Genomes and Genes
Salmonella typhimurium LT2
Alias: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, Salmonella enterica subsp. enterica serovar Typhimurium LT2, Salmonella enterica subsp. enterica serovar Typhimurium strain LT2, Salmonella enterica subsp. enterica serovar Typhimurium strain LT2-LTL2
Publications264 found, 100 shown here
- Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survivalD M Cirillo
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305, USA
Mol Microbiol 30:175-88. 1998..We conclude that SPI-2 genes are specifically expressed upon entry into mammalian cells and are required for intracellular growth in host cells in vivo and in vitro...
- Identification of a pathogenicity island required for Salmonella survival in host cellsH Ochman
Department of Biology, University of Rochester, NY 14627, USA
Proc Natl Acad Sci U S A 93:7800-4. 1996..The "pathogenicity island" harboring the spi genes may encode the virulence determinants that set Salmonella apart from other enteric pathogens...
- Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cellS Stender
Max von Pettenkofer Institut, Munich, Germany
Mol Microbiol 36:1206-21. 2000..In conclusion, as a result of SopE2 all S. typhimurium strains tested have the capacity to activate Cdc42 signalling inside host cells which is important to ensure efficient entry...
- A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K-12 chromosomeD M Mills
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Microbiol 15:749-59. 1995..coli K-12 chromosome and may represent a 'pathogenicity island'. We speculate that acquisition of the 40 kb region must have significantly advanced the evolution of Salmonella as a pathogen...
- Ethanolamine utilization in Salmonella typhimurium: nucleotide sequence, protein expression, and mutational analysis of the cchA cchB eutE eutJ eutG eutH gene clusterI Stojiljkovic
Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201
J Bacteriol 177:1357-66. 1995..Ethanolamine-utilization mutants were slightly attenuated in a mouse model of S. typhimurium infection, indicating that ethanolamine may be an important source of nitrogen and carbon for S. typhimurium in vivo...
- PurF-independent phosphoribosyl amine formation in yjgF mutants of Salmonella enterica utilizes the tryptophan biosynthetic enzyme complex anthranilate synthase-phosphoribosyltransferaseBeth Ann Browne
Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Room 120, Madison, WI 53706, USA
J Bacteriol 188:6786-92. 2006..The data presented here are consistent with a model in which the absence of YjgF uncovers a new catalytic activity of TrpDE...
- Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-component regulatory system with a possible role in virulenceK L Roland
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322
J Bacteriol 175:4154-64. 1993We isolated spontaneous mutations (pmrA) in the smooth strain Salmonella typhimurium LT2 that show increased resistance to the cationic antibacterial proteins of human neutrophils and to the drug polymyxin B...
- Crucial roles of both flanking sequences in silencing of the hilA promoter in Salmonella entericaIgor N Olekhnovich
Department of Microbiology, University of Virginia School of Medicine, Charlottesville, VA 22908 0734, USA
J Mol Biol 357:373-86. 2006..Action of activators requires contact with RNA polymerase from their DNA binding site, centered at position -77, relative to the hilA transcription start site...
- Salmonella enterica serovar typhimurium pathogenicity island 1-encoded type III secretion system translocases mediate intimate attachment to nonphagocytic cellsMaria Lara-Tejero
Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, CT 06536, USA
Infect Immun 77:2635-42. 2009..We conclude that the coordinated deployment and interaction between the protein translocases mediate the SPI-1 TTSS-dependent intimate association of S. Typhimurium with host cells...
- Regulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptidesMartin W Bader
Departments of Microbiology and Laboratory Medicine, University of Washington Medical School, Seattle, WA 98195, USA
Mol Microbiol 50:219-30. 2003..Because bacterial resistance to oxidative stress and CAMP are induced during infection of animals, CAMP may be an important signal recognized by bacteria on colonization of animal tissues...
- Complex metabolic phenotypes caused by a mutation in yjgF, encoding a member of the highly conserved YER057c/YjgF family of proteinsJ L Enos-Berlage
Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Bacteriol 180:6519-28. 1998..Results also suggest a connection between function of the isoleucine biosynthetic pathway and the requirement for the pentose phosphate pathway in thiamine synthesis...
- The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1D Hersh
Skirball Institute, Department of Microbiology and Kaplan Cancer Center, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Proc Natl Acad Sci U S A 96:2396-401. 1999..Taken together, the data demonstrate that SipB functions as an analog of the Shigella invasin IpaB...
