Trypanosoma cruzi strain CL Brener

Summary

Alias:

Top Publications

  1. Droll D, Archer S, Fenn K, Delhi P, Matthews K, Clayton C. The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation. FEBS Lett. 2010;584:1156-62 pubmed publisher
    ..We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing. ..
  2. Gluenz E, Taylor M, Kelly J. The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. Int J Parasitol. 2007;37:617-25 pubmed
    ..To investigate the function of Met-III, we used gene deletion. This showed that Met-III is not required for the development of metacyclic trypomastigotes and that null mutants can complete the life-cycle in vitro. ..
  3. Picchi G, Ferreira A, Souza F, Lourenço E, Arauco P, Lorusso A, et al. Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1. Exp Parasitol. 2011;127:147-52 pubmed publisher
    ..We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope. ..
  4. Campos P, Silva V, Furtado C, Machado Silva A, DaRocha W, Peloso E, et al. Trypanosoma cruzi MSH2: Functional analyses on different parasite strains provide evidences for a role on the oxidative stress response. Mol Biochem Parasitol. 2011;176:8-16 pubmed publisher
    ..More importantly, they also indicate that, in addition to its role in MMR, TcMSH2 acts in the parasite response to oxidative stress through a novel mitochondrial function that may be conserved in T. brucei. ..
  5. Paes L, Su rez Mantilla B, Zimbres F, Pral E, Diogo de Melo P, Tahara E, et al. Proline dehydrogenase regulates redox state and respiratory metabolism in Trypanosoma cruzi. PLoS ONE. 2013;8:e69419 pubmed publisher
    ..cruzi life cycle...
  6. Marquez V, Arias D, Piattoni C, Robello C, Iglesias A, Guerrero S. Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi. Antioxid Redox Signal. 2010;12:787-92 pubmed publisher
    ..Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins...
  7. Lima L, Ortiz P, da Silva F, Alves J, Serrano M, Cortez A, et al. Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species. PLoS ONE. 2012;7:e38385 pubmed publisher
    ..Our findings corroborate cruzipain as valuable target for drugs, vaccine, diagnostic and genotyping approaches...
  8. Cribb P, Perozzi M, Villanova G, Trochine A, Serra E. Characterization of TcHMGB, a high mobility group B family member protein from Trypanosoma cruzi. Int J Parasitol. 2011;41:1149-56 pubmed publisher
    ..Protein levels, however, seem to vary throughout the life cycle, which may be related to previously described changes in heterochromatin distribution and transcription rates...
  9. Miranda M, Bouvier L, Canepa G, Pereira C. Subcellular localization of Trypanosoma cruzi arginine kinase. Parasitology. 2009;136:1201-7 pubmed publisher
    ..The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites...
  10. Rodrigues D, Silva R, Rondinelli E, Urm nyi T. Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock. Exp Parasitol. 2010;126:245-53 pubmed publisher
    ..We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi...

Detail Information

Publications49

  1. Droll D, Archer S, Fenn K, Delhi P, Matthews K, Clayton C. The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation. FEBS Lett. 2010;584:1156-62 pubmed publisher
    ..We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing. ..
  2. Gluenz E, Taylor M, Kelly J. The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. Int J Parasitol. 2007;37:617-25 pubmed
    ..To investigate the function of Met-III, we used gene deletion. This showed that Met-III is not required for the development of metacyclic trypomastigotes and that null mutants can complete the life-cycle in vitro. ..
  3. Picchi G, Ferreira A, Souza F, Lourenço E, Arauco P, Lorusso A, et al. Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1. Exp Parasitol. 2011;127:147-52 pubmed publisher
    ..We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope. ..
  4. Campos P, Silva V, Furtado C, Machado Silva A, DaRocha W, Peloso E, et al. Trypanosoma cruzi MSH2: Functional analyses on different parasite strains provide evidences for a role on the oxidative stress response. Mol Biochem Parasitol. 2011;176:8-16 pubmed publisher
    ..More importantly, they also indicate that, in addition to its role in MMR, TcMSH2 acts in the parasite response to oxidative stress through a novel mitochondrial function that may be conserved in T. brucei. ..
