Trypanosoma cruzi strain CL Brener

Summary

Alias:

Top Publications

  1. doi Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1
    Gisele F A Picchi
    Fundacao Oswaldo Cruz, Instituto Carlos Chagas, R Professor Algacyr Munhoz Mader, 3775 Cidade Industrial Paraná, 81350 010 Curitiba, PR, Brazil
    Exp Parasitol 127:147-52. 2011
  2. pmc The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation
    Dorothea Droll
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Alliance, Heidelberg, Germany
    FEBS Lett 584:1156-62. 2010
  3. pmc Trypanosoma cruzi MSH2: Functional analyses on different parasite strains provide evidences for a role on the oxidative stress response
    Priscila C Campos
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
    Mol Biochem Parasitol 176:8-16. 2011
  4. pmc The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements
    Eva Gluenz
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Parasitol 37:617-25. 2007
  5. pmc Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP
    Spencer O Moen
    Seattle Structural Genomics Center for Infectious Disease, USA
    Acta Crystallogr F Struct Biol Commun 71:609-14. 2015
  6. doi Roles of Trypanosoma cruzi calreticulin in parasite-host interactions and in tumor growth
    Galia Ramirez
    Department of Preventive Animal Medicine, University of Chile, Santiago, Chile
    Mol Immunol 52:133-40. 2012
  7. doi Exploring the FL-160-CRP gene family through sequence variability of the complement regulatory protein (CRP) expressed by the trypomastigote stage of Trypanosoma cruzi
    Françoise Mathieu-Daudé
    département Sociétés et Santé, UR008 Pathogénie et Epidémiologie des Trypanosomatidés, Institut de Recherche pour le Developpement, 34394 Montpellier, France
    Infect Genet Evol 8:258-66. 2008
  8. pmc Proline dehydrogenase regulates redox state and respiratory metabolism in Trypanosoma cruzi
    Lisvane Silva Paes
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 8:e69419. 2013
  9. doi Expression, functionality, and localization of apurinic/apyrimidinic endonucleases in replicative and non-replicative forms of Trypanosoma cruzi
    S Sepulveda
    Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
    J Cell Biochem 115:397-409. 2014
  10. pmc Evidence of ternary complex formation in Trypanosoma cruzi trans-sialidase catalysis
    Isadora A Oliveira
    From the Laboratório de Glicobiologia Estrutural e Funcional, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ 21941 902, Brazil
    J Biol Chem 289:423-36. 2014

Research Grants

Scientific Experts

  • Masahiro Inoue
  • Ethan Merritt
  • Gisele F A Picchi
  • Cecilia Pérez Brandan
  • Sergio Schenkman
  • Françoise Mathieu Daudé
  • Juan A Venegas
  • Alejandro E Leroux
  • Diego G Arias
  • Daniel Ken Inaoka
  • Bruno Luiz Fonseca Schamber-Reis
  • Carlos Renato Machado
  • Sergio A Guerrero
  • Roberto Docampo
  • Hector N Torres
  • Guillermo D Alonso
  • Mirtha M Flawia
  • Matias G De Vas
  • Nobuko Yoshida
  • Patricio Portal
  • Jorge E Araya
  • Mariana R Miranda
  • Claudio A Pereira
  • Julia Pinheiro Chagas da Cunha
  • Leon A Bouvier
  • Spencer O Moen
  • Zabdi González-Chávez
  • Sayantanee Niyogi
  • Aracelys López-Castilla
  • S Sepulveda
  • Isadora A Oliveira
  • Fernando Yukio Maeda
  • Ligia Cristina Kalb
  • Bhaskar Anand Jha
  • Leticia Pérez-Díaz
  • Lisvane Silva Paes
  • Janete Chung
  • Daniela A Bermejo
  • Antonio A Rocha
  • Huanchen Wang
  • Luciana Lima
  • Lívia Almeida Uehara
  • Galia Ramirez
  • Daniela Marciano
  • Carlos A Labriola
  • Cecilia Ortiz
  • Gloria Gallo
  • Pamela Cribb
  • Gustavo Pierdominici-Sottile
  • María Dolores Piñeyro
  • Dilrukshi Ekanayake
  • Juliana Cheleski
  • Priscila C Campos
  • Mariana Schlesinger
  • Salomé C Vilchez Larrea
  • Alberto A Iglesias
  • Glória Regina Franco
  • Sergio Danilo Junho Pena
  • Andrea Mara Macedo
  • Santuza Maria Ribeiro Teixeira
  • Carlos Robello
  • Dorothea Droll
  • Deivid C Rodrigues
  • Vanina E Marquez
  • Vicente de Paulo Martins
  • Cristina Paveto
  • Christine Clayton
  • Silvia Fernández Villamil
  • Adriana Cuellar
  • Maria Cristina M Motta
  • Bessy Gutiérrez
  • Natalia Bercovich
