BIOLOGY AND CONTROL OF LYME DISEASE BORRELIA

Summary

Principal Investigator: A G Barbour
Abstract: DESCRIPTION (Adapted from Applicant's Abstract): The proposed collaborative project is based collectively on investigations of the pathogenesis and epidemiology of Lyme disease, the ecology, vector biology, and population genetics of vector-borne diseases, the molecular biology of Borrelia burgdorferi, and vaccine development. The emphases of the proposed studies are the evolutionary biology of B. burgdorferi and new strategies for Lyme disease prevention. The long term goals are the following: (1) To significantly reduce the risk of Lyme disease among humans and domestic animals by vaccine intervention in the natural maintenance cycle of B. burgdorferi in a highly endemic area. (2) To study the evolution of B. burgdorferi as an infectious agent through controlled intervention studies in a highly endemic area. (3)To provide for a vaccine that is highly effective and that can be expeditiously, safely, and inexpensively administered in the field. The hypotheses for the project are the following: (A) A high prevalence of anti-OspA antibodies among Peromyscus leucopus and/or other mammalian reservoirs in the field will reduce the transmission rate between reservoir hosts and vector ticks, thereby reducing the prevalence of B. burgdorferi infection in ticks. (B) Immunization of reservoirs in the field will not alter the population structure of B. burgdorferi at the vaccine field sites in comparison to the control sites. (C) Transmission-blocking immunity among P. leucopus and/or other mammalian reservoirs can be achieved by oral as well as by parenteral immunization with a single immunogen or combined immunogens. The specific aims are the following: (1) Field studies of the effect of immunization of P. leucopus on the rate of transmission of B. burgdorferi between reservoir host and vector ticks. (2) Assessment of the effects of vaccine intervention in the field on the population structure of B. burgdorferi in ticks and in vertebrate reservoirs. (3) Further development of single-vaccination and orally-delivered field vaccines and evaluation of second, supplementary antigens. The proposed studies may provide insights as molecular, organismal, and population levels about B. burgdorferi, its transmission, and reservoir host immunize responses. The results may also have relevance for the control of other vector-bone zoonotic diseases and perhaps for development of novel methods for vaccine delivery.
Funding Period: 1994-09-30 - 2006-04-30
more information: NIH RePORT

Top Publications

  1. ncbi Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target
    Michael Becker
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 280:17363-70. 2005
  2. pmc Third Borrelia species in white-footed mice
    Jonas Bunikis
    Emerg Infect Dis 11:1150-1. 2005
  3. ncbi A reverse transcriptase-polymerase chain reaction assay of Borrelia burgdorferi 16S rRNA for highly sensitive quantification of pathogen load in a vector
    Katharina Ornstein
    Clinical and Experimental Infectious Medicine Section, Department of Clinical Sciences, Lund University, Lund, Sweden
    Vector Borne Zoonotic Dis 6:103-12. 2006
  4. pmc Epidemic spread of Lyme borreliosis, northeastern United States
    Klára Hanincová
    Yale University, New Haven, Connecticut, USA
    Emerg Infect Dis 12:604-11. 2006
  5. pmc Migratory passerine birds as reservoirs of Lyme borreliosis in Europe
    Pär Comstedt
    Department of Microbiology, Umea University, Umea, Sweden
    Emerg Infect Dis 12:1087-95. 2006
  6. pmc Niche partitioning of Borrelia burgdorferi and Borrelia miyamotoi in the same tick vector and mammalian reservoir species
    Alan G Barbour
    Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California 92697 4028, USA
    Am J Trop Med Hyg 81:1120-31. 2009
  7. pmc Dissemination of spotted fever rickettsia agents in Europe by migrating birds
    Karin Elfving
    Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden
    PLoS ONE 5:e8572. 2010

Scientific Experts

  • J Bunikis
  • Michael Becker
  • A G Barbour
  • Katharina Ornstein
  • Karin Elfving
  • Bjorn Olsen
  • Sven Bergstrom
  • Hans Mejlon
  • Klára Hanincová
  • Pär Comstedt
  • Ake Lundkvist
  • Kenneth Nilsson
  • Jonas Waldenstrom
  • Anders Sjostedt
  • Maria Diuk-Wasser
  • Ulf Garpmo
  • Klaus Kurtenbach
  • Lisette Marjavaara
  • Durland Fish
  • Brandon Brei

Detail Information

Publications7

  1. ncbi Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target
    Michael Becker
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 280:17363-70. 2005
    ..OspB structure, stability, and possible mechanisms of killing by H6831 and other bactericidal Fabs are discussed in light of the structural data...
  2. pmc Third Borrelia species in white-footed mice
    Jonas Bunikis
    Emerg Infect Dis 11:1150-1. 2005
  3. ncbi A reverse transcriptase-polymerase chain reaction assay of Borrelia burgdorferi 16S rRNA for highly sensitive quantification of pathogen load in a vector
    Katharina Ornstein
    Clinical and Experimental Infectious Medicine Section, Department of Clinical Sciences, Lund University, Lund, Sweden
    Vector Borne Zoonotic Dis 6:103-12. 2006
    ..In contrast, there were either no or <or=7 spirochetes in ticks that had fed on OspA-immunized mice...
  4. pmc Epidemic spread of Lyme borreliosis, northeastern United States
    Klára Hanincová
    Yale University, New Haven, Connecticut, USA
    Emerg Infect Dis 12:604-11. 2006
    ..burgdorferi is a generalist microparasite and conclude that efficient cross-species transmission of B. burgdorferi is a key feature that has allowed the rapid spread of Lyme borreliosis across the northeastern United States...
  5. pmc Migratory passerine birds as reservoirs of Lyme borreliosis in Europe
    Pär Comstedt
    Department of Microbiology, Umea University, Umea, Sweden
    Emerg Infect Dis 12:1087-95. 2006
    ..Migratory passerine birds host epidemiologically important vector ticks and Borrelia species and vary in effectiveness as reservoirs on the basis of their feeding behavior...
  6. pmc Niche partitioning of Borrelia burgdorferi and Borrelia miyamotoi in the same tick vector and mammalian reservoir species
    Alan G Barbour
    Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California 92697 4028, USA
    Am J Trop Med Hyg 81:1120-31. 2009
    ..miyamotoi averaged approximately 0.20 and approximately 0.02, respectively. Co-infections of P. leucopus or I. scapularis with both B. burgdorferi and B. miyamotoi were neither more nor less common than random expectations...
  7. pmc Dissemination of spotted fever rickettsia agents in Europe by migrating birds
    Karin Elfving
    Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden
    PLoS ONE 5:e8572. 2010
    ..In conclusion, migratory passerine birds host epidemiologically important vector ticks and Rickettsia species and contribute to the geographic distribution of spotted fever rickettsial agents and their diseases...