Genomes and Genes
Schizosaccharomyces pombe 972h-
Publications125 found, 100 shown here
- Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assemblyJ Nakayama
Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
Science 292:110-3. 2001..These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly...
- Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domainA J Bannister
Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
Nature 410:120-4. 2001..This model may also explain the stable inheritance of the heterochromatic state...
- Global transcriptional responses of fission yeast to environmental stressDongrong Chen
The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
Mol Biol Cell 14:214-29. 2003..Promoter motifs associated with some of the groups of coregulated genes were identified. We compare and contrast global regulation of stress genes in fission and budding yeasts and discuss evolutionary implications...
- The fission yeast RNA binding protein Mmi1 regulates meiotic genes by controlling intron specific splicing and polyadenylation coupled RNA turnoverHuei Mei Chen
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, United States of America
PLoS ONE 6:e26804. 2011..Inactivation of Mmi1 in meiosis allows meiotic expression, through splicing and RNA stabilization, of at least 29 target genes, which are apparently constitutively transcribed...
- Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damageSteven L Sanders
The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Pathology, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
Cell 119:603-14. 2004..These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2...
- Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycleSophie G Martin
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Genopode Building, 1015 Lausanne, Switzerland
Nature 459:852-6. 2009..In short cells, Pom1 prevents precocious M-phase entry, suggesting that the higher medial Pom1 levels inhibit Cdr2 and promote a G2 delay. Thus, gradients of Pom1 from cell ends provide a measure of cell length to regulate M-phase entry...
- Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell divisionB Feierbach
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
Curr Biol 11:1656-65. 2001..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons...
- The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeastKentaro Nakano
Division of Biology, Department of Life Sciences, Graduate Program of Arts and Sciences, University of Tokyo, Tokyo 153 8902, Japan
J Cell Sci 115:4629-39. 2002..In conclusion, we propose that the Rho3-For3 signaling system functions in the polarized cell growth of fission yeast by controlling both actin cytoskeleton and MTs...
- Homologous recombination restarts blocked replication forks at the expense of genome rearrangements by template exchangeSarah Lambert
Institut Curie Centre National de la Recherche Scientifique, UMR3348, Réponse Cellulaire aux Perturbations de la Réplication, Universite Paris Sud XI, Orsay, France
Mol Cell 39:346-59. 2010..In contrast, we report that the Srs2 helicase promotes both fork restart and template exchange. Our data demonstrate that template exchange occurs during recombination-dependent fork restart at the expense of genome rearrangements...
- Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domainReza Saberianfar
Department of Biology, University of Western Ontario, London, Ontario, Canada
PLoS ONE 6:e24694. 2011..Significantly, genetic analysis of lsk1Δ aip1Δ double mutants is consistent with Lsk1p and Aip1p acting in a linear pathway with respect to the regulation of cytokinesis...
- Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomesYuji Chikashige
Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588 2 Iwaoka, Iwaoka cho, Nishi ku, Kobe 651 2492, Japan
Cell 125:59-69. 2006..Significantly, when both Bqt1 and Bqt2 are ectopically expressed in mitotic cells, they also form a bridge between Rap1 and Sad1. Thus, a complex including Bqt1 and Bqt2 is essential for connecting telomeres to the SPB...
- The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathwayT Toda
Cell Regulation Laboratory, Imperial Cancer Research Fund, London, United Kingdom
Mol Cell Biol 16:6752-64. 1996..pombe, Pmk1 may not act in a linear manner with respect to fission yeast protein kinase C homologs. Interestingly, however, these two pathways are not independent; instead, they regulate cell integrity in a coordinate manner...
- The transcriptional program of meiosis and sporulation in fission yeastJuan Mata
The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, UK
Nat Genet 32:143-7. 2002....
- Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAiThomas A Volpe
Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
Science 297:1833-7. 2002..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
- RNA polymerase II is required for RNAi-dependent heterochromatin assemblyHiroaki Kato
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
Science 309:467-9. 2005..This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly...
- Dicer-independent primal RNAs trigger RNAi and heterochromatin formationMario Halic
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Cell 140:504-16. 2010..Our results suggest that a transcriptome surveillance mechanism based on random association of RNA degradation products with Argonaute triggers siRNA amplification and heterochromatin assembly within DNA repeats...
