P2X2

Summary

Gene Symbol: P2X2
Description: purinergic receptor P2X 2
Alias: P2X2, P2X purinoceptor 2, ATP receptor, purinergic receptor P2X, ligand-gated ion channel, 2
Species: rat

Top Publications

  1. pmc Characterization of novel Pannexin 1 isoforms from rat pituitary cells and their association with ATP-gated P2X channels
    Shuo Li
    Section on Cellular Signaling, Program in Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892 4510, USA
    Gen Comp Endocrinol 174:202-10. 2011
  2. ncbi On the role of the first transmembrane domain in cation permeability and flux of the ATP-gated P2X2 receptor
    Damien S K Samways
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 283:5110-7. 2008
  3. ncbi New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor
    A J Brake
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Nature 371:519-23. 1994
  4. pmc Regulation of the desensitization and ion selectivity of ATP-gated P2X2 channels by phosphoinositides
    Yuichiro Fujiwara
    Department of Molecular Physiology, Division of Biophysics and Neurobiology, National Institute for Physiological Sciences, Aichi 444 8585, Japan
    J Physiol 576:135-49. 2006
  5. pmc Dynamic aspects of functional regulation of the ATP receptor channel P2X2
    Yoshihiro Kubo
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Physiol 587:5317-24. 2009
  6. ncbi Distinct Localization of P2X receptors at excitatory postsynaptic specializations
    M E Rubio
    Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, D 37075 Gottingen, Germany
    J Neurosci 21:641-53. 2001
  7. pmc Polar residues in the second transmembrane domain of the rat P2X2 receptor that affect spontaneous gating, unitary conductance, and rectification
    Lishuang Cao
    Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PL, UK
    J Neurosci 29:14257-64. 2009
  8. ncbi Expression level dependent changes in the properties of P2X2 receptors
    J D Clyne
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 3095 Natural Science Building, 830 N University Avenue, Ann Arbor, MI 48109 1048, USA
    Neuropharmacology 44:403-12. 2003
  9. pmc A putative extracellular salt bridge at the subunit interface contributes to the ion channel function of the ATP-gated P2X2 receptor
    Ruotian Jiang
    Laboratoire de Biophysicochimie des Récepteurs Canaux, UMR 7199 CNRS, Conception et Application de Molécules Bioactives, Faculte de Pharmacie, Universite de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France
    J Biol Chem 285:15805-15. 2010
  10. pmc An intersubunit zinc binding site in rat P2X2 receptors
    Naomi Nagaya
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 280:25982-93. 2005

Scientific Experts

  • Batu Keceli
  • Shuo Li
  • Yoshihiro Kubo
  • Luciano Moffatt
  • Yuichiro Fujiwara
  • K Nakazawa
  • Yusuke Ishida
  • T Robson
  • C Allgaier
  • Mark T Young
  • Toshio Ohta
  • Masato Fukui
  • M E Rubio
  • Alexander V Gourine
  • Eric Boué-Grabot
  • B S Khakh
  • Guilin Li
  • Yun Gao
  • Shuangmei Liu
  • Shangdong Liang
  • Changshui Xu
  • Jiari Lin
  • Keisuke Migita
  • Zhiyuan Li
  • Kazuhiro Mio
  • Nelson P Barrera
  • R Alan North
  • J Michael Edwardson
  • Richard I Hume
  • Cleber A Trujillo
  • Michel Boris Emerit
  • Damien S K Samways
  • Yuwei Liu
  • Rachel K Tittle
  • Ligia S Antonio
  • Olga Stelmashenko
  • Xin Liang
  • Ruibiao Yang
  • Jun Xu
  • Sukanya Punthambaker
  • Chiara Mangano
  • Philip M Dunn
  • Christian Wolf
  • Geoffrey Burnstock
  • Ariane Silva Paulino
  • Esther Richler
  • Lalah M Niane
  • Telma T Schwindt
  • Han Liu
  • Patricia Castelucci
  • Zhi Wang Li
  • Marcia Sanae Mizuno
  • Edson Aparecido Liberti
  • Priscila Azevedo Girotti
  • Kazunori Adachi
  • Jun Zhang
  • Rúbia Misawa
  • Maria Cláudia Gonçalves de Oliveira Fusaro
  • G Colldén
  • Ruotian Jiang
  • Xin Li
  • Kehua Yu
  • Yunxia Wang
  • Lishuang Cao
  • Dayane A Gomes
  • Henning Ulrich
  • Khoa Dang
  • Shuichiro Kakimoto
  • Katsuaki Ito
  • Günther Schmalzing
  • Eiji Shigetomi
  • Toshihiko Ogura
  • Tomomi Yamamoto
  • Chikara Sato
  • Denise Greenwood
  • Terrance M Egan
  • Jialie Luo
  • Ruth D Murrell-Lagnado
  • Robert M Henderson
  • Marianela Masin
  • Ricardo J Rodrigues
  • Ramón A Lorca
  • Simon Studeny
  • Peter R Thorne
  • Naomi Nagaya
  • Gary D Housley
  • Allen F Ryan
  • Huai Zhen Ruan
  • Bei Ma
  • Severine Chaumont

Detail Information

Publications77

  1. pmc Characterization of novel Pannexin 1 isoforms from rat pituitary cells and their association with ATP-gated P2X channels
    Shuo Li
    Section on Cellular Signaling, Program in Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892 4510, USA
    Gen Comp Endocrinol 174:202-10. 2011
    ..These splice forms can also physically associate with ATP-gated P2X2, P2X3, P2X4, and P2X7 receptor channels...
