Lactococcus lactis subsp. cremoris MG1363

Summary

Alias: Lactococcus lactis subsp. cremoris str. MG1363, Lactococcus lactis subsp. cremoris strain MG1363

Top Publications

  1. pmc Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363
    Aldert L Zomer
    Department of Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, 9750 AA Haren, The Netherlands
    J Bacteriol 189:1366-81. 2007
  2. pmc Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA
    Laurence Serre
    Institut de Biologie Structurale, CNRS CEA, 41 av Jules Horowitz, 38027 Grenoble Cedex 01, France
    EMBO J 21:2854-65. 2002
  3. ncbi Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase
    Franck Coste
    Centre de Biophysique Moleculaire, UPR4301, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Biol Chem 279:44074-83. 2004
  4. ncbi Six putative two-component regulatory systems isolated from Lactococcus lactis subsp. cremoris MG1363
    M O'Connell-Motherway
    Department of Microbiology and National Food Biotechnology Centre, National University of Ireland, Cork
    Microbiology 146:935-47. 2000
  5. ncbi The Lactococcus lactis sex-factor aggregation gene cluA
    J J Godon
    AFRC Institute of Food Research, Norwich, UK
    Mol Microbiol 12:655-63. 1994
  6. ncbi Splicing of a group II intron in a functional transfer gene of Lactococcus lactis
    C Shearman
    Institute of Food Research, Colney, Norwich, UK
    Mol Microbiol 21:45-53. 1996
  7. ncbi Identification and characterisation of a gene encoding aminoacylase activity from Lactococcus lactis MG1363
    P Curley
    Department of Microbiology, University College, Western Rd, Cork, Ireland
    FEMS Microbiol Lett 183:177-82. 2000
  8. pmc Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer
    M Kilstrup
    Department of Microbiology, Technical University of Denmark, Building 301, DK2800 Lyngby, Denmark
    Nucleic Acids Res 28:E55. 2000
  9. pmc The membrane-bound H(+)-ATPase complex is essential for growth of Lactococcus lactis
    B J Koebmann
    Department of Microbiology, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Bacteriol 182:4738-43. 2000
  10. pmc The pyrimidine operon pyrRPB-carA from Lactococcus lactis
    J Martinussen
    Department of Microbiology, The Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Bacteriol 183:2785-94. 2001

Patents

  1. PROCESS FOR THE LYSIS OF A CULTURE OF LACTIC ACID BACTERIA BY MEANS OF A LYSIN, AND USES OF THE RESULTING LYSED CULTURE
  2. METALLOPORPHYRIN INDUCIBLE PROMOTER
  3. Metalloporphyrin inducible promoter
  4. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
  5. Low temperature natural cheese and production method thereof

Scientific Experts

  • K F Jensen
  • Pascal Rigolet
  • J Martinussen
  • P Hols
  • M Kilstrup
  • J W Sanders
  • P Duwat
  • J Arnau
  • M H N Hoefnagel
  • H Ingmer
  • Arsalan Haseeb Zaidi
  • Patrick Veiga
  • Christian Bille Jendresen
  • William M Smith
  • Daniel Llull
  • Karine Pereira de Jésus
  • Claudia A McDonald
  • Wilbert Sybesma
  • Charles Zelwer
  • Bertrand Castaing
  • H G Griffin
  • Arnold J M Driessen
  • Laurence Serre
  • Patrick J Bakkes
  • M J Gasson
  • Nobuya Inagaki
  • Clara Roces
  • Bénédicte Cesselin
  • P Rowland
  • Kalpana Sriraman
  • Oscar P Kuipers
  • Ramasubramanian Sundaramoorthy
  • Aldert L Zomer
  • Magdalena Kowalczyk
  • Timothy J Stillman
  • Sebastian M J Klaus
  • Jeroen Hugenholtz
  • Franck Coste
  • F S Nielsen
  • Sofie Nørager
  • Serge Boiteux
  • P S Andersen
  • Scott A Beatson
  • Junchao Dou
  • S Larsen
  • Mark S Turner
  • Aijaz H Soomro
  • O Bjornberg
  • Gary A Dykes
  • Thi Huong Pham
  • Lin Lei
  • Olivier Danot
  • Bruce A Palfey
  • Bastiaan P Krom
  • Eric Morello
  • Sandrine Blanié
  • Olivier Son
  • Isabelle Poquet
  • Helene Rogniaux
  • Henny C van der Mei
  • Julien Briffotaux
  • O P Kuipers
  • P Curley
  • B J Koebmann
  • N Dobric
  • M O'Connell-Motherway
  • D van Sinderen
  • M Fernandez
  • Akira Taketo
  • Elvis Bernard
  • Ken Ichi Kodaira
  • Meriem El Karoui
  • Michael Erkelenz
  • Ana B Campelo
  • Pascal Courtin
  • Yasushi Ono
  • Beatriz Martinez
  • Jean Jacques Gratadoux
  • Marie Pierre Chapot-Chartier
  • Ayanori Yamakawa
  • Joao P C Pinto
  • Akinori Iguchi
  • Ana Rodriguez
  • Takahiro Yokoyama
  • Alexandra Gruss
  • Philippe Gaudu
  • Saulius Kulakauskas
  • Djae Ali
  • Ken ji Yokoi
  • D O Gostick

