Genomes and Genes
Gene Symbol: Rbfox3
Description: RNA binding protein, fox-1 homolog (C. elegans) 3
Alias: Fox-3, Hrnbp3, NeuN, Neuna60, RNA binding protein fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclear antigen A60, neuronal nuclei antigen
- Tang Z, Zheng S, Nikolic J, Black D. Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteins. Mol Cell Biol. 2009;29:4757-65 pubmed publisher..These results demonstrate that the Fox protein family is playing a key role in tuning the properties of CaV1.2 calcium channels during neuronal development. ..
- Penagarikano O, Abrahams B, Herman E, Winden K, Gdalyahu A, Dong H, et al. Absence of CNTNAP2 leads to epilepsy, neuronal migration abnormalities, and core autism-related deficits. Cell. 2011;147:235-46 pubmed publisher..These data demonstrate a functional role for CNTNAP2 in brain development and provide a new tool for mechanistic and therapeutic research in ASD. ..
- Kim K, Kim Y, Adelstein R, Kawamoto S. Fox-3 and PSF interact to activate neural cell-specific alternative splicing. Nucleic Acids Res. 2011;39:3064-78 pubmed publisherFox-1 family (Fox) proteins, which consist of Fox-1 (A2BP1), Fox-2 (Rbm9) and Fox-3 (NeuN) in mammals, bind to the RNA element UGCAUG and regulate alternative pre-mRNA splicing...
- Kim K, Adelstein R, Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J Biol Chem. 2009;284:31052-61 pubmed publisherb>NeuN (neuronal nuclei) is a neuron-specific nuclear protein which is identified by immunoreactivity with a monoclonal antibody, anti-NeuN...
- Kim K, Nam J, Mukouyama Y, Kawamoto S. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol. 2013;200:443-58 pubmed publisher..Here, we focus on a neuron-specific RNA-binding protein, Rbfox3, recently identified as the antigen of the widely used anti-NeuN antibody...
- Ashrafi S, Lalancette Hébert M, Friese A, Sigrist M, Arber S, Shneider N, et al. Wnt7A identifies embryonic γ-motor neurons and reveals early postnatal dependence of γ-motor neurons on a muscle spindle-derived signal. J Neurosci. 2012;32:8725-31 pubmed publisher..Furthermore, using Wnt7a expression as an early marker of γ-MN identity, we demonstrate a previously unknown dependence of γ-MNs on a muscle spindle-derived, GDNF-independent signal during the first postnatal week. ..
- Lorenz A, Deutschmann M, Ahlfeld J, Prix C, Koch A, Smits R, et al. Severe alterations of cerebellar cortical development after constitutive activation of Wnt signaling in granule neuron precursors. Mol Cell Biol. 2011;31:3326-38 pubmed publisher..In summary, we conclude that cerebellar granule neurons essentially require appropriate levels of Wnt signaling to balance their proliferation and differentiation. ..
- Hori K, Cholewa Waclaw J, Nakada Y, Glasgow S, Masui T, Henke R, et al. A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling. Genes Dev. 2008;22:166-78 pubmed publisher....
- Schultz K, Banisadr G, Lastra R, McGuire T, Kessler J, Miller R, et al. Geminin-deficient neural stem cells exhibit normal cell division and normal neurogenesis. PLoS ONE. 2011;6:e17736 pubmed publisher..Both the growth rate and the cell cycle distribution of cultured Gmnn(Δ/Δ) neurosphere cells are indistinguishable from controls. We conclude that Geminin is largely dispensable for most of embryonic and adult mammalian neurogenesis. ..
- Galichet C, Guillemot F, Parras C. Neurogenin 2 has an essential role in development of the dentate gyrus. Development. 2008;135:2031-41 pubmed publisher..These data establish a unique role of Ngn2 in DG neurogenesis during development and raise the possibility that Ngn2 has a similar function in adult neurogenesis. ..
- Liu C, Maejima T, Wyler S, Casadesus G, Herlitze S, Deneris E. Pet-1 is required across different stages of life to regulate serotonergic function. Nat Neurosci. 2010;13:1190-8 pubmed publisher..These findings indicate that Pet-1 is required across the lifespan of the mouse and that behavioral pathogenesis can result from both developmental and adult-onset alterations in serotonergic transcription. ..
