RIG I

Summary

Gene Symbol: RIG I
Description: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Alias: 6430573D20Rik, C330021E21, RIG-I, RLR-1, DEAD box protein 58, DEAD/H box polypeptide RIG-I, RIG-1, RIG-I-like receptor 1, retinoic acid-inducible gene 1 protein, retinoic acid-inducible gene I protein, retinoic acid-inducible gene-I
Species: mouse

Top Publications

  1. ncbi Rig-I-/- mice develop colitis associated with downregulation of G alpha i2
    Yi Wang
    Department of Medical Genetics, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    Cell Res 17:858-68. 2007
  2. pmc RIG-like helicase innate immunity inhibits vascular endothelial growth factor tissue responses via a type I IFN-dependent mechanism
    Bing Ma
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA
    Am J Respir Crit Care Med 183:1322-35. 2011
  3. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
  4. pmc Species-specific inhibition of RIG-I ubiquitination and IFN induction by the influenza A virus NS1 protein
    Ricardo Rajsbaum
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 8:e1003059. 2012
  5. pmc LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010
  6. pmc Identification of host cytosolic sensors and bacterial factors regulating the type I interferon response to Legionella pneumophila
    Kathryn M Monroe
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    PLoS Pathog 5:e1000665. 2009
  7. pmc RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
    Andrea Ablasser
    Institute for Clinical Chemistry and Pharmacology, University of Bonn, Bonn, Germany
    Nat Immunol 10:1065-72. 2009
  8. pmc Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5
    Hiroki Kato
    Laboratory of Host Defense, World Premiere International Immunology Frontier Research Center, Osaka University, Suita, Osaka 565 0871, Japan
    J Exp Med 205:1601-10. 2008
  9. pmc Innate immunity induced by composition-dependent RIG-I recognition of hepatitis C virus RNA
    Takeshi Saito
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington 98195 7650, USA
    Nature 454:523-7. 2008
  10. pmc Establishment and maintenance of the innate antiviral response to West Nile Virus involves both RIG-I and MDA5 signaling through IPS-1
    Brenda L Fredericksen
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA
    J Virol 82:609-16. 2008

Research Grants

  1. Innate Intracellular Response to West Nile Virus
    Brenda Fredericksen; Fiscal Year: 2007

Scientific Experts

  • Michaela Ulrike Gack
  • Hiroyuki Oshiumi
  • Brenda Fredericksen
  • Shizuo Akira
  • Hiroki Kato
  • Gunther Hartmann
  • Michael Gale
  • Osamu Takeuchi
  • Hendrik Poeck
  • Stefan Endres
  • Hong Xin Zhang
  • Zhu Chen
  • Takashi Fujita
  • Jiang Zhu
  • Mitsutoshi Yoneyama
  • Sai Juan Chen
  • Katherine A Fitzgerald
  • Zhu Gang Wang
  • Winfried Barchet
  • Simon Rothenfusser
  • Xian Yang Li
  • Jin Hou
  • Adolfo Garcia-Sastre
  • Lin Jia Jiang
  • Mehul S Suthar
  • Yue Ping Sun
  • Veit Hornung
  • Tatsuya Kozaki
  • Yueh Ming Loo
  • Nan Li
  • Keqin Yan
  • Weiwei Zhu
  • May K Wang
  • Shintaro Sato
  • Ken J Ishii
  • Filiz Civril
  • Yoshiharu Matsuura
  • Wu Zhang
  • Ulrich Kalinke
  • Carole Bourquin
  • Takashi Satoh
  • Li Lin
  • Masahiro Yamamoto
  • Jurgen Ruland
  • David Anz
  • Nan Nan Zhang
  • Tohru Tsujimura
  • Christian Hotz
  • Xiaoxu Fan
  • R F Foronjy
  • Kathleen Pestal
  • Sang Hyeon Yeon
  • Taro Kawai
  • Tatsuya Saitoh
  • Takuma Misawa
  • Yihui Fan
  • Shengyong Yang
  • Hao Sen Chiang
  • Peter Liehl
  • Michihiro Takahama
  • Zongmin Du
  • Satoshi Uematsu
  • Akshay A D'Cruz
  • Minoru Fukuda
  • Hsin Lin Lu
  • Xiaoyan Zhang
  • Iris K Pang
  • Qiaoyuan Chen
  • Hanna Lee
  • Yongmei Chen
  • Effi Wies
  • Jonathan Ellermeier
  • Megan E Ermler
  • Yushi Hayashi
  • Lili Yu
  • John S Errett
  • Daishu Han
  • Natalya P Maharaj
  • Jae U Jung
  • Lei Chen
  • Douglas T Golenbock
  • Jennie Chan
  • Ian C Berke
  • Zeinab Abdullah
  • Puck B van Kasteren
  • Swamy Kumar Polumuri
  • Tobias Asdonk
  • Gretja Schnell
  • Randi K Berg
  • Ryan A Langlois

Detail Information

Publications85

  1. ncbi Rig-I-/- mice develop colitis associated with downregulation of G alpha i2
    Yi Wang
    Department of Medical Genetics, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    Cell Res 17:858-68. 2007
    ..The development of colitis may be, at least in part, associated with downregulation of G alpha i2 and disturbed T-cell homeostasis...