  • Sheila Cristina Nardelli
  • Yael Garcia-Marchan
  • Gabriela Ecco
  • Michelle Barbi de Moura
  • M A Rajão
  • Jorge Gonzalez
  • Hernan Sagua
  • Françoise Mathieu-Daudé
  • Adeilton Brandao
  • Marco Aurelio Krieger
  • Lucia Piacenza
  • Samuel Goldenberg
  • M R Miranda
  • Laura Ratier
  • Rosane Silva
  • Turan P Urmenyi
  • Edson Rondinelli
  • Gaspar E Canepa

Detail Information

Publications75

  1. doi Trypanosoma cruzi: identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1
    Gisele F A Picchi
    Fundacao Oswaldo Cruz, Instituto Carlos Chagas, R Professor Algacyr Munhoz Mader, 3775 Cidade Industrial Paraná, 81350 010 Curitiba, PR, Brazil
    Exp Parasitol 127:147-52. 2011
    ..We therefore suggest that TcNUP-1 is a structural protein that plays an essential role in nuclear organization by anchoring T. cruzi chromosomes to the nuclear envelope...
  2. pmc The trypanosome Pumilio-domain protein PUF7 associates with a nuclear cyclophilin and is involved in ribosomal RNA maturation
    Dorothea Droll
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Alliance, Heidelberg, Germany
    FEBS Lett 584:1156-62. 2010
    ..We show here that epitope-tagged TbPUF7 is located in the nucleolus, and associated with a nuclear cyclophilin-like protein, TbNCP1. RNAi targeting PUF7 reduced trypanosome growth and inhibited two steps in ribosomal RNA processing...
  3. pmc Trypanosoma cruzi MSH2: Functional analyses on different parasite strains provide evidences for a role on the oxidative stress response
    Priscila C Campos
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
    Mol Biochem Parasitol 176:8-16. 2011
    ..More importantly, they also indicate that, in addition to its role in MMR, TcMSH2 acts in the parasite response to oxidative stress through a novel mitochondrial function that may be conserved in T. brucei...
  4. pmc The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements
    Eva Gluenz
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Parasitol 37:617-25. 2007
    ..To investigate the function of Met-III, we used gene deletion. This showed that Met-III is not required for the development of metacyclic trypomastigotes and that null mutants can complete the life-cycle in vitro...
  5. pmc Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP
    Spencer O Moen
    Seattle Structural Genomics Center for Infectious Disease, USA
    Acta Crystallogr F Struct Biol Commun 71:609-14. 2015
    ..major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target...
  6. doi Roles of Trypanosoma cruzi calreticulin in parasite-host interactions and in tumor growth
    Galia Ramirez
    Department of Preventive Animal Medicine, University of Chile, Santiago, Chile
    Mol Immunol 52:133-40. 2012
    ..A different domain, amino terminal, binds to endothelial cells, thus inhibiting their angiogenic capacity. Since tumor growth depends, to a large extent on angiogenesis, their growth is also inhibited...
  7. doi Exploring the FL-160-CRP gene family through sequence variability of the complement regulatory protein (CRP) expressed by the trypomastigote stage of Trypanosoma cruzi
    Françoise Mathieu-Daudé
    département Sociétés et Santé, UR008 Pathogénie et Epidémiologie des Trypanosomatidés, Institut de Recherche pour le Developpement, 34394 Montpellier, France
    Infect Genet Evol 8:258-66. 2008
    ..This work provides also information for the T. cruzi genome annotation of the trans-sialidase-like putative proteins...
  8. pmc Proline dehydrogenase regulates redox state and respiratory metabolism in Trypanosoma cruzi
    Lisvane Silva Paes
    Departamento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil
    PLoS ONE 8:e69419. 2013
    ..cruzi life cycle. ..
  9. doi Expression, functionality, and localization of apurinic/apyrimidinic endonucleases in replicative and non-replicative forms of Trypanosoma cruzi
    S Sepulveda
    Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
    J Cell Biochem 115:397-409. 2014
    ..Our results confirm that the BER pathway is involved in T. cruzi resistance to DNA oxidative damage and points to the participation of DNA AP endonucleases in parasite survival...
  10. pmc Evidence of ternary complex formation in Trypanosoma cruzi trans-sialidase catalysis
    Isadora A Oliveira
    From the Laboratório de Glicobiologia Estrutural e Funcional, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ 21941 902, Brazil
    J Biol Chem 289:423-36. 2014
    ..In summary, our data put forward a coherent dynamic framework to understand how a glycosidase evolved its highly efficient trans-glycosidase activity. ..
  11. doi NMR structure and dynamics of Q4D059, a kinetoplastid-specific and conserved protein from Trypanosoma cruzi
    Aracelys López-Castilla
    Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, RJ, Brazil
    J Struct Biol 190:11-20. 2015
    ..Q4D059 adopts a α/β fold that is slightly similar to the ATPase sub-domain IIB of the heat-shock protein 70 (HSP70) and to the N-terminal domain of the ribosomal protein L11...
  12. doi Metabolic control analysis of the Trypanosoma cruzi peroxide detoxification pathway identifies tryparedoxin as a suitable drug target
    Zabdi González-Chávez
    Departamento de Bioquimica, Instituto Nacional de Cardiologia Ignacio Chavez, México D F 14080, Mexico
    Biochim Biophys Acta 1850:263-73. 2015
    ..Here, Metabolic Control Analysis (MCA) was applied to quantitatively prioritize drug target(s) within the pathway by identifying its flux-controlling enzymes...
  13. pmc Fibronectin-degrading activity of Trypanosoma cruzi cysteine proteinase plays a role in host cell invasion
    Fernando Yukio Maeda
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    Infect Immun 82:5166-74. 2014
    ..Overall, our results have indicated that the cysteine proteinase of T. cruzi metacyclic forms, through its fibronectin-degrading activity, is implicated in host cell invasion. ..
  14. pmc A novel role of Rab11 in trafficking GPI-anchored trans-sialidase to the plasma membrane of Trypanosoma cruzi
    Sayantanee Niyogi
    a Center for Tropical and Emerging Global Diseases and Department of Cellular Biology University of Georgia Athens, Georgia, USA
    Small Gtpases 6:8-10. 2015
    ..We also found that traffic of other GPI-anchored proteins is also through the CVC but uses a Rab11-independent pathway. These represent unconventional pathways of GPI-anchored protein traffic to the plasma membrane. ..
  15. pmc The trypanosome Pumilio domain protein PUF5
    Bhaskar Anand Jha
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Allianz, Heidelberg, Germany
    PLoS ONE 8:e77371. 2013
    ..Knockdown of PUF5 did not have any effect on the ability of trypanosomes to differentiate from the mammalian to the insect form of the parasite. ..
  16. pmc Clathrin expression in Trypanosoma cruzi
    Ligia Cristina Kalb
    Laboratory of Cell Biology, Carlos Chagas Institute, FIOCRUZ, Rua Professor Algacyr Munhoz Mader 3775, 81350 010 Curitiba, PR, Brazil
    BMC Cell Biol 15:23. 2014
    ..cruzi (epimastigotes, trypomastigotes and amastigotes), using both polyclonal and monoclonal antibodies raised against T. cruzi recombinant proteins...
  17. doi Evidence for a negative feedback control mediated by the 3' untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
    Leticia Pérez-Díaz
    Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Igua 4225, 11400 Montevideo, Uruguay
    Biochem Biophys Res Commun 436:295-9. 2013
    ..The mechanism revealed here adds to our limited but growing number of examples of negative mRNA autoregulation in the control of gene expression. ..
  18. doi The NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol biosynthesis pathway
    Matias G De Vas
    Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
    Int J Parasitol 41:99-108. 2011
    ..In addition, we suggest a different role for TcCPR-A, since its over-expression is lethal, displaying cells with an increased DNA content, aberrant morphology and severe ultrastructural alterations...
  19. pmc Functional cooperation between BiP and calreticulin in the folding maturation of a glycoprotein in Trypanosoma cruzi
    Carlos A Labriola
    Laboratories of Glycobiology, Fundación Instituto Leloir and Instituto de Investigaciones Bioquímicas de Buenos Aires IIBBA CONICET, Buenos Aires, Argentina
    Mol Biochem Parasitol 175:112-7. 2011
    ..The present report provides further evidence on the early evolutionary acquisition of the basic tenets of the N-glycan dependent quality control mechanism of glycoprotein folding...
  20. doi Functional characterization of methionine sulfoxide reductase A from Trypanosoma spp
    Diego G Arias
    Instituto de Agrobiotecnología del Litoral, UNL CONICET, 3000 Santa Fe, Argentina
    Free Radic Biol Med 50:37-46. 2011
    ..cruzi and Trypanosoma brucei. The results support the occurrence of a metabolic pathway in Trypanosoma spp. involved in the critical function of repairing oxidized macromolecules...
  21. doi Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock
    Deivid C Rodrigues
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Exp Parasitol 126:245-53. 2010
    ..We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi...
  22. doi Comparative studies on the biochemical properties of the malic enzymes from Trypanosoma cruzi and Trypanosoma brucei
    Alejandro E Leroux
    Instituto de Química y Fisicoquímica Biológica IQUIFIB CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina
    FEMS Microbiol Lett 314:25-33. 2011
    ....
  23. doi Expression, purification and kinetic characterization of His-tagged glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi
    Juliana Cheleski
    Grupo de Estudos em Quimica Medicinal de Produtos Naturais NEQUIMED PN, Instituto de Quimica de Sao Carlos, Universidade de Sao Paulo, Sao Carlos, SP, Brazil
    Protein Expr Purif 76:190-6. 2011
    ..1°C and pH 8.6. Above 37°C, the enzyme activity starts to fall, which may be related to previous reports that the quaternary structure begins a process of disassembly...
  24. pmc Proton transfer facilitated by ligand binding. An energetic analysis of the catalytic mechanism of Trypanosoma cruzi trans-sialidase
    Gustavo Pierdominici-Sottile
    Department of Chemistry and Quantum Theory Project, University of Florida, Gainesville, Florida 32611 8435, United States
    Biochemistry 50:836-42. 2011
    ..This study represents an initial step that can shed light on our understanding of the catalytic mechanism of this reaction...
  25. doi Poly(ADP-ribose) polymerase plays a differential role in DNA damage-response and cell death pathways in Trypanosoma cruzi
    Salomé C Vilchez Larrea
    Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Vuelta de Obligado 2490, Ciudad Autonoma de Buenos Aires, 1428 Buenos Aires, Argentina
    Int J Parasitol 41:405-16. 2011
    ..We also believe that we provide the first reported evidence that different proteins could be poly(ADP-ribosyl)ated in response to different stimuli, leading to different cell death pathways...
  26. pmc Phosphorylation-dependent protein interaction with Trypanosoma brucei 14-3-3 proteins that display atypical target recognition
    Masahiro Inoue
    Division of Eukaryotic Microbiology, Department of Infectious Medicine, Kurume University School of Medicine, Kurume, Japan
    PLoS ONE 5:e15566. 2010
    ..In Trypanosoma brucei, 14-3-3I and II play pivotal roles in motility, cytokinesis and the cell cycle. However, none of the T. brucei 14-3-3 binding proteins have previously been documented...
  27. doi Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi
    Alejandro E Leroux
    Instituto de Química y Fisicoquímica Biológica IQUIFIB CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, C1113AAD Buenos Aires, Argentina
    Mol Biochem Parasitol 177:61-4. 2011
    ..This observation fits in well with the expected oxidative burst this pathogen has to face when infecting the mammalian host...
  28. doi Biochemical characterization of a protein tyrosine phosphatase from Trypanosoma cruzi involved in metacyclogenesis and cell invasion
    Gloria Gallo
    Departamento de Biofisica, Universidade Federal de Sao Paulo, Rua Botucatu 862, 04023 062 Sao Paulo, Brazil
    Biochem Biophys Res Commun 408:427-31. 2011
    ..In conclusion our results indicate that TcPTP1 is of importance for cellular differentiation and invasivity of this parasite and thus is a valid target for the rational drug design of potential antibiotics directed against T. cruzi...
  29. doi Molecular characterization and interactome analysis of Trypanosoma cruzi tryparedoxin 1
    María Dolores Piñeyro
    Unidad de Biología Molecular Institut Pasteur de Montevideo, Montevideo, Uruguay
    J Proteomics 74:1683-92. 2011
    ..Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi...
  30. doi Characterization of TcHMGB, a high mobility group B family member protein from Trypanosoma cruzi
    Pamela Cribb
    Instituto de Biologia Molecular y Celular de Rosario IBR CONICET, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario CP2000, Argentina
    Int J Parasitol 41:1149-56. 2011
    ..Protein levels, however, seem to vary throughout the life cycle, which may be related to previously described changes in heterochromatin distribution and transcription rates...
  31. pmc Epigenetic regulation of polymerase II transcription initiation in Trypanosoma cruzi: modulation of nucleosome abundance, histone modification, and polymerase occupancy by O-linked thymine DNA glucosylation
    Dilrukshi Ekanayake
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 7229, USA
    Eukaryot Cell 10:1465-72. 2011
    ....
  32. pmc Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate
    Cecilia Ortiz
    Redox Biology of Trypanosomes Laboratory, Institut Pasteur de Montevideo, Mataojo 2020, 11400 Montevideo, Uruguay
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1457-61. 2011
    ..85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure...
  33. pmc Knockout of the dhfr-ts gene in Trypanosoma cruzi generates attenuated parasites able to confer protection against a virulent challenge
    Cecilia Pérez Brandan
    Instituto de Patología Experimental CONICET, Universidad Nacional de Salta, Salta, Argentina
    PLoS Negl Trop Dis 5:e1418. 2011
    ..In this sense, targeted deletion or disruption of specific genes in the parasite genome may protect against such reversion to virulent genotypes...
  34. pmc Biological and structural characterization of Trypanosoma cruzi phosphodiesterase C and Implications for design of parasite selective inhibitors
    Huanchen Wang
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 287:11788-97. 2012
    ..The structural study also identified a unique parasite pocket that neighbors the active site and may thus be valuable for the design of parasite-specific inhibitors...
  35. doi DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions
    Bruno Luiz Fonseca Schamber-Reis
    Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil
    Mol Biochem Parasitol 183:122-31. 2012
    ..Taken together our data suggest participation of Tcpolβ during kDNA replication and repair of oxidative DNA damage induced by genotoxic stress in this organelle...
  36. pmc Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species
    Luciana Lima
    Departamento de Parasitologia, ICB, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brasil
    PLoS ONE 7:e38385. 2012
    ..Our findings corroborate cruzipain as valuable target for drugs, vaccine, diagnostic and genotyping approaches...
  37. doi Functional characterization of enzymes involved in cysteine biosynthesis and H(2)S production in Trypanosoma cruzi
    Daniela Marciano
    Instituto de Química y Fisicoquímica Biológica IQUIFIB CONICET, Universidad de Buenos Aires, Junin 956, C1113AAD Buenos Aires, Argentina
    Mol Biochem Parasitol 185:114-20. 2012
    ....
  38. pmc Cruzipain promotes Trypanosoma cruzi adhesion to Rhodnius prolixus midgut
    Lívia Almeida Uehara
    Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio de Janeiro, Brazil
    PLoS Negl Trop Dis 6:e1958. 2012
    ..cruzi life cycle and are implicated in parasite-mammalian host relationships. However, little is known about the factors that contribute to the parasite-insect host interaction...
  39. doi Eukaryotic initiation factor 5A dephosphorylation is required for translational arrest in stationary phase cells
    Janete Chung
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Rua Pedro de Toledo 669 L6A, Sao Paulo, S P 04039 032, Brazil
    Biochem J 451:257-67. 2013
    ..We conclude that eIF5A phosphorylation and dephosphorylation cycles regulate translation according to the growth conditions...
  40. pmc Trypanosoma cruzi trans-sialidase initiates a program independent of the transcription factors RORγt and Ahr that leads to IL-17 production by activated B cells
    Daniela A Bermejo
    Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
    Nat Immunol 14:514-22. 2013
    ..Our combined data suggest that the generation of IL-17(+) B cells may be a previously unappreciated feature of innate immune responses required for pathogen control or IL-17-mediated autoimmunity...
  41. ncbi Characterization of a Trypanosoma cruzi acetyltransferase: cellular location, activity and structure
    Stephen Ochaya
    Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius vag 35, Stockholm 17177, Sweden
    Mol Biochem Parasitol 152:123-31. 2007
    ..The native protein has a cytosolic cellular location and is present in all three life-cycle stages of the parasite. The recombinant protein was shown to have autoacetylation enzymatic activity...
  42. doi Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance
    Patricio Portal
    Departamento de Fisiologia, Biologia Molecular y Celular, Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Universidad de Buenos Aires, Buenos Aires, Argentina
    Mol Biochem Parasitol 160:42-51. 2008
    ..Overexpression of TcCPR-B in T. cruzi epimastigotes increased its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole. We suggest a participation of TcCPR-B in the detoxification metabolism of the parasite...
