Mbd2

Summary

Gene Symbol: Mbd2
Description: methyl-CpG binding domain protein 2
Alias: MBD2a, methyl-CpG-binding domain protein 2, methyl-CpG-binding protein MBD2, testis-specific methyl-CpG binding protein 2
Species: mouse

Top Publications

  1. pmc Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation
    Alessandro Brero
    Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    J Cell Biol 169:733-43. 2005
  2. ncbi A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
    J Guy
    Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Edinburgh, UK
    Nat Genet 27:322-6. 2001
  3. pmc Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development
    B Hendrich
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, The King s Buildings, Edinburgh EH9 3JR, Scotland
    Genes Dev 15:710-23. 2001
  4. pmc Mbd2 contributes to DNA methylation-directed repression of the Xist gene
    Helen Barr
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh, United Kingdom
    Mol Cell Biol 27:3750-7. 2007
  5. pmc Identification and characterization of a family of mammalian methyl-CpG binding proteins
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
    Mol Cell Biol 18:6538-47. 1998
  6. ncbi Gene silencing quantitatively controls the function of a developmental trans-activator
    Anne S Hutchins
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Mol Cell 10:81-91. 2002
  7. pmc Regulation of heterochromatin remodelling and myogenin expression during muscle differentiation by FAK interaction with MBD2
    Shi Wen Luo
    Department of Neurology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    EMBO J 28:2568-82. 2009
  8. ncbi Deficiency of Mbd2 suppresses intestinal tumorigenesis
    Owen J Sansom
    Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK
    Nat Genet 34:145-7. 2003
  9. doi Effect of Methyl-CpG binding domain protein 2 (MBD2) on AMD-like lesions in ApoE-deficient mice
    Jun Ru Pan
    Department of Ophthalmology, Huazhong University of Science and Technology, Wuhan, 430030, China
    J Huazhong Univ Sci Technolog Med Sci 34:408-14. 2014
  10. pmc Decreased levels of miR-224 and the passenger strand of miR-221 increase MBD2, suppressing maspin and promoting colorectal tumor growth and metastasis in mice
    Kefei Yuan
    Department of Biochemistry and Molecular Biology, University of North Dakota, Grand Forks, North Dakota The State Key Laboratory for Biotherapy, West China Hospital, Sichuan University, Chengdu, China
    Gastroenterology 145:853-64.e9. 2013

Research Grants

  1. The Role of MicroRNA in Hepatocarcinogenesis
    Kalpana Ghoshal; Fiscal Year: 2007

Scientific Experts

  • Kalpana Ghoshal
  • Guilin Wang
  • Andrea Fuso
  • Brian Hendrich
  • Denner D R
  • Ellen N Kersh
  • Wolfgang Mann
  • Adrian Bird
  • Jixin Zhong
  • Shu Zhang
  • Xiaoquan Rao
  • Jennifer Berger
  • Anne S Hutchins
  • Hai Jun Li
  • Dhaval Varshney
  • Peter C Cook
  • Megha A Desai
  • Yang Cao
  • Jun Ru Pan
  • Julia M Hum
  • Kefei Yuan
  • M Linke
  • Annette Becker
  • Liqing Wang
  • D R Denner
  • Kimberly P Keil
  • Ping Yang
  • Qilin Yu
  • Cong Yi Wang
  • Seung Gi Jin
  • Owen J Sansom
  • Chun Ling Jiang
  • Gordon D Ginder
  • Careen Katryniok
  • Shu Ying Feng
  • Jessica L MacDonald
  • Isabel Martín Caballero
  • Girdhari Lal
  • Mi Sun Kim
  • M Cristina Cardoso
  • Andreas May
  • Shi Wen Luo
  • Toby J Phesse
  • Nancy T Ruddock-D'Cruz
  • Maren Godmann
  • Helen Barr
  • Ying Chun Lu
  • Jeremy W Rupon
  • Alan R Clarke
  • J Berger
  • Patrick Rodriguez
  • Owen James Sansom
  • Min Wang
  • Alessandro Brero
  • Gerd P Pfeifer
  • Linda Jeffery
  • Adrian P Bird
  • Steven L Reiner
  • Francesca Lembo
  • Chih Lin Hsieh
  • A Bird
  • Boris Kantor
  • Jeffrey M Craig
  • Andrew J Oler
  • Xiao Fang Sun
  • Andrew N J McKenzie
  • Wei Ping Liao
  • Susanna C Fagerholm
  • Rachel J Lundie
  • David C Williams
  • Jim Selfridge
  • Yongping Cai
  • J Neel Scarsdale
  • Alasdair C Ivens
  • Thomas Clouaire
  • Heather D Webb
  • Ninad M Walavalkar
  • Shu Jing Liu
  • Xiaoyu Ma
  • Robert J White
  • Gareth Rhys Jones
  • De Huang
  • Graham D Thomas
  • Yue Sheng Long
  • Elisabeth Wachter
  • Victoria H Cowling
  • Alexander T Phythian-Adams
  • Lucy H Jones
  • Vicky L Morrison
  • Leander M Sinanan