  2. pmc RIG-like helicase innate immunity inhibits vascular endothelial growth factor tissue responses via a type I IFN-dependent mechanism
    Bing Ma
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA
    Am J Respir Crit Care Med 183:1322-35. 2011
    ....
  3. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
    ..These results highlight ingenious mechanisms for initiating antiviral innate immune responses and the action of virus-encoded inhibitors...
  4. pmc Species-specific inhibition of RIG-I ubiquitination and IFN induction by the influenza A virus NS1 protein
    Ricardo Rajsbaum
    Department of Microbiology, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS Pathog 8:e1003059. 2012
    ..In conclusion, our results indicate that influenza NS1 protein targets TRIM25 and Riplet ubiquitin E3 ligases in a species-specific manner for the inhibition of RIG-I ubiquitination and antiviral IFN production...
  5. pmc LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010
    ..Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain...
  6. pmc Identification of host cytosolic sensors and bacterial factors regulating the type I interferon response to Legionella pneumophila
    Kathryn M Monroe
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    PLoS Pathog 5:e1000665. 2009
    ..Thus, our results provide new insights into the molecular mechanisms by which an intracellular bacterial pathogen activates and also represses innate immune responses...
  7. pmc RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
    Andrea Ablasser
    Institute for Clinical Chemistry and Pharmacology, University of Bonn, Bonn, Germany
    Nat Immunol 10:1065-72. 2009
    ..This pathway was important in the sensing of Epstein-Barr virus-encoded small RNAs, which were transcribed by RNA polymerase III and then triggered RIG-I activation. Thus, RNA polymerase III and RIG-I are pivotal in sensing viral DNA...
  8. pmc Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5
    Hiroki Kato
    Laboratory of Host Defense, World Premiere International Immunology Frontier Research Center, Osaka University, Suita, Osaka 565 0871, Japan
    J Exp Med 205:1601-10. 2008
    ..Collectively, RIG-I detects dsRNAs without a 5'-triphosphate end, and RIG-I and MDA5 selectively recognize short and long dsRNAs, respectively...
  9. pmc Innate immunity induced by composition-dependent RIG-I recognition of hepatitis C virus RNA
    Takeshi Saito
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington 98195 7650, USA
    Nature 454:523-7. 2008
    ....
  10. pmc Establishment and maintenance of the innate antiviral response to West Nile Virus involves both RIG-I and MDA5 signaling through IPS-1
    Brenda L Fredericksen
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, USA
    J Virol 82:609-16. 2008
    ..We propose a model in which RIG-I and MDA5 operate cooperatively to establish an antiviral state and mediate an IFN amplification loop that supports immune effector gene expression during WNV infection...
  11. pmc Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity
    Yueh Ming Loo
    Department of Immunology, University of Washington School of Medicine, Box 357650, Seattle, WA 98195 7650, USA
    J Virol 82:335-45. 2008
    ....
  12. ncbi Cell type-specific involvement of RIG-I in antiviral response
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Immunity 23:19-28. 2005
    ..In contrast, plasmacytoid DCs, which produce large amounts of IFN-alpha, use the TLR system rather than RIG-I for viral detection. Taken together, RIG-I and the TLR system exert antiviral responses in a cell type-specific manner...
  13. ncbi Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses
    Thiagarajan Venkataraman
    Department of Microbiology and Immunology and Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, Miami, FL 33136, USA
    J Immunol 178:6444-55. 2007
    ..Collectively, our data indicate a disparate regulatory role for LGP2 in the triggering of innate immune signaling pathways following RNA virus infection...
  14. ncbi The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I
    Simon Rothenfusser
    Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Immunol 175:5260-8. 2005
    ..We propose that Lgp2 acts as a negative feedback regulator of antiviral signaling by sequestering dsRNA from RIG-I...
