Jules Stein Eye Institute Core Grant for Vision Research

Summary

Principal Investigator: Wayne L Hubbell
Abstract: DESCRIPTION (provided by applicant): The Vision Research Division of the Jules Stein Eye Institute (JSEI) and affiliated Departments at UCLA consists of 15 vision scientists with NEI funding. The objective of the Core Grant for Vision Research at the JSEI is to provide a critically needed infrastructure to support the research activities of this talented group of scientists whose common goal is to increase basic knowledge of the visual system and its disorders. To achieve this objective, personnel and instrument support facilities are organized according to the following modules: (1) Biochemistry and Molecular Biology Instrumentation;(2) Microscopy and Imaging;(3) Machine Shop and (4) Live imaging and Functional Evaluation. These Modules provide instrumentation commonly used by multiple laboratories and are essential to the success of on-going projects as well as to enable new collaborative efforts. Purchase of state-of-the-art instruments in the modules was made possible by the generosity of the JSEI through various donors as well as recent funds from the American Recovery and Reinvestment Act. Support in the form of the Core grant is requested to maintain these Modules through instrument service contracts, and to provide necessary personnel support to assist and train users and provide routine maintenance.
Funding Period: 1997-03-01 - 2015-02-28
more information: NIH RePORT

Top Publications

  1. pmc Asymmetric phenotype of Axenfeld-Rieger anomaly and aniridia associated with a novel PITX2 mutation
    Simon K Law
    Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Mol Vis 17:1231-8. 2011
  2. pmc Creep behavior of passive bovine extraocular muscle
    Lawrence Yoo
    Department of Ophthalmology, Jules Stein Eye Institute, University of California Los Angeles, 100 Stein Plaza, UCLA, Los Angeles, CA 90095 7002, USA
    J Biomed Biotechnol 2011:526705. 2011
  3. pmc PTEN regulates retinal interneuron morphogenesis and synaptic layer formation
    Kiyo Sakagami
    Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Mol Cell Neurosci 49:171-83. 2012
  4. pmc Functional and morphological analysis of the subretinal injection of retinal pigment epithelium cells
    Maren Engelhardt
    Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Vis Neurosci 29:83-93. 2012
  5. pmc Differential lateral rectus compartmental contraction during ocular counter-rolling
    Robert A Clark
    Department of Ophthalmology, University of California, Los Angeles, CA 90095 7002, USA
    Invest Ophthalmol Vis Sci 53:2887-96. 2012
  6. pmc Retinoid content, visual responses, and ocular morphology are compromised in the retinas of mice lacking the retinol-binding protein receptor, STRA6
    Alberto Ruiz
    Department of Neurobiology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 53:3027-39. 2012
  7. pmc Nonclassical innervation patterns in mammalian extraocular muscles
    Roberta M da Silva Costa
    Department of Ophthalmology, University of California Los Angeles, Jules Stein Eye Institute, 100 Stein Plaza, Los Angeles, CA 90095 7002, USA
    Curr Eye Res 37:761-9. 2012
  8. pmc Pulsed ESR dipolar spectroscopy for distance measurements in immobilized spin labeled proteins in liquid solution
    Zhongyu Yang
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Am Chem Soc 134:9950-2. 2012
  9. pmc Medial rectus recession is as effective as lateral rectus resection in divergence paralysis esotropia
    Zia Chaudhuri
    Jules Stein Eye Institute, University of California, Los Angeles, USA
    Arch Ophthalmol 130:1280-4. 2012
  10. pmc Mapping molecular flexibility of proteins with site-directed spin labeling: a case study of myoglobin
    Carlos J Lopez
    Department of Chemistry and Biochemistry, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095 7008, USA
    Biochemistry 51:6568-83. 2012

Detail Information

Publications152 found, 100 shown here

  1. pmc Asymmetric phenotype of Axenfeld-Rieger anomaly and aniridia associated with a novel PITX2 mutation
    Simon K Law
    Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Mol Vis 17:1231-8. 2011
    ..To evaluate the asymmetry of the anterior segment phenotype between the two eyes of a patient with Axenfeld-Rieger syndrome (ARS)...
  2. pmc Creep behavior of passive bovine extraocular muscle
    Lawrence Yoo
    Department of Ophthalmology, Jules Stein Eye Institute, University of California Los Angeles, 100 Stein Plaza, UCLA, Los Angeles, CA 90095 7002, USA
    J Biomed Biotechnol 2011:526705. 2011
    ..7% following 16.67%/s ramp loading. Measured creep agrees closely with a derived QLV model of EOM relaxation, validating a previous QLV model for characterization of EOM biomechanics...
  3. pmc PTEN regulates retinal interneuron morphogenesis and synaptic layer formation
    Kiyo Sakagami
    Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Mol Cell Neurosci 49:171-83. 2012
    ..Together, these findings demonstrate that suppression of PI3K/Akt signaling by PTEN is crucial for proper neuronal differentiation and normal retinal network formation...
  4. pmc Functional and morphological analysis of the subretinal injection of retinal pigment epithelium cells
    Maren Engelhardt
    Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Vis Neurosci 29:83-93. 2012
    ....