Detail Information

Publications64

  1. pmc Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363
    Aldert L Zomer
    Department of Molecular Genetics, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, 9750 AA Haren, The Netherlands
    J Bacteriol 189:1366-81. 2007
    ....
  2. pmc Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA
    Laurence Serre
    Institut de Biologie Structurale, CNRS CEA, 41 av Jules Horowitz, 38027 Grenoble Cedex 01, France
    EMBO J 21:2854-65. 2002
    ..In addition, the modelling of the 8-oxoguanine residue allows us to define an enzyme pocket that may accommodate the extrahelical oxidized base...
  3. ncbi Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase
    Franck Coste
    Centre de Biophysique Moleculaire, UPR4301, CNRS, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Biol Chem 279:44074-83. 2004
    ..The significant differences between the Fpg recognition modes of 8-oxodG and FapydG provide new insights into the Fpg substrate specificity...
  4. ncbi Six putative two-component regulatory systems isolated from Lactococcus lactis subsp. cremoris MG1363
    M O'Connell-Motherway
    Department of Microbiology and National Food Biotechnology Centre, National University of Ireland, Cork
    Microbiology 146:935-47. 2000
    ..Mutational analysis of the remaining four systems revealed that they are implicated in susceptibility to extreme pH, osmotic or oxidative conditions, or the regulation of phosphatase activity in L. lactis...
  5. ncbi The Lactococcus lactis sex-factor aggregation gene cluA
    J J Godon
    AFRC Institute of Food Research, Norwich, UK
    Mol Microbiol 12:655-63. 1994
    ..Domains II and IV are conserved at the amino acid level and probably have common structural roles whereas domain III is variable and may control binding specificity...
  6. ncbi Splicing of a group II intron in a functional transfer gene of Lactococcus lactis
    C Shearman
    Institute of Food Research, Colney, Norwich, UK
    Mol Microbiol 21:45-53. 1996
    ..Here we report the characterization of a new group II intron in the Gram-positive bacterium L. lactis and demonstrate for the first time in bacteria both splicing in vivo and an active role for the gene carrying the intron...
  7. ncbi Identification and characterisation of a gene encoding aminoacylase activity from Lactococcus lactis MG1363
    P Curley
    Department of Microbiology, University College, Western Rd, Cork, Ireland
    FEMS Microbiol Lett 183:177-82. 2000
    ..The amd1 gene was overexpressed in L. lactis NZ9800 under the control of the inducible nisA promoter and the deacetylating capacity of its gene product was measured on a number of substrates...
  8. pmc Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer
    M Kilstrup
    Department of Microbiology, Technical University of Denmark, Building 301, DK2800 Lyngby, Denmark
    Nucleic Acids Res 28:E55. 2000
    ..Therefore, the presence of the vectorette mismatched region is not required when unphosphorylated cassettes are used. As an example we report the amplification of the era gene from Lactococcus lactis...
  9. pmc The membrane-bound H(+)-ATPase complex is essential for growth of Lactococcus lactis
    B J Koebmann
    Department of Microbiology, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Bacteriol 182:4738-43. 2000
    ..When grown on GM17 plates the resulting strain was completely dependent on the presence of nisin for growth. These data demonstrate that the H(+)-ATPase is essential for growth of L. lactis under these conditions...
  10. pmc The pyrimidine operon pyrRPB-carA from Lactococcus lactis
    J Martinussen
    Department of Microbiology, The Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Bacteriol 183:2785-94. 2001
    ..The expression of the pyrimidine biosynthetic genes including the pyrRPB-carA operon is subject to control at the transcriptional level, most probably by an attenuator mechanism in which PyrR acts as the regulatory protein...
  11. ncbi Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates
    Ramasubramanian Sundaramoorthy
    Division of Biological Chemistry and Molecular Microbiology, College of Life Sciences, University of Dundee, UK
    FEBS J 274:275-86. 2007
    ..LlPDH can now serve as a model system for structure-based inhibitor design targeting the enzyme from Trypanosoma species...
  12. ncbi The structural basis for catalysis and specificity of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis
    Pascal Rigolet
