Pacific Southwest RCE for Biodefense &Emerging Infectious Diseases Research

Summary

Principal Investigator: Alan G Barbour
Abstract: Pacific-Southwest Regional Center of Excellence for Biodefense and Emerging Diseases ("the Center") is a research consortium comprising 34 projects clustered within seven programs. The Center also features five differentiated core facilities that serve the entire region. All four states (AZ, CA, HI, and NV) of Region IX are represented among the 27 educational, research, commercial, and governmental institutions of the Center. The overall goal of the Center is outstanding science, achieved with a multidisciplinary and collaborative approach and a portfolio that balances basic and translational research. The Center's major theme is infections of the western United States, the Pacific Rim, and Latin America of relevance for biodefense or as emerging diseases. The seven programs are: (1) dengue, (2) viral zoonoses, including arenaviruses and Nipah virus, (3) botulinum neurotoxin, (4) Category B Burkholderia species, (5) tularemia, (6) coccidioidomycosis, and (7) reservoir-targeted vaccines for prevention of human infections. Applications of the research include improved diagnostics, novel therapeutic agents, and vaccine platforms. Other key functions of the Center are: (a) responding, in cooperation with public health agencies, to infectious diseases emergencies with its collective expertise, resources, and facilities;(b) fostering the careers of talented young investigators and more established investigators newly-entering this research field;and (c) providing a region-wide training facility for investigators and staff to learn safe operations under biocontainment conditions.
Funding Period: 2005-05-20 - 2015-04-30
more information: NIH RePORT

Top Publications

  1. pmc Conserved residues in Lassa fever virus Z protein modulate viral infectivity at the level of the ribonucleoprotein
    Althea A Capul
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Virol 85:3172-8. 2011
  2. pmc Henipavirus receptor usage and tropism
    Olivier Pernet
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1489, USA
    Curr Top Microbiol Immunol 359:59-78. 2012
  3. pmc Genome-wide patterns of intrahuman dengue virus diversity reveal associations with viral phylogenetic clade and interhost diversity
    Poornima Parameswaran
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, USA
    J Virol 86:8546-58. 2012
  4. pmc Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus
    Brook E Heaton
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 287:12098-110. 2012
  5. pmc Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection
    Mariangela Bonizzoni
    Program in Public Health, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 7:e50512. 2012
  6. pmc Contributions of mRNA abundance, ribosome loading, and post- or peri-translational effects to temporal repression of C. elegans heterochronic miRNA targets
    Michael Stadler
    Department of Genetics, Stanford University, Stanford, California 94305 5324, USA
    Genome Res 22:2418-26. 2012
  7. pmc Characterization of ceftazidime resistance mechanisms in clinical isolates of Burkholderia pseudomallei from Australia
    Derek S Sarovich
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
    PLoS ONE 7:e30789. 2012
  8. pmc Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications
    Erin P Price
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
    PLoS ONE 7:e37723. 2012
  9. pmc Resistance to plague among black-tailed prairie dog populations
    Tonie E Rocke
    U S Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711, USA
    Vector Borne Zoonotic Dis 12:111-6. 2012
  10. pmc Structural gene (prME) chimeras of St Louis encephalitis virus and West Nile virus exhibit altered in vitro cytopathic and growth phenotypes
    Payal D Maharaj
    Division of Vector Borne Diseases, Centers for Disease Control and Prevention CDC, Fort Collins, CO 80521, USA
    J Gen Virol 93:39-49. 2012

Detail Information

Publications341 found, 100 shown here

  1. pmc Conserved residues in Lassa fever virus Z protein modulate viral infectivity at the level of the ribonucleoprotein
    Althea A Capul
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    J Virol 85:3172-8. 2011
    ..Our findings provide direct experimental evidence supporting a role for Z in the modulation of the activity of the viral ribonucleoprotein (RNP) complex and its packaging into mature infectious viral particles...
  2. pmc Henipavirus receptor usage and tropism
    Olivier Pernet
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1489, USA
    Curr Top Microbiol Immunol 359:59-78. 2012
    ..and Geisbert et al. in this issue. Here, we will review the biology of the HNV receptors, and how receptor usage relates to HNV cell tropism in vitro and in vivo...
  3. pmc Genome-wide patterns of intrahuman dengue virus diversity reveal associations with viral phylogenetic clade and interhost diversity
    Poornima Parameswaran
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, USA
    J Virol 86:8546-58. 2012
    ....
  4. pmc Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus
    Brook E Heaton
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 287:12098-110. 2012
    ....
  5. pmc Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection
    Mariangela Bonizzoni
    Program in Public Health, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 7:e50512. 2012
    ..These results indicate the necessity of additional studies of mosquito-DENV interactions, specifically those focused on recently-derived mosquito strains with multiple dengue virus serotypes and genotypes...
