Mechanisms of Intercelllar Communication

Summary

Principal Investigator: VYTAUTAS K VERSELIS
Abstract: DESCRIPTION (provided by applicant): This application is for "highly-focused postdoctoral training". The objectives are to: 1) Provide outstanding postdoctoral training in basic research on molecular structure and physiological function of electrical and chemical synapses;2) Capitalize on the synergism and collaboration of participating faculty;3) Relate basic science findings to clinical problems;and 4) Train members of underrepresented minority groups. Strategies to these ends are to: 1) Restrict training faculty to a small, highly-interactive group;2) Recruit and select top quality trainees;3) Actively recruit minority trainees;4) Mandate attendance at departmental seminars, works-in-progress presentations and journal clubs;and 5) Promote collaborative projects with more than one mentor. Training areas will be: i) Connexins: Structure/Function and Electrical Synaptic Transmission. Studies include mechanisms of connexin channel gating and permeability, trafficking, regulation of expression, physiological roles of channels and hemichannels and mechanisms of diseases, //) Molecular/Cellular Physiology and Biophysics of Chemically Mediated Synaptic Transmission. Studies include glutamate and GABA receptor structure-function, pharmacology and trafficking, regulation by kinases, role of receptors in delayed neurodegeneration, inhibitory-excitatory neuron communication and neuronal integration, Hi) Mechanisms of Synaptic Plasticity. Studies include mechanisms of LTP and LTD, role of endogenous cannabinoids and kinases, receptor distribution, cycling and synaptic plasticity at the systems level. Research in the Dominick P. Purpura Department of Neuroscience continues to flourish, with the addition of 6 new faculty members since the last submission. Past and present trainees have been highly productive and show a high level of retention in biomedical research. The College continues to actively support graduate and postgraduate education. Trainees will carry out research under the primary supervision of participating faculty, with an emphasis on collaborative projects that extend the boundaries beyond a single laboratory. Bioethics and scientific conduct are a regular part of training. Trainees are recruited at national scientific meetings, especially the Society for Neuroscience, by announcements in Peterson's Guide and programs published by the Association of Neuroscience Departments/Programs. Additional efforts at minority recruitment involve participation at meetings organized for minority students. Trainees are encouraged to take full advantage of the multiplicity of scientific opportunities in the College as a whole. RELEVANCE (See instructions):
Funding Period: 1998-07-01 - 2014-06-30
more information: NIH RePORT

Top Publications

  1. pmc AMPAR exocytosis through NO modulation of PICK1
    Kenneth G Sossa
    Albert Einstein College of Medicine of Yeshiva University, Dominick P Purpura Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, 1410 Pelham Parkway South, Bronx, NY 10461, USA
    Neuropharmacology 53:92-100. 2007
  2. pmc Contribution of pannexin1 to experimental autoimmune encephalomyelitis
    Sarah E Lutz
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    PLoS ONE 8:e66657. 2013
  3. pmc Pannexin 1 involvement in bladder dysfunction in a multiple sclerosis model
    Hiromitsu Negoro
    Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Sci Rep 3:2152. 2013
  4. pmc The D50N mutation and syndromic deafness: altered Cx26 hemichannel properties caused by effects on the pore and intersubunit interactions
    Helmuth A Sánchez
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 142:3-22. 2013
  5. ncbi Loss of astrocyte connexins 43 and 30 does not significantly alter susceptibility or severity of acute experimental autoimmune encephalomyelitis in mice
    Sarah E Lutz
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA
    J Neuroimmunol 245:8-14. 2012
  6. pmc Targeting pannexin1 improves seizure outcome
    Marcelo F Santiago
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 6:e25178. 2011
  7. pmc mGluR and NMDAR activation internalize distinct populations of AMPARs
    Tanya M Casimiro
    Albert Einstein College of Medicine of Yeshiva University, Dominick P Purpura Department of Neuroscience, Rose Kennedy Center for Mental Retardation, 1410 Pelham Parkway South, Bronx, NY 10461, United States
    Mol Cell Neurosci 48:161-70. 2011
  8. pmc Atypical properties of release and short-term depression at a specialized nicotinic synapse in the Mauthner cell network
    Simon Gelman
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA
    J Exp Biol 214:1560-70. 2011
  9. ncbi Otolith endorgan input to the Mauthner neuron in the goldfish
    Theresa M Szabo
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461, USA
    J Comp Neurol 505:511-25. 2007
  10. pmc Input-specific plasticity at excitatory synapses mediated by endocannabinoids in the dentate gyrus
    Chiayu Q Chiu
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuropharmacology 54:68-78. 2008