    L U R E Centre Universitaire Paris Sud, Bât 209D BP 34, F 91898 Orsay Cedex, France
    Structure 10:1383-94. 2002
    ..A C-terminal moiety probably plays a role in the tropism of X-PDAP toward the cellular membrane. These results give new insights for further exploration of the role of the enzymes of the SC clan...
  13. ncbi Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function
    Sofie Nørager
    Centre for Crystallographic Studies, University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen, Denmark
    J Biol Chem 278:28812-22. 2003
    ..Furthermore, in one mutant structure we observed differences between the two monomers of the dimer, confirming an apparent asymmetry between the two substrate binding sites that was indicated by the kinetic results...
  14. pmc Increased production of folate by metabolic engineering of Lactococcus lactis
    Wilbert Sybesma
    Wageningen Centre for Food Sciences, NIZO Food Research, 6718 ZB Ede, The Netherlands
    Appl Environ Microbiol 69:3069-76. 2003
    ..The cloning and overexpression of folA, encoding dihydrofolate reductase, decreased the folate production twofold, suggesting a feedback inhibition of reduced folates on folate biosynthesis...
  15. ncbi Cloning, DNA sequence analysis, and deletion of a gene encoding diacetyl-acetoin reductase from Lactococcus lactis
    P Aungpraphapornchai
    Institute of Food Research, Norwich Research Park, Colney, UK
    DNA Seq 10:163-72. 1999
    ..The dar gene has been deleted from the chromosome by double cross-over homologous recombination...
  16. ncbi A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants
    Sebastian M J Klaus
    Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
    J Biol Chem 280:5274-80. 2005
    ..and Kraszewska, E. (2002) J. Biol. Chem. 277, 50482-50486). However, we found that this activity is negligible at physiological levels of Mn(2+) and that, with 1 mM Mg(2+), AtNUDT1 prefers DHNTP and (deoxy) nucleoside triphosphates...
  17. ncbi The crystal structures of Lactococcus lactis MG1363 Dps proteins reveal the presence of an N-terminal helix that is required for DNA binding
    Timothy J Stillman
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, Firth Court, Western Bank, University of Sheffield, Sheffield S10 2TN, UK
    Mol Microbiol 57:1101-12. 2005
    ..coli, Bacillus brevis and Mycobacterium smegmatis Dps:DNA complexes, in which DNA interacts with crystalline Dps phases, L. lactis DNA:Dps complexes appeared as non-crystalline aggregates of protein and DNA in electron micrographs...
  18. ncbi Identification and characterization of a cystathionine beta/gamma-lyase from Lactococcus lactis ssp. cremoris MG1363
    N Dobric
    Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Vic, Australia
    FEMS Microbiol Lett 182:249-54. 2000
    ..cremoris MG1363, its overexpression in Escherichia coli and some functional characteristics of the purified recombinant protein...
  19. pmc Structural insights into abasic site for Fpg specific binding and catalysis: comparative high-resolution crystallographic studies of Fpg bound to various models of abasic site analogues-containing DNA
    Karine Pereira de Jésus
    Centre de Biophysique Moleculaire, CNRS rue Charles Sadron, 45071 Orleans Cedex 02, France
    Nucleic Acids Res 33:5936-44. 2005
    ....
  20. ncbi Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering
    P Hols
    Microbial Ingredients Section, NIZO Food Research, Ede, The Netherlands
    Nat Biotechnol 17:588-92. 1999
    ..Finally, stereospecific production (>99%) of L-alanine was achieved by disrupting the gene encoding alanine racemase, opening the door to the industrial production of this stereoisomer in food products or bioreactors...
  21. ncbi In vitro DNA binding of purified CcpA protein from Lactococcus lactis IL1403
    Magdalena Kowalczyk
    Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland
    Acta Biochim Pol 54:71-8. 2007
    ..Competition gel retardation assay performed on DNA sequences from two PEP:PTS regions demonstrated that the ybhE, bglS, rheB, yebE, ptcB and yecA genes situated in these regions are most probably directly regulated by CcpA...
  22. pmc The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis
    Arsalan Haseeb Zaidi
    Department of Molecular Microbiology, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
    J Bacteriol 190:7357-66. 2008
    ..Together, these data suggest a central role for an efflux-based mechanism in bile acid resistance and implicate LmrCD as the main system responsible in L. lactis...
  23. pmc HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis
    Kalpana Sriraman
    Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India
    Appl Environ Microbiol 74:7442-6. 2008
    ..lactis. Instead of using an L. lactis htrA mutant, the reduction of the HtrA level in wild-type recombinant cultures of L. lactis by ATR suppression may serve as a better strategy for the production of secreted recombinant proteins...
  24. pmc Identification of the asparagine synthase responsible for D-Asp amidation in the Lactococcus lactis peptidoglycan interpeptide crossbridge
    Patrick Veiga
    INRA, UR888 Bactéries Lactiques et Pathogènes Opportunistes, F 78350 Jouy en Josas, France
    J Bacteriol 191:3752-7. 2009
    ..The level of D-Asp amidation in peptidoglycan has a strong effect on the sensitivity of bacteria to endogenous autolysins and to the cationic antimicrobials nisin and lysozyme...
  25. doi Inactivation of the Lactococcus lactis high-affinity phosphate transporter confers oxygen and thiol resistance and alters metal homeostasis
    Bénédicte Cesselin
    INRA, UR 888 Unité des Bactéries Lactiques et Pathogènes Opportunistes UBLO, F 78350 Jouy en Josas, France
    Microbiology 155:2274-81. 2009
    ..This study establishes a role for the pst locus in metal homeostasis, suggesting that pst inactivation lowers intracellular reactivity of copper and zinc, which would limit bacterial sensitivity to oxygen...
  26. doi Contribution of the CesR-regulated genes llmg0169 and llmg2164-2163 to Lactococcus lactis fitness
    Clara Roces
    Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lacteos de Asturias IPLA CSIC, Asturias, Spain
    Int J Food Microbiol 133:279-85. 2009
    ..lactis under technologically-relevant stresses and their functionality could be a useful marker to select robust dairy starters...
  27. doi Molecular properties of the glucosaminidase AcmA from Lactococcus lactis MG1363: mutational and biochemical analyses
    Nobuya Inagaki
    Molecular Biology Group, Graduate School of Science and Engineering, University of Toyama, Gofuku, Toyama, Japan
    Gene 447:61-71. 2009
    ..AcmA and Glu(atlwm) were purified from E. coli recombinant cells, and their enzymatic properties were studied...
  28. doi Two nucleoside transporters in Lactococcus lactis with different substrate specificities
    Jan Martinussen
    Center for Systems Microbiology, DTU Systems Biology, Building 301, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    Microbiology 156:3148-57. 2010
    ..The bmpA gene was separated from the nupABC operon by 60 kb. Moreover, bmpA was subject to regulation by purine availability, whereas the nupABC operon was constitutively expressed...
  29. pmc Lactococcus lactis ZitR is a zinc-responsive repressor active in the presence of low, nontoxic zinc concentrations in vivo
    Daniel Llull
    INRA, UMR1319 Micalis Microbiologie de l Alimentation au Service de la Santé, Bât 222, Domaine de Vilvert, F 78352 Jouy en Josas Cedex, France
    J Bacteriol 193:1919-29. 2011
    ..The characterization of a MarR family zinc-responsive repressor in this report gives insight into the way Streptococcaceae efficiently adapt to Zn(II) fluctuations in their diverse ecological niches...
  30. pmc Cholate-stimulated biofilm formation by Lactococcus lactis cells
    Arsalan Haseeb Zaidi
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, Nÿenborgh 7, 9747 AG Groningen, Netherlands
    Appl Environ Microbiol 77:2602-10. 2011
    ..These data suggest that non-transporter-based cholate resistance in L. lactis is due to alterations in the cell surface that stimulate cells to form resistant biofilms...
  31. pmc Substrate binding and reactivity are not linked: grafting a proton-transfer network into a Class 1A dihydroorotate dehydrogenase
    Claudia A McDonald
    Department of Biological Chemistry, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109 0606, United States
    Biochemistry 50:2714-6. 2011
    ..Our data show that residue 71 is an important determinant of ligand binding specificity. The Leu71Phe mutation tightens dihydrooroate binding but weakens the binding of benzoate inhibitors of Class 1A enzymes...
  32. doi The PurR regulon in Lactococcus lactis - transcriptional regulation of the purine nucleotide metabolism and translational machinery
    Christian Bille Jendresen
    Center for Systems Microbiology, Department for Systems Biology, Building 301, Technical University of Denmark, DK 2800 Kgs, Lyngby, Denmark
