WNVNY99_gp1

Summary

Gene Symbol: WNVNY99_gp1
Description: polyprotein
Species: West Nile virus

Top Publications

  1. ncbi [Immunochemical properties of short recombinant polypeptides of proteine of, the West Nile virus bearing epitopes of domains I and II]
    M V Bogachek
    Mol Biol (Mosk) 39:813-22. 2005
  2. pmc Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein
    Jojanneke Roosendaal
    School of Molecular and Microbial Sciences, University of Queensland, Coopers Road, St Lucia, Brisbane, Queensland 4072, Australia
    J Virol 80:4623-32. 2006
  3. pmc Crystal structure of the West Nile virus envelope glycoprotein
    Grant E Nybakken
    Department of Pathology and Immunology, Washington University School of Medicine, Campus Box 8118, 660 South Euclid Avenue, St Louis, MO 63110 1093, USA
    J Virol 80:11467-74. 2006
  4. ncbi Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters
    John D Morrey
    Institute for Antiviral Research, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322 4700, USA
    J Infect Dis 194:1300-8. 2006
  5. ncbi Characterization of the West Nile virus protease substrate specificity and inhibitors
    Niklaus H Mueller
    Department of Microbiology and Immunology, Georgetown University School of Medicine, 3900 Reservoir Road NW, Med Dent SW309, Washington, DC 20057, USA
    Int J Biochem Cell Biol 39:606-14. 2007
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
  7. ncbi Live-attenuated recombinant equine herpesvirus type 1 (EHV-1) induces a neutralizing antibody response against West Nile virus (WNV)
    Cristina T Rosas
    Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA
    Virus Res 125:69-78. 2007
  8. ncbi Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
    E G Westaway
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Virology 234:31-41. 1997
  9. pmc Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes
    Ryuta Kanai
    266Department of Molecular Biophysics and Biochemistry, The Bass Center for Structural Biology, Yale University, 266 Whitney Ave, New Haven, Connecticut 06520, USA
    J Virol 80:11000-8. 2006
  10. ncbi Induction of potent Th1-type immune responses from a novel DNA vaccine for West Nile virus New York isolate (WNV-NY1999)
    J S Yang
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104 6100, USA
    J Infect Dis 184:809-16. 2001

Scientific Experts

Detail Information

Publications71

  1. ncbi [Immunochemical properties of short recombinant polypeptides of proteine of, the West Nile virus bearing epitopes of domains I and II]
    M V Bogachek
    Mol Biol (Mosk) 39:813-22. 2005
    ..X-ray model of protein E let us suppose that bc-loop (73-89 aa) of domain II interacts with LBP and together with cd-loop (fusion peptide) determines an initial stages of penetration virions into cell...
  2. pmc Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein
    Jojanneke Roosendaal
    School of Molecular and Microbial Sciences, University of Queensland, Coopers Road, St Lucia, Brisbane, Queensland 4072, Australia
    J Virol 80:4623-32. 2006
    ....
  3. pmc Crystal structure of the West Nile virus envelope glycoprotein
    Grant E Nybakken
    Department of Pathology and Immunology, Washington University School of Medicine, Campus Box 8118, 660 South Euclid Avenue, St Louis, MO 63110 1093, USA
    J Virol 80:11467-74. 2006
    ..We speculate that E proteins could pivot around the fusion loop-pocket junction, allowing virion conformational transitions while minimizing fusion loop exposure...
  4. ncbi Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters
    John D Morrey
    Institute for Antiviral Research, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322 4700, USA
    J Infect Dis 194:1300-8. 2006
    ..These experiments suggest that humanized MAbs with potent neutralizing activity are a possible treatment for human patients after WNV has infected neurons in the central nervous system...
  5. ncbi Characterization of the West Nile virus protease substrate specificity and inhibitors
    Niklaus H Mueller
    Department of Microbiology and Immunology, Georgetown University School of Medicine, 3900 Reservoir Road NW, Med Dent SW309, Washington, DC 20057, USA
    Int J Biochem Cell Biol 39:606-14. 2007
    ....
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection...
  7. ncbi Live-attenuated recombinant equine herpesvirus type 1 (EHV-1) induces a neutralizing antibody response against West Nile virus (WNV)
    Cristina T Rosas
    Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA
    Virus Res 125:69-78. 2007
    ..From the results, we concluded that the EHV-1 vectored virus is able to express the WNV structural proteins and that vaccination of horses results in the induction of WNV E-protein-specific IgG(T), IgGb, and neutralizing antibodies...