- Maintenance of nitric oxide and redox homeostasis by the salmonella flavohemoglobin hmpIel Soo Bang
Departments of Microbiology and Laboratory Medicine, University of Washington School of Medicine, Seattle, 98195, USA
J Biol Chem 281:28039-47. 2006..The Hmp flavohemoglobin plays a central role in the response of Salmonella to nitrosative stress but requires precise regulation to avoid the exacerbation of oxidative stress that can result if electrons are shuttled to extraneous iron...
- PduL is an evolutionarily distinct phosphotransacylase involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2Yu Liu
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
J Bacteriol 189:1589-96. 2007..97 mM, respectively. Sequence analyses showed that PduL is unrelated in amino acid sequence to known PTAC enzymes and that PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya...
- YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate mediumMelissa R Christopherson
Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, USA
J Bacteriol 190:3057-62. 2008..From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants...
- The PduX enzyme of Salmonella enterica is an L-threonine kinase used for coenzyme B12 synthesisChenguang Fan
Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 283:11322-9. 2008..To our knowledge, PduX is the first enzyme shown to phosphorylate free L-threonine and the first L-threonine kinase shown to function in coenzyme B(12) synthesis...
- Promoter strength properties of the complete sigma E regulon of Escherichia coli and Salmonella entericaVivek K Mutalik
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94158 2517, USA
J Bacteriol 191:7279-87. 2009....
- The phage shock protein PspA facilitates divalent metal transport and is required for virulence of Salmonella enterica sv. TyphimuriumJoyce E Karlinsey
Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA
Mol Microbiol 78:669-85. 2010..We propose that PspA promotes Salmonella virulence by maintaining proton motive force, which is required for the function of multiple transporters mediating bacterial divalent metal acquisition during infection...
- A mutation in the poxA gene of Salmonella enterica serovar Typhimurium alters protein production, elevates susceptibility to environmental challenges, and decreases swine colonizationShawn M D Bearson
Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, 1920 Dayton Ave, Room 1403, Ames, IA 50010, USA
Foodborne Pathog Dis 8:725-32. 2011....
- Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulenceWen Tssann Liu
Institute for Cell and Molecular Biosciences and School of Biomedical Sciences, The Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, UK
Microb Pathog 42:2-10. 2007..An in silico screen of the Salmonella genome sequence revealed that Salmonella typhimurium LT2 possesses a homologue belonging to the universal stress protein A (UspA) family...
- A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulenceS I Miller
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
Proc Natl Acad Sci U S A 86:5054-8. 1989..When used as live vaccines in mice, strains with phoP or phoQ mutations afford partial protection to subsequent challenge by wild-type S. typhimurium...
- Molecular characterization of the oafA locus responsible for acetylation of Salmonella typhimurium O-antigen: oafA is a member of a family of integral membrane trans-acylasesJ M Slauch
Department of Microbiology, University of Illinois, Urbana 61801, USA
J Bacteriol 178:5904-9. 1996..In many of these instances, acylation defines serotype or host range and thus has a profound effect on microbe-host interaction...
- Identification and characterization of mutants with increased expression of hilA, the invasion gene transcriptional activator of Salmonella typhimuriumT F Fahlen
Department of Microbiology, University of Iowa School of Medicine, 3 330 Bowen Science Bldg, 51 Newton Rd, Iowa City, IA 52242 1109, USA
FEMS Immunol Med Microbiol 28:25-35. 2000..These data implicate new regions of SPI-1 as being involved in the regulation of invasion by S. typhimurium and identify new invasion gene regulators located outside of SPI-1...
- A gyrase mutant with low activity disrupts supercoiling at the replication terminusZhenhua Pang
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 35294, USA
J Bacteriol 187:7773-83. 2005..With more than twice the normal mean number of supercoil domains, this gyrase hypomorph is prone to fork collapse and topological chaos near the terminus of DNA replication...
- Bacterial sensing of and resistance to gold saltsSusana K Checa
Instituto de Biologia Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Departamento de Microbiologia, Universidad Nacional de Rosario, Rosario, Argentina
Mol Microbiol 63:1307-18. 2007..We propose that GolS and its homologues evolved to cope with toxic concentration of Au ion, allowing microorganisms to withstand contaminated environments...
