Genomes and Genes
Publications2058 found, 100 shown here
- Casein kinase II mediates multiple phosphorylation of Saccharomyces cerevisiae eIF-2 alpha (encoded by SUI2), which is required for optimal eIF-2 function in S. cerevisiaeL Feng
Department of Biology, Indiana University, Bloomington 47405
Mol Cell Biol 14:5139-53. 1994..that the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha), encoded by the SUI2 gene in the yeast Saccharomyces cerevisiae, is phosphorylated at Ser-51 by the GCN2 kinase in response to general amino acid control...
- Role of endoplasmic reticulum-derived vesicles in the formation of Golgi elements in sec23 and sec18 Saccharomyces Cerevisiae mutantsM N Morin Ganet
, CEA Saclay, France
Anat Rec 251:256-64. 1998BACKGROUND: In the yeast Saccharomyces cerevisiae, the Golgi apparatus consists of individual networks of membranous tubules interspersed throughout the cytoplasm...
- The effect of Saccharomyces cerevisiae and Aspergillus oryzae on the digestion of the cell wall fraction of a mixed diet in defaunated and refaunated sheep rumenJ P Jouany
INRA, SRNH, Centre de Clermont Theix, Saint Genes Champanelle, France
Reprod Nutr Dev 38:401-16. 1998The objective of this study was to determine the effect of two probiotics, Saccharomyces cerevisiae (SC) and Aspergillus oryzae (AO), without their culture medium, on the digestion of plant cell wall components in sheep that had been ..
- How Escherichia coli and Saccharomyces cerevisiae build Fe/S proteinsFrederic Barras
Laboratoire de Chimie Bacterienne, UPR CNRS 9043 and LRC CNRS CEA 35v, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille, France
Adv Microb Physiol 50:41-101. 2005..In Saccharomyces cerevisiae, the ISC system is located in the mitochondria and its function is necessary for maturation of both ..
- Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1 alpha and 1 beta (gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombeM Miyazaki
Department of Molecular Biology, School of Science, Nagoya University, Aichi
J Biochem 103:508-21. 1988..1 alpha (EF-1 alpha) [corrected] was purified to homogeneity in high yield from the two different yeasts Saccharomyces carlsbergensis (S. carls.) and Schizosaccharomyces pombe (S. pombe)...
- Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporterT Kasahara
Laboratory of Biophysics, School of Medicine, Teikyo University, Tokyo, Japan
Biochim Biophys Acta 1324:111-9. 1997Rat Glut4 glucose transporter was expressed in the yeast Saccharomyces cerevisiae, but was retained in an intracellular membranous compartment and did not contribute to glucose uptake by intact cells...
- Carbon source-dependent regulation of cell growth by murine protein kinase C epsilon expression in Saccharomyces cerevisiaeA M Parissenti
Department of Research, Northeastern Ontario Regional Cancer Centre, Sudbury, Canada
J Cell Physiol 178:216-26. 1999..by mammalian PKC isoforms may be effectively studied in a simpler genetically-accessible model system such as Saccharomyces cerevisiae. With this objective in mind, we transfected S...
- Fatty acid-responsive control of mRNA stability. Unsaturated fatty acid-induced degradation of the Saccharomyces OLE1 transcriptC I Gonzalez
Department of Biological Sciences and the Bureau of Biological Research, Nelson Laboratories, Piscataway, New Jersey 08855 1059, USA
J Biol Chem 271:25801-9. 1996The Saccharomyces cerevisiae OLE1 gene encodes the Delta-9 fatty acid desaturase, a highly regulated integral membrane enzyme involved in the formation of unsaturated fatty acids from saturated acyl-coenzyme A precursors...
- Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activitiesR G Brooke
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208
J Biol Chem 266:3005-15. 1991The yeast Saccharomyces cerevisiae catalytic DNA polymerase I 180-kDa subunit and the tightly associated 86-kDa polypeptide have been purified using immunoaffinity chromatography, permitting further characterization of the DNA polymerase ..
- The complement of protein kinases of the microsporidium Encephalitozoon cuniculi in relation to those of Saccharomyces cerevisiae and Schizosaccharomyces pombeDiego Miranda-Saavedra
College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, Scotland, UK
BMC Genomics 8:309. 2007..cuniculi genomic database aimed at identifying and classifying all protein kinases of this organism with reference to the kinomes of two highly-divergent yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe.
- Potentiometric and further kinetic characterization of the flavin-binding domain of Saccharomyces cerevisiae flavocytochrome b2. Inhibition by anions binding in the active siteNarimantas Cenas
Institute of Biochemistry, Mokslininku 12, Vilnius 2600, Lithuania
Biochemistry 46:4661-70. 2007b>Saccharomyces cerevisiae flavocytochrome b2 (L-lactate:cytochrome c oxido reductase, EC 18.104.22.168) is a homotetramer, with FMN and protoheme IX binding on separate domains...
- Growth performance and gastrointestinal microbial ecology responses of piglets receiving Saccharomyces cerevisiae fermentation products after an oral challenge with Escherichia coli (K88)E Kiarie
Department of Animal Science, Winnipeg, Manitoba R3T 2N2, Canada
J Anim Sci 89:1062-78. 2011The effects of Saccharomyces cerevisiae fermentation products (YFP) on growth performance and gastrointestinal (GIT) microbial ecology in 90 weanling pigs orally challenged with Escherichia coli K88(+) (ETEC) were investigated...
- Localization and kinetics of pyruvate-metabolizing enzymes in relation to aerobic alcoholic fermentation in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621H van Urk
Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands
Biochim Biophys Acta 992:78-86. 1989The role of pyruvate metabolism in the triggering of aerobic, alcoholic fermentation in Saccharomyces cerevisiae has been studied. Since Candida utilis does not exhibit a Crabtree effect. this yeast was used as a reference organism...
- 3-Isopropylmalate is the major endogenous substrate of the Saccharomyces cerevisiae trans-aconitate methyltransferaseJonathan E Katz
Department of Chemistry and Biochemistry, The Molecular Biology Institute, Los Angeles, California 90095 1570, USA
Biochemistry 43:5976-86. 2004The Saccharomyces cerevisiae Tmt1 gene product is the yeast homologue of the Escherichia coli enzyme that catalyzes the methyl esterification of trans-aconitate, a thermodynamically favored isomer of cis-aconitate and an inhibitor of the ..
- Cloning and bacterial expression of the CYS3 gene encoding cystathionine gamma-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the proteinS Yamagata
Department of Biology, Faculty of General Education, Gifu University, Japan
J Bacteriol 175:4800-8. 1993..we isolated and characterized a gene rescuing the cysteine requirement in a "cys1" strain of Saccharomyces cerevisiae...
- Reevaluation of the 9 compounds reported conclusive positive in yeast Saccharomyces cerevisiae aneuploidy test systems by the Gene-Tox Program using strain D61.M of Saccharomyces cerevisiaeS Albertini
Department of Toxicology, F Hoffmann La Roche, Ltd, Basel, Switzerland
Mutat Res 260:165-80. 1991..S. Environmental Protection Agency in 1986 in analyzing published data. In Saccharomyces cerevisiae 9 chemicals were reported to be conclusive positive for aneuploidy induction in either mitotic or ..
- [Saccharomyces boulardii modulates dendritic cell properties and intestinal microbiota disruption after antibiotic treatment]A Collignon
EA 4043, USC INRA Ecosystème Microbien Digestif et Santé, Pharmacy Faculty, Paris Sud XI University, Chatenay Malabry Cedex, France
Gastroenterol Clin Biol 34:S71-8. 2010b>Saccharomyces boulardii is a non-pathogenic yeast with biotherapeutic properties that has been used successfully to prevent and to treat various infectious and antibiotic-associated diarrheas...
