TRANSCRIPTIONAL CONTROL OF TH2 CELL DIFFERENTIATION

Summary

Principal Investigator: Wei ping Zeng
Abstract: DESCRIPTION (Adapted from the Investigator's abstract): TCR stimulation of naive CD4 T-cells in the presence of IL-4 leads to the differentiation of type 2 T helper (Th2) cells. An experimental system will be developed to determine the roles of the transcription factor GATA-3 in the establishment and maintenance of Th2 cells. The investigator aims to identify additional transcription factors that are also critical to the differentiation process. In addition, DNA-protein interactions involving GATA-3 and Stat6 will be studied. The ultimate goal of the research project is to provide a clear understanding of the molecular pathways that lead to the differentiation and functional activation of Th2 cells. It is proposed that knowledge gained from these studies will advance understanding and improve treatment of immunological diseases such as allergy and infection where Th2 responses are critical.
Funding Period: 2000-05-01 - 2006-04-30
more information: NIH RePORT

Top Publications

  1. pmc Differential regulation of osteoblast activity by Th cell subsets mediated by parathyroid hormone and IFN-gamma
    Nathan Young
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 175:8287-95. 2005
  2. ncbi Responsiveness of naive CD4 T cells to polarizing cytokine determines the ratio of Th1 and Th2 cell differentiation
    Natallia Mikhalkevich
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 176:1553-60. 2006
  3. ncbi Temporal and spatial changes of histone 3 K4 dimethylation at the IFN-gamma gene during Th1 and Th2 cell differentiation
    Heli K Hamalainen-Laanaya
    David Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 179:6410-5. 2007
  4. ncbi Interaction between GATA-3 and the transcriptional coregulator Pias1 is important for the regulation of Th2 immune responses
    Xinyan Zhao
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Sciences, Department of Microbiology, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    J Immunol 179:8297-304. 2007

Detail Information

Publications4

  1. pmc Differential regulation of osteoblast activity by Th cell subsets mediated by parathyroid hormone and IFN-gamma
    Nathan Young
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 175:8287-95. 2005
    ..Our study therefore identified new regulatory events in bone remodeling under inflammatory conditions...
  2. ncbi Responsiveness of naive CD4 T cells to polarizing cytokine determines the ratio of Th1 and Th2 cell differentiation
    Natallia Mikhalkevich
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 176:1553-60. 2006
    ..Thus, the IL-4Ralpha level on naive CD4 T cells is genetically controlled by Hlx and determines the ratio of Th1 and Th2 cell differentiation...
  3. ncbi Temporal and spatial changes of histone 3 K4 dimethylation at the IFN-gamma gene during Th1 and Th2 cell differentiation
    Heli K Hamalainen-Laanaya
    David Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Sciences, Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 179:6410-5. 2007
    ....
  4. ncbi Interaction between GATA-3 and the transcriptional coregulator Pias1 is important for the regulation of Th2 immune responses
    Xinyan Zhao
    David H Smith Center for Vaccine Biology and Immunology, Aab Institute for Biomedical Sciences, Department of Microbiology, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    J Immunol 179:8297-304. 2007
    ..In contrast, IL-5 promoter was modestly enhanced by Pias1 and no effect was observed on IL-4 promoter. Thus, both promoter activation and additional mechanisms are responsible for regulation by Pias1...