- T-POP array identifies EcnR and PefI-SrgD as novel regulators of flagellar gene expressionChristopher E Wozniak
Department of Microbiology, University of Washington, Seattle, 98195, USA
J Bacteriol 191:1498-508. 2009..These results identify EcnR and PefI-SrgD as additional components of the complex regulatory network controlling flagellar expression...
- Alternative periplasmic copper-resistance mechanisms in Gram negative bacteriaLucas B Pontel
Instituto de Biologia Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Departamento de Microbiologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina
Mol Microbiol 73:212-25. 2009..coli. We posit that CueP can functionally replace the Cus complex for periplasmic copper resistance, in particular under anaerobic conditions...
- LuxS-based quorum sensing does not affect the ability of Salmonella enterica serovar Typhimurium to express the SPI-1 type 3 secretion system, induce membrane ruffles, or invade epithelial cellsCharlotte A Perrett
Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
J Bacteriol 191:7253-9. 2009..Genetic deletion of luxS did not alter the virulence of S. Typhimurium in the mouse model, and therefore, it appears that luxS does not play a significant role in regulating invasion of Salmonella in vitro or in vivo...
- Characterization of the PduS cobalamin reductase of Salmonella enterica and its role in the Pdu microcompartmentShouqiang Cheng
Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA
J Bacteriol 192:5071-80. 2010..These studies further characterize PduS, an unusual MCP-associated cobalamin reductase, and, in conjunction with prior results, indicate that the Pdu MCP encapsulates a complete cobalamin assimilation system...
- SopB, a protein required for virulence of Salmonella dublin, is an inositol phosphate phosphataseF A Norris
Division of Hematology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO, 63110, USA
Proc Natl Acad Sci U S A 95:14057-9. 1998..dublin SB c/s do not accumulate high levels of inositol 1,4,5,6-tetrakisphosphate that are characteristic of wild-type S. dublin-infected cells. Therefore, SopB mediates virulence by interdicting inositol phosphate signaling pathways...
- A small protein that mediates the activation of a two-component system by another two-component systemL F Kox
Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110 1093, USA
EMBO J 19:1861-72. 2000..A pmrD mutant is sensitive to polymyxin if grown in low magnesium, but resistant if grown in high iron. The PmrD protein controls the activity of the PmrA-PmrB system at a post-transcriptional level...
- Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinantsRobert A Kingsley
Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
Infect Immun 71:629-40. 2003..These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice...
- Lsr-mediated transport and processing of AI-2 in Salmonella typhimuriumMichiko E Taga
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
Mol Microbiol 50:1411-27. 2003..Transport and processing of AI-2 could be required for removing the quorum-sensing signal, conveying the signal to an internal detector and/or scavenging boron...
- SseL is a salmonella-specific translocated effector integrated into the SsrB-controlled salmonella pathogenicity island 2 type III secretion systemBrian K Coombes
Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, Room 4H17, 1200 Main St West, Hamilton, Ontario L8N 3Z5, Canada
Infect Immun 75:574-80. 2007..Animal infection experiments with sseL mutant bacteria indicate that integration of SseL into the SsrB response regulatory system contributes to systemic virulence of this pathogen...
- Role of FimW, FimY, and FimZ in regulating the expression of type i fimbriae in Salmonella enterica serovar TyphimuriumSupreet Saini
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana Champaign, Urbana, IL 61810, USA
J Bacteriol 191:3003-10. 2009..However, we found that the response was continuous and not bistable...
- Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella entericaChenguang Fan
Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 284:20240-8. 2009..The studies reported here are the first to investigate the kinetic and catalytic mechanisms of l-threonine kinase from any organism...
- Salmonella pathogenicity island 2 expression negatively controlled by EIIANtr-SsrB interaction is required for Salmonella virulenceJeongjoon Choi
Department of Food and Animal Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151 921, Korea
Proc Natl Acad Sci U S A 107:20506-11. 2010..These results indicated that normal SPI-2 gene expression maintained by an EIIA(Ntr)-SsrB interaction is another determinant of Salmonella virulence...
- Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar TyphimuriumBryan Troxell
Department of Microbiology, North Carolina State University, Raleigh, NC 27695 7615, USA
J Bacteriol 193:497-505. 2011..Finally, deletion of fur in an hns mutant resulted in Fur-independent activation of hilA. In conclusion, Fur activates hilA by repressing the expression of hns...