  5. pmc Differential lateral rectus compartmental contraction during ocular counter-rolling
    Robert A Clark
    Department of Ophthalmology, University of California, Los Angeles, CA 90095 7002, USA
    Invest Ophthalmol Vis Sci 53:2887-96. 2012
    ..We used magnetic resonance imaging (MRI) in humans to investigate differential compartmental activity in the rectus EOMs during head tilt, which evokes ocular counter-rolling, a torsional vestibulo-ocular reflex (VOR)...
  6. pmc Retinoid content, visual responses, and ocular morphology are compromised in the retinas of mice lacking the retinol-binding protein receptor, STRA6
    Alberto Ruiz
    Department of Neurobiology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 53:3027-39. 2012
    ..We report generation of a mouse model in which the STRA6 gene has been disrupted functionally to facilitate the study of visual responses, changes in ocular morphology, and retinoid processing under STRA6 protein deficiency...
  7. pmc Nonclassical innervation patterns in mammalian extraocular muscles
    Roberta M da Silva Costa
    Department of Ophthalmology, University of California Los Angeles, Jules Stein Eye Institute, 100 Stein Plaza, Los Angeles, CA 90095 7002, USA
    Curr Eye Res 37:761-9. 2012
    ..Motor nn are classically described as entering the EOMs in their middle thirds. We investigated EOM innervation that does not follow the classic pattern...
  8. pmc Pulsed ESR dipolar spectroscopy for distance measurements in immobilized spin labeled proteins in liquid solution
    Zhongyu Yang
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Am Chem Soc 134:9950-2. 2012
    ..Interspin distances determined using double quantum coherence (DQC) in solution are close to those expected from models, and the narrow distance distribution in each case indicates that the TAM-based spin label is relatively localized...
  9. pmc Medial rectus recession is as effective as lateral rectus resection in divergence paralysis esotropia
    Zia Chaudhuri
    Jules Stein Eye Institute, University of California, Los Angeles, USA
    Arch Ophthalmol 130:1280-4. 2012
    ..To propose medial rectus (MR) recession to be equally as effective as lateral rectus (LR) resection, which has heretofore been the preferred treatment for divergence paralysis esotropia (DPE)...
  10. pmc Mapping molecular flexibility of proteins with site-directed spin labeling: a case study of myoglobin
    Carlos J Lopez
    Department of Chemistry and Biochemistry, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095 7008, USA
    Biochemistry 51:6568-83. 2012
    ....
  11. pmc Function of transected or avulsed rectus muscles following recovery using an anterior orbitotomy approach
    Stacy L Pineles
    Jules Stein Eye Institute and Department of Ophthalmology, University of California, Los Angeles, California, USA
    J AAPOS 16:336-41. 2012
    ..To assess the function of muscles retrieved from a retrobulbar location using an anterior orbitotomy approach and to identify the prognostic factors favoring a good outcome...
  12. pmc Live-cell imaging evidence for the ciliary transport of rod photoreceptor opsin by heterotrimeric kinesin-2
    Deepti Trivedi
    Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles School of Medicine, Los Angeles, California 90095, USA
    J Neurosci 32:10587-93. 2012
    ..These studies therefore provide evidence from live-cell analysis that the conserved heterotrimeric kinesin-2 is required for the normal transport of opsin along the ciliary plasma membrane...
  13. pmc Long-term outcome of medial rectus recession and pulley posterior fixation in esotropia with high AC/A ratio
    Geoffrey Wabulembo
    Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, Los Angeles, CA 90095 7002, USA
    Strabismus 20:115-20. 2012
    ..This study provides a novel examination of the long-term results of MR recession with pulley PF (PPF)...
  14. pmc Functional morphometry of horizontal rectus extraocular muscles during horizontal ocular duction
    Robert A Clark
    Departments of Ophthalmology, David Geffen Medical School, University of California, Los Angeles, California 90095 7002, USA
    Invest Ophthalmol Vis Sci 53:7375-9. 2012
    ..We explored multiple quantitative measures of horizontal rectus extraocular muscle (EOM) morphology to determine the magnetic resonance imaging (MRI) measure best correlating with duction and thus contractility...
  15. pmc Structural states and dynamics of the D-loop in actin
    Zeynep A Oztug Durer
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California, USA
    Biophys J 103:930-9. 2012
    ....
  16. pmc Tryptophan rotamer distribution revealed for the α-helix in tear lipocalin by site-directed tryptophan fluorescence
    Oktay K Gasymov
    Department of Pathology and Jules Stein Eye Institute, University California at Los Angeles, Los Angeles, California 90095, USA
    J Phys Chem B 116:13381-8. 2012
    ..Potentially, this technique is applicable to various proteins including membrane proteins, which are rich in α-helix motif...
  17. pmc Conformation of receptor-bound visual arrestin
    Miyeon Kim
    Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 109:18407-12. 2012
    ..Remarkably, the loops containing residues 139, 157, and 344 appear to have high flexibility in both free arrestin-1 and the P-Rh*complex...
  18. pmc Modulation of mouse rod response decay by rhodopsin kinase and recoverin
    Ching Kang Chen
    Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298 0614, USA
    J Neurosci 32:15998-6006. 2012
    ....