    Microbiology 158:2026-38. 2012
    ..Of special interest is the presence of PurBox motifs in rrn promoters, suggesting a novel connection between nucleotide availability and the translational machinery...
  33. pmc Heat resistance and salt hypersensitivity in Lactococcus lactis due to spontaneous mutation of llmg_1816 (gdpP) induced by high-temperature growth
    William M Smith
    School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland, Australia
    Appl Environ Microbiol 78:7753-9. 2012
    ..This study demonstrates that spontaneous mutations can occur during high-temperature growth of L. lactis and that inactivation of llmg_1816 leads to temperature resistance and salt hypersensitivity...
  34. ncbi The crystal structure of the flavin containing enzyme dihydroorotate dehydrogenase A from Lactococcus lactis
    P Rowland
    Centre for Crystallographic Studies, Department of Chemistry University of Copenhagen, Universitetsparken 5, DK 2100 Copenhagen O, Denmark
    Structure 5:239-52. 1997
    ..lactis. The differences between the two L. lactis enzymes make them particularly interesting for studying flavoprotein redox reactions and for identifying the differences between the enzyme families...
  35. pmc Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N
    I J van Alen-Boerrigter
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research, Ede
    Appl Environ Microbiol 57:2555-61. 1991
    ..The intracellular location of aminopeptidase N in L. lactis was confirmed by immunogold labeling of lactococcal cells...
  36. ncbi Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363
    M van Asseldonk
    Department of Biophysical Chemistry, Netherlands Institute for Dairy Research, NIZO, Ede
    Gene 95:155-60. 1990
    ..Usp45 has an unusual aa composition and distribution, and it is predicted to be structurally homologous with P54 of Enterococcus faecium. Up to now, no biological activity could be postulated for this secreted protein...
  37. ncbi Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector
    F Dickely
    Department of Genetics, Christian Hansen s Laboratorium Danmark A S, Hørsholm
    Mol Microbiol 15:839-47. 1995
    ..The pepN gene from L. lactis Wg2 was subcloned resulting in a food-grade plasmid giving a four- to fivefold increase in lysine aminopeptidase activity...
  38. pmc Stress response in Lactococcus lactis: cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene
    J W Sanders
    Department of Genetics, University of Groningen, NN Haren, The Netherlands
    J Bacteriol 177:5254-60. 1995
    ..The growth rate of an insertion mutant of sodA did not differ from that of the wild type in standing cultures but was decreased in aerated cultures...
  39. ncbi Genetic aspects of aromatic amino acid biosynthesis in Lactococcus lactis
    H G Griffin
    Institute of Food Research, Norwich Research Park, Colney, UK
    Mol Gen Genet 246:119-27. 1995
    ..The aroA gene from L. lactis has been shown to complement an E. coli mutant strain deficient in this gene. The arrangement of genes involved in aromatic amino acid biosynthesis in L. lactis appears to differ from that in other organisms...
  40. pmc Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis
    J Martinussen
    Center for Lactic Acid Bacteria, Laboratory of Microbiology, Technical University of Denmark, Lyngby
    J Bacteriol 176:6457-63. 1994
    ..Secondary mutants in thymidine phosphorylase and thymidine kinase were isolated by selection for resistance to high concentrations of 5-fluorouracil...
  41. pmc Two different dihydroorotate dehydrogenases in Lactococcus lactis
    P S Andersen
    Department of Microbiology, Technical University of Denmark, Lyngby
    J Bacteriol 176:3975-82. 1994
    ..We constructed mutants containing a mutated form of either one or the other or both of the pyrD genes. Only the double mutant is pyrimidine auxotrophic...
  42. ncbi Cloning and sequence analysis of the dnaK gene region of Lactococcus lactis subsp. lactis
    T Eaton
    AFRC Institute of Food Research, Norwich Research Park, Colney, UK
    J Gen Microbiol 139:3253-64. 1993
    ..These repeats are thought to be involved in regulation of the heat-shock genes. The DnaK homologue is induced approximately 3-fold on heat shock at 42 degrees C...
  43. pmc Tripeptidase gene (pepT) of Lactococcus lactis: molecular cloning and nucleotide sequencing of pepT and construction of a chromosomal deletion mutant
    I Mierau
    Department of Genetics, University of Groningen, Haren, The Netherlands