  15. ncbi A Toll-like receptor-independent antiviral response induced by double-stranded B-form DNA
    Ken J Ishii
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Nat Immunol 7:40-8. 2006
    ..These results suggest that both TBK1 and IKKi are required for innate immune activation by B-DNA, which might be important in antiviral innate immunity and other DNA-associated immune disorders...
  16. ncbi Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Nature 441:101-5. 2006
    ..Together, our data show that RIG-I and MDA5 distinguish different RNA viruses and are critical for host antiviral responses...
  17. pmc IPS-1 is essential for the control of West Nile virus infection and immunity
    Mehul S Suthar
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington, USA
    PLoS Pathog 6:e1000757. 2010
    ..Our data define an innate/adaptive immune interface mediated through IPS-1-dependent RLR signaling that regulates the quantity, quality, and balance of the immune response to WNV infection...
  18. pmc Isoforms of RNA-Editing Enzyme ADAR1 Independently Control Nucleic Acid Sensor MDA5-Driven Autoimmunity and Multi-organ Development
    Kathleen Pestal
    Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Immunity 43:933-44. 2015
    ..Our findings delineate genetically separable roles for both ADAR1 isoforms in vivo, with implications for the human diseases caused by ADAR mutations. ..
  19. pmc RNA helicase signaling is critical for type i interferon production and protection against Rift Valley fever virus during mucosal challenge
    Megan E Ermler
    Center for Global Health and Diseases, Case Western Reserve University, Cleveland, Ohio, USA
    J Virol 87:4846-60. 2013
    ..In addition, we demonstrate that Toll-like-receptor-mediated signaling is not involved in IFN production, further emphasizing the importance of the RNA cellular helicases in type I IFN responses to RVFV...
  20. doi Virus-induced expression of retinoic acid inducible gene-I and melanoma differentiation-associated gene 5 in the cochlear sensory epithelium
    Yushi Hayashi
    Department of Otolaryngology and Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Microbes Infect 15:592-8. 2013
    ..These results strongly suggest that RIG-I and MDA5 participate in innate antiviral responses in cochlear tissue. ..
  21. doi RIG-I-like receptors mediate innate antiviral response in mouse testis
    Weiwei Zhu
    School of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005 China
    Mol Endocrinol 27:1455-67. 2013
    ..We also provide evidence that local injection of poly(I:C) induces antiviral response in the testis of TLR3(-/-) mice. These data provide novel insights into the mechanisms underlying testicular antiviral response. ..
  22. doi Melanoma differentiation-associated gene 5 senses hepatitis B virus and activates innate immune signaling to suppress virus replication
    Hsin Lin Lu
    Institute of Microbiology and Immunology, National Yang Ming University, Taipei 11221, Taiwan
    J Immunol 191:3264-76. 2013
    ..Collectively, our in vitro and in vivo studies both support a critical role for MDA5 in the innate immune response against HBV infection...
  23. pmc GEF-H1 controls microtubule-dependent sensing of nucleic acids for antiviral host defenses
    Hao Sen Chiang
    1 Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA 2
    Nat Immunol 15:63-71. 2014
    ..Microtubule networks sequester GEF-H1 that upon activation is released to enable antiviral signaling by intracellular nucleic acid detection pathways. ..
  24. doi Hepatic RIG-I predicts survival and interferon-α therapeutic response in hepatocellular carcinoma
    Jin Hou
    National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China National Key Laboratory of Medical Molecular Biology and Department of Immunology, Chinese Academy of Medical Sciences, Beijing 100005, China
    Cancer Cell 25:49-63. 2014
    ..Furthermore, we found that RIG-I deficiency promotes HCC carcinogenesis and that hepatic RIG-I expression is lower in men than in women. RIG-I may therefore be a tumor suppressor in HCC and contribute to HCC gender disparity...
  25. pmc Host-cell sensors for Plasmodium activate innate immunity against liver-stage infection
    Peter Liehl
    Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa, Lisboa, Portugal
    Nat Med 20:47-53. 2014
    ..Together, our results show that the liver has sensor mechanisms for Plasmodium that mediate a functional antiparasite response driven by type I IFN. ..
  26. doi RIG-I modulates Src-mediated AKT activation to restrain leukemic stemness
    Xian Yang Li
    State Key Laboratory for Medical Genomics and Shanghai Institute of Hematology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People s Republic of China Shanghai E Institute for Model Organisms, Shanghai 200025, People s Republic of China
    Mol Cell 53:407-19. 2014
    ..Collectively, our data suggest an antileukemia activity of RIG-I via competitively inhibiting Src/AKT association...