  19. pmc Quantitative analysis of retinal ganglion cell survival with Rbpms immunolabeling in animal models of optic neuropathies
    Jacky M K Kwong
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California 90095, USA
    Invest Ophthalmol Vis Sci 52:9694-702. 2011
    ..To investigate whether a recently described retinal ganglion cell (RGC) marker Rbpms (RNA binding protein with multiple splicing) could be used for RGC quantification in various models of RGC degeneration...
  20. pmc Cell culture model that mimics drusen formation and triggers complement activation associated with age-related macular degeneration
    Lincoln V Johnson
    Center for the Study of Macular Degeneration, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 108:18277-82. 2011
    ..This model system will facilitate the analysis of molecular and cellular aspects of AMD pathogenesis, and the testing of new therapeutic agents for its treatment...
  21. pmc Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e19945. 2011
    ....
  22. pmc SSEA4 is a potential negative marker for the enrichment of human corneal epithelial stem/progenitor cells
    Thuy T Truong
    Cornea and Uveitis Division, Jules Stein Eye Institute, University of California, Los Angeles, California 90095, USA
    Invest Ophthalmol Vis Sci 52:6315-20. 2011
    ..To examine the expression of stage-specific embryonic antigen-4 (SSEA4) in the epithelium of the human ocular surface and characterize SSEA4(+) and SSEA4(-) limbal epithelial cells...
  23. pmc Severe neurologic impairment in mice with targeted disruption of the electrogenic sodium bicarbonate cotransporter NBCe2 (Slc4a5 gene)
    Liyo Kao
    Department of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 286:32563-74. 2011
    ..Our findings are the first demonstration of the fundamental importance of NBCe2 in the biology of the nervous system...
  24. pmc Expansion of the CHN1 strabismus phenotype
    Noriko Miyake
    Departments of Neurology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Invest Ophthalmol Vis Sci 52:6321-8. 2011
    ..This was a study of five family members with distinctive ocular dysmotility patterns that co-segregated with a novel hyperactivating CHN1 mutation...
  25. pmc Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19
    Arturo Ramirez-Miranda
    Cornea and Uveitis Division, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Mol Vis 17:1652-61. 2011
    ..To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium...
  26. pmc Regulation of retinoschisin secretion in Weri-Rb1 cells by the F-actin and microtubule cytoskeleton
    Eiko Kitamura
    Jules Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e20707. 2011
    ....
  27. pmc Harmonin (Ush1c) is required in zebrafish Müller glial cells for photoreceptor synaptic development and function
    Jennifer B Phillips
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403 1254, USA
    Dis Model Mech 4:786-800. 2011
    ..This study shows that visual defects associated with loss of ush1c function in zebrafish can be detected from the onset of vision, and thus could be applicable to early diagnosis for USH1C patients...
  28. pmc Receptor-mediated cellular uptake mechanism that couples to intracellular storage
    Riki Kawaguchi
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, United States
    ACS Chem Biol 6:1041-51. 2011
    ..We have also identified a robust small-molecule-based technique to specifically stimulate cellular vitamin A uptake. This technique has implications in treating human diseases...
  29. pmc ZBED4, a cone and Müller cell protein in human retina, has a different cellular expression in mouse
    Mehrnoosh Saghizadeh
    Jules Stein Eye Institute, UCLA, Los Angeles, CA, USA
    Mol Vis 17:2011-8. 2011
    ..To begin investigating these potential roles of ZBED4, we studied the developmental expression and localization of both the Zbed4 mRNA and protein of mouse retina...
  30. pmc Nonaneurysmal cranial nerve compression as cause of neuropathic strabismus: evidence from high-resolution magnetic resonance imaging
    Tzu Hsun Tsai
    Jules Stein Eye Institute, University of California, Los Angeles, USA
    Am J Ophthalmol 152:1067-1073.e2. 2011
    ..To seek evidence of neurovascular compression of motor cranial nerves (CNs) in otherwise idiopathic neuropathic strabismus using high-resolution magnetic resonance imaging (MRI)...
  31. pmc Retinal disease course in Usher syndrome 1B due to MYO7A mutations
    Samuel G Jacobson
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania, Philadelphia, USA
    Invest Ophthalmol Vis Sci 52:7924-36. 2011
    ....
  32. pmc Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy
    Mark R Fleissner
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 7008, USA
    Proc Natl Acad Sci U S A 108:16241-6. 2011
    ....
  33. pmc Specific interaction of Gαi3 with the Oa1 G-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium
    Alejandra Young
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e24376. 2011
    ..We had previously shown by in-vivo studies that Gαi3-/- and Oa1-/- mice have similar RPE phenotype and decussation patterns. In this paper we analyze the specificity of the Oa1-Gαi interaction...
  34. pmc The many different cellular functions of MYO7A in the retina
    David S Williams
    Department of Neurobiology and Ophthalmology, UCLA School of Medicine, Los Angeles, CA 90024, USA
    Biochem Soc Trans 39:1207-10. 2011
    ..It is likely that the progressive retinal degeneration that occurs in Usher syndrome 1B patients results from a combination of cellular defects in the RPE and photoreceptor cells...
  35. pmc A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data
    Nicolas L Fawzi
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MA 20892 0520, USA
    J Biomol NMR 51:105-14. 2011
    ....
