Liver Cell Membrane Protein-Expression and Function

Summary

Principal Investigator: Allan W Wolkoff
Abstract: This is a Program Project to continue investigation of the functions and trafficking of specific liver cell membrane proteins. Four projects involving 15 faculty investigators representing 6 academic departments are proposed. These projects share ideas, techniques, and investigators who meet often, both formally and informally. The proposed studies represent multidisciplinary approaches to several fundamental questions in liver pathobiology including mechanisms of trafficking of membrane proteins on specific cytoskeletal elements (Project 1), the regulation of expression and trafficking of connexins (Project 2), the identification of trafficking steps in endocytosis mutants (Project 3), and mechanisms of trafficking to autophagic vacuoles and lysosomes (Project 4). Project 4 is new and was added to the Program because of strong collaborations that developed with the existing three Projects. Major accomplishments over the past 5 year period include: (Project 1) reconstitution in vitro of the microtubule-based motility of early and late endocytic vesicles as well as exocytosing vesicles that contain Herpes simplex virus and identification of vesicle-associated motors and regulatory factors;(Project 2) identification and characterization of protein binding partners for connexin 32 that may regulate its trafficking to and from the plasma membrane;(Project 3) discovery that the association of a potential sorting heat shock protein heterocomplex with the phosphorylated asialoglycoprotein receptor cytoplasmic domain is mediated by a novel casein kinase 2 alpha subunit, CK2a". This Program also includes three Core facilities. The Administrative and Supporting Services Core (Core A) provides secretarial, bookkeeping, and common equipment functions. The Molecular Cytology Core (Core B) provides state-of-the-art microscopy and imaging services. A new Proteomics Core (Core C) has been added based upon the substantial use of this technology by each of the four Projects. All four Projects are concerned with interrelated areas of hepatocyte membrane biology and pathobiology, and each proposed project represents collaborative efforts between independent investigators. Collaborations within and between projects lead to extensions of an individual's expertise into important areas of liver pathobiology that could not otherwise be effectively investigated. The track record demonstrates the ability of the investigators involved to work together and interact synergistically within the framework of this Program to promote the sharing of ideas, methodology, and resources. Ultimately, the studies performed in this Program will lead to fundamental insights that should be important for understanding and treating or preventing various acquired and inherited disorders of the liver.
Funding Period: -----------------199 - ----------------2013
more information: NIH RePORT

Top Publications

  1. ncbi Autophagy and lipids: tightening the knot
    Jose Antonio Rodriguez-Navarro
    Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Semin Immunopathol 32:343-53. 2010
  2. pmc UL36p is required for efficient transport of membrane-associated herpes simplex virus type 1 along microtubules
    Sara K Shanda
    Department of Developmental and Molecular Biology, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 82:7388-94. 2008
  3. pmc The chaperone-mediated autophagy receptor organizes in dynamic protein complexes at the lysosomal membrane
    Urmi Bandyopadhyay
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 28:5747-63. 2008
  4. pmc Restoration of chaperone-mediated autophagy in aging liver improves cellular maintenance and hepatic function
    Cong Zhang
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Research, 1300 Morris Park Avenue, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Med 14:959-65. 2008
  5. pmc IGF-I regulates tight-junction protein claudin-1 during differentiation of osteoblast-like MC3T3-E1 cells via a MAP-kinase pathway
    Naoko Hatakeyama
    Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, S1, W17, Sapporo, 060 8556, Japan
    Cell Tissue Res 334:243-54. 2008
  6. pmc Entering the lysosome through a transient gate by chaperone-mediated autophagy
    Urmi Bandyopadhyay
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY, New York 10461, USA
    Autophagy 4:1101-3. 2008
  7. pmc Autophagy and aging: keeping that old broom working
    Ana Maria Cuervo
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Studies, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Ullmann B 611, Bronx, NY 10461, USA
    Trends Genet 24:604-12. 2008
  8. pmc The neuronal connexin36 interacts with and is phosphorylated by CaMKII in a way similar to CaMKII interaction with glutamate receptors
    Cantas Alev
    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, 44781 Bochum, Germany
    Proc Natl Acad Sci U S A 105:20964-9. 2008
  9. pmc Human liver cell trafficking mutants: characterization and whole exome sequencing
    Fei Yuan
    Marion Bessin Liver Research Center, Albert Einstein College of Medicine, New York, New York, United States of America Department of Cell Biology, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 9:e87043. 2014
  10. pmc Oatp1a1 requires PDZK1 to traffic to the plasma membrane by selective recruitment of microtubule-based motor proteins
    Wen Jun Wang
    Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Division of Gastroenterology and Liver Diseases, Albert Einstein College of Medicine, Bronx, New York
    Drug Metab Dispos 42:62-9. 2014

Scientific Experts

  • ANA M CUERVO
  • Jihyeon Lim
  • John W Murray
  • Rajat Singh
  • Stine Falsig Pedersen
  • Alfredo G Fort
  • Erik Lee Snapp
  • Oscar K Nihei
  • Aaron J Bell
  • Susmita Kaushik
  • David C Spray
  • Allan W Wolkoff
  • Hiroshi Koga
  • Eloy Bejarano
  • Esther Wong
  • Lindsey M Costantini
  • Urmi Bandyopadhyay
  • Ashish C Massey
  • Peter Satir
  • Sangeeta Nath
  • Richard J Stockert
  • Elvira S Cannizzo
  • Ranjit Sahu
  • Esperanza Arias
  • Cong Zhang
  • Takashi Kojima
  • Souvik Sarkar
  • Fei Yuan
  • Wen Jun Wang
  • Bindi Patel
  • Andrea Yuste
  • Miriam Windsor
  • Laura Santambrogio
  • Cristina C Clement
  • Edward Nieves
  • Aparna Mukhopadhyay
  • Pamela Stanley
  • Barry Potvin
  • Jose Antonio Rodriguez-Navarro
  • Marta Martinez-Vicente
  • Samantha J Orenstein
  • S Kaushik
  • Maria Kon
  • Antonia Follenzi
  • Mickael Derangeon
  • Linda Schneider
  • Naoko Hatakeyama
  • Sara K Shanda
  • Enko N Kiprilov
  • Søren Tvorup Christensen
  • Yongjun Wang
  • Cantas Alev
  • Norimasa Sawada
  • Rolf Dermietzel
  • Masaki Murata
  • Duncan W Wilson
  • Heather S Duffy
  • Eustratios Bananis
  • Donglin Bai
  • Tianmin Huang
  • Grace E Lee
  • Randy F Stout
  • Sylvia O Suadicani
  • Phyllis M Novikoff
  • Matías Jaureguiberry-Bravo
  • Oksana M Subach
  • Vladislav V Verkhusha
  • Larissa N Almeida
  • Paul Monaghan
  • Fernando Macian
  • Paulo Pereira
  • Kateryna Morozova
  • Thomas Wileman
  • Carlo Follo
  • Carla Marques
  • Philippa Hawes
  • Rut Valdor
  • Maria L Salas
  • Henrique Girao
  • Matteo Fossati
  • Maura Francolini
  • Javier M Rodriguez
  • Kristie Gordon
  • Ruth Hogue Angeletti
  • Lianji Jin
  • Brian Scharf
  • Ilaria Potolicchio
  • Jean Wang
  • Fa Yun Che
  • Spike Harris

Detail Information

Publications58

  1. ncbi Autophagy and lipids: tightening the knot
    Jose Antonio Rodriguez-Navarro
    Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Semin Immunopathol 32:343-53. 2010
    ..In addition, we comment on the regulatory role that lipid molecules and their modifying enzymes play on different steps of the autophagic process...
  2. pmc UL36p is required for efficient transport of membrane-associated herpes simplex virus type 1 along microtubules
    Sara K Shanda
    Department of Developmental and Molecular Biology, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 82:7388-94. 2008
    ..This decrease in binding and motility was restored when UL36p was supplied in trans by a complementing cell line. These findings suggest that UL36p is necessary for HSV-1 anterograde transport...