  6. pmc Contributions of mRNA abundance, ribosome loading, and post- or peri-translational effects to temporal repression of C. elegans heterochronic miRNA targets
    Michael Stadler
    Department of Genetics, Stanford University, Stanford, California 94305 5324, USA
    Genome Res 22:2418-26. 2012
    ....
  7. pmc Characterization of ceftazidime resistance mechanisms in clinical isolates of Burkholderia pseudomallei from Australia
    Derek S Sarovich
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
    PLoS ONE 7:e30789. 2012
    ..pseudomallei and to provide proven and clinically relevant signatures for monitoring the development of antibiotic resistance in this important pathogen...
  8. pmc Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications
    Erin P Price
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
    PLoS ONE 7:e37723. 2012
    ..Using B. pseudomallei as a model, our study provides a framework for comprehensive quantitative validation of molecular assays and provides additional, highly validated B. pseudomallei assays for the scientific research community...
  9. pmc Resistance to plague among black-tailed prairie dog populations
    Tonie E Rocke
    U S Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711, USA
    Vector Borne Zoonotic Dis 12:111-6. 2012
    ..Microsatellite analysis revealed that all three groups were distinct genetically, but further studies are needed to establish a genetic basis for the observed differences in plague resistance...
  10. pmc Structural gene (prME) chimeras of St Louis encephalitis virus and West Nile virus exhibit altered in vitro cytopathic and growth phenotypes
    Payal D Maharaj
    Division of Vector Borne Diseases, Centers for Disease Control and Prevention CDC, Fort Collins, CO 80521, USA
    J Gen Virol 93:39-49. 2012
    ..These data provide genetic insight into divergent enzootic maintenance strategies that could also be useful for the assessment of emergence mechanisms of closely related flaviviruses...
  11. pmc A systems biology starter kit for arenaviruses
    Magali E Droniou-Bonzom
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Viruses 4:3625-46. 2012
    ..In this way, we hope to provide an overview of the interplay between arenaviruses and the host cell, and lay the foundations for complementing current arenavirus research with a systems-level approach...
  12. pmc Francisella tularensis Schu S4 O-antigen and capsule biosynthesis gene mutants induce early cell death in human macrophages
    Stephen R Lindemann
    Department of Microbiology, The University of Iowa, Iowa City, Iowa, USA
    Infect Immun 79:581-94. 2011
    ....
  13. pmc Molecular mechanisms of inflammasome activation during microbial infections
    Petr Broz
    Department of Microbiology and Immunology, Stanford School of Medicine, Stanford University, Stanford, CA 94305, USA
    Immunol Rev 243:174-90. 2011
    ..In addition, we review the role of the inflammasome adapter ASC in caspase-1 autoproteolysis and new insights into the structure of the inflammasome complex...
  14. pmc Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated
    Hua Xin Liao
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    J Exp Med 208:2237-49. 2011
    ..These data suggest that the majority of gp41-binding antibodies produced after acute HIV-1 infection are cross-reactive responses generated by stimulating memory B cells that have previously been activated by non-HIV-1 antigens...
  15. pmc Development of peptide-conjugated morpholino oligomers as pan-arenavirus inhibitors
    Benjamin W Neuman
    University of Reading, Reading RG6 6AJ, United Kingdom
    Antimicrob Agents Chemother 55:4631-8. 2011
    ..These results suggest that arenavirus 5' genomic termini represent promising targets for pan-arenavirus antiviral therapeutic development...
  16. pmc Substitutions in the glycoprotein (GP) of the Candid#1 vaccine strain of Junin virus increase dependence on human transferrin receptor 1 for entry and destabilize the metastable conformation of GP
    Magali E Droniou-Bonzom
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, HMR502, Los Angeles, CA 90033, USA
    J Virol 85:13457-62. 2011
    ..Lastly, we identified an increased dependence of Cd1 GP on human transferrin receptor type 1 (hTfR-1) for entry, which may affect the tropism of the attenuated strain in vivo...
  17. pmc Unusual dengue virus 3 epidemic in Nicaragua, 2009
    Gamaliel Gutierrez
    Sustainable Sciences Institute, Managua, Nicaragua
    PLoS Negl Trop Dis 5:e1394. 2011
    ..This study demonstrates that parameters other than serotype, viral genomic sequence, immune status, and sequence of serotypes can play a role in modulating dengue disease outcome...
  18. pmc Subunit vaccine efficacy against Botulinum neurotoxin subtypes
    James S Henkel
    Medical College of Wisconsin, Microbiology and Molecular Genetics, Milwaukee, WI, USA
    Vaccine 29:7688-95. 2011
    ..These results may provide a reference for the development of pan-BoNT vaccines...
  19. pmc Dermacentor andersoni transmission of Francisella tularensis subsp. novicida reflects bacterial colonization, dissemination, and replication coordinated with tick feeding
    Kathryn E Reif
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 79:4941-6. 2011
    ..The identification of specific genes and, more importantly, conserved pathways that function at the tick-pathogen interface will accelerate the development of new methods to block transmission...