  36. pmc Expanding repertoire in the oculomotor periphery: selective compartmental function in rectus extraocular muscles
    Joseph L Demer
    Department of Ophthalmology, University of California, Los Angeles, Los Angeles, California 90095 7002, USA
    Ann N Y Acad Sci 1233:8-16. 2011
    ..Computational simulation suggests that differential compartmental action in horizontal rectus EOMs could achieve more force than required by vertical fusional vergence...
  37. pmc Mechanical interferometry imaging for creep modeling of the cornea
    Lawrence Yoo
    Department of Ophthalmology, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 52:8420-4. 2011
    ..A novel nanoindentation technique was used to biomechanically characterize each of three main layers of the cornea by using Hertzian viscoelastic formulation of creep, the deformation resulting from sustained-force application...
  38. pmc Does inferior oblique recession cause overcorrections in laterally incomitant small hypertropias due to superior oblique palsy?
    Karen Hendler
    Department of Surgery, Division of Pediatric Ophthalmology, Olive View UCLA Medical Center, 100 Stein Plaza, Los Angeles, CA 90095, USA
    Br J Ophthalmol 97:88-91. 2013
    ..To evaluate the effects of inferior oblique muscle recession (IOR) in cases of laterally incomitant hypertropia <10 prism dioptres (PD) in central gaze thact 2t are clinically consistent with superior oblique palsy (SOP)...
  39. pmc External ophthalmoplegia in human immunodeficiency virus-infected patients receiving antiretroviral therapy
    Stacy L Pineles
    Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, CA, USA
    J AAPOS 16:529-33. 2012
    ..Orbital imaging may help explain the pathogenesis of this abnormality...
  40. pmc A constitutively active Gαi3 protein corrects the abnormal retinal pigment epithelium phenotype of Oa1-/- mice
    Alejandra Young
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 8:e76240. 2013
    ..To test this hypothesis, we have generated transgenic mice that carry the constitutively active Gαi3 (Q204L) protein in the RPE of Oa1-/- mice and are now reporting the effects that the transgene produced on the Oa1-/- RPE phenotype...
  41. pmc Classification of posterior polymorphous corneal dystrophy as a corneal ectatic disorder following confirmation of associated significant corneal steepening
    Anthony J Aldave
    Jules Stein Eye Institute, David Geffen School of Medicine at University of California, Los Angeles
    JAMA Ophthalmol 131:1583-90. 2013
    ....
  42. pmc Conformational selection and adaptation to ligand binding in T4 lysozyme cavity mutants
    Carlos J Lopez
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 7008
    Proc Natl Acad Sci U S A 110:E4306-15. 2013
    ..The results have implications for mechanisms of anesthesia, the response of proteins to hydrostatic pressure, and protein engineering. ..
  43. pmc Frizzled 7 maintains the undifferentiated state of human limbal stem/progenitor cells
    Hua Mei
    Jules Stein Eye Institute, Department of Ophthalmology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
    Stem Cells 32:938-45. 2014
    ..Our finding suggests that Wnt signaling is one of the factors of LSC niche, and Fz7 helps to maintain the undifferentiated state of LSCs...
  44. pmc Receptor interacting protein kinase-mediated necrosis contributes to cone and rod photoreceptor degeneration in the retina lacking interphotoreceptor retinoid-binding protein
    Kota Sato
    Department of Ophthalmology and Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, Electron Microscope Laboratory, Tulane University, New Orleans, Louisiana 70118, Department of Ophthalmology, Georgia Health Sciences University, Augusta, Georgia 30912, and Jules Stein Eye Institute, The University of California, Los Angeles School of Medicine, Los Angeles, California 90095
    J Neurosci 33:17458-68. 2013
    ....
  45. pmc CNTF-mediated protection of photoreceptors requires initial activation of the cytokine receptor gp130 in Müller glial cells
    Kun Do Rhee
    Jules Stein Eye Institute, Department of Ophthalmology, Department of Neurobiology, and Molecular Biology Institute, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 110:E4520-9. 2013
    ..These results elucidate a cellular mechanism for exogenous CNTF-triggered neuroprotection and provide insight into the complex cellular responses induced by CNTF in diseased retinas. ..
  46. pmc Effect of short- and long-range interactions on trp rotamer populations determined by site-directed tryptophan fluorescence of tear lipocalin
    Oktay K Gasymov
    Departments of Pathology and Laboratory Medicine and Ophthalmology and Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 8:e78754. 2013
    ..The SDTF method is a valuable tool to assess long-range interaction energies through rotamer distribution as well as the characterization of low-populated rotameric states of functionally important excited protein states...
  47. pmc Circular dichroism and site-directed spin labeling reveal structural and dynamical features of high-pressure states of myoglobin
    Michael T Lerch
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 110:E4714-22. 2013
    ..1 molten globule of apoMb. Pressure-populated states of both holomyoglobin and apoMb at pH 4.1 have significantly less helical structure, and for the latter, that may correspond to a transient folding intermediate. ..
  48. ncbi Self-association of arrestin family members
    Qiuyan Chen
    Department of Pharmacology, Vanderbilt University, 2200 Pierce Avenue, Nashville, TN, 37232, USA
    Handb Exp Pharmacol 219:205-23. 2014
    ..The physiological role of the oligomerization of arrestin-1 and both nonvisual arrestins might be quite different, and in each case it remains to be definitively elucidated. ..