  2. ncbi On the role of the first transmembrane domain in cation permeability and flux of the ATP-gated P2X2 receptor
    Damien S K Samways
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 283:5110-7. 2008
    ..mutagenesis and patch clamp photometry to study the role of the first transmembrane domain of the rat P2X2 receptor in cation permeability and flux...
  3. ncbi New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor
    A J Brake
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Nature 371:519-23. 1994
    ..There is sequence similarity between the ATP receptor and RP-2, a gene activated in thymocytes undergoing programmed cell death...
  4. pmc Regulation of the desensitization and ion selectivity of ATP-gated P2X2 channels by phosphoinositides
    Yuichiro Fujiwara
    Department of Molecular Physiology, Division of Biophysics and Neurobiology, National Institute for Physiological Sciences, Aichi 444 8585, Japan
    J Physiol 576:135-49. 2006
    ....
  5. pmc Dynamic aspects of functional regulation of the ATP receptor channel P2X2
    Yoshihiro Kubo
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Physiol 587:5317-24. 2009
    ..Complete elucidation of these regulatory mechanisms awaits dynamic imaging of functioning P2X(2) channels...
  6. ncbi Distinct Localization of P2X receptors at excitatory postsynaptic specializations
    M E Rubio
    Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, D 37075 Gottingen, Germany
    J Neurosci 21:641-53. 2001
    ..The present study shows for the first time qualitatively and quantitatively the precise localization of P2X receptors in brain. Moreover, our data indicate that P2X receptors may play a significant role at glutamatergic synapses...
  7. pmc Polar residues in the second transmembrane domain of the rat P2X2 receptor that affect spontaneous gating, unitary conductance, and rectification
    Lishuang Cao
    Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PL, UK
    J Neurosci 29:14257-64. 2009
    ..We have measured single-channel currents, spontaneous gating, and rectification of rat P2X2 receptor in which polar and charged residues of the second transmembrane domain (TM2) were systematically probed by ..
  8. ncbi Expression level dependent changes in the properties of P2X2 receptors
    J D Clyne
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 3095 Natural Science Building, 830 N University Avenue, Ann Arbor, MI 48109 1048, USA
    Neuropharmacology 44:403-12. 2003
    ..Thus, the interactions that cause expression-dependent changes in P2X(2) receptor properties must involve domains proximal to position H397...
  9. pmc A putative extracellular salt bridge at the subunit interface contributes to the ion channel function of the ATP-gated P2X2 receptor
    Ruotian Jiang
    Laboratoire de Biophysicochimie des Récepteurs Canaux, UMR 7199 CNRS, Conception et Application de Molécules Bioactives, Faculte de Pharmacie, Universite de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France
    J Biol Chem 285:15805-15. 2010
    ..By using a P2X2 model based on the x-ray structure, we sought salt bridges formed between charged residues located in a region that ..
  10. pmc An intersubunit zinc binding site in rat P2X2 receptors
    Naomi Nagaya
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 280:25982-93. 2005
    ..Zinc binds to rat P2X2 receptors and acts as an allosteric modulator, potentiating channel opening...
  11. ncbi Gain and loss of channel function by alanine substitutions in the transmembrane segments of the rat ATP-gated P2X2 receptor
    Zhiyuan Li
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Neurosci 24:7378-86. 2004
    ATP opens ionotropic P2X channels through a process that is poorly understood. We made an array of mutant rat P2X2 channels containing unique alanine substitutions in the transmembrane segments with the goal of identifying possible ..
