RAD51

Summary

Gene Symbol: RAD51
Description: recombinase RAD51
Alias: MUT5, recombinase RAD51
Species: Saccharomyces cerevisiae S288c
Products:     RAD51

Top Publications

  1. Ribeyre C, Lopes J, Boulé J, Piazza A, Guédin A, Zakian V, et al. The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo. PLoS Genet. 2009;5:e1000475 pubmed publisher
    ..Hence, we conclude that CEB1 instability in pif1Delta cells depends on the potential to form G-quadruplex structures, suggesting that Pif1 could play a role in the metabolism of G4-forming sequences. ..
  2. Motegi A, Kuntz K, Majeed A, Smith S, Myung K. Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1424-33 pubmed
    ..We propose a mechanism for how defects in these proteins could lead to diverse outcomes (proper repair or GCR formation) through different regulation of DNA repair machinery. ..
  3. Tsubouchi H, Roeder G. The importance of genetic recombination for fidelity of chromosome pairing in meiosis. Dev Cell. 2003;5:915-25 pubmed
    ..Genetic analysis indicates that Hop2 acts in the same pathway as the Rad51 and Dmc1 proteins, two homologs of E. coli RecA...
  4. Oum J, Seong C, Kwon Y, Ji J, Sid A, Ramakrishnan S, et al. RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks. Mol Cell Biol. 2011;31:3924-37 pubmed publisher
    ..This study provides molecular insights into how chromatin remodeling contributes to DNA repair and maintenance of chromatin fidelity in the face of DNA damage. ..
  5. Petukhova G, Sung P, Klein H. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev. 2000;14:2206-15 pubmed
    ..product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase...
  6. Krishna S, Wagener B, Liu H, Lo Y, Sterk R, Petrini J, et al. Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication. DNA Repair (Amst). 2007;6:797-808 pubmed
    ..Interestingly, yku70Delta suppressed BIR in mre11 mutants. BIR is also elevated in rad51 mutants, but yku70Delta did not suppress BIR in a rad51 background...
  7. Davis A, Symington L. The Rad52-Rad59 complex interacts with Rad51 and replication protein A. DNA Repair (Amst). 2003;2:1127-34 pubmed
    ..Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-..
  8. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..Thus, RPA is required to generate ssDNA, and also to protect ssDNA from degradation and inappropriate annealing that could lead to genome rearrangements. ..
  9. Nimonkar A, Dombrowski C, Siino J, Stasiak A, Stasiak A, Kowalczykowski S. Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem. 2012;287:28727-37 pubmed publisher
    ..Dmc1, a meiosis-specific paralog of Rad51, mediates the pairing of homologous chromosomes...
  10. Kelly M, Alver B, Kirkpatrick D. Minisatellite alterations in ZRT1 mutants occur via RAD52-dependent and RAD52-independent mechanisms in quiescent stationary phase yeast cells. DNA Repair (Amst). 2011;10:556-66 pubmed publisher
    ..We propose that the mechanism of ZRT1-mediated minisatellite instability during quiescence is relevant to human cells, and thus, human disease. ..

Detail Information

Publications62

  1. Ribeyre C, Lopes J, Boulé J, Piazza A, Guédin A, Zakian V, et al. The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo. PLoS Genet. 2009;5:e1000475 pubmed publisher
    ..Hence, we conclude that CEB1 instability in pif1Delta cells depends on the potential to form G-quadruplex structures, suggesting that Pif1 could play a role in the metabolism of G4-forming sequences. ..
  2. Motegi A, Kuntz K, Majeed A, Smith S, Myung K. Regulation of gross chromosomal rearrangements by ubiquitin and SUMO ligases in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:1424-33 pubmed
    ..We propose a mechanism for how defects in these proteins could lead to diverse outcomes (proper repair or GCR formation) through different regulation of DNA repair machinery. ..
  3. Tsubouchi H, Roeder G. The importance of genetic recombination for fidelity of chromosome pairing in meiosis. Dev Cell. 2003;5:915-25 pubmed
    ..Genetic analysis indicates that Hop2 acts in the same pathway as the Rad51 and Dmc1 proteins, two homologs of E. coli RecA...
