Genomes and Genes
Gene Symbol: Pou5f1
Description: POU domain, class 5, transcription factor 1
Alias: NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, POU domain, class 5, transcription factor 1, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
Publications169 found, 100 shown here
- The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cellsKaoru Mitsui
Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
Cell 113:631-42. 2003..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
- Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissuesKonrad Hochedlinger
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 121:465-77. 2005..These data show that certain adult progenitors remain competent to interpret key embryonic signals and support the notion that progenitor cells are a driving force in tumorigenesis...
- A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryoM H Rosner
Metabolism Branch, National Cancer Institute, Bethesda, Maryland 20892
Nature 345:686-92. 1990..The oct-3 gene is also expressed in primordial germ cells and in the female germ line...
- Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiationHitoshi Niwa
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
Cell 123:917-29. 2005..can be induced to differentiate into the TE lineage by forced repression of the POU-family transcription factor, Oct3/4...
- Conserved POU binding DNA sites in the Sox2 upstream enhancer regulate gene expression in embryonic and neural stem cellsRaffaella Catena
Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, Milano 20126, Italy
J Biol Chem 279:41846-57. 2004..sites are also essential for activity in ES cells, where they bind different members of the POU family, including Oct4, as shown by gel shift assays and chromatin immunoprecipitation with anti-Oct4 antibodies...
- Dissecting self-renewal in stem cells with RNA interferenceNatalia Ivanova
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nature 442:533-8. 2006..Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells...
- Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouseHehai Wang
Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, 1125 MRB IV, 2215 B Garland Avenue, Nashville, TN 37232 0656, USA
Dev Biol 318:112-25. 2008..These results provide the first evidence that ZO-1 is involved in blastocyst formation from the morula by regulating accumulation of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells...
- Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cellsGeppino Falco
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
Dev Biol 307:539-50. 2007..Zscan4 thus seems to be essential for preimplantation development...
- Developmentally regulated expression of mil-1 and mil-2, mouse interferon-induced transmembrane protein like genes, during formation and differentiation of primordial germ cellsSatomi S Tanaka
Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840, Murodo cho, Izumi, Osaka 594 1101, Japan
Mech Dev 119:S261-7. 2002..5-7.5 days post coitum. In migrating PGCs, mil-1 expression was continuously observed and mil-2 expression was first detected during germ cell differentiation...
- Loss of mitogen-activated protein kinase kinase kinase 4 (MAP3K4) reveals a requirement for MAPK signalling in mouse sex determinationDebora Bogani
Mammalian Genetics Unit, Medical Research Council MRC Harwell, Oxfordshire, United Kingdom
PLoS Biol 7:e1000196. 2009....
- The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathwayHui Jye Chen
Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
Genes Dev 20:1933-45. 2006..These results suggest a new role of MACF1 in the Wnt signaling pathway...
- Oct4 expression is not required for mouse somatic stem cell self-renewalChristopher J Lengner
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
Cell Stem Cell 1:403-15. 2007The Pou domain containing transcription factor Oct4 is a well-established regulator of pluripotency in the inner cell mass of the mammalian blastocyst as well as in embryonic stem cells...
- Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancersAttila Remenyi
Gene Expression Program, EMBL, 69117 Heidelberg, Germany
Genes Dev 17:2048-59. 2003..In this study, we focus on two transcription factors, Oct4 and Sox2, as their combination on DNA is considered to direct the establishment of the first three lineages in the ..
- T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the transcriptional control of multiple lineage pathwaysWai Leong Tam
Stem Cell and Developmental Biology, Genome Institute of Singapore, 02 01, Genome, Singapore
Stem Cells 26:2019-31. 2008..Notably, Tcf3 binds to and represses the Oct4 promoter, and this repressive effect requires both the Groucho and CtBP interacting domains of Tcf3...
- A male-specific role for p38 mitogen-activated protein kinase in germ cell sex differentiation in miceKatherine Ewen
Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia
Biol Reprod 83:1005-14. 2010..Inhibition of p38 MAPK signaling in XY germ cells ex vivo reduced expression of the pluripotency marker POU5F1 and increased the expression of Stra8 and SYCP3, premeiosis and meiosis markers, respectively, to levels ..