  12. pmc Density-dependent changes of the pore properties of the P2X2 receptor channel
    Yuichiro Fujiwara
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University Graduate School and Faculty of Medicine, Japan
    J Physiol 558:31-43. 2004
    ..One exception is the P2X ATP-gated channel. After application of ATP, the ion selectivity of the P2X2 channel time-dependently changes, i.e...
  13. doi P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice
    Yusuke Ishida
    Department of Neurobiology and Anatomy, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467 8601, Japan
    J Comp Neurol 514:131-44. 2009
    ..Taken together, these findings suggest that most P2X(2) and P2X(3) receptors in fungiform papillae are used for gustation rather than somatosensation...
  14. doi A New Mechanism of Receptor Targeting by Interaction between Two Classes of Ligand-Gated Ion Channels
    Michel Boris Emerit
    Centre de psychiatrie et Neurosciences, Inserm UMR 894, 75013 Paris, France, Universite Paris V, 75006 Paris, France
    J Neurosci 36:1456-70. 2016
    The 5-HT3 receptors are serotonin-gated ion channels that physically couple with purinergic P2X2 receptors to trigger a functional cross-inhibition leading to reciprocal channel occlusion...
  15. pmc Ectodomain movements of an ATP-gated ion channel (P2X2 receptor) probed by disulfide locking
    Olga Stelmashenko
    From the Faculty of Medical and Human Sciences, and
    J Biol Chem 289:9909-17. 2014
    ..In the present work, we examined this opening model by introducing pairs of cysteines into the rat P2X2 receptor that might form disulfide bonds within or between subunits...
  16. pmc Functional identification of close proximity amino acid side chains within the transmembrane-spanning helixes of the P2X2 receptor
    Xin Liang
    Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, University of Chinese Academy of Sciences, Guangzhou, Guangdong, China
    PLoS ONE 8:e70629. 2013
    ..The relative movement between the first transmembrane and the second transmembrane in the intra-subunit is likely important for transmitting the action of ATP binding to the opening of the channel...
  17. doi Identification of P2X2/P2X4/P2X6 heterotrimeric receptors using atomic force microscopy (AFM) imaging
    Ligia S Antonio
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
    FEBS Lett 588:2125-8. 2014
    Seven P2X purinergic receptor subunits have been identified: P2X1-P2X7. The overlapping expression of P2X2, P2X4 and P2X6 subunits has been shown in different cell types, and functional analysis of P2X receptors in Leydig cells suggests ..
  18. pmc Signal transmission within the P2X2 trimeric receptor
    Batu Keceli
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Aichi 444 8585, Japan
    J Gen Physiol 143:761-82. 2014
    b>P2X2 receptor channel, a homotrimer activated by the binding of extracellular adenosine triphosphate (ATP) to three intersubunit ATP-binding sites (each located ∼50 Å from the ion permeation pore), also shows voltage-dependent ..
  19. pmc Voltage- and ATP-dependent structural rearrangements of the P2X2 receptor associated with the gating of the pore
    Batu Keceli
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, 444 8585, Japan
    J Physiol 592:4657-76. 2014
    b>P2X2 is an extracellular ATP-gated cation channel which has a voltage-dependent gating property even though it lacks a canonical voltage sensor...
  20. doi Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp
    Yuwei Liu
    Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, Hubei Province, China School of Medicine, Jianghan University, Wuhan, 430056, China
    Biochem Biophys Res Commun 458:596-600. 2015
    ..to ATP with the type F current mainly showed positive staining for P2X3 and P2X5, but negative staining for P2X2; cells responsive to ATP with the type I current showed positive staining for P2X1-3 and P2X5, but negative ..
  21. pmc Immunocytochemical analysis of P2X2 in rat circumvallate taste buds
    Ruibiao Yang
    Department of Biological Sciences, University of Denver, Denver, CO 80208, USA
    BMC Neurosci 13:51. 2012
    ..Recent studies indicate that the ionotropic purinergic receptors P2X2/P2X3 are present in rodent taste buds...
  22. pmc High potency zinc modulation of human P2X2 receptors and low potency zinc modulation of rat P2X2 receptors share a common molecular mechanism
    Sukanya Punthambaker
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 287:22099-111. 2012
    Human P2X2 receptors (hP2X2) are strongly inhibited by zinc over the range of 2-100 μM, whereas rat P2X2 receptors (rP2X2) are strongly potentiated over the same range, and then inhibited by zinc over 100 μM...
  23. pmc Chemical phenotypes of P2X2 purinoreceptor immunoreactive cell bodies in the area postrema
    Chiara Mangano
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Purinergic Signal 8:223-34. 2012
    ..The purinergic receptor subunit P2X2 is a ligand-gated ion channel that is widely expressed by neurons in the CNS...
