Sp1

Summary

Gene Symbol: Sp1
Description: trans-acting transcription factor 1
Alias: 1110003E12Rik, AA450830, AI845540, Sp1-1, transcription factor Sp1
Species: mouse

Top Publications

  1. doi FoxO1 inhibits leptin regulation of pro-opiomelanocortin promoter activity by blocking STAT3 interaction with specificity protein 1
    Guoqing Yang
    Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667
    J Biol Chem 284:3719-27. 2009
  2. pmc TGFbeta1 regulation of vimentin gene expression during differentiation of the C2C12 skeletal myogenic cell line requires Smads, AP-1 and Sp1 family members
    Yongzhong Wu
    Department of Biochemistry and the Massey Cancer Center, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 0614, USA
    Biochim Biophys Acta 1773:427-39. 2007
  3. ncbi POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1
    Dong Kee Lee
    Department of Biochemistry and Molecular Biology, BK21 Project for Medical Sciences, Institute of Genetic Sciences, Yonsei University School of Medicine, 134 Shinchon dong, Seodaemoon ku, Seoul 120 752, Korea
    J Biol Chem 277:26761-8. 2002
  4. doi Hypoxia upregulates CD147 through a combined effect of HIF-1α and Sp1 to promote glycolysis and tumor progression in epithelial solid tumors
    Xia Ke
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Carcinogenesis 33:1598-607. 2012
  5. pmc STAT5 proteins are involved in down-regulation of iron regulatory protein 1 gene expression by nitric oxide
    Rafal Radoslaw Starzynski
    Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul Postepu 1, 05 552 Wolka Kosowska, Poland
    Biochem J 400:367-75. 2006
  6. doi Endothelin-1 induces glut1 transcription through enhanced interaction between Sp1 and NF-kappaB transcription factors
    Ying Shiun Kao
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan, Republic of China
    Cell Signal 20:771-8. 2008
  7. pmc Zinc-induced formation of a coactivator complex containing the zinc-sensing transcription factor MTF-1, p300/CBP, and Sp1
    Yong Li
    Department of Biochemistry and Molecular Biology, Mail Stop 3030, University of Kansas Medical Center, 39th and Rainbow Blvd, Kansas City, KS 66160 7421, USA
    Mol Cell Biol 28:4275-84. 2008
  8. doi O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors
    Kihong Lim
    School of Life Sciences and Biotechnology, Korea University, 5 1 Anam dong, Seongbuk gu, Seoul 136 701, Republic of Korea
    Biochem Biophys Res Commun 380:569-74. 2009
  9. pmc Bidirectional autoregulatory mechanism of metastasis-associated protein 1-alternative reading frame pathway in oncogenesis
    Da qiang Li
    Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, Washington, DC 20037, USA
    Proc Natl Acad Sci U S A 108:8791-6. 2011
  10. pmc Embryonic lethality and liver degeneration in mice lacking the metal-responsive transcriptional activator MTF-1
    C Gunes
    Institut fur Molekularbiologie, Abteilung II der Universität Zürich, Zurich, Switzerland
    EMBO J 17:2846-54. 1998

Research Grants

Scientific Experts

Detail Information

Publications138 found, 100 shown here

  1. doi FoxO1 inhibits leptin regulation of pro-opiomelanocortin promoter activity by blocking STAT3 interaction with specificity protein 1
    Guoqing Yang
    Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667
    J Biol Chem 284:3719-27. 2009
    ..that phospho-STAT3 activates POMC promoter in response to leptin signaling through a mechanism that requires an SP1-binding site in the POMC promoter...
  2. pmc TGFbeta1 regulation of vimentin gene expression during differentiation of the C2C12 skeletal myogenic cell line requires Smads, AP-1 and Sp1 family members
    Yongzhong Wu
    Department of Biochemistry and the Massey Cancer Center, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298 0614, USA
    Biochim Biophys Acta 1773:427-39. 2007
    ..DNA precipitation and ChIP assays suggest that c-Jun, c-Fos, Smad3 and Sp1/Sp3 interact over this region, but this interaction changes during myogenesis with TGFbeta1 induction.
  3. ncbi POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1
    Dong Kee Lee
    Department of Biochemistry and Molecular Biology, BK21 Project for Medical Sciences, Institute of Genetic Sciences, Yonsei University School of Medicine, 134 Shinchon dong, Seodaemoon ku, Seoul 120 752, Korea
    J Biol Chem 277:26761-8. 2002
    ..transcription factor, FBI-1, that recognizes the cis-element (bp -38 to -22) located just upstream of the core Sp1 binding sites (bp -22 to +22) of the ADH5/FDH minimal promoter (bp -38 to +61) in vitro and in vivo, as revealed by ..
  4. doi Hypoxia upregulates CD147 through a combined effect of HIF-1α and Sp1 to promote glycolysis and tumor progression in epithelial solid tumors
    Xia Ke
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
    Carcinogenesis 33:1598-607. 2012
    ..upregulation was mainly mediated by a combined effect of transcription factors HIF-1 and specificity protein 1 (Sp1) on the activation of CD147 promoter...
  5. pmc STAT5 proteins are involved in down-regulation of iron regulatory protein 1 gene expression by nitric oxide
    Rafal Radoslaw Starzynski
    Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul Postepu 1, 05 552 Wolka Kosowska, Poland
    Biochem J 400:367-75. 2006
    ..putative binding sites for transcription factor(s) regulated by NO and/or changes in intracellular iron level: Sp1 (promoter-selective transcription factor 1) and MTF1 (metal transcription factor 1), plus GAS (interferon-gamma-..
  6. doi Endothelin-1 induces glut1 transcription through enhanced interaction between Sp1 and NF-kappaB transcription factors
    Ying Shiun Kao
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan, Republic of China
    Cell Signal 20:771-8. 2008
    ..co-immunoprecipitation experiments, we were able to demonstrate that cooperative interaction between NF-kappaB and Sp1 were required to enhance Glut1 transcription in response to ET-1...
