VTC2

Summary

Gene Symbol: VTC2
Description: GDP-L-galactose phosphorylase 1
Alias: F10M23.190, F10M23_190, vitamin c defective 2
Species: thale cress

Top Publications

  1. doi Transient expression analysis revealed the importance of VTC2 expression level in light/dark regulation of ascorbate biosynthesis in Arabidopsis
    Kazuya Yoshimura
    a Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University, Kasugai, Japan
    Biosci Biotechnol Biochem 78:60-6. 2014
  2. ncbi Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana
    Yongshun Gao
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
    Biosci Biotechnol Biochem 75:1783-8. 2011
  3. doi Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity
    Christopher J Botanga
    Department of Plant Biology, University of Minnesota, St Paul, USA
    Mol Plant Microbe Interact 25:1628-38. 2012
  4. pmc Ascorbate deficiency can limit violaxanthin de-epoxidase activity in vivo
    Patricia Muller-Moule
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    Plant Physiol 128:970-7. 2002
  5. pmc Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance
    Jane Larkindale
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Plant Physiol 138:882-97. 2005
  6. pmc Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis
    Valeria Pavet
    Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Cordoba 5000, Argentina
    Plant Physiol 139:1291-303. 2005
  7. ncbi Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis
    Yuko Sasaki-Sekimoto
    Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Yokohama, Kanagawa, 226 8501, Japan
    Plant J 44:653-68. 2005
  8. ncbi Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana
    E Olmos
    Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    J Exp Bot 57:1645-55. 2006
  9. pmc Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants
    Carole L Linster
    Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 282:18879-85. 2007
  10. pmc The missing step of the L-galactose pathway of ascorbate biosynthesis in plants, an L-galactose guanyltransferase, increases leaf ascorbate content
    William A Laing
    Horticultural and Food Research Institute of New Zealand, PB 92160, Auckland 1142, New Zealand
    Proc Natl Acad Sci U S A 104:9534-9. 2007

Scientific Experts

  • Patricia Muller-Moule
  • William A Laing
  • Yuko Sasaki Sekimoto
  • Carole L Linster
  • Takahiro Ishikawa
  • Kazuya Yoshimura
  • Takanori Maruta
  • Shigeru Shigeoka
  • Christopher J Botanga
  • Yongshun Gao
  • Nicholas Smirnoff
  • Nicole K Clay
  • Yasunobu Ogura
  • Charles Brenner
  • Lital N Adler
  • Steven G Clarke
  • Kathryn C Christensen
  • John Dowdle
  • E Olmos
  • Valeria Pavet
  • Yuko Sasaki-Sekimoto
  • Jane Larkindale
  • Yuki Shiomi
  • Tomono Nakane
  • Seina Kume
  • Jane Glazebrook
  • Daniel R Gallie
  • Gerit Bethke
  • Oliver Fiehn
  • Zhong Chen
  • Adebanjo Ayobamidele Badejo
  • Mike Page
  • Hitoshi Shibata
  • Yoshihiro Sawa
  • Frederick M Ausubel
  • Carine Denoux
  • Georg Jander
  • Adewale M Adio
  • Kristofor Webb
  • Akihiro Komatsu
  • Kazunori Zikihara
  • Tomohiro Kiyosue
  • Masamitsu Wada
  • Tokihiko Nanjo
  • Satoru Tokutomi
  • Susanne Rolinski
  • Tara A Gomez
  • Stephan Gatzek
  • Brian D Young
  • Tk Pellny
  • Ch Foyer
  • S Kumar
  • G Kiddle
  • Mitsuko Aono
  • Fuminori Matsumoto
  • Christine H Foyer
  • Takeshi Obayashi
  • Masaaki Noji
  • Hideyuki Suzuki
  • Marc R Knight
  • Hiroyuki Ohta
  • Jennifer D Hall
  • Shaheen Mowla
  • Elizabeth Vierling
  • Ken ichiro Takamiya
  • Sanjay Kumar
  • Kazuki Saito
  • Enrique Olmos
  • Guy Kiddle
  • John Antoniw
  • Maria E Alvarez
  • Tatsuru Masuda
  • Daisuke Shibata
  • Nozomi Taki
  • Masami Yokota Hirai
  • Nozomu Sakurai