  24. doi Spontaneous firing and evoked responses of spinal nociceptive neurons are attenuated by blockade of P2X3 and P2X2/3 receptors in inflamed rats
    Jun Xu
    Neuroscience Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064 6118, USA
    J Neurosci Res 90:1597-606. 2012
    P2X3 and P2X2/3 receptors are selectively expressed on primary afferent nociceptors and have been implicated in modulating nociception in different models of pathological pain, including inflammatory pain...
  25. pmc Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated
    Esther Richler
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, USA
    J Neurosci 31:16716-30. 2011
    ATP-gated ionotropic P2X2 receptors are widely expressed in neurons...
  26. doi Expressions of P2X2 and P2X3 receptors in rat nodose neurons after myocardial ischemia injury
    Yunxia Wang
    Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, PR China
    Auton Neurosci 145:71-5. 2009
    ..It suggests that P2X(2) receptor subtype in company with P2X(3) receptor subtype plays the important role in cardiac vagal sensory nociceptors with a sensitivity to ATP...
  27. ncbi A histidine scan to probe the flexibility of the rat P2X2 receptor zinc-binding site
    Rachel K Tittle
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 1048, USA
    J Biol Chem 282:19526-33. 2007
    ..Our data are consistent with a moderately flexible zinc-binding site and inconsistent with parallel and anti-parallel orientations of the regions surrounding histidines 120 and 213...
  28. doi Reconstruction of the P2X(2) receptor reveals a vase-shaped structure with lateral tunnels above the membrane
    Kazuhiro Mio
    Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, Umezono 1 1 4, Tsukuba, Ibaraki 305 8568, Japan
    Structure 17:266-75. 2009
    ..The sparse, extended structure of the P2X(2) receptor indicates a loose assembly of subunits, which could be a basis for the activation-dependent pore dilation of P2X receptors...
  29. pmc Role of the domain encompassing Arg304-Ile328 in rat P2X2 receptor conformation revealed by alterations in complex glycosylation at Asn298
    Mark T Young
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Manchester, UK
    Biochem J 416:137-43. 2008
    ....
  30. doi Modulation of P2X receptors in dorsal root ganglion neurons of streptozotocin-induced diabetic neuropathy
    Keisuke Migita
    Department of Neurophysiology, Hirosaki University Graduate School of Medicine, 5 Zaifu cho, Hirosaki, Aomori 036 8562, Japan
    Neurosci Lett 452:200-3. 2009
    ..These results suggest that the upregulation of P2X(2), P2X(3) and/or P2X(2/3) receptor in DRG neurons is associated with mechanical allodynia in STZ-induced diabetic mice...
  31. doi Minodronic acid, a third-generation bisphosphonate, antagonizes purinergic P2X(2/3) receptor function and exerts an analgesic effect in pain models
    Shuichiro Kakimoto
    Drug Discovery Research, Astellas Pharma Inc, Tsukuba, Ibaraki, Japan
    Eur J Pharmacol 589:98-101. 2008
    ..These unique effects of minodronic acid would be beneficial for the treatment of accelerated bone turnover diseases accompanied by bone pain, including bone metastases...
  32. pmc Sustained stimulation of vasopressin and oxytocin release by ATP and phenylephrine requires recruitment of desensitization-resistant P2X purinergic receptors
    Dayane A Gomes
    Department of Physiology and Biophysics, University of Colorado Denver School of Medicine, Denver, Colorado 80045, USA
    Am J Physiol Regul Integr Comp Physiol 297:R940-9. 2009
    ..by recruitment of desensitization-resistant ionotropic purinergic receptor subtypes (P2X-Rs): 1) Antagonists of the P2X2/3 and P2X7-Rs truncated the sustained VP/OT release induced by ATP+PE but did not alter the transient response to ..
  33. doi Increased sympathoexcitatory reflex induced by myocardial ischemic nociceptive signaling via P2X2/3 receptor in rat superior cervical ganglia
    Guilin Li
    Department of Physiology, Basic Medical College of Nanchang University, Bayi Road 461, Nanchang 330006, PR China
    Neurochem Int 56:984-90. 2010
    ..The myocardial ischemic injury induced an increase in the expression of P2X(2/3) receptor colocalization with TH and a hypersensitivity state of SCG neurons to ATP, which led to the increased sympathoexcitatory reflex...