  3. pmc The chaperone-mediated autophagy receptor organizes in dynamic protein complexes at the lysosomal membrane
    Urmi Bandyopadhyay
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 28:5747-63. 2008
    ..Thus, we have identified a novel function for hsc70 in the disassembly of LAMP-2A from these complexes, whereas the presence of lysosome-associated hsp90 is essential to preserve the stability of LAMP-2A at the lysosomal membrane...
  4. pmc Restoration of chaperone-mediated autophagy in aging liver improves cellular maintenance and hepatic function
    Cong Zhang
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Research, 1300 Morris Park Avenue, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Med 14:959-65. 2008
    ....
  5. pmc IGF-I regulates tight-junction protein claudin-1 during differentiation of osteoblast-like MC3T3-E1 cells via a MAP-kinase pathway
    Naoko Hatakeyama
    Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, S1, W17, Sapporo, 060 8556, Japan
    Cell Tissue Res 334:243-54. 2008
    ....
  6. pmc Entering the lysosome through a transient gate by chaperone-mediated autophagy
    Urmi Bandyopadhyay
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY, New York 10461, USA
    Autophagy 4:1101-3. 2008
    ..The possible advantages of this transitory lysosomal translocon are discussed in light of the unique properties of the lysosomal compartment...
  7. pmc Autophagy and aging: keeping that old broom working
    Ana Maria Cuervo
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Studies, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Ullmann B 611, Bronx, NY 10461, USA
    Trends Genet 24:604-12. 2008
    ..Here, I review the recent genetic evidence in support of tight connections between autophagy, health span and aging...
  8. pmc The neuronal connexin36 interacts with and is phosphorylated by CaMKII in a way similar to CaMKII interaction with glutamate receptors
    Cantas Alev
    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, 44781 Bochum, Germany
    Proc Natl Acad Sci U S A 105:20964-9. 2008
    ..In analogy to the current notion of NR2B interaction with CaMKII, we propose a model that provides a mechanistic framework for CaMKII and Cx36 interaction at electrical synapses...
  9. pmc Human liver cell trafficking mutants: characterization and whole exome sequencing
    Fei Yuan
    Marion Bessin Liver Research Center, Albert Einstein College of Medicine, New York, New York, United States of America Department of Cell Biology, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 9:e87043. 2014
    ..In addition, the Ile34Phe mutation reduced both guanine nucleotide binding and hydrolysis activities of RAB22A. Thus, the RAB22A Ile34Phe mutation appears to contribute to the Trf4 mutant phenotype. ..
  10. pmc Oatp1a1 requires PDZK1 to traffic to the plasma membrane by selective recruitment of microtubule-based motor proteins
    Wen Jun Wang
    Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Division of Gastroenterology and Liver Diseases, Albert Einstein College of Medicine, Bronx, New York
    Drug Metab Dispos 42:62-9. 2014
    ..Whether this is a result of direct interaction of the Oatp1a1 cytoplasmic domain with dynein or with a dynein-containing protein complex remains to be established. ..
  11. pmc Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway
    Lindsey M Costantini
    Department Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Biochem Biophys Res Commun 430:1114-9. 2013
    ..We report successful creation of a secretory pathway-optimized blue FP, secBFP2...
  12. pmc Age-related oxidative stress compromises endosomal proteostasis
    Elvira S Cannizzo
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell Rep 2:136-49. 2012
    ..These findings underscore a previously unrecognized negative effect of age-dependent changes in cellular proteostasis on the immune response...
  13. pmc Integration of clearance mechanisms: the proteasome and autophagy
    Esther Wong
    Department of Developmental and Molecular Biology, Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cold Spring Harb Perspect Biol 2:a006734. 2010
    ....
  14. pmc Connexins modulate autophagosome biogenesis
    Eloy Bejarano
    Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Cell Biol 16:401-14. 2014
    ..Maturation of the Cx-containing pre-autophagosomes into autophagosomes leads to degradation of these endogenous inhibitors, allowing for sustained activation of autophagy. ..
  15. pmc Constitutive activation of chaperone-mediated autophagy in cells with impaired macroautophagy
    Susmita Kaushik
    Departments of Anatomy and Structural Biology and Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biol Cell 19:2179-92. 2008
    ..This work supports a direct cross-talk between these two forms of autophagy, and it identifies changes in the lysosomal compartment that underlie the basis for the communication between both autophagic pathways...