  7. pmc Zinc-induced formation of a coactivator complex containing the zinc-sensing transcription factor MTF-1, p300/CBP, and Sp1
    Yong Li
    Department of Biochemistry and Molecular Biology, Mail Stop 3030, University of Kansas Medical Center, 39th and Rainbow Blvd, Kansas City, KS 66160 7421, USA
    Mol Cell Biol 28:4275-84. 2008
    ..formation of a multiprotein complex containing MTF-1, the histone acetyltransferase p300/CBP, and the transcription factor Sp1. Down-regulation of endogenous p300 expression by small interfering RNA transfection significantly ..
  8. doi O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors
    Kihong Lim
    School of Life Sciences and Biotechnology, Korea University, 5 1 Anam dong, Seongbuk gu, Seoul 136 701, Republic of Korea
    Biochem Biophys Res Commun 380:569-74. 2009
    ..In this study, we show that O-GlcNAc inhibits a physical interaction between Sp1 and Elf-1 transcription factors, and negatively regulates transcription of placenta and embryonic expression ..
  9. pmc Bidirectional autoregulatory mechanism of metastasis-associated protein 1-alternative reading frame pathway in oncogenesis
    Da qiang Li
    Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, Washington, DC 20037, USA
    Proc Natl Acad Sci U S A 108:8791-6. 2011
    ..In this context, ARF interacts with transcription factor specificity protein 1 (SP1) and promotes its proteasomal degradation by enhancing its interaction with proteasome subunit regulatory particle ..
  10. pmc Embryonic lethality and liver degeneration in mice lacking the metal-responsive transcriptional activator MTF-1
    C Gunes
    Institut fur Molekularbiologie, Abteilung II der Universität Zürich, Zurich, Switzerland
    EMBO J 17:2846-54. 1998
    ..We also note that the MTF-1 null mutant phenotype bears some similarity to those of two other regulators of cellular stress response, namely c-Jun and NF-kappaB (p65/RelA)...
  11. pmc The transcription factors MTF-1 and USF1 cooperate to regulate mouse metallothionein-I expression in response to the essential metal zinc in visceral endoderm cells during early development
    G K Andrews
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160 7421, USA
    EMBO J 20:1114-22. 2001
    ..Expression of MT-I in visceral endoderm cells was dependent on maternal dietary zinc. Thus, the essential metal, zinc, apparently provides the signaling ligand that activates cell-specific MT-I expression in visceral endoderm cells...
  12. ncbi Suppression of HGF receptor gene expression by oxidative stress is mediated through the interplay between Sp1 and Egr-1
    Xianghong Zhang
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol Renal Physiol 284:F1216-25. 2003
    ..was localized at nucleotide position -223 to -68 of c-met promoter, in which reside an imperfect Egr-1 and three Sp1-binding sites...
  13. ncbi Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3
    Mingfeng Liu
    Multi Organ Transplant Program, Toronto General Hospital and the University of Toronto, Canada
    Eur J Biochem 270:2274-86. 2003
    ..cells revealed that the nucleoprotein complexes that form on this positive regulatory domain (PRD) contain Sp1/Sp3 family members, Oct-1, and Ets-1...
  14. ncbi Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of IL-10 in mouse macrophages
    Yi Wen Liu
    Graduate Institute of Biopharmaceutics, College of Life Science, National Chiayi University, Chiayi, Taiwan
    J Immunol 171:821-8. 2003
    ..have revealed that LPS can activate transcription of the IL-10 gene promoter through an SV40 promoter factor 1 (Sp1) binding site in mouse macrophage RAW264.7...
  15. ncbi Regulation of Egr-1, SRF, and Sp1 mRNA expression in contracting skeletal muscle cells
    Isabella Irrcher
    Dept of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    J Appl Physiol (1985) 97:2207-13. 2004
    ..The transcription factors Egr-1, Sp1, and serum response factor (SRF) are potentially important mediators of mitochondrial biogenesis based on the ..
  16. ncbi Cytokine-induced hepatic apoptosis is dependent on FGL2/fibroleukin: the role of Sp1/Sp3 and STAT1/PU.1 composite cis elements
    Mingfeng Liu
    Multi Organ Transplant Program, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada
    J Immunol 176:7028-38. 2006
    ..of fgl2 by IFN-gamma in macrophages involved a STAT1-dependent pathway, involving the composite cis elements Sp1/Sp3 and GAS/PU.1...
  17. ncbi K(v)1.5 potassium channel gene regulation by Sp1 transcription factor and oxidative stress
    Samuel J Fountain
    Institute of Membrane and Systems Biology, University of Leeds, Leeds, UK
    Am J Physiol Heart Circ Physiol 293:H2719-25. 2007
    ..The promoter contained CACCC nucleotide motifs, which we have shown to bind the Sp1 transcription factor in the aorta under physiological conditions in vivo...
  18. pmc Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1
    Karine Zaniolo
    Oncology and Molecular Endocrinology Research Center, Centre de Recherche du CHUL CHUQ and Département d Anatomie Physiologie, Universite Laval, Quebec, G1V 4G2, Canada
    BMC Mol Biol 8:96. 2007
    ..of the PARP-1 gene essentially rely on the opposite regulatory actions of two distinct transcription factors, Sp1 and NFI...
  19. doi Advanced glycation end products suppress neuropilin-1 expression in podocytes by a reduction in Sp1-dependent transcriptional activity
    Tzvetanka Bondeva
    Department of Internal Medicine III, Friedrich Schiller University, Jena, Germany
    Am J Nephrol 30:336-45. 2009
    ..The present study was undertaken to unravel the molecular mechanisms underlying AGE-BSA-mediated NRP1 suppression...
