Genomes and Genes
Staphylococcus aureus subsp. aureus NCTC 8325
Alias: Staphylococcus aureus NCTC 8325, Staphylococcus aureus subsp. aureus str. NCTC 8325, Staphylococcus aureus subsp. aureus strain NCTC 8325
Publications127 found, 100 shown here
- Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptidesC Signas
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala
Proc Natl Acad Sci U S A 86:699-703. 1989..All three peptides interacted with fibronectin, as indicated by their ability to inhibit binding of fibronectin to staphylococcal cells, whereas an unrelated 37-amino acid peptide showed no inhibitory activity...
- Identification and nucleotide sequence of the delta-lysin gene, hld, adjacent to the accessory gene regulator (agr) of Staphylococcus aureusL Janzon
Department of Bacteriology, Karolinska Institutet, Stockholm, Sweden
Mol Gen Genet 219:480-5. 1989..Determination of hld mRNA half-life in different growth phases indicated that this regulation is at the level of transcription...
- Characterization of a Tn551-mutant of Staphylococcus aureus defective in the production of several exoproteinsA T Giraudo
Departamento de Microbiologia, Facultad de Ciencias Exactas, Fisico Quimicas y Naturales, Universidad Nacional Río Cuarto, Cordoba, Argentina
Can J Microbiol 40:677-81. 1994..The phenotype of the mutant was different from that of other insertional mutants affecting exoprotein synthesis, such as agr, xpr, or sar. This new mutation has been designated sae (for S. aureus exoprotein expression)...
- Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureusB Wieland
Mikrobielle Genetik, Universitat Tubingen, Federal Republic of Germany
J Bacteriol 176:7719-26. 1994..Dehydrosqualene (4,4'-diapophytoene) is successively dehydrogenated by a desaturase (CrtN) to form the yellow main intermediate 4,4'-diaponeurosporene...
- Site-directed mutagenesis of the alpha-toxin gene of Staphylococcus aureus: role of histidines in toxin activity in vitro and in a murine modelB E Menzies
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
Infect Immun 62:1843-7. 1994....
- Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureusD McDevitt
Microbiology Department, Moyne Institute, Trinity College, Dublin, Ireland
Mol Microbiol 11:237-48. 1994..Significant homology was found between the ClfA protein and the fibronectin-binding proteins of S. aureus, particularly in the N- and C-termini...
- Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureusE W Brunskill
Program in Molecular and Cell Biology, University of Maryland, Baltimore County 21228, USA
J Bacteriol 178:611-8. 1996..These data suggest that the lytS and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein hydrolase activity associated with the cell...
- Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environmentsS N Coulter
PathoGenesis Corporation, 201 Elliott Avenue West, Seattle, WA 98119, USA
Mol Microbiol 30:393-404. 1998....
- Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptidesA Peschel
Mikrobielle Genetik, Universitat Tubingen, Waldhäuser Strasse 70 8, 72076 Tubingen, Germany
J Biol Chem 274:8405-10. 1999..We propose a role of the D-alanine-esterified teichoic acids which occur in many pathogenic bacteria in the protection against human and animal defense systems...
- The essential Staphylococcus aureus gene fmhB is involved in the first step of peptidoglycan pentaglycine interpeptide formationS Rohrer
Institute of Medical Microbiology, University of Zurich, Gloriastr 32, Postfach, CH 8028 Zurich, Switzerland
Proc Natl Acad Sci U S A 96:9351-6. 1999..Because of its key role FmhB is a potential target for novel antibacterial agents that could control the threat of emerging multiresistant S. aureus...
- The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formationS E Cramton
Mikrobielle Genetik, Universitat Tubingen, D 72076 Tubingen, Germany
Infect Immun 67:5427-33. 1999..Cross-species hybridization experiments revealed the presence of icaA in several other Staphylococcus species, suggesting that cell-cell adhesion and the potential to form biofilms is conserved within this genus...
- Interactive regulatory pathways control virulence determinant production and stability in response to environmental conditions in Staphylococcus aureusJ A Lindsay
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK
Mol Gen Genet 262:323-31. 1999..How environmental signals are transduced to control alpha-haemolysin and serine protease production, activity and stability at multiple levels are discussed...