  8. ncbi Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
    E G Westaway
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Virology 234:31-41. 1997
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  9. pmc Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes
    Ryuta Kanai
    266Department of Molecular Biophysics and Biochemistry, The Bass Center for Structural Biology, Yale University, 266 Whitney Ave, New Haven, Connecticut 06520, USA
    J Virol 80:11000-8. 2006
    ..By revealing the details of the molecular landscape of the West Nile virus surface, our structure will assist the design of antiviral vaccines and therapeutics...
  10. ncbi Induction of potent Th1-type immune responses from a novel DNA vaccine for West Nile virus New York isolate (WNV-NY1999)
    J S Yang
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104 6100, USA
    J Infect Dis 184:809-16. 2001
    ..These results support the potential utility of this method as a tool for developing immunization strategies for WNV and other emerging pathogens...
  11. ncbi The molecular biology of West Nile Virus: a new invader of the western hemisphere
    Margo A Brinton
    Department of Biology, Georgia State University, Atlanta 30303, USA
    Annu Rev Microbiol 56:371-402. 2002
    ..Aspects of virion structure, the viral replication cycle, viral protein function, genome structure, conserved viral elements, host factors, virus-host interactions, and vaccines are discussed in this review...
  12. pmc Envelope protein glycosylation status influences mouse neuroinvasion phenotype of genetic lineage 1 West Nile virus strains
    David W C Beasley
    Department of Pathology, Cancer Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas 77555 0609, USA
    J Virol 79:8339-47. 2005
    ..Therefore, the enhanced virulence of North American WNV strains compared with other Old World lineage 1 strains is at least partly mediated by envelope protein glycosylation...
  13. ncbi Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein
    J J H Chu
    Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
    J Gen Virol 86:405-12. 2005
    ..The data also suggest that similar attachment molecule(s) or receptor(s) were used by WNV and Den 2 virus for entry into C6/36 mosquito cells...
  14. pmc CD8+ T cells require perforin to clear West Nile virus from infected neurons
    Bimmi Shrestha
    Departments of Medicine, Molecular Microbiology, Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 80:119-29. 2006
    ..Taken together, our data suggest that despite the risk of immunopathogenesis, CD8+ T cells use a perforin-dependent mechanism to clear WNV from infected neurons...
  15. pmc West Nile virus 5'-cap structure is formed by sequential guanine N-7 and ribose 2'-O methylations by nonstructural protein 5
    Debashish Ray
    Wadsworth Center, New York State Department of Health, Albany, 12201, USA
    J Virol 80:8362-70. 2006
    ..These findings demonstrate a critical role for the flavivirus MTase in viral reproduction and underscore this domain as a potential target for antiviral therapy...
  16. pmc Isolation and characterization of human monoclonal antibodies from individuals infected with West Nile Virus
    Mark Throsby
    Crucell Holland B V, P O Box 2048, 2301 CA, Leiden, The Netherlands
    J Virol 80:6982-92. 2006
    ....
  17. ncbi A novel imidazole nucleoside containing a diaminodihydro-S-triazine as a substituent: inhibitory activity against the West Nile virus NTPase/helicase
    Ravi K Ujjinamatada
    Laboratory for Drug Design and Synthesis, Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, Maryland 21250, USA
    Nucleosides Nucleotides Nucleic Acids 24:1775-88. 2005
    ..Nucleoside 7 showed promising in vitro anti-helicase activity against the West Nile virus NTPase/helicase with an IC50 of 3-10 microg/mL...
  18. pmc Purification and crystallization of dengue and West Nile virus NS2B-NS3 complexes
    Allan D'arcy
    Novartis Institutes of Biomedical Research, Protease Platform, Klybeckstrasse 144, CH 4002 Basel, Switzerland
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:157-62. 2006
    ..Crystals that diffract to high resolution, suitable for three-dimensional structure determinations, have been obtained...
  19. pmc Nuclear localization of flavivirus RNA synthesis in infected cells
    Pradeep Devappa Uchil
    Department of Microbiology and Cell Biology, Room 254A, Sir C V Raman Avenue, Indian Institute of Science, Bangalore 560012, India
    J Virol 80:5451-64. 2006
    ..Thus, we conclusively demonstrate for the first time that the host cell nucleus functions as an additional site for the presence of functionally active flaviviral replicase complex...