  20. ncbi Regulation of gene expression in the preimplantation mouse embryo: temporal and spatial patterns of expression of the transcription factor Sp1
    D M Worrad
    Department of Biology, University of Pennsylvania, Philadelphia 19104 6018, USA
    Mol Reprod Dev 46:268-77. 1997
    ..We and others have demonstrated that transcription factor Sp1 is present in the mouse oocyte and early cleavage stage preimplantation embryo...
  21. ncbi Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation
    M Marin
    Erasmus University Rotterdam, Department of Cell Biology, The Netherlands
    Cell 89:619-28. 1997
    b>Transcription factor Sp1 has been implicated in the expression of many genes...
  22. pmc Histone deacetylase 1 can repress transcription by binding to Sp1
    A Doetzlhofer
    Institute of Molecular Biology, Vienna Biocenter, University of Vienna, Vienna, Austria
    Mol Cell Biol 19:5504-11. 1999
    The members of the Sp1 transcription factor family can act as both negative and positive regulators of gene expression. Here we show that Sp1 can be a target for histone deacetylase 1 (HDAC1)-mediated transcriptional repression...
  23. ncbi The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1
    Eric Lecuyer
    Clinical Research Institute of Montreal and from the Departments of Pharmacology, Biochemistry, and Molecular Biology, Universite de Montreal, Quebec, Canada
    Blood 100:2430-40. 2002
    ..ubiquitous (E2A, LIM- domain binding protein 1 [Ldb-1]) factors, is tethered to DNA via a specificity protein 1 (Sp1) motif, through direct interactions between elements of the SCL complex and the Sp1 zinc finger protein...
  24. ncbi The repressor element silencing transcription factor (REST)-mediated transcriptional repression requires the inhibition of Sp1
    Valérie Plaisance
    Department of Internal Medicine, University of Lausanne, 1005 Lausanne, Switzerland
    J Biol Chem 280:401-7. 2005
    ..Herein, we showed that the transcriptional factor Sp1 was required for expression of most REST target genes both in insulin-secreting cells and neuronal-like cells where ..
  25. pmc The family feud: turning off Sp1 by Sp1-like KLF proteins
    Gwen Lomberk
    Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55901, USA
    Biochem J 392:1-11. 2005
    b>Sp1 is one of the best characterized transcriptional activators. The biological importance of Sp1 is underscored by the fact that several hundreds of genes are thought to be regulated by this protein...
  26. ncbi Sp1 is up-regulated in cellular and transgenic models of Huntington disease, and its reduction is neuroprotective
    Zhihua Qiu
    Massachusetts General Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA
    J Biol Chem 281:16672-80. 2006
    ..Our data suggest that enhancement of transcription factor Sp1 contributes to the pathology of HD and demonstrates that its suppression is beneficial.
  27. pmc Modulation of Bone Morphogenetic Protein (BMP) 2 gene expression by Sp1 transcription factors
    Junwang Xu
    Biochemistry and Molecular Biology, UMDNJ New Jersey Medical School, Newark, NJ 07101 1709, USA
    Gene 392:221-9. 2007
    ..BMP2 gene polymorphisms have been linked to osteoporosis and osteoarthritis. Sp1 and related proteins are widely expressed regulators of gene expression whose transcription activating abilities ..
  28. ncbi Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects
    Imme Krüger
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Marburg, Germany
    Dev Dyn 236:2235-44. 2007
    The ubiquitously expressed zinc finger transcription factors Sp1 and Sp3 play critical roles in embryonic development. Sp1 knockout mice die around embryonic day 10.5. Mice lacking Sp3 are postnatal lethal...
  29. ncbi v-Src-mediated down-regulation of SSeCKS metastasis suppressor gene promoter by the recruitment of HDAC1 into a USF1-Sp1-Sp3 complex
    Yahao Bu
    Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    J Biol Chem 282:26725-39. 2007
    ..alpha proximal promoter as the minimal v-Src-responsive element, which contains E- and GC-boxes bound by USF1 and Sp1/Sp3, respectively. Both E- and GC-boxes are crucial for v-Src-responsive and basal promoter activities...
  30. ncbi OX40 gene expression is up-regulated by chromatin remodeling in its promoter region containing Sp1/Sp3, YY1, and NF-kappa B binding sites
    Yukiko Tone
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Immunol 179:1760-7. 2007
    ..We have shown in this study that basal OX40 promoter activity is regulated by constitutively expressed Sp1/Sp3 and YY1 transcription factors...
  31. pmc Differential regulation of GPR54 transcription by specificity protein-1 and partial estrogen response element in mouse pituitary cells
    Mia C DeFino
    Department of Pharmacology, Box 357280, University of Washington, Seattle, WA 98195 7280, USA
    Biochem Biophys Res Commun 393:603-8. 2010
    ..Supporting experiments show transcription factor SP1 binds directly to the mGPR54 promoter region and activates gene expression...
  32. pmc Regulation of embryonic kidney branching morphogenesis and glomerular development by KISS1 receptor (Gpr54) through NFAT2- and Sp1-mediated Bmp7 expression
    Tingfang Yi
    Department of Molecular and Cellular Medicine, Texas A and M University Health Science Center, Institute of BioScience and Technology, Center for Cancer and Stem Cell Biology, Houston, Texas 77030, USA
    J Biol Chem 285:17811-20. 2010
    ..Using chromatin immunoprecipitation and luciferase assays, we demonstrate that Gpr54 regulates NFAT2- and Sp1-mediated Bmp7 transcription...
  33. pmc Overexpression of antioxidant enzymes upregulates aryl hydrocarbon receptor expression via increased Sp1 DNA-binding activity
    Tian Tang
    Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA
    Free Radic Biol Med 49:487-92. 2010
    ..The objective of this study was to investigate the regulatory role of specificity protein-1 (Sp1) in AhR expression in MAECs that overexpress Cu/Zn-SOD and/or catalase...