  16. pmc Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery
    Enko N Kiprilov
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 180:897-904. 2008
    ..These findings show that hESCs contain primary cilia associated with working Hh machinery...
  17. ncbi PKCzeta is required for microtubule-based motility of vesicles containing the ntcp transporter
    Souvik Sarkar
    Marion Bessin Liver Research Center, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Traffic 7:1078-91. 2006
    ..These data indicate that PKCzeta is required specifically for the intracellular movement of vesicles that contain the ntcp transporter...
  18. pmc Reconstitution of herpes simplex virus microtubule-dependent trafficking in vitro
    Grace E Lee
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Virol 80:4264-75. 2006
    ..This simple, minimal reconstitution of microtubule-mediated anterograde traffic should facilitate and complement molecular analysis of HSV egress in vivo...
  19. ncbi Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB)
    Donglin Bai
    Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    J Pharmacol Exp Ther 319:1452-8. 2006
    ..The differential efficacy of block by 2-APB of gap junction channels formed by different connexins may provide a useful tool that could be exploited in gap junction research to selectively block certain gap junction channel subtypes...
  20. ncbi Overview of structure and function of mammalian cilia
    Peter Satir
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    Annu Rev Physiol 69:377-400. 2007
    ..Unique motile 9 + 0 cilia, found during development at the embryonic node, determine left-right asymmetry of the body...
  21. ncbi The gap junction protein connexin32 interacts with the Src homology 3/hook domain of discs large homolog 1
    Heather S Duffy
    Department of Pharmacology, Columbia University, New York, New York 10032, USA
    J Biol Chem 282:9789-96. 2007
    ..Together, these results suggest that loss of Cx32 alters the levels, localization, and interactions of the tumor suppressor protein Dlgh1, events known in other systems to alter cell cycle and increase tumorigenicity...
  22. pmc Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver
    Sangeeta Nath
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biol Cell 18:1839-49. 2007
    ..However, the present study shows that coordinated activity of these kinesins is essential for motility and processing of early endocytic vesicles...
  23. pmc New liver cell mutants defective in the endocytic pathway
    Richard J Stockert
    The Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochim Biophys Acta 1768:1741-9. 2007
    ..Identifying the biochemical and genetic mechanisms underlying these phenotypes should uncover novel and unpredicted protein-protein or protein-lipid interactions that orchestrate specific steps in membrane protein trafficking...
  24. ncbi Adaptor heat shock protein complex formation regulates trafficking of the asialoglycoprotein receptor
    Tianmin Huang
    Albert Einstein College of Medicine, 1300 Morris Park Ave, Liver Research Center, Ullmann 611, Bronx, NY 10416, USA
    Am J Physiol Gastrointest Liver Physiol 290:G369-76. 2006
    ..The data presented provide evidence that recruitment of AP1 and AP2, which is necessary for appropriate receptor trafficking, is mediated by the interaction of AP with the ASGPR-CD-bound HSP complex...
  25. ncbi Connexins induce and maintain tight junctions in epithelial cells
    Takashi Kojima
    Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan
    J Membr Biol 217:13-9. 2007
    ..These results suggest that connexins can induce and maintain tight junctions in both GJIC-dependent and -independent manners in epithelial cells...
  26. ncbi Pathology of mechanical and gap junctional co-coupling at the intercalated disc: Is sepsis a junctionopathy?
    David C Spray
    Crit Care Med 35:2231-2. 2007
  27. pmc Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus
    Yongjun Wang
    Department of Medicine, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Biol Chem 283:4766-77. 2008
    ....
  28. ncbi Early cellular changes after blockage of chaperone-mediated autophagy
    Ashish C Massey
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Autophagy 4:442-56. 2008
    ..Based on the time-course of the cellular changes, we propose that a minimal threshold of these toxic products needs to accumulate in order to constitutively activate macroautophagy and thus return cellular homeostasis to normal...