  20. pmc Containing the contagion: treating the virus that inspired the film
    Benhur Lee
    Department of Microbiology, Immunology, and Molecular Genetics and Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095, USA
    Sci Transl Med 3:105fs6. 2011
    ..Postexposure passive immunotherapy protects African green monkeys from lethal challenge with Contagion-related Hendra virus...
  21. pmc Lipid diversity among botulinum neurotoxin-producing clostridia
    Ziqiang Guan
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Microbiology 158:2577-84. 2012
    ..novyi. A strain in group IV had PE, PG and CL, but none of the distinguishing lipids. This work shows that the lipidome of C. botulinum is consistent with its classification by other methods...
  22. pmc Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays
    Yanfeng Zhang
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Anal Biochem 430:185-92. 2012
    ..These simultaneous, rapid, and sensitive assays have the potential to measure botulinum toxins in a high-throughput manner in complex clinical, food, and environmental samples...
  23. pmc Structural insights into RipC, a putative citrate lyase β subunit from a Yersinia pestis virulence operon
    Rodrigo Torres
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:2-7. 2012
    ..Through sequence and structure comparisons with homologous proteins, it is proposed that RipC is a putative CoA- or CoA-derivative binding protein...
  24. pmc N-Glycans on the Nipah virus attachment glycoprotein modulate fusion and viral entry as they protect against antibody neutralization
    Scott B Biering
    Paul G Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA
    J Virol 86:11991-2002. 2012
    ..Altogether, our results suggest that NiV-G N-glycans play a role in shielding virions against antibody neutralization, while modulating cell-cell fusion and viral entry via multiple mechanisms...
  25. pmc The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems
    Kiel Nikolakakis
    Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA
    Mol Microbiol 84:516-29. 2012
    ..pseudomallei strains. These results demonstrate that Burkholderia species contain functional CDI systems, which may confer a competitive advantage to these bacteria...
  26. pmc The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide
    Apichai Tuanyok
    Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA
    PLoS Negl Trop Dis 6:e1453. 2012
    ..We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains...
  27. pmc Regulation of the nucleocytoplasmic trafficking of viral and cellular proteins by ubiquitin and small ubiquitin-related modifiers
    Yao E Wang
    Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA
    Biol Cell 104:121-38. 2012
    ..In this review, we will summarise our current knowledge on the ubiquitin/SUMO-regulated nuclear/subnuclear trafficking of cellular proteins and describe examples of viral exploitation of these pathways...
  28. pmc Francisella infection triggers activation of the AIM2 inflammasome in murine dendritic cells
    Kamila Belhocine
    Department of Microbiology and Immunology, Stanford School of Medicine, Stanford University, Stanford, CA 94305, USA
    Cell Microbiol 14:71-80. 2012
    ..Taken together, our results demonstrate that F. novicida induces inflammasome activation in dendritic cells. The resulting inflammatory cell death may be beneficial to remove the bacterial replicative niche and protect the host...
  29. pmc Purification and characterization of a novel subtype a3 botulinum neurotoxin
    William H Tepp
    Department of Bacteriology, Food Research Institute, University of Wisconsin Madison, Madison, Wisconsin, USA
    Appl Environ Microbiol 78:3108-13. 2012
    ..These results indicate that BoNT/A3 has novel biochemical and pharmacological properties compared to those of other subtype A toxins...
  30. pmc Cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (WEEM)
    Brett A Chromy
    Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
    BMC Microbiol 12:79. 2012
    ..Toward this end, cytokine profiles in human blood were investigated using a human whole blood ex vivo exposure model, called WEEM...
  31. pmc Dominant cross-reactive B cell response during secondary acute dengue virus infection in humans
    Simona Zompi
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA
    PLoS Negl Trop Dis 6:e1568. 2012
    ..In addition, this study enabled the development of increased technical and research capacity of Nicaraguan scientists and the implementation of several new immunological assays in the field...
  32. pmc Comparative transcriptomics of the saprobic and parasitic growth phases in Coccidioides spp
    Emily Whiston
    Department of Plant and Microbial Biology, University of California, Berkeley, California, United States of America
    PLoS ONE 7:e41034. 2012
    ..These observations will impact priorities for future genetics-based studies in Coccidioides and provide context for studies in other fungal pathogens...
  33. pmc Characterization of a model of lethal dengue virus 2 infection in C57BL/6 mice deficient in the alpha/beta interferon receptor
    Susana Orozco
    Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, CA, USA
    J Gen Virol 93:2152-7. 2012
    ....
  34. pmc Experimental infections of the reservoir species Peromyscus leucopus with diverse strains of Borrelia burgdorferi, a Lyme disease agent
    ELISABETH BAUM
    Departments of Microbiology and Molecular Genetics, Medicine, and Ecology and Evolutionary Biology, University of California, Irvine, CA, USA
    MBio 3:e00434-12. 2012
    ..We propose that for B. burgdorferi, there is selection in reservoir species for (i) sequence diversity, as for OspC and VlsE, and (ii) the presence or absence of polymorphisms, as for BBK07 and BBK12...