  34. doi Directed differentiation of neural progenitors into neurons is accompanied by altered expression of P2X purinergic receptors
    Telma T Schwindt
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Av Prof Lineu Prestes, Brazil
    J Mol Neurosci 44:141-6. 2011
    ..rat neurospheres cultured in suspension under mitogen-free condition presented significant higher expression of P2X2 and P2X6 receptor subunits, when compared to cells cultured in the presence of growth factors, suggesting a direct ..
  35. pmc Effects of protein deprivation and re-feeding on P2X2 receptors in enteric neurons
    Rúbia Misawa
    Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof Dr Lineu Prestes 2415, Sao Paulo, Brazil
    World J Gastroenterol 16:3651-63. 2010
    ..To investigate the effects of malnutrition and re-feeding on the P2X(2) receptor, nitric oxide synthase (NOS), calretinin, calbindin and choline acetyltransferase (ChAT) in neurons of the rat ileum...
  36. doi VEGF and its receptor-2 involved in neuropathic pain transmission mediated by P2X₂(/)₃ receptor of primary sensory neurons
    Jiari Lin
    Department of Physiology, Medical College of Nanchang University, Jiangxi, PR China
    Brain Res Bull 83:284-91. 2010
    ..Anti-rVEGF treatment in CCI rats may alleviate chronic neuropathic pain by decreasing the expressions of VEGFR2 and P2X₂(/)₃ receptors on DRG neurons to inhibit the transmission of neuropathic pain signaling...
  37. doi P2X2 purinoreceptor protein in hypothalamic neurons associated with the regulation of food intake
    G Colldén
    Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    Neuroscience 171:62-78. 2010
    ..The purinergic receptor subunit P2X2 is a ligand-gated ion channel that is widely expressed by neurons in the central nervous system...
  38. pmc Purinergic receptors are involved in tooth-pulp evoked nocifensive behavior and brainstem neuronal activity
    Kazunori Adachi
    Department of Physiology, Nihon University School of Dentistry, Nihon University School of Dentistry, 1 8 13 Kanda Surugadai Chiyoda ku, Tokyo, 101 8310, Japan
    Mol Pain 6:59. 2010
    To evaluate whether P2X receptors are involved in responses to noxious pulp stimulation, the P2X3 and P2X2/3 receptor agonist α,β-methyleneATP (α,β-meATP) was applied to the molar tooth pulp and nocifensive behavior and extracellular-..
  39. doi P2X3 and P2X2/3 receptors mediate mechanical hyperalgesia induced by bradykinin, but not by pro-inflammatory cytokines, PGE₂ or dopamine
    Maria Cláudia Gonçalves de Oliveira Fusaro
    Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas UNICAMP, Limeira Av 901, 13414 900 Piracicaba, Sao Paulo, Brazil
    Eur J Pharmacol 649:177-82. 2010
    Activation of peripheral P2X3 and P2X2/3 receptors by endogenous ATP is essential to the development of inflammatory hyperalgesia...
  40. pmc Ventilatory and carotid body chemoreceptor responses to purinergic P2X receptor antagonists in newborn rats
    Lalah M Niane
    Unité de Recherche en Périnatologie, Centre Hospitalier Universitaire de Quebec, Hopital Saint Francois d Assise, Departement de Pediatrie, Universite Laval, Quebec, Canada
    J Appl Physiol (1985) 110:83-94. 2011
    ..However, these effects are not age dependent within the age range studied...
  41. doi Molecular determinants of potent P2X2 antagonism identified by functional analysis, mutagenesis, and homology docking
    Christian Wolf
    Department of Molecular Pharmacology, RWTH Aachen University, Aachen, Germany
    Mol Pharmacol 79:649-61. 2011
    b>P2X2 receptors are members of the ATP-gated P2X family of cation channels, and they participate in neurotransmission in sympathetic ganglia and interneurons...
  42. doi Effect of emodin on neuropathic pain transmission mediated by P2X2/3 receptor of primary sensory neurons
    Yun Gao
    Department of Physiology, Medical College of Nanchang University, Bayi Road 461, Nanchang, Jiangxi, PR China
    Brain Res Bull 84:406-13. 2011
    ..The results showed that emodin can inhibit the transmission of neuropathic pain mediated by P2X(2/3) receptor of primary sensory neurons to alleviate chronic neuropathic pain...
  43. doi Effects of ischemia and reperfusion on P2X2 receptor expressing neurons of the rat ileum enteric nervous system
    Ariane Silva Paulino
    Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof Dr Lineu Prestes, 2415, CEP 05508 900, Sao Paulo, Brazil
    Dig Dis Sci 56:2262-75. 2011
    We investigated the effects of ischemia/reperfusion in the intestine (I/R-i) on purine receptor P2X2-immunoreactive (IR) neurons of the rat ileum.