  4. Oum J, Seong C, Kwon Y, Ji J, Sid A, Ramakrishnan S, et al. RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks. Mol Cell Biol. 2011;31:3924-37 pubmed publisher
    ..This study provides molecular insights into how chromatin remodeling contributes to DNA repair and maintenance of chromatin fidelity in the face of DNA damage. ..
  5. Petukhova G, Sung P, Klein H. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev. 2000;14:2206-15 pubmed
    ..product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase...
  6. Krishna S, Wagener B, Liu H, Lo Y, Sterk R, Petrini J, et al. Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication. DNA Repair (Amst). 2007;6:797-808 pubmed
    ..Interestingly, yku70Delta suppressed BIR in mre11 mutants. BIR is also elevated in rad51 mutants, but yku70Delta did not suppress BIR in a rad51 background...
  7. Davis A, Symington L. The Rad52-Rad59 complex interacts with Rad51 and replication protein A. DNA Repair (Amst). 2003;2:1127-34 pubmed
    ..Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-..
  8. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..Thus, RPA is required to generate ssDNA, and also to protect ssDNA from degradation and inappropriate annealing that could lead to genome rearrangements. ..
  9. Nimonkar A, Dombrowski C, Siino J, Stasiak A, Stasiak A, Kowalczykowski S. Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem. 2012;287:28727-37 pubmed publisher
    ..Dmc1, a meiosis-specific paralog of Rad51, mediates the pairing of homologous chromosomes...
  10. Kelly M, Alver B, Kirkpatrick D. Minisatellite alterations in ZRT1 mutants occur via RAD52-dependent and RAD52-independent mechanisms in quiescent stationary phase yeast cells. DNA Repair (Amst). 2011;10:556-66 pubmed publisher
    ..We propose that the mechanism of ZRT1-mediated minisatellite instability during quiescence is relevant to human cells, and thus, human disease. ..
  11. Pfander B, Moldovan G, Sacher M, Hoege C, Jentsch S. SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase. Nature. 2005;436:428-33 pubmed
    ..SUMO-modified PCNA functionally cooperates with Srs2, a helicase that blocks recombinational repair by disrupting Rad51 nucleoprotein filaments...
  12. Schmidt K, Wu J, Kolodner R. Control of translocations between highly diverged genes by Sgs1, the Saccharomyces cerevisiae homolog of the Bloom's syndrome protein. Mol Cell Biol. 2006;26:5406-20 pubmed
    ..The translocation structures observed suggest involvement of a dicentric intermediate and break-induced replication with multiple cycles of DNA template switching. ..
  13. Pohl T, Nickoloff J. Rad51-independent interchromosomal double-strand break repair by gene conversion requires Rad52 but not Rad55, Rad57, or Dmc1. Mol Cell Biol. 2008;28:897-906 pubmed
    ..In yeast, HR is catalyzed by the Rad51 strand transferase and its "mediators," including the Rad52 single-strand DNA-annealing protein, two ..
  14. Kerrest A, Anand R, Sundararajan R, Bermejo R, Liberi G, Dujon B, et al. SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombination. Nat Struct Mol Biol. 2009;16:159-67 pubmed publisher
    ..Deletion of RAD52 or RAD51 suppresses these phenotypes, suggesting that recombination triggers trinucleotide repeat instability in srs2Delta ..
  15. Chavez A, Agrawal V, Johnson F. Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complex. J Biol Chem. 2011;286:5119-25 pubmed publisher
    ..These data as a whole highlight a role for Smc5/6 and Sgs1 in the resolution of Mph1-dependent HR intermediates. ..
  16. Schmidt K, Kolodner R. Suppression of spontaneous genome rearrangements in yeast DNA helicase mutants. Proc Natl Acad Sci U S A. 2006;103:18196-201 pubmed
    ..Moreover, helicase double mutants accumulate Rad51-dependent Ddc2 foci, indicating the presence of recombination intermediates that are sensed by checkpoints...