- [Saccharomyces cerevisiae B5 efficiently and stereoselectively reduces 2'-chloroacetophenone to R-2'-chloro-1-phenylethanol in the presence of 5% ethanol]Zhi min Ou
Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
Sheng Wu Gong Cheng Xue Bao 19:206-11. 2003..In this communication, we describe (1) the identification of Saccharomyces cerevisiae B5 as an effective host for stereoselective reduction of 2'-chloroacetophenone to (R)-2'-chloro-1-..
- Somatic recombination, gene amplification and cancerC Ramel
Department of Genetic and Cellular Toxicology, Stockholm University, Sweden
Mutat Res 353:85-107. 1996..These have included Drosophila, Saccharomyces and mammalian cell cultures. 6.1. Screening assays for mitotic recombination...
- Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the geneE Gonzalez
Department of Biochemistry and Molecular Biology, Faculty of Sciences, Universitat Autonoma de Barcelona, E 08193 Bellaterra Barcelona, and Spain
J Biol Chem 275:35876-85. 2000The completion of the Saccharomyces cerevisiae genome project in 1996 showed that almost 60% of the potential open reading frames of the genome had no experimentally determined function...
- Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 geneC V Heath
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
J Cell Biol 131:1677-97. 1995..nucleus to the cytoplasm, we used an in situ hybridization assay to screen temperature-sensitive strains of Saccharomyces cerevisiae...
- Successful design and development of genetically engineered Saccharomyces yeasts for effective cofermentation of glucose and xylose from cellulosic biomass to fuel ethanolN W Ho
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907 1295, USA
Adv Biochem Eng Biotechnol 65:163-92. 1999..cellulosic biomass contains two major sugars, glucose and xylose, and a major obstacle in this process is that Saccharomyces yeasts, traditionally used and still the only microorganisms currently used for large scale industrial ..
- Bioinformatics analysis of a Saccharomyces cerevisiae N-terminal proteome provides evidence of alternative translation initiation and post-translational N-terminal acetylationKenny Helsens
Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
J Proteome Res 10:3578-89. 2011..N-terminal COFRADIC (combined fractional diagonal chromatography), we here isolated N-terminal peptides of a Saccharomyces cerevisiae proteome and analyzed both annotated and alternative TIS. We analyzed this N-terminome of S...
- Crystal structure of Saccharomyces cerevisiae 6-phosphogluconate dehydrogenase Gnd1Weiwei He
Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, People s Republic of China
BMC Struct Biol 7:38. 2007..The 3D structural investigation would be very valuable in designing small molecules that target this enzyme for potential therapeutic applications...
- Isolation of constitutive mutations affecting the proline utilization pathway in Saccharomyces cerevisiae and molecular analysis of the PUT3 transcriptional activatorJ E Marczak
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark 07103
Mol Cell Biol 9:4696-705. 1989The enzymes of the proline utilization pathway (the products of the PUT1 and PUT2 genes) in Saccharomyces cerevisiae are coordinately regulated by proline and the PUT3 transcriptional activator...
- Overall kinetic mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiaeHengyu Xu
Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA
Biochemistry 45:12156-66. 2006Kinetic data have been measured for the histidine-tagged saccharopine dehydrogenase from Saccharomyces cerevisiae, suggesting the ordered addition of nicotinamide adenine dinucleotide (NAD) followed by saccharopine in the physiologic ..
- Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae: importance of xylulokinase (XKS1) and oxygen availabilityM H Toivari
VTT Biotechnology, P O Box 1500, FIN 02044 VTT, Finland
Metab Eng 3:236-49. 2001The yeast Saccharomyces cerevisiae efficiently ferments hexose sugars to ethanol, but it is unable to utilize xylose, a pentose sugar abundant in lignocellulosic materials...
- Further phenotypic characterization of pso mutants of Saccharomyces cerevisiae with respect to DNA repair and response to oxidative stressCristina Pungartnik
Departamento de Biofisica Centro de Biotecnologia, UFRGS, Av Bento Goncalves, 9500, 91507 970 Porto Alegre, RS, Brazil
Genet Mol Res 1:79-89. 2002The sensitivity responses of seven pso mutants of Saccharomyces cerevisiae towards the mutagens N-nitrosodiethylamine (NDEA), 1,2:7,8-diepoxyoctane (DEO), and 8-hydroxyquinoline (8HQ) further substantiated their allocation into two ..
- Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadioneIl Sup Kim
Department of Microbiology, Kyungpook National University, Daegu, 702 701, Republic of Korea
Cell Stress Chaperones 16:1-14. 2011..The accumulation of Cpr1 protein to menadione in Saccharomyces cerevisiae KNU5377Y suggests a possibility that this protein may participate in the mechanism of stress ..
- Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domainY Zheng
Department of Pharmacology, Cornell University, Ithaca, New York 14853
J Biol Chem 268:24629-34. 1993The Bem2 and Bem3 proteins, which appear to play roles in the regulation of bud site formation in Saccharomyces cerevisiae, show striking homology to a number of proteins that compose a family of GTPase-activating proteins (GAPs) for the ..
- Anti-Saccharomyces cerevisiae mannan antibodies in inflammatory bowel disease: comparison of different assays and correlation with clinical featuresV Annese
Divisione di Gastroenterologia, Ospedale CSS IRCCS, San Giovanni Rotondo, Italy
Aliment Pharmacol Ther 20:1143-52. 2004Anti-Saccharomyces cerevisiae mannan antibodies have been proposed as a new serological marker associated with Crohn's disease. However, their clinical value is still unclear; furthermore, a standardization of anti-S...
- Isolation and characterization of COX12, the nuclear gene for a previously unrecognized subunit of Saccharomyces cerevisiae cytochrome c oxidaseA E LaMarche
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
J Biol Chem 267:22473-80. 1992We have cloned and sequenced COX12, the nuclear gene for subunit VIb of Saccharomyces cerevisiae cytochrome c oxidase. This subunit, which was previously not found in cytochrome c oxidase purified from S...
- Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acidsA G Mitchell
Department of Biological Sciences and the Bureau of Biological Research, Rutgers University, Nelson Laboratories, P O Box 1059, Piscataway, New Jersey 08855 1059, USA
J Biol Chem 272:28281-8. 1997A search of the Saccharomyces cerevisiae genome data base for cytochrome b5-like sequences identified a 1.152-kilobase pair open reading frame, located on chromosome XIII at locus YMR272C (FAH1)...
- The effects of Saccharomyces cerevisiae extract on the weight of some organs, liver, and pancreatic digestive enzyme activity in breeder hens fed diets contaminated with aflatoxinsE Matur
Department of Physiology, Faculty of Veterinary Medicine, University of Istanbul Avcilar, and Dr Sadi Konuk Research and Training Hospital, Istanbul, 34320, Turkey
Poult Sci 89:2213-20. 2010The effects of the Saccharomyces cerevisiae extract on some organ, liver, and pancreatic digestive enzymes in breeder hens fed on aflatoxin (AF)-contaminated feed were investigated. Forty-eight 58-wk-old Ross 308 breeder hens were used...
- Physiological effects of nitrogen starvation in an anaerobic batch culture of Saccharomyces cerevisiaeU Schulze
Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark
Microbiology 142:2299-310. 1996The effects of nitrogen starvation on the anaerobic physiology of Saccharomyces cerevisiae were studied in cells cultivated in a bioreactor...
- Metabolism of sulfur amino acids in Saccharomyces cerevisiaeD Thomas
Centre de Genetique Moleculaire, CNRS, Gif sur Yvette, France
Microbiol Mol Biol Rev 61:503-32. 1997Sulfur amino acid biosynthesis in Saccharomyces cerevisiae involves a large number of enzymes required for the de novo biosynthesis of methionine and cysteine and the recycling of organic sulfur metabolites...
- Dietary administration of the probiotic, Saccharomyces cerevisiae P13, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioidesChiu Hsia Chiu
Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan, ROC
Fish Shellfish Immunol 29:1053-9. 2010..The survival of Saccharomyces cerevisiae P13 in the posterior intestines using a specific primer pair of YMR245w-F/YMR245w-R, non-specific ..