  43. doi Genotyping of Panamanian Trypanosoma cruzi stocks using the calmodulin 3'UTR polymorphisms
    Adeilton Brandao
    Oswaldo Cruz Institute, FIOCRUZ, Rio de Janiero, Brazil
    Parasitol Res 102:523-6. 2008
    ..cruzi isolates from humans and vectors from different endemic areas in Panama. The finding of solely T. cruzi I genotype might explain the low morbidity of Chagas' disease in the region when compared to other countries in Latin America...
  44. pmc Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite
    Lucia Piacenza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 410:359-68. 2008
    ..Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance...
  45. ncbi Molecular and biochemical characterization of novel glucokinases from Trypanosoma cruzi and Leishmania spp
    Ana Judith Cáceres
    Unidad de Bioquimica de Parasitos, Centro de Ingeniería Genética, Facultad de Ciencias, Universidad de Los Andes, Merida 5101, Venezuela
    Mol Biochem Parasitol 156:235-45. 2007
    ....
  46. ncbi The crystal structure of Trypanosoma cruzi glucokinase reveals features determining oligomerization and anomer specificity of hexose-phosphorylating enzymes
    Artur T Cordeiro
    Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology, Avenue Hippocrate 74, B 1200 Brussels, Belgium
    J Mol Biol 372:1215-26. 2007
    ..The preference for distinct anomers suggests that in T. cruzi hexokinase and glucokinase are not directly competing for the same substrate and are probably both present because they exert distinct physiological functions...
  47. ncbi The crystal structure of Trypanosoma cruzi arginine kinase
    Pablo Fernandez
    Unité de Biochimie Structurale, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, France
    Proteins 69:209-12. 2007
  48. ncbi Crystal structure of the parasite protease inhibitor chagasin in complex with a host target cysteine protease
    Anna Ljunggren
    Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, SE 221 85 Lund, Sweden
    J Mol Biol 371:137-53. 2007
    ..This information will aid in the development of synthetic inhibitors for use as potential drugs for the treatment of Chagas disease...
  49. ncbi Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells
    Alicja Woronowicz
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L3N6
    Glycobiology 17:725-34. 2007
    ..Together, these observations suggest that trypanosome TS mimics neurotrophic factors in cell survival responses against oxidative stress, hypoxia-induced neurite retraction and serum/glucose deprivation...
  50. doi Calcineurin B of the human protozoan parasite Trypanosoma cruzi is involved in cell invasion
    Jorge E Araya
    Molecular Parasitology Unit, Department of Medical Technology, University of Antofagasta, P O Box 170, Antofagasta 1240000, Chile
    Microbes Infect 10:892-900. 2008
    ..Given that TcCaNB-CL may play a key role in cell invasion and differs considerably in its primary structure from the human CaNB, it might be considered as a potential chemotherapeutic target...
  51. ncbi Crystal structure of chagasin, the endogenous cysteine-protease inhibitor from Trypanosoma cruzi
    Aline Almeida Figueiredo da Silva
    Centro de Biologia Molecular Estrutural, Laboratorio Nacional de Luz Sincrotron, LNLS, P O Box 6192, CEP 13084 971, Campinas SP, Brazil
    J Struct Biol 157:416-23. 2007
    ..The knowledge of the chagasin crystal structure may contribute to the elucidation of the molecular mechanism involved in the inhibition of cruzain and other T. cruzi cysteine proteases...
  52. ncbi Post-translational modifications of Trypanosoma cruzi histone H4
    Julia Pinheiro Chagas da Cunha
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, R Botucatu 862 8 a, 04023 062 Sao Paulo, Brazil
    Mol Biochem Parasitol 150:268-77. 2006
    ..cruzi histone H4 indicate that lysine 4 is acetylated in the majority of histone H4, while other acetylations at the N-terminus portion of histone H4 are less abundant...
  53. ncbi Differential transcription profiles in Trypanosoma cruzi associated with clinical forms of Chagas disease: Maxicircle NADH dehydrogenase subunit 7 gene truncation in asymptomatic patient isolates
    Cassio S Baptista
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Avenida Professor Lineu Prestes, 748 CEP 05508 000, Sao Paulo, SP, Brazil
    Mol Biochem Parasitol 150:236-48. 2006
    ....