- Topoisomerase I regulates open chromatin and controls gene expression in vivoMickaël Durand-Dubief
Department of Biosciences and Nutrition, Center for Biosciences, Karolinska Institutet, Novum, Huddinge, Sweden
EMBO J 29:2126-34. 2010..Our study links topoisomerase activity to the maintenance of open chromatin and regulating transcription in vivo...
- RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formationMartin Zofall
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Science 335:96-100. 2012..Our analyses uncover unexpected regulatory roles for mRNA-processing factors that assemble dynamic heterochromatin to modulate gene expression...
- Role of the Small GTPase Rho3 in Golgi/Endosome trafficking through functional interaction with adaptin in Fission YeastAyako Kita
Laboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kinki University, Higashi Osaka, Japan
PLoS ONE 6:e16842. 2011....
- PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localizationK Nakai
Institute for Molecular and Cellular Biology, Osaka University, Japan
Trends Biochem Sci 24:34-6. 1999
- Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4 and a genome-wide search for its target genesH Abe
Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi ku, Osaka 558 8585, Japan
Genetics 154:1497-508. 2000..Furthermore, expression of a reporter gene driven by the authentic mei4 promoter was induced in vegetative cells by ectopic overproduction of Mei4p. These results suggest that mei4 transcription is positively autoregulated...
- Telomere-led bouquet formation facilitates homologous chromosome pairing and restricts ectopic interaction in fission yeast meiosisO Niwa
Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
EMBO J 19:3831-40. 2000..Thus, telomere-led chromosome organization facilitates homologous pairing and also restricts irregular chromosome pairing during meiosis...
- Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombeR J Pelham
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Nat Cell Biol 3:235-44. 2001..Our studies give rise to a model in which actin patches are centres for actin polymerization that drive their own movement on actin cables using Arp2/3-based actin polymerization...
- Wsh3/Tea4 is a novel cell-end factor essential for bipolar distribution of Tea1 and protects cell polarity under environmental stress in S. pombeHisashi Tatebe
Section of Microbiology, Division of Biological Sciences, University of California, Davis, Davis, California 95616, USA
Curr Biol 15:1006-15. 2005..The deltatea1 mutant cells cannot grow in a bipolar fashion and show T-shaped morphology after heat shock...
- Cdc42 regulates multiple membrane traffic events in fission yeastMiguel Estravís
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas CSIC Departamento de Microbiología y Genética, Universidad de Salamanca, 30007 Salamanca, Spain
Traffic 12:1744-58. 2011..All together, we propose that Cdc42 and the scaffold Pob1 are required for membrane trafficking and fusion, contributing to polarized secretion, endosome recycling, vacuole formation and growth...
- Conserved function of RNF4 family proteins in eukaryotes: targeting a ubiquitin ligase to SUMOylated proteinsHuaiyu Sun
Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
EMBO J 26:4102-12. 2007..Hence, we describe a family of SIM-containing RING-finger proteins that potentially regulates eukaryotic genome stability through linking SUMO-interaction with ubiquitin conjugation...
- Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeastAyako Kita
Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
Mol Biol Cell 15:2920-31. 2004....
- Selective elimination of messenger RNA prevents an incidence of untimely meiosisYuriko Harigaya
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
Nature 442:45-50. 2006..We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts...
- Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycleF Verde
Imperial Cancer Research Fund, London, United Kingdom
J Cell Biol 131:1529-38. 1995....
- RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencingIngela Djupedal
Karolinska Institutet, Department of Biosciences, School of Life Sciences, University College Sodertorn, S 141 89 Huddinge, Sweden
Genes Dev 19:2301-6. 2005..In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing...
- SUMO-targeted ubiquitin ligases in genome stabilityJohn Prudden
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
EMBO J 26:4089-101. 2007..The DNA repair factor Rad60 and its human homolog NIP45, which contain SLDs, are candidate STUbL targets. Consistently, Rad60 and Slx8-Rfp mutants have similar DNA repair defects...
- Transcriptional regulatory network for sexual differentiation in fission yeastJuan Mata
Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1HH, UK
Genome Biol 8:R217. 2007..The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes...
- Two conserved modules of Schizosaccharomyces pombe Mediator regulate distinct cellular pathwaysTomas Linder
Division of Metabolic Diseases, Karolinska Institutet, Novum, SE 141 86 Stockholm, Sweden
Nucleic Acids Res 36:2489-504. 2008..Comparison with Saccharomyces cerevisiae Mediator expression data reveals that these functionally distinct modules are conserved between S. pombe and S. cerevisiae...
