DCL1

Summary

Gene Symbol: DCL1
Description: dicer-like 1
Alias: ABNORMAL SUSPENSOR 1, ASU1, ATDCL1, CAF, CARPEL FACTORY, DICER-LIKE 1, EMB60, EMB76, EMBRYO DEFECTIVE 60, EMBRYO DEFECTIVE 76, SHORT INTEGUMENTS 1, SIN1, SUS1, SUSPENSOR 1, T25K16.4, T25K16_4, dicer-like 1
Species: thale cress

Top Publications

  1. pmc MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis
    Michael Ronemus
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Plant Cell 18:1559-74. 2006
  2. pmc DICER-LIKE 1 and DICER-LIKE 3 redundantly act to promote flowering via repression of FLOWERING LOCUS C in Arabidopsis thaliana
    Robert J Schmitz
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 176:1359-62. 2007
  3. pmc Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1
    Feng Qu
    School of Biological Sciences, University of Nebraska, 206 Morrison Center, Lincoln, NE 68583 0900, USA
    Proc Natl Acad Sci U S A 105:14732-7. 2008
  4. pmc Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants
    Yuda Fang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Curr Biol 17:818-23. 2007
  5. pmc The helicase and RNaseIIIa domains of Arabidopsis Dicer-Like1 modulate catalytic parameters during microRNA biogenesis
    Chenggang Liu
    Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Plant Physiol 159:748-58. 2012
  6. pmc MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis
    Michael D Nodine
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genes Dev 24:2678-92. 2010
  7. pmc Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
    Sascha Laubinger
    Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 107:17466-73. 2010
  8. pmc NOT2 proteins promote polymerase II-dependent transcription and interact with multiple MicroRNA biogenesis factors in Arabidopsis
    Lulu Wang
    State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant Cell 25:715-27. 2013
  9. pmc Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction
    Haina Qin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    RNA 16:474-81. 2010
  10. pmc Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies
    Qi Liu
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Plant Physiol 163:108-17. 2013

Research Grants

  1. 2007 Epigenetics Gordon Research Conference
    Steven Jacobsen; Fiscal Year: 2007
  2. Genetics of DNA Methylation Patterning of Arabidopsis
    Steven Jacobsen; Fiscal Year: 2008

Scientific Experts

  • Liang Wu
  • Steven Jacobsen
  • Xuemei Chen
  • Yuda Fang
  • Yin Liu
  • Qi Liu
  • Shuxin Zhang
  • Bin Yu
  • Zhenfei Sun
  • Irina P Suarez
  • Paula Burdisso
  • Satoru Machida
  • Matthew R Willmann
  • Yuichiro Watanabe
  • Simona Baranauskė
  • Xiaofeng Fang
  • Xi Yang
  • William F Seefried
  • Leilei Shi
  • Rodolfo M Rasia
  • Qingqing Yan
  • Yuji Iwata
  • Lulu Wang
  • Hongliang Zhu
  • Michael J Axtell
  • Nina V Fedoroff
  • Pablo D Jenik
  • Chenggang Liu
  • Y Adam Yuan
  • Haina Qin
  • Michael D Nodine
  • Erika Lackey
  • Sascha Laubinger
  • Yuko Tagami
  • Marie Noëlle Pouch-Pélissier
  • Feng Qu
  • Liang Song
  • Surekha Katiyar-Agarwal
  • Herve Vaucheret
  • Patrice Dunoyer
  • Yoichiro Fujioka
  • Robert J Schmitz
  • Todd Blevins
  • Nicolas Bouche
  • Michael Ronemus
  • Yukio Kurihara
  • Ian R Henderson
  • Akihiro Hiraguri
  • Sizolwenkosi Mlotshwa
  • Edwards Allen
  • Yijun Qi
  • Zulong Chen
  • Giedrius Vilkaitis
  • Andreas Finke
  • Xiuli Lu
  • Tongtong Guo
  • Ceslovas Venclovas
  • Alexandra Plotnikova
  • Saulius Klimasauskas
  • Yupeng Shi
  • Matthieu P M H Benoit
  • Milda Mickutė
  • Jerome Boisbouvier
  • Feijie Wu
  • Peng Zhang
  • Wenqing Ren
  • Yuhui Liu
  • Qiuxia Zhao
  • Yuke He
  • Rachel L Clausen
  • Wonkeun Park
  • Zhaohu Li
  • Liusheng Duan
  • Samir M Hamdan
  • Ying Tao Zhao
  • Xiaofeng Cao
  • Xianwei Song
  • Chunxiao Ge
  • Fang Hong
  • Amber Lu
  • Claudia Castillo-González
  • Meng Xie
  • Xiu Jie Wang
  • Yuyi Zhou
  • Ying Huang
  • Chengcai Chu
  • Masateru Takahashi
  • Lianfeng Gu
  • Shouyun Cao
  • Xin Li