- Response regulator ArcA of Salmonella enterica serovar Typhimurium downregulates expression of OmpD, a porin facilitating uptake of hydrogen peroxideIván L Calderón
Laboratorio de Microbiologia Molecular, Facultad de Ciencias Biologicas, Universidad Andres Bello, Republica 217, Santiago, Chile
Res Microbiol 162:214-22. 2011....
- Identification of two targets of the type III protein secretion system encoded by the inv and spa loci of Salmonella typhimurium that have homology to the Shigella IpaD and IpaA proteinsK Kaniga
Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook 11794 5222, USA
J Bacteriol 177:7078-85. 1995..In addition, we have found an open reading frame immediately downstream of SipA that encodes a predicted protein with significant similarity to a family of acyl carrier proteins...
- Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq geneL Brown
Department of Microbiology and Immunology, West Virginia University Health Sciences Center, Morgantown, West Virginia 26506, USA
J Bacteriol 178:3763-70. 1996..typhimurium hfq mutant has a four- to sevenfold reduction in expression of rpoS that is attributable primarily to a defect in translation. These results add a new level of complexity to the regulation of RpoS activity...
- Salmonella SsrB activates a global regulon of horizontally acquired genesM J Worley
Department of Microbiology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201, USA
Mol Microbiol 36:749-61. 2000..The SsrB regulon is modulated by the two-component regulatory systems PhoP/PhoQ and OmpR/EnvZ, and is upregulated in the intracellular microenvironment...
- Hha is a negative modulator of transcription of hilA, the Salmonella enterica serovar Typhimurium invasion gene transcriptional activatorT F Fahlen
Department of Microbiology, University of Iowa School of Medicine, Iowa City 52242, USA
J Bacteriol 183:6620-9. 2001..These data add detail to the developing model of the regulation of Salmonella invasion genes...
- The Salmonella effector protein SopB protects epithelial cells from apoptosis by sustained activation of AktLeigh A Knodler
Laboratory of Intracellular Parasites, NIAID, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
J Biol Chem 280:9058-64. 2005..These results provide new insights into the mechanisms of apoptosis and highlight how bacterial effectors can intercept signaling pathways to manipulate host responses...
- Thioredoxin 1 promotes intracellular replication and virulence of Salmonella enterica serovar TyphimuriumEva Bjur
Microbiology and Tumor Biology Center, Karolinska Institutet, Nobels vag 16, 177 71 Stockholm, Sweden
Infect Immun 74:5140-51. 2006..These results clearly imply that in S. enterica serovar Typhimurium, the redox-active protein thioredoxin 1 promotes virulence, whereas in vitro tolerance to oxidative stress depends on production of glutathione...
- Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyaseYongqun Zhu
National Institute of Biological Sciences, Beijing, 102206, China
Mol Cell 28:899-913. 2007..The mechanism described here provides a structural understanding of MAPK inactivation by a family of pathogenic effectors conserved in plant and animal systems and may also open a new route for biological catalysis...
- The Salmonella SPI-2 effector SseJ exhibits eukaryotic activator-dependent phospholipase A and glycerophospholipid : cholesterol acyltransferase activityNadine S Lossi
Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK
Microbiology 154:2680-8. 2008..Analysis of the activating factor(s) shows that it is soluble and heat- and protease-sensitive. We conclude that PLA and GCAT activities of SseJ are potentiated by proteinaceous eukaryotic factor(s)...
- Novel role of the nitrite transporter NirC in Salmonella pathogenesis: SPI2-dependent suppression of inducible nitric oxide synthase in activated macrophagesPriyanka Das
Center for Infectious Disease Research and Biosafety Laboratories, Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
Microbiology 155:2476-89. 2009..Taken together, these results reveal that intracellular Salmonella evade killing in activated macrophages by downregulating IFN-gamma-induced NO production, and they highlight the critical role of nirC as a virulence gene...
- Salmonella modulates vesicular traffic by altering phosphoinositide metabolismLorraine D Hernandez
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA
Science 304:1805-7. 2004..Absence of SopB caused a significant defect in the maturation of the Salmonella-containing vacuole and impaired bacterial intracellular growth...
- The Salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagC and ailE J Heffernan
Department of Medicine, University of California, San Diego 92103
J Bacteriol 174:84-91. 1992..Rck, most closely related to PagC, represents the third outer membrane protein in this five-member family with a distinct virulence-associated phenotype...