  20. ncbi The kinetics of proinflammatory cytokines in murine peritoneal macrophages infected with envelope protein-glycosylated or non-glycosylated West Nile virus
    Kazuya Shirato
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Sapporo, 060 0818, Japan
    Virus Res 121:11-6. 2006
    ..Interferon (IFN) beta and IL-6 were up-regulated with the clearance of both viruses. These results suggest that TNFalpha and IL-1beta expression are related to the virulence of E protein-glycosylated WN virus...
  21. pmc A single amino acid substitution in the West Nile virus nonstructural protein NS2A disables its ability to inhibit alpha/beta interferon induction and attenuates virus virulence in mice
    Wen Jun Liu
    School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Australia
    J Virol 80:2396-404. 2006
    ....
  22. ncbi Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus
    Paul Erbel
    Novartis Institutes for BioMedical Research, Protease Platform, 4002 Basel, Switzerland
    Nat Struct Mol Biol 13:372-3. 2006
    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
  23. doi West Nile virus capsid protein interaction with importin and HDM2 protein is regulated by protein kinase C-mediated phosphorylation
    Raghavan Bhuvanakantham
    Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Microbes Infect 12:615-25. 2010
    ..Collectively, this study highlighted that phosphorylation is an important post-translational modification required to execute the functions of C protein...
  24. doi Specific interaction of capsid protein and importin-alpha/beta influences West Nile virus production
    Raghavan Bhuvanakantham
    Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
    Biochem Biophys Res Commun 389:63-9. 2009
    ....
  25. ncbi Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease
    Aloke K Bera
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:12883-92. 2007
    ..Formation of an active protease was found to require neither cleavage of NS2B from NS3 nor a free NS3 N terminus. Evidence was also obtained for product inhibition of the protease by the cleaved C terminus of NS2B...
  26. pmc Switching the substrate specificity of the two-component NS2B-NS3 flavivirus proteinase by structure-based mutagenesis
    Sergey A Shiryaev
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, USA
    J Virol 81:4501-9. 2007
    ....
  27. pmc Expression and purification of a two-component flaviviral proteinase resistant to autocleavage at the NS2B-NS3 junction region
    Sergey A Shiryaev
    Inflammatory and Infectious Disease Center, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Protein Expr Purif 52:334-9. 2007
    ..This construct exhibits high stability and functional activity and is thus well suited for the follow-up purification and structural and drug design studies...
  28. pmc Cleavage preference distinguishes the two-component NS2B-NS3 serine proteinases of Dengue and West Nile viruses
    Sergey A Shiryaev
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Biochem J 401:743-52. 2007
    ..We believe that our data represent a valuable biochemical resource and a solid foundation to support the design of selective substrates and synthetic inhibitors of flaviviral proteinases...
  29. ncbi Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep
    Josh D Boone
    Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Vaccine 25:672-8. 2007
    ..These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines...
  30. pmc Preparation and immunogenic properties of a recombinant West Nile subunit vaccine
    Michael M Lieberman
    Hawaii Biotech, Inc, 99 193 Aiea Heights Drive, Aiea, HI 96701, USA
    Vaccine 25:414-23. 2007
    ..These findings are encouraging and warrant further in vivo studies to determine the protective efficacy of the WNV vaccine candidate...
  31. pmc The infectivity of prM-containing partially mature West Nile virus does not require the activity of cellular furin-like proteases
    Swati Mukherjee
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, 33 North Drive, Building 33, Room 2E19A 2, Bethesda, MD 20892, USA
    J Virol 85:12067-72. 2011
    ..Thus, partially mature WNV does not require furin-like proteases for infectivity...
  32. pmc Pathogenesis of West Nile Virus infection: a balance between virulence, innate and adaptive immunity, and viral evasion
    Melanie A Samuel
    Division of Infectious Diseases, Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:9349-60. 2006
  33. pmc The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus
    Zaikun Xu
    Department of Cell Biology, University of Alberta, Edmonton, Canada
    J Virol 85:5571-80. 2011
    ..Together, these data suggest that DDX56 is required for assembly of infectious WNV particles...
  34. ncbi Biological properties of chimeric West Nile viruses
    Victoria Borisevich
    Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Ave, Lawrence, KS 66045, USA
    Virology 349:371-81. 2006
    ..Chimeric viruses carrying the NY99 structural proteins were attenuated in neuroinvasiveness and demonstrated an immunogenicity superior to W956...