- Compartmentalized signaling of Ras in fission yeastBrian Onken
Department of Molecular and Cell Biology, The Breast Center, Baylor College of Medicine, 1 Baylor Plaza, BCM 600, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 103:9045-50. 2006..These observations provide a striking demonstration of compartment-specific Ras signaling and indicate that spatial specificity in the Ras pathway is evolutionarily conserved...
- Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesisOlivier Hachet
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
Genes Dev 22:3205-16. 2008..Taken together, these data establish that Mid1p and the SIN are the key regulators that orchestrate CAR assembly...
- Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeastYan Ma
Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
Genes Cells 14:1015-28. 2009..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
- Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeastTomoyasu Sugiyama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
EMBO J 30:1027-39. 2011..These results demonstrate that Red1 is involved in DSR-directed RNA decay to prevent ectopic expression of meiotic mRNAs in vegetative cells...
- Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1Gregory A Cope
Department of Biology, California Institute of Technology Caltech, Pasadena, CA 91125, USA
Science 298:608-11. 2002..We propose that JAMM isopeptidases play important roles in a variety of physiological pathways...
- Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual developmentA Sugimoto
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan
Genes Dev 5:1990-9. 1991..These observations strongly suggest that Ste11 serves as a key transcription factor for sexual development...
- The Schizosaccharomyces pombe mei4+ gene encodes a meiosis-specific transcription factor containing a forkhead DNA-binding domainS Horie
Department of Biology, Faculty of Science, Osaka City University, Osaka, Japan
Mol Cell Biol 18:2118-29. 1998..These results indicate that Mei4 functions as a meiosis-specific transcription factor of S. pombe...
- The endo-beta-1,3-glucanase eng1p is required for dissolution of the primary septum during cell separation in Schizosaccharomyces pombeAna Belén Martín-Cuadrado
Instituto de Microbiologia Bioquimica, Departamento de Microbiologia y Genetica, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain
J Cell Sci 116:1689-98. 2003..Thus, ace2p may regulate the expression of the eng1+ gene together with that of other genes whose products are also involved in cell separation...
- Rho3p regulates cell separation by modulating exocyst function in Schizosaccharomyces pombeHongyan Wang
Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Republic of Singapore
Genetics 164:1323-31. 2003..Taken together, our results suggest the possibility that S. pombe Rho3p regulates cell separation by modulation of exocyst function...
- The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Schizosaccharomyces pombeBalazs Ribar
Sealy Center for Molecular Science, University of Texas Medical Branch, 6 148 Medical Research Building, Galveston, TX 77555, USA
Nucleic Acids Res 32:115-26. 2004..We confirmed the presence of APE activity in recombinant Apn2 and in crude cell extracts. Thus S.pombe is distinct from S.cerevisiae, and is similar to mammalian cells in having Apn2 as the major APE...
- RNase-sensitive DNA modification(s) initiates S. pombe mating-type switchingSonya Vengrova
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
Genes Dev 18:794-804. 2004..We propose a model in which a swi1- and swi3-dependent signal during lagging-strand synthesis leads to pausing of leading-strand replication and the introduction of the imprint...
- Constitutive activation of the fission yeast pheromone-responsive pathway induces ectopic meiosis and reveals ste11 as a mitogen-activated protein kinase targetSøren Kjaerulff
Institute of Molecular Biology, University of Copenhagen, Øster Farimagsgade 2A, DK 1353 Copenhagen K, Denmark
Mol Cell Biol 25:2045-59. 2005....
- RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombeAlison B Ansbach
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
Mol Biol Cell 19:595-607. 2008..We propose that RFC(Ctf18) and the Swi1-Swi3 complex function in separate and redundant pathways essential for replication fork stabilization to facilitate sister chromatid cohesion in fission yeast...
- A complex gene regulatory mechanism that operates at the nexus of multiple RNA processing decisionsDavid S McPheeters
Center for RNA Molecular Biology and Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, Ohio 44106 4960, USA
Nat Struct Mol Biol 16:255-64. 2009..This highly integrated regulatory strategy may ensure a rapid response to adverse conditions, thereby guaranteeing survival...