  27. pmc USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase
    Yihui Fan
    Texas Children s Cancer Center, Department of Pediatrics, 2 Department of Pathology and Immunology, Dan L Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030
    J Exp Med 211:313-28. 2014
    ..Our studies identify a previously unrecognized role for USP21 in the negative regulation of antiviral response through deubiquitinating RIG-I. ..
  28. pmc Type-I interferons induce lung protease responses following respiratory syncytial virus infection via RIG-I-like receptors
    R F Foronjy
    Department of Medicine, Mount Sinai Roosevelt Hospital, Mount Sinai Health System, Division of Pulmonary and Critical Care Medicine, New York, New York, USA
    Mucosal Immunol 8:161-75. 2015
    ..The significance of RIG-1 protease induction was demonstrated by the fact that inhibiting proteases with batimastat, E64 or ribavirin prevented airway hyperresponsiveness and enhanced viral clearance in RSV-infected mice. ..
  29. doi Transcriptomic response to Yersinia pestis: RIG-I like receptor signaling response is detrimental to the host against plague
    Zongmin Du
    State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China Electronic address
    J Genet Genomics 41:379-96. 2014
    ..Moreover, infection of pathogenic bacteria other than Y. pestis also altered the expression of the RLR pathway, suggesting that the response of RLR pathway to bacterial infection is a universal mechanism. ..
  30. pmc Phosphatidylinositol-3-kinase and Akt are required for RIG-I-mediated anti-viral signalling through cross-talk with IPS-1
    Sang Hyeon Yeon
    College of Pharmacy, Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon, Korea
    Immunology 144:312-20. 2015
    ..The results describe a novel, interactive relationship between RIG-I downstream signalling molecules resulting in efficient anti-viral immunity. ..
  31. pmc Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs
    Shengyong Yang
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213
    J Immunol 193:3436-45. 2014
    ..These results explain why loss of ADARA1 causes IFN induction and also indicates a mechanism for the involvement of ADAR1 in autoimmune diseases such as Aicardi-Goutières syndrome. ..
  32. pmc Role of zinc-finger anti-viral protein in host defense against Sindbis virus
    Tatsuya Kozaki
    Laboratory of Host Defense, World Premier International Immunology Frontier Research Center, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Int Immunol 27:357-64. 2015
    ..Therefore, ZAP is an RNA-sensing anti-viral effector molecule that mediates the type-I-IFN-dependent host defense against SINV. ..
  33. pmc RIG-I-dependent antiviral immunity is effective against an RNA virus encoding a potent suppressor of RNAi
    Xiaoxu Fan
    Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China Department of Plant Pathology and Microbiology, and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Biochem Biophys Res Commun 460:1035-40. 2015
    ..Our findings suggest that RIG-I directs a typical IFN-dependent antiviral response against an RNA virus capable of suppressing the RNAi response. ..
  34. doi TLR and RLR Signaling Are Reprogrammed in Opposite Directions after Detection of Viral Infection
    Christian Hotz
    Department of Medicine, Faculty of Science, University of Fribourg, 1700 Fribourg, Switzerland Division of Clinical Pharmacology, Center for Integrated Protein Science Munich, Ludwig Maximilian University Munich, 80336 Munich, Germany and
    J Immunol 195:4387-95. 2015
    ..These dynamic adaptations to infection may reinforce antiviral immunity and at the same time serve to limit pathological inflammation. ..
  35. pmc Crystal structure of the TRIM25 B30.2 (PRYSPRY) domain: a key component of antiviral signalling
    Akshay A D'Cruz
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, U S A
    Biochem J 456:231-40. 2013
    ..This was supported by mutagenesis and functional analyses, which identified two key residues (Asp(488) and Trp(621)) in the TRIM25 B30.2 domain as being critical for binding to the RIG-I CARDs...
  36. pmc The essential, nonredundant roles of RIG-I and MDA5 in detecting and controlling West Nile virus infection
    John S Errett
    Department of Immunology, University of Washington School of Medicine, Seattle, Washington, USA
    J Virol 87:11416-25. 2013
    ..Collectively, these experiments highlight the necessity and function of multiple related, cytoplasmic host sensors in orchestrating an effective immune response against an acute viral infection. ..
  37. pmc Efficient influenza A virus replication in the respiratory tract requires signals from TLR7 and RIG-I
    Iris K Pang
    Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 110:13910-5. 2013
    ..Our data suggest that IAV uses physiological levels of inflammatory responses for its replicative advantage and highlight the complex interplay between viruses and the host innate-immune responses. ..
  38. pmc Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus
    Hanna Lee
    Laboratory of Host Defense, World Premier International Immunology Frontier Research Center, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 110:12379-84. 2013
    ..Although the retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) RIG-I and melanoma differentiation-associated protein 5 detect various ..