  17. Dubrana K, van Attikum H, Hediger F, Gasser S. The processing of double-strand breaks and binding of single-strand-binding proteins RPA and Rad51 modulate the formation of ATR-kinase foci in yeast. J Cell Sci. 2007;120:4209-20 pubmed
    ..Conversely, loss of Rad51 enhanced Mec1 focus formation independently of ssDNA formation, suggesting that Rad51 might compete for the ..
  18. Matsuzaki K, Terasawa M, Iwasaki D, Higashide M, Shinohara M. Cyclin-dependent kinase-dependent phosphorylation of Lif1 and Sae2 controls imprecise nonhomologous end joining accompanied by double-strand break resection. Genes Cells. 2012;17:473-93 pubmed publisher
    ..CDK-dependent modification of the NHEJ pathway might make DSB ends compatible for NHEJ and thus prevent competition between HR and NHEJ in hierarchy on the choice of DSB repair pathways. ..
  19. Schild D. Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or mating-type heterozygosity. Genetics. 1995;140:115-27 pubmed
    ..Because other researchers have shown that the RAD51 and RAD52 proteins interact, RAD51 on a high copy number plasmid was tested and found to suppress the rad52-20 ..
  20. Mazin A, Alexeev A, Kowalczykowski S. A novel function of Rad54 protein. Stabilization of the Rad51 nucleoprotein filament. J Biol Chem. 2003;278:14029-36 pubmed
    Homologous recombination is important for the repair of double-stranded DNA breaks in all organisms. Rad51 and Rad54 proteins are two key components of the homologous recombination machinery in eukaryotes...
  21. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Epistasis analysis suggests that DMC1 and RAD51 function in separate pathways responsible for meiotic recombination...
  22. Bai Y, Davis A, Symington L. A novel allele of RAD52 that causes severe DNA repair and recombination deficiencies only in the absence of RAD51 or RAD59. Genetics. 1999;153:1117-30 pubmed
    ..we have shown that mitotic recombination is dependent on the RAD52 gene, but reduced only fivefold by mutation of RAD51. RAD59, a component of the RAD51-independent pathway, was identified previously by screening for mutations that ..
  23. Hays S, Firmenich A, Massey P, Banerjee R, Berg P. Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA. Mol Cell Biol. 1998;18:4400-6 pubmed
    ..Both of the mutant proteins are capable of self-interaction but are unable to interact with Rad51. The mutant proteins also lack the ability to interact with the large subunit of RPA, Rfa1...
  24. Ho C, Mazon G, Lam A, Symington L. Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast. Mol Cell. 2010;40:988-1000 pubmed publisher
    ....
  25. Lin Y, Chang C, Wong C, Teng S. Recruitment of Rad51 and Rad52 to short telomeres triggers a Mec1-mediated hypersensitivity to double-stranded DNA breaks in senescent budding yeast. PLoS ONE. 2009;4:e8224 pubmed publisher
    ..we also observed that when cells equipped with short telomeres, recruitments of homologous recombination proteins, Rad51 and Rad52, were reduced at an HO-endonuclease-catalyzed double-strand break (DSB), while their associations were ..
  26. Tishkoff D, Filosi N, Gaida G, Kolodner R. A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair. Cell. 1997;88:253-63 pubmed
    ..Mutations in RAD27 cause increased rates of mitotic crossing over and are lethal in combination with mutations in RAD51 and RAD52...
  27. Bergink S, Ammon T, Kern M, Schermelleh L, Leonhardt H, Jentsch S. Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51-Rad52 interaction. Nat Cell Biol. 2013;15:526-32 pubmed publisher
    ..Our data thus suggest that SUMO-targeted Cdc48 restricts the recombinase Rad51 by counterbalancing the activity of Rad52...
  28. Petukhova G, Stratton S, Sung P. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature. 1998;393:91-4 pubmed
    The Saccharomyces cerevisiae RAD51 and RAD54 genes are both required for the occurrence of homologous recombination and for the repair of double-stranded DNA breaks...