Detail Information

Publications56

  1. pmc Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation
    Alessandro Brero
    Department of Biology II, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    J Cell Biol 169:733-43. 2005
    ..Similar to MeCP2, another methyl CpG-binding protein, MBD2, also increased during myogenic differentiation and could induce clustering of pericentric regions, arguing for ..
  2. ncbi A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
    J Guy
    Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Edinburgh, UK
    Nat Genet 27:322-6. 2001
    ....
  3. pmc Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development
    B Hendrich
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Michael Swann Building, The King s Buildings, Edinburgh EH9 3JR, Scotland
    Genes Dev 15:710-23. 2001
    b>MBD2 and MBD3 are closely related proteins with consensus methyl-CpG binding domains. MBD2 is a transcriptional repressor that specifically binds to methylated DNA and is a component of the MeCP1 protein complex...
  4. pmc Mbd2 contributes to DNA methylation-directed repression of the Xist gene
    Helen Barr
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh, United Kingdom
    Mol Cell Biol 27:3750-7. 2007
    ..The absence of Mbd2 caused significant low-level reactivation of Xist, but silencing was restored by exogenous Mbd2...
  5. pmc Identification and characterization of a family of mammalian methyl-CpG binding proteins
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
    Mol Cell Biol 18:6538-47. 1998
    ..We describe here three novel human and mouse proteins (MBD2, MBD3, and MBD4) that contain the methyl-CpG binding domain...
  6. ncbi Gene silencing quantitatively controls the function of a developmental trans-activator
    Anne S Hutchins
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Mol Cell 10:81-91. 2002
    ..We examined cells lacking methyl CpG binding domain protein-2 (MBD2), a molecule that has been proposed to link DNA methylation to silent chromatin...
  7. pmc Regulation of heterochromatin remodelling and myogenin expression during muscle differentiation by FAK interaction with MBD2
    Shi Wen Luo
    Department of Neurology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    EMBO J 28:2568-82. 2009
    ..Here, we report a new signalling of FAK in regulating chromatin remodelling by its interaction with MBD2 (methyl CpG-binding protein 2), underlying FAK regulation of myogenin expression and muscle differentiation...
  8. ncbi Deficiency of Mbd2 suppresses intestinal tumorigenesis
    Owen J Sansom
    Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK
    Nat Genet 34:145-7. 2003
    Gene silencing through de novo methylation of CpG island promoters contributes to cancer. We find that Mbd2, which recruits co-repressor complexes to methylated DNA, is essential for efficient tumorigenesis in the mouse intestine...
  9. doi Effect of Methyl-CpG binding domain protein 2 (MBD2) on AMD-like lesions in ApoE-deficient mice
    Jun Ru Pan
    Department of Ophthalmology, Huazhong University of Science and Technology, Wuhan, 430030, China
    J Huazhong Univ Sci Technolog Med Sci 34:408-14. 2014
    The role of methyl-CpG binding domain protein 2 (MBD2) in an ApoE-deficient mouse model of age-related macular degeneration (AMD) was investigated...
  10. pmc Decreased levels of miR-224 and the passenger strand of miR-221 increase MBD2, suppressing maspin and promoting colorectal tumor growth and metastasis in mice
    Kefei Yuan
    Department of Biochemistry and Molecular Biology, University of North Dakota, Grand Forks, North Dakota The State Key Laboratory for Biotherapy, West China Hospital, Sichuan University, Chengdu, China
    Gastroenterology 145:853-64.e9. 2013