  39. doi Toll-like receptor 3 and RIG-I-like receptor activation induces innate antiviral responses in mouse ovarian granulosa cells
    Keqin Yan
    Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, PR China
    Mol Cell Endocrinol 372:73-85. 2013
    ..Data suggest that TLR3, MDA5 and RIG-I cooperate in mediating innate antiviral responses in granulosa cells, which may contribute to the defense of the ovary against viral infections...
  40. doi Expression and functional characterization of retinoic acid-inducible gene-I-like receptors of mast cells in response to viral infection
    Minoru Fukuda
    Department of Infection Control Science, Juntendo University School of Medicine, Tokyo, Japan
    J Innate Immun 5:163-73. 2013
    ..These data provide additional novel information that improves our understanding of antiviral innate responses that involve mast cells...
  41. pmc Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists
    Swamy Kumar Polumuri
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 189:50-60. 2012
    ..Together, these studies greatly extend what is currently known about the regulation of IL-33 induction in macrophages stimulated by bacterial and viral agonists that engage distinct innate immune signaling pathways...
  42. pmc Rabies virus infection induces type I interferon production in an IPS-1 dependent manner while dendritic cell activation relies on IFNAR signaling
    Elizabeth J Faul
    Department of Microbiology and Immunology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
    PLoS Pathog 6:e1001016. 2010
    ..In order to determine the role that IPS-1 plays in vivo, we infected mice with pathogenic RABV. We see that IPS-1-/- mice are more susceptible to infection than IPS-1+/+ mice and have a significantly increased incident of limb paralysis...
  43. pmc LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection
    Ying Li
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    Proc Natl Acad Sci U S A 109:11770-5. 2012
    ..These findings suggest that LSm14A is a sensor for both viral RNA and DNA and plays an important role in initiating IFN-β induction in the early phase of viral infection...
  44. pmc Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses
    Ryan A Langlois
    Department of Microbiology and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 109:12117-22. 2012
    ..Taking these data together, this research describes a unique platform to study the host response to IAV and provides insights into the mechanism of antigen presentation and the induction of IFN-I...
  45. pmc 5'-Triphosphate-short interfering RNA: potent inhibition of influenza A virus infection by gene silencing and RIG-I activation
    Li Lin
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Virol 86:10359-69. 2012
    ..The development of such dual functional RNA molecules will greatly contribute to the arsenal of tools to combat not only influenza viruses but also other important viral pathogens...
  46. pmc IFNγ inhibits the cytosolic replication of Shigella flexneri via the cytoplasmic RNA sensor RIG-I
    Stephanie P Jehl
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 8:e1002809. 2012
    ..Additionally, these findings expand our understanding of how IFNγ recognizes, and ultimately restricts, bacterial pathogens within host cells...
  47. pmc Uridine composition of the poly-U/UC tract of HCV RNA defines non-self recognition by RIG-I
    Gretja Schnell
    Department of Immunology, University of Washington, School of Medicine, Seattle, Washington, United States of America
    PLoS Pathog 8:e1002839. 2012
    ..Our studies define a basis of non-self discrimination by RIG-I and offer insights into the antiviral therapeutic potential of targeted RIG-I signaling activation...
  48. pmc RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids
    Zeinab Abdullah
    Institutes of Molecular Medicine and Experimental Immunology, Friedrich Wilhelms Universitat Bonn, Bonn, Germany
    EMBO J 31:4153-64. 2012
    ..In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG-I provides a mechanistic explanation for efficient induction of immunity by live bacteria...
  49. doi Therapeutic efficacy of bifunctional siRNA combining TGF-β1 silencing with RIG-I activation in pancreatic cancer
    Jonathan Ellermeier
    Medizinische Klinik und Poliklinik IV, Klinikum der Universitat Munchen, Germany
    Cancer Res 73:1709-20. 2013
    ..In conclusion, combing TGF-β gene silencing with RIG-I signaling confers potent antitumor efficacy against pancreatic cancer by breaking tumor-induced CD8(+) T cell suppression...
  50. pmc Dephosphorylation of the RNA sensors RIG-I and MDA5 by the phosphatase PP1 is essential for innate immune signaling
    Effi Wies
    Microbiology Division, New England Primate Research Center, Harvard Medical School, 1 Pine Hill Drive, Southborough, MA 01772 9102, USA
    Immunity 38:437-49. 2013
    ..This work identifies PP1α and PP1γ as regulators of antiviral innate immune responses to various RNA viruses, including influenza virus, paramyxovirus, dengue virus, and picornavirus...