  29. ncbi Single vesicle analysis of endocytic fission on microtubules in vitro
    John W Murray
    Marion Bessin Liver Research Center and Department of Medicine, and Division of Hepatology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Traffic 9:833-47. 2008
    ..A single round of fission resulted in 6.5-fold purification of ntcp from ASOR, and 25% of the resulting vesicles were completely depleted of the endocytic ligand...
  30. pmc Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles
    Aparna Mukhopadhyay
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Sci 124:765-75. 2011
    ....
  31. pmc Chaperone-mediated autophagy in protein quality control
    Esperanza Arias
    Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Curr Opin Cell Biol 23:184-9. 2011
    ..In fact, CMA activation seems to be a common mechanism of cellular defense against proteotoxicity...
  32. pmc Heterogeneous accumulation of fluorescent bile acids in primary rat hepatocytes does not correlate with their homogenous expression of ntcp
    John W Murray
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Gastrointest Liver Physiol 301:G60-8. 2011
    ..These studies indicate that single-cell imaging can provide insight into previously unrecognized details of anion transport in the complex environment of polarized hepatocytes...
  33. pmc Autophagy in the cellular energetic balance
    Rajat Singh
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell Metab 13:495-504. 2011
    ..This recently discovered interplay between autophagy and lipid and carbohydrate metabolism reveals the existence of a dynamic feedback between autophagy and cellular energy balance...
  34. pmc In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors
    Alfredo G Fort
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 286:22875-85. 2011
    ....
  35. pmc Frozen tissue can provide reproducible proteomic results of subcellular fractionation
    Jihyeon Lim
    Laboratory for Macromolecular Analysis and Proteomics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Anal Biochem 418:78-84. 2011
    ..This demonstrates the feasibility of analyzing cellular compartment-specific proteins in archived tissue samples with the simple DDF method...
  36. pmc Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions
    Lindsey M Costantini
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Traffic 13:643-9. 2012
    ..We tested three FPs and identified two as sufficiently monomeric, while a third previously reported as monomeric was found to strongly oligomerize...
  37. pmc Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner
    Eloy Bejarano
    Department of Development and Molecular Biology, Albert Einstein College of Medicine, New York, NY 10461, USA
    Mol Biol Cell 23:2156-69. 2012
    ..This study reveals a novel regulatory role for macroautophagy in GJ function that is directly dependent on the ubiquitinylation of plasma membrane connexins...
  38. pmc Microautophagy of cytosolic proteins by late endosomes
    Ranjit Sahu
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Cell 20:131-9. 2011
    ..Therefore, we propose that endosomal microautophagy shares molecular components with both the endocytic and autophagic pathways...
  39. pmc Mechanism of collapse of endoplasmic reticulum cisternae during African swine fever virus infection
    Miriam Windsor
    Institute for Animal Health, Pirbright Laboratory, Surrey, UK
    Traffic 13:30-42. 2012
    ..This provides a novel mechanism for biogenesis of modified stacks of ER present in cells infected with ASFV, and may also be relevant to cellular processes...
  40. pmc Autophagic pathways and metabolic stress
    S Kaushik
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Diabetes Obes Metab 12:4-14. 2010
    ..We also comment on the consequences that chronic exposure to these metabolic stressors could have on autophagic function and on how this effect may underlie the basis of some common metabolic disorders...
  41. ncbi Methods to monitor chaperone-mediated autophagy
    Susmita Kaushik
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Institute for Aging Research, Albert Einstein College of Medicine, Bronx, New York, USA
    Methods Enzymol 452:297-324. 2009
    ..In this chapter we review the different experimental approaches used to assess CMA activity both in cells in culture and in different organs from animals...
  42. pmc Trifluoroethanol reveals helical propensity at analogous positions in cytoplasmic domains of three connexins
    Alfredo G Fort
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA
    Biopolymers 92:173-82. 2009
    ..This first comparative study of conformational properties of connexin cytoplasmic domains reveals protein domains that may play similar roles in channel function and protein-protein interactions...
  43. pmc GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila
    Aaron J Bell
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA
    Cell Motil Cytoskeleton 66:483-99. 2009
    ..GEF2-HA localized near basal bodies but not to cilia. These results indicate that GEF1 is the resident Tetrahymena ciliary protein orthologous to PSec7. Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc...