  35. pmc A naturally occurring mutation within the probe-binding region compromises a molecular-based West Nile virus surveillance assay for mosquito pools (Diptera: Culicidae)
    Aaron C Brault
    Division of Vector Borne Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd, Fort Collins, CO 80521, USA
    J Med Entomol 49:939-41. 2012
    ..The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms...
  36. pmc Distinct human antibody response to the biological warfare agent Burkholderia mallei
    John J Varga
    University of Virginia, Charlottesville, VA, USA
    Virulence 3:510-4. 2012
    ..pseudomallei infections, there were only two highly increased antibodies shared between these two infections. These findings have implications in the diagnosis and treatment of B. mallei and B. pseudomallei infections...
  37. pmc Temporal dynamics of the transcriptional response to dengue virus infection in Nicaraguan children
    Stephen J Popper
    Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America
    PLoS Negl Trop Dis 6:e1966. 2012
    ..Some of these differential features may predict the outcome of DENV infection...
  38. pmc Development of ceftazidime resistance in an acute Burkholderia pseudomallei infection
    Derek S Sarovich
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ, USA
    Infect Drug Resist 5:129-32. 2012
    ..The ability to rapidly identify the emergence of CAZ resistant B. pseudomallei populations in melioidosis patients will allow timely alterations in treatment strategies, thereby improving patient outcomes for this serious disease...
  39. pmc Antigen-specific acquired immunity in human brucellosis: implications for diagnosis, prognosis, and vaccine development
    Anthony P Cannella
    Division of Infectious Diseases, Department of Medicine, University of California San Diego La Jolla, CA, USA
    Front Cell Infect Microbiol 2:1. 2012
    ....
  40. pmc Cysteines in the stalk of the nipah virus G glycoprotein are located in a distinct subdomain critical for fusion activation
    Dianna Maar
    Biotechnology and Bioengineering Department, Sandia National Laboratories, Livermore, California, USA
    J Virol 86:6632-42. 2012
    ..Our data propose that the cysteine cluster in the NiV-G stalk functions to maintain oligomeric stability but is more importantly involved in stabilizing a unique microdomain critical for triggering fusion...
  41. pmc Development of a high-throughput microsphere-based molecular assay to identify 15 common bloodmeal hosts of Culex mosquitoes
    T C Thiemann
    Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, One Shields Ave, Davis, CA 95616, USA
    Mol Ecol Resour 12:238-46. 2012
    ..bloodmeals to extend our understanding of WNV transmission patterns...
  42. pmc Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 49:345-53. 2011
    ....
  43. pmc Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1
    Christina L Pier
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    FEBS Lett 585:199-206. 2011
    ..These results have important implications for the development of new BoNT based therapeutics and BoNT countermeasures...
  44. ncbi Ultrasensitive detection of botulinum neurotoxins and anthrax lethal factor in biological samples by ALISSA
    Karine Bagramyan
    Department of Immunology, The Beckman Research Institute of the City of Hope, Duarte, CA, USA
    Methods Mol Biol 739:23-36. 2011
    ..A detailed protocol to conduct ALISSA for the detection and quantification of BoNT serotypes A and E and anthrax lethal factor is described...
  45. pmc Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport
    Giulia Pasqual
    Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland
    PLoS Pathog 7:e1002232. 2011
    ..Our data further suggest that the virus-receptor complexes undergo sorting into ILV of the MVB mediated by the ESCRT, possibly using a pathway that may be linked to the cellular trafficking and degradation of the cellular receptor...
  46. pmc Toxin on a stick: modular CDI toxin delivery systems play roles in bacterial competition
    Stephanie K Aoki
    Molecular Cellular and Developmental Biology, University of California, Santa Barbara, CA, USA
    Virulence 2:356-9. 2011
    ..A detailed mechanistic understanding of CDI could facilitate development of probiotics and antimicrobials that target specific pathogens...
  47. pmc Photothermal nanoblade for large cargo delivery into mammalian cells
    Ting Hsiang Wu
    Department of Electrical Engineering, University of California, Los Angeles UCLA, Los Angeles, California 90095, USA
    Anal Chem 83:1321-7. 2011
    ..Overall, the photothermal nanoblade is a new approach for delivering difficult cargo into mammalian cells...
  48. pmc Shared ancestry between a newfound mole-borne hantavirus and hantaviruses harbored by cricetid rodents
    Hae Ji Kang
    Department of Pediatrics and Tropical Medicine, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Virol 85:7496-503. 2011
    ..Our findings warrant more-detailed investigations on the dynamics of spillover and cross-species transmission of present-day hantaviruses within communities of rodents and moles...