  5. Paes L, Su rez Mantilla B, Zimbres F, Pral E, Diogo de Melo P, Tahara E, et al. Proline dehydrogenase regulates redox state and respiratory metabolism in Trypanosoma cruzi. PLoS ONE. 2013;8:e69419 pubmed publisher
    ..cruzi life cycle...
  6. Marquez V, Arias D, Piattoni C, Robello C, Iglesias A, Guerrero S. Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi. Antioxid Redox Signal. 2010;12:787-92 pubmed publisher
    ..Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins...
  7. Lima L, Ortiz P, da Silva F, Alves J, Serrano M, Cortez A, et al. Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species. PLoS ONE. 2012;7:e38385 pubmed publisher
    ..Our findings corroborate cruzipain as valuable target for drugs, vaccine, diagnostic and genotyping approaches...
  8. Cribb P, Perozzi M, Villanova G, Trochine A, Serra E. Characterization of TcHMGB, a high mobility group B family member protein from Trypanosoma cruzi. Int J Parasitol. 2011;41:1149-56 pubmed publisher
    ..Protein levels, however, seem to vary throughout the life cycle, which may be related to previously described changes in heterochromatin distribution and transcription rates...
  9. Miranda M, Bouvier L, Canepa G, Pereira C. Subcellular localization of Trypanosoma cruzi arginine kinase. Parasitology. 2009;136:1201-7 pubmed publisher
    ..The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites...
  10. Rodrigues D, Silva R, Rondinelli E, Urm nyi T. Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock. Exp Parasitol. 2010;126:245-53 pubmed publisher
    ..We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi...
  11. Arias D, Cabeza M, Erben E, Carranza P, Lujan H, T llez I n M, et al. Functional characterization of methionine sulfoxide reductase A from Trypanosoma spp. Free Radic Biol Med. 2011;50:37-46 pubmed publisher
    ..cruzi and Trypanosoma brucei. The results support the occurrence of a metabolic pathway in Trypanosoma spp. involved in the critical function of repairing oxidized macromolecules...
  12. Leroux A, Maugeri D, Opperdoes F, Cazzulo J, Nowicki C. Comparative studies on the biochemical properties of the malic enzymes from Trypanosoma cruzi and Trypanosoma brucei. FEMS Microbiol Lett. 2011;314:25-33 pubmed publisher
    ....
  13. Pierdominici Sottile G, Roitberg A. Proton transfer facilitated by ligand binding. An energetic analysis of the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Biochemistry. 2011;50:836-42 pubmed publisher
    ..This study represents an initial step that can shed light on our understanding of the catalytic mechanism of this reaction...
  14. Vilchez Larrea S, Alonso G, Schlesinger M, Torres H, Flawi M, Fern ndez Villamil S. Poly(ADP-ribose) polymerase plays a differential role in DNA damage-response and cell death pathways in Trypanosoma cruzi. Int J Parasitol. 2011;41:405-16 pubmed publisher
    ..We also believe that we provide the first reported evidence that different proteins could be poly(ADP-ribosyl)ated in response to different stimuli, leading to different cell death pathways...
  15. Ecco G, Vernal J, Razzera G, Tavares C, Serpa V, Arias S, et al. Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database. Gene. 2009;448:1-6 pubmed publisher
    ..cruzi kynureninase. Further experimental assays will be performed to fully understand the importance of this enzyme for T. cruzi metabolism...
  16. Ekanayake D, Sabatini R. Epigenetic regulation of polymerase II transcription initiation in Trypanosoma cruzi: modulation of nucleosome abundance, histone modification, and polymerase occupancy by O-linked thymine DNA glucosylation. Eukaryot Cell. 2011;10:1465-72 pubmed publisher
    ....
  17. Pérez Brandan C, Padilla A, Xu D, Tarleton R, Basombrio M. Knockout of the dhfr-ts gene in Trypanosoma cruzi generates attenuated parasites able to confer protection against a virulent challenge. PLoS Negl Trop Dis. 2011;5:e1418 pubmed publisher
    ..This study indicates that it is possible to generate genetically attenuated T. cruzi parasites able to confer protection against further T. cruzi infections...