- A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damageJanette Lloyd
Division of Molecular Structure, Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
Cell 139:100-11. 2009..Finally, we establish that human Nbs1 interactions with Mdc1 occur through both its FHA- and BRCT-repeat domains, suggesting how their structural and functional interdependence underpins Nbs1 adaptor functions in the DNA-damage response...
- The nuclear poly(A)-binding protein interacts with the exosome to promote synthesis of noncoding small nucleolar RNAsJean François Lemay
RNA Group, Department of Biochemistry, Universite de Sherbrooke, Sherbrooke, QC JIH 5N4, Canada
Mol Cell 37:34-45. 2010..This work unveils a function for the nuclear PABP in snoRNA synthesis and provides insights into exosome recruitment to polyadenylated RNAs...
- Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cellsSoichiro Yamanaka
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo, Japan
EMBO J 29:2173-81. 2010..These patches may represent the sites for degradation of meiosis-specific mRNAs with untimely expression...
- Negative regulation of meiotic gene expression by the nuclear poly(a)-binding protein in fission yeastOlivier St-André
RNA Group, Universite de Sherbrooke, Department of Biochemistry, Sherbrooke, Quebec J1H 5N4, Canada
J Biol Chem 285:27859-68. 2010..Our findings have therefore uncovered a mode of gene regulation whereby a poly(A)-binding protein promotes RNA degradation in the nucleus to prevent untimely expression...
- Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeastFelipe O Bendezú
Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH 1015 Lausanne, Switzerland
Mol Biol Cell 22:44-53. 2011..Thus in fission yeast long-range cytoskeletal transport and PIP₂-dependent exocyst represent parallel morphogenetic modules downstream of Cdc42, raising the possibility of similar mechanisms in other cell types...
- Genetic analysis of cell morphogenesis in fission yeast--a role for casein kinase II in the establishment of polarized growthV Snell
ICRF Cell Cycle Laboratory, Imperial Cancer Research Fund, London, UK
EMBO J 13:2066-74. 1994..orb5 encodes a fission yeast homologue of casein kinase II alpha. We propose that this kinase plays a role in the translation of cell polarity into polarized growth, but not in the establishment of polarity itself...
- Localization of the (1,3)beta-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germinationJuan Carlos G Cortés
Departamento de Microbiologia y Genetica, Consejo Superior de Investigaciones Científicas CSIC Universidad de Salamanca, 37007 Salamanca, Spain
J Cell Sci 115:4081-96. 2002....
- Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1Kanji Furuya
Genome Damage and Stability Centre, University of Sussex, Brighton, BN1 9RQ, UK
Genes Dev 18:1154-64. 2004..When the Rad9-T412/S423 are phosphorylated, Rad4(TOPBP1) coprecipitates with Rad3(ATR), suggesting that phosphorylation coordinates formation of an active checkpoint complex...
- Activation of the pheromone-responsive MAP kinase drives haploid cells to undergo ectopic meiosis with normal telomere clustering and sister chromatid segregation in fission yeastTakaharu G Yamamoto
Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communication Technology, 588 2 Iwaoka cho, Iwaoka, Nishi ku, Kobe 651 2492, Japan
J Cell Sci 117:3875-86. 2004..In contrast, in meiosis induced by inactivation of Pat1, sister chromatids segregate precociously during the first meiotic division. Thus, these results suggest that activation of Spk1 drives meiosis in S. pombe...
- Synthesis of alpha-glucans in fission yeast spores is carried out by three alpha-glucan synthase paralogues, Mok12p, Mok13p and Mok14pIgnacio Garcia
Instituto de Microbiologia Bioquimica, CSIC Universidad de Salamanca and Departamento de Microbiología y Genética, Universidad de Salamanca Campus Miguel de Unamuno 37007 Salamanca, Spain
Mol Microbiol 59:836-53. 2006....
- Protein complexes at the microtubule organizing center regulate bipolar spindle assemblyAdrianna S Rodriguez
Department of Medicine, NYU School of Medicine, New York, New York, USA
Cell Cycle 7:1246-53. 2008..Our findings provide novel insights into regulation of the bipolar mechanism through the MTOC complex...
- High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis ICornelia Rumpf
Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria
Cell Cycle 9:1802-8. 2010..Using this approach, we identified a new protein, Dil1, which is required to prevent meiosis I homolog non-disjunction. We show that Dil1 acts in the dynein pathway to promote oscillatory nuclear movement during meiosis...
- Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosisHilary A Snaith
Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Swann Building, Mayfield Road, Edinburgh EH93JR, UK
J Cell Sci 124:2187-99. 2011..We propose that Mug33 contributes to exocyst function and that actin cable-dependent vesicle transport and exocyst function have complementary roles in promoting efficient exocytosis in fission yeast...
- Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombeMiriam Sansó
Oxidative Stress and Cell Cycle Group, Departament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, C Dr Aiguader 88, 08003 Barcelona, Spain
Mol Microbiol 82:542-54. 2011....
- A central coupler for recombination initiation linking chromosome architecture to S phase checkpointTomoichiro Miyoshi
Department of Life Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Mol Cell 47:722-33. 2012..These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint...
- Mediator promotes CENP-a incorporation at fission yeast centromeresJonas O Carlsten
Institute of Biomedicine, University of Gothenburg, Goteborg, Sweden
Mol Cell Biol 32:4035-43. 2012..Our data thus identify Mediator as a crucial regulator of ncRNA transcription at fission yeast centromeres and add another crucial layer of regulation to centromere function...
- Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeastsM Nagy
Laboratoire d Enzymologie, Centre National de la Recherche Scientifique, Gif sur Yvette, France
Proc Natl Acad Sci U S A 89:8966-70. 1992..cerevisiae uses fumarate as terminal electron acceptor. These results are discussed in relation to the anaerobic growth competence of the two yeasts and to the fermentative processes they use...
- Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1T Toda
Department of Biophysics, Faculty of Science, Kyoto University, Japan
Mol Cell Biol 12:5474-84. 1992..These results suggest the functional importance of chromosome structure on the regulation of gene expression through the pap1 transcription factor...
- A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeastS A Nadin-Davis
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario
EMBO J 7:985-93. 1988..byr1 is thus another important gene in the sexual differentiation pathway and we believe that at least part of ras1 function is to act directly or indirectly through byr1 to modulate protein phosphorylation...
- Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeastJ B Millar
Division of Yeast Genetics, National Institute for Medical Research, Ridgeway, London, UK
Genes Dev 9:2117-30. 1995..We conclude that Pyp1 and Pyp2 are tyrosine-specific MAP kinase phosphatases that inactivate an osmoregulated MAP kinase, Sty1, which acts downstream of the Wis1 MAP kinase kinase to control cell size at division in fission yeast...
- Region-specific activators of meiotic recombination in Schizosaccharomyces pombeL C DeVeaux
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Genes Dev 8:203-10. 1994..Similar ade6 regional specificities were observed for rec8 and rec11. We infer that there are multiple activators of meiotic recombination, each specific for a limited set of loci, and we discuss how these regional activators may work...
- As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombeM Lollier
Laboratoire de Microbiologie et de Genetique, URA n 1481 Université Louis Pasteur CNRS, Strasbourg, France
Curr Genet 28:138-49. 1995..They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product...
- A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistanceT Usui
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan
Gene 189:43-7. 1997..These results suggest that the sks2+ gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state...
- A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeastY Adachi
Department of Biophysics, Faculty of Science, Kyoto University, Japan
Genes Cells 2:467-79. 1997..However, the precise molecular functions of MCM proteins are not yet clear...
- Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesisD Hirata
Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, Higashi Hiroshima 739, Japan
J Cell Sci 111:149-59. 1998..Analysis of functional overlapping between Rho2 and Rho1 revealed that their functions are distinct from each other, with partial overlapping...
- Cdm1, the smallest subunit of DNA polymerase d in the fission yeast Schizosaccharomyces pombe, is non-essential for growth and divisionN Reynolds
Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
Curr Genet 34:250-8. 1998..In addition, over-expression of cdm1+ suppresses the temperature-sensitive cdc24-M38 mutant suggesting that cdc24+ may also have a role in DNA polymerase delta function...
- A fission yeast gene (prr1(+)) that encodes a response regulator implicated in oxidative stress responseR Ohmiya
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa ku, Nagoya, 464 8601, Japan
J Biochem 125:1061-6. 1999..g., trr1(+), ctt1(+)), which are induced by oxidative stress. These results suggest that a His-Asp phosphorelay system may be involved in a stress-activated signaling pathway in S. pombe...
- A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damageT Takeda
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
Mol Cell Biol 19:5535-47. 1999..Our results reveal a novel function of the Cdc7/Dbf4-related kinase complex in S-phase checkpoint control as well as in growth recovery from DNA damage in addition to its predicted essential function in S-phase initiation...
- Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpointF Esashi
CREST Research Project, Department of Biophysics, Graduate School of Science, Kyoto University, Japan
Mol Cell 4:167-74. 1999..The T215 site is in vitro phosphorylated by purified Cdc2 kinase. Phosphorylation of T215 occurs intensely in response to DNA damage at a late stage, suggesting an antagonistic role of Cdc2 phosphorylation toward checkpoint...
- Isolation and characterization of par1(+) and par2(+): two Schizosaccharomyces pombe genes encoding B' subunits of protein phosphatase 2AW Jiang
Department of Biology, Syracuse University, Syracuse, New York 13244, USA
Genetics 154:1025-38. 2000..cerevisiae homolog Rts1p. Thus, while Schizosaccharomyces pombe is more similar to higher eukaryotes with respect to its complement of B'-encoding genes, the function of those proteins is conserved relative to that of Rts1p...
- Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivatedS W Wang
Imperial Cancer Research Fund Molecular Oncology Laboratory, University of Oxford Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
Mol Cell Biol 20:3234-44. 2000..Genetic data suggest that Cid1 acts in association with Crb2/Rhp9 and through the checkpoint-signaling kinase Chk1 to inhibit unscheduled mitosis specifically when DNA polymerase delta or epsilon is inhibited...
- Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromisedC R Wilkinson
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, Scotland, United Kingdom
J Biol Chem 275:15182-92. 2000..The Mts3 protein was unable to bind to polyubiquitin in vitro, but the Pus1 and Mts3 proteins were found to bind to one another in vitro, which taken together with the genetic data suggests that they are also closely associated in vivo...
- Schizosaccharomyces pombe ehs1p is involved in maintaining cell wall integrity and in calcium uptakeE Carnero
Departamento de Microbiologia y Genetica, Instituto de Microbiologia Bioquimica, CSIC and Universidad de Salamanca, Spain
Mol Gen Genet 264:173-83. 2000..Our results suggest that in fission yeast ehs1p forms part of a calcium channel that is involved in the cell wall integrity pathway that includes the kinase pck2p...
- Roles of the mitotic inhibitors Wee1 and Mik1 in the G(2) DNA damage and replication checkpointsN Rhind
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Cell Biol 21:1499-508. 2001..Mik1 appears to have two roles in the DNA damage checkpoint; one in the establishment of the checkpoint and another in its maintenance. In contrast, Wee1 does not appear to be involved in the establishment of either checkpoint...
- The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombeK Tanaka
School of Biological Sciences, 2 205 Stopford Building, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK
EMBO J 20:1259-70. 2001..The ability of plo1(+) overexpression to induce septation was severely compromised by SIN inactivation. We propose that Plo1 acts before the SIN to control septation...
- Fep1, an iron sensor regulating iron transporter gene expression in Schizosaccharomyces pombeBenoit Pelletier
Departement de Biochimie, Universite de Sherbrooke, Sherbrooke, J1H 5N4, Canada
J Biol Chem 277:22950-8. 2002..This suggests that in the presence of iron and Fep1, the Tup11 and Tup12 proteins may act as co-repressors for down-regulation of genes encoding components of the reductive iron transport machinery...
- A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathwayMaria A Marchetti
Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263 0001, USA
Proc Natl Acad Sci U S A 99:7472-7. 2002..These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage...
- Actin dynamics in the contractile ring during cytokinesis in fission yeastRobert J Pelham
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Nature 419:82-6. 2002..Our studies show that cytokinesis is a more dynamic process than previously thought and provide a perspective on the mechanism of cell division...
- Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasomeHsueh Chi S Yen
Department of Molecular and Cellular Biology, Breast Cancer Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
Cell 112:207-17. 2003..We propose that human Int6, either alone or cooperatively with Ras, influences proteasome activities via Rpn5. Inactivating Int6 can lead to accumulation of mitotic regulators affecting cell division and mitotic fidelity...
