Genomes and Genes
Intrarenal Angiotensin II generation during Angiotensin II-induced hypertension
Principal Investigator: ROMER ANDRES GONZALEZ-VILLALOBOS
Abstract: The long-term goals of this project are to establish the importance and underlying mechanisms mediating the effects of intrarenal ACE-derived Ang II generation on the development of hypertension and renal injury while facilitating the applicant[unreadable]s transition to an independent career in the field of kidney disease. The kidneys posses all the components of the renin-angiotensin system and therefore are capable of synthesizing Ang II. Because the kidneys play a preponderant role in fluid homeostasis and blood pressure regulation, it is likely that an augmented intrarenal Ang II generation is of cardinal importance for the development of hypertension and renal damage but this has not been determined. Previous studies by the applicant demonstrate that the kidneys from Ang II-infused mice display an augmented angiotensinogen expression and persistence of renin and angiotensin-converting enzyme (ACE) activities that suggest the presence of sustained intrarenal Ang II generation. Recent reports have provided evidence for functional interactions between Ang II and microRNAs. Because a single microRNA can regulate the expression of multiple genes, it is possible that in generating complex phenotypes like hypertension and kidney damage intrarenal Ang II modulates one or several microRNAs. In particular, microRNA 21 (miR-21) has gained recognition in cardiovascular disease because of its involvement in cardiac hypertrophy, apoptosis, inflammation, and as mediator of some Ang II actions, but its role in kidney disease has not been determined. Therefore, the HYPOTHESIS to be tested is that during Ang II-induced hypertension, intrarenal ACE-derived Ang II formation is required in order to augment Ang II levels in the kidney that in turn increase sodium and water retention, increase miR-21 expression, and lead to the progressive development of high blood pressure and renal injury. Experiments will be conducted in the department of Physiology of Tulane University in collaboration with Cedars-Sinai Center from Los Angeles, CA. Tissue-specific ACE knockout mice will be used in order to address this hypothesis and the following SPECIFIC AIMS are proposed: 1) To demonstrate that mice with impaired intrarenal Ang II formation, as a consequence of the absence of ACE in the kidneys, develop lesser increases in intrarenal Ang II content, sodium retention, blood pressure levels and kidney injury during chronic Ang II infusions when compared to wild-type controls. 2) To demonstrate that mice with ACE expression only in the kidneys develop increases in intrarenal Ang II content and sodium retention along with increased blood pressure levels and kidney injury during chronic infusions of the ACE substrate Ang I. 3) To demonstrate that miR-21 is upregulated in the mouse kidney as a consequence of an augmented intrarenal Ang II generation during Ang II-induced hypertension and that this is an important mechanism for the development of hypertension and renal injury.
Funding Period: 2011-08-01 - 2014-05-31
more information: NIH RePORT
- A modern understanding of the traditional and nontraditional biological functions of angiotensin-converting enzymeKenneth E Bernstein
Cedars Sinai Medical Center, 8700 Beverly Boulevard, Davis 2021, Los Angeles, CA 90048, USA
Pharmacol Rev 65:1-46. 2013..In turn, this knowledge should allow clinicians to envision new therapies for diseases not currently treated with ACE inhibitors...
- The absence of intrarenal ACE protects against hypertensionRomer A Gonzalez-Villalobos
Department of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, California 90048, USA
J Clin Invest 123:2011-23. 2013..In particular, renal ACE activity is required to increase local Ang II, to stimulate sodium transport in loop of Henle and the distal nephron, and to induce hypertension...
- Rediscovering ACE: novel insights into the many roles of the angiotensin-converting enzymeRomer A Gonzalez-Villalobos
Department of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA, 90048, USA
J Mol Med (Berl) 91:1143-54. 2013..It is these features which explain why ACE makes important contributions to many different physiological processes including renal development, blood pressure control, inflammation, and immunity. ..
- Renal angiotensin-converting enzyme and blood pressure controlKenneth E Bernstein
Departments of Biomedical Sciences and Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, CA, USA
Curr Opin Nephrol Hypertens 23:106-12. 2014..This review presents novel findings regarding the renal angiotensin-converting enzyme (ACE) and its role in blood pressure (BP) control...
- ACE overexpression in myelomonocytic cells: effect on a mouse model of Alzheimer's diseaseMaya Koronyo-Hamaoui
Department of Neurosurgery and the Maxine Dunitz Neurosurgical Research Institute, Cedars Sinai Medical Center, Los Angeles, CA, 90048, USA
Curr Hypertens Rep 16:444. 2014....
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