  29. Ragu S, Faye G, Iraqui I, Masurel Heneman A, Kolodner R, Huang M. Oxygen metabolism and reactive oxygen species cause chromosomal rearrangements and cell death. Proc Natl Acad Sci U S A. 2007;104:9747-52 pubmed
    ..growth reduced substantially GCR rates of WT and tsa1 mutants and restored the viability of tsa1 rad6, tsa1 rad51, and tsa1 mre11 double mutants...
  30. Chen Y, Choi K, Szakal B, Arenz J, Duan X, Ye H, et al. Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair. Proc Natl Acad Sci U S A. 2009;106:21252-7 pubmed publisher
    ..We suggest that the Smc5/6 complex can counteract/modulate a pro-recombinogenic function of Mph1 or facilitate the resolution of recombination structures generated by Mph1. ..
  31. Busygina V, Sehorn M, Shi I, Tsubouchi H, Roeder G, Sung P. Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev. 2008;22:786-95 pubmed publisher
    Two RecA orthologs, Rad51 and Dmc1, mediate homologous recombination in meiotic cells. During budding yeast meiosis, Hed1 coordinates the actions of Rad51 and Dmc1 by down-regulating Rad51 activity...
  32. Gangloff S, Soustelle C, Fabre F. Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases. Nat Genet. 2000;25:192-4 pubmed
    ..Yeast SRS2 encodes another DNA helicase involved in the maintenance of genome integrity. Our data suggest that some defects observed in BS, WS or RTS are the consequence of unrestrained recombination. ..
  33. Altmannova V, Eckert Boulet N, Arneric M, Kolesar P, Chaloupkova R, Damborsky J, et al. Rad52 SUMOylation affects the efficiency of the DNA repair. Nucleic Acids Res. 2010;38:4708-21 pubmed publisher
    ..Taken together, our results highlight the importance of Rad52 SUMOylation as part of a 'quality control' mechanism regulating the efficiency of recombination and DNA repair. ..
  34. Shah P, Zheng X, Epshtein A, Carey J, Bishop D, Klein H. Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Mol Cell. 2010;39:862-72 pubmed publisher
    ..Here, we show that Rad51 complexes are dissociated by these translocases in mitotic cells...
  35. Sung P. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science. 1994;265:1241-3 pubmed
    The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair...
  36. Kalocsay M, Hiller N, Jentsch S. Chromosome-wide Rad51 spreading and SUMO-H2A.Z-dependent chromosome fixation in response to a persistent DNA double-strand break. Mol Cell. 2009;33:335-43 pubmed publisher
    ..DSB repair involves the sequential recruitment of repair factors to the DSBs, followed by Rad51-mediated homology probing, DNA synthesis, and ligation...
  37. Iraqui I, Faye G, Ragu S, Masurel Heneman A, Kolodner R, Huang M. Human peroxiredoxin PrxI is an orthologue of yeast Tsa1, capable of suppressing genome instability in Saccharomyces cerevisiae. Cancer Res. 2008;68:1055-63 pubmed publisher
    ..a variety of defects including genome instability, the synthetic lethality observed in rad6 Delta tsa1Delta and rad51 Delta tsa1Delta double mutants, and mutagen sensitivity...
  38. Kang L, Symington L. Aberrant double-strand break repair in rad51 mutants of Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:9162-72 pubmed
    A number of studies of Saccharomyces cerevisiae have revealed RAD51-independent recombination events...
  39. Branzei D, Sollier J, Liberi G, Zhao X, Maeda D, Seki M, et al. Ubc9- and mms21-mediated sumoylation counteracts recombinogenic events at damaged replication forks. Cell. 2006;127:509-22 pubmed
    ..ubc9 cells maintain stalled-fork stability but exhibit a Rad51-dependent accumulation of cruciform structures during replication of damaged templates...
  40. Dong Z, Fasullo M. Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes. Nucleic Acids Res. 2003;31:2576-85 pubmed
    ..In comparison with wild type, rates of spontaneous SCE are 10-fold lower in rad51 rad1 but not in either rad51 rad50 or rad51 rad59 double mutants...