  34. pmc Interactive roles of Ets-1, Sp1, and acetylated histones in the retinoic acid-dependent activation of guanylyl cyclase/atrial natriuretic peptide receptor-A gene transcription
    Prerna Kumar
    Department of Physiology, Tulane University Health Sciences Center and School of Medicine, New Orleans, Louisiana 70112, USA
    J Biol Chem 285:37521-30. 2010
    ..The present study was conducted to examine the interactive roles of all-trans retinoic acid (ATRA), Ets-1, Sp1, and histone acetylation on the transcriptional regulation and function of the Npr1 gene...
  35. pmc Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter
    Samuel Rivero
    Facultad de Medicina Centro Regional de Investigaciones Biomédicas, Universidad de Castilla La Mancha, Calle Almansa 14, 02006 Albacete, Spain
    BMC Mol Biol 12:52. 2011
    ..The expression of the gene Dlk2 is coordinated with that of Dlk1 in several tissues and cell lines. Unlike Dlk1, the mouse Dlk2 gene and its locus at chromosome 17 are not fully characterized...
  36. ncbi mSharp-1/DEC2, a basic helix-loop-helix protein functions as a transcriptional repressor of E box activity and Stra13 expression
    Sameena Azmi
    Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:20098-109. 2003
    ..Moreover, mSharp-1 directly interacts with the transcriptional activator Sp1 and impairs Sp1 induction of Stra13 promoter...
  37. ncbi A role for E2F6 in the restriction of male-germ-cell-specific gene expression
    Michael Pohlers
    Children s Hospital, Laboratory for Molecular Biology, Charité CCM, Humboldt University, Ziegelstrasse 5 9, D 10098 Berlin, Germany
    Curr Biol 15:1051-7. 2005
    ..Thus, E2F6 is essential for the long-term somatic silencing of certain male-germ-cell-specific genes, but it is dispensable for cell-cycle regulation...
  38. pmc Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts
    Wenli Ma
    Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA
    Biol Reprod 79:289-300. 2008
    ..in regulation of gene expression, it is important to understand how levels of Krüpple-like transcription factors SP1 and SP3 change in germ cells during spermatogenesis...
  39. ncbi Structural and functional characterization of the mouse multidrug resistance gene family
    R Dhir
    Department of Biochemistry, McGill University, Montreal, Canada
    Bull Cancer 77:1125-9. 1990
    ..Finally, although mdr1 and mdr3 can confer multidrug resistance, drug survival characteristics of mdr1 and mdr3 transfectants indicate that both proteins have overlapping but distinct substrate specificities...
  40. pmc An alternatively spliced form of the transcription factor Sp1 containing only a single glutamine-rich transactivation domain
    S P Persengiev
    Worcester Foundation for Biomedical Research, Shrewsbury, MA 01545, USA
    Proc Natl Acad Sci U S A 92:9107-11. 1995
    ..The promoter-specific transcription factor Sp1 contains two glutamine-rich transcriptional activation domains (A and B) that mediate direct interactions ..
  41. ncbi Communication between NF-kappa B and Sp1 controls histone acetylation within the proximal promoter of the monocyte chemoattractant protein 1 gene
    Gunther H Boekhoudt
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Immunol 170:4139-47. 2003
    ..chemoattractant protein 1 gene (MCP-1) by TNF occurs through an NF-kappaB-dependent distal regulatory region and an Sp1-dependent proximal regulatory region that are separated by 2.2 kb of sequence...
  42. ncbi Differential utilization of the promoter of peripheral-type benzodiazepine receptor by steroidogenic versus nonsteroidogenic cell lines and the role of Sp1 and Sp3 in the regulation of basal activity
    Christoforos Giatzakis
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Endocrinology 145:1113-23. 2004
    ..the absence of TATA or CCAAT boxes, but the presence of many putative transcription factor-binding sites, including Sp1/Sp3, AP2, Ik2, AP1, SOX, GATA, and SRY...
  43. ncbi Antifibrotic effect via the regulation of transcription factor Sp1 in lung fibrosis
    Yoon Seup Kum
    Department of Pathology, Catholic University of Daegu, College of Medicine, 3056 6, Daemyung 4 dong, Nam Gu, Daegu 705 718, Republic of Korea
    Biochem Biophys Res Commun 363:368-74. 2007
    The aim of this study is to evaluate the antifibrotic effect of ring-type Sp1 decoy oligonucleotides (ODNs) through blocking the transcription of transforming growth factor (TGF)-beta1 and its downstream target genes...
  44. pmc Contributions of specificity protein-1 and steroidogenic factor 1 to Adcy4 expression in Y1 mouse adrenal cells
    Xianliang Rui
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada
    Endocrinology 149:3668-78. 2008
    ..As determined by site-specific mutagenesis, the 5'-most Sp1/Sp3-site enhances promoter activity, whereas the middle Sp1/Sp3 and SF1 sites each repress Adcy4 promoter activity...
  45. pmc Central role of Sp1-regulated CD39 in hypoxia/ischemia protection
    Holger K Eltzschig
    Department of Anesthesiology and Perioperative Medicine, University of Colorado Health Sciences Center, Denver, CO, USA
    Blood 113:224-32. 2009
    ..chromatin immunoprecipitation, and inhibition by antisense, revealed a critical role for transcription-factor Sp1 in hypoxia-induction of CD39...
  46. doi Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytes
    Sebastian Zellmer
    Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
    Hepatology 52:2127-36. 2010
    ..factor binding sites (TFBS) for ETF (TEA domain family member 2), E2F1 (E2F transcription factor 1), and SP-1 (Sp1 transcription factor) (P < 0.001), all depending on MAPK signaling...
  47. pmc A novel function of apolipoprotein E: upregulation of ATP-binding cassette transporter A1 expression
    Yanfeng Zhao
    Department of Physiology, Wuhan University School of Basic Medical Science, Wuhan, People s Republic of China
    PLoS ONE 6:e21453. 2011
    ..In addition, these lipoproteins increased the level of phosphorylated specificity protein 1 (Sp1) and the amount of Sp1 bound to the ABCA1 promoter...