  49. pmc Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection
    Maya F Kotturi
    Pfizer Inc, Vaccine Research West, 10777 Science Center Drive, San Diego, CA 92121, USA
    J Virol 85:11770-80. 2011
    ..These data demonstrate for the first time LCMV-specific CD8(+) and CD4(+) T cells in infected humans and begin to provide new insights into memory T cell responses following an acute virus infection...
  50. pmc The public health impact of coccidioidomycosis in Arizona and California
    Richard F Hector
    Global Health Sciences, University of California, San Francisco, 1200 Beale St, 1200, San Francisco, CA 94105, USA
    Int J Environ Res Public Health 8:1150-73. 2011
    ..Similarly, the inability of currently available therapeutics to reduce the duration and morbidity of this disease underscores the need for improved therapeutics and a preventive vaccine...
  51. pmc Independent subtilases expansions in fungi associated with animals
    Anna Muszewska
    Institute of Biochemistry and Biophysics, Warsaw, Poland
    Mol Biol Evol 28:3395-404. 2011
    ..Instead, we hypothesize that the number of fungal serine proteases in a species is related to the use of the animal as a food source, whether it is dead or alive...
  52. pmc Modes of paramyxovirus fusion: a Henipavirus perspective
    Benhur Lee
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, USA
    Trends Microbiol 19:389-99. 2011
    ....
  53. pmc Structural characterization of three novel hydroxamate-based zinc chelating inhibitors of the Clostridium botulinum serotype A neurotoxin light chain metalloprotease reveals a compact binding site resulting from 60/70 loop flexibility
    Aaron A Thompson
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, United States
    Biochemistry 50:4019-28. 2011
    ..The 60/70 loop at the opening of the active site pocket undergoes the largest conformational change, presumably through an induced-fit mechanism, resulting in the most compact catalytic pocket observed in all known BoNT/A LC structures...
  54. pmc Tracking protein aggregate interactions
    Christina J Sigurdson
    Department of Pathology, University of California San Diego, La Jolla, CA, USA red b sheets aligne
    Prion 5:52-5. 2011
    ..Here we focus on the interactions reported to occur when two pre-existing amyloids or two distinct prion strains occur together in the central nervous system...
  55. pmc A naturally derived outer-membrane vesicle vaccine protects against lethal pulmonary Burkholderia pseudomallei infection
    Wildaliz Nieves
    Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA
    Vaccine 29:8381-9. 2011
    ..pseudomallei that can be exploited for other intracellular respiratory pathogens, including other Burkholderia and bacteria capable of establishing persistent infection...
  56. pmc Knockout and pullout recombineering for naturally transformable Burkholderia thailandensis and Burkholderia pseudomallei
    Yun Kang
    Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii, USA
    Nat Protoc 6:1085-104. 2011
    ....
  57. pmc Phylogeography and molecular epidemiology of Yersinia pestis in Madagascar
    Amy J Vogler
    Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, USA
    PLoS Negl Trop Dis 5:e1319. 2011
    ..pestis in Madagascar...
  58. pmc Identification of functional toxin/immunity genes linked to contact-dependent growth inhibition (CDI) and rearrangement hotspot (Rhs) systems
    Stephen J Poole
    Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, USA
    PLoS Genet 7:e1002217. 2011
    ..Together, these results suggest that Rhs plays a role in intercellular competition and that orphan gene modules expand the diversity of toxic activities deployed by both CDI and Rhs systems...
  59. pmc Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors
    Andrew P Liguori
    Department of Biological Sciences, Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
    PLoS ONE 6:e29323. 2011
    ..mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species...
  60. pmc RNA-seq analyses of blood-induced changes in gene expression in the mosquito vector species, Aedes aegypti
    Mariangela Bonizzoni
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA
    BMC Genomics 12:82. 2011
    ..The mosquito Aedes aegypti (Diptera, Culicidae), a vector of Dengue viruses, Yellow Fever Virus (YFV) and Chikungunya virus (CV), is the subject of this study to look at genome-wide changes in gene expression following a blood meal...
  61. pmc Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas
    Aaron C Brault
    Division of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA
    Am J Trop Med Hyg 85:758-67. 2011
    ..The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed...
  62. pmc North American West Nile virus genotype isolates demonstrate differential replicative capacities in response to temperature
    Christy C Andrade
    Center for Vector Borne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA
    J Gen Virol 92:2523-33. 2011
    ..These data indicate that the NS1 and NS4A mutations identified in some WN02 isolates could reduce thermal stability and impede replication of virus at temperatures observed in febrile avian hosts...
  63. pmc Identification of circulating bacterial antigens by in vivo microbial antigen discovery
    Dana E Nuti
    Department of Microbiology and Immunology, University of Nevada School of Medicine, Reno, Nevada, USA
    MBio 2:. 2011
    ..Termed InMAD (in vivo microbial antigen discovery), the strategy has the potential for application to a broad spectrum of microbial pathogens...
