Genomes and Genes
Gene Symbol: SWR1
Description: chromatin-remodeling protein SWR1
Species: Saccharomyces cerevisiae S288c
- Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone lossHaiying Zhang
Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
Cell 123:219-31. 2005..Specific Htz1 deposition requires the SWR1 complex, which largely colocalizes with Htz1...
- A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatinMichael S Kobor
Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
PLoS Biol 2:E131. 2004..Z in Saccharomyces cerevisiae. This complex was designated SWR1-Com in reference to the Swr1p subunit, a Swi2/Snf2-paralog...
- Stepwise histone replacement by SWR1 requires dual activation with histone H2A.Z and canonical nucleosomeEd Luk
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Cell 143:725-36. 2010..This incorporation is mediated by the conserved SWR1 complex, which replaces histone H2A in canonical nucleosomes with H2A.Z in an ATP-dependent manner...
- Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacementAnand Ranjan
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA Electronic address
Cell 154:1232-45. 2013..Whereas the multisubunit chromatin remodeler SWR1 is known to catalyze ATP-dependent deposition of H2A.Z, the mechanism of SWR1 recruitment to S...
- N terminus of Swr1 binds to histone H2AZ and provides a platform for subunit assembly in the chromatin remodeling complexWei Hua Wu
Laboratory of Biochemistry and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:6200-7. 2009..In Saccharomyces cerevisiae, chromatin deposition of histone H2AZ is mediated by the fourteen-subunit SWR1 complex, which catalyzes ATP-dependent exchange of nucleosomal histone H2A for H2AZ...
- The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPasesHeather Szerlong
Howard Hughes Medical Institute, Department of Oncological Sciences, Huntsman Cancer Institute, 2000 Circle of Hope, University of Utah, Salt Lake City, Utah 84112, USA
Nat Struct Mol Biol 15:469-76. 2008..Individual HSA domains from chromatin remodelers (RSC, yeast SWI-SNF, human SWI-SNF, SWR1 and INO80) or modifiers (NuA4) reconstitute their respective ARP-ARP or ARP-actin modules...
- Distinct roles for SWR1 and INO80 chromatin remodeling complexes at chromosomal double-strand breaksHaico van Attikum
Division of Epigenetics, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
EMBO J 26:4113-25. 2007INO80 and SWR1 are two closely related ATP-dependent chromatin remodeling complexes that share several subunits...
- SAS-mediated acetylation of histone H4 Lys 16 is required for H2A.Z incorporation at subtelomeric regions in Saccharomyces cerevisiaeWei Jong Shia
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
Genes Dev 20:2507-12. 2006..Z incorporation near telomeres. The presence of H4 Lys 16 acetylation and H2A.Z synergistically prevent the ectopic propagation of heterochromatin. Overall, our data suggest a novel antisilencing mechanism near telomeres...
- Interplay between Ino80 and Swr1 chromatin remodeling enzymes regulates cell cycle checkpoint adaptation in response to DNA damageManolis Papamichos-Chronakis
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 20:2437-49. 2006Ino80 and Swr1 are ATP-dependent chromatin remodeling enzymes that have been implicated in DNA repair. Here we show that Ino80 is required for cell cycle checkpoint adaptation in response to a persistent DNA double-strand break (DSB)...
- Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchangeWei Hua Wu
Laboratory of Molecular Cell Biology, Center for Cancer Research, National Cancer Institute, US National Institutes of Health, Building 37, Room 6068, Bethesda, Maryland 20892 4255, USA
Nat Struct Mol Biol 12:1064-71. 2005..In Saccharomyces cerevisiae, deposition of histone H2AZ is mediated by the multiprotein SWR1 complex, which catalyzes ATP-dependent exchange of nucleosomal histone H2A for H2AZ...
- ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complexGaku Mizuguchi
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 6068, Bethesda, MD 20892 4255, USA
Science 303:343-8. 2004..How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. We have found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger ..
- Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-CShinya Watanabe
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, Maasachusetts 01605, USA
Nat Commun 6:7108. 2015..In contrast, the Ies6/Arp5 module is essential for INO80-C remodelling, and furthermore this module controls conformational changes that may couple nucleosome binding to remodelling. ..
- Deposition of histone H2A.Z by the SWR-C remodeling enzyme prevents genome instabilityChristopher Van
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, United States
DNA Repair (Amst) 25:9-14. 2015..Exo1 function, because single base mutator effects are seen in both Exo1-proficient and Exo1-deficient pol3-L612M swr1Δ strains. The data are consistent with the possibility that incorporation of the H2A...