  44. ncbi P2X and P2Y purinoceptor expression in pancreas from streptozotocin-diabetic rats
    Robson Coutinho-Silva
    Autonomic Neuroscience Institute, Royal Free and University College London Medical School, Rowland Hill Street, London NW3 2PF, UK
    Mol Cell Endocrinol 204:141-54. 2003
    The expression of the nucleotide receptors P2X1, P2X2, P2X7, P2Y1, P2Y2 and P2Y4, in the pancreas of the streptozotocin-induced diabetic rat was investigated using immunohistochemistry...
  45. pmc Cyclophosphamide-induced bladder inflammation sensitizes and enhances P2X receptor function in rat bladder sensory neurons
    Khoa Dang
    Departments of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA
    J Neurophysiol 99:49-59. 2008
    ....
  46. ncbi Trimeric architecture of homomeric P2X2 and heteromeric P2X1+2 receptor subtypes
    Armaz Aschrafi
    Department of Molecular Pharmacology, Technical University of Aachen, Wendlingweg 2, D 52074, Germany
    J Mol Biol 342:333-43. 2004
    ..evidence that in addition to the rapidly desensitising P2X1 and P2X3 receptors, the slowly desensitising subtypes P2X2, P2X4, and P2X5 are trimers of identical subunits...
  47. ncbi Somatic and axonal effects of ATP via P2X2 but not P2X7 receptors in rat thoracolumbar sympathetic neurones
    C Allgaier
    Rudolf Boehm Institute of Pharmacology and Toxicology, Department of Neurophysiology, University of Leipzig, Leipzig, Germany
    J Neurochem 90:359-67. 2004
    ..However, the functional significance of axonal and (peri)-nuclear P2X(7) receptors has still to be proven...
  48. ncbi Identification of a trafficking motif involved in the stabilization and polarization of P2X receptors
    Severine Chaumont
    Departement de Pharmacologie, Laboratoire de Génomique Fonctionnelle, CNRS UPR2580, 141 rue de la Cardonille, 34396 Montpellier, France
    J Biol Chem 279:29628-38. 2004
    ....
  49. ncbi Identification of P2X receptors in cultured mouse and rat parasympathetic otic ganglion neurones including P2X knockout studies
    Bei Ma
    Autonomic Neuroscience Institute, Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    Neuropharmacology 46:1039-48. 2004
    ..In conclusion, we have shown for the first time that at least two distinct P2X receptors are present in mouse and rat otic neurones, probably homomeric P2X(2) and heteromeric P2X(2/3) receptors...
  50. ncbi Intracellular disulfide bond that affects ATP responsiveness of P2X2 receptor/channel
    Ken Nakazawa
    Division of Pharmacology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya, 158 8501 Tokyo, Japan
    Eur J Pharmacol 474:205-8. 2003
    ..These results suggest that an intracellular disulfide bond involving Cys(9) regulates the responsiveness of P2X(2) receptor/channel to ATP...
  51. pmc Purinergic signalling in the medullary mechanisms of respiratory control in the rat: respiratory neurones express the P2X2 receptor subunit
    Alexander V Gourine
    Department of Physiology, Royal Free and University College London Medical School, Rowland Hill Street, London NW3 2PF, U K
    J Physiol 552:197-211. 2003
    ..In particular we determined whether respiratory neurones in the rostral ventrolateral medulla (VLM) express P2X2 receptor subunits of the ATP-gated ion channel, since ATP currents evoked at recombinant P2X2 receptors are ..
  52. ncbi Noise induces up-regulation of P2X2 receptor subunit of ATP-gated ion channels in the rat cochlea
    Julie C C Wang
    Department of Physiology, University of Auckland, Private Bag 92019, New Zealand
    Neuroreport 14:817-23. 2003
    ....
  53. ncbi Subunit-specific coupling between gamma-aminobutyric acid type A and P2X2 receptor channels
    Eric Boué-Grabot
    Laboratoire de Neurophysiologie, CNRS Unité Miate de Recherche UMR 5543, Universite Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France
    J Biol Chem 279:52517-25. 2004
    ..We report here that co-activation of P2X2 and various GABAA receptors, co-expressed in Xenopus oocytes, leads to a functional cross-inhibition dependent on ..
  54. pmc Dehydroepiandrosterone potentiates native ionotropic ATP receptors containing the P2X2 subunit in rat sensory neurones
    Mathias De Roo
    Laboratoire de Neurophysiologie Cellulaire et Intégrée, UMR 7519 CNRS, Universite Louis Pasteur, 21 rue René Descartes, 67084 Strasbourg Cedex, France
    J Physiol 552:59-71. 2003
    ..3) and by ivermectin (IVM, 3 microM), indicating the presence heteromeric P2X2-containing receptors and possibly of functional native P2X4/6 receptors...