  41. Krejci L, Damborsky J, Thomsen B, Duno M, Bendixen C. Molecular dissection of interactions between Rad51 and members of the recombination-repair group. Mol Cell Biol. 2001;21:966-76 pubmed
    ..In the yeast Saccharomyces cerevisiae, recombination requires products of the RAD52 epistasis group. The Rad51 protein associates with the Rad51, Rad52, Rad54, and Rad55 proteins to form a dynamic complex...
  42. Alzu A, Bermejo R, Begnis M, Lucca C, Piccini D, Carotenuto W, et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell. 2012;151:835-46 pubmed publisher
    ....
  43. Gangavarapu V, Santa Maria S, Prakash S, Prakash L. Requirement of replication checkpoint protein kinases Mec1/Rad53 for postreplication repair in yeast. MBio. 2011;2:e00079-11 pubmed publisher
    ..We discuss this important issue and suggest that lesion bypass in Saccharomyces cerevisiae cells occurs in conjunction with the stalled replication forks and not in gaps. ..
  44. Gangavarapu V, Prakash S, Prakash L. Requirement of RAD52 group genes for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:7758-64 pubmed
    ..Here, we examine the contributions of the RAD51, RAD52, and RAD54 genes and of the RAD50 and XRS2 genes to the PRR of UV-damaged DNA...
  45. Niu H, Wan L, Busygina V, Kwon Y, Allen J, Li X, et al. Regulation of meiotic recombination via Mek1-mediated Rad54 phosphorylation. Mol Cell. 2009;36:393-404 pubmed publisher
    ..and the meiosis-specific kinase Mek1, which suppresses engagement of sister chromatids by the mitotic recombinase Rad51. Here, a combination of proteomic, biochemical, and genetic approaches has identified an additional role for ..
  46. Krejci L, Macris M, Li Y, Van Komen S, Villemain J, Ellenberger T, et al. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. J Biol Chem. 2004;279:23193-9 pubmed
    ..ss) DNA-dependent ATPase activity, a DNA helicase activity, and an ability to disassemble the Rad51-ssDNA nucleoprotein filament, which is the key catalytic intermediate in Rad51-mediated recombination reactions...
  47. Wu L, Davies S, Levitt N, Hickson I. Potential role for the BLM helicase in recombinational repair via a conserved interaction with RAD51. J Biol Chem. 2001;276:19375-81 pubmed
    ..In eukaryotes, a central step in homologous recombination is catalyzed by the RAD51 protein...
  48. Jiang H, Xie Y, Houston P, Stemke Hale K, Mortensen U, Rothstein R, et al. Direct association between the yeast Rad51 and Rad54 recombination proteins. J Biol Chem. 1996;271:33181-6 pubmed
    The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae...
  49. Gasior S, Olivares H, Ear U, Hari D, Weichselbaum R, Bishop D. Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis. Proc Natl Acad Sci U S A. 2001;98:8411-8 pubmed
    ..Immuno-double-staining experiments in Saccharomyces cerevisiae suggest that Rad51, the eukaryotic recombinase, can assemble at or near sites containing ssb (replication protein A, RPA) during the ..
  50. Papouli E, Chen S, Davies A, Huttner D, Krejci L, Sung P, et al. Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. Mol Cell. 2005;19:123-33 pubmed
    ..Our findings suggest a mechanism by which SUMO and ubiquitin cooperatively control the choice of pathway for the processing of DNA lesions during replication. ..
  51. Agmon N, Pur S, Liefshitz B, Kupiec M. Analysis of repair mechanism choice during homologous recombination. Nucleic Acids Res. 2009;37:5081-92 pubmed publisher
    ..In addition, we show that increasing the distance between two repeated sequences enhances the dependence on Rad51 for colony formation after DSB repair...