  48. pmc P14ARF inhibits human glioblastoma-induced angiogenesis by upregulating the expression of TIMP3
    Abdessamad Zerrouqi
    Laboratory of Molecular Neuro Oncology, Department of Neurosurgery, School of Medicine, Emory University, Atlanta, Georgia 30322, USA
    J Clin Invest 122:1283-95. 2012
    ..Mechanistically, this regulation occurred at the gene transcription level and was controlled by HDM2-SP1 interplay, where P14ARF relieved a dominant negative interaction of HDM2 with SP1...
  49. pmc FoxO1 inhibits sterol regulatory element-binding protein-1c (SREBP-1c) gene expression via transcription factors Sp1 and SREBP-1c
    Xiong Deng
    Department of Medicine, Memphis Veterans Affairs Medical Center, Memphis, Tennessee 38104, USA
    J Biol Chem 287:20132-43. 2012
    ..Insulin stimulates SREBP-1c transcription through Sp1 and via "feed forward" regulation by newly synthesized SREBP-1c...
  50. doi Osterix induces Col1a1 gene expression through binding to Sp1 sites in the bone enhancer and proximal promoter regions
    María José Ortuño
    Departament de Ciències Fisiològiques II, Universitat de Barcelona, IDIBELL, L Hospitalet de Llobregat, Spain
    Bone 52:548-56. 2013
    ..We identified consensus Sp1 sequences, located in the proximal promoter and in the bone-enhancer, as Osterix binding regions in the Col1a1 ..
  51. pmc Nuclear factor κB2 p52 protein has a role in antiviral immunity through IκB kinase epsilon-dependent induction of Sp1 protein and interleukin 15
    Sarah L Doyle
    Immunology Research Centre, Trinity Biomedical Sciences Institute, School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
    J Biol Chem 288:25066-75. 2013
    ..We identify a novel NFκB2 binding site in the promoter of the transcription factor Sp1 that is required for Sp1 gene transcription activated by poly(I:C)...
  52. ncbi Transcription factor Sp1 is expressed by three different developmentally regulated messenger ribonucleic acids in mouse spermatogenic cells
    S P Persengiev
    Neurobiology Group, Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA
    Endocrinology 137:638-46. 1996
    ..Recent evidence suggests that the DNA-binding protein Sp1 functions as an important trans-activator during cell development and differentiation...
  53. ncbi A critical role of Sp1- and Ets-related transcription factors in maintaining CTL-specific expression of the mouse perforin gene
    B S Youn
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Immunol 157:3499-509. 1996
    ..The proximal region in PFP9a20 contained two potential Sp1 binding sites (GC box and GT box) and one Ets binding site (EBS)...
  54. pmc Targeted disruption of mouse Yin Yang 1 transcription factor results in peri-implantation lethality
    M E Donohoe
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 19:7237-44. 1999
    ..Our studies demonstrate an essential function for YY1 in the development of the mouse embryo...
  55. pmc Histone deacetylase inhibition is associated with transcriptional repression of the Hmga2 gene
    Mark Ferguson
    Laboratory of Biochemistry and Molecular Biology, The Wheeler Institute for Biomedical Research, Johns Hopkins University Bayview Campus, Baltimore, MD 21224, USA
    Nucleic Acids Res 31:3123-33. 2003
    ..chromatin immunoprecipitation (X-ChIP) analysis revealed a 5-6-fold decrease in endogenous Hmga2 promoter bound Sp1 and Sp3 proteins following TSA treatment in parallel with observed loss of acetylated histone H3 and H4...
  56. ncbi c-Jun and Sp1 family are critical for retinoic acid induction of the lamin A/C retinoic acid-responsive element
    Koichi Okumura
    Biomolecular Engineering Research Institute, 6 2 3 Furuedai, Suita, Osaka 565 0874, Japan
    Biochem Biophys Res Commun 320:487-92. 2004
    ..P19 cells as a model system, that the lamin A/C promoter has a retinoic acid-responsive element (L-RARE), and that Sp1 and Sp3 bind the CACCC box of the L-RARE...
  57. ncbi Sp1 and Sp3 transcription factors mediate trichostatin A-induced and basal expression of extracellular superoxide dismutase
    Igor N Zelko
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Free Radic Biol Med 37:1256-71. 2004
    ..gene regulation, we analyzed EC-SOD's proximal promoter region, which revealed two previously unknown but putative Sp1 cis elements...
  58. ncbi Interleukin-6 induces transcriptional activation of vascular endothelial growth factor (VEGF) in astrocytes in vivo and regulates VEGF promoter activity in glioblastoma cells via direct interaction between STAT3 and Sp1
    Sébastien Loeffler
    Division of Neuropathology, Institute of Pathology, University of Bern, Bern, Switzerland
    Int J Cancer 115:202-13. 2005
    ..Electrophoretic mobility shift and supershift assays revealed binding of Sp1 and Sp3 to the -88/-50 element upon IL-6 stimulation...
  59. ncbi Augmented IL-10 production and redox-dependent signaling pathways in glucose-6-phosphate dehydrogenase-deficient mouse peritoneal macrophages
    Jeanette Wilmanski
    Graduate School of Biomedical Sciences, UMDNJ New Jersey Medical School, Newark, 07103, USA
    J Leukoc Biol 78:85-94. 2005
    ..Alterations in signaling pathways and associated changes in cytokine production may play a role in modulating the inflammatory responses following bacterial or malarial infections in G6PD deficiency...
  60. pmc Constitutive expression of murine decay-accelerating factor 1 is controlled by the transcription factor Sp1
    David M Cauvi
    Department of Molecular and Experimental Medicine, W M Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 177:3837-47. 2006
    ..7 cells. Mutational analyses of the promoter region combined with Sp1-specific ELISA showed that the transcription factor Sp1 is required for basal transcription and LPS-induced expression of the Daf1 gene...