- The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogenE R Wann
Center for Extracellular Matrix Biology, Department of Biochemistry and Biophysics, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, Texas 77030 3303, USA
J Biol Chem 275:13863-71. 2000..Finally, by overexpressing FnbpA in a mutant strain of S. aureus that lacks the expression of both ClfA and ClfB, we show that native FnbpA can mediate the interaction of S. aureus with soluble Fg...
- Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureusP J McNamara
Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
J Bacteriol 182:3197-203. 2000..We named this ORF rot (for repressor of toxins) (GenBank accession no. AF189239) because of the activity associated with rot::Tn917 mutant strains...
- Exfoliatin-producing strains define a fourth agr specificity group in Staphylococcus aureusS Jarraud
Faculte de Medecine Laennec, 69372 Lyon Cedex 08, France
J Bacteriol 182:6517-22. 2000..This finding raises the question of the biological significance of the agr autoinduction threshold...
- Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysineA Peschel
Microbial Genetics, University of Tuebingen, 72076 Tuebingen, Germany
J Exp Med 193:1067-76. 2001..MprF thus constitutes a novel virulence factor, which may be of general relevance for bacterial pathogens and represents a new target for attacking multidrug resistant bacteria...
- PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in Staphylococcus aureusM J Horsburgh
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, England
Infect Immun 69:3744-54. 2001..However, it differs in its response to the metal balance within the cell and has the added capability of regulating iron uptake and storage...
- SarT, a repressor of alpha-hemolysin in Staphylococcus aureusK A Schmidt
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 13755, USA
Infect Immun 69:4749-58. 2001..Collectively, these results indicated that the sarA locus, contrary to the regulatory action of agr, induced alpha-hemolysin production by repressing sarT, a repressor of hla transcription...
- The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureusB Fournier
Unité de Biochimie Microbienne, URA 2172 du Centre National de la Recherche Scientifique, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
Mol Microbiol 41:247-61. 2001..Finally, arl was not autoregulated, but its expression was stimulated by agr and sarA. These results suggest that the Arl system interacts with both agr and sarA regulatory loci to modulate the virulence regulation network...
- Reassessing the role of Staphylococcus aureus clumping factor and fibronectin-binding protein by expression in Lactococcus lactisY A Que
Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
Infect Immun 69:6296-302. 2001..Taken together, the present observations suggest that if antiadhesin therapy were to be developed, at least both of the clfA and fnbA products should be blocked for the therapy to be effective...
- Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA lociP M Dunman
Infectious Diseases, Wyeth Ayerst Research, Pearl River, New York 10965, USA
J Bacteriol 183:7341-53. 2001....
- Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cellsR C Massey
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
Cell Microbiol 3:839-51. 2001..These findings may be of relevance to the development of preventive measures against systemic infection, and bacterial spread in the bacteraemic patient...
- Strain-dependent differences in the regulatory roles of sarA and agr in Staphylococcus aureusJon S Blevins
Department of Microbiology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
Infect Immun 70:470-80. 2002..Taken together, these results suggest that studies defining the regulatory roles of sarA and agr by using RN6390 are not always representative of the events that occur in clinical isolates of S. aureus...
- MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptakeMalcolm J Horsburgh
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
Mol Microbiol 44:1269-86. 2002..The co-ordinated regulation of metal ion homeostasis and oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed...
- Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonizationLouise M O'Brien
Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College Dublin, Dublin 2, Ireland
Cell Microbiol 4:759-70. 2002..We also showed that ClfB is transcribed by S. aureus in the human nares. We propose that ClfB is a major determinant in S. aureus nasal colonization...
- Global regulation of Staphylococcus aureus genes by RotB Said-Salim
Public Health Research Institute at the International Center of Public Health, Newark, New Jersey 07103, USA
J Bacteriol 185:610-9. 2003..aureus genes. Our data also indicate that Rot and agr have opposing effects on select target genes. These results provide further insight into the role of Rot in the regulatory cascade of S. aureus virulence gene expression...
- Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall-active antibiotics oxacillin and vancomycinSusan Boyle-Vavra
Department of Pediatrics, The University of Chicago, Illinois 60637, USA
Antimicrob Agents Chemother 47:1028-36. 2003..These data support the idea that pbpB expression is modulated by a trans-acting factor in response to the presence of the cell wall-active antibiotics vancomycin and oxacillin...
- Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequencesFiona M Roche
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
Microbiology 149:643-54. 2003..Several IgG samples purified from patients recovering from S. aureus infections had higher titres against Sas proteins than control IgG, suggesting that expression occurred during infection in some patients...