  44. pmc The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium
    Linda Schneider
    Department of Biology, University of Copenhagen, Copenhagen O, Denmark
    J Cell Biol 185:163-76. 2009
    ....
  45. pmc Fluorescent proteins: a cell biologist's user guide
    Erik Lee Snapp
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Cell Biol 19:649-55. 2009
    ..Important FP information, such as oligomer formation or photostability, is often obscure or anecdotal. This brief guide is offered to assist the biologist to exploit FPs in the analysis of cellular processes...
  46. pmc Chaperone-mediated autophagy: selectivity pays off
    Ana Maria Cuervo
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Endocrinol Metab 21:142-50. 2010
    ..Prevention of the age-dependent decline in CMA has major beneficial effects on cellular and organ homeostasis and function, revealing that CMA is an essential component of the anti-aging fight...
  47. pmc Chaperone-mediated autophagy in health and disease
    Maria Kon
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    FEBS Lett 584:1399-404. 2010
    ..Here we review the unique properties of CMA compared to other autophagic pathways and its relevance in health and disease...
  48. pmc Modulatory effects of cAMP and PKC activation on gap junctional intercellular communication among thymic epithelial cells
    Oscar K Nihei
    Laboratory of Cellular Communication, Oswaldo Cruz Institute, The Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    BMC Cell Biol 11:3. 2010
    ..We investigated the effects of the signaling molecules, cyclic AMP (cAMP) and protein-kinase C (PKC), on gap junctional intercellular communication (GJIC) between thymic epithelial cells (TEC)...
  49. pmc Protein homeostasis and aging: The importance of exquisite quality control
    Hiroshi Koga
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Institute for Aging Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Ageing Res Rev 10:205-15. 2011
    ..In this work, we review recent advances on our understanding of the contribution of chaperones and proteolytic systems to the maintenance of cellular homeostasis, the cellular response to stress and ultimately to longevity...
  50. pmc Chaperone-mediated autophagy
    Eloy Bejarano
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Am Thorac Soc 7:29-39. 2010
    ....
  51. pmc Chaperone-mediated autophagy: molecular mechanisms and physiological relevance
    Samantha J Orenstein
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center, Institute for Aging Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Semin Cell Dev Biol 21:719-26. 2010
    ..Changes with age in CMA activity and the contribution of failure of CMA to the phenotype of aging and to the pathogenesis of several age-related pathologies are also described...
  52. pmc Altered lipid content inhibits autophagic vesicular fusion
    Hiroshi Koga
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    FASEB J 24:3052-65. 2010
    ..Changes in the intracellular lipid content (i.e., metabolic disorders) may thus have pronounced effects on the fusion step of macroautophagy and affect the overall activity of this intracellular proteolytic pathway...
  53. pmc Cargo recognition failure is responsible for inefficient autophagy in Huntington's disease
    Marta Martinez-Vicente
    Department of Developmental and Molecular Biology, Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Neurosci 13:567-76. 2010
    ..We propose that inefficient engulfment of cytosolic components by autophagosomes is responsible for their slower turnover, functional decay and accumulation inside HD cells...
  54. pmc Autophagy gone awry in neurodegenerative diseases
    Esther Wong
    Department of Developmental and Molecular Biology, Marion Bessin Liver Research Center and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Neurosci 13:805-11. 2010
    ....
  55. pmc Chaperone-mediated autophagy dysfunction in the pathogenesis of neurodegeneration
    Hiroshi Koga
    Department of Developmental and Molecular Biology, Institute for Aging Research, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA
    Neurobiol Dis 43:29-37. 2011
    ..This article is part of a Special Issue entitled "Autophagy and protein degradation in neurological diseases."..
  56. pmc Reciprocal influence of connexins and apical junction proteins on their expressions and functions
    Mickael Derangeon
    Institut de Physiologie et Biologie Cellulaires, Universite de Poitiers, Poitiers, F 86022, France
    Biochim Biophys Acta 1788:768-78. 2009
    ..Proteins forming gap junction channels (connexins, particularly) and proteins fulfilling cell attachment or forming tight junction strands mutually influence expression and functions of one another...