Detail Information

Publications11

  1. pmc AMPAR exocytosis through NO modulation of PICK1
    Kenneth G Sossa
    Albert Einstein College of Medicine of Yeshiva University, Dominick P Purpura Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, 1410 Pelham Parkway South, Bronx, NY 10461, USA
    Neuropharmacology 53:92-100. 2007
    ..Our data suggest that NMDAR activation can drive the surface delivery of AMPARs from a pool of intracellular AMPARs retained by PICK1 through the NO-dependent modification of NSF...
  2. pmc Contribution of pannexin1 to experimental autoimmune encephalomyelitis
    Sarah E Lutz
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    PLoS ONE 8:e66657. 2013
    ....
  3. pmc Pannexin 1 involvement in bladder dysfunction in a multiple sclerosis model
    Hiromitsu Negoro
    Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Sci Rep 3:2152. 2013
    ..Mefloquine (Panx1 blocker) reduced these IL-1β responses. We propose that Panx1 signaling provides a positive feedback loop for inflammatory responses involved in bladder dysfunction in MS. ..
  4. pmc The D50N mutation and syndromic deafness: altered Cx26 hemichannel properties caused by effects on the pore and intersubunit interactions
    Helmuth A Sánchez
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 142:3-22. 2013
    ..Collectively, these data provide insight into Cx26 structure-function and the underlying bases for the phenotypes associated with KID syndrome patients carrying the D50N mutation. ..
  5. ncbi Loss of astrocyte connexins 43 and 30 does not significantly alter susceptibility or severity of acute experimental autoimmune encephalomyelitis in mice
    Sarah E Lutz
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA
    J Neuroimmunol 245:8-14. 2012
    ....
  6. pmc Targeting pannexin1 improves seizure outcome
    Marcelo F Santiago
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 6:e25178. 2011
    ..These data thus indicate that the activation of Panx1 in juvenile mouse hippocampi contributes to neuronal hyperactivity in seizures...
  7. pmc mGluR and NMDAR activation internalize distinct populations of AMPARs
    Tanya M Casimiro
    Albert Einstein College of Medicine of Yeshiva University, Dominick P Purpura Department of Neuroscience, Rose Kennedy Center for Mental Retardation, 1410 Pelham Parkway South, Bronx, NY 10461, United States
    Mol Cell Neurosci 48:161-70. 2011
    ....
  8. pmc Atypical properties of release and short-term depression at a specialized nicotinic synapse in the Mauthner cell network
    Simon Gelman
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA
    J Exp Biol 214:1560-70. 2011
    ..We suggest that the second component of depression provides an additional safety factor to prevent repetitive firing of the CRN...
  9. ncbi Otolith endorgan input to the Mauthner neuron in the goldfish
    Theresa M Szabo
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461, USA
    J Comp Neurol 505:511-25. 2007
    ..These results demonstrate that nerves from all three endorgans contact the M-cell, with input-specific segregation of synapses along the M-cell lateral dendrite...
  10. pmc Input-specific plasticity at excitatory synapses mediated by endocannabinoids in the dentate gyrus
    Chiayu Q Chiu
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuropharmacology 54:68-78. 2008
    ..Finally, we find that manipulations known to induce eCB-LTD at other central synapses do not trigger LTD at MCF-DGC synapses...
  11. pmc Connexins modulate autophagosome biogenesis
    Eloy Bejarano
    Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Cell Biol 16:401-14. 2014
    ..Maturation of the Cx-containing pre-autophagosomes into autophagosomes leads to degradation of these endogenous inhibitors, allowing for sustained activation of autophagy. ..