- Identification of genes controlled by the essential YycG/YycF two-component system of Staphylococcus aureusSarah Dubrac
Unité de Biochimie Microbienne, CNRS URA 2172, Institut Pasteur, 75724 Paris Cedex 15, France
J Bacteriol 186:1175-81. 2004....
- MprF-mediated biosynthesis of lysylphosphatidylglycerol, an important determinant in staphylococcal defensin resistancePetra Staubitz
Cellular and Molecular Microbiology, Medical Microbiology Department, University of Tubingen, Elfriede Aulhorn Str 6, D 72076 Tubingen, Germany
FEMS Microbiol Lett 231:67-71. 2004..This study forms the basis for further detailed analyses of L-PG biosynthesis and its role in bacterial infections...
- IsdA of Staphylococcus aureus is a broad spectrum, iron-regulated adhesinSimon R Clarke
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
Mol Microbiol 51:1509-19. 2004..Thus for S. aureus, iron is an important marker for the host environment, to which the bacterium responds by differential regulation of at least one element of its adhesive strategy...
- The N-terminal A domain of fibronectin-binding proteins A and B promotes adhesion of Staphylococcus aureus to elastinFiona M Roche
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
J Biol Chem 279:38433-40. 2004..This interaction was inhibited by soluble elastin peptides, suggesting a specific receptor-ligand interaction...
- Clumping factor B, a fibrinogen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) adhesin of Staphylococcus aureus, also binds to the tail region of type I cytokeratin 10Evelyn J Walsh
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland
J Biol Chem 279:50691-9. 2004..We provide an explanation for the molecular basis for S. aureus adherence to the squamous epithelium and suggest that nasal colonization might be prevented by reagents that inhibit this interaction...
- Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureusAgnieszka Bera
Microbial Genetics, University of Tubingen, D 72076 Tubingen, Auf der Morgenstelle, Germany
Mol Microbiol 55:778-87. 2005..We identified the first bacterial peptidoglycan-specific O-acetyltransferase in S. aureus and showed that OatA, an integral membrane protein, is the molecular basis for the high lysozyme resistance in staphylococci...
- Identification of an essential glycoprotease in Staphylococcus aureusLi Zheng
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, St Paul, MN 55108, USA
FEMS Microbiol Lett 245:279-85. 2005..Our results indicate that Gcp is a potential novel target for the development of antimicrobials against S. aureus infection...
- The multifunctional Staphylococcus aureus autolysin aaa mediates adherence to immobilized fibrinogen and fibronectinChristine Heilmann
Institute of Medical Microbiology, University of Munster, Domagkstr 10, D 48149 Munster, Germany
Infect Immun 73:4793-802. 2005..Finally, an aaa knockout mutant showed reduced adherence to surface-adsorbed fibrinogen and fibronectin, strongly suggesting a role for Aaa in the colonization of host factor-coated polymer surfaces and/or host tissue...
- Immune evasion by a staphylococcal complement inhibitor that acts on C3 convertasesSuzan H M Rooijakkers
Eijkman Winkler Institute, University Medical Center Utrecht, G04 614, 3584 CX Utrecht, The Netherlands
Nat Immunol 6:920-7. 2005..As a highly active and small soluble protein that acts exclusively on surfaces, staphylococcal complement inhibitor may represent a promising anti-inflammatory molecule...
- Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensingNeil Doherty
Institute of Infections, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, Nottingham NG7 2RD, United Kingdom
J Bacteriol 188:2885-97. 2006..aureus RN6390B during intracellular growth in epithelial cells: the wild type and a luxS mutant showed very similar growth patterns after their internalization by MAC-T cells...
- Early expression of SCIN and CHIPS drives instant immune evasion by Staphylococcus aureusSuzan H M Rooijakkers
Eijkman Winkler Center, University Medical Center Utrecht, Utrecht, The Netherlands
Cell Microbiol 8:1282-93. 2006..aureus proteins early during growth. Because SCIN and CHIPS are both efficient modulators of neutrophil chemotaxis, phagocytosis and killing, their early expression is necessary for efficient modulation of the early immune response...
- Global regulatory impact of ClpP protease of Staphylococcus aureus on regulons involved in virulence, oxidative stress response, autolysis, and DNA repairAntje Michel
Institut für Molekulare Infektionsbiologie, Rontgenring 11, D 97070 Wurzburg, Germany
J Bacteriol 188:5783-96. 2006..Our results indicate a strong impact of ClpP proteolytic activity on virulence, stress response, and physiology in S. aureus...