- Transcriptional autoregulation of the Salmonella typhimurium phoPQ operonF C Soncini
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Bacteriol 177:4364-71. 1995..A return to basal levels could be mediated by a posttranscriptional mechanism by which translation of the mRNA produced from phoPp1 is inhibited...
- Identification and sequence analysis of lpfABCDE, a putative fimbrial operon of Salmonella typhimuriumA J Bäumler
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201 3098, USA
J Bacteriol 177:2087-97. 1995..Genetically, the lpf operon was found to be most closely related to the fim operon of S. typhimurium, both in gene order and in conservation of the deduced amino acid sequences...
- Nucleotide sequence of a 13.9 kb segment of the 90 kb virulence plasmid of Salmonella typhimurium: the presence of fimbrial biosynthetic genesM J Friedrich
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908
Mol Microbiol 8:543-58. 1993..Two of the other proteins were homologous to transcription regulatory proteins and a third was the rck gene, which encodes an outer membrane protein that confers complement resistance to serum-sensitive hosts...
- A PhoP-repressed gene promotes Salmonella typhimurium invasion of epithelial cellsI Behlau
Infectious Disease Unit, Massachusetts General Hospital, Boston
J Bacteriol 175:4475-84. 1993..Therefore, two virulence properties of Salmonella species, induction of endocytosis by epithelial cells and survival within macrophages, are oppositely modulated by the PhoP/PhoQ virulence regulators...
- Mg2+ as an extracellular signal: environmental regulation of Salmonella virulenceE García Véscovi
Washington University School of Medicine, Department of Molecular Microbiology, St Louis, Missouri 63110, USA
Cell 84:165-74. 1996..Mg2+ changed the conformation of the periplasmic domain of PhoQ, identifying this protein as a Mg2+ sensor. A mutation in the sensing domain of PhoQ altered the set point for Mg2+ and rendered Salmonella avirulent...
- Identification of a Salmonella virulence gene required for formation of filamentous structures containing lysosomal membrane glycoproteins within epithelial cellsM A Stein
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada
Mol Microbiol 20:151-64. 1996..Collectively, these results suggest that sifA arose by horizontal gene transfer into Salmonella and its product is involved in a virulence-associated intracellular phenotype related to Salmonella-induced filament formation...
- The invasion-associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cellC M Collazo
Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794 5222, USA
Mol Microbiol 24:747-56. 1997..These results indicate that protein translocation into host cells may be a universal function of all type III secretion systems...
- A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteriaW D Hardt
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794 5222, USA
Proc Natl Acad Sci U S A 94:9887-92. 1997..They may therefore constitute a novel family of effector proteins with related functions in the cross-talk of these pathogens with their hosts...
- SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophagesN Buchmeier
Department of Pathology, University of California, San Diego, La Jolla 92093 0640, USA
Infect Immun 65:3725-30. 1997..These results suggest that the SlyA regulon is activated during infection of the host and is required for resistance to toxic oxidative products of the reticuloendothelial system...
- The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survivalA B Blanc-Potard
Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, Campus Box 8230, St Louis, MO 63110, USA
EMBO J 16:5376-85. 1997....
- A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosaE E Galyov
Institute for Animal Health, Nr Newbury, Berkshire, UK
Mol Microbiol 25:903-12. 1997....
- Lipid A mutant Salmonella with suppressed virulence and TNFalpha induction retain tumor-targeting in vivoK B Low
Department of Therapeutic Radiology, Yale University, School of Medicine, New Haven, CT 05620, USA
Nat Biotechnol 17:37-41. 1999..Lipid modification of tumor-specific bacterial vectors provides a means for reducing septic shock and further suggests that the antitumor activity of these bacteria may be independent of TNFalpha...
- Differential regulation of Salmonella typhimurium type III secreted proteins by pathogenicity island 1 (SPI-1)-encoded transcriptional activators InvF and hilAK Eichelberg
Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, Connecticut 06536 0812, USA
Infect Immun 67:4099-105. 1999..Our results therefore indicate that InvF and HilA differentially control the expression of components and substrates of the invasion-associated type III secretion system...
- Characterization of two novel regulatory genes affecting Salmonella invasion gene expressionC Altier
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
Mol Microbiol 35:635-46. 2000....