Detail Information

Publications15

  1. doi Transient expression analysis revealed the importance of VTC2 expression level in light/dark regulation of ascorbate biosynthesis in Arabidopsis
    Kazuya Yoshimura
    a Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University, Kasugai, Japan
    Biosci Biotechnol Biochem 78:60-6. 2014
    ..phosphatase (GPP/VTC4), but AsA levels in the plants transiently expressing GDP-l-Gal phosphorylase (GGP/VTC2) were 2.5-fold higher than those in control plants 7 d after ES treatment...
  2. ncbi Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana
    Yongshun Gao
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
    Biosci Biotechnol Biochem 75:1783-8. 2011
    Arabidopsis thaliana contains two GDP-L-galactose phosphorylase genes, VTC2 and VTC5, which are critical for ascorbate (AsA) biosynthesis. We investigated the expression levels of both VTC2 and VTC5 genes in wild-type A...
  3. doi Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity
    Christopher J Botanga
    Department of Plant Biology, University of Minnesota, St Paul, USA
    Mol Plant Microbe Interact 25:1628-38. 2012
    ..GABA promoted, and ascorbate strongly inhibited, fungal growth in culture. Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to A. brassicicola...
  4. pmc Ascorbate deficiency can limit violaxanthin de-epoxidase activity in vivo
    Patricia Muller-Moule
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    Plant Physiol 128:970-7. 2002
    ..ascorbate levels on NPQ in vivo, we measured the induction of NPQ in a vitamin C-deficient mutant of Arabidopsis, vtc2-2...
  5. pmc Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance
    Jane Larkindale
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Plant Physiol 138:882-97. 2005
    ..Ethylene signaling mutants (ein2 and etr1) and reactive oxygen metabolism mutants (vtc1, vtc2, npq1, and cad2) were more defective in basal than acquired thermotolerance, especially under high light...
  6. pmc Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis
    Valeria Pavet
    Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Cordoba 5000, Argentina
    Plant Physiol 139:1291-303. 2005
    ..Here we show that the Arabidopsis (Arabidopsis thaliana) low vitamin C mutants, vtc1 and vtc2, which have between 10% and 25% of wild-type ascorbic acid, exhibit microlesions, express pathogenesis-related (PR) ..
  7. ncbi Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis
    Yuko Sasaki-Sekimoto
    Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Yokohama, Kanagawa, 226 8501, Japan
    Plant J 44:653-68. 2005
    ..Compared with the wild type, opr3 mutants were more sensitive to O3 exposure. These results suggest that the coordinated activation of the metabolic pathways mediated by jasmonates provides resistance to environmental stresses...
  8. ncbi Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana
    E Olmos
    Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    J Exp Bot 57:1645-55. 2006
    ..were studied in Arabidopsis thaliana mutants, which have constitutively moderately low (vtc1) or very low (vtc2) leaf AA contents compared with the wild type...
  9. pmc Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants
    Carole L Linster
    Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 282:18879-85. 2007
    ..Here we show that the protein encoded by VTC2, a gene mutated in vitamin C-deficient Arabidopsis thaliana strains, is a member of the GalT/Apa1 branch of the ..
  10. pmc The missing step of the L-galactose pathway of ascorbate biosynthesis in plants, an L-galactose guanyltransferase, increases leaf ascorbate content
    William A Laing
    Horticultural and Food Research Institute of New Zealand, PB 92160, Auckland 1142, New Zealand
    Proc Natl Acad Sci U S A 104:9534-9. 2007
    ..Transient expression of this A. chinensis gene in tobacco leaves resulted in a >3-fold increase in leaf ascorbate as well as a 50-fold increase in GDP-L-galactose-D-mannose-1-phosphate guanyltransferase activity...
  11. ncbi Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability
    John Dowdle
    School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
    Plant J 52:673-89. 2007
    ..We show that a GDP-L-galactose phosphorylase, encoded by the Arabidopsis thaliana VTC2 gene, catalyses this step in the ascorbate biosynthetic pathway...
  12. ncbi Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsis thaliana, with their interacting partners
    Yasunobu Ogura
    Division of Genome Analysis and Genetic Research, Life Science Research Center, Institute of Research Promotion, Kagawa University, 2393 Ikenobe, Miki cho, Kita gun, Kagawa 761 0795, Japan
    J Plant Res 121:97-105. 2008
    ..Yeast two-hybrid screening led to the isolation of VITAMIN C DEFECTIVE 2 (VTC2), VTC2-LIKE (VTC2L), and BEL1-LIKE HOMEODOMAIN 10 proteins (BLH10A and BLH10B) as PLP-interacting ..
  13. pmc A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction
    Carole L Linster
    Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 283:18483-92. 2008
    The Arabidopsis thaliana VTC2 gene encodes an enzyme that catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in the first committed step of the Smirnoff-Wheeler pathway to plant vitamin C synthesis...
  14. doi An expression analysis of the ascorbate biosynthesis enzyme VTC2
    Patricia Muller-Moule
    Developmental and Molecular Plant Biology, Heinrich Heine University, Universitatsstr 1, Dusseldorf, Germany
    Plant Mol Biol 68:31-41. 2008
    The vtc2 mutant of Arabidopsis thaliana is vitamin C deficient and is defective in the ascorbate biosynthesis enzyme GDP-L-Galactose phosphorylase/L -Galactose guanylyltransferase...
  15. pmc Glucosinolate metabolites required for an Arabidopsis innate immune response
    Nicole K Clay
    Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 323:95-101. 2009
    ..Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens...