Bacillus thermoproteolyticus

Summary

Alias: 'Bacillus thermoproteolyticus'

Top Publications

  1. pmc Vibrio cholerae hemagglutinin(HA)/protease: An extracellular metalloprotease with multiple pathogenic activities
    Jorge A Benitez
    Morehouse School of Medicine Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr, SW Atlanta, GA, 30310, USA Electronic address
    Toxicon 115:55-62. 2016
  2. doi Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation
    Mami Dendo
    1Division of Advanced Surgical Science and Technology, Tohoku University School of Medicine, Sendai, Japan 2New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan 3Graduate school of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan 4Graduate school of Biomedical Engineering, Tohoku University, Sendai, Japan 5Department of Integrative Genomics, Tohoku Medical Megabank Organization ToMMO, Tohoku University School of Medicine, Sendai, Japan 6Department of Biochemistry, Center for Regulatory Epigenome and Diseases, Tohoku University Graduated School of Medicine, Sendai, Japan
    Transplantation 99:1349-55. 2015
  3. doi Enzymatically triggered peptide hydrogels for 3D cell encapsulation and culture
    Laura Szkolar
    School of Materials, The University of Manchester, Manchester, M13 9PL, UK Manchester Institute of Biotechnology, The University of Manchester, Manchester, M13 9PL, UK
    J Pept Sci 20:578-84. 2014
  4. pmc Functional analysis of the Burkholderia cenocepacia ZmpA metalloprotease
    C Kooi
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 187:4421-9. 2005
  5. ncbi The protein-protein interactions between SMPI and thermolysin studied by molecular dynamics and MM/PBSA calculations
    Olayiwola A Adekoya
    Department of Pharmacology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N 9037 Tromsø, Norway
    J Biomol Struct Dyn 22:521-31. 2005
  6. ncbi Gene cloning and characterization of a Bacillus vietnamensis metalloprotease
    Mihwan Kim
    Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Biosci Biotechnol Biochem 68:1533-40. 2004
  7. doi Effect of enzyme concentration of the morphology and properties of enzymatically triggered peptide hydrogels
    Jean Baptiste Guilbaud
    School of Materials, University of Manchester, Manchester, M13 9PL, United Kingdom
    Biomacromolecules 14:1403-11. 2013
  8. ncbi Purification and characterization of a thermolysin like protease from Thermoactinomyces thalpophilus MCMB-380
    Devipriya R Majumder
    Microbial Sciences Division, MACS Agharkar Research Institute, G G Agarkar Road, Pune 411004, India
    Protein Pept Lett 20:918-25. 2013
  9. pmc A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products
    A N Balamurugan
    Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 93:693-702. 2012
  10. ncbi The roles of the prosequence of thermolysin in enzyme inhibition and folding in vitro
    M J O'Donohue
    Departement de Pharmacochimie Moleculaire et Structurale, U266 INSERM, Paris, France
    J Biol Chem 271:26477-81. 1996

Patents

  1. Protease glue from Bacillus thermoproteolyticus rokko, procedure for obtaining it and its use

Scientific Experts

  • K Inouye
  • JORGE ANTONIO BENITEZ
  • Tadashi Hatanaka
  • Jean François Gaucher
  • Keiichi Fukuyama
  • Jean Baptiste Guilbaud
  • Mami Dendo
  • Laura Szkolar
  • Alberto Saiani
  • Aline F Miller
  • Devipriya R Majumder
  • A N Balamurugan
  • Hiroaki Hasegawa
  • Mahmud Tareq Hassan Khan
  • Minghui Dong
  • Ingebrigt Sylte
  • Kiyoshi Yasukawa
  • C Kooi
  • Olayiwola A Adekoya
  • Tadayoshi Shirakawa
  • Mihwan Kim
  • Akiko Inagaki
  • Noriaki Ohuchi
  • Youhei Yamagata
  • Hiroshi Maeda
  • Takehiro Imura
  • Kimiko Watanabe
  • Yasutake Katoh
  • Keisei Fujimori
  • Susumu Satomi
  • Kazutaka Murayama
  • Yasuhiro Igarashi
  • Masayuki Ebina
  • Masafumi Goto
  • Kazuhiko Igarashi
  • Julie E Gough
  • Cyrille Rochas
  • Pradnya P Kanekar
  • Sushama M Gaikwad
  • Klearchos K Papas
  • Bernhard J Hering
  • Sajjad M Soltani
  • James Harmon
  • Melena D Bellin
  • Joshua J Wilhelm
  • Mukesh Tiwari
  • Gopalakrishnan Loganathan
  • David M Radosevich
  • Takeshi Yuasa
  • Robert McCarthy
  • Takayuki Anazawa
  • David E R Sutherland
  • Dima N Gharaibeh
  • CLAUDIA C HASE
  • Erin J Lind
  • Haiyan Liu
  • Masayuki Kusano
  • A Banbula
  • Nils Peder Willassen
  • Yasuhiro Takahashi
  • Kei Wada
  • C R Corbett
  • Daijiro Ohmori
  • Toshifumi Takao
  • P A Sokol
  • Junko Hirota
  • Yoshitaka Nishiyama
  • Kiyoshi Mura
  • Soichi Arai
  • Chiyoko Tokue
  • M J O'Donohue
  • W Bode
  • J Travis
  • J Potempa
  • R Huber
  • K Mann
  • C Fernandez-Catalan
  • F Medrano
  • A Beaumont
  • D R Holland
  • R Langen
  • D E Tronrud
  • D B McKay
  • S R Kyöstiö
  • R A Bever
  • P J Stephens
  • G M Jensen
  • H W Pley
  • B W Matthews
  • A Warshel