    J Bacteriol 176:2854-61. 1994
    ..lactis was constructed. No differences between the growth of the mutant and that of the wild-type strain in milk or in chemically defined medium with casein as the sole source of essential amino acids were observed...
  44. ncbi A non-essential glutamyl aminopeptidase is required for optimal growth of Lactococcus lactis MG1363 in milk
    K J l'Anson
    Institute of Food Research, Norwich Laboratory, Colney, UK
    Microbiology 141:2873-81. 1995
    ..Mutants of L. lactis in which the pepA gene was inactivated grew to normal cell densities in milk but exhibited a reduced growth rate during the exponential phase. Thus whilst PepA is required for optimal growth it is not essential...
  45. ncbi The ribonucleotide reductase system of Lactococcus lactis. Characterization of an NrdEF enzyme and a new electron transport protein
    A Jordan
    Department of Biochemistry 1, Medical Nobel Institute, MBB, Karolinska Institutet, S 17177 Stockholm, Sweden
    J Biol Chem 271:8779-85. 1996
    ..The NrdEF enzymes use NrdH proteins as electron transporter in place of thioredoxin or glutaredoxin used by NrdAB enzymes. The two classes also differ in their allosteric regulation by dATP...
  46. pmc Sequence analysis and identification of the pyrKDbF operon from Lactococcus lactis including a novel gene, pyrK, involved in pyrimidine biosynthesis
    P S Andersen
    Department of Microbiology, Technical University of Denmark, Lyngby, Denmark
    J Bacteriol 178:5005-12. 1996
    ..Three of these were excluded as members of the pyr operon by insertional analysis (orfA) or by their opposite direction of transcription (orfE and gidB). orfC, however, seems to be the distal gene in the pyrKDbF-orfC operon...
  47. pmc Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis
    S R Swindell
    Institute of Food Research, Colney, United Kingdom
    Appl Environ Microbiol 62:2641-3. 1996
    ..Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl...
  48. pmc Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1
    H W van Veen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Proc Natl Acad Sci U S A 93:10668-72. 1996
    ..As LmrA can be functionally expressed in E. coli, it offers a useful prokaryotic model for future studies on the molecular mechanism of MDR1-like multidrug transporters...
  49. ncbi The B form of dihydroorotate dehydrogenase from Lactococcus lactis consists of two different subunits, encoded by the pyrDb and pyrK genes, and contains FMN, FAD, and [FeS] redox centers
    F S Nielsen
    Center for Enzyme Research, Institute of Molecular Biology, University of Copenhagen, Solvgade 83H, DK 1307 Copenhagen K, Denmark
    J Biol Chem 271:29359-65. 1996
    ..Based on these results and on analysis of published sequences, we propose that the architecture of the deltakappa-enzyme is representative for the dihydroorotate dehydrogenases from Gram-positive bacteria...
  50. pmc Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase
    M Fernandez
    Microbial Ingredients Section, NIZO Food Research, Ede, The Netherlands
    Appl Environ Microbiol 66:42-8. 2000
    ....
  51. pmc Characterization of Lactococcus lactis UV-sensitive mutants obtained by ISS1 transposition
    P Duwat
    Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 179:4473-9. 1997
    ....
  52. pmc Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase
    J Arnau
    Biotechnological Institute, Hørsholm, Denmark
    J Bacteriol 179:5884-91. 1997
    ..lactis pfl strains were unable to produce formate under anaerobic growth. Higher levels of diacetyl and acetoin were produced anaerobically in the constructed Lactococcus lactis subsp. lactis biovar diacetylactis pfl strain...
  53. ncbi Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis
    O Bjornberg
    Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Biochemistry 36:16197-205. 1997
    ..The possible acid-base chemistry of Cys 130 is compared to previous work on mammalian dihydropyrimidine dehydrogenases, flavoenzymes, which catalyze the reversed reaction, namely the reduction of pyrimidine bases...
  54. pmc Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence
    M El Karoui
    Institut National de la Recherche Agronomique, Jouy en Josas, France
    Proc Natl Acad Sci U S A 95:626-31. 1998
    ..We speculate that exonuclease/recombinase enzymes whose activities are modulated by short DNA sequences are widespread among bacteria...