  52. Dupaigne P, Le Breton C, Fabre F, Gangloff S, Le Cam E, Veaute X. The Srs2 helicase activity is stimulated by Rad51 filaments on dsDNA: implications for crossover incidence during mitotic recombination. Mol Cell. 2008;29:243-54 pubmed publisher
    Saccharomyces cerevisiae Srs2 helicase was shown to displace Rad51 in vitro upon translocation on single-stranded DNA...
  53. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..are rescued by MRX-Rad52-Rfc1-dependent recombination via two types of replication restart mechanisms, one being Rad51 dependent and the other Pol32 dependent...
  54. Ira G, Malkova A, Liberi G, Foiani M, Haber J. Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast. Cell. 2003;115:401-11 pubmed
    ..Overexpressing SRS2 nearly eliminates crossovers, whereas overexpression of RAD51 in srs2Delta cells almost completely eliminates the noncrossover recombination pathway...
  55. Henry J, Camahort R, Rice D, Florens L, Swanson S, Washburn M, et al. Mnd1/Hop2 facilitates Dmc1-dependent interhomolog crossover formation in meiosis of budding yeast. Mol Cell Biol. 2006;26:2913-23 pubmed
    ..Recombination in meiosis in Saccharomyces cerevisiae relies on two Escherichia coli recA homologs, Rad51 and Dmc1, as well as the more recently discovered heterodimer Mnd1/Hop2. Meiotic recombination in S...
  56. Fortin G, Symington L. Mutations in yeast Rad51 that partially bypass the requirement for Rad55 and Rad57 in DNA repair by increasing the stability of Rad51-DNA complexes. EMBO J. 2002;21:3160-70 pubmed
    Yeast Rad51 promotes homologous pairing and strand exchange in vitro, but this activity is inefficient in the absence of the accessory proteins, RPA, Rad52, Rad54 and the Rad55-Rad57 heterodimer...
  57. Rattray A, Symington L. Multiple pathways for homologous recombination in Saccharomyces cerevisiae. Genetics. 1995;139:45-56 pubmed
    ..The majority of recombination events are mediated by a RAD51-dependent pathway, where the RAD54, RAD55 and RAD57 genes function downstream of RAD51...
  58. McVey M, Kaeberlein M, Tissenbaum H, Guarente L. The short life span of Saccharomyces cerevisiae sgs1 and srs2 mutants is a composite of normal aging processes and mitotic arrest due to defective recombination. Genetics. 2001;157:1531-42 pubmed
    ..This arrest can be suppressed by mutations in RAD51, RAD52, and RAD57, suggesting that the cell cycle defect in sgs1 srs2 mutants results from inappropriate ..
  59. Symington L. Homologous recombination is required for the viability of rad27 mutants. Nucleic Acids Res. 1998;26:5589-95 pubmed
    ..by crossing a strain containing a null allele of RAD27 to strains containing a mutation in either the RAD1, RAD50, RAD51, RAD52, RAD54, RAD55, RAD57, MRE11, XRS2 or RAD59 gene...
  60. Song B, Sung P. Functional interactions among yeast Rad51 recombinase, Rad52 mediator, and replication protein A in DNA strand exchange. J Biol Chem. 2000;275:15895-904 pubmed
    b>Rad51-catalyzed DNA strand exchange is greatly enhanced by the single-stranded (ss) DNA binding factor RPA if the latter is introduced after Rad51 has already nucleated onto the initiating ssDNA substrate...
  61. Ivanov E, Sugawara N, Fishman Lobell J, Haber J. Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics. 1996;142:693-704 pubmed
    ..We show that RAD51, RAD54, RAD55, and RAD57 genes are not required for SSA irrespective of whether recombination occurred in plasmid ..
  62. Fasullo M, Dong Z, Sun M, Zeng L. Saccharomyces cerevisiae RAD53 (CHK2) but not CHK1 is required for double-strand break-initiated SCE and DNA damage-associated SCE after exposure to X rays and chemical agents. DNA Repair (Amst). 2005;4:1240-51 pubmed
    ..These data indicate that RAD53, not CHK1, is required for DSB-initiated SCE, and DNA damage-associated SCE after exposure to X-ray-mimetic and UV-mimetic chemicals. ..