  49. pmc Biomechanics of superior oblique Z-tenotomy
    Andrew Shin
    Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles Department of Mechanical and Aerospace Engineering, University of California, Los Angeles
    J AAPOS 17:612-7. 2013
    ..To clarify the clinical effect, we compared the biomechanics of Z-tenotomy on the superior oblique tendon, superior rectus tendon, and isotropic latex material...
  50. ncbi Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1α in human corneal limbal epithelial cells
    Negin Ashki
    Department of Ophthalmology, Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California
    Invest Ophthalmol Vis Sci 55:1637-46. 2014
    ..The role of ONOO(-) in upregulating multiple angiogenic factors in cultured human corneal limbal epithelial (HCLE) cells was investigated...
  51. pmc Probing tertiary structure of proteins using single Trp mutations with circular dichroism at low temperature
    Oktay K Gasymov
    Departments of Pathology and Ophthalmology and Jules Stein Eye Institute, University California at Los Angeles, California 90095, United States
    J Phys Chem B 118:986-95. 2014
    ..The Trp CD spectral classification of Strickland was modified to facilitate the recognition of pseudo peaks. Near-UV CD spectra harbor abundant information about the conformation of proteins that site directed Trp CD can report. ..
  52. pmc Bisretinoid-mediated complement activation on retinal pigment epithelial cells is dependent on complement factor H haplotype
    Roxana A Radu
    From the Stein Eye Institute, Department of Ophthalmology
    J Biol Chem 289:9113-20. 2014
    ..Cells with an intact complement negative regulatory system are protected from complement attack, whereas cells with reduced CFH synthesis because of the Y402H and I62V substitutions are vulnerable to disease. ..
  53. pmc Muscle path length in horizontal strabismus
    Ronen Rabinowitz
    Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles
    J AAPOS 18:4-9. 2014
    ..The purpose of this study was to investigate whether horizontal rectus muscle paths have abnormal lengths in subjects with intermittent or alternating strabismus...
  54. pmc Mapping protein conformational heterogeneity under pressure with site-directed spin labeling and double electron-electron resonance
    Michael T Lerch
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 111:E1201-10. 2014
    ..Comparison of the distance distributions for pressure- and pH-populated molten globules shows them to be remarkably similar despite a lower helical content in the latter. ..
  55. pmc Atomic force microscopy determination of Young׳s modulus of bovine extra-ocular tendon fiber bundles
    Lawrence Yoo
    Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095 7002, USA
    J Biomech 47:1899-903. 2014
    ..This uniformity will simplify overall modeling. ..
  56. pmc A three-dimensional culture method to expand limbal stem/progenitor cells
    Hua Mei
    Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles, California
    Tissue Eng Part C Methods 20:393-400. 2014
    ..This alternative method permits culturing of LSCs without the risk of feeder cell contamination. ..
  57. pmc A simple model-free method for direct assessment of fluorescent ligand binding by linear spectral summation
    Oktay K Gasymov
    Departments of Ophthalmology, Pathology and Laboratory Medicine, Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, CA, 90095, USA
    J Fluoresc 24:231-8. 2014
    ..Ligands tagged with environmentally sensitive fluorescent dyes, e.g., dansyl chromophore, are particularly suitable for this method...
  58. pmc Dnmt1-dependent DNA methylation is essential for photoreceptor terminal differentiation and retinal neuron survival
    K D Rhee
    Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA
    Cell Death Dis 3:e427. 2012
    ..These results indicate that Dnmt1-dependent DNA methylation is critical for expansion of the retinal progenitor pool, as well as for maturation and survival of postmitotic neurons...
  59. pmc Independent passive mechanical behavior of bovine extraocular muscle compartments
    Andrew Shin
    Department of Ophthalmology, Jules Stein Eye Institute, Los Angeles, California 90095 7002, USA
    Invest Ophthalmol Vis Sci 53:8414-23. 2012
    ..We sought evidence of potential independent action by examining passive mechanical coupling between EOM compartments...
  60. pmc Retinal gene therapy with a large MYO7A cDNA using adeno-associated virus
    V S Lopes
    Departments of Ophthalmology and Neurobiology, Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Gene Ther 20:824-33. 2013
    ..Our results show that MYO7A therapy with AAV2 or AAV5 single vectors is efficacious; however, the dual AAV2 approach proved to be less effective. ..
  61. pmc Functional magnetic resonance imaging of horizontal rectus muscles in esotropia
    Kirsta Schoeff
    Jules Stein Eye Institute, Department of Ophthalmology, University of California, Los Angeles, CA 90095 7002, USA
    J AAPOS 17:16-21. 2013
    ..We used magnetic resonance imaging to measure horizontal rectus muscle size and contractility to determine whether muscle structure is a factor in human concomitant esotropia...
  62. pmc A novel fluorescent lipid probe for dry eye: retrieval by tear lipocalin in humans
    Po Ting Yeh
    Departments of Ophthalmology and Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California Los Angeles, Jules Stein Eye Institute, Los Angeles, California 90095, USA
    Invest Ophthalmol Vis Sci 54:1398-410. 2013
    ..A fluorescent probe was used to identify mucin-depleted areas on the ocular surface and to test the hypothesis that tear lipocalin retrieves lipids from the eyes of normal and dry eye subjects...