- Kuwabara T, Hsieh J, Muotri A, Yeo G, Warashina M, Lie D, et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci. 2009;12:1097-105 pubmed publisher....
- Goebbels S, Bormuth I, Bode U, Hermanson O, Schwab M, Nave K. Genetic targeting of principal neurons in neocortex and hippocampus of NEX-Cre mice. Genesis. 2006;44:611-21 pubmed..The NEX-Cre mouse will be a valuable tool for behavioral research and the conditional inactivation of target genes in pyramidal neurons of the dorsal telencephalon. ..
- Sudarov A, Joyner A. Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers. Neural Dev. 2007;2:26 pubmed....
- Englund C, Fink A, Lau C, Pham D, Daza R, Bulfone A, et al. Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex. J Neurosci. 2005;25:247-51 pubmed....
- Wang V, Rose M, Zoghbi H. Math1 expression redefines the rhombic lip derivatives and reveals novel lineages within the brainstem and cerebellum. Neuron. 2005;48:31-43 pubmed....
- Koo B, Yoon M, Yoon K, Im S, Kim Y, Kim C, et al. An obligatory role of mind bomb-1 in notch signaling of mammalian development. PLoS ONE. 2007;2:e1221 pubmed..Our data provide the first evidence that Mib1 is essential for Jagged as well as Deltalike ligand-mediated Notch signaling in mammalian development, while Neur1, Neur2, and Mib2 are dispensable. ..
- Hand R, Bortone D, Mattar P, Nguyen L, Heng J, Guerrier S, et al. Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex. Neuron. 2005;48:45-62 pubmed....
- Massa F, Koehl M, Koelh M, Wiesner T, Grosjean N, Revest J, et al. Conditional reduction of adult neurogenesis impairs bidirectional hippocampal synaptic plasticity. Proc Natl Acad Sci U S A. 2011;108:6644-9 pubmed publisher....
- Way S, McKenna J, Mietzsch U, Reith R, Wu H, Gambello M. Loss of Tsc2 in radial glia models the brain pathology of tuberous sclerosis complex in the mouse. Hum Mol Genet. 2009;18:1252-65 pubmed publisher....
- Niewmierzycka A, Mills J, St Arnaud R, Dedhar S, Reichardt L. Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly. J Neurosci. 2005;25:7022-31 pubmed..This study demonstrates a critical role for Ilk in cortical lamination and suggests that Ilk-associated pathways are involved in the pathogenesis of cobblestone lissencephalies. ..
- Lind D, Franken S, Kappler J, Jankowski J, Schilling K. Characterization of the neuronal marker NeuN as a multiply phosphorylated antigen with discrete subcellular localization. J Neurosci Res. 2005;79:295-302 pubmedb>NeuN (neuronal nuclei) is an antigen used widely in research and diagnostics to identify postmitotic neurons...
- Lavado A, Oliver G. Prox1 expression patterns in the developing and adult murine brain. Dev Dyn. 2007;236:518-24 pubmed..In adulthood, Prox1 expression remains only in the hippocampus and cerebellum. These complex patterns of expression suggest that Prox1 activity is differentially required during brain development and adulthood. ..
- Ye F, Chen Y, Hoang T, Montgomery R, Zhao X, Bu H, et al. HDAC1 and HDAC2 regulate oligodendrocyte differentiation by disrupting the beta-catenin-TCF interaction. Nat Neurosci. 2009;12:829-38 pubmed publisher..Thus, crosstalk between HDAC1/2 and the canonical Wnt signaling pathway mediated by TCF7L2 serves as a regulatory mechanism for oligodendrocyte differentiation. ..
- Hoffmann S, Hos D, Küspert M, Lang R, Lovell Badge R, Wegner M, et al. Stem cell factor Sox2 and its close relative Sox3 have differentiation functions in oligodendrocytes. Development. 2014;141:39-50 pubmed publisher..This study presents one of the few cases in which SoxB1 proteins, including the stem cell factor Sox2, are associated with differentiation rather than precursor functions. ..
- Dredge B, Jensen K. NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2. PLoS ONE. 2011;6:e21585 pubmed publisher..and mass spectrometry of the two major NeuN species at 45-50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the ..