  18. Wang H, Kunz S, Chen G, Seebeck T, Wan Y, Robinson H, et al. Biological and structural characterization of Trypanosoma cruzi phosphodiesterase C and Implications for design of parasite selective inhibitors. J Biol Chem. 2012;287:11788-97 pubmed publisher
    ..The structural study also identified a unique parasite pocket that neighbors the active site and may thus be valuable for the design of parasite-specific inhibitors...
  19. Marciano D, Santana M, Nowicki C. Functional characterization of enzymes involved in cysteine biosynthesis and H(2)S production in Trypanosoma cruzi. Mol Biochem Parasitol. 2012;185:114-20 pubmed publisher
    ....
  20. Chung J, Rocha A, Tonelli R, Castilho B, Schenkman S. Eukaryotic initiation factor 5A dephosphorylation is required for translational arrest in stationary phase cells. Biochem J. 2013;451:257-67 pubmed publisher
    ..We conclude that eIF5A phosphorylation and dephosphorylation cycles regulate translation according to the growth conditions...
  21. Bermejo D, Jackson S, Gorosito Serrán M, Acosta Rodriguez E, Amezcua Vesely M, Sather B, et al. Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors RORγt and Ahr that leads to IL-17 production by activated B cells. Nat Immunol. 2013;14:514-22 pubmed publisher
    ..Our combined data suggest that the generation of IL-17(+) B cells may be a previously unappreciated feature of innate immune responses required for pathogen control or IL-17-mediated autoimmunity...
  22. Jha B, Archer S, Clayton C. The trypanosome Pumilio domain protein PUF5. PLoS ONE. 2013;8:e77371 pubmed publisher
    ..Knockdown of PUF5 did not have any effect on the ability of trypanosomes to differentiate from the mammalian to the insect form of the parasite...
  23. Bercovich N, Levin M, Clayton C, Vazquez M. Identification of core components of the exon junction complex in trypanosomes. Mol Biochem Parasitol. 2009;166:190-3 pubmed publisher
    ..We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport...
  24. de Moura M, Schamber Reis B, Passos Silva D, Raj o M, Macedo A, Franco G, et al. Cloning and characterization of DNA polymerase eta from Trypanosoma cruzi: roles for translesion bypass of oxidative damage. Environ Mol Mutagen. 2009;50:375-86 pubmed publisher
    ..However the overexpression of the gene only interferes in response to oxidative lesions, possibly due to the presence of these lesions during the S phase...
  25. Bouvier L, Miranda M, Canepa G, Alves M, Pereira C. An expanded adenylate kinase gene family in the protozoan parasite Trypanosoma cruzi. Biochim Biophys Acta. 2006;1760:913-21 pubmed publisher
    ..coli. This work reports an unusually large number of genes of adenylate kinases and suggests a coordinated regulation of phosphotransferase-mediated ATP regenerating pathways in the unicellular parasite Trypanosoma cruzi...
  26. Raj o M, Passos Silva D, DaRocha W, Franco G, Macedo A, Pena S, et al. DNA polymerase kappa from Trypanosoma cruzi localizes to the mitochondria, bypasses 8-oxoguanine lesions and performs DNA synthesis in a recombination intermediate. Mol Microbiol. 2009;71:185-97 pubmed publisher
    ..cruzi resistance to high doses of gamma irradiation and zeocin and can catalyse DNA synthesis within recombination intermediates...
  27. Baptista C, V ncio R, Abdala S, Carranza J, Westenberger S, Silva M, et al. Differential transcription profiles in Trypanosoma cruzi associated with clinical forms of Chagas disease: Maxicircle NADH dehydrogenase subunit 7 gene truncation in asymptomatic patient isolates. Mol Biochem Parasitol. 2006;150:236-48 pubmed publisher
    ....