Detail Information

Publications44

  1. pmc MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis
    Michael Ronemus
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Plant Cell 18:1559-74. 2006
    ..wild-type plants but not in ago1, the RNA-dependent RNA polymerase mutants rdr2 and rdr6, or the Dicer-like mutants dcl1 and dcl3...
  2. pmc DICER-LIKE 1 and DICER-LIKE 3 redundantly act to promote flowering via repression of FLOWERING LOCUS C in Arabidopsis thaliana
    Robert J Schmitz
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 176:1359-62. 2007
    ..This delayed-flowering phenotype is similar to that of autonomous-pathway mutants, and the flowering delay can be overcome by vernalization...
  3. pmc Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1
    Feng Qu
    School of Biological Sciences, University of Nebraska, 206 Morrison Center, Lincoln, NE 68583 0900, USA
    Proc Natl Acad Sci U S A 105:14732-7. 2008
    ..Surprisingly, we found that DCL1 represses antiviral RNA silencing through negatively regulating the expression of DCL4 and DCL3...
  4. pmc Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants
    Yuda Fang
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Curr Biol 17:818-23. 2007
    ..the second step cytoplasmic, whereas in plant cells, both steps occur in the nucleus via the enzyme Dicer-like1 (DCL1) and other proteins including the zinc-finger-domain protein Serrate (SE) and a double-stranded RNA (dsRNA) binding-..
  5. pmc The helicase and RNaseIIIa domains of Arabidopsis Dicer-Like1 modulate catalytic parameters during microRNA biogenesis
    Chenggang Liu
    Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Plant Physiol 159:748-58. 2012
    Dicer-Like1 (DCL1), an RNaseIII endonuclease, and Hyponastic Leaves1 (HYL1), a double-stranded RNA-binding protein, are core components of the plant microRNA (miRNA) biogenesis machinery...
  6. pmc MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis
    Michael D Nodine
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genes Dev 24:2678-92. 2010
    Arabidopsis embryos lacking DICER-LIKE1 (DCL1), which is required for microRNA (miRNA) biogenesis, arrest early in development...
  7. pmc Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
    Sascha Laubinger
    Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 107:17466-73. 2010
    ..Our analysis revealed a surprising number of common targets in dcl1 and dcl2 dcl3 dcl4 triple mutants...
  8. pmc NOT2 proteins promote polymerase II-dependent transcription and interact with multiple MicroRNA biogenesis factors in Arabidopsis
    Lulu Wang
    State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Plant Cell 25:715-27. 2013
    ..NOT2 was identified by its interaction with the Piwi/Ago/Zwille domain of DICER-LIKE1 (DCL1), an interaction that is conserved between rice (Oryza sativa) and Arabidopsis thaliana...
  9. pmc Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction
    Haina Qin
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    RNA 16:474-81. 2010
    ..binds to double-stranded RNA-binding domain 1 (dsRBD1) of Arabidopsis DRB4, whereas the DUF283 domain of DCL1 specifically binds to dsRBD2 of Arabidopsis HYL1...
  10. pmc Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies
    Qi Liu
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Plant Physiol 163:108-17. 2013
    ..miRNAs are processed from primary miRNAs (pri-miRNAs), mainly by the ribonuclease III-like enzyme DICER-LIKE1 (DCL1) and its specific partner, HYPONASTIC LEAVES1 (HYL1), a double-strand RNA-binding protein, both of which contain ..
  11. pmc Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1
    Hongliang Zhu
    1 Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas, USA 2 Institute of Plant Genomics and Biotechnology, Texas A and M University, College Station, Texas, USA 3 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    Nat Struct Mol Biol 20:1106-15. 2013
    ..Unlike canonical processing of pri-miRNAs, terminal loop-branched pri-miRNAs can be processed by Dicer-like 1 (DCL1) complexes bidirectionally from base to loop and from loop to base, resulting in productive and abortive processing ..
  12. pmc The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis
    Bin Yu
    Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 105:10073-8. 2008
    ..Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs...
  13. pmc CDC5, a DNA binding protein, positively regulates posttranscriptional processing and/or transcription of primary microRNA transcripts
    Shuxin Zhang
    Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska Lincoln, Lincoln, NE 68588
    Proc Natl Acad Sci U S A 110:17588-93. 2013
    ..These results suggest that CDC5 may have dual roles in miRNA biogenesis: functioning as a positive transcription factor of MIR and/or acting as a component of the DICER-LIKE1 complex to enhance primary miRNA processing. ..
  14. ncbi Location of a possible miRNA processing site in SmD3/SmB nuclear bodies in Arabidopsis
    Yoichiro Fujioka
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3 8 1, Meguro, Tokyo, Japan
    Plant Cell Physiol 48:1243-53. 2007
    ..In plants, it has been reported that Dicer-like 1 (DCL1), HYPONASTIC LEAVES1 (HYL1) and SERRATE (SE) are involved in the production of miRNAs...
  15. pmc An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs
    Nicolas Bouche
    Laboratoire de Biologie Cellulaire, Institut Jean Pierre Bourgin, INRA, Versailles, France
    EMBO J 25:3347-56. 2006
    ..Analyses of single, double, triple and quadruple dcl mutants exposed DCL1 as a sophisticated enzyme capable of producing both microRNAs (miRNAs) and siRNAs, unlike the three other DCLs, ..
  16. ncbi Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning
    Ian R Henderson
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California 90095, USA
    Nat Genet 38:721-5. 2006
    ..Here, we study the role of the four Arabidopsis thaliana DICER-LIKE genes (DCL1-DCL4) in these processes...
  17. pmc The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis
    Yukio Kurihara
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
    RNA 12:206-12. 2006
    ..Here we examine the relationship between the Arabidopsis microRNA- (miRNA) producing enzyme DCL1 and the dsRNA binding protein HYL1...
  18. pmc MicroRNA biogenesis and function in plants
    Xuemei Chen
    Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    FEBS Lett 579:5923-31. 2005
    ..A large category of miRNA targets consists of genes encoding transcription factors that play important roles in patterning the plant form...
  19. ncbi Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana
    Akihiro Hiraguri
    Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Saiwaicho 3 5 8, Fuchu, Tokyo 183 8509, Japan
    Plant Mol Biol 57:173-88. 2005
    ..properties of nine such gene products, each containing one or two dsRBMs: four Arabidopsis Dicer-like proteins (DCL1-4), Arabidopsis HYL1 and four of its homologs (DRB2, DRB4, DRB5 and OsDRB1)...
  20. pmc CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana
    Wonkeun Park
    Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
    Curr Biol 12:1484-95. 2002
    ..In Arabidopsis thaliana, mutations in CARPEL FACTORY (CAF), a Dicer homolog, and those in a novel gene, HEN1, result in similar, multifaceted developmental defects,..
  21. pmc Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body
    Liang Song
    Biology Department and Huck Institute of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 104:5437-42. 2007
    ..work we investigated the role of the Arabidopsis nuclear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pri-miR171a)...
  22. pmc PRL1, an RNA-binding protein, positively regulates the accumulation of miRNAs and siRNAs in Arabidopsis
    Shuxin Zhang
    Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
    PLoS Genet 10:e1004841. 2014
    ..Furthermore, PRL1 interacts with the pri-miRNA processor, DCL1, and the dsRNA processors (DCL3 and DCL4)...
  23. pmc The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs
    Zhenfei Sun
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    PLoS Genet 11:e1005598. 2015
    ..primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1)...
  24. pmc Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA
    Xi Yang
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Nucleic Acids Res 42:12224-36. 2014