- Yano M, Hayakawa Yano Y, Mele A, Darnell R. Nova2 regulates neuronal migration through an RNA switch in disabled-1 signaling. Neuron. 2010;66:848-58 pubmed publisher..Thus, Nova2 regulates an RNA switch controlling the ability of Dab1 to mediate neuronal responsiveness to reelin signaling and neuronal migration, suggesting new links between splicing regulation, brain disease, and development. ..
- Rasin M, Gazula V, Breunig J, Kwan K, Johnson M, Liu Chen S, et al. Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. Nat Neurosci. 2007;10:819-27 pubmed..Thus, by regulating RGC adhesion and polarity, Numb and Numbl are required for the tissue architecture of neurogenic niches and the cerebral cortex. ..
- Underwood J, Boutz P, Dougherty J, Stoilov P, Black D. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol Cell Biol. 2005;25:10005-16 pubmed..They do this through a splicing enhancer function, in addition to their apparent negative effects on splicing in vertebrate muscle and in worms. ..
- Zhou B, Zhu Y, Lin L, Cai Q, Sheng Z. Snapin deficiency is associated with developmental defects of the central nervous system. Biosci Rep. 2011;31:151-8 pubmed publisher..In addition, our study supports the existence of a functional interplay between the autophagy-lysosome and ubiquitin-proteasome systems in the protein quality-control process. ..
- Hochstim C, Deneen B, Lukaszewicz A, Zhou Q, Anderson D. Identification of positionally distinct astrocyte subtypes whose identities are specified by a homeodomain code. Cell. 2008;133:510-22 pubmed publisher....
- Kawase Koga Y, Otaegi G, Sun T. Different timings of Dicer deletion affect neurogenesis and gliogenesis in the developing mouse central nervous system. Dev Dyn. 2009;238:2800-12 pubmed publisher..Our studies of different timings of Dicer deletion demonstrate the importance of the Dicer-mediated microRNA pathway in regulating distinct phases of neurogenesis and gliogenesis during the CNS development. ..
- Taylor M, Yeager K, Morrison S. Physiological Notch signaling promotes gliogenesis in the developing peripheral and central nervous systems. Development. 2007;134:2435-47 pubmed..These results demonstrate that physiological Notch signaling is required for gliogenesis in vivo, independent of the role of Notch in the maintenance of undifferentiated neural progenitors. ..
- Camarero G, Tyrsin O, Xiang C, Pfeiffer V, Pleiser S, Wiese S, et al. Cortical migration defects in mice expressing A-RAF from the B-RAF locus. Mol Cell Biol. 2006;26:7103-15 pubmed..Our data reveal that B-RAF is an important mediator of neuronal survival, migration, and dendrite formation and that A-RAF cannot fully compensate for these functions. ..
- Pawlisz A, Feng Y. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes. PLoS Biol. 2011;9:e1001172 pubmed publisher....
- Yu Y, Chen Y, Kim B, Wang H, Zhao C, He X, et al. Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. Cell. 2013;152:248-61 pubmed publisher....
- Stolt C, Rehberg S, Ader M, Lommes P, Riethmacher D, Schachner M, et al. Terminal differentiation of myelin-forming oligodendrocytes depends on the transcription factor Sox10. Genes Dev. 2002;16:165-70 pubmed..Sox10 directly regulates myelin gene expression in oligodendrocytes, but does not control erbB3 expression as in peripheral glia. Sox10 thus functions in peripheral and central glia at different stages and through different mechanisms. ..
- Stolt C, Lommes P, Friedrich R, Wegner M. Transcription factors Sox8 and Sox10 perform non-equivalent roles during oligodendrocyte development despite functional redundancy. Development. 2004;131:2349-58 pubmed..Resulting differences in protein amounts might be a main reason for the weaker impact of Sox8 on oligodendrocyte development and for unidirectional compensation of the Sox8 loss by Sox10. ..
- Mullen R, Buck C, Smith A. NeuN, a neuronal specific nuclear protein in vertebrates. Development. 1992;116:201-11 pubmed..of these, mAb A60, recognizes a vertebrate nervous system- and neuron-specific nuclear protein that we have named NeuN (Neuronal Nuclei)...