  64. pmc Experimental annotation of the human pathogen Histoplasma capsulatum transcribed regions using high-resolution tiling arrays
    Mark Voorhies
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143, USA
    BMC Microbiol 11:216. 2011
    ..A functional interrogation of the Histoplasma genome provides critical support for continued investigation into the biology and pathogenesis of H. capsulatum and related fungi...
  65. pmc Biochemical, structural and molecular dynamics analyses of the potential virulence factor RipA from Yersinia pestis
    Rodrigo Torres
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 6:e25084. 2011
    ..Thus, the results of this molecular study of Y. pestis RipA provide a structural platform for rational inhibitor design, which may lead to a greater understanding of the role of RipA in this unique virulence pathway...
  66. pmc Hendra and nipah infection: pathology, models and potential therapies
    Frederic Vigant
    Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA, USA
    Infect Disord Drug Targets 11:315-36. 2011
    ..We will focus on small molecules or biologics, rather than vaccine strategies, that have been developed as anti-henipaviral therapeutics...
  67. pmc Elevated AIM2-mediated pyroptosis triggered by hypercytotoxic Francisella mutant strains is attributed to increased intracellular bacteriolysis
    Kaitian Peng
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    Cell Microbiol 13:1586-600. 2011
    ....
  68. pmc Purification, modeling, and analysis of botulinum neurotoxin subtype A5 (BoNT/A5) from Clostridium botulinum strain A661222
    Mark J Jacobson
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Appl Environ Microbiol 77:4217-22. 2011
    ..This study reports the first purification of BoNTA5 and describes distinct differences in properties between BoNT/A5 and BoNT/A1...
  69. ncbi A convenient homogeneous enzyme immunoassay for estradiol detection
    May L Chiu
    Chemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA 90095 1592, USA
    Biotechnol Appl Biochem 58:75-82. 2011
    ..7 µM estradiol. A detection limit of 11.5 nM in estradiol-spiked human serum samples suggests the feasibility of applying this assay to monitor estradiol levels for the prediction and prevention of ovarian hyperstimulation syndrome...
  70. pmc Identification of CD4+ T cell epitopes in C. burnetii antigens targeted by antibody responses
    Chen Chen
    Department of Microbial and Molecular Pathogenesis, Texas A and M Health Science Center, College Station, Texas, United States of America
    PLoS ONE 6:e17712. 2011
    ..This suggests that a complete map of CD4(+) response targets in PI-WCV vaccinated mice will likely include antigens against which no antibody responses are made...
  71. pmc Triggering of the newcastle disease virus fusion protein by a chimeric attachment protein that binds to Nipah virus receptors
    Anne M Mirza
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 286:17851-60. 2011
    ....
  72. pmc Comparison of oral toxicological properties of botulinum neurotoxin serotypes A and B
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, U S Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA
    Toxicon 58:62-7. 2011
    ..Dissecting the complexities of the different BoNT serotypes and their roles in foodborne botulism will lead to a better understanding of toxin biology and aid future food risk assessments...
  73. pmc Evaluation of the traditional and revised WHO classifications of Dengue disease severity
    Federico Narvaez
    Infectious Diseases Unit, Hospital Infantil Manuel de Jesus Rivera, Managua, Nicaragua
    PLoS Negl Trop Dis 5:e1397. 2011
    ....
  74. pmc STAT2 mediates innate immunity to Dengue virus in the absence of STAT1 via the type I interferon receptor
    Stuart T Perry
    Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, California, USA
    PLoS Pathog 7:e1001297. 2011
    ..Collectively, these results help elucidate the nature of the poorly understood STAT1-independent host defense mechanism against viruses by identifying a functional type I IFN/STAT2 signaling pathway following DENV infection in vivo...
  75. ncbi An influenza hemagglutinin A peptide assay based on the enzyme-multiplied immunoassay technique
    May L Chiu
    Chemical and Biomolecular Engineering Department, University of California Los Angeles, CA 90095, USA
    J Immunoassay Immunochem 32:1-17. 2011
    ..Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection...
  76. pmc Spongiform encephalopathy in transgenic mice expressing a point mutation in the β2-α2 loop of the prion protein
    Christina J Sigurdson
    Department of Pathology, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 31:13840-7. 2011
    ..These results add to the evidence that the β2-α2 loop has an important role in intermolecular interactions, including that it may be a key determinant of prion protein aggregation...
  77. pmc Emerging paramyxoviruses: molecular mechanisms and antiviral strategies
    Hector C Aguilar
    Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, CA 90095, USA
    Expert Rev Mol Med 13:e6. 2011
    ..This review focuses on the molecular mechanisms discovered and the antiviral strategies pursued in recent years for emerging paramyxoviruses, with particular emphasis on viral entry and exit mechanisms...