    ..We screened for miRs and miR* with levels that were altered in metastatic colorectal cancer (CRC) cells and human tumor samples and investigated their targets and effects on cell function and tumor progression in mice...
  11. pmc Catalog of mRNA expression patterns for DNA methylating and demethylating genes in developing mouse lower urinary tract
    Kimberly P Keil
    Department of Comparative Biosciences, University of Wisconsin Madison, 1656 Linden Dr, Madison, WI 53706, USA
    Gene Expr Patterns 13:413-24. 2013
    ..Future investigation into how DNA methylation patterns are established, maintained and remodeled during the course of embryonic prostatic bud formation may provide insight into prostate morphogenesis and disease...
  12. pmc Mbd2 promotes foxp3 demethylation and T-regulatory-cell function
    Liqing Wang
    Division of Transplantation Immunology, Department of Pathology and Laboratory Medicine and Biesecker Center for Pediatric Liver Diseases
    Mol Cell Biol 33:4106-15. 2013
    ..recruit histone-modifying and chromatin-remodeling complexes to methylated sites, we tested whether targeting of Mbd2 might promote demethylation of Foxp3 and thereby promote Treg numbers or function...
  13. pmc Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression
    Julia M Hum
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America
    PLoS ONE 9:e97942. 2014
    ..in DNA methylation and the interpretation of DNA methylation patterns -methyl-CpG-binding domain protein 2 (MBD2)...
  14. doi MBD2 regulates TH17 differentiation and experimental autoimmune encephalomyelitis by controlling the homeostasis of T-bet/Hlx axis
    Jixin Zhong
    The Center for Biomedical Research, Tongji Hospital, Huazhong University of Science and Technology, 1095 Jiefang Ave, Wuhan 430030, China
    J Autoimmun 53:95-104. 2014
    ..Here we demonstrated that methyl-CpG-binding domain protein 2 (MBD2), an epigenetic regulator, controls autoimmunity and EAE through T-bet/Hlx...
  15. doi Alteration of Scn3a expression is mediated via CpG methylation and MBD2 in mouse hippocampus during postnatal development and seizure condition
    Hai Jun Li
    Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Collaborative Innovation Center for Neurogenetics and Channelopathies, Institute of Neuroscience, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China Key laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou Medical University, Guangzhou, China
    Biochim Biophys Acta 1849:1-9. 2015
    ..We then demonstrated that the sequence containing -39C was a MBD2-binding motif and the CpG methylation of the promoter region increased the capability of MBD2's binding to the ..
  16. pmc miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency
    Yang Cao
    The CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei, China
    EMBO J 34:609-23. 2015
    ..Mechanistically, we identified Mbd2, a reader for methylated CpGs, as the target of miR-290 cluster that represses glycolysis and reprogramming...
  17. pmc An intrinsically disordered region of methyl-CpG binding domain protein 2 (MBD2) recruits the histone deacetylase core of the NuRD complex
    Megha A Desai
    Department of Human and Molecular Genetics and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
    Nucleic Acids Res 43:3100-13. 2015
    The MBD2-NuRD (Nucleosome Remodeling and Deacetylase) complex is an epigenetic reader of DNA methylation that regulates genes involved in normal development and neoplastic diseases...
  18. pmc SINE transcription by RNA polymerase III is suppressed by histone methylation but not by DNA methylation
    Dhaval Varshney
    1 College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK 2 The MRC Protein Phosphorylation Unit, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nat Commun 6:6569. 2015
    ..The data suggest that methylation of histones rather than DNA plays a dominant role in suppressing SINE transcription. ..