  51. pmc Rig-I regulates NF-κB activity through binding to Nf-κb1 3'-UTR mRNA
    Hong Xin Zhang
    State Key Laboratory of Medical Genomics, Research Center for Experimental Medicine, Shanghai Institute of Hematology, Rui Jin Hospital Shanghai Jiao Tong University School of Medicine, SJTUSM, Shanghai 200025, China
    Proc Natl Acad Sci U S A 110:6459-64. 2013
    b>Retinoic acid inducible gene I (RIG-I) senses viral RNAs and triggers innate antiviral responses through induction of type I IFNs and inflammatory cytokines. However, whether RIG-I interacts with host cellular RNA remains undetermined...
  52. doi Recognition of RNA virus by RIG-I results in activation of CARD9 and inflammasome signaling for interleukin 1 beta production
    Hendrik Poeck
    III Medizinische Klinik, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Nat Immunol 11:63-9. 2010
    ..Our results identify the CARD9-Bcl-10 module as an essential component of the RIG-I-dependent proinflammatory response and establish RIG-I as a sensor able to activate the inflammasome in response to certain RNA viruses...
  53. pmc MDA5 cooperatively forms dimers and ATP-sensitive filaments upon binding double-stranded RNA
    Ian C Berke
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
    EMBO J 31:1714-26. 2012
    ..We propose a signalling model in which the CARDs on MDA5-RNA filaments nucleate the assembly of MAVS filaments with the same polymeric geometry...
  54. pmc DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells
    Zhiqiang Zhang
    Department of Immunology, Center for Cancer Immunology Research, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Immunity 34:866-78. 2011
    ..This sensor was localized within the cytosol, independent of the endosomes. Thus, the DDX1-DDX21-DHX36 complex represents a dsRNA sensor that uses the TRIF pathway to activate type I IFN responses in the cytosol of mDCs...
  55. doi Endothelial RIG-I activation impairs endothelial function
    Tobias Asdonk
    Department of Medicine Cardiology, University of Bonn, Sigmund Freud Str 25, 53105 Bonn, Germany
    Biochem Biophys Res Commun 420:66-71. 2012
    ..Here, we analyzed the impact of endothelial retinoic acid inducible gene I (RIG-I) activation upon vascular endothelial biology.
  56. pmc The RIG-I ATPase domain structure reveals insights into ATP-dependent antiviral signalling
    Filiz Civril
    Department of Biochemistry at the Gene Center, Ludwig Maximilians University Munich, Feodor Lynen Strasse 25, Munich 81377, Germany
    EMBO Rep 12:1127-34. 2011
    ..Overall, our results indicate that the activation of RIG-I occurs through an RNA- and ATP-driven structural switch in the SF2 domain...
  57. pmc Arterivirus and nairovirus ovarian tumor domain-containing Deubiquitinases target activated RIG-I to control innate immune signaling
    Puck B van Kasteren
    Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    J Virol 86:773-85. 2012
    ....
  58. pmc The toll-like receptor 3-mediated antiviral response is important for protection against poliovirus infection in poliovirus receptor transgenic mice
    Yuko Abe
    Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Virol 86:185-94. 2012
    ..These results suggest that multiple pathways are involved in the antiviral response in mice and that the TLR3-TRIF-mediated signaling pathway plays an essential role in the antiviral response against PV infection...
  59. doi Cytosolic RIG-I-like helicases act as negative regulators of sterile inflammation in the CNS
    Angela Dann
    Department of Neuropathology, University of Freiburg, Freiburg, Germany
    Nat Neurosci 15:98-106. 2011
    ....
  60. pmc Genomic HIV RNA induces innate immune responses through RIG-I-dependent sensing of secondary-structured RNA
    Randi K Berg
    Department of Infectious Diseases, Aarhus University Hospital Skejby, Aarhus, Denmark
    PLoS ONE 7:e29291. 2012
    ..However, knowledge on specific HIV PAMPs and cellular PRRs responsible for inducing innate immune responses remains sparse...
  61. ncbi Retinoic acid-inducible gene-I mediates late phase induction of TNF-alpha by lipopolysaccharide
    Jing Wang
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, China
    J Immunol 180:8011-9. 2008
    ..Thus, RIG-I plays a key role in the expression of TNF-alpha in macrophages in response to LPS stimulation, mainly for the late phase LPS-induced expression of TNF-alpha...