Detail Information

Publications27

  1. pmc Vibrio cholerae hemagglutinin(HA)/protease: An extracellular metalloprotease with multiple pathogenic activities
    Jorge A Benitez
    Morehouse School of Medicine Department of Microbiology, Biochemistry and Immunology, 720 Westview Dr, SW Atlanta, GA, 30310, USA Electronic address
    Toxicon 115:55-62. 2016
    ..and displays significant amino acid sequence homology to Pseudomonas aeruginosa elastase (LasB) and Bacillus thermoproteolyticus thermolysin...
  2. doi Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation
    Mami Dendo
    1Division of Advanced Surgical Science and Technology, Tohoku University School of Medicine, Sendai, Japan 2New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan 3Graduate school of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan 4Graduate school of Biomedical Engineering, Tohoku University, Sendai, Japan 5Department of Integrative Genomics, Tohoku Medical Megabank Organization ToMMO, Tohoku University School of Medicine, Sendai, Japan 6Department of Biochemistry, Center for Regulatory Epigenome and Diseases, Tohoku University Graduated School of Medicine, Sendai, Japan
    Transplantation 99:1349-55. 2015
    ..Thermolysin (TL) from Bacillus thermoproteolyticus is the gold standard neutral protease...
  3. doi Enzymatically triggered peptide hydrogels for 3D cell encapsulation and culture
    Laura Szkolar
    School of Materials, The University of Manchester, Manchester, M13 9PL, UK Manchester Institute of Biotechnology, The University of Manchester, Manchester, M13 9PL, UK
    J Pept Sci 20:578-84. 2014
    ..of FEFK (F, phenylalanine; E, glutamic acid; K, lysine) using thermolysin, a protease enzyme from Bacillus Thermoproteolyticus Rokko, we have investigated the possibility of using this gelation triggering mechanism to encapsulate ..
  4. pmc Functional analysis of the Burkholderia cenocepacia ZmpA metalloprotease
    C Kooi
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1
    J Bacteriol 187:4421-9. 2005
    ..as a preproenzyme typical of thermolysin-like proteases such as Pseudomonas aeruginosa LasB and Bacillus thermoproteolyticus thermolysin...
  5. ncbi The protein-protein interactions between SMPI and thermolysin studied by molecular dynamics and MM/PBSA calculations
    Olayiwola A Adekoya
    Department of Pharmacology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N 9037 Tromsø, Norway
    J Biomol Struct Dyn 22:521-31. 2005
    Thermolysin is a zinc-metalloendopeptidase secreted by the gram-positive thermophilic bacterium Bacillus thermoproteolyticus. Thermolysin belongs to the gluzinicin family of enzymes, which is selectively inhibited by Steptomyces ..
  6. ncbi Gene cloning and characterization of a Bacillus vietnamensis metalloprotease
    Mihwan Kim
    Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Biosci Biotechnol Biochem 68:1533-40. 2004
    ..It shares 57% amino acid identity with thermolysin originating from Bacillus thermoproteolyticus. The three-dimensional structure of BVMP was deduced by computer-aided modeling with the use of the ..
  7. doi Effect of enzyme concentration of the morphology and properties of enzymatically triggered peptide hydrogels
    Jean Baptiste Guilbaud
    School of Materials, University of Manchester, Manchester, M13 9PL, United Kingdom
    Biomacromolecules 14:1403-11. 2013
    We have recently shown that thermolysine, a protease enzyme obtained from Bacillus thermoproteolyticus rokko , can be used to trigger the gelation of FEFK (F, phenylalanine; E, glutamic acid; K, lysine) tetrapeptides through reverse ..
  8. ncbi Purification and characterization of a thermolysin like protease from Thermoactinomyces thalpophilus MCMB-380
    Devipriya R Majumder
    Microbial Sciences Division, MACS Agharkar Research Institute, G G Agarkar Road, Pune 411004, India
    Protein Pept Lett 20:918-25. 2013
    ..The one hour time-frame is significantly faster than that of the enzyme thermolysin from Bacillus thermoproteolyticus. Moreover the TLP was stable at 80°C for one hour which makes it industrially robust...
  9. pmc A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products
    A N Balamurugan
    Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 93:693-702. 2012