- Roles of URE2 and GLN3 in the proline utilization pathway in Saccharomyces cerevisiaeS Xu
Department of Microbiology and Molecular Genetics, UMD New Jersey Medical School, Newark 07103
Mol Cell Biol 15:2321-30. 1995The yeast Saccharomyces cerevisiae can use alternative nitrogen sources such as arginine, urea, allantoin, gamma-aminobutyrate, or proline when preferred nitrogen sources like glutamine, asparagine, or ammonium ions are unavailable in ..
- Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylationD R Johnson
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110
J Biol Chem 269:18037-46. 1994NMT1 is an essential Saccharomyces cerevisiae gene which encodes myristoyl-CoA:protein N-myristoyltransferase (Nmt1p)...
- Flavour formation in fungi: characterisation of KlAtf, the Kluyveromyces lactis orthologue of the Saccharomyces cerevisiae alcohol acetyltransferases Atf1 and Atf2Stijn D M Van Laere
Centre for Malting and Brewing Science, Centre for Food and Microbial Technology, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, 3001 Leuven Heverlee, Belgium
Appl Microbiol Biotechnol 78:783-92. 2008..In the brewers' yeast Saccharomyces cerevisiae, the major part of these esters is formed by two alcohol acetyltransferases, Atf1 and Atf2...
- Peroxisomal degradation of trans-unsaturated fatty acids in the yeast Saccharomyces cerevisiaeA Gurvitz
Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
J Biol Chem 276:895-903. 2001Degradation of trans-unsaturated fatty acids was studied in the yeast Saccharomyces cerevisiae...
- Fermentative behavior of Saccharomyces strains during microvinification of raspberry juice (Rubus idaeus L.)Whasley F Duarte
Department of Biology, Federal University of Lavras UFLA, CP 3037, Campus Universitario, CEP 37 200 000 Lavras, MG, Brazil
Int J Food Microbiol 143:173-82. 2010Sixteen different strains of Saccharomyces cerevisiae and Saccharomyces bayanus were evaluated in the production of raspberry fruit wine...
- The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like proteinJ Wittmeyer
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
Mol Cell Biol 17:4178-90. 1997..alpha affinity chromatography to identify factors involved in eukaryotic DNA replication in the yeast Saccharomyces cerevisiae...
- The protective effects of a fermented substance from Saccharomyces cerevisiae on carbon tetrachloride-induced liver damage in ratsJinn Tsyy Lai
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC
Clin Nutr 28:338-45. 2009The aim of this study was to investigate the effect of a fermented substance from Saccharomyces cerevisiae (FSSC) on the liver fibrosis induced by chronic carbon tetrachloride (CCl(4)) administration in rats.
- Repair of oxidative damage in mitochondrial DNA of Saccharomyces cerevisiae: involvement of the MSH1-dependent pathwayPiotr Dzierzbicki
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
DNA Repair (Amst) 3:403-11. 2004..A series of genetic and biochemical studies has indicated that eukaryotic cells, including the model organism Saccharomyces cerevisiae, use several alternative strategies to prevent mutagenesis induced by endogenous oxidative damage ..
- Purification, characterization and biological evaluation of recombinant leech-derived tryptase inhibitor (rLDTI) expressed at high level in the yeast Saccharomyces cerevisiaeG Pohlig
Department of Core Drug Discovery Technologies, Ciba Geigy Ltd, Basel, Switzerland
Eur J Biochem 241:619-26. 1996..LDTI was expressed in the yeast Saccharomyces cerevisiae under the control of the copper-inducible CUP1 promoter and fused to the invertase signal sequence (..
- Global mRNA expression analysis in myosin II deficient strains of Saccharomyces cerevisiae reveals an impairment of cell integrity functionsJosé F Rodríguez-Quiñones
Department of Biochemistry, School of Medicine, Medical Sciences Campus, University of Puerto Rico, P O Box 365067, San Juan, Puerto Rico 00936 5067, USA
BMC Genomics 9:34. 2008The Saccharomyces cerevisiae MYO1 gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast...
- NAT2, an essential gene encoding methionine N alpha-acetyltransferase in the yeast Saccharomyces cerevisiaeM S Kulkarni
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642
J Biol Chem 269:13141-7. 1994..NAT1 and ARD1 from the yeast Saccharomyces cerevisiae (Mullen, J. R., Kayne, P. S., Moerschell, R. P., Tsunasawa, S., Gribskov, M., Colavito-Shepanski, M...
- Expression of high-affinity glucose transport protein Hxt2p of Saccharomyces cerevisiae is both repressed and induced by glucose and appears to be regulated posttranslationallyD L Wendell
Department of Viticulture and Enology, University of California, Davis 95616 8749
J Bacteriol 176:3730-7. 1994Expression of putative high-affinity glucose transport protein Hxt2p of Saccharomyces cerevisiae was repressed 15- to 20-fold in high concentrations of glucose or fructose. S...
- Phylogenetic analysis of the Saccharomyces cerevisiae group based on polymorphisms of rDNA spacer sequencesR Montrocher
CNRS, UMR 5557, Ecologie Microbienne du Sol, Universite Lyon 1, Villeurbanne, France
Int J Syst Bacteriol 48:295-303. 1998The phylogenetic relationships between species of yeasts assigned to the Saccharomyces sensu stricto group, which includes Saccharomyces cerevisiae and Saccharomyces bayanus, were studied together with Saccharomyces pastorianus and ..
- A genome-wide deletion mutant screen identifies pathways affected by nickel sulfate in Saccharomyces cerevisiaeAdriana Arita
New York University School of Medicine, Nelson Institute of Environmental Medicine, NY 10987, USA
BMC Genomics 10:524. 2009..the high conservation in gene function found between yeast genes and their human homologues, has allowed for Saccharomyces cerevisiae to be used as a model organism to deduce biological processes in human cells...
- Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesisC Mo
Indiana University Purdue University Indianapolis, Biology Department, 723 West Michigan Street, Indianapolis, IN 46202, USA
Proc Natl Acad Sci U S A 99:9739-44. 2002A Saccharomyces cerevisae microarray expression study indicated that an ORF, YER044C, now designated ERG28, was strongly coregulated with ergosterol biosynthesis...
- Regulation of heat shock factor in Schizosaccharomyces pombe more closely resembles regulation in mammals than in Saccharomyces cerevisiaeG J Gallo
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
Mol Cell Biol 11:281-8. 1991..This regulation is in contrast to that observed in Saccharomyces cerevisiae, in which HSF binding is detectable at both normal and heat shock temperatures. The S...
- UniPROBE: an online database of protein binding microarray data on protein-DNA interactionsDaniel E Newburger
Division of Genetics, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School and Harvard MIT Division of Health Sciences and Technology, Harvard Medical School, Boston, MA 02115, USA
Nucleic Acids Res 37:D77-82. 2009..malarial parasite Plasmodium falciparum, the parasitic Apicomplexan Cryptosporidium parvum, the yeast Saccharomyces cerevisiae, the worm Caenorhabditis elegans, mouse and human...
- A set of epitope-tagging integration vectors for functional analysis in Saccharomyces cerevisiaeHyeran Sung
College of Pharmacy, Chungbuk National University, 48 Gaeshindong, Cheongju, Chungbuk, Republic of Korea
FEMS Yeast Res 5:943-50. 2005Functional analysis of genes from Saccharomyces cerevisiae has been the major goal after determination of genome sequences...
- Peroxisomal localisation of the final steps of the mevalonic acid pathway in plantaAndrew J Simkin
EA 2106, Biomolecules et Biotechnologies Vegetales, Universite Francois Rabelais de Tours, 31 Avenue Monge, 37200, Tours, France
Planta 234:903-14. 2011..These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C...
- Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteinsL Zheng
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center San Antonio, San Antonio, Texas 78245, USA
Mol Cell Biol 19:5417-28. 1999..A Saccharomyces cerevisiae protein, identical to Tid3p/Ndc80p and here designated scHec1p, has similarities in structure and ..
- Pleofungins, novel inositol phosphorylceramide synthase inhibitors, from Phoma sp. SANK 13899. I. Taxonomy, fermentation, isolation, and biological activitiesTatsuya Yano
Lead Discovery Research Laboratories, Sankyo Co, Ltd, Hiromachi, Tokyo, Japan
J Antibiot (Tokyo) 60:136-42. 2007..Pleofungin A inhibited the IPC synthase of Saccharomyces cerevisiae and Aspergillus fumigatus at IC(50) values of 16 and 1.0 ng/ml, respectively...
- Isolation and functional analysis of yeast ubiquitin ligase Rsp5 variants that alleviate the toxicity of human α-synucleinIndah Wijayanti
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0192, Japan
J Biochem 157:251-60. 2015..ligase Rsp5 is a key enzyme involved in the degradation of abnormal or unfavourable proteins in the yeast Saccharomyces cerevisiae...
- The gene encoding the phosphatidylinositol transfer protein is essential for cell growthJ F Aitken
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225
J Biol Chem 265:4711-7. 1990..An oligonucleotide based on the amino-terminal sequence of the PI-TP from Saccharomyces cerevisiae was used to screen a yeast genomic library for the gene encoding PI-TP (PIT1 gene)...
- Yeast Barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiersZhun Yan
Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Nat Methods 5:719-25. 2008..For example, highly parallel chemical-genetic screens using pooled collections of thousands of defined Saccharomyces cerevisiae gene deletion strains are feasible because each strain is bar-coded with unique DNA sequences...
- Quantifying variation in the ability of yeasts to attract Drosophila melanogasterLoida Palanca
School of Biological Sciences, The University of Auckland, Auckland, New Zealand
PLoS ONE 8:e75332. 2013..Attractiveness varied significantly by yeast, with the strongly fermenting Saccharomyces species generally being more attractive than the mostly respiring non-Saccharomyces species (P = 0.0035)...
- The ZIP family zinc transporters support the virulence of Cryptococcus neoformansEunsoo Do
Department of Systems Biotechnology, Chung Ang University, Anseong, 456 756, Republic of Korea
Med Mycol 54:605-15. 2016..pathogenic fungus Cryptococcus neoformans Zip1 and Zip2 are homologous to Zrt1 and Zrt2 of the model fungus, Saccharomyces cerevisiae, respectively...
- Slk19-dependent mid-anaphase pause in kinesin-5-mutated cellsNatalia Movshovich
Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva, Israel
J Cell Sci 121:2529-39. 2008We examined spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor proteins Cin8 and Kip1...
- Chromosome-wide histone deacetylation by sirtuins prevents hyperactivation of DNA damage-induced signaling upon replicative stressAntoine Simoneau
Maisonneuve Rosemont Hospital Research Center, 5415 Assomption Boulevard, Montreal, H1T 2M4, Canada Molecular Biology Program, Universite de Montreal, P O Box 6128, Succursale Centre Ville, Montreal, H3C 3J7, Canada
Nucleic Acids Res 44:2706-26. 2016The Saccharomyces cerevisiae genome encodes five sirtuins (Sir2 and Hst1-4), which constitute a conserved family of NAD-dependent histone deacetylases. Cells lacking any individual sirtuin display mild growth and gene silencing defects...
- Distribution and maintenance of histone H3 lysine 36 trimethylation in transcribed locusHenel Sein
Department of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu, 51010, Estonia
PLoS ONE 10:e0120200. 2015..In Saccharomyces cerevisiae methylation marks at K4, K36, and K79 of histone H3 are associated with gene transcription...
- Caloric restriction or catalase inactivation extends yeast chronological lifespan by inducing H2O2 and superoxide dismutase activityAna Mesquita
Instituto de Investigação em Ciências da Vida e Saúde, Escola de Ciências da Saúde, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Proc Natl Acad Sci U S A 107:15123-8. 2010..Here we show that, in Saccharomyces cerevisiae, caloric restriction or inactivation of catalases extends chronological lifespan by inducing ..
- Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viabilityM E Schmitt
Department of Developmental Biology, Stanford University School of Medicine, California 94305 5427
Genes Dev 6:1975-85. 1992..The same activity in the yeast Saccharomyces cerevisiae has recently been identified; it cleaves an RNA substrate complementary to a yeast mitochondrial ..
- Near infrared spectroscopy as high-throughput technology for screening of xylose-fermenting recombinant Saccharomyces cerevisiae strainsHiroyuki Morita
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
Anal Chem 83:4023-9. 2011Recently, genetic engineering efforts have been made to develop recombinant Saccharomyces cerevisiae strains able to utilize xylose, an inexpensive and abundant carbon source...
- The effect of phytase enzyme and level on nutrient extraction by broilersF G Silversides
Crops and Livestock Research Centre, Charlottetown, Prince Edward Island, Canada
Poult Sci 83:985-9. 2004Three experimental phytase enzyme preparations derived from the same Escherichia coli gene but produced in Saccharomyces cerevisiae (A), Pichia pastoris (B), and Pseudomonas fluorescens (C) were compared with a commercial enzyme ..
- Saccharomyces cerevisiae BUR6 encodes a DRAP1/NC2alpha homolog that has both positive and negative roles in transcription in vivoG Prelich
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Mol Cell Biol 17:2057-65. 1997..for mutations that increase transcription from an upstream activating sequence (UAS)-less promoter in Saccharomyces cerevisiae...
- Overexpression of Ycg1 or Ydr520c confers resistance to cadmium in Saccharomyces cerevisiaeGi Wook Hwang
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi 980 8578, Japan
J Toxicol Sci 34:441-3. 2009We introduced a yeast open reading frame library into Saccharomyces cerevisiae strain BY4742 to search for genes whose overexpression conferred cadmium resistance to yeast, toward the goal of elucidating the mechanism of cadmium toxicity...
- A novel subgradient-based optimization algorithm for blockmodel functional module identificationYijie Wang
Department of Computer Science and Engineering, University of South Florida, Tampa, FL 33620, USA
BMC Bioinformatics 14:S23. 2013..We have implemented our algorithm for large-scale protein-protein interaction (PPI) networks, including Saccharomyces cerevisia and Homo sapien PPI networks collected from the Database of Interaction Proteins (DIP) and Human ..
- Amino acid transport through the Saccharomyces cerevisiae Gap1 permease is controlled by the Ras/cAMP pathwayJinnie M Garrett
Department of Biology, Hamilton College, 198 College Hill Road, Clinton, NY 13323, USA
Int J Biochem Cell Biol 40:496-502. 2008The general amino acid permease (Gap1p) of Saccharomyces cerevisiae is a broad range, low affinity permease that imports amino acids in cells growing on poor nitrogen sources...
- Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome systemMin Kyung Sung
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125
Mol Biol Cell 27:2642-52. 2016..Ribosomal proteins cannot be overproduced in Saccharomyces cerevisiae because the excess proteins are rapidly degraded...
- DNA bending by Saccharomyces cerevisiae ABF1 and its proteolytic fragmentsL D McBroom
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada
J Biol Chem 269:16461-8. 1994The ABF1 protein of the yeast Saccharomyces cerevisiae has been found to bend the DNA containing the target site for DNA binding...
- The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complexA Platt
School of Biological Sciences, The University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
EMBO J 17:4086-91. 1998b>Saccharomyces cerevisiae responds to galactose as the sole source of carbon by activating the GAL genes encoding the enzymes of the Leloir pathway...
- Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiaeBrian C Smith
Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, United States
Biochemistry 51:243-56. 2012..We have performed structural and kinetic investigations of the nicotinamidase from Saccharomyces cerevisiae (Pnc1)...
- De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysisPolly M Fordyce
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
Nat Biotechnol 28:970-5. 2010..We validated our technique by measuring sequence preferences for 28 Saccharomyces cerevisiae transcription factors with a variety of DNA-binding domains, including several that have proven ..
- Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell linesNguyen Ngoc Hoa
Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto, 606 8501, Japan
Genes Cells 20:1059-76. 2015..DSB resection is initiated by CtIP and Mre11 followed by long-range resection by Dna2 and Exo1 in Saccharomyces cerevisiae...
- Inhibition of saturated very-long-chain fatty acid biosynthesis by mefluidide and perfluidone, selective inhibitors of 3-ketoacyl-CoA synthasesStefan Tresch
BASF SE, Crop Protection, Limburgerhof, Germany
Phytochemistry 76:162-71. 2012..To study compound effects on enzyme level, recombinant KCSs from Arabidopsis thaliana were expressed in Saccharomyces cerevisiae...
- Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiaeDong Hyuk Heo
School of Life Sciences and Biotechnology, Korea University Anam Dong, Sungbuk Gu, Seoul, Republic of Korea
Biochem J 431:257-65. 2010..We employed Saccharomyces cerevisiae as a model system to study cadmium toxicity at the molecular level because it has been used to ..
- Only one splice variant of the human TAZ gene encodes a functional protein with a role in cardiolipin metabolismFrederic M Vaz
Departments of Clinical Chemistry and Pediatrics, Emma Children s Hospital, Academic Medical Center, University of Amsterdam, P O Box 22700, 1100 DE Amsterdam, The Netherlands
J Biol Chem 278:43089-94. 2003..Like BTHS patients, a Saccharomyces cerevisiae strain, in which the yeast orthologue of the human TAZ gene has been disrupted, exhibits an ..
- Molecular cloning and expression of the hyu genes from Microbacterium liquefaciens AJ 3912, responsible for the conversion of 5-substituted hydantoins to alpha-amino acids, in Escherichia coliShun ichi Suzuki
AminoScience Laboratories, Ajinomoto Co, Inc, Kanagawa, Japan
Biosci Biotechnol Biochem 69:1473-82. 2005..The deduced amino acid sequences of hyuP were similar to those of the allantoin (5-ureido-hydantoin) permease from Saccharomyces cerevisiae, suggesting that hyuP protein might function as a hydantoin transporter.
- Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene familyL Rodriguez
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba
Yeast 14:1399-406. 1998..An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.
- Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation methodNatsuko Miura
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
Appl Microbiol Biotechnol 82:293-301. 2009..After cocultivation, EGFP fusion proteins produced by GM yeast were targeted to non-GM yeast (Saccharomyces cerevisiae BY4741DeltaCYC8 strain) cell surface...
- Analysis of Phylogenetic Relationships Among Twelve Yeast SpeciesPing Wu Zhang
Institute of Genetics, State Key Laboratory of Genetics, Fudan University, Shanghai 200433, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 31:477-478. 1999..We compared them with two published 25 S rDNA sequences of Saccharomyces cerevisiae and Candida albicans...
- Quantitative protein localization signatures reveal an association between spatial and functional divergences of proteinsLit Hsin Loo
Bioinformatics Institute, Agency for Science, Technology and Research, Singapore, Singapore Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
PLoS Comput Biol 10:e1003504. 2014..Using the budding yeast Saccharomyces cerevisiae as a model system, we show that PLAST is more accurate than existing, qualitative protein ..
- Conserved Sequence Preferences Contribute to Substrate Recognition by the ProteasomeHouqing Yu
From the Department of Molecular Biosciences and the Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208
J Biol Chem 291:14526-39. 2016..directly to the affinity of the initiation sites to their receptor on the proteasome and are conserved between Saccharomyces cerevisiae, Schizosaccharomyces pombe, and human cells...
- Constitutive expression of the DUR1,2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentationDianhui Wu
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, P R China National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, P R China School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, P R China
FEMS Microbiol Lett 363:fnv214. 2016..During fermentation, urea is generated from arginine by arginase in Saccharomyces cerevisiae, and subsequently cleaved by urea amidolyase or directly transported out of the cell into the ..
- Non-conventional yeasts as hosts for heterologous protein productionA Dominguez
Departamento de Microbiología y Genética Instituto de Microbiología Bioquímica CSIC, Universidad de Salamanca, Spain
Int Microbiol 1:131-42. 1998..b>Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces ..
- Repair of 8-oxo-7,8-dihydroguanine in prokaryotic and eukaryotic cells: Properties and biological roles of the Fpg and OGG1 DNA N-glycosylasesSerge Boiteux
Centre de Biophysique Moleculaire, CNRS, UPR4301, rue Charles Sadron, 45072 Orléans, France Electronic address
Free Radic Biol Med . 2016..In Saccharomyces cerevisiae, OGG1 cooperates with nucleotide excision repair (NER), mismatch repair (MMR), post-replication ..
- Organization of human replicon: singles or zipping couples?Anna Ligasová
Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, Academy of Sciences of the Czech Republic, Videnska 1083, 14200 Prague 4, Czech Republic
J Struct Biol 165:204-13. 2009..In prokaryotes and in budding yeast Saccharomyces cerevisiae, these two replisomes seem to be associated with one another until DNA replication initiated from ..
- Characterization of the Net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeastE E Traverso
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 276:21924-31. 2001In the budding yeast Saccharomyces cerevisiae, the multifunctional protein Net1 is implicated in regulating the cell cycle function of the Cdc14 protein phosphatase...
- Nutrient regulation of oligopeptide transport in Saccharomyces cerevisiaeAmy M Wiles
Department of Biochemistry, Cell and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA
Microbiology 152:3133-45. 2006Small peptides (2-5 amino acid residues) are transported into Saccharomyces cerevisiae via two transport systems: PTR (Peptide TRansport) for di-/tripeptides and OPT (OligoPeptide Transport) for oligopeptides of 4-5 amino acids in length...
- The fission yeast spo14+ gene encoding a functional homologue of budding yeast Sec12 is required for the development of forespore membranesMichiko Nakamura-Kubo
Department of Biology, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi ku, Japan
Mol Biol Cell 14:1109-24. 2003..spo14(+) is identical to the previously characterized stl1(+) gene encoding a putative homologue of Saccharomyces cerevisiae Sec12, which is essential for protein transport from the endoplasmic reticulum (ER) to the Golgi ..
- pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1P G Seeboth
Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Federal Republic of Germany
J Bacteriol 172:678-85. 1990The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 22.214.171.124) was deleted from the Saccharomyces cerevisiae genome...
- [A novel approach to isolation and functional characterization of genomic DNA from the methylotrophic yeast Hansenula polymorpha]M O Agafonov
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, 121552 Russia
Mol Biol (Mosk) 37:81-7. 2003..Analysis of the sequence encoding a homolog of the Saccharomyces cerevisiae cofilin revealed two introns. Another isolated DNA fragment encoded a homolog of the S...
- Human exonuclease 5 is a novel sliding exonuclease required for genome stabilityJustin L Sparks
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 287:42773-83. 2012Previously, we characterized Saccharomyces cerevisiae exonuclease 5 (EXO5), which is required for mitochondrial genome maintenance. Here, we identify the human homolog (C1orf176; EXO5) that functions in the repair of nuclear DNA damage...
- The J-protein family: modulating protein assembly, disassembly and translocationPeter Walsh
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Melbourne 3010, Australia
EMBO Rep 5:567-71. 2004..Sequence analysis on the genome of Saccharomyces cerevisiae has revealed 22 proteins that establish four distinguishing structural features of the J domain: ..
- The wheat MAP kinase phosphatase 1 confers higher lithium tolerance in yeastIkram Zaïdi
Laboratory of Plant Protection and Improvement, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia
FEMS Yeast Res 12:774-84. 2012The durum wheat TMKP1 gene encodes a MAP kinase phosphatase. When overexpressed in Saccharomyces cerevisiae, TMKP1 leads to salt stress tolerance (especially LiCl ), which is dependent on the phosphatase activity of the protein...
Research Grants121 found, 100 shown here
- James E Haber; Fiscal Year: 2016..the study of DSB repair by the most common pathway - gene conversion - in the model system, the budding yeast Saccharomyces cerevisiae...
- Mark L Siegal; Fiscal Year: 2014..In this project, a collection of 149 diploid MA lines of the genetically well-characterized yeast species, Saccharomyces cerevisiae, will be used...
- JEFFREY SCOTT SMITH; Fiscal Year: 2014..Sir2 from the budding yeast, Saccharomyces cerevisiae, is the founding family member and has been extensively studied for its role in transcriptional ..
- Facilitating large scale biomass generation: acquisition of an automated 100 L feRoger D Kornberg; Fiscal Year: 2010..investigate the structure and regulation of the RNA polymerase II transcription machinery from the yeast Saccharomyces cerevisiae...
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTElton T Young; Fiscal Year: 2013..In the simple single celled microbe, the budding yeast Saccharomyces cerevisiae, the simplest experimental paradigm for a nutrient shift is the response to loss of glucose, the ..
- Discovery of genes that extend yeast lifespan: Aging in immobilized cell reactorsRAPHAEL ROSENZWEIG; Fiscal Year: 2009..Many of our deepest insights into the biology of aging have been gained using the simple Eukaryote Saccharomyces cerevisiae...
- FASEB SRC on Yeast Chromosome Structure, Replication and SegregationScott Keeney; Fiscal Year: 2012..join investigators studying many diverse aspects of chromosome biology and cell biology in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe, using a range of experimental approaches including ..
- David P Bartel; Fiscal Year: 2016..Although RNAi has been lost in Saccharomyces cerevisiae, it is present in other budding yeasts, including Saccharomyces castellii (a close relative of S...
- Investigation of the structure and function of urea amidolyaseMARTIN RAOUL ST. MAURICE; Fiscal Year: 2012..Both Saccharomyces cerevisiae and C. albicans require UAL for urea-dependent growth...
- Crosstalk between the glucose signaling pathways in yeastJeong Ho Kim; Fiscal Year: 2013..The budding yeast Saccharomyces cerevisiae has a distinctive fermentative life style?the preferential conversion of glucose into ethanol even ..
- Identifying Conserved Genetic Networks for Eukaryotic MMR GenesWinfried Edelmann; Fiscal Year: 2012..to MMR in two highly divergent model eukaryotes, the fission (Schizosaccharomyces pombe;Sp) and budding (Saccharomyces cerevisiae;Sc) yeasts...
- A Study of DNA Replication Origins by Comparative Functional GenomicsIvan Liachko; Fiscal Year: 2012..This process has been most well studied in the budding yeast Saccharomyces cerevisiae, where origins can be studied as autonomously replicating sequences (ARSs) on plasmids...
- FACTORS CONTROLLING TRANSCRIPTION AND CHROMATIN IN YEASTFRED M WINSTON; Fiscal Year: 2013..application proposes to study fundamental aspects of transcription and chromatin regulation, using the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe...
- THE TRANSITIONAL ER-GOLGI SYSTEM IN BUDDING YEASTSBenjamin S Glick; Fiscal Year: 2012..These ideas will be explored with the aid of the budding yeasts Pichia pastoris and Saccharomyces cerevisiae. P. pastoris contains large, stable tER sites that are adjacent to Golgi stacks, whereas S...
- YEAST GENETIC RESEARCH RESOURCE CENTERJianlong Zhou; Fiscal Year: 2004..present, the yeast genetic collection at the ATCC contains 1,276 Yeast Genetic Stock Center (YGSC) strains of Saccharomyces (S.) cerevisiae, approximately 20,000 Saccharomyces Genome Deletion Project (SGDP) strains, over 1,400 ATCC S...
- Biochemical Characterization of Cytochrome b5 Reductase in Candida albicansMARY JOLENE PATRICIA HOLLOWAY; Fiscal Year: 2010..viability in response to pH, heat shock, and oxidative stress in Candida albicans and the yeast model system, Saccharomyces cerevisiae...
- Diverse Roles for RIC8 During G Protein Signaling in FungiKatherine A Borkovich; Fiscal Year: 2012..RIC8 is absent from plants, protists and the yeast Saccharomyces cerevisiae...
- Ai Ling Lin; Fiscal Year: 2016..CR perhaps is the most well-studied one for various model organisms of extended longevity, including Saccharomyces cerevisiae, Caenorhabditis elegans, rodents and monkeys...
- Evaluating the role of histone H3K79 methylation states in DNA damage repair in SJeffrey S Thompson; Fiscal Year: 2010..Using the yeast Saccharomyces cerevisiae as a model system, we have shown that loss of this modification renders cells hypersensitive to UV ..
- Mechanisms of Splicing Regulation During Environmental Stress in YeastJaclyn C Greimann; Fiscal Year: 2012DESCRIPTION (provided by applicant): Less than 5% of Saccharomyces cerevisiae genes have introns and most yeast intron-containing genes have only one intron...
- Mechanism of Translation Elongation Factor 2 Inhibition by Bacterial ToxinsTerri Goss Kinzy; Fiscal Year: 2010..Recent structural analysis of eEF2 from the yeast Saccharomyces cerevisiae, which is also modified by these toxins, demonstrates the diphthamide residue lies on the tip of ..
- Using Mass Spectroscopy to Characterize Transcription Proteins and F2-IsoprostaneBETTYE HENNINTON; Fiscal Year: 2011Project 1. Characterizing Phosphorylation Sites of Saccharomyces cerevisiae Transcription Factors, Rap1 and GcM Using Nanospray Mass Spectrometry Two Saccharomyces cerevisiae transcription factors, Raplp and Gcrlp, have been partially ..
- George M Carman; Fiscal Year: 2016DESCRIPTION (provided by applicant): In the yeast Saccharomyces cerevisiae, the PAH1 gene encodes phosphatidate phosphatase (Pah1p PAP), which has emerged as one of the most important and highly regulated enzymes in lipid metabolism...
- RNA polymerase II Elongation Complex: Structure and FunctionDaniel Reines; Fiscal Year: 2013..This has been best examined in the yeast Saccharomyces cerevisiae...
- Triazole hypersensitivity in Candida albicansNeeraj Chauhan; Fiscal Year: 2009..includes three HKs and two RRs, of which Ssk1p is a response regulator that is not functionally related to the Saccharomyces cerevisiae homologue...
- Activating liver carcinogens in yeast by expressing CYP450 polymorphismsMICHAEL THOMAS FASULLO; Fiscal Year: 2010..per se are sufficient to increase the genotoxicity of carcinogens, we have expressed the P450 genes in Saccharomyces cerevisiae (yeast), which lacks the detoxification enzymes...
- Montaser Shaheen; Fiscal Year: 2014..There is data to indicate a role of Pso4 in DNA repair from yeast to humans. Saccharomyces cerevisiae Mutant pso4...
- Centromere Structure and Function in Candida albicansJudith G Berman; Fiscal Year: 2012..CENs like those of higher organisms including humans, and unlike the well characterized point centromeres of Saccharomyces cerevisiae. Importantly, C...
- The development of probiotic yeast as an inexpensive vaccine delivery platformTRACEY JANE LAMB; Fiscal Year: 2013..Using genetic techniques already established for Saccharomyces cerevisiae I will genetically engineer S...
- Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogenMICHAEL THOMAS FASULLO; Fiscal Year: 2013..many DNA metabolism and housekeeping genes are conserved from yeast to man, high throughput analysis of Saccharomyces cerevisiae (budding yeast) genes that confer resistance to carcinogens have identified human genes that confer ..
- Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.Neil Hunter; Fiscal Year: 2012Crossing-over is essential for accurate chromosome segregation during meiosis. In Saccharomyces cerevisiae, crossing-over is specifically promoted by ScZip3, a putative E3-ligase for SUMO...
- Histoplasma Gene Expression and Organism PersistenceALAN GEORGE SMULIAN; Fiscal Year: 2012..of the gene encoding Orf 12k15 as an ACS by determining the activity of Orf 12k15 protein biochemically in Saccharomyces cerevisiae (Sc) and Hc and by functional complementation in Sc...