- Catalase (KatA) and alkyl hydroperoxide reductase (AhpC) have compensatory roles in peroxide stress resistance and are required for survival, persistence, and nasal colonization in Staphylococcus aureusKate Cosgrove
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom
J Bacteriol 189:1025-35. 2007..Thus, oxidative-stress resistance is an important factor in the ability of S. aureus to persist in the hospital environment and so contribute to the spread of human disease...
- Interaction of the GraRS two-component system with the VraFG ABC transporter to support vancomycin-intermediate resistance in Staphylococcus aureusMichael Meehl
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA
Antimicrob Agents Chemother 51:2679-89. 2007..aureus phenotype and may hold clues to the selective pressures on staphylococci upon exposure to selective cationic peptide antibiotics used in clinical practice...
- Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteinsHeather M Baker
Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand
J Mol Biol 374:1298-308. 2007..Structural comparisons also showed that the Sia binding site in SSL5 contains a substructure that is shared by other Sia binding proteins from bacteria as well as viruses and represents a common binding motif...
- Characterization of the accessory Sec system of Staphylococcus aureusIan R Siboo
Division of Infectious Diseases, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA
J Bacteriol 190:6188-96. 2008..Thus, the accessory Sec system of S. aureus is required for the export of SraP, and it appears to be dedicated to the transport of this substrate exclusively...
- Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureusStefanie Donat
Universitat Wurzburg, Institut für Molekulare Infektionsbiologie, Wurzburg, Germany
J Bacteriol 191:4056-69. 2009..The results of this study strongly indicate that PknB has a role in regulation of purine biosynthesis, autolysis, and central metabolic processes in S. aureus...
- Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureusMark J J B Sibbald
Department of Medical Microbiology, University Medical Center Groningen and University of Groningen, Hanzeplein 1, P O Box 30001, 9700 RB Groningen, The Netherlands
J Bacteriol 192:3788-800. 2010..Additionally, the results suggest that SecY2 can interact with the Sec1 channel, which would be consistent with the presence of a single set of secE and secG genes in S. aureus...
- Absence of nucleoid occlusion effector Noc impairs formation of orthogonal FtsZ rings during Staphylococcus aureus cell divisionHelena Veiga
Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781 901 Oeiras, Portugal
Mol Microbiol 80:1366-80. 2011..This is achieved via the action of Noc which restricts the placement of the division septum to one of an infinite number of potential division planes that exist in S. aureus...
- Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complexChristelle M Roux
Department of Microbiology and Immunology, University of Rochester Medical Center, School of Medicine and Dentistry, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA
J Bacteriol 193:5520-6. 2011..Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms...
- New range of vectors with a stringent 5-fluoroorotic acid-based counterselection system for generating mutants by allelic replacement in Staphylococcus aureusPeter Redder
Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland
Appl Environ Microbiol 78:3846-54. 2012..Finally, as proof of concept, we present six deletions or modifications of components in the S. aureus degradosome as well as the operon containing the cshB DEAD box helicase...
- The CshA DEAD-box RNA helicase is important for quorum sensing control in Staphylococcus aureusStella Oun
Department of Microbiology and Molecular Medicine, CMU, Medical Faculty, University of Geneva, Geneve, Switzerland
RNA Biol 10:157-65. 2013....
- Identification of a novel gene cluster encoding staphylococcal exotoxin-like proteins: characterization of the prototypic gene and its protein product, SET1R J Williams
Cellular Microbiology Research Group, Division of Surgical Sciences, University College London, London, United Kingdom
Infect Immun 68:4407-15. 2000..The distribution of allelic variants of the set genes among strains of S. aureus may contribute to differences in the pathogenic potential of this bacterium...
- Loss of clumping factor B fibrinogen binding activity by Staphylococcus aureus involves cessation of transcription, shedding and cleavage by metalloproteaseF M McAleese
Microbiology Department, Moyne Institute for Preventive Medicine, Trinity College, Dublin 2, Ireland
J Biol Chem 276:29969-78. 2001..Cleavage was detected at two sites, one located between residues Ser(197) and Leu(198) and the other between Ala(199) and Val(200). The truncated form of ClfB did not bind fibrinogen...
- Dispersed mode of Staphylococcus aureus cell wall synthesis in the absence of the division machineryMariana G Pinho
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
Mol Microbiol 50:871-81. 2003..The results have implications for understanding the nature of S. aureus morphogenesis and for inhibitors of cell division proteins as drug targets...
- Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotypeLindsey N Shaw
Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Bldg, Athens, GA 30602, USA
J Bacteriol 187:1751-62. 2005..Seemingly, some of these proteins may play a role in protein secretion; hence, their proteolytic inactivation by SspB has pleiotropic effects on the SspC-deficient mutant...
- Investigations into sigmaB-modulated regulatory pathways governing extracellular virulence determinant production in Staphylococcus aureusLindsey N Shaw
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
J Bacteriol 188:6070-80. 2006..Thus, we propose that the loss of individual genes in this chromosomal hot spot region results in a destabilization of cellular harmony and disruption of the sigmaB regulatory cascade...
- Transcriptional profiling of target of RNAIII-activating protein, a master regulator of staphylococcal virulenceMoshe Korem
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel
Infect Immun 73:6220-8. 2005..TRAP is thus demonstrated to be a master regulator of staphylococcal pathogenesis...
- Molecular basis of resistance to muramidase and cationic antimicrobial peptide activity of lysozyme in staphylococciSilvia Herbert
Microbial Genetics Department, University of Tubingen, Tubingen, Germany
PLoS Pathog 3:e102. 2007..The two lysozyme activities act synergistically, as the oatA/dltA or oatA/graRS double mutants are much more susceptible to lysozyme than each of the single mutants...
- Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosusS B Halio
Department of Chemical Engineering, North Carolina State University, Raleigh, 27695 7905, USA
J Bacteriol 178:2605-12. 1996..identified only two similar amino acid sequences: an open reading frame of unknown function from Staphylococcus aureus NCTC 8325 (171 amino acid residues, 18...
- Quinolone resistance mediated by norA: physiologic characterization and relationship to flqB, a quinolone resistance locus on the Staphylococcus aureus chromosomeE Y Ng
Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston 02114 2696
Antimicrob Agents Chemother 38:1345-55. 1994..locus, flqB, linked to transposon insertion omega 1108 and fus on the SmaI D fragment of the Staphylococcus aureus NCTC 8325 chromosome, the same fragment that contains the norA gene. S...
- The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil)S D Cox
Centre for Biostructural and Biomolecular Research, University of Western Sydney, Hawkesbury, New South Wales, Western Australia
J Appl Microbiol 88:170-5. 2000..of action against the Gram-negative bacterium Escherichia coli AG100, the Gram-positive bacterium Staphylococcus aureus NCTC 8325, and the yeast Candida albicans has been investigated using a range of methods...
- The inability of a bacteriophage to infect Staphylococcus aureus does not prevent it from specifically delivering a photosensitizer to the bacterium enabling its lethal photosensitizationC K Hope
Division of Microbial Diseases, UCL Eastman Dental Institute, London, UK
J Antimicrob Chemother 64:59-61. 2009..In this study we have investigated whether a bacteriophage requires the capacity to infect the bacterium to enhance lethal photosensitization when linked to a photosensitizer...
- Efflux-mediated bis-indole resistance in Staphylococcus aureus reveals differential substrate specificities for MepA and MepRTIMOTHY J OPPERMAN
Microbiotix, Inc, Worcester, MA 01605 4332, United States
Bioorg Med Chem 18:2123-30. 2010..To study the mechanism of action of bis-indoles, selections for mutants of Staphylococcus aureus NCTC 8325 with reduced susceptibility to several chemically related bis-indoles were carried out using serial ..
- Staphylococcal peptidoglycan interpeptide bridge biosynthesis: a novel antistaphylococcal target?U Kopp
Bayer AG, Pharma Research Antiinfectives I, Wuppertal, Germany
Microb Drug Resist 2:29-41. 1996..The drastic loss of beta-lactam resistance after inactivation of FemA or partial impairment of FemX even beyond the level of the sensitive wild-type strains renders these proteins attractive antistaphylococcal targets...
- Coenzyme A-disulfide reductase from Staphylococcus aureus: evidence for asymmetric behavior on interaction with pyridine nucleotidesJ Luba
Department of Biochemistry, Wake Forest University Medical Center, Winston Salem, North Carolina 27157, USA
Biochemistry 38:2725-37. 1999..The EH2.NADPH/EH4.NADP+ complex which results is reoxidized quantitatively in titrations with CoASSCoA, supporting a possible role for the asymmetric reduced dimer in catalysis...