- Maintenance of heterochromatin boundary and nucleosome composition at promoters by the Asf1 histone chaperone and SWR1-C chromatin remodeler in Saccharomyces cerevisiaePhoebe Y T Lu
Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia, Vancouver, BC, Canada V5Z 4H4
Genetics 197:133-45. 2014..Building on a previously reported link between the histone chaperone Asf1 and the Yaf9 subunit of the SWR1-C chromatin remodeler, we found that ASF1 broadly interacted with genes encoding for SWR1-C subunits...
- Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiaeMoriah Eustice
Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0347
Genetics 196:1029-39. 2014..Our analysis thus uncovers previously unsuspected functions for both Gas1 and Sas3 in DNA damage response and cell cycle regulation. ..
- The catalytic subunit of the SWR1 remodeler is a histone chaperone for the H2A.Z-H2B dimerJingjun Hong
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Mol Cell 53:498-505. 2014..The multisubunit nucleosome-remodeling enzyme complex SWR1, conserved from yeast to mammals, catalyzes the ATP-dependent replacement of histone H2A in canonical nucleosomes ..
- H2A histone-fold and DNA elements in nucleosome activate SWR1-mediated H2A.Z replacement in budding yeastAnand Ranjan
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
elife 4:e06845. 2015..The chromatin remodeler SWR1 mediates site-specific incorporation of H2A...
- A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzymeShinya Watanabe
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 340:195-9. 2013..Z from nucleosomes. Our study identifies a histone modification that regulates a chromatin remodeling reaction and provides insights into how histone variants and nucleosome turnover can be controlled by chromatin regulators...
- Bdf1 Bromodomains are Essential for Meiosis and the Expression of Meiotic-Specific GenesEncar García-Oliver
U1038, Université Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble BIG, Grenoble, France
PLoS Genet 13:e1006541. 2017..Taken together, our results unveil a new role for Bdf1 -working independently from its predominant protein partners Bdf2 and the SWR1 complex-as a regulator of meiosis-specific genes.
- Maintenance of low histone ubiquitylation by Ubp10 correlates with telomere-proximal Sir2 association and gene silencingN C Tolga Emre
Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, PA 19024, USA
Mol Cell 17:585-94. 2005..Our results suggest that these H2B-deubiquitylating enzymes have distinct genomic functions...
- Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1Vu Q Nguyen
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Cell 154:1220-31. 2013The ATP-dependent chromatin-remodeling complex SWR1 exchanges a variant histone H2A.Z/H2B dimer for a canonical H2A/H2B dimer at nucleosomes flanking histone-depleted regions, such as promoters. This localization of H2A...
- RNA polymerase II carboxyl-terminal domain phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36Stephen M Fuchs
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
J Biol Chem 287:3249-56. 2012..Collectively, these results provide molecular insight into the regulation of Set2 protein levels that influence H3K36 methylation states...
- Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break endsDiego Bonetti
Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milano, Italy
EMBO J 32:275-89. 2013..Finally, inactivation of either Tbf1 or Vid22 impairs nucleosome eviction around the DSB, suggesting that these proteins promote efficient repair of the break by influencing chromatin identity in its surroundings...
- ATP-dependent chromatin remodeling and histone acetyltransferases in 5-FU cytotoxicity in Saccharomyces cerevisiaeR Matuo
Centro de Biotecnologia, Departamento de Biofisica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
Genet Mol Res 12:1440-56. 2013..Combined deficiencies in HAT with defects in HR and PRR did not potentiate 5-FU cytotoxicity, possibly because they function in a common pathway...
- Histone variant H2A.Z functions in sister chromatid cohesion in Saccharomyces cerevisiaeUpasna Sharma
Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut, USA
Mol Cell Biol 33:3473-81. 2013..H2A.Z is deposited into chromatin by the SWR1 complex and is subject to acetylation of its four N-terminal tail lysine residues by the NuA4 and SAGA histone ..
- The functional role of SUMO E3 ligase Mms21p in the maintenance of subtelomeric silencing in budding yeastYakun Wan
The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China
Biochem Biophys Res Commun 438:746-52. 2013..Taken together, our findings suggest an important role of Mms21p; it contributes to subtelomeric silencing during the formation of a heterochromatin boundary. ..
- Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongationSandra Holbein
Institute of Molecular Biology, University of Zurich, CH 8057 Zurich, Switzerland
RNA 15:837-49. 2009..Finally, chemical-genetic profiling revealed genome-wide pathways linked to cordycepin activity and identified novel genes involved in poly(A) homeostasis...
- The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeresHaiying Zhang
Department of Oncological Sciences, School of Medicine, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112, USA
Mol Cell Biol 24:9424-36. 2004..Yaf9 is also associated with the SWR1 complex, which deposits the histone H2A variant Htz1...