- Theriault F, Roy P, Stifani S. AML1/Runx1 is important for the development of hindbrain cholinergic branchiovisceral motor neurons and selected cranial sensory neurons. Proc Natl Acad Sci U S A. 2004;101:10343-8 pubmed..Runx1 inactivation also leads to a loss of selected sensory neurons in trigeminal and vestibulocochlear ganglia. These findings uncover previously unrecognized roles for Runx1 in the regulation of mammalian neuronal subtype development. ..
- Nieto M, Monuki E, Tang H, Imitola J, Haubst N, Khoury S, et al. Expression of Cux-1 and Cux-2 in the subventricular zone and upper layers II-IV of the cerebral cortex. J Comp Neurol. 2004;479:168-80 pubmed..The patterns of expression of Cux genes suggest potential roles as determinants of the neuronal fate of the upper cortical layer neurons. ..
- Liu R, Cai J, Hu X, Tan M, Qi Y, German M, et al. Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor. Development. 2003;130:6221-31 pubmed..In the hindbrain, unlike in the spinal cord, Olig1 and Olig2 can be expressed both inside and outside the Nkx6.1-expressing domains and oligodendrogenesis in this region is not dependent on Nkx6.1 activity. ..
- Corbo J, Deuel T, Long J, LaPorte P, Tsai E, Wynshaw Boris A, et al. Doublecortin is required in mice for lamination of the hippocampus but not the neocortex. J Neurosci. 2002;22:7548-57 pubmed..Behavioral tests show defects in context and cued conditioned fear tests, suggesting that deficits in hippocampal learning accompany the abnormal cytoarchitecture. ..
- Stolt C, Lommes P, Sock E, Chaboissier M, Schedl A, Wegner M. The Sox9 transcription factor determines glial fate choice in the developing spinal cord. Genes Dev. 2003;17:1677-89 pubmed....
- Corrales J, Rocco G, Blaess S, Guo Q, Joyner A. Spatial pattern of sonic hedgehog signaling through Gli genes during cerebellum development. Development. 2004;131:5581-90 pubmed..Taken together, these studies demonstrate that positive Shh signaling through Gli2 is required to generate a sufficient number of GCPs for proper lobe growth. ..
- Shu T, Li Y, Keller A, Richards L. The glial sling is a migratory population of developing neurons. Development. 2003;130:2929-37 pubmed..These data indicate that the sling may be a source of, or migratory pathway for, developing neurons in the rostral forebrain, suggesting additional functions for the sling independent of callosal axon guidance. ..
- Hendricks T, Fyodorov D, Wegman L, Lelutiu N, Pehek E, Yamamoto B, et al. Pet-1 ETS gene plays a critical role in 5-HT neuron development and is required for normal anxiety-like and aggressive behavior. Neuron. 2003;37:233-47 pubmed..These findings indicate that Pet-1 is a critical determinant of 5-HT neuron identity and implicate a Pet-1-dependent program in serotonergic modulation of behavior. ..
- Shu T, Butz K, Plachez C, Gronostajski R, Richards L. Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice. J Neurosci. 2003;23:203-12 pubmed..2001) appear relatively normal. These results support an essential role for midline glia in callosum development and a role for Nfia in the formation of midline glial structures. ..
- Ashique A, Kharazia V, Yaka R, Phamluong K, Peterson A, Ron D. Localization of the scaffolding protein RACK1 in the developing and adult mouse brain. Brain Res. 2006;1069:31-8 pubmed..Our results suggest an important role for RACK1 during CNS development and support multiple functions of the protein in the adult brain. ..
- Mukouyama Y, Deneen B, Lukaszewicz A, Novitch B, Wichterle H, Jessell T, et al. Olig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo. Proc Natl Acad Sci U S A. 2006;103:1551-6 pubmed..These results do not support the commonly held view that most neuroepithelial cells in the embryonic CNS VZ are stem cells in vivo. ..
- Lizen B, Hutlet B, Bissen D, Sauvegarde D, Hermant M, Ahn M, et al. HOXA5 localization in postnatal and adult mouse brain is suggestive of regulatory roles in postmitotic neurons. J Comp Neurol. 2017;525:1155-1175 pubmed publisher..J. Comp. Neurol. 525:1155-1175, 2017. © 2016 Wiley Periodicals, Inc. ..