- Dax1 up-regulates Oct4 expression in mouse embryonic stem cells via LRH-1 and SRAVictoria R Kelly
Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
Mol Endocrinol 24:2281-91. 2010..Therefore, we examined the effect of Dax1 on the LRH-1-mediated activation of the critical ES cell factor Oct4 (Pou5f1)...
- Mesd encodes an LRP5/6 chaperone essential for specification of mouse embryonic polarityJen Chih Hsieh
Department of Biochemistry and Cell Biology, Center for Developmental Genetics, State University of New York, Stony Brook, Stony Brook, NY 11794, USA
Cell 112:355-67. 2003....
- Smad5 determines murine amnion fate through the control of bone morphogenetic protein expression and signalling levelsErika A Bosman
Department of Developmental Biology VIB7 for Biotechnology VIB and Laboratory of Molecular Biology Celgen, University of Leuven, B 3000 Leuven, Belgium
Development 133:3399-409. 2006..5 in Smad5 mutant mice. However, ectopic Oct4-positive (Oct4(+)) and alkaline phosphatase-positive (AP(+)) cells appear suddenly in thickened amnion at E8...
- Dppa2 and Dppa4 are closely linked SAP motif genes restricted to pluripotent cells and the germ lineJoanna Maldonado-Saldivia
Gurdon Institute, Wellcome Trust Cancer Research United Kingdom, Cambridge, United Kingdom
Stem Cells 25:19-28. 2007..The study of expression and function of pluripotent markers such as Dppa2 and Dppa4 is likely to unveil new aspects of the regulation of pluripotency and germ line development in mammals...
- Oct4 dependence of chromatin structure within the extended Nanog locus in ES cellsDana N Levasseur
Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
Genes Dev 22:575-80. 2008..The homeodomain proteins Nanog and Oct4 are essential for mouse ES cell self-renewal...
- Deficiency of splicing factor 1 suppresses the occurrence of testicular germ cell tumorsRui Zhu
Department of Genetics, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
Cancer Res 70:7264-72. 2010..Our data indicate that Sf1 levels in mouse strains correlate with their incidences of TGCTs and implicate the importance of splicing mechanisms in germ cell tumorigenesis...
- Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cellsTobias Brambrink
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell Stem Cell 2:151-9. 2008Pluripotency can be induced in differentiated murine and human cells by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc...
- Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocystsKai Wang
Developmental Epigenetics Laboratory, Department of Animal Science, Michigan State University, East Lansing, Michigan, United States of America
PLoS ONE 5:e10622. 2010..cell mass (ICM) and trophectoderm is governed by the mutually antagonistic effects of the transcription factors Oct4 and Cdx2. Evidence indicates that suppression of Oct4 expression in the trophectoderm is mediated by Cdx2...
- G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesisNirit Feldman
Department of Experimental Medicine, Hebrew University Medical School, Ein Kerem, Jerusalem 91120, Israel
Nat Cell Biol 8:188-94. 2006..Genetic studies show that these epigenetic changes actually have an important role in the inhibition of Oct-3/4 re-expression, thereby preventing reprogramming...
- GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expressionPratik Home
Institute of Maternal Fetal Biology, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
J Biol Chem 284:28729-37. 2009..Our results indicate a novel function of GATA3, in which it is selectively expressed in TE, regulates expression of key genes in TE lineage, and is involved in morula to blastocyst transformation...
- Sexually dimorphic expression of Gata-2 during mouse gonad developmentPam Siggers
MRC Mammalian Genetics Unit, Harwell, OX11 0RD, Didcot, UK
Mech Dev 111:159-62. 2002..Comparative in situ hybridisation using the germ cell marker Oct4 (EMBO J., 8, 2543) indicates that Gata-2 transcripts are restricted to the germ cell lineage at 13.5 dpc.
- SOX8 is expressed during testis differentiation in mice and synergizes with SF1 to activate the Amh promoter in vitroGoslik Schepers
Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072, Australia
J Biol Chem 278:28101-8. 2003....
- Transcriptional regulation of nanog by OCT4 and SOX2David J Rodda
Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
J Biol Chem 280:24731-7. 2005Nanog, Sox2, and Oct4 are transcription factors all essential to maintaining the pluripotent embryonic stem cell phenotype...
- Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic backgroundMatthew S Cook
Department of Cell Biology, Duke University, Durham, NC 27710, USA
Development 138:23-32. 2011..germ cells fail to enter mitotic arrest in G0 and do not downregulate the pluripotency markers NANOG, SOX2 and OCT4. We show that DND1 directly binds a group of transcripts that encode negative regulators of the cell cycle, ..
- Initiation of gastrulation in the mouse embryo is preceded by an apparent shift in the orientation of the anterior-posterior axisAitana Perea-Gomez
Laboratoire de Développement des Vertébrés, Institut Jacques Monod, UMR 7592 CNRS, Universite Paris 6 et 7, 2 place Jussieu, 75251 Paris, France
Curr Biol 14:197-207. 2004..How the establishment of anterior-posterior (AP) polarity relates to the morphology of the postimplantation embryo is, however, unclear...
- PU.1 (Sfpi1), a pleiotropic regulator expressed from the first embryonic stages with a crucial function in germinal progenitorsVirginie Olive
Inserm U636, F 06108, Nice, France
Development 134:3815-25. 2007..5. Transcripts of several germinal markers, including vasa (Mvh, Ddx4), Oct4 (Pou5f1), Dazl and Taf4b, were detected, whereas stella (PGC7, Dppa3) was not. Germ cells of PU...
- New type of POU domain in germ line-specific protein Oct-4H R Scholer
Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Gottingen, FRG
Nature 344:435-9. 1990..Sequence comparison with known POU domains reveals that Oct-4 is a novel member of the POU-family...
- Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3H Yuan
Department of Microbiology, New York University School of Medicine, New York 10016, USA
Genes Dev 9:2635-45. 1995....
- The molecular biology of Oct-4 in the early mouse embryoC E Ovitt
Gene Expression Program, European Molecular Biology Laboratory, Heidelberg, Germany
Mol Hum Reprod 4:1021-31. 1998..Its function as a transcriptional regulator is supported by the identification of an increasing number of potential target genes, including some known to be essential for early embryonic development...
- Activating transcription factor 1 and CREB are important for cell survival during early mouse developmentSusanne C Bleckmann
Department of Molecular Biology of the Cell I, German Cancer Research Centre, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Mol Cell Biol 22:1919-25. 2002..5 due to massive apoptosis. These results indicate that CREB and ATF1 act in concert to mediate signals essential for maintaining cell viability during early embryonic development...
- Oct4 is required for primordial germ cell survivalJames Kehler
Germline Development, Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania, 382 W Street Road, Kennett Square, Pennsylvania 19348, USA
EMBO Rep 5:1078-83. 2004Previous studies have shown that Oct4 has an essential role in maintaining pluripotency of cells of the inner cell mass (ICM) and embryonic stem cells...
- Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cellsKeisuke Kaji
Institute for Stem Cell Research, Centre Development in Stem Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JQ, UK
Development 134:1123-32. 2007..Unlike Mbd3-null ES cells, Mbd3-deficient ICMs grown ex vivo fail to expand their Oct4-positive, pluripotent cell population despite producing robust endoderm outgrowths...
- Novel variants of Oct-3/4 gene expressed in mouse somatic cellsNobuhiko Mizuno
Research Unit for Cell Plasticity, RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
J Biol Chem 283:30997-1004. 2008..Furthermore, we determined novel promoter regions that are sufficient for the expression of Oct-3/4 transcript variants in somatic cells. These findings provide new insights into the postnatal role of Oct-3/4 in somatic tissues...
- Requirement for Wnt3 in vertebrate axis formationP Liu
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Nat Genet 22:361-5. 1999..These studies provide genetic proof for the requirement of Wnt3 in primary axis formation in the mouse...
- Oct-4 knockdown induces similar patterns of endoderm and trophoblast differentiation markers in human and mouse embryonic stem cellsDavid C Hay
Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian, Scotland, United Kingdom
Stem Cells 22:225-35. 2004..Moreover, the upregulation of endoderm-associated markers in both mouse and human ES cells points to overlap between development of trophoblast and endoderm differentiation...
- Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablationGuangjin Pan
Institute of Pharmacology and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
J Biol Chem 279:37013-20. 2004b>OCT4 plays a critical role in maintaining stem cell pluripotency in a dose-dependent manner by activating and repressing multiple downstream genes...