  19. pmc A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells
    Peter C Cook
    Manchester Collaborative Centre for Inflammation Research, University of Manchester, Manchester M3 9NT, UK
    Nat Commun 6:6920. 2015
    ..We demonstrate a key role for the protein methyl-CpG-binding domain-2 (Mbd2), which links DNA methylation to repressive chromatin structure, in regulating expression of a range of genes that ..
  20. pmc Direct homo- and hetero-interactions of MeCP2 and MBD2
    Annette Becker
    Department of Biology, Technische Universitat Darmstadt, Darmstadt, Germany
    PLoS ONE 8:e53730. 2013
    ..We have recently shown that the level of MeCP2 and MBD2, two members of the MBD family, increased during differentiation and their ectopic expression induced ..
  21. pmc A yeast one-hybrid system to screen for methylated DNA-binding proteins
    Shu Ying Feng
    Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japan
    Nucleic Acids Res 38:e189. 2010
    ..We applied the dual-bait system to screen cDNA libraries and demonstrated efficient isolation of clones for methylated DNA-binding proteins. This system would serve as a unique research tool for epigenetics...
  22. doi The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos
    M Linke
    Institute of Human Genetics, Johannes Gutenberg University, Mainz, Germany
    Cytogenet Genome Res 139:71-9. 2013
    ..of ovarian stimulation on the expression of developmentally important reprogramming genes (Apex1, Lig1, Lig3, Mbd2, Mbd3, Mbd4, and Polb) in single early mouse morula embryos (16-cell stage)...
  23. pmc Mi-2/NuRD is required in renal progenitor cells during embryonic kidney development
    D R Denner
    Department of Biochemistry and Molecular Biology, St Louis University, Edward A Doisy Research Center, 1100 S Grand Blvd, St Louis, MO 63104, USA
    Dev Biol 375:105-16. 2013
    ..We also demonstrated that Sall1 and Mi2β exhibited a strong in vivo genetic interaction in the developing kidney. Together these findings indicate that Sall1 and NuRD act cooperatively to maintain CM progenitor cells...
  24. ncbi Dynamic regulation of histone H3 methylation at lysine 4 in mammalian spermatogenesis
    Maren Godmann
    Department of Animal Science, McGill University, Montreal, Quebec, Canada H9X 3V9
    Biol Reprod 77:754-64. 2007
    ..with H3-K4 methylation in the testis, including the association of AOF2 and methyl-CpG-binding domain protein 2 (MBD2a/b) in a complex with histone deacetylase 1 (HDAC1)...
  25. pmc Loss of methyl-CpG-binding domain protein 2 enhances endothelial angiogenesis and protects mice against hind-limb ischemic injury
    Xiaoquan Rao
    Center for Biomedical Research, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave, Wuhan, China
    Circulation 123:2964-74. 2011
    ..We used methyl-CpG-binding domain protein 2 (MBD2), an interpreter for DNA methylome-encoded information, to dissect the impact of DNA methylation on endothelial ..
  26. doi DNA methylase and demethylase activities are modulated by one-carbon metabolism in Alzheimer's disease models
    Andrea Fuso
    Department of Surgery P Valdoni, Sapienza University of Rome, P le Aldo Moro, 5 00185, Rome, Italy
    J Nutr Biochem 22:242-51. 2011
    ..We found that DNA methylases (DNMT1, 3a and 3b) and a putative demethylase (MBD2) were differently modulated, in line with the previously observed changes of PSEN1 methylation pattern in the same ..
  27. doi Single cell RT-PCR on mouse embryos: a general approach for developmental biology
    Wolfgang Mann
    Olympus Life Science Research Europe, Munchen, Germany
    Methods Mol Biol 630:3-12. 2010
    ..It is tempting to speculate that cells expressing the reprogramming machinery have a higher developmental potential...
  28. doi MBD2 and MeCP2 regulate distinct transitions in the stage-specific differentiation of olfactory receptor neurons
    Jessica L MacDonald