  54. ncbi Expression of trypomastigote trans-sialidase in metacyclic forms of Trypanosoma cruzi increases parasite escape from its parasitophorous vacuole
    Sergio S C Rubin-de-Celis
    Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Sao Paulo, Brazil
    Cell Microbiol 8:1888-98. 2006
    ..These results indicate that the increased expression of TS in cell-derived trypomastigotes is responsible for the earlier exit from the PV to the cytoplasm and their subsequent differentiation into amastigotes...
  55. ncbi An expanded adenylate kinase gene family in the protozoan parasite Trypanosoma cruzi
    Leon A Bouvier
    Departamento de Sustancias Vasoactivas, Instituto de Investigaciones Medicas Alfredo Lanari, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Universidad de Buenos Aires, Combatientes de Malvinas, Buenos Aires, Argentina
    Biochim Biophys Acta 1760:913-21. 2006
    ..coli. This work reports an unusually large number of genes of adenylate kinases and suggests a coordinated regulation of phosphotransferase-mediated ATP regenerating pathways in the unicellular parasite Trypanosoma cruzi...
  56. pmc Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate
    Daniel Ken Inaoka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:875-8. 2005
    ..87, b = 71.89, c = 123.27 A. The presence of two molecules in the asymmetric unit (2 x 34 kDa) gives a crystal volume per protein weight (VM) of 2.2 A3 Da(-1) and a solvent content of 44%...
  57. ncbi Polymorphic and differential expression of the Trypanosoma cruzi alleles containing universal minicircle binding protein
    Elielton R Coelho
    Instituto de Biofisica Carlos Chagas Filho, CCS, Bloco G, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949 900, Brazil
    Biochem Biophys Res Commun 341:382-90. 2006
    ....
  58. ncbi Structure and mechanism of the farnesyl diphosphate synthase from Trypanosoma cruzi: implications for drug design
    Sandra B Gabelli
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Proteins 62:80-8. 2006
    ....
  59. doi Identification of core components of the exon junction complex in trypanosomes
    Natalia Bercovich
    INGEBI CONICET, Vuelta de Obligado 2490, 2P, Buenos Aires, Argentina
    Mol Biochem Parasitol 166:190-3. 2009
    ..We also show that this complex co-purifies in vivo in trypanosomes with a protein containing an NTF2 domain, typically involved in mRNA transport...
  60. ncbi A flagellum-specific calcium sensor
    Kathryn T Buchanan
    Department of Pathology and Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 280:40104-11. 2005
    ..These findings may have broader implications for the calcium acyl switch mechanism of protein regulation...
  61. pmc Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs
    Ethan A Merritt
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Mol Biol 397:481-94. 2010
    ..The essentiality of the single HisRS gene in T. brucei is shown by a severe depression of parasite growth rate that results from even partial suppression of expression by RNA interference...
  62. doi Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi
    Vanina E Marquez
    Instituto de Agrobiotecnología del Litoral, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, Ciudad Universitaria Paraje El Pozo, S3000ZAA Santa Fe, Argentina
    Antioxid Redox Signal 12:787-92. 2010
    ..Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins...
  63. doi Trypanosoma cruzi calmodulin: cloning, expression and characterization
    Yael Garcia-Marchan
    Centro de Biociencias y Medicina Molecular, Instituto de Estudios Avanzados IDEA, Universidad Central de Venezuela, Caracas 1080, Bolivarian Republic of Venezuela
    Exp Parasitol 123:326-33. 2009
    ..cruzi CaM...
  64. doi Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database
    Gabriela Ecco
    Centro de Biologia Molecular Estrutural, Departamento de Bioquimica, Universidade Federal de Santa Catarina, 88040 900 Florianopolis, SC, Brazil
    Gene 448:1-6. 2009
    ..cruzi kynureninase. Further experimental assays will be performed to fully understand the importance of this enzyme for T. cruzi metabolism...
  65. doi Molecular characterization and intracellular distribution of the alpha 5 subunit of Trypanosoma cruzi 20S proteasome
    Bessy Gutiérrez
    Health Sciences Faculty, University of Antofagasta, Antofagasta, Chile
    Parasitol Int 58:367-74. 2009
    ..Proteasome 20S was present in all T. cruzi stages and only slight differences in terms of relative abundance were found. The potential role of the proteasome in kinetoplast remodeling remains to be determined...
  66. doi Subcellular localization of Trypanosoma cruzi arginine kinase
    Mariana R Miranda
    Laboratorio de Biología Molecular de Trypanosoma cruzi LBMTC, Instituto de Investigaciones Médicas IDIM, CONICET and Universidad de Buenos Aires, Buenos Aires, Argentina
    Parasitology 136:1201-7. 2009
    ..The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites...