  35. pmc NS1 protein secretion during the acute phase of West Nile virus infection
    Joanne Macdonald
    Jerome L and Dawn Greene Infectious Disease Laboratory, Department of Epidemiology, Mailman School of Public Health, Columbia University, 722 W 168th St, Rm 1801, New York, NY 10032, USA
    J Virol 79:13924-33. 2005
    ..Moreover, the 4G4-ACE was found to be superior in performance to both the IgM and plaque assays during this time period, suggesting that NS1 is a viable early diagnostic marker of WNV infection...
  36. ncbi Mouse neuroinvasive phenotype of West Nile virus strains varies depending upon virus genotype
    David W C Beasley
    Department of Pathology, The University of Texas Medical Branch, Galveston, Texas 77555 0609, USA
    Virology 296:17-23. 2002
    ..Virus isolated in North America was found to be highly neuroinvasive with a lack of age-related resistance to infection in mice normally associated with mosquito-borne flaviviruses...
  37. ncbi Inhibition of the helicase activity of HCV NTPase/helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5 '-triphosphate (ribavirin-TP)
    P Borowski
    Abteilung fur Virologie, Bernhard Nocht Institut fur Tropenmedizin, Hamburg, Germany
    Acta Biochim Pol 48:739-44. 2001
    ..Our results suggest that derivatives of ribavirin-TP with enhanced stability towards hydrolytic attack may be effective inhibitors of the enzyme...
  38. ncbi West Nile virus strains differ in mouse neurovirulence and binding to mouse or human brain membrane receptor preparations
    D W Beasley
    WHO Collaborating Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston 77555 0609, USA
    Ann N Y Acad Sci 951:332-5. 2001
  39. ncbi West Nile virus-induced bax-dependent apoptosis
    M C Parquet
    Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1 12 4 Sakamoto, Nagasaki 852 8523, Japan
    FEBS Lett 500:17-24. 2001
    ..Additionally, signals involved in WNV-induced apoptosis are associated with the up-regulation of bax gene expression...
  40. ncbi Expression and purification of enzymatically active recombinant RNA-dependent RNA polymerase (NS5) of the flavivirus Kunjin
    K J Guyatt
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    J Virol Methods 92:37-44. 2001
    ..However, in contrast to RNA polymerases of other Flavivirus species, purified KUN NS5 polymerase produced a single, full-length replicon RNA product, thus demonstrating efficient processivity...
  41. ncbi Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro
    R Yusof
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Biol Chem 275:9963-9. 2000
    ..These results suggest that heterodimerization of the NS3pro domain with NS2B generates additional specific interactions with the P2 and P3 residues of the substrates...
  42. pmc cis- and trans-acting elements in flavivirus RNA replication
    A A Khromykh
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Queensland 4029, Australia
    J Virol 74:3253-63. 2000
    ....
  43. ncbi Homology model of the dengue 2 virus NS3 protease: putative interactions with both substrate and NS2B cofactor
    R I Brinkworth
    Centre for Drug Design and Development, University of Queensland, St Lucia, Brisbane, Australia
    J Gen Virol 80:1167-77. 1999
    ....
  44. ncbi West Nile virus envelope proteins: nucleotide sequence analysis of strains differing in mouse neuroinvasiveness
    T J Chambers
    Department of Molecular Microbiology and Immunology, St Louis University School of Medicine, MO 63104, USA
    J Gen Virol 79:2375-80. 1998
    ..Also, sequence comparison with the WN virus (Nigeria) strain revealed considerable divergence of the E protein at the nucleotide and amino acid levels...
  45. pmc Cell proteins bind specifically to West Nile virus minus-strand 3' stem-loop RNA
    P Y Shi
    Department of Biology, Georgia State University, Atlanta 30303, USA
    J Virol 70:6278-87. 1996
    ..It is possible that the increased binding activity of one of the resistant cell proteins for the flavivirus minus-strand RNA could result in a reduced synthesis of plus-strand RNA as observed with the flavivirus resistance phenotype...
  46. ncbi The NS 3 nonstructural protein of flaviviruses contains an RNA triphosphatase activity
    G Wengler
    Institut fur Virologie, Justus Liebig Universitat Giessen, Germany
    Virology 197:265-73. 1993
    ..An amino acid sequence element which may be involved in recognition of the 5'-terminal RNA triphosphate is tentatively identified. A homologous element may be present in the vaccinia virus-coded RNA triphosphatase...