    Department of Zoology, Life Sciences Institute and Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    Mol Cell Neurosci 44:55-67. 2010
    ..Here, we show that the MBD proteins MBD2 and MeCP2 regulate distinct transitional stages of olfactory receptor neuron (ORN) differentiation in vivo...
  29. doi DNA demethylation in hormone-induced transcriptional derepression
    Mi Sun Kim
    ERATO, Japan Science and Technology Agency, 4 1 8 Honcho, Kawaguchisi, Saitama 332 0012, Japan
    Nature 461:1007-12. 2009
    ..Thus, the present findings suggest that methylation switching at the DNA level contributes to the hormonal control of transcription...
  30. doi Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity
    Careen Katryniok
    Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt, Germany
    Biochim Biophys Acta 1801:49-57. 2010
    ..Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells...
  31. ncbi Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development
    Nancy T Ruddock-D'Cruz
    Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Mol Reprod Dev 75:48-59. 2008
    ..b>MBD2 was not expressed in murine or bovine oocytes and MeCP2 was not detected in murine blastocysts, subcellular protein ..
  32. pmc The methyl-CpG binding proteins Mecp2, Mbd2 and Kaiso are dispensable for mouse embryogenesis, but play a redundant function in neural differentiation
    Isabel Martín Caballero
    Institute for Stem Cell Research, MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS ONE 4:e4315. 2009
    ..While DNA methylation is known to play important roles in neural specification during embryonic development, no such role has been shown for any of the methyl-CpG binding proteins (Mecps) in mice...
  33. ncbi MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing
    Seung Gi Jin
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 280:12700-9. 2005
    b>MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function...
  34. ncbi Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King s Buildings, Edinburgh EH9 3JR Scotland, UK
    Mamm Genome 10:906-12. 1999
    ..DNA methylation is essential for murine development and is implicated in the control of gene expression. MeCP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of mammalian, nuclear proteins related by the presence in each of an amino acid ..
  35. ncbi Epithelial development and differentiation in the mammary gland is not dependent on alpha 3 or alpha 6 integrin subunits
    T C Klinowska
    School of Biological Sciences, University of Manchester, 3 239 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom
    Dev Biol 233:449-67. 2001
    ..This work demonstrates that mammary tissue genetically lacking major laminin-binding integrin receptors is still able to develop and function...
  36. ncbi Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA
    I Ohki
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, 630 0101, Nara, Japan
    Cell 105:487-97. 2001
    ..The structure indicates how MBD may access nucleosomal DNA without encountering steric interference from core histones, and provides a basis to interpret mutations linked to Rett syndrome in MeCP2...
  37. pmc MBDin, a novel MBD2-interacting protein, relieves MBD2 repression potential and reactivates transcription from methylated promoters
    Francesca Lembo
    Dipartimento di Biologia e Patologia Cellulare e Molecolare L Califano, Istituto di Endocrinologia ed Oncologia Sperimentale del C N R, Universita degli Studi di Napoli Federico II, 80131 Naples, Italy
    Mol Cell Biol 23:1656-65. 2003
    We have identified a human gene encoding a novel MBD2-interacting protein (MBDin) that contains an N-terminal GTP-binding site, a putative nuclear export signal (NES), and a C-terminal acidic region...
  38. ncbi Meddling with methylation
    Chih Lin Hsieh
    Nat Cell Biol 5:502-4. 2003