- SarA and not sigmaB is essential for biofilm development by Staphylococcus aureusJaione Valle
Instituto de Agrobiotecnología y Recursos Naturales and Dpto de Producción Agraria, Universidad Pública de Navarra CSIC, Pamplona 31006, Spain
Mol Microbiol 48:1075-87. 2003....
- Transcriptional activation of the Staphylococcus aureus putP gene by low-proline-high osmotic conditions and during infection of murine and human tissuesWilliam R Schwan
Department of Microbiology, University of Wisconsin La Crosse, 1725 State Street, La Crosse, WI 54601, USA
Infect Immun 74:399-409. 2006..Thus, putP is transcriptionally activated by a low-proline and high osmotic environment both in growth media and in murine or human clinical specimens...
- Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315T Ito
Department of Bacteriology, Juntendo University, Tokyo, Japan
Antimicrob Agents Chemother 43:1449-58. 1999..However, there was no ORF that might encode mec DNA-specific transposase or integrase proteins, indicating that the mec DNA is not a transposon or a bacteriophage in nature...
- Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activityB Walker
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
J Biol Chem 267:10902-9. 1992..The mutant polypeptide nevertheless binds tightly to erythrocytes as a monomer, strengthening the idea that oligomerization is required for cell lysis...
- The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutantsP Phonimdaeng
Department of Microbiology, Moyne Institute, Trinity College, Dublin, Ireland
Mol Microbiol 4:393-404. 1990..This contradicts findings of several groups using Coa- mutants generated by chemical mutagenesis and suggests that the earlier results were obtained with strains that had suffered additional mutations in virulence-related genes...
- Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coliH L Shuttleworth
Microbial Technology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U K
Gene 58:283-95. 1987..The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene...
- A genetic system for analysis of staphylococcal nucleaseD Shortle
Gene 22:181-9. 1983..In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme...
- Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46G S Gray
Infect Immun 46:615-8. 1984..The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions...
- Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureusL Ferrero
Departement des Biotechnologies, Rhone Poulenc Rorer S A, Vitry sur Seine, France
Antimicrob Agents Chemother 39:1554-8. 1995..Ferrero, B. Cameron, B. Manse, D. Lagneaux, J. Crouzet, A. Famechon, and F. Blanche, Mol. Microbiol. 13:641-653, 1994) that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus...
- A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureusL Armstrong-Buisseret
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK
Microbiology 141:1655-61. 1995..Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC...
- Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitorsP Staedtler
Arbeitsgruppe Physiologie der Mikroorganismen, Abteilung Biologie, Ruhr Universitat Bochum, Germany
Eur J Biochem 232:658-63. 1995..It was shown to be identical to an authentic, synthetic sample. From this, it is evident that E375 is the active-site nucleophile of 6-phospho-galactosidase, consistent with previous findings for enzymes in this family...
- Comparative biochemical and molecular analysis of the Staphylococcus hyicus, Staphylococcus aureus and a hybrid lipase. Indication for a C-terminal phospholipase domainK Nikoleit
Universitat Tubingen, Germany
Eur J Biochem 228:732-8. 1995..hyicus lipase. The fact that two closely related enzymes differ in the need for Ca2+ underscores the notion that it plays a structural rather than a catalytic role...
- A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterizationT Oshida
Rockefeller University, New York, NY 10021
Proc Natl Acad Sci U S A 92:285-9. 1995..aureus...
- Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interactJ I Ross
Department of Microbiology, University of Leeds, UK
Gene 153:93-8. 1995..There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact...
- Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureusM Sugai
Department of Microbiology, Hiroshima University School of Dentistry, Japan
J Bacteriol 179:2958-62. 1997....
- Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins familyA Gravet
UPRES EA 1318, Institut de Bacteriologie de la Faculte de Medecine, Université Louis Pasteur Hôpitaux Universitaires de Strasbourg, France
FEBS Lett 436:202-8. 1998..005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus...
- Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureusD Ni Eidhin
Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
Mol Microbiol 30:245-57. 1998....
- Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureusN Noguchi
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
FEMS Microbiol Lett 172:247-53. 1999..Our results indicated that four or more antiseptic-resistance genes exist in methicillin-resistant S. aureus and that antiseptic-resistant methicillin-resistant S. aureus strains without qacA and smr are widely spread in Japan...