  61. doi Up-regulated expression of Sp1 protein coincident with a viral protein in human and mouse differentiating keratinocytes may act as a cell differentiation marker
    Bo Li
    Diamantina Institute for Cancer Immunology and Metabolic Medicine, The University of Queensland, Research Extension, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia
    Differentiation 76:1068-80. 2008
    b>Sp1 is a transcription factor that regulates expression of mammalian and viral genes and is involved in different facets of cellular functions in eukaryotic cells...
  62. doi ATM-mediated transcriptional elevation of prion in response to copper-induced oxidative stress
    Kefeng Qin
    Departments of Pathology and Microbiology Immunology and Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 284:4582-93. 2009
    ..the mitogen-activated protein kinase kinase/extracellular-related kinases/extracellular-related kinases (MEK/ERK)/Sp1 pathway. As results, Sp1 and p53 bind to the PrP promoter, leading to increase PrP(C) expression...
  63. doi Tissue inhibitor of metalloproteinase-3 inhibits neonatal mouse cardiomyocyte proliferation via EGFR/JNK/SP-1 signaling
    Lamis Hammoud
    Department of Physiology, Univ of Western Ontario, London, Ontario, Canada N6A 5C1
    Am J Physiol Cell Physiol 296:C735-45. 2009
    ..We conclude that TIMP-3 inhibits neonatal mouse cardiomyocyte proliferation by upregulating p27 expression. The effects of TIMP-3 are mediated via inhibition of EGFR expression/phosphorylation, and decreases in JNK and SP-1 signaling...
  64. doi Regulation of the mouse CTP: phosphoethanolamine cytidylyltransferase gene Pcyt2 during myogenesis
    Lin Zhu
    Department of Human Health and Nutritional Sciences, Animal Science and Nutrition Building, Room 346, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    Gene 447:51-9. 2009
    ..Transcription factors Sp1 and Sp3 bind to regions A (-508/-378 bp) and C (-157/-111 bp), and muscle-specific differentiation factor MyoD ..
  65. doi Sp1 mediate hypoxia induced ephrinB2 expression via a hypoxia-inducible factor independent mechanism
    Marcus Sohl
    Department of Cell and Molecular Biology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Biochem Biophys Res Commun 391:24-7. 2010
    ..MAZ and Sp1 are known to regulate transcription together and have been shown to bind to the same sites within promoters...
  66. pmc N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells
    Guergana Tasseva
    Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 286:1061-73. 2011
    ..Transcription factor-binding sites, such as a GC-box cluster that binds Sp1/Sp3/Sp4 and N-Myc, and a degenerate E-box motif that interacts with Tal1 and E47, were identified...
  67. doi Sp1, acetylated histone-3 and p300 regulate TRAIL transcription: mechanisms of PDGF-BB-mediated VSMC proliferation and migration
    Nor Saadah M Azahri
    Centre for Vascular Research, University of New South Wales, Sydney, NSW 2052, Australia
    J Cell Biochem 113:2597-606. 2012
    ..was increased by PDGF-BB and subsequently inhibited by dominant-negative Sp1, suggesting that the transcription factor Sp1 plays a role...
  68. ncbi CTCF and Sp1 interact with the Murine gammaherpesvirus 68 internal repeat elements
    Hannah C Stevens
    Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine, University of Liverpool, Liverpool, L69 3GE, UK
    Virus Genes 45:265-73. 2012
    ..This cell line model was used to investigate transcription factor occupancy (CCCTC binding factor [CTCF] and Sp1) of the two internal repeat elements in the viral genome during latency and reactivation using chromatin ..
  69. pmc The specificity protein factor Sp1 mediates transcriptional regulation of P2X7 receptors in the nervous system
    Paula García-Huerta
    Departamento de Bioquimica y Biologia Molecular, Universidad Complutense de Madrid, 28040 Madrid, Spain
    J Biol Chem 287:44628-44. 2012
    ..Here we reported the first molecular and functional evidence that Specificity protein 1 (Sp1) transcription factor plays a pivotal role in the transcriptional regulation of P2X7 receptor...
  70. pmc Macrophage migration arrest due to a winning balance of Rac2/Sp1 repression over β-catenin-induced PLD expression
    Francis J Speranza
    1 Dept of Biochemistry and Molecular Biology, 3640 Colonel Glenn Highway, Dayton, OH 45435, USA
    J Leukoc Biol 94:953-62. 2013
    ..links the Rac2-dependent arrest of chemotaxis to decreased expression of PLD2 through the transcription regulator Sp1. Prolonged Rac2 activity leads to nuclear overactivation of Sp1, which acts as a repressor for PLD2...
  71. pmc A crucial role for the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification
    Jane Gilmour
    School of Cancer Sciences, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
    Development 141:2391-401. 2014
    ..is regulated by the interplay of tissue-specific and ubiquitously expressed transcription factors, such as Sp1. Sp1 knockout mice die in utero with multiple phenotypic aberrations, but the underlying molecular mechanism of ..
  72. doi Ischemia/reperfusion-induced upregulation of TIGAR in brain is mediated by SP1 and modulated by ROS and hormones involved in glucose metabolism
    Meiling Sun
    Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro Psycho Diseases, Soochow University School of Pharmaceutical Science, Suzhou 215123, China
    Neurochem Int 80:99-109. 2015
    ..The transcription factor SP1 was induced by ischemia/reperfusion ahead of TIGAR upregulation...
  73. doi Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function
    Marjolein Meinders
    Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    Blood 125:1957-67. 2015
    b>Sp1 and Sp3 belong to the specificity proteins (Sp)/Krüppel-like transcription factor family. They are closely related, ubiquitously expressed, and recognize G-rich DNA motifs...
  74. ncbi Transcription suppression of thromboxane receptor gene by peroxisome proliferator-activated receptor-gamma via an interaction with Sp1 in vascular smooth muscle cells
    Akira Sugawara
    Division of Nephrology, Department of Medicine, Tohoku University Graduate School of Medicine, 1 1 Seiryo cho, Aoba ku, Sendai 980 8574, Japan
    J Biol Chem 277:9676-83. 2002
    ..Electrophoretic mobility shift assays also showed that the sequence was bound by Sp1 but not by PPAR-gamma, and the formation of a Sp1 small middle dotDNA complex was inhibited either by coincubation ..