- Oct-4: a germline-specific transcription factor mapping to the mouse t-complexH R Scholer
Max Planck Institute for Biophysical Chemistry, Department of Molecular Cell Biology, Gottingen, FRG
EMBO J 9:2185-95. 1990..Several mouse mutants in the distal region of the mouse t-complex affecting blastocyst and embryonic ectoderm formation also map to this region...
- Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cellsAlexander Meissner
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA
Nat Biotechnol 25:1177-81. 2007..stringent selection for activation of a neomycin-resistance gene inserted into the endogenous Oct4 (also known as Pou5f1) or Nanog loci...
- Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissuesJ Takeda
Howard Hughes Medical Institute, Chicago, IL
Nucleic Acids Res 20:4613-20. 1992..This approach resulted in the amplification of sequences encoding the octamer binding proteins Oct1 and Oct3 (also called Oct4)...
- Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1F M J Lamoury
Department of Neurology, Centre for Immunology, St Vincent s Hospital, Corner West and Boundary Streets, Sydney, NSW 2010, Australia
Cytotherapy 8:228-42. 2006..Indeed, issues such as the identification of a subset of stem cells that is neurally fated, methods of expansion and optimal stage of differentiation for transplantation remain poorly understood...
- A novel octamer binding transcription factor is differentially expressed in mouse embryonic cellsK Okamoto
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan
Cell 60:461-72. 1990..We suggest that Oct-3 is a novel octamer binding transcription factor that is developmentally regulated during mouse embryogenesis...
- Regulation of the Oct-4 gene by nuclear receptorsI Sylvester
EMBL, Gene Expression Programme, Heidelberg, Germany
Nucleic Acids Res 22:901-11. 1994..Specifically, important roles for ARP-1 and RAR in Oct-4 expression are indicated...
- Characterization of a negative retinoic acid response element in the murine Oct4 promoterJ Schoorlemmer
Hubrecht Laboratorium, Netherlands Institute for Developmental Biology, Utrecht
Mol Cell Biol 14:1122-36. 1994Expression of Oct4 in embryonic stem cells is controlled by a distal upstream stem cell-specific enhancer that is deactivated during retinoic acid (RA)-induced differentiation by an indirect mechanism not involving binding of RA receptors ..
- Identification of putative downstream genes of Oct-3, a pluripotent cell-specific transcription factorY Saijoh
Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Japan
Genes Cells 1:239-52. 1996..In order to study how pluripotency is maintained, it is essential to know what genes are regulated by Oct-3...
- Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursorsP Q Thomas
MRC National Institute for Medical Research, London, UK
Development 125:85-94. 1998....
- New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2V Botquin
Gene Expression Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
Genes Dev 12:2073-90. 1998..Expression, DNA binding, and transactivation data are consistent with the hypothesis that OPN expression is regulated by Oct-4 and Sox-2 in preimplantation development...
- Comparative analysis of human, bovine, and murine Oct-4 upstream promoter sequencesV Nordhoff
The Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania 19348, USA
Mamm Genome 12:309-17. 2001..A high number of CCC(A/T)CCC motifs exhibit various levels of homology in these upstream regions. We discuss the importance of these and other sequences and present candidate factors that may bind and regulate Oct-4 gene expression...
- Oct4 distribution and level in mouse clones: consequences for pluripotencyMichele Boiani
Germline Development Group, Center for Animal Transgenesis and Germ Cell Research, The School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, Pennsylvania 19348, USA
Genes Dev 16:1209-19. 2002..The transcription factor Oct4 is expressed during cleavage stages and is essential for the differentiation of the blastocyst...
- The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factorsMasazumi Nishimoto
Division of Developmental Biology, Saitama Medical School Research Center for Genomic Medicine, 1397 1 Yamane Hidaka, Saitama 350 1241, Japan
Biochem Biophys Res Commun 302:581-6. 2003....
- Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse developmentYoshimi Tokuzawa
Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Nara 630 0192, Japan
Mol Cell Biol 23:2699-708. 2003..Transcription factor Oct3/4 is essential for self-renewal of ES cells and early mouse development...
- Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat proteinLee Smith
MRC Mammalian Genetics Unit, Harwell, Didcot, United Kingdom
Dev Dyn 227:600-7. 2003..Maestro maps to a region of mouse chromosome 18 containing a genetic modifier of XX sex reversal. We discuss the possible function of Maestro in light of these data...
- A novel isoform of Vinexin, Vinexin gamma, regulates Sox9 gene expression through activation of MAPK cascade in mouse fetal gonadMakoto Matsuyama
Division of Sex Differentiation, National Institute for Basic Biology, Okazaki 444 8787, Japan
Genes Cells 10:421-34. 2005..Thus, Vinexin gamma seems to be implicated in regulation of Sox9 gene expression by modulating MAPK cascade in mouse fetal gonads...
- Stimulation of Oct-4 activity by Ewing's sarcoma proteinJungwoon Lee
Laboratory of Molecular and Cellular Biology, Department of Life Science, Sogang University, Seoul 121 742, Korea
Stem Cells 23:738-51. 2005..Taken together, our results suggest that Oct-4-mediated transactivation is stimulated by EWS...
- Gonadotropins affect Oct-4 gene expression during mouse oocyte growthManuela Monti
Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita degli Studi di Pavia, Pavia, Italy
Mol Reprod Dev 73:685-91. 2006..Also, they may contribute to our knowledge of the molecular bases of oocyte growth, meiosis resumption, and acquisition of a developmental competence...
- Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cellsYuhki Nakatake
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Minatojima minamimachi 2 2 3, Chu o ku, Kobe 650 0047, Japan
Mol Cell Biol 26:7772-82. 2006Although the POU transcription factor Oct3/4 is pivotal in maintaining self renewal of embryonic stem (ES) cells, little is known of its molecular mechanisms...
- ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250aXiaolin Gao
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Richard Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 105:6656-61. 2008..contributes to the proper expression of numerous genes involved in ES cell self-renewal, including Sox2, Utf1, and Oct4. Furthermore, the pluripotency defects in BAF250a mutant ES cells appear to be cell lineage-specific...
- RBM19 is essential for preimplantation development in the mouseJian Zhang
Division of Gastroenterology, Department of Pediatrics, Medical College of Wisconsin and Children s Research Institute, Milwaukee, WI 53226, USA
BMC Dev Biol 8:115. 2008..Here we analyzed the role of RBM19 during mouse embryonic development by generating mice containing a mutation in the Rbm19 locus via gene-trap insertion...
- The PGD2 pathway, independently of FGF9, amplifies SOX9 activity in Sertoli cells during male sexual differentiationBrigitte Moniot
Department of Genetics and Development, Institut de Génétique Humaine CNRS UPR1142, Montpellier, France
Development 136:1813-21. 2009..This mechanism participates together with FGF9 as an amplification system of Sox9 gene expression and activity during mammalian testicular organogenesis...
- Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2Amy Ralston
Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, Toronto, ON M5G 1L7, Canada
Development 137:395-403. 2010..The transcription factor Cdx2 plays a central role by repressing pluripotency genes, such as Oct4, and promoting extraembryonic trophoblast fate at the blastocyst stage...
- Structure, expression and chromosomal location of the Oct-4 geneY I Yeom
Department of Zoology, University of Texas, Austin
Mech Dev 35:171-9. 1991..By in situ hybridization, detectable onset of zygotic Oct-4 expression does not occur until compaction begins at 8-cells, suggesting that there might be other regulatory factors responsible for initiating Oct-4 expression...
- A dynamic balance between ARP-1/COUP-TFII, EAR-3/COUP-TFI, and retinoic acid receptor:retinoid X receptor heterodimers regulates Oct-3/4 expression in embryonal carcinoma cellsE Ben-Shushan
Hubert H Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University Hadassah Medical School, Jerusalem, Israel
Mol Cell Biol 15:1034-48. 1995....
- Differential expression of the Oct-4 transcription factor during mouse germ cell differentiationM Pesce
EMBL, Gene Expression Programme, Meyerhofstrasse 1, D69012, Heidelberg, Germany
Mech Dev 71:89-98. 1998..The upregulation of Oct-4 in oocytes at the completion of the prophase I of meiotic division further suggests a specific involvement of this transcription factor in oocyte growth or the acquisition of meiotic competence...
- Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryoW R Waldrip
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Cell 92:797-808. 1998..Chimera experiments demonstrate these essential activities are contributed by the extraembryonic tissues. Thus, the extraembryonic tissues play critical roles in establishing the body plan during early mouse development...
- Cripto is required for correct orientation of the anterior-posterior axis in the mouse embryoJ Ding
Center for Advanced Biotechnology and Medicine and Dept of Pediatrics, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
Nature 395:702-7. 1998..Our results indicate that Cripto signalling is essential for the conversion of a proximal-distal asymmetry into an orthogonal anterior-posterior axis...
- Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4J Nichols
Centre for Genome Research, University of Edinburgh, United Kingdom
Cell 95:379-91. 1998b>Oct4 is a mammalian POU transcription factor expressed by early embryo cells and germ cells. We report that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo...
- Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cellsH Niwa
Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
Nat Genet 24:372-6. 2000..The POU transcription factor Oct-3/4 (encoded by Pou5f1) is a candidate regulator in pluripotent and germline cells and is essential for the initial formation of a ..
- A subtractive gene expression screen suggests a role for vanin-1 in testis development in miceJ Bowles
Centre for Molecular and Cellular Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia
Genesis 27:124-35. 2000..This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems...
- Phage display screening reveals an association between germline-specific transcription factor Oct-4 and multiple cellular proteinsC Butteroni
Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, D 69126, Germany
J Mol Biol 304:529-40. 2000..In addition, HMG-1 was found to cooperate with Oct-4 in P19 EC cells. These results provide the first evidence of a non-viral factor that enhances Oct-4 distance-dependent transactivation in stem cells...
- Smad5 is required for mouse primordial germ cell developmentH Chang
Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA
Mech Dev 104:61-7. 2001..To define the role of Smad5 in PGC development, we examined PGC number in Smad5 mutant mice by Oct4 whole-mount in situ hybridization and alkaline phosphatase staining...
- Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cellsHitoshi Niwa
Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita C, Osaka 565 0871, Japan
Mol Cell Biol 22:1526-36. 2002..Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation...
- A unique developmental pattern of Oct-3/4 DNA methylation is controlled by a cis-demodification elementSharon Gidekel
Department of Experimental Medicine and Cancer Research, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
J Biol Chem 277:34521-30. 2002..Methylation of Oct-3/4 may be analogous to methylation of CpG islands on the inactive X chromosome that also occurs at later stages of development...
- The embryonic function of germ cell nuclear factor is dependent on the DNA binding domainZi Jian Lan
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 277:50660-7. 2002..Our results also suggest that GCNF is indeed a transcriptional factor that represses gene transcription mediated via its DBD...
- Multipotent cell lineages in early mouse development depend on SOX2 functionAriel A Avilion
Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
Genes Dev 17:126-40. 2003..b>OCT4 is required for the ICM/epiblast lineage, and at transient high levels for extraembryonic endoderm, but also ..
- Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nucleiAlex Bortvin
Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
Development 130:1673-80. 2003..Mouse embryos that lack an Oct4 gene, which plays an essential role in control of developmental pluripotency, develop to the blastocyst stage and ..
- Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing culturesKoji Shimozaki
Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, 860 0811, Japan
Development 130:2505-12. 2003b>Oct3/4 plays a critical role in maintaining embryonic stem cell pluripotency...
- Nuclear distribution of Oct-4 transcription factor in transcriptionally active and inactive mouse oocytes and its relation to RNA polymerase II and splicing factorsVladimir N Parfenov
Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia 194064
J Cell Biochem 89:720-32. 2003....
- Sexual differentiation of germ cells in XX mouse gonads occurs in an anterior-to-posterior waveDouglas B Menke
Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Dev Biol 262:303-12. 2003..with these results, we find that embryonic ovarian germ cells upregulate the meiotic gene Dmc1 and downregulate the Oct4 transcription factor in an anterior-to-posterior wave...
- Epigenetic control of mouse Oct-4 gene expression in embryonic stem cells and trophoblast stem cellsNaoko Hattori
Laboratory of Cellular Biochemistry, Department of Animal Resource Sciences Veterinary Medical Sciences, The University of Tokyo, Tokyo 113 8657, Japan
J Biol Chem 279:17063-9. 2004..Here we propose that the epigenetic mechanism, consisting of DNA methylation and chromatin remodeling, underlies the developmental stage- and cell type-specific mechanism of Oct-4 gene expression...
- Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitinationHui Ming Xu
Developmental Biology Department, Health Science Center, Shanghai Second Medical University and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 411 He Fei Road, Shanghai 200025, China
J Biol Chem 279:23495-503. 2004..These results reveal that the functional status of Oct-4, in addition to its expression level, dictates its transcriptional activity, and the results open up a new avenue to understand how Oct-4 defines the fate of embryonic stem cells...
- The Wnt co-receptors Lrp5 and Lrp6 are essential for gastrulation in miceOlivia G Kelly
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Development 131:2803-15. 2004..The effect of reducing, but not eliminating, Wnt signaling in Lrp5(+/-);Lrp6(-/-) mutant embryos provides important insight into the interplay between Wnt, Fgf and Nodal signals in patterning the early mouse embryo...
- Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cellsSayaka Okumura-Nakanishi
Laboratory of Molecular and Cellular Assembly, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatuda, Midori ku, Yokohama 226 8501, Japan
J Biol Chem 280:5307-17. 2005..This autoregulatory circuit of the Sox2.Oct-3/4 complex may contribute to maintaining robustly the precise expression level of Oct-3/4 in primitive cells...
- Phenotypic analyses of mouse embryos with ubiquitous expression of Oct4: effects on mid-hindbrain patterning and gene expressionVeronica Ramos-Mejia
Departamento de Fisiologia Molecular y Genetica del Desarrollo, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
Dev Dyn 232:180-90. 2005b>Oct4 is a transcription factor that has been associated with pluripotency and fate determination in the initial cell lineages of mammals...
- Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic developmentPeili Gu
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Mol Cell Biol 25:3492-505. 2005b>Oct4 plays an essential role in maintaining the inner cell mass and pluripotence of embryonic stem (ES) cells...
- Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cellsJoon Lin Chew
Department of Biological Sciences, National University of Singapore
Mol Cell Biol 25:6031-46. 2005..It has previously been shown that Pou5f1, the Oct4 gene, contains a distal enhancer imparting specific expression in both ESCs and preimplantation embryos...
- A novel subpopulation lacking Oct4 expression in the testicular side populationYuko Shimizu
Laboratory Animal Resource Center, University of Tsukuba, Ibaraki 305 8575, Japan
Int J Mol Med 17:21-8. 2006We characterized murine spermatogonial stem cells (SSCs) using a multi-parameter selection strategy, combining Oct4 expression determined by monitoring green fluorescent protein (GFP) expression, and the testicular side population (SP) ..
- The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cellsYuin Han Loh
Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
Nat Genet 38:431-40. 2006b>Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-renewal of embryonic stem (ES) cells...
- Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathwayClaire Chazaud
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
Dev Cell 10:615-24. 2006..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers...
- Use of Chuk as an internal standard suitable for quantitative RT-PCR in mouse preimplantation embryosGeppino Falco
Developmental Genomics and Ageing Section, Laboratory of Genetics, National Institute on Ageing, National Institutes of Health, Baltimore, MD 21224, USA
Reprod Biomed Online 13:394-403. 2006..the utility of this gene as an internal standard for Q-PCR, the expression levels of two known genes (Nalp5/Mater, Pou5f1/Oct3/Oct4) were normalized by Chuk and other housekeeping genes (Actb, Gapdh, Eef1a1, and H2afz) and demonstrated ..
- Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profilingRyo Matoba
Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, United States of America
PLoS ONE 1:e26. 2006POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4...
- Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesisKenjiro Adachi
Institute of Medical Science, University of Tokyo, Minato ku, Toyko 108 8639, Japan
Mol Cell Biol 27:2202-14. 2007..These findings provide evidence for a crucial role of Bysl as an integral factor for ribosome biogenesis and suggest a critical dependence of blastocyst formation on active translation machinery...
- Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1Zhihong Zhang
Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine, Chinese Academy of Sciences, Shanghai, China 200025
FASEB J 21:3042-51. 2007..Our findings provide new evidence for the important role of post-translational modification in controlling Oct-4 function in ES cells...