- Antibacterial action of extracellular mammalian group IIA phospholipase A2 against grossly clumped Staphylococcus aureusM E Dominiecki
Department of Microbiology, New York University School of Medicine, New York, New York 10016, USA
Infect Immun 67:2299-305. 1999..Thus, the extracellular mobilization of group IIA PLA2 during inflammation may provide a mechanism by which the host can control the proliferation and survival of S. aureus even after bacterial clumping...
- Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesisA Bouhss
Biochimie Structurale et Cellulaire, CNRS, Batiment 430, Universite Paris Sud, F 91405, Orsay, France
Mol Microbiol 34:576-85. 1999..It was noteworthy that S. aureus MraY was functional in E. coli. An increased MraY transferase activity was observed only with the untruncated hybrids from both organisms...
- An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190K Kaneda
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
Proc Natl Acad Sci U S A 98:932-7. 2001..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
- Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureusM Bischoff
Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
J Bacteriol 183:5171-9. 2001..The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner...
- Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillinV K Singh
Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790, USA
Microbiology 147:3037-45. 2001..An insertional knockout mutation in the first gene of this operon resulted in increased susceptibility of the mutant to H2O2 compared to the parent strain, but not to oxacillin...
- A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor AChampion C S Deivanayagam
Center for Biophysical Sciences and Engineering, School of Optometry, 244 CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
EMBO J 21:6660-72. 2002....
- MprF-mediated lysinylation of phospholipids in Staphylococcus aureus leads to protection against oxygen-independent neutrophil killingSascha A Kristian
Microbial Genetics, University of Tubingen, 72076 Tubingen, Germany
Infect Immun 71:546-9. 2003..These results demonstrate the impact and specificity of MprF-mediated CAMP resistance and underscore the role of defensin-like peptides in innate host defense...
- Studies on the expression of regulatory locus sae in Staphylococcus aureusAna T Giraudo
Departamento de Microbiologia e Inmunologia, Facultad de Ciencias Exactas Físico, Química y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, Cordoba, Argentina
Curr Microbiol 46:246-50. 2003..This work, as well as previous studies, reveals a complex hierarchical regulatory network involving several loci that control the expression of virulence determinants in S. aureus...
- Crystal structure of the SarS protein from Staphylococcus aureusRonggui Li
Integrated Department of Immunology, National Jewish Medical and Research Center, and Department of Pharmacology, Biomolecular Structure Program, School of Medicine, University of Colorado Health Science Center, Denver, Colorado 80206, USA
J Bacteriol 185:4219-25. 2003..Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed...
- MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuationJutta Rossi
Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
Antimicrob Agents Chemother 47:2558-64. 2003..These observations suggest that MsrR is a new component involved in sarA attenuation and the regulatory network controlling virulence gene expression in S. aureus...
- Latent LytM at 1.3A resolutionSergey G Odintsov
International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
J Mol Biol 335:775-85. 2004..aureus supernatant. The "asparagine switch" in LytM is analogous to the "cysteine switch" in pro-matrix metalloproteases...
- Expression of staphylococcal clumping factor A impedes macrophage phagocytosisNiklas Palmqvist
Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10 A, S 413 46 Goteborg, Sweden
Microbes Infect 6:188-95. 2004..Both the protection against phagocytosis and the enhanced immunostimulatory activity provided by ClfA expression are likely to contribute to the in vivo virulence of S. aureus...
- Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehydeLuan Tao
Biological and Chemical Sciences and Engineering, Central Research and Development, E I DuPont de Nemours Inc, E328 B48, Wilmington, Deleware 19880 0328, USA
Appl Environ Microbiol 71:3294-301. 2005..coli as the predominant carotenoid. This C30 dialdehyde is a dark-reddish purple pigment that may have potential uses in foods and cosmetics...
- SarA positively controls bap-dependent biofilm formation in Staphylococcus aureusMaria Pilar Trotonda
Instituto Valenciano de Investigaciones Agrarias, Carretera Náquera Moncada Km 4, 5, 46113 Moncada, Valencia, Spain
J Bacteriol 187:5790-8. 2005..Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus...
- MepR, a repressor of the Staphylococcus aureus MATE family multidrug efflux pump MepA, is a substrate-responsive regulatory proteinGlenn W Kaatz
The John D Dingell Department of Veterans Affairs Medical Center, and Department of Medicine, Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI 48201, USA
Antimicrob Agents Chemother 50:1276-81. 2006....