- Vasiljevic M, Heisler F, Hausrat T, Fehr S, Milenkovic I, Kneussel M, et al. Spatio-temporal expression analysis of the calcium-binding protein calumenin in the rodent brain. Neuroscience. 2012;202:29-41 pubmed publisher..This study points to a possible important role of calumenin in migration and differentiation of neurons, and/or in Ca(2+) signaling between glial cells and neurons. ..
- Felfly H, Xue J, Zambon A, Muotri A, Zhou D, Haddad G. Identification of a neuronal gene expression signature: role of cell cycle arrest in murine neuronal differentiation in vitro. Am J Physiol Regul Integr Comp Physiol. 2011;301:R727-45 pubmed publisher....
- Holstege J, de Graaff W, Hossaini M, Cardona Cano S, Jaarsma D, van den Akker E, et al. Loss of Hoxb8 alters spinal dorsal laminae and sensory responses in mice. Proc Natl Acad Sci U S A. 2008;105:6338-43 pubmed publisher..The data therefore suggest that a lower number of neurons in the upper spinal laminae and neuronal disorganization in the dorsal horn underlie the sensory defects including the excessive grooming of the Hoxb8 mutant. ..
- Cisternas C, Tome K, Caeiro X, Dadam F, Garcia Segura L, Cambiasso M. Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain. Mol Cell Endocrinol. 2015;414:99-110 pubmed publisher..In conclusion, sex chromosomes determine sex dimorphisms in aromatase expression and regulation in the developing mouse brain. ..
- Jakovcevski M, Ruan H, Shen E, Dincer A, Javidfar B, Ma Q, et al. Neuronal Kmt2a/Mll1 histone methyltransferase is essential for prefrontal synaptic plasticity and working memory. J Neurosci. 2015;35:5097-108 pubmed publisher..Therefore, mature prefrontal neurons critically depend on maintenance of Mll1-regulated H3K4 methylation at a subset of genes with an essential role in cognition and emotion. ..
- Chatoo W, Abdouh M, David J, Champagne M, Ferreira J, Rodier F, et al. The polycomb group gene Bmi1 regulates antioxidant defenses in neurons by repressing p53 pro-oxidant activity. J Neurosci. 2009;29:529-42 pubmed publisher..These findings provide a molecular mechanism explaining how Bmi1 regulates free radical concentrations and reveal the biological impact of Bmi1 deficiency on neuronal survival and aging. ..
- Sosanya N, Huang P, Cacheaux L, Chen C, Nguyen K, Perrone Bizzozero N, et al. Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1. J Cell Biol. 2013;202:53-69 pubmed publisher..1 mRNA. Hence, mTORC1 activity regulation of mRNA stability and high affinity HuD-target mRNA degradation mediates the bidirectional expression of dendritic Kv1.1 ion channels. ..
- Dang E, Barbi J, Yang H, Jinasena D, Yu H, Zheng Y, et al. Control of T(H)17/T(reg) balance by hypoxia-inducible factor 1. Cell. 2011;146:772-84 pubmed publisher..These findings highlight the importance of metabolic cues in T cell fate determination and suggest that metabolic modulation could ameliorate certain T cell-based immune pathologies. ..
- Nishide K, Nakatani Y, Kiyonari H, Kondo T. Glioblastoma formation from cell population depleted of Prominin1-expressing cells. PLoS ONE. 2009;4:e6869 pubmed publisher..We show here that the tamoxifen-treated GICs-LD (GICs-DTA) form tumor-spheres in culture and transplantable GBM in vivo. Thus, our studies demonstrate that Prom1-expressing cells are dispensable for gliomagenesis in this mouse model. ..
- Schuster Gossler K, Cordes R, Muller J, Geffers I, Delany Heiken P, Taft M, et al. Context-Dependent Sensitivity to Mutations Disrupting the Structural Integrity of Individual EGF Repeats in the Mouse Notch Ligand DLL1. Genetics. 2016;202:1119-33 pubmed publisher..In conclusion, the structural integrity of each individual EGF repeat in the extracellular domain of DLL1 is necessary for full DLL1 activity, and certain mutations in Dll1 might contribute to spondylocostal dysostosis in humans. ..
- Barford K, Yap C, Dwyer N, Winckler B. The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo. J Comp Neurol. 2017;525:1861-1878 pubmed publisher..This divergent and restricted expression likely reflects differential needs for this class of trafficking regulators in different neurons during different stages of maturation. ..