  75. pmc Histone deacetylase 3 mediates allergic skin inflammation by regulating expression of MCP1 protein
    Youngmi Kim
    Department of Biochemistry, Kangwon National University, Chunchon 200 701, Korea
    J Biol Chem 287:25844-59. 2012
    ..FcεRI was necessary for induction of HDAC3 and MCP1. ChIP assays showed that HDAC3, in association with Sp1 and c-Jun, was responsible for induction of MCP1 expression. TSA exerted a negative effect on induction of MCP1...
  76. pmc Transcriptional regulation of N-acetylglutamate synthase
    Sandra Kirsch Heibel
    Center for Genetic Medicine Research, Children s National Medical Center, Washington, DC, United States of America
    PLoS ONE 7:e29527. 2012
    ..DNA-protein pull-down assays and chromatin immunoprecipitation confirmed binding of Sp1 and CREB, but not C/EBP in the promoter and HNF-1 and NF-Y, but not SMAD3 or AP-2 in the enhancer...
  77. ncbi Difference in the genomic organizations of the related transcription factors Sp1 and Krox-20; possible evolutionary significance
    A Chestier
    Laboratoire de Genetique Moleculaire, CNRS URA 1302, Ecole Normale Superieure, Paris, France
    DNA Seq 2:325-7. 1992
    We report here part of the genomic sequence of the mouse homolog of the human gene encoding the transcription factor Sp1. This gene belongs to a subfamily of zinc finger proteins, which includes another transcription factor, Krox-20...
  78. ncbi Zinc finger protein genes in the mouse genome
    A Ashworth
    Chester Beatty Laboratories, Institute of Cancer Research, London, UK
    Mamm Genome 1:196-200. 1991
  79. ncbi Localization of the gene for the trans-acting transcription factor Sp1 to the distal end of mouse chromosome 15
    J D Saffer
    Jackson Laboratory, Bar Harbor, Maine 04609
    Genomics 8:571-4. 1990
    The mouse chromosomal location for the gene (Sp1-1) encoding the trans-acting transcription factor Sp1 has been determined...
  80. ncbi A novel, conserved gene of the rat that is developmentally regulated in the testis
    L Walter
    Division of Immunogenetics, University of Gottingen, Germany
    Mamm Genome 5:216-21. 1994
    ..Analysis of rat RNA from different stages of spermatogenesis indicated that accumulation of the short transcript occurred mainly postmeiotically. The rat Tegt gene maps to Chromosome (Chr) 7, and its mouse homolog to Chr 15...
  81. ncbi Identification of a novel conserved human gene, TEGT
    L Walter
    Division of Immunogenetics, University of Gottingen, Germany
    Genomics 28:301-4. 1995
    ..The presence of a nuclear targeting motif indicates that the gene product might localize to the nucleus. The TEGT gene maps to human chromosome 12q12-q13 and belongs to a syntenic group, which is conserved in human, mouse, and rat...
  82. ncbi Transcriptional regulation of murine beta1,4-galactosyltransferase in somatic cells. Analysis of a gene that serves both a housekeeping and a mammary gland-specific function
    B Rajput
    Cell Structure and Function Laboratory, Oncology Center Department of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21287 8937, USA
    J Biol Chem 271:5131-42. 1996
    ..1-kb start site is occupied mainly by the ubiquitous factor Sp1. In contrast, the region adjacent to the 3...
  83. pmc A role for Sp and nuclear receptor transcription factors in a cardiac hypertrophic growth program
    M N Sack
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 94:6438-43. 1997
    ..Antibody "supershift" studies demonstrated that members of the Sp (Sp1, Sp3) and nuclear hormone receptor [chicken ovalbumin upstream promoter transcription factor (COUP-TF)/erbA-related ..
  84. ncbi Sp family transcription factors regulate expression of rat D2 dopamine receptor gene
    S Yajima
    Genetic Pharmacology Unit, Experimental Therapeutics Branch, National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892, USA
    DNA Cell Biol 17:471-9. 1998
    ..receptor gene is transcribed from a TATA-less promoter that has an initiator-like sequence and several putative Sp1 binding sites...
  85. ncbi Combinatorial cis-acting elements control tissue-specific activation of the cardiac troponin I gene in vitro and in vivo
    R Di Lisi
    Department of Biomedical Sciences, University of Padova, 35121 Padova, Italy
    J Biol Chem 273:25371-80. 1998
    ..Functional analysis indicates that MEF2/Oct-1, Sp1, and GATA regulatory elements are required for optimal gene activation because selective mutations produce weak or ..
  86. ncbi Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing
    T Takahara
    Department of Life Sciences Chemistry, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro, Tokyo 153 8902, Japan
    J Biol Chem 275:38067-72. 2000
    b>Sp1 is one of the well documented transcription factors, but the whole structure of human Sp1 has not been determined yet...
  87. ncbi Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene
    H Gollner
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    Genes Cells 6:689-97. 2001
    Sp4 is a zinc finger transcription factor which is closely related to Sp1 and Sp3. All three proteins recognize the same DNA elements and can act as transcriptional activators through glutamine-rich activation domains...
  88. ncbi Sp1 is the major fasl gene activator in abnormal CD4(-)CD8(-)B220(+) T cells of lpr and gld mice
    S Xiao
    Department of Medicine, Boston University School of Medicine, Boston, USA
    Eur J Immunol 31:3339-48. 2001
    ..Specific binding sites for Sp1, NFAT, Egr, and NF-kappaB have been identified in the promoter region of the fasl gene...
