INS

Summary

Gene Symbol: INS
Description: insulin
Alias: insulin, preproinsulin, proinsulin
Species: pig

Top Publications

  1. pmc The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network
    Martial Boutchueng-Djidjou
    From the Département de Pédiatrie, Laboratoire de Biologie Cellulaire
    Mol Cell Proteomics 14:1079-92. 2015
  2. doi Amino acid sequence determinants in self-assembly of insulin chiral amyloid superstructures: role of C-terminus of B-chain in association of fibrils
    Viktoria Babenko
    Department of Chemistry, University of Warsaw, Pasteura 1, 02 093 Warsaw, Poland
    FEBS Lett 587:625-30. 2013
  3. doi Thermodynamic contributions to the stability of the insulin hexamer
    George P Lisi
    Department of Chemistry, Dartmouth College, 6128 Burke Laboratory, Hanover, New Hampshire 03755, United States
    Biochemistry 53:3576-84. 2014
  4. doi The study of regulatory effects of Pdx-1, MafA and NeuroD1 on the activity of porcine insulin promoter and the expression of human islet amyloid polypeptide
    Xiao Dan Liu
    Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, People s Republic of China
    Mol Cell Biochem 394:59-66. 2014
  5. ncbi Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved
    Giuseppe Pandini
    Istituto di Medicina Interna, Malattie Endocrine e del Metabolismo, University of Catania, Ospedale Garibaldi, 95123 Catania, Italy
    J Biol Chem 277:39684-95. 2002
  6. ncbi Cultured trout liver cells: utilization of substrates and response to hormones
    H Segner
    Department of Zoology II, University of Karlsruhe, FRG
    In Vitro Cell Dev Biol Anim 30:306-11. 1994
  7. ncbi Growth hormone, interferon-gamma, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1
    L S Argetsinger
    Department of Physiology, University of Michigan Medical School, Ann Arbor 48109 0622, USA
    J Biol Chem 270:14685-92. 1995
  8. ncbi Evidence for a functional role of Shc proteins in mitogenic signaling induced by insulin, insulin-like growth factor-1, and epidermal growth factor
    T Sasaoka
    Department of Medicine, University of California, San Diego, La Jolla 92093
    J Biol Chem 269:13689-94. 1994
  9. ncbi X-ray crystallographic studies on hexameric insulins in the presence of helix-stabilizing agents, thiocyanate, methylparaben, and phenol
    J L Whittingham
    Department of Chemistry, University of York, Heslington, England
    Biochemistry 34:15553-63. 1995
  10. doi Interplay of adiponectin, TNFα and insulin on gene expression, glucose uptake and PPARγ, AKT and TOR pathways in rainbow trout cultured adipocytes
    Marta Bou
    Department of Physiology and Immunology, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain
    Gen Comp Endocrinol 205:218-25. 2014

Scientific Experts

  • Richard B Greaves
  • De Wu
  • Zhi Song Qiao
  • Martial Boutchueng-Djidjou
  • Marta Bou
  • George P Lisi
  • Xiao Dan Liu
  • Simone Renner
  • Yang Li
  • Viktoria Babenko
  • J G M Wientjes
  • Eckhard Wolf
  • Nadja Herbach
  • J William Langston
  • Kun Huang
  • Marjeta Grzech
  • C B Reynolds
  • Shuang Juan Yang
  • Bin Xu
  • Wakari Iwai
  • W De
  • Anne Sophie Van Laere
  • Zhao Jun Zhang
  • YueHua Tang
  • You Min Feng
  • Zhan Yun Guo
  • George Harb
  • Isabelle Kelly
  • Carine Nezer
  • Christian R Landry
  • Michel Georges
  • Minh Nguyen
  • Robert L Faure
  • Sébastien S Hébert
  • Gabriel Collard-Simard
  • Martin Braunschweig
  • Suzanne Fortier
  • Leif Andersson
  • Giuseppe Pandini
  • Kui Li
  • Joaquim Gutierrez
  • Julia Rodriguez
  • Valerie Amarger
  • Jun Hua Fan
  • Encarnacion Capilla
  • Marijana Todorcević
  • Dean E Wilcox
  • Chien Yi M Png
  • Ji Han Xia
  • Isabel Navarro
  • Shu Lin Yang
  • Jin Xue Ruan
  • Barbara Kessler
  • Stefan Krebs
  • Ruediger Wanke
  • Hiroshi Nagashima
  • Yang Liu
  • Christina Braun-Reichhart
  • Nikolai Klymiuk
  • Andrea Bähr
  • Helmut Blum
  • Oliver Puk
  • Chen Wang
  • Mayuko Kurome
  • Andreas Blutke
  • Wojciech Dzwolak
  • Annegret Wünsch
  • Lianqi Huang
  • Jochen Graw
  • Xin Yang
  • Ling Zheng
  • Hao Gong
  • Elisabeth Streckel
  • Daniela Emrich
  • Yue Sun
  • H Van Den Brand
  • F Aarsse
  • B F A Laurenssen
  • R E Koopmanschap
  • N M Soede
  • B Kemp
  • Tak Yee Aw
  • Lynn Harrison
  • Wei Li
  • Cheng Quan Xu
  • Jiang Wei Wu
  • Felix A Habermann
  • Ingrid Renner-Müller
  • Marlon R Schneider
  • C S Whisnant