  47. pmc BHK cell proteins that bind to the 3' stem-loop structure of the West Nile virus genome RNA
    J L Blackwell
    Department of Biology, Georgia State University, Atlanta 30303, USA
    J Virol 69:5650-8. 1995
    ..The 3'-terminal SL RNA of another flavivirus, dengue virus type 3, specifically competed with the WNV (+)3'SL RNA in gel shift assays, suggesting that the host proteins identified in this study are flavivirus specific...
  48. ncbi Separation of functional West Nile virus replication complexes from intracellular membrane fragments
    J B Grun
    Wistar Institute, Philadelphia, Pennsylvania 19104
    J Gen Virol 69:3121-7. 1988
    ..Of the five detergents (Tween 20, maltoside, octylglucoside, lubrol PX and sodium deoxycholate) tested, sodium deoxycholate was the most efficient at releasing functional viral replication complexes from intracellular membranes...
  49. pmc Cell-associated West Nile flavivirus is covered with E+pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during virus release
    G Wengler
    Institut fur Virologie, Justus Liebig Universitat, Giessen, Federal Republic of Germany
    J Virol 63:2521-6. 1989
    ..Preparations of cell-associated virus contained approximately 40,000 physical particles for each PFU...
  50. pmc N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases
    A E Gorbalenya
    Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region
    Nucleic Acids Res 17:3889-97. 1989
    ..The protease domain is probably involved in the processing of viral non-structural proteins...
  51. pmc Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly
    Wen Jun Liu
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland 4029, Australia
    J Virol 76:10766-75. 2002
    ..The results define minimal regions in the NS3 and NS5 genes essential for the formation of complementable replication complex and show a requirement of NS3 in cis for virus assembly...
  52. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  53. pmc Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
    Theodore Oliphant
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, Missouri 63110, USA
    Nat Med 11:522-30. 2005
    ..In postexposure therapeutic trials in mice, a single dose of humanized E16 protected mice against WNV-induced mortality, and may therefore be a viable treatment option against WNV infection in humans...
  54. ncbi Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
    Kazuya Shirato
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060 0818, Japan
    J Gen Virol 85:3637-45. 2004
    ..These results suggested that E protein glycosylation is a molecular determinant of neuroinvasiveness in the NY strains of WN virus...
  55. ncbi Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells
    Justin Jang Hann Chu
    Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597
    J Biol Chem 279:54533-41. 2004
    ..The identification of alpha(v)beta(3) integrin as a receptor for WNV provides insight into virus-receptor interaction, hence creating opportunities in the development of anti-viral strategies against WNV infection...
  56. pmc Solution structure of dengue virus capsid protein reveals another fold
    Lixin Ma
    Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 101:3414-9. 2004
    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
  57. pmc Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase
    Sergey A Shiryaev
    The Burnham Institute, La Jolla, CA 92037, USA
    Biochem J 393:503-11. 2006
    ..Overall, our findings represent a foundation for in-depth mechanistic and structural studies as well as for the design of novel and efficient inhibitors of WNV NS3...
  58. ncbi Chimeric flaviviruses: novel vaccines against dengue fever, tick-borne encephalitis, and Japanese encephalitis
    CHING JUH LAI
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 61:469-509. 2003
    ..The chimeric flavivirus strategy has led to the rapid development of novel live-attenuated vaccines against dengue, TBE, JE, and West Nile viruses...
  59. ncbi Requirements for West Nile virus (-)- and (+)-strand subgenomic RNA synthesis in vitro by the viral RNA-dependent RNA polymerase expressed in Escherichia coli
    Masako Nomaguchi
    Department of Microbiology and Immunology, Georgetown University Medical Center, SW309 Med Dent Building, 3900 Reservoir Road, Washington, D C 20057, USA
    J Biol Chem 279:12141-51. 2004
    ..These results support a model that "cyclization" of the viral RNA play a role for (-)-strand RNA synthesis but not for (+)-strand RNA synthesis...
  60. ncbi Antigenic structure of flavivirus proteins
    John T Roehrig
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
    Adv Virus Res 59:141-75. 2003
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  61. pmc Unexpected similarity between the cytosolic West Nile virus NS3 and the secretory furin-like serine proteinases
    Nabil G Seidah
    Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, QC, H2W 1R7, Canada
    Biochem J 393:e1-3. 2006
    ..Structural modelling based on Dengue virus NS3 provided a possible rationale for the observed cleavage specificity of West Nile virus NS3...