    ..HYPONASTIC LEAVES1 (HYL1) is a double-stranded RNA-binding protein that forms a complex with DICER-LIKE1 (DCL1) and SERRATE (SE) to process primary miRNA (pri-miRNA) into mature miRNA...
  25. pmc Global Regulation of Embryonic Patterning in Arabidopsis by MicroRNAs
    William F Seefried
    Department of Biology, Franklin and Marshall College, Lancaster, Pennsylvania 17604 3003 W F S, R L C, P D J andDepartment of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 M R W
    Plant Physiol 165:670-687. 2014
    ..extend those analyses by characterizing embryos mutant for a strong hypomorphic allele of DICER-LIKE1 (dcl1-15)...
  26. pmc Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model
    Yin Liu
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    Mol Biol Cell 25:1366-73. 2014
    ....
  27. pmc Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins
    Simona Baranauskė
    Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, Vilnius LT 02241, Lithuania
    Nucleic Acids Res 43:2802-12. 2015
    ..Furthermore, we find that HEN1 forms a complex with DICER-LIKE 1 (DCL1) ribonuclease, another key protein involved in miRNA biogenesis machinery...
  28. doi CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis
    Xiaofeng Fang
    Graduate Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Mol Plant 8:1227-36. 2015
    ..There are three major classes of sRNAs in Arabidopsis: DCL1-dependent microRNA (miRNA), DCL3-dependent heterochromatic siRNA (hc-siRNA), and DCL4-dependent trans-acting siRNA (..
  29. pmc Induced folding in RNA recognition by Arabidopsis thaliana DCL1
    Irina P Suarez
    Instituto de Biología Molecular y Celular de Rosario 27 de Febrero 210 bis, predio CCT, 2000 Rosario, Argentina Área Biofísica, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario Suipacha 531, 2000 Rosario, Argentina
    Nucleic Acids Res 43:6607-19. 2015
    b>DCL1 is the ribonuclease that carries out miRNA biogenesis in plants. The enzyme has two tandem double stranded RNA binding domains (dsRBDs) in its C-terminus...
  30. pmc MicroRNAs regulate the timing of embryo maturation in Arabidopsis
    Matthew R Willmann
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Plant Physiol 155:1871-84. 2011
    ..Our data point to a common negative regulatory module of maturation during early embryogenesis and seedling development...
  31. pmc Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing
    Yuji Iwata
    Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955 6900, Kingdom of Saudi Arabia and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal 23955 6900, Kingdom of Saudi Arabia
    Nucleic Acids Res 41:9129-40. 2013
    ..and precise microRNA (miRNA) biogenesis in Arabidopsis is mediated by the RNaseIII-family enzyme DICER-LIKE 1 (DCL1), double-stranded RNA-binding protein HYPONASTIC LEAVES 1 and the zinc-finger (ZnF) domain-containing protein ..
  32. pmc Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation
    Liang Wu
    National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    Plant Physiol 160:990-9. 2012
    ..More important, we found that DCL1 is the only DCL protein that is required for TAS3 loci DNA methylation, and all four DCLs exert combinatory ..
  33. doi Second double-stranded RNA binding domain of dicer-like ribonuclease 1: structural and biochemical characterization
    Paula Burdisso
    Instituto de Biologia Molecular y Celular de Rosario IBR CONICET, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Santa Fe, Argentina
    Biochemistry 51:10159-66. 2012
    ..Here we present a structural characterization of the second double-stranded RNA binding domain (dsRBD) of DCL1, which is presumed to participate in pri-micro-RNA recognition and subcellular localization of this protein...
  34. doi Crystal structure of Arabidopsis thaliana Dawdle forkhead-associated domain reveals a conserved phospho-threonine recognition cleft for dicer-like 1 binding
    Satoru Machida
    Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    Mol Plant 6:1290-300. 2013
    ..experiments performed in Nicotiana benthamiana show that the DDL FHA domain co-immuno-precipitates with DCL1 fragments containing the predicted pThr+3(Ile/Val/Leu/Asp) motif...