Detail Information

Publications34

  1. pmc The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network
    Martial Boutchueng-Djidjou
    From the Département de Pédiatrie, Laboratoire de Biologie Cellulaire
    Mol Cell Proteomics 14:1079-92. 2015
    b>Insulin is internalized with its cognate receptor into the endosomal apparatus rapidly after binding to hepatocytes...
  2. doi Amino acid sequence determinants in self-assembly of insulin chiral amyloid superstructures: role of C-terminus of B-chain in association of fibrils
    Viktoria Babenko
    Department of Chemistry, University of Warsaw, Pasteura 1, 02 093 Warsaw, Poland
    FEBS Lett 587:625-30. 2013
    ..amyloid superstructures is a newly recognised phenomenon observed upon agitation-assisted fibrillation of bovine insulin. Here, by surveying several amyloidogenic precursors we examine whether formation of such entities is unique to ..
  3. doi Thermodynamic contributions to the stability of the insulin hexamer
    George P Lisi
    Department of Chemistry, Dartmouth College, 6128 Burke Laboratory, Hanover, New Hampshire 03755, United States
    Biochemistry 53:3576-84. 2014
    The insulin hexamer is resistant to degradation and fibrillation, which makes it an important quaternary structure for its in vivo storage in Zn(2+)- and Ca(2+)-rich vesicles in the pancreas and for pharmaceutical formulations...
  4. doi The study of regulatory effects of Pdx-1, MafA and NeuroD1 on the activity of porcine insulin promoter and the expression of human islet amyloid polypeptide
    Xiao Dan Liu
    Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, People s Republic of China
    Mol Cell Biochem 394:59-66. 2014
    The purpose of the present study was to determine the activation of porcine insulin promoter (PIP) by three transcription factors: pancreatic and duodenal homeobox 1 (Pdx-1), v-maf musculoaponeurotic fibrosarcoma oncogene (MafA) and ..
  5. ncbi Insulin/insulin-like growth factor I hybrid receptors have different biological characteristics depending on the insulin receptor isoform involved
    Giuseppe Pandini
    Istituto di Medicina Interna, Malattie Endocrine e del Metabolismo, University of Catania, Ospedale Garibaldi, 95123 Catania, Italy
    J Biol Chem 277:39684-95. 2002
    The insulin receptor (IR) and the insulin-like growth factor I receptor (IGF-IR) have a highly homologous structure, but different biological effects...
  6. ncbi Cultured trout liver cells: utilization of substrates and response to hormones
    H Segner
    Department of Zoology II, University of Karlsruhe, FRG
    In Vitro Cell Dev Biol Anim 30:306-11. 1994
    ..dihydroxyacetone, and glycerol for glucose and lactate formation; b) the effects of the pancreatic hormones insulin and glucagon on cellular glucose formation, lactate formation, and fatty acid synthesis; and c) the effects of ..
  7. ncbi Growth hormone, interferon-gamma, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1
    L S Argetsinger
    Department of Physiology, University of Michigan Medical School, Ann Arbor 48109 0622, USA
    J Biol Chem 270:14685-92. 1995
    ..In the present study, GH is shown to stimulate tyrosyl phosphorylation of insulin receptor substrate 1 (IRS-1), the principle substrate of the insulin receptor...
  8. ncbi Evidence for a functional role of Shc proteins in mitogenic signaling induced by insulin, insulin-like growth factor-1, and epidermal growth factor
    T Sasaoka
    Department of Medicine, University of California, San Diego, La Jolla 92093
    J Biol Chem 269:13689-94. 1994
    Shc proteins contain a single SH2 domain, lack catalytic activity, and are substrates for activated receptors for insulin, insulin-like growth factor-1 (IGF-1), and epidermal growth factor (EGF)...
  9. ncbi X-ray crystallographic studies on hexameric insulins in the presence of helix-stabilizing agents, thiocyanate, methylparaben, and phenol
    J L Whittingham
    Department of Chemistry, University of York, Heslington, England
    Biochemistry 34:15553-63. 1995
    Three X-ray crystallographic studies have been carried out on pig insulin in the presence of three ligands, thiocyanate, methylparaben (methyl p-hydroxybenzoate), and phenol...
  10. doi Interplay of adiponectin, TNFα and insulin on gene expression, glucose uptake and PPARγ, AKT and TOR pathways in rainbow trout cultured adipocytes
    Marta Bou
    Department of Physiology and Immunology, Faculty of Biology, University of Barcelona, Barcelona 08028, Spain
    Gen Comp Endocrinol 205:218-25. 2014