  55. ncbi Differential increases in P2X receptor levels in rat vagal efferent neurones following a vagal nerve section
    Lucy Atkinson
    School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, UK
    Brain Res 977:112-8. 2003
    ..Thus the increase in P2XR levels in DVN cells ipsilateral to a nerve section are likely to reflect an increase in expression of functional P2XRs on the cell surface...
  56. ncbi Cerebellar lesion up-regulates P2X1 and P2X2 purinergic receptors in precerebellar nuclei
    F Florenzano
    I R C C S Santa Lucia Foundation, Via Ardeatina 306, 00179 Rome, Italy
    Neuroscience 115:425-34. 2002
    ..They provide in vivo evidence of a correlation between purinergic receptor subunit up-regulation and survival of injured neurons...
  57. ncbi P2X(2) receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat
    L Atkinson
    School of Biomedical Sciences, University of Leeds, LS2 9NQ, Leeds, UK
    Neuroscience 99:683-96. 2000
    ....
  58. ncbi P2X2 receptor subunit expression in a subpopulation of cochlear type I spiral ganglion neurones
    S G Salih
    Department of Physiology, Faculty of Medicine and Health Science, University of Auckland, New Zealand
    Neuroreport 9:279-82. 1998
    Spiral ganglion neurones in rat cochlea express three different isoforms of the P2X2 receptor subunit which assemble into ATP-gated ion channels. Two of these P2X2R subunit isoforms have previously been detected in other auditory tissues...
  59. ncbi Desensitization of the P2X(2) receptor controlled by alternative splicing
    U Brandle
    Department of Otolaryngology, University of Tubingen, Germany
    FEBS Lett 404:294-8. 1997
    ..These results imply functional differences between cells differentially expressing these P2X(2) isoforms...
  60. ncbi Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea
    G D Housley
    Department of Physiology, University of Auckland, New Zealand
    Biochem Biophys Res Commun 212:501-8. 1995
    ..The region of the receptor lost in the truncated version coded for a number of serine/proline rich regions which may act as potential intracellular regulatory sites...
  61. ncbi Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function
    Marianela Masin
    Department of Molecular Biology of Neuronal Signals, Max Planck Institute for Experimental Medicine, Gottingen, Germany
    J Biol Chem 281:4100-8. 2006
    ..Thus, the time- and activation-dependent change in ionic selectivity of P2X(2) receptors was inhibited by coexpression of Fe65, suggesting a novel role for Fe65 in regulating P2X receptor function and ATP-mediated synaptic transmission...
  62. ncbi Therapeutic effects of the putative P2X3/P2X2/3 antagonist A-317491 on cyclophosphamide-induced cystitis in rats
    Katsuaki Ito
    Department of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889 2192, Japan
    Naunyn Schmiedebergs Arch Pharmacol 377:483-90. 2008
    ..In this study, we investigated the effect of the recently developed P2X3 and P2X2/3 receptor antagonist A-317491 on cyclophosphamide (CYP)-induced cystitis to determine whether a P2X receptor ..
  63. pmc Responses of rat P2X2 receptors to ultrashort pulses of ATP provide insights into ATP binding and channel gating
    Luciano Moffatt
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    J Gen Physiol 130:183-201. 2007
    ..Like NMDA receptors, P2X(2) receptors provide the potential for complex patterns of synaptic integration over a time scale of hundreds of milliseconds...
  64. pmc The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels
    Nelson P Barrera
    Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom
    Biophys J 93:505-12. 2007
    ..We suggest that this property of receptor assembly might introduce an additional layer of subtlety into P2X receptor signaling...
  65. ncbi P2X receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea
    Denise Greenwood
    Department of Physiology, University of Auckland, Private Bag 92019, Auckland, New Zealand
    Development 134:1407-17. 2007
    ..These findings indicate that P2X receptor signaling provides a mechanism for inhibiting neurotrophin support of SGN neurites when synaptic reorganization is occurring in the cochlea...
  66. ncbi Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons
    Jialie Luo
    Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China
    Brain Res 1115:9-15. 2006
    ..current was associated with cell size and P2X receptor subunits: small-sized cells with type F of I(ATP) express only P2X1 and/or P2X3 subunits, while cells with types S and I of I(ATP) express P2X2 or P2X4 in addition to P2X1 and P2X3.