  67. doi Cloning and characterization of a DNA polymerase beta gene from Trypanosoma cruzi
    Juan A Venegas
    Programa de Biologia Celular y Molecular, ICBM, Universidad de Chile, Casilla 70086, Santiago 7, Chile
    Parasitol Int 58:187-92. 2009
    ....
  68. pmc Developmental expression of a Trypanosoma cruzi phosphoinositide-specific phospholipase C in amastigotes and stimulation of host phosphoinositide hydrolysis
    Vicente de Paulo Martins
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602 2607, USA
    Infect Immun 78:4206-12. 2010
    ..The presence of TcPI-PLC on the outer surface of the plasma membrane of the parasite and the capacity to be secreted and to alter host phospholipids are novel mechanisms of the host-parasite interaction...
  69. doi Distinct acetylation of Trypanosoma cruzi histone H4 during cell cycle, parasite differentiation, and after DNA damage
    Sheila Cristina Nardelli
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Rua Botucatu 862 8A, Sao Paulo, Sao Paulo, 04023 062, Brazil
    Chromosoma 118:487-99. 2009
    ..cruzi. While K4 acetylation occurs preferentially in proliferating situations and accumulates in packed chromatin, K10 and K14 acetylations have a particular distribution probably at the boundaries between packed and unpacked chromatin...
  70. doi Cloning and characterization of DNA polymerase eta from Trypanosoma cruzi: roles for translesion bypass of oxidative damage
    Michelle Barbi de Moura
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, MG, Brazil
    Environ Mol Mutagen 50:375-86. 2009
    ..However the overexpression of the gene only interferes in response to oxidative lesions, possibly due to the presence of these lesions during the S phase...
  71. doi DNA polymerase kappa from Trypanosoma cruzi localizes to the mitochondria, bypasses 8-oxoguanine lesions and performs DNA synthesis in a recombination intermediate
    M A Rajão
    Departamento de Bioquimica e Imunologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Caixa Postal 486, Belo Horizonte 30161 970, MG, Brazil
    Mol Microbiol 71:185-97. 2009
    ..cruzi resistance to high doses of gamma irradiation and zeocin and can catalyse DNA synthesis within recombination intermediates...
  72. doi Trypanosoma cruzi nucleoside diphosphate kinase 1 ( TcNDPK1) has a broad nuclease activity
    M R Miranda
    Laboratorio de Biología Molecular de Trypanosoma cruzi LBMTC, Instituto de Investigaciones Medicas A Lanari, Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, 1427 Buenos Aires, Argentina
    Parasitology 135:1661-6. 2008
    ..Furthermore, phenogram analysis showed that TcNDPK1 is close to Drosophila melanogaster and human NDPKs. The unspecific nuclease activity could suggest a participation in cellular processes such as programmed cell death...
  73. doi Natural CD4(+) T-cell responses against Trypanosoma cruzi KMP-11 protein in chronic chagasic patients
    Adriana Cuellar
    Grupo de Inmunobiología y Biología Celular, Departamento de Microbiologia, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogota, Colombia
    Immunol Cell Biol 87:149-53. 2009
    ..This is the first description of T. cruzi KMP-11 protein recognition by CD4(+) T cells in chronic chagasic patients...
  74. pmc Relevance of the diversity among members of the Trypanosoma cruzi trans-sialidase family analyzed with camelids single-domain antibodies
    Laura Ratier
    Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus IIB INTECH, Universidad Nacional de General San Martin CONICET, Buenos Aires, Argentina
    PLoS ONE 3:e3524. 2008
    ..cruzi to evade the host immune system...
  75. doi Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction
    Daniel Ken Inaoka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Biochemistry 47:10881-91. 2008
    ....

Research Grants2

  1. EXPANDED PROTEIN STRUCTURE REFINEMENT AND VALIDATION
    Ethan Merritt; Fiscal Year: 2005
    ..This, in turn, will make it easier to identify key biological features such as the presence and conformation of bound ligands, and the nature of hinge and inter-domain motions. ..
  2. Software tools for analysis and visulaization of protein structure
    Ethan A Merritt; Fiscal Year: 2010
    ..The algorithms and computation tools developed as part of this work will be distributed freely, and we will provide web-based state of the art analysis of structures submitted by external researchers. ..