  78. pmc Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans
    Li Liang
    Department of Medicine, Division of Infectious Diseases, University of California, Irvine, California 92697, United States
    J Proteome Res 10:4813-24. 2011
    ..This systems biology approach provides an empirical basis for understanding the breadth and specificity of the immune response to B. melitensis and a new framework for comparing the humoral responses against other microorganisms...
  79. pmc The Burkholderia pseudomallei Δasd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice
    Michael H Norris
    Department of Microbiology, University of Hawaii at Manoa, Manoa, HI, 96822, USA
    Infect Immun 79:4010-8. 2011
    ..Thus, the B. pseudomallei Δasd mutant may be a promising live attenuated vaccine strain and a biosafe strain for consideration of exclusion from the select agent list...
  80. pmc Spatially disaggregated disease transmission risk: land cover, land use and risk of dengue transmission on the island of Oahu
    Sophie O Vanwambeke
    Georges Lemaître Centre for Earth and Climate Research, Earth and Life Institute, Universite Catholique de Louvain, Louvain la Neuve, Belgium
    Trop Med Int Health 16:174-85. 2011
    ..The resulting high-resolution maps indicate a high spatial variability in vector-to-host ratio suggesting that risk of exposure is spatially heterogeneous and varies according to land cover and land use...
  81. pmc Proteomics analyses of the opportunistic pathogen Burkholderia vietnamiensis using protein fractionations and mass spectrometry
    Samanthi Wickramasekara
    Department of Chemistry and Biochemistry, University of Arizona, 1306 E University Bolevard, Tucson, AZ 85721, USA
    J Biomed Biotechnol 2011:701928. 2011
    ..Several virulence factors were detected including flagellin, porin, peroxiredoxin and zinc proteases...
  82. pmc Glycosylation modulates arenavirus glycoprotein expression and function
    Cyrille J Bonhomme
    University of California, Irvine, Department of Molecular Biology and Biochemistry and Division of Infectious Disease, 3205 McGaugh Hall, Irvine, CA 92697 3900, USA
    Virology 409:223-33. 2011
    ..We also confirmed the role of the N-linked glycan at position 173 in the masking of the neutralizing epitope GP-1D. Taken together, our results indicated a strong relationship between fusion and infectivity...
  83. ncbi Population differences in host immune factors may influence survival of Gunnison's prairie dogs (Cynomys gunnisoni) during plague outbreaks
    Joseph D Busch
    Center for Microbial Genetics and Genomics, Northern Arizona University, PO Box 4073, Flagstaff, Arizona 86011, USA
    J Wildl Dis 47:968-73. 2011
    ..These results suggest that AV and SE animals might differ in their ability to mount an immune response...
  84. pmc Transcript amplification from single bacterium for transcriptome analysis
    Yun Kang
    Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA
    Genome Res 21:925-35. 2011
    ..thailandensis sequences with no contamination. These data indicate that RNA-seq of TTA from a single cell is possible using this novel method...
  85. pmc Protection by immunoglobulin dual-affinity retargeting antibodies against dengue virus
    James D Brien
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    J Virol 87:7747-53. 2013
    ..In this study, we demonstrate the binding, neutralizing activity, and therapeutic capacity of a novel bispecific dual-affinity retargeting molecule (DART) that limits infection of all four serotypes of dengue virus...
  86. pmc Elevated carbon monoxide in the exhaled breath of mice during a systemic bacterial infection
    Alan G Barbour
    Departments of Medicine and Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, USA
    PLoS ONE 8:e69802. 2013
    ....
  87. pmc miRNA genes of an invasive vector mosquito, Aedes albopictus
    Jinbao Gu
    Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Department of Pathogen Biology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong, P R China
    PLoS ONE 8:e67638. 2013
    ..The distribution of lineage-specific miRNAs is consistent with mosquito phylogeny and the presence of a number of Aedes-specific miRNAs likely reflects the divergence between the Aedes and Culex genera. ..
  88. pmc A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen
    Sinem Beyhan
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, United States of America
    PLoS Biol 11:e1001614. 2013
    ..Taken together, our results reveal a positive feedback circuit that directs a broad transcriptional switch between environmental and pathogenic states in response to temperature. ..
  89. pmc Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity
    Kwangkook Lee
    Department of Physiology and Biophysics, University of California, Irvine, California, United States of America
    PLoS Pathog 9:e1003690. 2013
    ..We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry. ..
  90. pmc PPARγ-mediated increase in glucose availability sustains chronic Brucella abortus infection in alternatively activated macrophages
    Mariana N Xavier
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA 95616, USA
    Cell Host Microbe 14:159-70. 2013
    ..Thus, a shift in intracellular nutrient availability induced by PPARγ promotes chronic persistence of B. abortus within AAMs, and targeting this pathway may aid in eradicating chronic infection. ..