- The telomere protein Taz1 is required to prevent and repair genomic DNA breaksKyle M Miller
Telomere Biology Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, WC2A 3PX, London, United Kingdom
Mol Cell 11:303-13. 2003..Furthermore, hypersensitivity to treatments that induce DSBs suggests that Taz1p is involved in DSB repair. Our observations imply roles for Taz1p-containing telomeres in preventing and repairing DNA breaks throughout the genome...
- The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin functionStefanie Wesche
ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
Curr Biol 13:715-24. 2003..The Saccharomyces cerevisae UCS domain protein She4p is involved in two myosin-requiring events, endocytosis and mRNA localization...
- RNAi-mediated targeting of heterochromatin by the RITS complexAndre Verdel
Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
Science 303:672-6. 2004..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
- Fission yeast Arp6 is required for telomere silencing, but functions independently of Swi6Masaru Ueno
Department of Chemistry, Shizuoka University, 836 Oya, Shizuoka 422 8529, Japan
Nucleic Acids Res 32:736-41. 2004..These results suggest that Arp6 and Swi6 function independently at telomere ends. We propose that the Arp6-mediated repression mechanism works side by side with Swi6-based telomere silencing in S.pombe...
- The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligaseTomoko Andoh
Department of Biological Science, Faculty of Science, Kumamoto University, Kurokami, Kumamoto 860 8555, Japan
Biochem Biophys Res Commun 317:1138-43. 2004..We also isolated a multi-copy suppressor for ptr1-1 and found that it is the mpd2+ gene isolated as a multi-copy suppressor of cdc7-PD1...
- The Schizosaccharomyces pombe HIRA-like protein Hip1 is required for the periodic expression of histone genes and contributes to the function of complex centromeresChris Blackwell
Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle NE2 4HH, United Kingdom
Mol Cell Biol 24:4309-20. 2004..Thus, S. pombe Hir proteins contribute to pericentromeric heterochromatin, and our data thus suggest that Hir proteins may be required for the function of metazoan centromeres...
- Cds1 phosphorylation by Rad3-Rad26 kinase is mediated by forkhead-associated domain interaction with Mrc1Katsunori Tanaka
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Sciences, Shimane University, 1060 Nishikawatsu, Matsue 690 8504, Japan
J Biol Chem 279:32079-86. 2004..This interaction mediates the initial Thr-11 phosphorylation of Cds1 by Rad3-Rad26 with subsequent intermolecular phosphorylation events leading to full activation of Cds1...
- Role of the alpha-glucanase Agn1p in fission-yeast cell separationNick Dekker
Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Mol Biol Cell 15:3903-14. 2004..We propose that Agn1p acts in concert with Eng1p to achieve efficient cell separation, thereby exposing the secondary septa as the new ends of the daughter cells...
- Homo-oligomerization is the essential function of the tandem BRCT domains in the checkpoint protein Crb2Li Lin Du
Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 279:38409-14. 2004..These results demonstrate that the crucial function of the tandem BRCT domains is to oligomerize Crb2...
- swi1- and swi3-dependent and independent replication fork arrest at the ribosomal DNA of Schizosaccharomyces pombeGregor Krings
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
Proc Natl Acad Sci U S A 101:14085-90. 2004..The results support the notion that Swi1p and Swi3p act at several different protein-DNA complexes in the rDNA spacer regions to arrest replication but that not all fork barriers required their activity to arrest forks...
- A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1Chikashi Obuse
Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8501, Japan
Nat Cell Biol 6:1135-41. 2004..Double HP1 RNAi abolishes kinetochore localization of hMis12 and DC8. Therefore, centromeric HP1 may be the base to anchor the hMis12 core complex that is enriched with coiled coils and extends to outer Zwint-1 during mitosis...
- The roles of fission yeast ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signalingAkira Yamashita
Molecular Genetics Research Laboratory, Graduate School of Science, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
Mol Biol Cell 16:1378-95. 2005..Ase1, therefore, couples anaphase completion with cytokinesis upon cell division...
- Evolutionary-conserved telomere-linked helicase genes of fission yeast are repressed by silencing factors, RNAi components and the telomere-binding protein Taz1Klavs R Hansen
Department of Genetics, Institute of Molecular Biology and Physiology, University of Copenhagen, Denmark
Nucleic Acids Res 34:78-88. 2006..The tlh genes are found in the subtelomeric regions of several other fungi raising the intriguing possibility of conserved regulation and function...