  35. ncbi SHORT INTEGUMENT (SIN1), a gene required for ovule development in Arabidopsis, also controls flowering time
    A Ray
    Department of Biology, University of Rochester, NY 14627, USA
    Development 122:2631-8. 1996
    The short integument (sin1) mutation causes a female-specific infertility, and a defect in the control of time to flowering in Arabidopsis...
  36. ncbi Disruption of an RNA helicase/RNAse III gene in Arabidopsis causes unregulated cell division in floral meristems
    S E Jacobsen
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Development 126:5231-43. 1999
    ..A new recessive mutant, carpel factory (caf), converts the floral meristems to an indeterminate state...
  37. pmc Ectopic DICER-LIKE1 expression in P1/HC-Pro Arabidopsis rescues phenotypic anomalies but not defects in microRNA and silencing pathways
    Sizolwenkosi Mlotshwa
    Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA
    Plant Cell 17:2873-85. 2005
    ..Here, we investigate the effects of overexpressing DCL1, one of four Dicers in Arabidopsis thaliana, on P1/HC-Pro-induced defects in development and small RNA metabolism...
  38. pmc Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing
    Todd Blevins
    Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056 Basel, Switzerland
    Nucleic Acids Res 34:6233-46. 2006
    ..interfering RNAs (siRNAs) of specific size classes (21, 22 and 24 nt) are produced by all four DCLs, including DCL1, known to process microRNA precursors. Specifically, DCL1 generates 21 nt siRNAs from the CaMV leader region...
  39. ncbi Intra- and intercellular RNA interference in Arabidopsis thaliana requires components of the microRNA and heterochromatic silencing pathways
    Patrice Dunoyer
    Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche Scientifique CNRS UPR2357, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Nat Genet 39:848-56. 2007
    ..Finally, we show that the genetics of RNAi is distinct from that of antiviral silencing and propose that this experimental silencing pathway has a direct endogenous plant counterpart...
  40. pmc A novel class of bacteria-induced small RNAs in Arabidopsis
    Surekha Katiyar-Agarwal
    Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California at Riverside, Riverside, California 92521, USA
    Genes Dev 21:3123-34. 2007
    ..piRNAs/rasiRNAs, which is dicer independent and requires PIWI subfamily proteins, generation of AtlsiRNA-1 requires DCL1, DCL4, and the ARGONAUTE subfamily protein AGO7. It also depends on HYL1, HEN1, HST1, RDR6, and Pol IV...
  41. pmc SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex
    Marie Noëlle Pouch-Pélissier
    Universite de Perpignan Via Domitia, CNRS UMR5096 LGDP, Perpignan, France
    PLoS Genet 4:e1000096. 2008
    ..interacts with HYL1 (DRB1), a double-stranded RNA-binding protein (dsRBP) that associates with the Dicer homologue DCL1 to produce microRNAs...
  42. pmc A dominant mutation in DCL1 suppresses the hyl1 mutant phenotype by promoting the processing of miRNA
    Yuko Tagami
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo 153 8902, Japan
    RNA 15:450-8. 2009
    MicroRNAs (miRNAs) are sequence-specific negative regulators of gene expression generated by DICER-LIKE1 (DCL1) with the assistance of a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1), in Arabidopsis...
  43. pmc RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
    Erika Lackey
    Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, Austin, TX 78712, USA
    New Phytol 186:207-15. 2010
    ..in Arabidopsis using RNA interference (RNAi) that downregulates the expression of miRNA biogenesis genes, including DCL1 and AGO1...
  44. ncbi microRNA-directed phasing during trans-acting siRNA biogenesis in plants
    Edwards Allen
    Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon 97331, USA
    Cell 121:207-21. 2005
    ....

Research Grants2

  1. 2007 Epigenetics Gordon Research Conference
    Steven Jacobsen; Fiscal Year: 2007
    ..Perhaps equally importantly, it will launch the careers of new scientists in this frontier area. [unreadable] [unreadable] [unreadable]..
  2. Genetics of DNA Methylation Patterning of Arabidopsis
    Steven Jacobsen; Fiscal Year: 2008
    ....