  62. pmc The Atg5 Atg12 conjugate associates with innate antiviral immune responses
    Nao Jounai
    Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Proc Natl Acad Sci U S A 104:14050-5. 2007
    ..Thus, in contrast to its role in promoting the bactericidal process, a component of the autophagic machinery appears to block innate antiviral immune responses, thereby contributing to RNA virus replication in host cells...
  63. pmc Respiratory syncytial virus induces RelA release from cytoplasmic 100-kDa NF-kappa B2 complexes via a novel retinoic acid-inducible gene-I{middle dot}NF- kappa B-inducing kinase signaling pathway
    Ping Liu
    Department of Medicine, University of Texas Medical Branch, Galveston, TX 77555 1060, USA
    J Biol Chem 283:23169-78. 2008
    ..IKKalpha complex downstream of RIG-I.MAVS. This pathway may be a potential target for RSV treatment...
  64. pmc RIG-I plays a critical role in negatively regulating granulocytic proliferation
    Nan Nan Zhang
    State Key Laboratory for Medical Genomics, Institute of Health Science, Shanghai Institute for Biological Sciences and Graduate School, Chinese Academy of Sciences, People s Republic of China
    Proc Natl Acad Sci U S A 105:10553-8. 2008
    ..Thus, our study reveals a critical regulatory role of Rig-I in modulating the generation and differentiation of granulocytes...
  65. doi 5'-Triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma
    Hendrik Poeck
    Institute of Clinical Chemistry and Pharmacology, University Hospital, University of Bonn, Bonn, Germany
    Nat Med 14:1256-63. 2008
    ....
  66. pmc RIG-I and dsRNA-induced IFNbeta activation
    Stephane Hausmann
    Department of Microbiology and Molecular Medicine, University of Geneva Medical School, Geneva, Switzerland
    PLoS ONE 3:e3965. 2008
    ..e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain...
  67. pmc RIG-I-mediated activation of p38 MAPK is essential for viral induction of interferon and activation of dendritic cells: dependence on TRAF2 and TAK1
    Susie S Mikkelsen
    Institute of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000 Aarhus C, Denmark
    J Biol Chem 284:10774-82. 2009
    ..Thus, activation of p38 MAPK by RIG-I proceeds via a TRAF2-TAK1-dependent pathway, where the enzymatic activity of the kinase plays an essential role. The p38 MAPK in turn stimulates important processes in the innate antiviral response...
  68. doi MicroRNA-146a feedback inhibits RIG-I-dependent Type I IFN production in macrophages by targeting TRAF6, IRAK1, and IRAK2
    Jin Hou
    Institute of Immunology, Tsinghua University School of Medicine, Beijing, China
    J Immunol 183:2150-8. 2009
    ..Therefore, we demonstrate that miR-146a, up-regulated during viral infection, is a negative regulator of the RIG-I-dependent antiviral pathway by targeting TRAF6, IRAK1, and IRAK2...
  69. doi An essential role for RIG-I in toll-like receptor-stimulated phagocytosis
    Ling Kong
    The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai JiaoTong University School of Medicine, Shanghai 200025, China
    Cell Host Microbe 6:150-61. 2009
    ..Thus, the regulatory functions of RIG-I are strikingly broad, including a role not only in antiviral responses but in antibacterial responses as well...
  70. doi The viral RNA recognition sensor RIG-I is degraded during encephalomyocarditis virus (EMCV) infection
    Laura Papon
    Universite Montpellier 1, Centre d Etudes d Agents Pathogenes et Biotechnologies pour la Sante CPBS, F 34095 Montpellier, France
    Virology 393:311-8. 2009
    ..Taken together, our data strongly suggest that during evolution RIG-I has been involved for triggering innate immune response to picornavirus infections...
  71. pmc A selective contribution of the RIG-I-like receptor pathway to type I interferon responses activated by cytosolic DNA
    Myoung Kwon Choi
    Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 106:17870-5. 2009
    ..Our study may provide insights into the complexity of host defense mechanisms that thwart immune evasion by DNA-containing pathogens...
  72. pmc Murine coronavirus induces type I interferon in oligodendrocytes through recognition by RIG-I and MDA5
    Jianfeng Li
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W Markham Street, Little Rock, AR 72205, USA
    J Virol 84:6472-82. 2010
    ..These data suggest that activation of the NF-kappaB pathway might not play a critical role in IFN-alpha/beta induction by MHV infection in oligodendrocytes...
  73. doi Amino acids at positions 273 and 394 in rabies virus nucleoprotein are important for both evasion of host RIG-I-mediated antiviral response and pathogenicity
    Tatsunori Masatani
    The United Graduate School of Veterinary Sciences, Gifu University, 1 1 Yanagido, Gifu 501 1193, Japan
    Virus Res 155:168-74. 2011
    ..This correlation reinforces the relation between evasion of host RIG-I-mediated innate immunity and pathogenicity of rabies virus...