- Sumoylation of Oct4 enhances its stability, DNA binding, and transactivationFang Wei
Department of Animal Biology, Mari Lowe Center for Comparative Oncology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 282:21551-60. 2007Transcription factor Oct4 is a master regulator affecting the fate of pluripotent stem cells and germ cell precursors...
- Successful co-immunoprecipitation of Oct4 and Nanog using cross-linkingLiying Zhang
Stem Cell Institute, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, Room 2 216, MTRF, 2001 6th Street SE, Minneapolis, MN 55455, USA
Biochem Biophys Res Commun 361:611-4. 2007The transcription factors Oct4 and Nanog are essential for the maintenance of an undifferentiated and pluripotent state in early embryonic cells, embryonic stem cells and embryonal carcinoma cells in humans and mice...
- Retinoic acid, meiosis and germ cell fate in mammalsJosephine Bowles
Division of Molecular Genetics and Development, and ARC Centre of Excellence in Biotechnology and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
Development 134:3401-11. 2007..Although this knowledge clarifies a long-standing mystery in reproductive biology, it also poses many new questions, which we discuss in this review...
- Synergistic function of DNA methyltransferases Dnmt3a and Dnmt3b in the methylation of Oct4 and NanogJing Yu Li
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, People s Republic of China
Mol Cell Biol 27:8748-59. 2007..stem cells and mouse postimplantation embryos, they function synergistically to methylate the promoters of the Oct4 and Nanog genes...
- Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcriptionJungwoon Lee
Department of Life Science, Sogang University, Seoul 121 742, Republic of Korea
Int J Biochem Cell Biol 40:1043-54. 2008..Moreover, ectopic expression of the PKM2 enhanced Oct-4-mediated transcription. These observations indicate that the transactivation potential of the Oct-4 transcription factor is positively modulated by PKM2...
- Body Plan Formation in Early Mouse EmbryoYusuke Marikawa; Fiscal Year: 2004....
- DAB2 AND RAS REGULATIONXiangxi Xu; Fiscal Year: 2001..These mechanistic studies of Dab2 will enhance our understanding of its role in cell growth regulation and the possible involvement in breast and ovary tumorigenicity. ..
- Epithelial Positioning Organization and Ovarian CancerXIANGXI MIKE XU; Fiscal Year: 2010..Such studies will lead to a better understanding of cancer etiology and will contribute to public health by providing cancer preventive stratagy and treatment therapy. ..
- VASCULOGENESIS IN THE MURINE ALLANTOISKAREN DOWNS; Fiscal Year: 2002..Further, results of our experiments will shed light on the mechanism of formation of the endothelial cell lineage. ..
- SHIP PROTEINS AND SIGNAL TRANSDUCTIONLARRY ROHRSCHNEIDER; Fiscal Year: 2003..abstract_text> ..
- Towards renal regenerationMelissa Little; Fiscal Year: 2004..Human ES cell work will be performed using ES01, 02, 03, 04, 05 & 06 listed on the NIH ES cell line registry. ..
- TUMOR CELL BIOLOGY OF THE FMS ONCOGENE PROTEINSLARRY ROHRSCHNEIDER; Fiscal Year: 2005..Overall, the results of these experiments will describe new signaling mechanisms that may be useful for understanding the normal and abnormal development of blood cells and their diseases. ..
- Zic3 and the Control of Body Pattern FormationSTEPHANIE WARE; Fiscal Year: 2005..Through a combination of supervised research, scientific interchange, and selected coursework within this environment, the candidate will obtain the training necessary to transition to an independent investigator. ..
- Role of Par Genes in the Early Mouse EmbryoVERNADETH ALARCON; Fiscal Year: 2006..An understanding of cell polarization in the mouse embryo should have significant benefit for human health research related to reproductive problems. ..
- The role of cell polarity in vertebrate limb outgrowthJeffery Barrow; Fiscal Year: 2007..Furthermore he will examine the epistatic relationship between Fgf and Wnt/PCP signaling in regulating cell polarity. [unreadable] [unreadable] [unreadable]..
- The role of s-SHIP in normal and tumorigenic development of the skinLARRY ROHRSCHNEIDER; Fiscal Year: 2007..Relevance: The research in this proposal is aimed at understanding the expression and function of a single protein which may be critical for the development of the epidermis. [unreadable] [unreadable] [unreadable]..