- Environmental and genetic regulation of copy number variation (CNV)THOMAS PETES; Fiscal Year: 2010..In this proposal, we describe the use of the yeast Saccharomyces cerevisiae as a model to find out how environmental contaminants and various mutations control the rate of ..
- Regulation of an Iron Import Facilitator by Transcriptional InterferenceCAMILLE REYES NERY; Fiscal Year: 2010..In Saccharomyces cerevisiae, genes encoding iron transporters and iron uptake facilitators are activated by the transcription ..
- George M Carman; Fiscal Year: 2016..roles of PAP in lipid metabolism became possible by our discoveries of the PAP-encoding genes from the yeast Saccharomyces cerevisiae (e.g., PAH1) and mammals (e.g., LPIN1)...
- The Roles of Trm9 and tRNA Methylation in the DNA Damage ResponseThomas J Begley; Fiscal Year: 2010..We will exploit three powerful model systems (based in Saccharomyces cerevisiae, humans, and mice) to test our hypothesis, to increase our understanding of translational ..
- Yeast Based Assays for Chemical Screens Against SARS-CoV TargetsDANIEL A contact ENGEL; Fiscal Year: 2010..The budding yeast Saccharomyces cerevisiae will be used as a living test tube to measure the effects of specific SARS-CoV proteins on growth...
- Joel M Goodman; Fiscal Year: 2016..Bakers yeast, Saccharomyces cerevisiae is a great system for this research, since the cells can robustly store lipid in droplets and the ..
- Bioactive lipids in food and pharmaceutical productsEric J Haas-Stapleton; Fiscal Year: 2013..Our preliminary studies show that Saccharomyces cerevisiae (brewers yeast), a saprophytic fungus, also produces PGE2. PGE2 generated by S...
- Nikon TiE Motorized Microscope System For 3D Image Acquisition, Deconvolution, LiRobert S Fuller; Fiscal Year: 2010..The Engelke lab is examining the three-dimensional organization of nuclear genomes in the budding yeast, Saccharomyces cerevisiae, and in murine and human cells...
- Role of chromatin structure in regulating gene silencingMary Bryk; Fiscal Year: 2009..of RNA polymerase II-transcribed genes located in the ribosomal DNA locus (rDNA) of the budding yeast Saccharomyces cerevisiae...
- Paul K Herman; Fiscal Year: 2016..This proposal addresses these broader issues by examining a model RNP complex, the P-body of the yeast, Saccharomyces cerevisiae...
- Anthony P Bretscher; Fiscal Year: 2016....
- Regulation of RNA SurveillanceMILES FROME WILKINSON; Fiscal Year: 2012..Current estimates from microarray analyses from several groups indicate that between 5 and 10% of the genes in Saccharomyces cerevisiae, Drosophila melanogaster, and mammals give rise to transcripts that are subject to NMD...
- MARIA E CARDENAS-CORONA; Fiscal Year: 2015..The central TORC1 members are the Tor protein kinases, which were discovered in the yeast Saccharomyces cerevisiae, a model system that has been crucial in elucidating the TORC1 signaling cascade...
- Identification of broad-spectrum antifungal efflux pump inhibitorsRichard Cannon; Fiscal Year: 2009..the University of New Mexico Center for Molecular Discovery (UNMCMD) and a panel of strains of the model yeast Saccharomyces cerevisiae, developed in our laboratory...
- Identifying novel small molecules for improved antifungal drug treatmentMartha L Bulyk; Fiscal Year: 2012..of this project is to identify small molecule inhibitors of PDR TFs involved in antifungal drug resistance in Saccharomyces cerevisiae as well as the pathogenic species Candida albicans and Candida glabrata...
- Drs2p Function in Clathrin-coated Vesicle BuddingTodd R Graham; Fiscal Year: 2013..Characterization of P4-ATPases in the budding yeast Saccharomyces cerevisiae (Drs2, Neo1, Dnf1, Dnf2 and Dnf3) has allowed the application of powerful molecular genetic tools ..
- Kinetochore Function and Cell Cycle Progression RevisionKatsumi Kitagawa; Fiscal Year: 2012..For these studies we will use the budding yeast Saccharomyces cerevisiae, as its process of mitotic division is comparable with that of multicellular eukaryotes...
- MARK W HOCHSTRASSER; Fiscal Year: 2016..The proposed research will concentrate on the yeast Saccharomyces cerevisiae because of its experimental advantages and the fact that the Ub system in general, and the ERAD ..
- RODNEY J ROTHSTEIN; Fiscal Year: 2014..control of these processes by studying the formation and disassembly of repair/recombination foci using Saccharomyces cerevisiae as a model system...
- Spinocerebellar ataxia 7 protein function.Patrick A Grant; Fiscal Year: 2010..are 2 homologous and highly conserved multi-subunit HAT complexes that were originally identified in Saccharomyces cerevisiae...
- Interaction Between DNA Repair and Antioxidant Genes During AgingCarlos A Torres Ramos; Fiscal Year: 2010..To test this hypothesis we will use two model organisms, a simple eukaryotic organism, the yeast Saccharomyces cerevisiae, and a more complex mammalian organism, a knockout mouse heterozygous for Ape1, the main AP ..
- Regulation of Replication Origin Usage in Saccharomyces cerevisiaeBik Kwoon Tye; Fiscal Year: 2009..Therefore, understanding the regulation of DNA replication is central to the study of the etiology of all genetic diseases rooted in genome instability including trisomy and cancer. ..
- Role of Set2 and H3 methylation in chromatin functionBrian D Strahl; Fiscal Year: 2013..Using Saccharomyces cerevisiae as a model organism, we plan to use a combination of biochemistry and genetics to further address ..
- GENETIC AND MOLECULAR ANALYSIS OF YEAST DNA REPLICATIONRobert A Sclafani; Fiscal Year: 2012..genetic analysis to identify the regulatory molecules of these processes using the single- celled eukaryote, Saccharomyces cerevisiae as a model system...
- Signaling iteractions in cytokinesis, cell wall biogenesis, and growth in fungiJose R Rodriguez-Medina; Fiscal Year: 2011..Myosin ll-deficient Saccharomyces cerevisiae cells (myolA) exhibit multiple phenotypes with characteristics of both cytokinesis and cell wall ..
- Hiten D Madhani; Fiscal Year: 2016..of genome-wide knockout collections has been instrumental in dissecting the biology of model fungi such as Saccharomyces cerevisiae and Schizosaccharomyces pombe, the generation of analogous complete gene deletion collections in ..
- Weiwei Dang; Fiscal Year: 2015Abstract Replicative aging of budding yeast, Saccharomyces cerevisiae, has been a remarkably useful model for aging studies, providing fundamental genetic and molecular insights into aging...
- Nils G Walter; Fiscal Year: 2015..Due to the availability of unique genetic and biochemical manipulation tools, the budding yeast Saccharomyces cerevisiae has long provided a central model system for dissecting the mechanism of eukaryotic pre-mRNA ..
- ROS sensing and signaling in lifespan regulationElizabeth A Schroeder; Fiscal Year: 2013..Using the yeast Saccharomyces cerevisiae as a model for post-mitotic cellular aging, we recently demonstrated that elevated levels of ..
- Mitochondrial aconitase: Fe-S cluster biogenesis and interaction with mtDNADebkumar Pain; Fiscal Year: 2012..We have discovered that isolated wild-type Saccharomyces cerevisiae mitochondria contain a nucleotide (GTP, NAD(P)H and ATP)- dependent machinery for iron-sulfur ..
- PROMOTER SPECIFICITY OF TRANSCRIPTION FACTORSDavid J Stillman; Fiscal Year: 2010..The yeast Saccharomyces cerevisiae with its powerful genetic tools has proven to be the model eukaryotic organism for studies of gene ..
- Defining the genomic architecture of expression quantitative traitsIAN MICHAEL EHRENREICH; Fiscal Year: 2010..In particular, a cross of the BY lab strain and the RM wine strain of the budding yeast Saccharomyces cerevisiae that was done by the lab of Leonid Kruglyak, where I am presently a postdoctoral fellow, has ..