    ..The ECs consisted of purified, intact or truncated class 1 (C1) and class 2 (C2) collagenases from Clostridium histolyticum (Ch), and neutral protease (NP) from Bacillus thermoproteolyticus rokko (thermolysin) or Ch (ChNP).
  10. ncbi The roles of the prosequence of thermolysin in enzyme inhibition and folding in vitro
    M J O'Donohue
    Departement de Pharmacochimie Moleculaire et Structurale, U266 INSERM, Paris, France
    J Biol Chem 271:26477-81. 1996
    The zinc endopeptidase thermolysin (EC 3.4.24.27), an extracellular enzyme from Bacillus thermoproteolyticus, is synthesized as a preproprotein, with the prosequence (204 residues) being two-thirds the size of the mature enzyme (316 ..
  11. ncbi A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli
    Kiyoshi Yasukawa
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Protein Eng Des Sel 20:375-83. 2007
    Thermolysin, a representative zinc metalloproteinase from Bacillus thermoproteolyticus, is synthesized as inactive pre-proenzyme and receives autocatalytic cleavage of the peptide bond linking the pro- and mature sequences...
  12. ncbi Protein control of iron-sulfur cluster redox potentials
    R Langen
    Department of Chemistry, University of Southern California, Los Angeles 90089 0482
    J Biol Chem 267:25625-7. 1992
    ..characterized proteins: Azotobacter vinelandii ferredoxin I, Peptococcus aerogenes ferredoxin, Bacillus thermoproteolyticus ferredoxin, and Chromatium vinosum high potential iron protein (HiPIP)...
  13. pmc Erwinia carotovora subsp. carotovora extracellular protease: characterization and nucleotide sequence of the gene
    S R Kyöstiö
    Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg 24061
    J Bacteriol 173:6537-46. 1991
    ..The deduced amino acid sequence of ORF1 showed significant sequence identity to metalloproteases from Bacillus thermoproteolyticus (thermolysin), B...
  14. ncbi Structure of [4Fe-4S] ferredoxin from Bacillus thermoproteolyticus refined at 2.3 A resolution. Structural comparisons of bacterial ferredoxins
    K Fukuyama
    Department of Biology, Faculty of Science, Osaka University, Japan
    J Mol Biol 210:383-98. 1989
    The structure of a low-potential ferredoxin isolated from Bacillus thermoproteolyticus has been refined by a restrained least-squares method. The final crystallographic R factor is 0...
  15. ncbi Determinants for psychrophilic and thermophilic features of metallopeptidases of the M4 family
    Mahmud Tareq Hassan Khan
    Department of Pharmacology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Tromsø, Norway
    In Silico Biol 9:105-24. 2009
    ..possible determinants of psychrophilic and thermophilic features, by comparing with thermolysin from Bacillus thermoproteolyticus, the prototype enzyme of the family...
  16. doi Virulence of metalloproteases produced by Vibrio species on Pacific oyster Crassostrea gigas larvae
    Hiroaki Hasegawa
    Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon 97331, USA
    Dis Aquat Organ 85:123-31. 2009
    ..Purified V. tubiashii protease A (VtpA), but not the prototype metalloprotease, thermolysin from Bacillus thermoproteolyticus, was highly toxic to the larvae...
  17. doi Origins of the different metal preferences of Escherichia coli peptide deformylase and Bacillus thermoproteolyticus thermolysin: a comparative quantum mechanical/molecular mechanical study
    Minghui Dong
    Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
    J Phys Chem B 112:10280-90. 2008
    The Escherichia coli peptide deformylase (PDF) and Bacillus thermoproteolyticus thermolysin (TLN) are two representative metal-requiring peptidases having remarkably similar active centers but distinctively different metal preferences...
  18. ncbi Engineering, expression, purification, and production of recombinant thermolysin
    Kuniyo Inouye
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biotechnol Annu Rev 13:43-64. 2007
    ..4.24.27] is a thermostable neutral zinc metalloproteinase originally identified in the culture broth of Bacillus thermoproteolyticus Rokko...
  19. ncbi Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization
    Kuniyo Inouye
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Protein Expr Purif 46:248-55. 2006