- The putative invasion protein chaperone SicA acts together with InvF to activate the expression of Salmonella typhimurium virulence genesK H Darwin
Division of Infectious Disease, Departments of Pediatrics and Molecular Microbiology, Washington University School of Medicine and St Louis Children s Hospital, St Louis, MO 63110, USA
Mol Microbiol 35:949-60. 2000..This is the first demonstration of a positive regulatory role for a putative type III secretion system chaperone in the expression of virulence genes...
- Identification of SopE2, a Salmonella secreted protein which is highly homologous to SopE and involved in bacterial invasion of epithelial cellsC S Bakshi
Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom
J Bacteriol 182:2341-4. 2000..SopE2 is highly homologous to SopE, a protein encoded by a gene within a temperate bacteriophage and present in only some pathogenic strains...
- Transcriptional organization and function of invasion genes within Salmonella enterica serovar Typhimurium pathogenicity island 1, including the prgH, prgI, prgJ, prgK, orgA, orgB, and orgC genesJ R Klein
Department of Microbiology, University of Iowa School of Medicine, Iowa City, Iowa 52242 1109, USA
Infect Immun 68:3368-76. 2000..To more fully characterize this invasion operon, we demonstrate that the prgH, prgI, prgJ, prgK, orgA, and orgB genes are each required for invasion and secretion, while orgC is not essential for the invasive phenotype...
- A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimuriumE A Miao
Department of Microbiology, University of Washington, HSB K 140, Box 357710, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 97:7539-44. 2000..Three STE genes (sspH1, sspH2, and sseI) are located within temperate bacteriophages, suggesting a common mechanism for the dissemination of more recently evolved STE...
- A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalizationD Zhou
Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA
Mol Microbiol 39:248-59. 2001..The ability of Salmonella to stimulate Rho GTPases signalling and inositol phosphate metabolism through alternative mechanisms is an example of the remarkable ability of this bacterial pathogen to manipulate host cellular functions...
- Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimuriumK H Darwin
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
EMBO J 20:1850-62. 2001..The regulation of effector genes described here for InvF and SicA may represent a new paradigm for regulation of virulence in a wide variety of pathogens...
- Control of actin turnover by a salmonella invasion proteinEmma J McGhie
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
Mol Cell 13:497-510. 2004..These data suggest that SipA focuses host cytoskeletal reorganization by locally inhibiting both ADF/cofilin- and gelsolin-directed actin disassembly, while simultaneously stimulating pathogen-induced actin polymerization...
- Identification of host-specific colonization factors of Salmonella enterica serovar TyphimuriumEirwen Morgan
Division of Microbiology, Institute for Animal Health, Compton, Nr Newbury, RG20 7NN, UK
Mol Microbiol 54:994-1010. 2004..We conclude that S. Typhimurium uses different strategies to colonize calves and chicks. This has major implications for vaccine design...
- The related effector proteins SopD and SopD2 from Salmonella enterica serovar Typhimurium contribute to virulence during systemic infection of miceXiuju Jiang
Infection, Immunity, Injury and Repair Program, Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada
Mol Microbiol 54:1186-98. 2004..Our results indicate that both SopD and SopD2 contribute to virulence in mice and suggest a functional relationship between these two proteins during systemic infection of the host...
- Dissecting the PhoP regulatory network of Escherichia coli and Salmonella entericaIgor Zwir
Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, Campus Box 8230, 660 South Euclid Avenue, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 102:2862-7. 2005....
- The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptidesMarie Laure Crouch
Department of Laboratory Medicine, Universit of Washington School of Medicine, Seattle, WA 98195, USA
Mol Microbiol 56:789-99. 2005..In contrast to P2, Crp4 does not inhibit bacterial respiration at lethal concentrations. However, both peptides induce expression of rpoE, suggesting that they trigger a common mechanism for sensing extracytoplasmic stress...
- The intracellular fate of Salmonella depends on the recruitment of kinesinEmmanuel Boucrot
Centre d immunologie de Marseille Luminy, CNRS Inserm Université de la Méditerranée, Parc Scientifique de Luminy, Case 906 13288 Marseille Cedex 9, France
Science 308:1174-8. 2005..Among these effectors, SifA targeted SKIP, a host protein that down-regulated the recruitment of kinesin on the bacterial vacuole and, in turn, controlled vacuolar membrane dynamics...
