LC-MS/MS for Biomedical Research

Summary

Principal Investigator: WILLIAM BRYSON CAMPBELL
Abstract: Funds are requested to purchase a liquid chromatograph - triple quadrupole - mass spectrometer (LC-TQ MS) Waters Quattro micro API Tandem Quadrupole System) for use as a shared instrument by researchers of the Medical College of Wisconsin. The College has 950 faculty and NIH funding but has no shared LC-MS/MS instrumentation. This instrument is critical for future research. During the past 11 years, the Mass Spectrometry Shared Facility of the Department of Pharmacology and Toxicology has provided mass spectrometry expertise and instrumentation to the faculty of the College. The Facility has established protocols for handling samples from multiple users and dealing with samples with different chemical characteristics. It has a Hewlett - Packard gas chromatograph - single quadrupole mass spectrometer (Engine) and an Agilent liquid chromatograph - single quadrupole mass spectrometer (LC-MSD). The LC-MSD is currently widely used by a number of investigators for analysis of lipids, steroid hormones, peptides and proteins. These varied needs have created an increasing demand for LC-MS analysis. Therefore, we request the purchase of a LC-TQ MS because of its MS/MS capabilities that will overcome many of the limitations of the current instrument. This MS/MS capability will facilitate the identification of unknown compounds. This function is currently performed by GC-MS, which requires sample derivatization and compatibility with GC conditions. The LC-TQ MS will also facilitate the measurement of analytes with the same m/z since daughter ions of different m/z may be selected for measurement. This will result in increase in confidence in analysis of certain peaks in complex samples. It will also result in shorter run time since the LC-MSD needs a complete resolution of multiple compounds with same m/z. The needs of the 15 principal users of this grant fall into four categories: (1) identification and measurement of eicosanoids, (2) measurement of lipid peroxidation products, isoprostanes and oxylipids, (3) analysis of addictive drugs and their metabolites, and (4) miscellaneous applications. About 75-80% of instrument time will be available to these participants and the remaining time will be available to other investigators at the College.
Funding Period: 2004-04-01 - 2005-03-31
more information: NIH RePORT

Top Publications

  1. ncbi Metabolism of adrenic acid to vasodilatory 1alpha,1beta-dihomo-epoxyeicosatrienoic acids by bovine coronary arteries
    Xiu Yu Yi
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 292:H2265-74. 2007
  2. pmc Diverse roles of 2-arachidonoylglycerol in invasion of prostate carcinoma cells: Location, hydrolysis and 12-lipoxygenase metabolism
    Michael P Endsley
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, USA
    Int J Cancer 121:984-91. 2007
  3. ncbi Adenoviral expression of 15-lipoxygenase-1 in rabbit aortic endothelium: role in arachidonic acid-induced relaxation
    Nitin T Aggarwal
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 292:H1033-41. 2007
  4. ncbi Endothelium-derived steroidogenic factor enhances angiotensin II-stimulated aldosterone release by bovine zona glomerulosa cells
    Craig J Hanke
    Department of Human Biology, University of Wisconsin Green Bay, Wisconsin 54301, USA
    Endocrinology 148:317-23. 2007
  5. pmc Simultaneous analysis of angiotensin peptides by LC-MS and LC-MS/MS: metabolism by bovine adrenal endothelial cells
    Lijie Cui
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Anal Biochem 369:27-33. 2007
  6. ncbi Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta
    Yuttana Chawengsub
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H1348-56. 2008
  7. pmc Angiotensin II relaxations of bovine adrenal cortical arteries: role of angiotensin II metabolites and endothelial nitric oxide
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 52:150-5. 2008

Scientific Experts

  • Kathryn M Gauthier
  • William B Campbell
  • Kasem Nithipatikom
  • Lijie Cui
  • John R Falck
  • Nitin T Aggarwal
  • Yuttana Chawengsub
  • Bruce D Hammock
  • Craig J Hanke
  • Xiu Yu Yi
  • Michael P Endsley
  • Blythe B Holmes
  • Siddam Anjaiah
  • Craig E Wheelock
  • Yafei Xu
  • Helena Viita
  • Nitin Aggarwal
  • Seppo Yla-Herttuala
  • Marilyn A Isbell

Detail Information

Publications7

  1. ncbi Metabolism of adrenic acid to vasodilatory 1alpha,1beta-dihomo-epoxyeicosatrienoic acids by bovine coronary arteries
    Xiu Yu Yi
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 292:H2265-74. 2007
    ..Our results suggest a role of adrenic acid metabolites, specifically, DH-EETs as endothelium-derived hyperpolarizing factors in the coronary circulation...
  2. pmc Diverse roles of 2-arachidonoylglycerol in invasion of prostate carcinoma cells: Location, hydrolysis and 12-lipoxygenase metabolism
    Michael P Endsley
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, USA
    Int J Cancer 121:984-91. 2007
    ..Therefore, inhibitors of 2-AG hydrolysis could potentially serve as therapeutic agents for the treatment of prostate cancer. (c) 2007 Wiley-Liss, Inc...
  3. ncbi Adenoviral expression of 15-lipoxygenase-1 in rabbit aortic endothelium: role in arachidonic acid-induced relaxation
    Nitin T Aggarwal
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 292:H1033-41. 2007
    ..This confirms the role of HEETA and THETA as endothelium-derived relaxing factors...
  4. ncbi Endothelium-derived steroidogenic factor enhances angiotensin II-stimulated aldosterone release by bovine zona glomerulosa cells
    Craig J Hanke
    Department of Human Biology, University of Wisconsin Green Bay, Wisconsin 54301, USA
    Endocrinology 148:317-23. 2007
    ..These studies indicate that EDSF enhances the steroidogenic effect of AII. In the adrenal gland, ECs are in close anatomical relationship with ZG cells and may sensitize ZG cells to the steroidogenic action of AII by releasing EDSF...
  5. pmc Simultaneous analysis of angiotensin peptides by LC-MS and LC-MS/MS: metabolism by bovine adrenal endothelial cells
    Lijie Cui
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Anal Biochem 369:27-33. 2007
    ..The results show that Ang II is metabolized by endothelial cells to Ang 1-7, Ang III, and Ang IV, with Ang 1-7 being the major metabolite...
  6. ncbi Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta
    Yuttana Chawengsub
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H1348-56. 2008
    ..These results indicate that 15-H-11,12-EETA is produced by the aorta, hydrolyzed by sEH to 11,12,15-THETA, and mediates relaxations by membrane hyperpolarization. 15-H-11,12-EETA represents an endothelium-derived hyperpolarizing factor...
  7. pmc Angiotensin II relaxations of bovine adrenal cortical arteries: role of angiotensin II metabolites and endothelial nitric oxide
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 52:150-5. 2008
    ....