  39. ncbi Transcriptional regulation of mouse delta-opioid receptor gene by CpG methylation: involvement of Sp3 and a methyl-CpG-binding protein, MBD2, in transcriptional repression of mouse delta-opioid receptor gene in Neuro2A cells
    Guilin Wang
    Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Biol Chem 278:40550-6. 2003
    ..mDOR promoter fragment formed a MeCP1-like protein complex that contained methyl-CpG-binding domain protein 2 (MBD2) and Sp3...
  40. ncbi Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns
    Jeffrey M Craig
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    Hum Mol Genet 12:3109-21. 2003
    ..neocentromere, and the active centromere of a dicentric chromosome, with six of these proteins (Sin3A, PCAF, MYST, MBD2, ORC2, P300/CBP) being demonstrated at mammalian centromeres for the first time...
  41. pmc Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression
    Kalpana Ghoshal
    Department of Molecular and Cellular Biochemistry, College of Medicine, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:6783-93. 2004
    ..Immunofluorescence studies showed that the methyl-CpG-binding proteins, MBD1, MBD2, MBD3, and MeCP2, are localized both in the nuclei and nucleoli of HepG2 cells...
  42. ncbi Expression and localization of components of the histone deacetylases multiprotein repressory complexes in the mouse preimplantation embryo
    Boris Kantor
    Department of Cellular Biochemistry and Human Genetics, The Hebrew University Hadassah Medical School, 91120, Jerusalem, Israel
    Gene Expr Patterns 3:697-702. 2003
    ..MBD3 is a component of the HDAC associated chromatin remodeling complex Mi2/NuRD. The addition of MBD2 to the Mi2/NuRD complex creates MeCP1, a complex that is known to inactivate methylated promoters...
  43. ncbi Components of the DNA methylation system of chromatin control are RNA-binding proteins
    Linda Jeffery
    Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 279:49479-87. 2004
    ..The RNA and methyl-CG binding properties of the MBD proteins are mutually exclusive. We speculate that DNMTs and MBD proteins allow RNA molecules to participate in DNA methylation-mediated chromatin control...
  44. ncbi MBD3L1 is a transcriptional repressor that interacts with methyl-CpG-binding protein 2 (MBD2) and components of the NuRD complex
    Chun Ling Jiang
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 279:52456-64. 2004
    Methyl-CpG-binding domain proteins 2 and 3 (MBD2 and MBD3) are transcriptional repressors that contain methyl-CpG binding domains and are components of a CpG-methylated DNA binding complex named MeCP1...
  45. pmc Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation
    Girdhari Lal
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Immunol 182:259-73. 2009
    ..activated CD4(+) T cells, and peripheral TGFbeta-induced Treg in which it was bound by DNMT1, DNMT3b, MeCP2, and MBD2. Demethylation of this CpG site using the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (Aza) induced ..
  46. ncbi MBD2 deficiency does not accelerate p53 mediated lymphomagenesis
    Owen James Sansom
    Mammalian Genetics, Cardiff School of Biosciences, University of Cardiff, Museum Avenue, PO Box 911, Cardiff CF10 3US, UK
    Oncogene 24:2430-2. 2005
    ..Given our recent finding that loss of methyl-binding domain protein 2 (Mbd2) suppresses intestinal tumorigenesis, we have tested whether loss of Mbd2 increases lymphomagenesis by ..
  47. ncbi Analysis of the Par2 modifier of pulmonary adenoma formation in mice
    Min Wang
    Department of Surgery, Washington University School of Medicine, St Louis, MO, USA
    Exp Lung Res 31:193-204. 2005
    ..The authors recently conducted studies to positionally clone the Par2 gene. This review summarizes the effort and progress towards the identification of Par2 candidates...
  48. pmc GATA-1 forms distinct activating and repressive complexes in erythroid cells