- The Salmonella effector PipB2 affects late endosome/lysosome distribution to mediate Sif extensionLeigh A Knodler
Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
Mol Biol Cell 16:4108-23. 2005..Therefore, it seems that a divergence in the biological functions of these two effectors can be accounted for by sequence divergence in their C termini...
- Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1Ying Zhang
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 280:38682-8. 2005..Our data suggest that efficient bacterial escape into the cytosol of epithelial cells requires HsRMA1-mediated SopA ubiquitination and contributes to Salmonella-induced enteropathogenicity...
- A common structural motif in the binding of virulence factors to bacterial secretion chaperonesMirjana Lilic
Laboratory of Structural Microbiology, The Rockefeller University, New York, New York 10021, USA
Mol Cell 21:653-64. 2006..Disruption of this structural motif leads to a destabilization of several chaperone-substrate complexes from different species, as well as an impairment of secretion in Salmonella...
- Salmonella enterica serovar Typhimurium effectors SopB, SopE, SopE2 and SipA disrupt tight junction structure and functionErin C Boyle
Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
Cell Microbiol 8:1946-57. 2006..These data suggest that SPI-1-secreted effectors utilize their ability to stimulate Rho family GTPases to disrupt tight junction structure and function...
- Salmonella effector AvrA regulation of colonic epithelial cell inflammation by deubiquitinationZhongde Ye
Department of Pathology, The University of Chicago, Chicago, Illinois, USA
Am J Pathol 171:882-92. 2007..Increased beta-catenin further negatively regulates the NF-kappaB pathway. Our findings suggest an important role for AvrA in regulating host inflammatory responses through NF-kappaB and beta-catenin pathways...
- Selective inhibition of type III secretion activated signaling by the Salmonella effector AvrAFangyong Du
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut, United States of America
PLoS Pathog 5:e1000595. 2009..This interplay between effector proteins delivered by the same TTSS highlights the remarkable complexity of these systems...
- Identification of ancillary fim genes affecting fimA expression in Salmonella typhimuriumD L Swenson
Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242
J Bacteriol 174:7697-704. 1992..In addition, phenotypic expression of type 1 fimbriae by agar-grown cultures is observed only in those transformants bearing plasmids which show increased beta-galactosidase and fimA mRNA levels...
- Implication of quorum sensing in Salmonella enterica serovar typhimurium virulence: the luxS gene is necessary for expression of genes in pathogenicity island 1Jeongjoon Choi
Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Seoul National University, Seoul 151 921, Republic of Korea
Infect Immun 75:4885-90. 2007..Thus, the reduced expression of invF and its regulated genes in Salmonella cells lacking quorum sensing resulted in the attenuation of virulence phenotypes both in vitro and in vivo...
- Missense substitutions lethal to essential functions of EF-TuF Abdulkarim
Department of Molecular Biology, Uppsala University, Sweden
Biochimie 73:1457-64. 1991..The lethal mutations occur in all three structural domains of EF-Tu, but most are in domain II. We aim to use these mutants to define functional domains on EF-Tu...
- The mgtB Mg2+ transport locus of Salmonella typhimurium encodes a P-type ATPaseM D Snavely
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106
J Biol Chem 266:815-23. 1991..Unlike the Ca2(+)-ATPases, however, which mediate efflux of cation from the cytosol, MgtB mediates influx of cation into the cytosol...
- Salmonella typhimurium phoP virulence gene is a transcriptional regulatorE A Groisman
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037
Proc Natl Acad Sci U S A 86:7077-81. 1989..The psiD locus, which is regulated by phosphate availability, was found to be under the control of the phoP gene product. Sequences homologous to phoP were found in several Gram-negative species and in the yeast Saccharomyces cerevisiae...
- Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cellsK Kaniga
Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook 11794 5222, USA
J Bacteriol 177:3965-71. 1995..Nonpolar mutations in sicA, sipB, and sipC rendered S. typhimurium unable to enter cultured epithelial cells, indicating that these genes are required for bacterial internalization...
- The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteinsK Kaniga
Department of Microbiology, School of Medicine, State University of New York at Stony Brook 11794 5222
Mol Microbiol 13:555-68. 1994..This homology suggests that InvG may be necessary for the export of invasion-related determinants or involved in the assembly of a supramolecular structure that promotes entry...