- Rho2 is a target of the farnesyltransferase Cpp1 and acts upstream of Pmk1 mitogen-activated protein kinase signaling in fission yeastYan Ma
Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
Mol Biol Cell 17:5028-37. 2006..Together, our results demonstrate that Cpp1 is a key component of the Pck2-Pmk1 signaling through the spatial control of the small GTPase Rho2...
- Crystal structures of the adenylate sensor from fission yeast AMP-activated protein kinaseRobert Townley
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
Science 315:1726-9. 2007..Unexpectedly, ATP binds without counterions, amplifying its electrostatic effects on a critical regulatory region where all three subunits converge...
- CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEASTShelley Sazer; Fiscal Year: 2002..Test the hypothesis that the nuclear envelope is a downstream target of the spi1 GTPase system. ..
- REGULATION OF MITOSIS IN FISSION YEASTPaul Russell; Fiscal Year: 2003..The aim of these experiments will be to understand how activation of nuclear Cdc2/cyclin-B is coordinated with signals that indicate cell size and cell physiology that are presumed to emanate from cytoplasm. ..
- REGULATION OF MEIOTIC DEVELOPMENT BY MTS1-MTS2 PROTEINWAYNE WAHLS; Fiscal Year: 2004..4) To test alternative hypotheses that the Mts1-Mts2-M26 complex either enhances the use of a pre-existing recombinational initiation site or creates a new initiation site. The PI request four years of support to carry out this study. ..
- Combinatoial CREB/ATF dimers and cellular growth controlWAYNE WAHLS; Fiscal Year: 2004..Aim 3, To further characterize regulatory mechanisms for stress responses mediated by the Mts1 bZIP protein: elucidate the role of the MAP kinase Spcl in controlling an autoinhibitory function of Mts1. ..
- MOLECULAR ANALYSIS OF HOTSPOTS OF GENETIC RECOMBINATIONGerald Smith; Fiscal Year: 2007..Thus, the basic research proposed here will add to the foundations for understanding, diagnosing, preventing, and curing human disease. ..
- Characterization of the S. Pombe cAMP PathwayCHARLES HOFFMAN; Fiscal Year: 2005..We propose to continue our work on adenylate cyclase activation by conducting genetic, molecular and biochemical studies of the fission yeast glucose/cAMP pathway. ..
- An in vivo screen for biological and chemical regulators of mammalial PDEsCHARLES HOFFMAN; Fiscal Year: 2008..abstract_text> ..
- MECHANISTIC STUDIES OF DNA MISMATCH REPAIRA Lien Lu Chang; Fiscal Year: 2006..Particularly, alterations in protein-protein interactions under oxidative stress will be investigated. These studies should advance our understanding of the role of DNA repair in genome stability and tumor susceptibility. ..
- Nuclear envelope membrane proteins and nuclear structureMegan King; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- ZFN-driven Gene Disruption of CCR5 as a Potential Treatment of AIDSFyodor Urnov; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- DNA replication checkpoints in fission yeastGENNARO D URSO; Fiscal Year: 2007..Moreover, our results will likely identify new drug targets for cancer therapy, and reveal potentially less mutagenic strategies to block cell cycle progression in cancer cells. ..
- Repair of Oxidatively Damaged GuaninesA Lien Lu Chang; Fiscal Year: 2008..We anticipate that these studies will advance our understanding of carcinogenesis process and form the background work for the development of new anti-cancer drugs. ..
- REGULATION OF THE ONSET OF ANAPHASETomohiro Matsumoto; Fiscal Year: 2001....
- Structural investigation of Arp2/3 complex activationBradley Nolen; Fiscal Year: 2006..Furthermore, it will show how nucleation promoting factors activate the complex. This will provide a basis for a better understanding of cytoskeletal function in both normal and diseased states of the cell. ..
- Structure Determination of the Polymerase alpha-primaseDEWIGHT WILLIAMS; Fiscal Year: 2006..abstract_text> ..
- Role of Inositol Polyphosphates in Kinetochore Function and TranscriptionAles Vancura; Fiscal Year: 2006..The results of this work will ultimately contribute to identification of novel molecular targets for cancer therapy. ..
- Using Enhanced Gene Targeting to Repair the Sickle CellFyodor Urnov; Fiscal Year: 2004..While this study focuses on SCD, this approach represents a dramatic advance for the repair of any genetic point lesion. ..
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