    Patrick Rodriguez
    Department of Cell Biology, Erasmus University Medical Center, Rotterdam, The Netherlands
    EMBO J 24:2354-66. 2005
    ..Based on these findings, we suggest a model for the different roles of GATA-1 in erythroid differentiation...
  49. ncbi Cutting edge: a critical role for gene silencing in preventing excessive type 1 immunity
    Anne S Hutchins
    Abramson Family Cancer Research Institute and Department of Medicine, University of Pennsylvania, Philadelphia, 19104, USA
    J Immunol 175:5606-10. 2005
    ..In the present study, we show that inactivation of the Mbd2 gene, which links DNA methylation and repressed chromatin, results in enhanced resistance to the protozoan parasite ..
  50. ncbi Role of MBD2 in gene regulation and tumorigenesis
    J Berger
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Biochem Soc Trans 33:1537-40. 2005
    ..b>MBD2, a member of the MBD protein family, binds to methylated promoter CpG islands (clusters of high-density CpG ..
  51. pmc Methyl binding domain protein 2 mediates gamma-globin gene silencing in adult human betaYAC transgenic mice
    Jeremy W Rupon
    Massey Cancer Center and Departments of Internal Medicine, Microbiology and Immunology, and Human Genetics, Virginia Commonwealth University, Richmond, VA 23298 0037, USA
    Proc Natl Acad Sci U S A 103:6617-22. 2006
    ..We describe here a role for DNA methylation and MBD2 in the silencing of the human fetal gamma-globin gene...
  52. ncbi Impaired memory CD8 T cell development in the absence of methyl-CpG-binding domain protein 2
    Ellen N Kersh
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Immunol 177:3821-6. 2006
    ..Methyl-CpG-binding domain protein 2 (MBD2) is a transcriptional repressor that binds to methylated DNA and mediates the biological consequences of epigenetic ..
  53. ncbi Cyclophilin a protects Peg3 from hypermethylation and inactive histone modification
    Ying Chun Lu
    Dental Research Institute, UCLA School of Dentistry and Department of Human Genetics, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 281:39081-7. 2006
    ..of acetylation and a gain of lysine 9 trimethylation in histone 3, as well as enhanced DNA methyltransferase 1 and MBD2 binding on the cytosine-guanine dinucleotide (CpG) islands of Peg3...
  54. pmc MBD2 is required for correct spatial gene expression in the gut
    Jennifer Berger
    Wellcome Trust Centre for Cell Biology, Edinburgh University, The King s Buildings, Mayfield Road, Edinburgh, United Kingdom
    Mol Cell Biol 27:4049-57. 2007
    ..Analysis of gene expression in colons from mice lacking the methyl-CpG binding repressor MBD2 revealed frequent activation of genes that are normally only expressed in the exocrine pancreas and duodenum...
  55. pmc Deficiency of Mbd2 attenuates Wnt signaling
    Toby J Phesse
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3US, United Kingdom
    Mol Cell Biol 28:6094-103. 2008
    We have previously shown that deficiency of the methyl binding domain protein Mbd2 dramatically reduces adenoma burden on an Apc(Min/+) background...
  56. doi Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres
    Andreas May
    Institute for Human Genetics, Johannes Gutenberg University Mainz, 55101 Mainz, Germany
    Biol Reprod 80:194-202. 2009
    ..Immunolocalization of DNMT3A, MBD3, APEX1, and LIG3 in most or all nuclei of 40-60-cell embryos is a good indicator of functional activity of genes that are activated by the 16-cell stage...

Research Grants1

  1. The Role of MicroRNA in Hepatocarcinogenesis
    Kalpana Ghoshal; Fiscal Year: 2007
    ..Further, this proposal fits well with the mission/goals set forth by multiple institutes that include NCI, NIDDK and NIAAA n the etiology, prevention and treatment of hepatocellular carcinomas. [unreadable] [unreadable] [unreadable]..