  63. pmc Dynamics of the rhomboid-like protein RHBDD2 expression in mouse retina and involvement of its human ortholog in retinitis pigmentosa
    Novruz B Ahmedli
    Jules Stein Eye Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 288:9742-54. 2013
    ..Our findings suggest that the RHBDD2 protein plays important roles in the development and normal function of the retina...
  64. pmc Long-term follow-up of strabismus surgery for patients with ocular myasthenia gravis
    Jason H Peragallo
    Department of Ophthalmology, Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, CA 90095, USA
    J Neuroophthalmol 33:40-4. 2013
    ..Patients with ocular myasthenia gravis (OMG) may develop strabismus and diplopia. We completed a retrospective observational case series to evaluate long-term surgical outcomes in patients with OMG...
  65. pmc Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme
    Luca Garbuio
    Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland
    J Phys Chem B 117:3145-53. 2013
    ..A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity...
  66. pmc Determination of poisson ratio of bovine extraocular muscle by computed X-ray tomography
    Hansang Kim
    Department of Mechanical and Automotive Engineering, Gachon University, Seongnam si, Gyeonggi do 461 701, Republic of Korea
    Biomed Res Int 2013:197479. 2013
    ..The mean PR from discretized 3D models for every microscopic element in 14 EOM specimens averaged 0.457 ± 0.004 (SD). The measured PR of bovine EOM is thus near the limit of incompressibility...
  67. pmc Exclusion of pathogenic promoter region variants and identification of novel nonsense mutations in the zinc finger E-box binding homeobox 1 gene in posterior polymorphous corneal dystrophy
    Pejman Bakhtiari
    The Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 7003, USA
    Mol Vis 19:575-80. 2013
    ....
  68. pmc Preferential biological processes in the human limbus by differential gene profiling
    Martin N Nakatsu
    Cornea Division, Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 8:e61833. 2013
    ..These results could aid in a better understanding of the regulatory elements in the LSC microenvironment...
  69. pmc Structure and dynamics of an imidazoline nitroxide side chain with strongly hindered internal motion in proteins
    Dora Toledo Warshaviak
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    J Magn Reson 232:53-61. 2013
    ....
  70. pmc Fundus autofluorescence in the Abca4(-/-) mouse model of Stargardt disease--correlation with accumulation of A2E, retinal function, and histology
    Peter Charbel Issa
    Oxford Eye Hospital and Nuffield Laboratory of Ophthalmology, University of Oxford, Oxford, United Kingdom
    Invest Ophthalmol Vis Sci 54:5602-12. 2013
    ..To investigate fundus autofluorescence (AF) characteristics in the Abca4(-/-) mouse, an animal model for AMD and Stargardt disease, and to correlate findings with functional, structural, and biochemical assessments...
  71. pmc EPR relaxation-enhancement-based distance measurements on orthogonally spin-labeled T4-lysozyme
    Sahand Razzaghi
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Strasse 10, 8093 Zurich Switzerland
    Chembiochem 14:1883-90. 2013
    ..Studies of conformational changes and of bio-macromolecule association-dissociation are proposed as possible application area of the relaxation-enhancement-based distance measurements. ..
  72. pmc Technological advances in site-directed spin labeling of proteins
    Wayne L Hubbell
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, United States Electronic address
    Curr Opin Struct Biol 23:725-33. 2013
    ....
  73. pmc Rapid degeneration of rod photoreceptors expressing self-association-deficient arrestin-1 mutant
    Xiufeng Song
    Vanderbilt University, Nashville, TN 37232, United States
    Cell Signal 25:2613-24. 2013
    ..Thus, arrestin-1 self-association likely serves to keep low concentration of the toxic monomer. The reduction of the concentration of harmful monomer is an earlier unappreciated biological function of protein oligomerization. ..
  74. pmc Presence of native limbal stromal cells increases the expansion efficiency of limbal stem/progenitor cells in culture
    Sheyla González
    Cornea Division, Jules Stein Eye Institute, University of California, Los Angeles, 100 Stein Plaza, Los Angeles, CA 90095, USA Electronic address
    Exp Eye Res 116:169-76. 2013
    ..Our finding suggests that limbal stromal cells and an intact cell-cell contact help to maintain LSCs in an undifferentiated state in vitro during expansion. ..
  75. pmc Identification of the 11-cis-specific retinyl-ester synthase in retinal Müller cells as multifunctional O-acyltransferase (MFAT)
    Joanna J Kaylor
    Jules Stein Eye Institute and
    Proc Natl Acad Sci U S A 111:7302-7. 2014
    ..MFAT is therefore an excellent candidate for the retinyl-ester synthase that cooperates with DES1 to drive synthesis of 11-cis-retinoids by mass action. ..
  76. ncbi Bipolar assembly of caveolae in retinal pigment epithelium
    Rosalia C Mora
    M Dyson Vision Research Institute, Weill Medical College of Cornell University, 1300 York Ave, New York, NY 10021, USA
    Am J Physiol Cell Physiol 290:C832-43. 2006
    ..The bipolar localization of plasmalemmal caveolae in RPE cells may reflect specialized roles in signaling and trafficking important for visual function...
  77. pmc Oligomeric state of lipocalin-1 (LCN1) by multiangle laser light scattering and fluorescence anisotropy decay
    Oktay K Gasymov
    Department of Pathology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Biochim Biophys Acta 1774:1307-15. 2007
    ..These findings are important as the dynamic processes in the tear film are limited by diffusional, translational as well as rotational, properties of the protein...