  62. ncbi Energetics of RNA binding by the West Nile virus RNA triphosphatase
    Ines Benzaghou
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, 3001 12e Avenue, Sherbrooke, Queb, Canada J1H 5N4
    FEBS Lett 580:867-77. 2006
    ..Our data indicate that the protein undergoes structural modifications upon RNA binding, although the interaction does not significantly modify the stability of the protein...
  63. pmc West Nile virus-induced neuroinflammation: glial infection and capsid protein-mediated neurovirulence
    Guido van Marle
    Departments of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    J Virol 81:10933-49. 2007
    ..These studies underscore the important interactions between the WNV capsid protein and neuroinflammation in the pathogenesis of WNV-induced neurological disorders...
  64. pmc Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
    Eloise Mastrangelo
    Department of Biomolecular Sciences and Biotechnology, CNR INFM, University of Milano, Via Celoria 26, 20133 Milano, Italy
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:876-9. 2006
    ..Kunjin virus NS3 helicase domain is an attractive model for studying the molecular mechanisms of flavivirus replication, while simultaneously providing a new basis for the rational development of anti-flaviviral compounds...
  65. pmc Chimeric dengue 2 PDK-53/West Nile NY99 viruses retain the phenotypic attenuation markers of the candidate PDK-53 vaccine virus and protect mice against lethal challenge with West Nile virus
    Claire Y H Huang
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, P O Box 2087, Fort Collins, CO 80522, USA
    J Virol 79:7300-10. 2005
    ....
  66. ncbi Expression and evolutionary analysis of West Nile virus (Merion strain)
    Mathura P Ramanathan
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Neurovirol 11:544-56. 2005
    ..Evolutionary analysis of Merion strain sequences indicates that this strain is distinct phylogenetically from the previously reported WNV strains...
  67. pmc CD4+ T-cell responses are required for clearance of West Nile virus from the central nervous system
    Elizabeth M Sitati
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:12060-9. 2006
    ..Our experiments suggest that the dominant protective role of CD4(+) T cells during primary WNV infection is to provide help for antibody responses and sustain WNV-specific CD8(+) T-cell responses in the CNS that enable viral clearance...
  68. pmc Production and characterization of vaccines based on flaviviruses defective in replication
    Peter W Mason
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1019, USA
    Virology 351:432-43. 2006
    ..Such defective flaviviruses can be produced in large scale under low biocontainment conditions and should be useful for diagnostic or vaccine applications...
  69. ncbi West Nile virus neuroinvasive disease
    Larry E Davis
    Neurology Services, New Mexico Veterans Affairs Health Care System, University of New Mexico, Albuquerque, NM, USA
    Ann Neurol 60:286-300. 2006
    ..Recovery from neurological sequelae of WNV infection including cognitive deficits and weakness may be prolonged and incomplete...

Research Grants9

  1. Development of Kunjin replicon-based Ebloa vaccine
    Alexander Khromykh; Fiscal Year: 2004
    ..abstract_text> ..
  2. Oas-1 gene transgenic mice for WNV research.
    MARGO BRINTON; Fiscal Year: 2005
    ....
  3. Proprotein Processing, Trafficking and Secretion Gordon Conference
    Nabil Seidah; Fiscal Year: 2006
    ..The integration of protease systems that are required both for normal physiology and human disease will be relevant to our understanding of the molecular bases of diabetes, cancer, Alzheimer's and drug addiction. ..
  4. Analysis of SNPs Associated With WNV-Induced Disease
    MARGO BRINTON; Fiscal Year: 2006
    ..Further analyses will include fine mapping and sequencing of the causative mutation(s). ..
  5. West Nile Virus Life Cycle in Flavivirus-Resistant Cells
    Pei Yong Shi; Fiscal Year: 2006
    ..abstract_text> ..
  6. Attenuated West Nile virus envelope protein mutants
    David Beasley; Fiscal Year: 2006
    ..These studies will identify E protein residues that can impart an attenuated phenotype and these will be exploited for WNV vaccine development in a subsequent R01 grant application. ..
  7. Structure and size determination of S. aureus pathogenicity island capsids
    Terje Dokland; Fiscal Year: 2008
    ..The bacteriophage/pathogenicity island system is of special interest for its role in transferring toxin genes and conferring pathogenicity on non-pathogenic strains. [unreadable] [unreadable] [unreadable]..
  8. Development of a new model of viral hemorrhagic fever.
    MARGO BRINTON; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  9. Functional analysis of flavivirus genetic resistance.
    Margo A Brinton; Fiscal Year: 2010
    ..This research is relevant to understanding individual variation in the response to flavivirus infections among humans. ..