  28. da Cunha J, Nakayasu E, de Almeida I, Schenkman S. Post-translational modifications of Trypanosoma cruzi histone H4. Mol Biochem Parasitol. 2006;150:268-77 pubmed publisher
    ..cruzi histone H4 indicate that lysine 4 is acetylated in the majority of histone H4, while other acetylations at the N-terminus portion of histone H4 are less abundant...
  29. Portal P, Fern ndez Villamil S, Alonso G, De Vas M, Flawi M, Torres H, et al. Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance. Mol Biochem Parasitol. 2008;160:42-51 pubmed publisher
    ..Overexpression of TcCPR-B in T. cruzi epimastigotes increased its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole. We suggest a participation of TcCPR-B in the detoxification metabolism of the parasite...
  30. Ochaya S, Respuela P, Simonsson M, Saraswathi A, Branche C, Lee J, et al. Characterization of a Trypanosoma cruzi acetyltransferase: cellular location, activity and structure. Mol Biochem Parasitol. 2007;152:123-31 pubmed publisher
    ..The native protein has a cytosolic cellular location and is present in all three life-cycle stages of the parasite. The recombinant protein was shown to have autoacetylation enzymatic activity...
  31. Fernandez P, Haouz A, Pereira C, Aguilar C, Alzari P. The crystal structure of Trypanosoma cruzi arginine kinase. Proteins. 2007;69:209-12 pubmed publisher
  32. Nardelli S, da Cunha J, Motta M, Schenkman S. Distinct acetylation of Trypanosoma cruzi histone H4 during cell cycle, parasite differentiation, and after DNA damage. Chromosoma. 2009;118:487-99 pubmed publisher
    ..cruzi. While K4 acetylation occurs preferentially in proliferating situations and accumulates in packed chromatin, K10 and K14 acetylations have a particular distribution probably at the boundaries between packed and unpacked chromatin...
  33. Gabelli S, McLellan J, Montalvetti A, Oldfield E, Docampo R, Amzel L. Structure and mechanism of the farnesyl diphosphate synthase from Trypanosoma cruzi: implications for drug design. Proteins. 2006;62:80-8 pubmed publisher
    ....
  34. Rubin de Celis S, Uemura H, Yoshida N, Schenkman S. Expression of trypomastigote trans-sialidase in metacyclic forms of Trypanosoma cruzi increases parasite escape from its parasitophorous vacuole. Cell Microbiol. 2006;8:1888-98 pubmed publisher
    ..These results indicate that the increased expression of TS in cell-derived trypomastigotes is responsible for the earlier exit from the PV to the cytoplasm and their subsequent differentiation into amastigotes...
  35. Uehara L, Moreira O, Oliveira A, Azambuja P, Lima A, Britto C, et al. Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut. PLoS Negl Trop Dis. 2012;6:e1958 pubmed publisher
    ..These data highlight, for the first time, that cruzipain contributes to the interaction of T. cruzi with the insect host...
  36. Figueiredo da Silva A, de Carvalho Vieira L, Krieger M, Goldenberg S, Zanchin N, Guimar es B. Crystal structure of chagasin, the endogenous cysteine-protease inhibitor from Trypanosoma cruzi. J Struct Biol. 2007;157:416-23 pubmed publisher
    ..The knowledge of the chagasin crystal structure may contribute to the elucidation of the molecular mechanism involved in the inhibition of cruzain and other T. cruzi cysteine proteases...
  37. Ram rez G, Valck C, Aguilar L, Kemmerling U, L pez Mu oz R, Cabrera G, et al. Roles of Trypanosoma cruzi calreticulin in parasite-host interactions and in tumor growth. Mol Immunol. 2012;52:133-40 pubmed publisher
    ..A different domain, amino terminal, binds to endothelial cells, thus inhibiting their angiogenic capacity. Since tumor growth depends, to a large extent on angiogenesis, their growth is also inhibited...
  38. Woronowicz A, Amith S, Davis V, Jayanth P, De Vusser K, Laroy W, et al. Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells. Glycobiology. 2007;17:725-34 pubmed publisher
    ..Together, these observations suggest that trypanosome TS mimics neurotrophic factors in cell survival responses against oxidative stress, hypoxia-induced neurite retraction and serum/glucose deprivation...
  39. Ljunggren A, Redzynia I, Alvarez Fernandez M, Abrahamson M, Mort J, Krupa J, et al. Crystal structure of the parasite protease inhibitor chagasin in complex with a host target cysteine protease. J Mol Biol. 2007;371:137-53 pubmed publisher
    ..This information will aid in the development of synthetic inhibitors for use as potential drugs for the treatment of Chagas disease...
  40. Gallo G, Ramos T, Tavares F, Rocha A, Machi E, Schenkman S, et al. Biochemical characterization of a protein tyrosine phosphatase from Trypanosoma cruzi involved in metacyclogenesis and cell invasion. Biochem Biophys Res Commun. 2011;408:427-31 pubmed publisher
    ..In conclusion our results indicate that TcPTP1 is of importance for cellular differentiation and invasivity of this parasite and thus is a valid target for the rational drug design of potential antibiotics directed against T. cruzi...
  41. Piacenza L, Peluffo G, Alvarez M, Kelly J, Wilkinson S, Radi R. Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite. Biochem J. 2008;410:359-68 pubmed publisher
    ..Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance...
  42. Cheleski J, Freitas R, Wiggers H, Rocha J, de Ara jo A, Montanari C. Expression, purification and kinetic characterization of His-tagged glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. Protein Expr Purif. 2011;76:190-6 pubmed publisher
    ..1°C and pH 8.6. Above 37°C, the enzyme activity starts to fall, which may be related to previous reports that the quaternary structure begins a process of disassembly...
  43. Araya J, Cornejo A, Orrego P, Cordero E, Cort z M, Olivares H, et al. Calcineurin B of the human protozoan parasite Trypanosoma cruzi is involved in cell invasion. Microbes Infect. 2008;10:892-900 pubmed publisher
    ..Given that TcCaNB-CL may play a key role in cell invasion and differs considerably in its primary structure from the human CaNB, it might be considered as a potential chemotherapeutic target...
  44. Ratier L, Urrutia M, Paris G, Zarebski L, Frasch A, Goldbaum F. Relevance of the diversity among members of the Trypanosoma cruzi trans-sialidase family analyzed with camelids single-domain antibodies. PLoS ONE. 2008;3:e3524 pubmed publisher
    ..cruzi to evade the host immune system...
  45. Cuellar A, Rojas F, Bola os N, Diez H, Del Carmen Thomas M, Rosas F, et al. Natural CD4(+) T-cell responses against Trypanosoma cruzi KMP-11 protein in chronic chagasic patients. Immunol Cell Biol. 2009;87:149-53 pubmed publisher
    ..This is the first description of T. cruzi KMP-11 protein recognition by CD4(+) T cells in chronic chagasic patients...
  46. Miranda M, Canepa G, Bouvier L, Pereira C. Trypanosoma cruzi nucleoside diphosphate kinase 1 ( TcNDPK1) has a broad nuclease activity. Parasitology. 2008;135:1661-6 pubmed publisher
    ..Furthermore, phenogram analysis showed that TcNDPK1 is close to Drosophila melanogaster and human NDPKs. The unspecific nuclease activity could suggest a participation in cellular processes such as programmed cell death...
  47. Merritt E, Arakaki T, Gillespie J, Larson E, Kelley A, Mueller N, et al. Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs. J Mol Biol. 2010;397:481-94 pubmed publisher
    ..The essentiality of the single HisRS gene in T. brucei is shown by a severe depression of parasite growth rate that results from even partial suppression of expression by RNA interference...
  48. Buchanan K, Ames J, Asfaw S, Wingard J, Olson C, Campana P, et al. A flagellum-specific calcium sensor. J Biol Chem. 2005;280:40104-11 pubmed publisher
    ..These findings may have broader implications for the calcium acyl switch mechanism of protein regulation...
  49. C ceres A, Qui ones W, Gualdr n M, Cordeiro A, Avil n L, Michels P, et al. Molecular and biochemical characterization of novel glucokinases from Trypanosoma cruzi and Leishmania spp. Mol Biochem Parasitol. 2007;156:235-45 pubmed publisher
    ....