  78. pmc Evidence for internal and external binding sites on human tear lipocalin
    Oktay K Gasymov
    Department of Pathology, UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, Rm B269, Los Angeles, CA 90095, USA
    Arch Biochem Biophys 468:15-21. 2007
    ..Time-resolved fluorescence is a promising tool to segregate multiple binding sites of proteins...
  79. pmc Characterization of fluorescence of ANS-tear lipocalin complex: evidence for multiple-binding modes
    Oktay K Gasymov
    Department of Pathology, UCLA School of Medicine, Jules Stein Eye Institute, Los Angeles, CA, USA
    Photochem Photobiol 83:1405-14. 2007
    ..The two lifetime components are not derived from solvent relaxation, but rather may represent different binding modes...
  80. pmc Site-directed circular dichroism of proteins: 1Lb bands of Trp resolve position-specific features in tear lipocalin
    Oktay K Gasymov
    Departments of Pathology and Ophthalmology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Anal Biochem 374:386-95. 2008
    ..Combined with Trp mutagenesis, this method, which we call site-directed circular dichroism, is broadly applicable to various proteins to obtain the position-specific data...
  81. ncbi Somatic ablation of the Lrat gene in the mouse retinal pigment epithelium drastically reduces its retinoid storage
    Alberto Ruiz
    Jules Stein Eye Institute, University of California, Los Angeles 90095 7002, USA
    Invest Ophthalmol Vis Sci 48:5377-87. 2007
    ..To evaluate the effects on the synthesis of retinyl esters and on the expression of other proteins involved in the continuation of the visual cycle...
  82. pmc A novel form of transducin-dependent retinal degeneration: accelerated retinal degeneration in the absence of rod transducin
    Elliott Brill
    Molecular Cardiology Research Institute, Tufts New England Medical Center, Boston, Massachusetts, USA
    Invest Ophthalmol Vis Sci 48:5445-53. 2007
    ..This study examined whether three disease-associated rhodopsin substitutions Pro347Ser, Lys296Glu, and the triple mutant Val20Gly, Pro23His, Pro27Leu (VPP) caused degeneration by persistent transducin-mediated signaling activity...
  83. pmc Magnetic resonance imaging of innervational and extraocular muscle abnormalities in Duane-radial ray syndrome
    Joseph L Demer
    Department of Ophthalmology, Jules Stein Eye Institute, University of California, Los Angeles, CA 90095 7002, USA
    Invest Ophthalmol Vis Sci 48:5505-11. 2007
    ..The authors used magnetic resonance imaging (MRI) to study extraocular muscles (EOMs) and nerves in Duane-radial ray (Okihiro) syndrome (DRRS) caused by mutations in the transcription factor SALL4...
  84. pmc Ligand binding site of tear lipocalin: contribution of a trigonal cluster of charged residues probed by 8-anilino-1-naphthalenesulfonic acid
    Oktay K Gasymov
    Department of Pathology, Jules Stein Eye Institute, UCLA School of Medicine, 100 Stein Plaza, Los Angeles, California 90095, USA
    Biochemistry 47:1414-24. 2008
    ..At the aqueous-lipid interface, fatty acids whose carboxyl groups are positioned toward the aqueous phase are available for interaction with TL that could augment stability of the tear film...
  85. pmc Magnetic resonance imaging of the endophenotype of a novel familial Möbius-like syndrome
    Sean Dumars
    Jules Stein Eye Institute Department of Ophthalmology, Los Angeles, California, USA
    J AAPOS 12:381-9. 2008
    ..We used high-resolution magnetic resonance imaging (MRI) and genetic analysis to examine a family with features of Möbius syndrome...
  86. pmc Inflection in inactive lateral rectus muscle: evidence suggesting focal mechanical effects of connective tissues
    Joseph L Demer
    Department of Ophthalmology, Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, California 90095 7002, USA
    Invest Ophthalmol Vis Sci 49:4858-64. 2008
    ..Paths of inactive lateral rectus (LR) muscles were studied to investigate putative roles of orbital fat and intrinsic muscle stiffness suggested to be alternatives to connective tissue pulleys as determinants of pulling direction...
  87. pmc Magnetic resonance imaging of the functional anatomy of the inferior rectus muscle in superior oblique muscle palsy
    Li Jiang
    Department of Ophthalmology, David Geffen Medical School, University of California, Los Angeles, Los Angeles, California, USA
    Ophthalmology 115:2079-86. 2008
    ..Magnetic resonance imaging (MRI) was used to investigate if any size or contractility changes in the inferior rectus (IR) muscle may contribute...
  88. pmc Functional anatomy of the extraocular muscles during vergence
    Joseph L Demer
    Jules Stein Eye Institute, David Geffen Medical School at University of California, Los Angeles, CA, USA
    Prog Brain Res 171:21-8. 2008
    ..Thus, extraretinal information about eye position would appear necessary to interpret binocular correspondence, and to avoid diplopia...