    Thermolysin is a representative zinc metalloproteinase derived from Bacillus thermoproteolyticus and a target in protein engineering to understand the catalytic mechanism and thermostability...
  20. ncbi Identification of variant molecules of Bacillus thermoproteolyticus ferredoxin: crystal structure reveals bound coenzyme A and an unexpected [3Fe-4S] cluster associated with a canonical [4Fe-4S] ligand motif
    Tadayoshi Shirakawa
    Department of Biology, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043, Japan
    Biochemistry 44:12402-10. 2005
    During the purification of recombinant Bacillus thermoproteolyticus ferredoxin (BtFd) from Escherichia coli, we have noted that some Fe-S proteins were produced in relatively small amounts compared to the originally identified BtFd ..
  21. ncbi Structural comparison suggests that thermolysin and related neutral proteases undergo hinge-bending motion during catalysis
    D R Holland
    Institute of Molecular Biology, University of Oregon, Eugene 97403
    Biochemistry 31:11310-6. 1992
    ..Crystal structures are known for three members of the bacterial neutral protease family: thermolysin from Bacillus thermoproteolyticus (TLN), the neutral protease from Bacillus cereus (NEU), and the elastase of Pseudomonas aeruginosa (PAE)..
  22. pmc Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene
    R A Bever
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, New York 14642
    J Bacteriol 170:4309-14. 1988
    ..Three zinc ligands and an active site were predicted for the mature elastase on the basis of its amino acid sequence homology with Bacillus thermoproteolyticus thermolysin.
  23. ncbi Purification, characterization cloning, and sequencing of metalloendopeptidase from Streptomyces septatus TH-2
    Tadashi Hatanaka
    Research Institute for Biological Sciences RIBS, Okayama, 7549 1 Kibichuo cho, Kaga gun, Okayama 716 1241, Japan
    Arch Biochem Biophys 434:289-98. 2005
    ..The primary structure of the enzyme showed 40 and 69% identities with that of thermolysin from Bacillus thermoproteolyticus and that of a metalloendopeptidase from Streptomyces griseus, respectively.
  24. ncbi Tertiary structure of Bacillus thermoproteolyticus [4Fe-4S] ferredoxin. Evolutionary implications for bacterial ferredoxins
    K Fukuyama
    Faculty of Engineering, Tottori University, Japan
    J Mol Biol 199:183-93. 1988
    The structure of a low-potential [4Fe-4S] ferredoxin from Bacillus thermoproteolyticus has been solved using anomalous scattering data from iron atoms in the diffraction data of native crystals and refined partially to a crystallographic ..
  25. ncbi The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate
    Jean François Gaucher
    Laboratoire de Cristallographie et RMN Biologiques UMR 8015, CNRS, Universite Paris V, Faculte de Pharmacie, 4 avenue de l Observatoire, 75270 Paris Cedex 06, France
    Acta Crystallogr D Biol Crystallogr 58:2198-200. 2002
    A new crystallization protocol for thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus is presented...
  26. ncbi Atomic resolution structures of oxidized [4Fe-4S] ferredoxin from Bacillus thermoproteolyticus in two crystal forms: systematic distortion of [4Fe-4S] cluster in the protein
    Keiichi Fukuyama
    Department of Biology Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka, 560 0043, Japan
    J Mol Biol 315:1155-66. 2002
    Diffraction data of two crystal forms (forms I and II) of [4Fe-4S] ferredoxin from Bacillus thermoproteolyticus have been collected to 0.92 A and 1.00 A resolutions, respectively, at 100 K using synchrotron radiation...
  27. ncbi Amino-acid sequence and three-dimensional structure of the Staphylococcus aureus metalloproteinase at 1.72 A resolution
    A Banbula
    Jagiellonian University Institute of Molecular Biology Al Mickiewicza 3, 31 120, Krakow, Poland
    Structure 6:1185-93. 1998
    ..This enzyme exhibits in vitro activity against several molecules of biological significance for the host, indicating that it is involved in the pathology of staphylococcal diseases...

Patents1

  1. Protease glue from Bacillus thermoproteolyticus rokko, procedure for obtaining it and its use
    Patent Number: EP0418625-A1; Date:1991-03-27