 expression by B lymphocytes is essential for the development of isolated lymphoid follicles
    Keely G McDonald
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
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    Petra J ter Brugge
    Departments of Immunology, Erasmus MC Rotterdam, Rotterdam, The Netherlands
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    Dominique Gatto
    Garvan Institute of Medical Research, Darlinghurst NSW 2010, Australia
    Immunity 31:259-69. 2009
    ..EBI2 therefore provides a previously uncharacterized dimension to B cell migration that is crucial for coordinating rapid versus long-term antibody responses...
  82. pmc IL-6 and MYC collaborate in plasma cell tumor formation in mice
    Sebastian Rutsch
    Medizinische Klinik V, University of Heidelberg, and National Center for Tumor Diseases, Heidelberg, Germany
    Blood 115:1746-54. 2010
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    Cristina L Swanson
    Integrated Department of Immunology, University of Colorado, Denver, CO 80206, USA
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  84. pmc Comparison of identical and functional Igh alleles reveals a nonessential role for Eμ in somatic hypermutation and class-switch recombination
    Fubin Li
    Department of Biological Sciences, Hunter College and Graduate Center, City University of New York, New York, NY 10065, USA
    J Immunol 185:6049-57. 2010
    ..The current study circumvented this problem by modifying the murine Igh locus through simultaneous insertion of a fully assembled V(H) gene and deletion of Eμ...
  85. pmc ATM damage response and XLF repair factor are functionally redundant in joining DNA breaks
    Shan Zha
    Howard Hughes Medical Institute, The Children s Hospital, the Immune Disease Institute and the Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 469:250-4. 2011
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    Department of Molecular Biology, University of Innsbruck Medical School, Austria
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    T Shimizu
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    EMBO J 11:4869-75. 1992
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    Division of Biology, California Institute of Technology, Pasadena 91125
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    Nature 283:35-40. 1980
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    Department of Immunology, University of Toronto, Canada
    Mol Cell Biol 15:671-81. 1995
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  93. ncbi The complete murine immunoglobulin class switch region of the alpha heavy chain gene-hierarchic repetitive structure and recombination breakpoints
    H Arakawa
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
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  94. pmc B cell antigen receptor specificity and surface density together determine B-1 versus B-2 cell development
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    Institute of Molecular and Cell Biology, The National University of Singapore, Singapore 117609, Republic of Singapore
    J Exp Med 190:471-7. 1999
    ..the immunoglobulin (Ig) heavy (H) chain variable (V) region from a rearranged V(H)12 gene inserted into the IgH locus generate predominantly B-1 cells, whereas expression of two other V(H) region transgenes (V(H)B1-8 and V(H)..
  95. ncbi Memory B-cell persistence is independent of persisting immunizing antigen
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    Institute for Genetics, University of Cologne, Germany
    Nature 407:636-42. 2000
    ....
  96. ncbi DNA double-strand breaks in immunoglobulin genes undergoing somatic hypermutation
    L Bross
    Basel Institute for Immunology, Grenzacherstrasse 487, CH 4005 Basel, Switzerland
    Immunity 13:589-97. 2000
    ..Using VDJ passenger Ig heavy chain (IgH) knockin mouse strains, we now demonstrate a high frequency of DNA double-strand breaks (DSBs) in the targeted VDJ ..
  97. ncbi Role of CD40 in a T cell-mediated negative regulation of Ig production
    L Majlessi
    Unité d Immunophysiologie Moléculaire, Institut Pasteur, Paris, France
    J Immunol 166:841-7. 2001
    ..T splenocytes from IGH(a/a) mice are able in vivo to totally and chronically inhibit the production of IgG(2a)(b) (IgG2a from the IGH(b) ..
  98. pmc Indirect and direct evidence for DNA double-strand breaks in hypermutating immunoglobulin genes
    H Jacobs
    Basel Institute for Immunology, Switzerland
    Philos Trans R Soc Lond B Biol Sci 356:119-25. 2001
    ..Using mouse strains that carry a non-functional, pre-defined V(H)D(H)J(H) gene segment in their IgH locus we demonstrate DNA double-strand breaks (DSBs) in and around V(H)D(H)J(H) in B cells undergoing somatic ..
  99. pmc Secondary heavy chain rearrangement: a mechanism for generating anti-double-stranded DNA B cells
    Debora R Sekiguchi
    Division of Rheumatology, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Exp Med 197:27-39. 2003
    ..Genetic analysis associated this response with the generation of anti-dsDNA B cells through secondary rearrangements that replaced the site-directed transgene (sd-tg) with endogenous VH genes...
  100. ncbi Combination of 3' and 5' IgH regulatory elements mimics the B-specific endogenous expression pattern of IgH genes from pro-B cells to mature B cells in a transgenic mouse model
    Laurence Guglielmi
    UMR CNRS 6101, Faculte de Medecine, 2 rue Dr Marcland, 87025, Limoges, France
    Biochim Biophys Acta 1642:181-90. 2003
    ..stage specificity of the intronic Emu element and of the locus control region (LCR) that lies downstream of the IgH chain locus, we generated transgenic mice harboring a V(H) promoter-GFP reporter gene linked to the 3'LCR region ..
  101. pmc Developmental separation of V(D)J recombinase expression and initiation of IgH recombination in B lineage progenitors in vivo
    Lisa Borghesi
    University of Massachusetts Medical School, Molecular Genetics and Microbiology, 55 Lake Ave North, Worcester 01655, USA
    J Exp Med 199:483-9. 2004
    ..that is not governed by the same chromatin remodeling constraints as endogenous immunoglobulin heavy chain (IgH) loci and examined the requirements for V(D)J recombination to initiate in early B lineage progenitors...