- Effect of beta-glucan on sepsisCOURTNI NEWSOME; Fiscal Year: 2009..The soluble, highly purified beta-glucan PGG glucan, which was prepared from Saccharomyces cerevisiae, has been shown to prime antimicrobial functions, including increasing the oxidative burst response,..
- Christopher G Burd; Fiscal Year: 2014..so that cargo moves anterograde through the Golgi while Golgi residents are retained? Using budding yeast (Saccharomyces cerevisiae) to investigate sorting reactions in the Golgi, we discovered that a cytosolic protein, Vps74, ..
- Aaron M Neiman; Fiscal Year: 2016..The spore wall of Saccharomyces cerevisiae is a complex structure that forms de novo during the process of sporulation...
- THOMAS PETES; Fiscal Year: 2015..DNA damage induced at different points in the cell cycle? All of these issues will be addressed in the yeast Saccharomyces cerevisiae using a novel method for selecting crossovers, and microarrays capable of high-resolution (<1 kb)..
- Reducing genetic buffering to identify QTLs that affect cell shape and growthKerry A Geiler-Samerotte; Fiscal Year: 2013..We will perform QTL screening using Saccharomyces cerevisiae, a widely used model organism for researching the molecular basis of human disease...
- J Marie Hardwick; Fiscal Year: 2016..is to uncover the underlying mechanisms of KCTD7 based on novel insights gained from our studies in yeast (Saccharomyces cerevisiae), combined with mammalian models of neuronal cell death, mitochondrial function and autophagy...
- Regulation of Chromosome SegregationDAVID OWEN MORGAN; Fiscal Year: 2013..The proposed studies will explore the control of sister-chromatid separation in the budding yeast Saccharomyces cerevisiae, where much of our knowledge of this process was first uncovered...
- Carla M Koehler; Fiscal Year: 2016..the mechanism of protein import into the mitochondrion in the experimental model, the budding yeast Saccharomyces cerevisiae and to extend our studies into mammalian systems...
- David Gresham; Fiscal Year: 2016..We will use the single-celled eukaryotic microbes, Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) to identify the conserved networks ..
- David J Eide; Fiscal Year: 2016..e. the control of zinc levels within intracellular organelles. These processes will be studied using the yeast Saccharomyces cerevisiae as a model eukaryotic cell...
- Expanding The Genetic Code In YeastPaul R Copeland; Fiscal Year: 2013..Interestingly, however, neither of these unique amino acids are utilized by fungi, thus making the yeast Saccharomyces cerevisiae a genetically manipulable blank slate for reconstituting genetic code expansion...
- TRANSLATIONAL CONTROL OF YEAST MITOCHONDRIAL GENESTHOMAS DAVID FOX; Fiscal Year: 2013..The overall goal of this proposal is to exploit the model organism Saccharomyces cerevisiae to elucidate mechanisms, conserved in humans, that control expression and assembly of ..
- David Gius; Fiscal Year: 2015..this regard, the genes that play a critical role in longevity (or aging) have recently been characterized in Saccharomyces cerevisiae and C. elegans and the human homologs of these genes are referred to as the Sirtuin gene family...
- Echinocandin Reduced/Increased Susceptibility: MechanismSantosh Kumar Katiyar; Fiscal Year: 2013..To uncover this mechanism, two distinct genetic screens in Saccharomyces cerevisiae were employed, both implicating sphingolipid synthesis. Consequently, lipid analysis of C...
- Andreas Hochwagen; Fiscal Year: 2014..Meiotic DSB control will be investigated in the sexually reproducing yeast Saccharomyces cerevisiae...
- SUZANNE ELIZABETH BEREZOVSKY; Fiscal Year: 2014..Low doses of plumbagin induce tolerance to high doses in the budding yeast Saccharomyces cerevisiae, cause lifespan extension in the worm Caenorhabditis elegans, and protect mice against brain damage ..
- Feng Gong; Fiscal Year: 2014..How NER operates in the context of chromatin is largely unknown. Our previous studies in Saccharomyces cerevisiae first linked the prototype ATP-dependent chromatin remodeling complex SWI/SNF to NER...
- DNA damage-induced regulation of base excision repair by dynamic localizationNICHOLAS CHRISTOPHER BAUER; Fiscal Year: 2013..Since DNA repair and localization mechanisms are highly conserved, the powerful model system Saccharomyces cerevisiae is well-suited for studying both protein localization and DNA repair. An S...
- Antoni Barrientos; Fiscal Year: 2015..the proposed research is to investigate the players and mechanisms involved in COX biogenesis using the yeast Saccharomyces cerevisiae and cultured human cells as research models...
- Vesicle trafficking defects &mitochondrial dysfunction related to a-syn toxicityPAVAN KUMAR AULUCK; Fiscal Year: 2013..Overexpression of wildtype and mutant [unreadable]- synuclein in the budding yeast, Saccharomyces cerevisiae, has revealed strong dose-dependence for [unreadable]- synuclein toxicity, just as in the human ..
- Biosynthesis of Antifungal Lipopeptides from Filamentous FungiYi Tang; Fiscal Year: 2013..Using a combination of genetic knockout/knock-in, heterologous expression in Saccharomyces cerevisiae and in vitro biochemical interrogation, we will dissect the echinocandin NRPS (EcdA) and associated ..
- Membrane Protein Production Using the Yeast SPP SystemNANCY ANN WOYCHIK; Fiscal Year: 2013..This system, called the yeast SPP system, uses Saccharomyces cerevisiae and a bacterial toxin called MazF to impart a state of growth arrest that allows continued ..
- Rebecca W Heald; Fiscal Year: 2015..biochemical, genetic and state-of-the-art single molecule imaging approaches in the single cellular eukaryote Saccharomyces cerevisiae to address these three specific aims...
- Daniel J Lew; Fiscal Year: 2016..role of receptor endocytosis in modulating signaling activity and gradient sensing in the mating response of Saccharomyces cerevisiae (yeast). Yeast undergo a developmental decision based on the concentration of pheromone...
- Peter M Pryciak; Fiscal Year: 2016..This proposal uses the mating reaction of the yeast Saccharomyces cerevisiae as a model system for understanding eukaryotic signal transduction, using a molecular genetics and ..
- B Franklin Pugh; Fiscal Year: 2016..The model system is the budding yeast Saccharomyces cerevisiae, because of its experimental tractability and the mechanistic similarities to assembly of the ..
- Molecular Analysis of Multivesicular Body FormationCHARLES G ODORIZZI; Fiscal Year: 2013..Previous work on this project has identified mechanisms by which the Bro1 protein in Saccharomyces cerevisiae participates in the MVB cargo sorting pathway...
- RANDAL ARTHUR HALFMANN; Fiscal Year: 2015..In the budding yeast, Saccharomyces cerevisiae, multiple intrinsically disordered proteins (IDPs), including transcription factors, RNA- binding ..
- Benjamin S Glick; Fiscal Year: 2016..We propose to clarify the action of COPI in Saccharomyces cerevisiae. Our main approach is a refined version of the "anchor-away" method...
- Brian K Kennedy; Fiscal Year: 2016..complementary expertise have joined forces to develop a comprehensive understanding of replicative aging in Saccharomyces cerevisiae using a combination of high throughput and state-of-the-art approaches. Dr...
- John L Woolford; Fiscal Year: 2016..The long term goal of this project is to understand how ribosomes are assembled in eukaryotes. We use the yeast Saccharomyces cerevisiae, to facilitate combined genetic, molecular biological, biochemical, and proteomic approaches...
- Jeffry L Corden; Fiscal Year: 2016..We will continue to study the roles of Saccharomyces cerevisiae RNA-binding proteins Nrd1, Nab3 and the RNA helicase Sen1 in transcription termination...
- Eric M Phizicky; Fiscal Year: 2016..Work in this lab focuses on two major aspects of the biology of tRNA and its modifications in the yeast Saccharomyces cerevisiae...
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