- SWR1 complex poises heterochromatin boundaries for antisilencing activity propagationBo O Zhou
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, 320 Yue Yang Road, Shanghai 200031, China
Mol Cell Biol 30:2391-400. 2010..In this study, we show that the chromatin-remodeling complex SWR1 (SWR1-C) uses a distinct strategy to regulate heterochromatin spreading...
- Isw1 functions in parallel with the NuA4 and Swr1 complexes in stress-induced gene repressionKimberly C Lindstrom
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell Biol 26:6117-29. 2006..We identified a three-way genetic interaction among Isw1, the NuA4 histone acetyltransferase complex, and the Swr1 histone replacement complex...
- Chz1, a nuclear chaperone for histone H2AZEd Luk
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 25:357-68. 2007..The incorporation of H2AZ into chromatin is dependent on the SWR1 complex, which catalyses the replacement of conventional histone H2A with H2AZ...
- Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiaeMatthew Jessulat
Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont, Canada K1S 5B6
Arch Biochem Biophys 469:157-64. 2008..We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination...
- Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variantsAndréanne Auger
Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, 9 McMahon St, Quebec City, Quebec G1R 2J6, Canada
Mol Cell Biol 28:2257-70. 2008..This domain is highly related to the yeast Swr1 protein, which is responsible for the incorporation of histone variant H2AZ in chromatin...
- Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathwaysStephane Hausmann
Department of Microbiology and Molecular Medicine, University of Geneva, CH1211 Geneva, Switzerland
J Biol Chem 283:31706-18. 2008..processing and decay (Pat1, Lsm1, and Trf4) and regulation of polymerase II transcription (Rpn4, Spt3, Srb2, Soh1, Swr1, and Htz1). We find that the C-terminal domain of human Tgs1 can function in lieu of the yeast protein in vivo...
- Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genomeR Thomas Koerber
Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
Mol Cell 35:889-902. 2009..we examine on a genomic scale several protein-nucleosome interactions, including those that (1) bind histones (Bdf1/SWR1 and Srm1), (2) bind specific DNA sequences (Rap1 and Reb1), and (3) potentially collide with nucleosomes during ..
- Histone variant H2A.Z and RNA polymerase II transcription elongationMaria Soledad Santisteban
Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
Mol Cell Biol 31:1848-60. 2011....
- Asf1-like structure of the conserved Yaf9 YEATS domain and role in H2A.Z deposition and acetylationAlice Y Wang
Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada V5Z 4H4
Proc Natl Acad Sci U S A 106:21573-8. 2009..of both the essential histone acetyltransferase complex NuA4 and the ATP-dependent chromatin remodeling complex SWR1-C, which deposits histone variant H2A.Z into euchromatin...
- The SWR1 histone replacement complex causes genetic instability and genome-wide transcription misregulation in the absence of H2A.ZMacarena Morillo-Huesca
Department of Molecular Biology, CABIMER CSIC, Seville, Spain
PLoS ONE 5:e12143. 2010The SWR1 complex replaces the canonical histone H2A with the variant H2A.Z (Htz1 in yeast) at specific chromatin regions...
- Roles for H2A.Z and its acetylation in GAL1 transcription and gene induction, but not GAL1-transcriptional memoryJeffrey E Halley
Department of Molecular and Cell Biology, and California Institute for Quantitative Biosciences, University of California Berkeley, Berkeley, California, United States
PLoS Biol 8:e1000401. 2010..Z, the SWR1 complex, which deposits H2A...
- Cks1 activates transcription by binding to the ubiquitylated proteasomeRoman Holic
Eukaryotic Chromatin Dynamics Group, MRC Clinical Sciences Centre, Imperial College Hammersmith Campus, London, United Kingdom
Mol Cell Biol 30:3894-901. 2010....
- Actin-related protein Arp6 influences H2A.Z-dependent and -independent gene expression and links ribosomal protein genes to nuclear poresTakahito Yoshida
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
PLoS Genet 6:e1000910. 2010..examined the role of the budding yeast actin-related protein Arp6 in gene expression, both as a component of the SWR1 complex (SWR-C) and in its absence...
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 2007..To understand the rules and mechanisms for these assembly patterns, we will engineer mutations in specific cis- and trans-acting factors to test alternative models of Htz1 recruitment. ..
- Epigenetic Regulation of Gene Expression During Early Mouse EmbryogenesisM Smith; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- The Role of MYST histone acetyltransferases in genome stabilityM Smith; Fiscal Year: 2008..These experiments will greatly expand our understanding of Hbo1 function in regulating DNA replication and cell proliferation. ..