  89. pmc Posterior inflection of weakened lateral rectus path: connective tissue factors reduce response to lateral rectus recession
    Robert A Clark
    Department of Ophthalmology, Jules Stein Eye Institute, UCLA, Los Angeles, California, USA
    Am J Ophthalmol 147:127-133.e2. 2009
    ..To determine why lateral rectus (LR) muscle recession has a variable effect on binocular alignment using magnetic resonance imaging (MRI)...
  90. pmc High-resolution magnetic resonance imaging demonstrates reduced inferior oblique muscle size in isolated inferior oblique palsy
    Noa Ela-Dalman
    Department of Ophthalmology, David Geffen Medical School at University of California Los Angeles, Los Angeles, California, USA
    J AAPOS 12:602-7. 2008
    ..Because denervated extraocular muscles atrophy, we used high-resolution magnetic resonance imaging (MRI) to assess inferior oblique muscle size in patients with clinically suspected inferior oblique muscle palsy...
  91. pmc "Heavy Eye" syndrome in the absence of high myopia: A connective tissue degeneration in elderly strabismic patients
    Tina Rutar
    Department of Ophthalmology, University of California, Los Angeles, Los Angeles, California, USA
    J AAPOS 13:36-44. 2009
    ..We investigated whether this degeneration might also cause strabismus in nonmyopic elderly patients...
  92. pmc Night blindness and the mechanism of constitutive signaling of mutant G90D rhodopsin
    Alexander M Dizhoor
    Hafter Research Laboratories, Pennsylvania College of Optometry, Salus University, Elkins Park, Pennsylvania 19027, USA
    J Neurosci 28:11662-72. 2008
    ..Our experiments provide the first indication that a mutant form of the rhodopsin molecule bound to its 11-cis-chromophore can stimulate the visual cascade spontaneously at a rate large enough to produce visual dysfunction...
  93. pmc Decreased postoperative drift in intermittent exotropia associated with A and V patterns
    Stacy L Pineles
    Jules Stein Eye Institute and Department of Ophthalmology, University of California, Los Angeles, CA 90095 7002, USA
    J AAPOS 13:127-31. 2009
    ..Few data exist concerning postoperative drift in patients with intermittent exotropia who have coexistent A or V patterns. In addition, the impacts of pattern collapse and surgical method on postoperative drift have not been well addressed...
  94. pmc Constitutive excitation by Gly90Asp rhodopsin rescues rods from degeneration caused by elevated production of cGMP in the dark
    Michael L Woodruff
    Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
    J Neurosci 27:8805-15. 2007
    ..These experiments indicate that mutations that lead to increases in cGMP and Ca(2+) can trigger photoreceptor degeneration but that constitutive activation of the transduction cascade in these animals can greatly enhance cell survival...
  95. pmc Neurotrophins enhance retinal pigment epithelial cell survival through neuroprotectin D1 signaling
    Pranab K Mukherjee
    Neuroscience Center and Department of Ophthalmology, Louisiana State University Health Sciences Center School of Medicine, New Orleans, LA 70112, USA
    Proc Natl Acad Sci U S A 104:13152-7. 2007
    ..Taken together, these findings imply NPD1 may elicit autocrine actions on RPE cells and paracrine bioactivity in cells located in the proximity of the interphotoreceptor matrix...
  96. pmc Magnetic resonance imaging of the effects of horizontal rectus extraocular muscle surgery on pulley and globe positions and stability
    Robert A Clark
    Department of Ophthalmology, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 47:188-94. 2006
    ..Magnetic resonance imaging (MRI) was used to determine the effect of recessions and resections on horizontal extraocular muscle (EOM) paths and globe position...
  97. pmc Current concepts of mechanical and neural factors in ocular motility
    Joseph L Demer
    Jules Stein Eye Institute, Department of Ophthalmology, David Geffen Medical School at University of California, Los Angeles, California 90095 7002, USA
    Curr Opin Neurol 19:4-13. 2006
    ..This review considers current concepts regarding aspects of ocular motility that are mechanically determined versus those that are specified explicitly as innervation...
  98. ncbi Inferior rectus muscle transection: a cause of diplopia after non-penetrating orbital trauma
    Chee Chew Yip
    Orbital and Ophthalmic Plastic Surgery Division, Jules Stein Eye Institute, David Geffen Medical School at UCLA, Los Angeles, CA, USA
    Graefes Arch Clin Exp Ophthalmol 244:1698-700. 2006
    ..Vertical diplopia after blow out fracture repair is not uncommon; we report an unusual case of inferior rectus muscle (IR) transection presenting as a persistent infra-duction deficit after uncomplicated blow out fracture repair...
  99. pmc High-resolution magnetic resonance imaging demonstrates abnormalities of motor nerves and extraocular muscles in patients with neuropathic strabismus
    Joseph L Demer
    Department of Ophthalmology, Jules Stein Dye Institute, University of California, Los Angeles, California 90095 7002, and Department of Medicine, Children s Hospital Boston, MA, USA
    J AAPOS 10:135-42. 2006
    ....
  100. ncbi Glucose utilization by the retinal pigment epithelium: evidence for rapid uptake and storage in glycogen, followed by glycogen utilization
    Preenie deS Senanayake
    Department of Ophthalmic Research, The Cole Eye Institute, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Exp Eye Res 83:235-46. 2006
    ..These studies are the first to demonstrate glucose, glycogen and maltodextrin metabolism by RPE cells, and their detection and quantitation by FACE...