- Regulation of Staphylococcus aureus alpha-toxin gene (hla) expression by agr, sarA, and sae in vitro and in experimental infective endocarditisYan Q Xiong
Department of Medicine, Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor University of California Los Angeles Medical Center, Torrance, CA, USA
J Infect Dis 194:1267-75. 2006..However, their relative in vivo contributions to hla expression, particularly in endovascular infections, have not been defined...
- Haem recognition by a Staphylococcus aureus NEAT domainJason C Grigg
Department of Microbiology and Immunology, Life Sciences Institute, The University of British Columbia, Vancouver, BC, Canada V6T 1Z3
Mol Microbiol 63:139-49. 2007..An analysis of IsdA structure-sequence alignments indicate that conservation of Tyr(166) is a predictor of haem binding by NEAT domains...
- Fibrinogen and elastin bind to the same region within the A domain of fibronectin binding protein A, an MSCRAMM of Staphylococcus aureusFiona M Keane
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
Mol Microbiol 63:711-23. 2007....
- Key role for clumping factor B in Staphylococcus aureus nasal colonization of humansHeiman F L Wertheim
Erasmus MC, University Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
PLoS Med 5:e17. 2008..aureus colonizes the nose are still unknown. The staphylococcal cell-wall protein clumping factor B (ClfB) promotes adhesion to squamous epithelial cells in vitro and might be a physiologically relevant colonization factor...
- THERAPEUTIC APPROACHES TO BIOFILM ANTIBIOTIC RESISTANCETimothy Opperman; Fiscal Year: 2005..We will develop these compounds into innovative drugs that will be used in combination with clinically relevant antibiotics to eradicate biofilm infections. ..
- c-diGMP Signal Transduction Proteins are Biofilm Drug TargetsTimothy Opperman; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Proline uptake in staphylococcus aureus pathogenesisWILLIAM SCHWAN; Fiscal Year: 2002..The results of this study will help us understand the role of proline transport in S. aureus infections. ..
- Gordon Research Conference on Staphylococcal DiseasesBRIGITTE BERGER BACHI; Fiscal Year: 2003..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..
- OmpR and acid regulation of Escherichia coli fim genesWILLIAM SCHWAN; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- Nucleotide Methylation in Ribosome MaturationJASON RIFE; Fiscal Year: 2008..Ultimately, these studies will reveal molecular details of methyltransferase reactions as well as likely provide the first evidence for a universal step in ribosome biogenesis. ..
- CF EPITHELIAL CELLS: INTERACTIONS WITH PSEUDOMONASAlice Prince; Fiscal Year: 2005..Using genetically defined mutants, the contribution of PLC to epithelial damage in normal and CF cells will be examined. ..
- Novel Therapies for BioFilm-Related InfectionsTimothy Opperman; Fiscal Year: 2005..epidermidis. The work in Phase I will provide all the molecular genetic tools and screening methods needed in Phase II for the discovery of potent small molecule inhibitors of staphylococcal biofilm formation. ..
- Binding and regulation mechanism for S aureusAmbrose Cheung; Fiscal Year: 2006..An understanding of these mechanisms will facilitate the development of novel agents that interfere with the function of the SarA family and the ensuing synthesis of cell wall adhesins and toxins. ..
- Rat, a regulator of autolysis in S. aureusAmbrose Cheung; Fiscal Year: 2008..Our long-term goal is to promote autolysis with an anti-Rat or pro-SarV strategy without developing an untoward impact on virulence. ..
- IN VIVO GENE EXPRESSION IN STAPHYLOCCUS AUREUSAmbrose Cheung; Fiscal Year: 2009..Upon the completion of these studies, we hope to understand how sae is activated in vivo and what target genes sae impacts during infection. ..
- Characterization of sar-agr Interactions in S. aureusAMBROSE LIN YAU CHEUNG; Fiscal Year: 2010..Our goal is to understand how SarA controls target gene and how other intracellular regulators affect SarA expression in S. aureus. ..
- The toxin-antitoxin system of Staphylococcus aureus.AMBROSE LIN YAU CHEUNG; Fiscal Year: 2010..As therapy options against multidrug resistant S. aureus are limited, our screening assays will provide the initial compounds against MazE for the development of novel antimicrobial therapy against S. aureus ..
- BACTERIAL INDUCTION OF CYTOKINE EXPRESSION IN THE LUNGAlice Prince; Fiscal Year: 2005....
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