  67. pmc Inhibitory role of supraspinal P2X3/P2X2/3 subtypes on nociception in rats
    Masato Fukui
    Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Mol Pain 2:19. 2006
    ..c.v.) pretreatment with A-317491 (1 nmol), the novel non-nucleotide antagonist selective for P2X3 and P2X2/3 receptors, attenuated the antinociceptive effect produced by i.c.v...
  68. ncbi Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize
    Nelson P Barrera
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Biol Chem 280:10759-65. 2005
    ..We conclude that P2X(2) receptors are trimers, whereas the P2X(6) receptor subunits do not form stable oligomers...
  69. ncbi Visualization of the trimeric P2X2 receptor with a crown-capped extracellular domain
    Kazuhiro Mio
    Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, Umezono 1 1 4, Tsukuba, Ibaraki 305 8568, Japan
    Biochem Biophys Res Commun 337:998-1005. 2005
    The P2X2 purinergic receptor permeates cationic ions in response to stimulation by ATP and mediates fast synaptic transmission...
  70. ncbi Extracellular histidine residues identify common structural determinants in the copper/zinc P2X2 receptor modulation
    Ramón A Lorca
    Departmentos de Fisiología, Centro de Regulacion Celular y Patologia J V Luco, Instituto MIFAB, Faculty de Ciencias biologicas, Pontifica Universidad Catolica de Chile, Santiago, Chile
    J Neurochem 95:499-512. 2005
    To assess the mechanism of P2X2 receptor modulation by transition metals, the cDNA for the wild-type receptor was injected to Xenopus laevis oocytes and examined 48-72 h later by the two-electrode voltage-clamp technique...
  71. ncbi An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy
    Baljit S Khakh
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    J Neurosci 25:6911-20. 2005
    ..Here, we show that plasma membrane alpha4beta2 nicotinic and P2X2 channels form a molecular scale partnership and also influence each other when coactivated, resulting in ..
  72. ncbi Dual presynaptic control by ATP of glutamate release via facilitatory P2X1, P2X2/3, and P2X3 and inhibitory P2Y1, P2Y2, and/or P2Y4 receptors in the rat hippocampus
    Ricardo J Rodrigues
    Centre for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, 3004 504 Coimbra, Portugal
    J Neurosci 25:6286-95. 2005
    ..release of glutamate from purified nerve terminals of the rat hippocampus, the facilitation being mediated by P2X1, P2X2/3, and P2X3 [antagonized by 8-(benzamido)naphthalene-1,3,5-trisulfonate and 2',3'-O-(2,4,6-trinitrophenyl)-ATP] and ..
  73. ncbi Developmental changes in heteromeric P2X(2/3) receptor expression in rat sympathetic ganglion neurons
    Philip M Dunn
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, London, United Kingdom
    Dev Dyn 234:505-11. 2005
    ....
  74. pmc P2X2 and P2X3 receptor expression in postnatal and adult rat urinary bladder and lumbosacral spinal cord
    Simon Studeny
    University of Vermont College of Medicine, Department of Neurology, Burlington, VT 05405, USA
    Am J Physiol Regul Integr Comp Physiol 289:R1155-68. 2005
    ..This may involve changes in neuroactive compounds and receptors in bladder reflex pathways. We examined P2X2 and P2X3 receptors in bladder and spinal cord from postnatal (P0-P36, indicating number of days) and adult Wistar ..
  75. ncbi P2X2 receptors are essential for [Ca2+]i increases in response to ATP in cultured rat myenteric neurons
    Toshio Ohta
    Laboratory of Pharmacology, Dept of Biomedical Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Am J Physiol Gastrointest Liver Physiol 289:G935-48. 2005
    ..RT-PCR and immunocytochemical studies showed the expression of P2X2 receptors in cultured rat myenteric neurons...
  76. ncbi Inhibition mechanism of the recombinant rat P2X(2) receptor in glial cells by suramin and TNP-ATP
    Cleber A Trujillo
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo 05508 900, Brazil
    Biochemistry 45:224-33. 2006
    ..Taken together, both data support a noncompetitive inhibition mechanism of the rat recombinant P2X(2) receptor by suramin, confirm the competitive inhibition by TNP-ATP, and allow the prediction of a model for P2X(2) receptor inhibition...
  77. ncbi Evidence for three additional P2X2 purinoceptor isoforms produced by alternative splicing in the adult rat vestibular end-organs
    M Troyanovskaya
    Department of Otolaryngology, Mount Sinai School of Medicine, New York, NY, USA
    Hear Res 126:201-9. 1998
    b>P2X2 receptors are ligand-gated ion channels that are activated by extracellular ATP...