  74. pmc RA-inducible gene-I induction augments STAT1 activation to inhibit leukemia cell proliferation
    Lin Jia Jiang
    State Key Laboratory for Medical Genomics, Institute of Health Science, Shanghai Institute for Biological Sciences and Graduate School, Chinese Academy of Sciences, and Shanghai Institute of Hematology, Rui Jin Hospital, Shanghai 200025, People s Republic of China
    Proc Natl Acad Sci U S A 108:1897-902. 2011
    ....
  75. pmc ARF-like protein 16 (ARL16) inhibits RIG-I by binding with its C-terminal domain in a GTP-dependent manner
    Yong Kang Yang
    Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 286:10568-80. 2011
    ..These findings suggested a novel innate immune function for an ARL family member, and a GTP-dependent model in which RIG-I is regulated...
  76. doi Immunostimulatory RNA blocks suppression by regulatory T cells
    David Anz
    Division of Clinical Pharmacology, Center of Integrated Protein Science Munich, University of Munich, Munich, Germany
    J Immunol 184:939-46. 2010
    ..Thus, we show in this study for the first time that activation of a RIG-I-like helicase on Treg cells blocks their suppressive function...
  77. pmc Phosphorylation-mediated negative regulation of RIG-I antiviral activity
    Michaela U Gack
    Department of Microbiology and Molecular Genetics, New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 84:3220-9. 2010
    ..While Thr-170 phosphorylation keeps RIG-I latent, Lys-172 ubiquitination enables RIG-I to form a stable complex with MAVS, thereby inducing IFN signal transduction...
  78. pmc Linear ubiquitin assembly complex negatively regulates RIG-I- and TRIM25-mediated type I interferon induction
    Kyung Soo Inn
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    Mol Cell 41:354-65. 2011
    ....
  79. doi Klotho suppresses RIG-I-mediated senescence-associated inflammation
    Feng Liu
    National Key Laboratory of Protein Engineering and Plant Gene Engineering, LSC, Peking University, Beijing 100871, China
    Nat Cell Biol 13:254-62. 2011
    ..Our study uncovers a mechanism in which klotho functions as an anti-ageing factor through the suppression of RIG-I-mediated inflammation...
  80. pmc Immune surveillance by mast cells during dengue infection promotes natural killer (NK) and NKT-cell recruitment and viral clearance
    Ashley L St John
    Duke National University of Singapore, Singapore 169857
    Proc Natl Acad Sci U S A 108:9190-5. 2011
    ..These findings support expanding the defined role of immunosurveillance by MCs to include viral pathogens...
  81. pmc Type I IFN controls chikungunya virus via its action on nonhematopoietic cells
    Clementine Schilte
    Department of Immunology, Unité Immunobiologie des Cellules Dendritiques, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Exp Med 207:429-42. 2010
    ..This study defines an essential role for type I IFN, produced via cooperation between multiple host sensors and acting directly on nonhematopoietic cells, in the control of CHIKV...
  82. pmc An accessory to the 'Trinity': SR-As are essential pathogen sensors of extracellular dsRNA, mediating entry and leading to subsequent type I IFN responses
    Stephanie J DeWitte-Orr
    Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    PLoS Pathog 6:e1000829. 2010
    ..Furthermore, the implications of a cell surface receptor capable of recognizing extracellular RNA may exceed beyond viral immunity to mediating other important innate immune functions...
  83. pmc DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling
    Moeko Miyashita
    Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita ku, Sapporo 060 8638, Japan
    Mol Cell Biol 31:3802-19. 2011
    ..Expression of DDX60 promotes the binding of RIG-I to double-stranded RNA. Taken together, our analyses indicate that DDX60 is a novel antiviral helicase promoting RIG-I-like receptor-mediated signaling...
  84. pmc Innate immune recognition of an AT-rich stem-loop DNA motif in the Plasmodium falciparum genome
    Shruti Sharma
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Immunity 35:194-207. 2011
    ..Collectively, these observations implicate AT-rich DNA sensing via STING, TBK1 and IRF3-IRF7 in P. falciparum malaria...

Research Grants1

  1. Innate Intracellular Response to West Nile Virus
    Brenda Fredericksen; Fiscal Year: 2007
    ..A more complete understanding of the innate intracellular response to WNV will aid in the development of novel therapeutic approaches to combat WNV infections. [unreadable] [unreadable]..