  55. ncbi Identification of a sodium chloride-regulated promoter in Lactococcus lactis by single-copy chromosomal fusion with a reporter gene
    J W Sanders
    Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Mol Gen Genet 257:681-5. 1998
    ..Screening of integrants in the presence of 0.3 M NaCl resulted in the isolation of a clone (NS3) in which expression of lacZ was dependent on the concentration of chloride ions...
  56. pmc The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function
    P Rowland
    Centre for Crystallographic Studies, Department of Chemistry, University of Copenhagen, Denmark
    Protein Sci 7:1269-79. 1998
    ....
  57. pmc The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically
    J Martinussen
    Department of Microbiology, The Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Bacteriol 180:4380-6. 1998
    ..Upstream of the carB gene, an open reading frame showing a high degree of similarity to those of glutathione peroxidases from other organisms was identified...
  58. pmc A natural large chromosomal inversion in Lactococcus lactis is mediated by homologous recombination between two insertion sequences
    M L Daveran-Mingot
    Laboratoire de Microbiologie et Genetique Moleculaire du CNRS, Universite Paul Sabatier, 31062 Toulouse, France
    J Bacteriol 180:4834-42. 1998
    ..The large inversion presumably conferred no selective disadvantage on strain NCDO763 because this rearrangement did not alter the oriC-terC symmetry of the chromosome and the local genetic environment...
  59. pmc 6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme
    E Tetaud
    Laboratoire de Biologie Moléculaire et Immunologie de Parasites Protozoaires, UPRESA 5016 CNRS, Universite Bordeaux II, France
    Biochem J 338:55-60. 1999
    ..A second 6-PGDH gene has been identified from the genome of B. subtilis. This second isoform contains an arginine (Arg-34) in this position, suggesting that B. subtilis has two 6-PGDHs with different coenzyme specificities...
  60. ncbi ClpP participates in the degradation of misfolded protein in Lactococcus lactis
    D Frees
    Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark
    Mol Microbiol 31:79-87. 1999
    ..Thus, our data suggest that ClpP plays a major role in the degradation of misfolded proteins...
  61. ncbi Effects of metabolic flux on stress response pathways in Lactococcus lactis
    P Duwat
    Génétique Appliquée, URLGA, Institut National de la Recherche Agronomique, Jouy en Josas, France
    Mol Microbiol 31:845-58. 1999
    ..Our results reveal that cellular metabolic pathways are intimately related to stress response and that the flux of particular metabolites, notably guanine and phosphate, may be implicated in stress response in lactococci...
  62. pmc Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria
    H Ingmer
    Centre for Advanced Food Studies, Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, DK 1958 Frederiksberg C
    J Bacteriol 181:2075-83. 1999
    ..Thus, our data suggest that ClpE, along with ClpP, which recently was shown to participate in the degradation of randomly folded proteins in L. lactis, could be necessary for degrading proteins generated by certain types of stress...
  63. ncbi Two operons that encode FNR-like proteins in Lactococcus lactis
    D O Gostick
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 31:1523-35. 1999
    ..It was concluded tentatively that FlpA and FlpB regulate overlapping modulons, including systems concerned with zinc uptake, in response to metal ion or oxidative stress...
  64. ncbi Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N
    P S Tan
    Department of Microbiology, University of Groningen, Haren, The Netherlands
    FEBS Lett 306:9-16. 1992
    ..A zinc-binding site, as well as the catalytic site for PepN, is predicted to lie within this conserved stretch. Putative promoter regions upstream of PepN were confirmed by primer extension analysis...

Patents5

  1. PROCESS FOR THE LYSIS OF A CULTURE OF LACTIC ACID BACTERIA BY MEANS OF A LYSIN, AND USES OF THE RESULTING LYSED CULTURE
    Patent Number: WO9531561-A1; Date:1995-11-23
  2. METALLOPORPHYRIN INDUCIBLE PROMOTER
    Patent Number: WO2012093375-A1; Date:2012-07-12
  3. Metalloporphyrin inducible promoter
    Patent Number: EP2474618-A1; Date:2012-07-11
  4. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
    Patent Number: KR1020130117753-A; Date:2013-10-28
  5. Low temperature natural cheese and production method thereof
    Patent Number: JP2010207129-A; Date:2010-09-24