    ..trout adipocytes, obtained from visceral adipose tissue, the interplay of the adiponectin system, TNFα and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPARγ, as well as on the ..
  11. ncbi Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose
    L Ellis
    Cell 45:721-32. 1986
    b>Insulin stimulates the autophosphorylation of tyrosine residues of the beta subunit of the insulin receptor (IR); this modified insulin-independent kinase has increased activity toward exogenous substrates in vitro...
  12. ncbi Both type I and II insulin-like growth factor receptor binding increase during lactogenesis in bovine mammary tissue
    M H Dehoff
    Department of Dairy Science, University of Florida, Gainesville 32611
    Endocrinology 122:2412-7. 1988
    Bovine GH is a potent stimulant of lactation, and the insulin-like growth factors I and II (IGF-I and -II) are believed to mediate GH's growth-promoting actions...
  13. doi The effect of exposing a critical hydrophobic patch on amyloidogenicity and fibril structure of insulin
    Yang Li
    Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China
    Biochem Biophys Res Commun 440:56-61. 2013
    ..In this study, insulin was used as a model to investigate the effect of exposing a critical hydrophobic patch on amyloidogenicity and ..
  14. ncbi The structure of 2Zn pig insulin crystals at 1.5 A resolution
    E N Baker
    Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand
    Philos Trans R Soc Lond B Biol Sci 319:369-456. 1988
    The paper describes the arrangement of the atoms within rhombohedral crystals of 2Zn pig insulin as seen in electron density maps calculated from X-ray data extending to 1...
  15. pmc Permanent neonatal diabetes in INS(C94Y) transgenic pigs
    Simone Renner
    Chair for Molecular Animal Breeding and Biotechnology, Ludwig Maximilian University Munich, Munich, Germany
    Diabetes 62:1505-11. 2013
    Mutations in the insulin (INS) gene may cause permanent neonatal diabetes mellitus (PNDM). Ins2 mutant mouse models provided important insights into the disease mechanisms of PNDM but have limitations for translational research...
  16. pmc Activation of promoter activity of the catalytic subunit of γ-glutamylcysteine ligase (GCL) in brain endothelial cells by insulin requires antioxidant response element 4 and altered glycemic status: implication for GCL expression and GSH synthesis
    J William Langston
    Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center at Shreveport, Shreveport, LA 71130, USA
    Free Radic Biol Med 51:1749-57. 2011
    Our recent finding that insulin increased the expression of the glutamate-cysteine ligase catalytic subunit (GCLc) with coincident increases in GCL activity and cellular glutathione (GSH) in human brain microvascular endothelial cells (..
  17. doi Effects of dietary carbohydrate sources on plasma glucose, insulin and IGF-I levels in multiparous sows
    J G M Wientjes
    Department of Animal Sciences, Adaptation Physiology Group, Wageningen University, Wageningen, The Netherlands
    J Anim Physiol Anim Nutr (Berl) 96:494-505. 2012
    Effects of different carbohydrate sources on plasma glucose, insulin and insulin-like growth factor-I (IGF-I) levels were compared to subsequently be able to study effects of insulin-stimulating diets on follicle development in sows...
  18. ncbi Acute exposure to streptozotocin but not human proinflammatory cytokines impairs neonatal porcine islet insulin secretion in vitro but not in vivo
    George Harb
    Department of Surgery, University of Alberta, Edmonton, Canada
    Xenotransplantation 14:580-90. 2007
    ..Therefore, we also examined NPI function after acute oxidative stress caused by streptozotocin (STZ), a nitric oxide-generating beta cell cytotoxin...
  19. doi SOCS3 inhibits insulin signaling in porcine primary adipocytes
    Shuang Juan Yang
    Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A and F University, 22 Xinong Road, Yangling, 712100, Shaanxi Province, People s Republic of China
    Mol Cell Biochem 345:45-52. 2010
    b>Insulin resistance is a major player in the pathogenesis of type II diabetes, the metabolic syndrome, and obesity. SOCS3 plays an important role in the development of insulin resistance...
  20. doi Effects of feeding pattern on ghrelin and insulin secretion in pigs
    C B Reynolds
    Department of Animal Science, North Carolina State University, Raleigh, Raleigh, NC 27695 7621, USA
    Domest Anim Endocrinol 39:90-6. 2010
    ..Plasma concentrations of ghrelin and insulin were determined by radioimmunoassay...
  21. pmc A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution
    Wakari Iwai
    Graduate School of Science and Engineering, Ibaraki University, Hitachi, Ibaraki 316 8511, Japan
    Acta Crystallogr D Biol Crystallogr 65:1042-50. 2009
    Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor...
  22. doi Specific transgene expression in mouse pancreatic beta-cells under the control of the porcine insulin promoter
    Marjeta Grzech
    Chair for Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Feodor Lynen Str 25, 81377 Munich, Germany
    Mol Cell Endocrinol 315:219-24. 2010
    ..The rat insulin 2 gene (Ins2) promoter, widely used to achieve transgene expression in pancreatic beta-cells of mice, also ..
  23. pmc Decoding the cryptic active conformation of a protein by synthetic photoscanning: insulin inserts a detachable arm between receptor domains
    Bin Xu
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 284:14597-608. 2009
    ..A model is provided by insulin, a vertebrate hormone central to the control of metabolism...
  24. doi Effect of feeding allowance level on embryonic survival, IGF-1, insulin, GH, leptin and progesterone secretion in early pregnancy gilts
    W De
    Animal Nutrition Institute of Sichuan Agricultural University, Sichuan, Ya an, China
    J Anim Physiol Anim Nutr (Berl) 93:577-85. 2009
    ..Concentrations of insulin-like growth factor I (IGF)-1, insulin, growth harmone (GH), leptin and progesterone in serum and uterine ..
  25. ncbi Contribution of the conserved A16Leu to insulin foldability
    Zhao Jun Zhang
    Institute of Molecular Biology, College of Life Sciences, Nan Kai University, Tian Jin 300071, China
    Protein J 27:192-6. 2008
    Contributions of the evolutionarily conserved A16Leu and B17Leu to insulin foldability were characterized by evaluating folding properties of single-chain insulin analogs...
  26. ncbi Structure of porcine insulin cocrystallized with clupeine Z
    P Balschmidt
    Novo Nordisk A S, Gentofte, Denmark
    Acta Crystallogr B 47:975-86. 1991
    The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods...
  27. ncbi In vitro insulin refolding: characterization of the intermediates and the putative folding pathway
    YueHua Tang
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
    Sci China C Life Sci 50:717-25. 2007
    The in vitro refolding process of the double-chain insulin was studied based on the investigation of in vitro single-chain insulin refolding...
  28. ncbi In vitro folding/unfolding of insulin/single-chain insulin
    Zhi Song Qiao
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Protein Pept Lett 13:423-9. 2006
    ..the in vitro oxidative folding process of two single-chain insulins: porcine insulin precursor (PIP) and human proinsulin (HPI)...
  29. ncbi Altered ionization of the B13 Glu in insulin B9 and B10 mutants: a computational analysis
    Richard B Greaves
    Department of Biology, University of York, York YO10 5YW, UK
    Protein Eng Des Sel 17:557-63. 2004
    ..50 units has been reported for the B13 Glu residue in a dimeric B9 Ser --> Asp insulin mutant relative to the native dimer...
  30. ncbi Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) insulin at 1.65 A resolution
    J S Diao
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Acta Crystallogr D Biol Crystallogr 53:507-12. 1997
    b>Insulin has a concentration of 10(-8)-10(-11) M in the blood which ensures that it circulates and exerts its physiological functions in vivo as a monomer...
  31. ncbi A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig
    Anne Sophie Van Laere
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala University, BMC, Box 597, SE 751 24 Uppsala, Sweden
    Nature 425:832-6. 2003
    ..paternally expressed QTL affecting muscle growth, fat deposition and size of the heart in pigs maps to the IGF2 (insulin-like growth factor 2) region...
  32. ncbi Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs
    Valerie Amarger
    Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, SE 751 24 Uppsala, Sweden
    Mamm Genome 13:388-98. 2002
    ..Here we report two sequence contigs together comprising almost 90 kb containing the INS-IGF2 and H19 genes...
  33. ncbi Porcine proinsulin: characterization and amino acid sequence
    R E Chance
    Science 161:165-7. 1968
    b>Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies...
  34. ncbi Structure of the pig insulin dimer in the cubic crystal
    J Badger
    Department of Physics, University of York, England
    Acta Crystallogr B 47:127-36. 1991
    Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1...