  91. pmc The intracellular cargo receptor ERGIC-53 is required for the production of infectious arenavirus, coronavirus, and filovirus particles
    Joseph P Klaus
    Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA Cellular, Molecular and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405, USA
    Cell Host Microbe 14:522-34. 2013
    ..Thus, we have identified a class of pathogen-derived ERGIC-53 ligands, a lectin-independent basis for their association with ERGIC-53, and a role for ERGIC-53 in the propagation of several highly pathogenic RNA virus families...
  92. pmc Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc
    Mary N Burtnick
    Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama, United States of America
    PLoS ONE 8:e76767. 2013
    ..pseudomallei, but not for B. thailandensis. Collectively, our findings indicate that in addition to being positively regulated by VirAG, B. mallei and B. pseudomallei T6SS-1 gene expression is negatively regulated by iron and zinc. ..
  93. pmc Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron
    Elisa Deriu
    Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697 4025, USA
    Cell Host Microbe 14:26-37. 2013
    ..coli Nissle in lipocalin 2-deficient mice. Thus, iron availability impacts S. Typhimurium growth, and E. coli Nissle reduces S. Typhimurium intestinal colonization by competing for this limiting nutrient. ..
  94. pmc Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California 94710, United States
    J Agric Food Chem 61:755-60. 2013
    ..Both the ELISA and the ECL immunoassay platforms are fast, simple alternatives for use in the routine detection of BoNTs in food and animal sera...
  95. pmc Burkholderia BcpA mediates biofilm formation independently of interbacterial contact-dependent growth inhibition
    Erin C Garcia
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, 27599, USA
    Mol Microbiol 89:1213-25. 2013
    ..We propose, therefore, that bacteria may use CDI proteins in cooperative behaviours, like building biofilm communities, and in competitive behaviours that prevent non-self bacteria from entering the community...
  96. pmc Genetic analysis of invasive Aedes albopictus populations in Los Angeles County, California and its potential public health impact
    Daibin Zhong
    Program in Public Health, College of Health Sciences, University of California Irvine, Irvine, California, USA
    PLoS ONE 8:e68586. 2013
    ..There remains an imperative need for improved surveillance and control strategies for this species. ..
  97. pmc Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples
    Karine Bagramyan
    Beckman Research Institute of City of Hope, Department of Immunology, Duarte, CA 91010, USA
    Anal Chem 85:5569-76. 2013
    ..Redistribution and elevation rates for active toxin were measured and are comparable to those reported for inactive toxin...
  98. pmc Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A)
    Birgitte P S Jacky
    Allergan, Department of Biological Sciences, Irvine, California, United States of America
    PLoS Pathog 9:e1003369. 2013
    ..These findings show that FGFR3 plays a pivotal role in the specific uptake of BoNT/A across the cell membrane being part of a larger receptor complex involving ganglioside- and protein-protein interactions...
  99. pmc Functional characterization of Burkholderia pseudomallei trimeric autotransporters
    Cristine G Campos
    Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    Infect Immun 81:2788-99. 2013
    ..At 48 h postinoculation, only one strain, Bp340::pDbpaC, demonstrated a defect in dissemination and/or survival in the liver, indicating that BpaC is required for wild-type virulence in this model. ..
  100. pmc Development of an internally controlled real-time reverse transcriptase PCR assay for pan-dengue virus detection and comparison of four molecular dengue virus detection assays
    Jesse J Waggoner
    Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA
    J Clin Microbiol 51:2172-81. 2013
    ..The detection of DENV RNA late in the course of clinical illness should serve to lengthen the period during which a confirmed molecular diagnosis of DENV infection can be provided...

Research Grants31

  1. Pacific NorthWest Regional Center of Excellence (PNWRCE)
    Jay A Nelson; Fiscal Year: 2013
    ..pseudomallei host pathogen response during both the septicemic as well as the intracellular phases of the disease. ..
  2. Southeast Regional Centers of Excellence for Biodefense &Emerging Infectious Di
    Philip Frederick Sparling; Fiscal Year: 2013
    ..SERCEB brings new investigators to the biodefense effort through a combination of educational programs, support of innovative new projects, and the synergistic interactions among its world-class investigators. ..
  3. New England Regional Center of Excellence in Biodefense and Emerging Infectious D
    Dennis L Kasper; Fiscal Year: 2013
    ..NERCE will also continue its Developmental Projects program and Career Development in Biodefense program in an effort to initiate new research efforts and to attract new investigators to this field. ..
  4. Northeast Biodefense Center
    W Ian Lipkin; Fiscal Year: 2013
    ..As a Center based in a School of Public Health and a State Department of Health, the NBC has a firm commitment to and practical understanding of Emergency Preparedness. ..
  5. Molecular Analyses and Interventions for Biodefense and Emerging Pathogens
    Olaf Schneewind; Fiscal Year: 2013
    ..Research and training at the GLRCE is governed by a mechanism involving ongoing review of scientific excellence and translational goals, inter-institutional advisory boards and external scientific advisory bodies. ..