  89. ncbi Lipopolysaccharide down-regulates Sp1 binding activity by promoting Sp1 protein dephosphorylation and degradation
    Xiaobing Ye
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Long Island Jewish Medical Center, Long Island Campus for the Albert Einstein College of Medicine, New Hyde Park, New York 11040, USA
    J Biol Chem 277:31863-70. 2002
    We examined the in vivo effect of lipopolysaccharide (LPS) on Sp1 (promoter-selective transcription factor 1) DNA binding activity and studied the mechanisms involved in mouse lungs...
  90. ncbi Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury
    Luisa M Botella
    Centro de Investigaciones Biologicas, Consejo Superior Investigaciones Cientificas CSIC, Madrid, Spain
    Blood 100:4001-10. 2002
    ..a GC-rich tract required for basal activity of the endoglin promoter driven by the related GC box binding protein, Sp1. In cells lacking Sp1 and KLF6, transfected KLF6 and Sp1 cooperatively transactivated the endoglin promoter and ..
  91. ncbi Activation of CTP:phosphocholine cytidylyltransferase alpha expression during the S phase of the cell cycle is mediated by the transcription factor Sp1
    Claudia Banchio
    Department of Biochemistry and Canadian Institutes of Health Research Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
    J Biol Chem 278:32457-64. 2003
    ..We now demonstrate that increased binding of the transcription factor Sp1 to the proximal promoter of CT alpha is responsible for increased transcription during the S phase...
  92. ncbi Regulation of aldehyde reductase expression by STAF and CHOP
    Oleg A Barski
    Harry B and Aileen Gordon Diabetes Research Laboratory, Molecular Diabetes and Metabolism Section, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Genomics 83:119-29. 2004
    ..The 3' element binds transcription factors of the Sp1 family...
  93. pmc Structural basis of the interaction between the AAA ATPase p97/VCP and its adaptor protein p47
    Ingrid Dreveny
    Centre for Structural Biology, Department of Biological Sciences, Imperial College London, South Kensington Campus, London, UK
    EMBO J 23:1030-9. 2004
    ..We also propose a classification for ubiquitin-like domains containing or lacking a longer S3/S4 loop...
  94. ncbi Superoxide dismutase-3 promotes full expression of the EPO response to hypoxia
    Hagir B Suliman
    Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA
    Blood 104:43-50. 2004
    ....
  95. ncbi Phosphorylation of Sp1 by cyclin-dependent kinase 2 modulates the role of Sp1 in CTP:phosphocholine cytidylyltransferase alpha regulation during the S phase of the cell cycle
    Claudia Banchio
    Department of Biochemistry, Canadian Institutes of Health Research Group in Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    J Biol Chem 279:40220-6. 2004
    ..E. (2001) J. Biol. Chem. 276, 43688-43692) and that this activation is driven by increased binding of Sp1 to the CTalpha promoter (Banchio, C., Schang, L. M., and Vance, D. E. (2003) J. Biol. Chem. 278, 32457-32464)...
  96. pmc Induction of vascular smooth muscle alpha-actin gene transcription in transforming growth factor beta1-activated myofibroblasts mediated by dynamic interplay between the Pur repressor proteins and Sp1/Smad coactivators
    Sukanya V Subramanian
    Department of Physiology and Cell Biology, Dorothy M Davis Heart and Lung Research Institute, College of Medicine and Public Health, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 15:4532-43. 2004
    ..The SMA enhancer contains tandem sites for the Sp1 transcriptional activator protein and Puralpha and beta repressor proteins...
  97. ncbi Distinct transcriptional control and action of fibroblast growth factor receptor 4 in differentiating skeletal muscle cells
    ShunJiang Yu
    Department of Medicine, Mount Sinai Hospital and University of Toronto, The Freeman Centre for Endocrine Oncology and The Ontario Cancer Institute, Toronto, Ontario, Canada
    Lab Invest 84:1571-80. 2004
    ..These complexes were efficiently competed by an Sp-type oligonucleotide and were supershifted by Sp1 and by Sp3 antibodies...
  98. ncbi Identification of an Sp factor-dependent promoter in GCET, a gene expressed at high levels in germinal center B cells
    John W Steinke
    Allergy Division, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
    Mol Immunol 41:1145-53. 2004
    ..Together, these data provide the first analysis of regulatory modules necessary for GCET expression, a model for GC B cell-specific transcription...
  99. ncbi Sp1 site is crucial for the mouse claudin-19 gene expression in the kidney cells
    John M Luk
    Department of Surgery, Hong Kong Jockey Club Clinical Research Centre, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
    FEBS Lett 578:251-6. 2004
    ..resided in the -39 to -108 region, which contained a number of binding sites for transcription factors including Sp1. Site-directed mutagenesis using specific oligo probes confirmed that Sp1 was crucial for Cldn19 transactivation in ..
  100. ncbi Selenium attenuates expression of MnSOD and uncoupling protein 2 in J774.2 macrophages: molecular mechanism for its cell-death and antiinflammatory activity
    Shani Shilo
    The School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovet, Israel
    Antioxid Redox Signal 7:276-86. 2005
    ..This cellular effect was due to inhibition of specificity protein-1 (Sp1) binding to its DNA binding site...

Research Grants3

  1. METALLOTHIONEIN IN REPRODUCTION AND DEVELOPMENT
    Glen Andrews; Fiscal Year: 2002
    ..A testable hypothesis is that misexpression of MT will alter metal homeostasis during pregnancy causing increased sensitivity to dietary Zn deficiency and reduced sensitivity to Cd toxicity. ..
  2. Environmental Toxicology Using Transgenic Models
    Glen Andrews; Fiscal Year: 2005
    ..Functions of MTF-l during development will be examined in Zebrafish using morpholino antisense oligonucleotides, whole mount in situ hybridization and morphometric analyses of developing embryos. ..
  3. Molecular Biology of Mammalian Zinc Homeostasis
    Glen Andrews; Fiscal Year: 2006
    ..The co-I., Dr. Eide is an expert and leader in the field of ZIP transport function, and our collaborator, Dr. Palmiter is an expert in gene targeting in mice. ..