- Molecular characterization of the Salmonella typhimurium flhB operon and its protein productsT Minamino
Faculty of Applied Biological Science, Hiroshima University, Japan
J Bacteriol 176:7630-7. 1994..The results obtained indicated that both proteins are localized in the cytoplasmic membrane...
- Molecular and functional characterization of the Salmonella typhimurium invasion genes invB and invC: homology of InvC to the F0F1 ATPase family of proteinsK Eichelberg
Department of Microbiology, School of Medicine, SUNY Stony Brook 11794 5222
J Bacteriol 176:4501-10. 1994..The role of InvB in this process remains uncertain...
- A cytolysin encoded by Salmonella is required for survival within macrophagesS J Libby
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037
Proc Natl Acad Sci U S A 91:489-93. 1994..The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages...
- Cognate gene clusters govern invasion of host epithelial cells by Salmonella typhimurium and Shigella flexneriE A Groisman
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110
EMBO J 12:3779-87. 1993....
- Salmonella typhimurium responses to a bactericidal protein from human neutrophilsS Y Qi
Department of Biochemistry, University of Southampton, UK
Mol Microbiol 17:523-31. 1995..We therefore conclude that BPI and polymyxin B affect different global regulatory networks in S. typhimurium even though they bind with high affinity to the same cell-surface component...
- Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouseR M Tsolis
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA
Infect Immun 64:4549-56. 1996..The tonB feoB double mutant, given intraperitoneally, was able to infect the liver and spleen at wild-type doses, indicating that additional iron acquisition systems are used during growth at systemic sites of infection...
- PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistanceJ S Gunn
University of Washington, Seattle 98195, USA
J Bacteriol 178:6857-64. 1996..These data suggest that resistance to the polymyxin-CAP family is controlled by a cascade of regulatory protein expression that activates transcription upon environmental sensing...
- Molecular genetic relationships of the salmonellaeE F Boyd
Institute of Molecular Evolutionary Genetics, Mueller Laboratory, Pennsylvania State University, University Park 16802, USA
Appl Environ Microbiol 62:804-8. 1996..enterica. The Salmonella Reference Collection C, composed of the 16 strains used in DNA sequence studies, has been established for research on variation in natural populations...
- Functional analysis of ssaJ and the ssaK/U operon, 13 genes encoding components of the type III secretion apparatus of Salmonella Pathogenicity Island 2M Hensel
Lehrstuhl für Bakteriologie, Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Munchen, Germany
Mol Microbiol 24:155-67. 1997..typhimurium, and is important for epithelial cell invasion. Therefore, mutations in SPI2 can affect the SPI1 secretion system, which raises the possibility of an interaction between the two type III secretion systems...
- A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophageW D Hardt
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794 5222, USA
Proc Natl Acad Sci U S A 95:2574-9. 1998....
- PmrA-PmrB-regulated genes necessary for 4-aminoarabinose lipid A modification and polymyxin resistanceJ S Gunn
University of Washington, Department of Medicine, Seattle 98195, USA
Mol Microbiol 27:1171-82. 1998....
- Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophagesM Hensel
Lehrstuhl für Bakteriologie, Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Munich, Germany
Mol Microbiol 30:163-74. 1998..These data suggest that, in vivo, one of the functions of the SPI-2 secretion system is to enable intracellular bacterial proliferation...
- Triple Resonance Cryoprobe System for a 600 MHz NMRDorothee Kern; Fiscal Year: 2004..The technical skills are available to maintain and operate the instrument at the NMR facility at Brandeis. ..
- NMR STUDIES OF BACTERIAL SIGNAL TRANSDUCTIONDorothee Kern; Fiscal Year: 2004..Methods for segmental isotopic labeling using the splicing enzymes inteins will be combined with recently developed NMR techniques such as TROSY and dipolar couplings in liquid crystalline medium. ..
- Salmonella Invasion Gene RegulationBradley Jones; Fiscal Year: 2004..abstract_text> ..
- Enzyme Dynamics During CatalysisDorothee Kern; Fiscal Year: 2006..The novel approach outlined in this proposal will lead to a deeper and more general understanding of protein dynamics, which should advance (enhance) the success of rational, structure based approaches for drug design. ..
- Interaction of Yersinia with dendritic cellsIgor Brodsky; Fiscal Year: 2007..This knowledge will be important in the development of future strategies against human pathogens. ..
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