Trypanosoma brucei brucei strain 927/4 GUTat10.1

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Top Publications

  1. pmc Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
    T Nicolai Siegel
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10065, USA
    Genes Dev 23:1063-76. 2009
  2. ncbi Flagellar motility is required for the viability of the bloodstream trypanosome
    Richard Broadhead
    Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
    Nature 440:224-7. 2006
  3. pmc Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors
    Daniela Luz Ambrósio
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Eukaryot Cell 8:990-1000. 2009
  4. doi Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei
    Martin Wurst
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, Heidelberg, Germany
    Mol Microbiol 83:1048-63. 2012
  5. ncbi Deletion of the glucosidase II gene in Trypanosoma brucei reveals novel N-glycosylation mechanisms in the biosynthesis of variant surface glycoprotein
    Deuan C Jones
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, The Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 280:35929-42. 2005
  6. ncbi Purification, cloning, and expression of two closely related Trypanosoma brucei nucleic acid binding proteins
    J Zhang
    Department of Microbiology, State University of New York at Buffalo 14214, USA
    Mol Biochem Parasitol 87:145-58. 1997
  7. pmc Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
    D G Cole
    Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Cell Biol 141:993-1008. 1998
  8. doi Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei
    Sabrina Absalon
    Trypanosome Cell Biology Unit, Pasteur Institute and CNRS, 25 rue du Docteur Roux, 75015 Paris, France
    J Cell Sci 121:3704-16. 2008
  9. ncbi Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43
    Xue Hai Liang
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Mol Biochem Parasitol 145:29-39. 2006
  10. pmc Novel roles for the flagellum in cell morphogenesis and cytokinesis of trypanosomes
    Linda Kohl
    INSERM U565 and CNRS UMR8646, Laboratoire de Biophysique, Museum national d histoire naturelle, 43 rue Cuvier, 75231 Paris Cedex 05, France
    EMBO J 22:5336-46. 2003

Scientific Experts

Detail Information

Publications73

  1. pmc Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
    T Nicolai Siegel
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10065, USA
    Genes Dev 23:1063-76. 2009
    ....
  2. ncbi Flagellar motility is required for the viability of the bloodstream trypanosome
    Richard Broadhead
    Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
    Nature 440:224-7. 2006
    ..A postgenomic meta-analysis, comparing the evolutionarily ancient trypanosome with other eukaryotes including humans, identifies numerous trypanosome-specific flagellar proteins, suggesting new avenues for selective intervention...
  3. pmc Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors
    Daniela Luz Ambrósio
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Eukaryot Cell 8:990-1000. 2009
    ..Together, these data strongly indicate that most of the identified proteins are components of the spliceosome...
  4. doi Expression of the RNA recognition motif protein RBP10 promotes a bloodstream-form transcript pattern in Trypanosoma brucei
    Martin Wurst
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, Heidelberg, Germany
    Mol Microbiol 83:1048-63. 2012
    ..Tethering of RBP10 to a reporter mRNA inhibited translation, and halved the abundance of the bound mRNA. We suggest that RBP10 may prevent the expression of regulatory proteins that are specific to the procyclic form...
  5. ncbi Deletion of the glucosidase II gene in Trypanosoma brucei reveals novel N-glycosylation mechanisms in the biosynthesis of variant surface glycoprotein
    Deuan C Jones
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, The Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 280:35929-42. 2005
    ....
  6. ncbi Purification, cloning, and expression of two closely related Trypanosoma brucei nucleic acid binding proteins
    J Zhang
    Department of Microbiology, State University of New York at Buffalo 14214, USA
    Mol Biochem Parasitol 87:145-58. 1997
    ..Both recombinant proteins have been expressed in E. coli and show properties indistinguishable from those observed with native p34/p37...
  7. pmc Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
    D G Cole
    Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Cell Biol 141:993-1008. 1998
    ....
  8. doi Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei
    Sabrina Absalon
    Trypanosome Cell Biology Unit, Pasteur Institute and CNRS, 25 rue du Docteur Roux, 75015 Paris, France
    J Cell Sci 121:3704-16. 2008
    ..We propose a model to explain the role of flagellum elongation in correct flagellar pocket organisation and function...
  9. ncbi Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43
    Xue Hai Liang
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Mol Biochem Parasitol 145:29-39. 2006
    ..We identified a 45S complex carrying pre-mRNA and all the U-snRNAs, including U1 and the SL RNA, suggesting that a single spliceosomal complex may potentially conduct both trans- and cis-splicing...
  10. pmc Novel roles for the flagellum in cell morphogenesis and cytokinesis of trypanosomes
    Linda Kohl
    INSERM U565 and CNRS UMR8646, Laboratoire de Biophysique, Museum national d histoire naturelle, 43 rue Cuvier, 75231 Paris Cedex 05, France
    EMBO J 22:5336-46. 2003
    ..We show that flagellum elongation controls formation of cytoskeletal structures (present in the cell body) that act as molecular organizers of the cell...
  11. ncbi Microtubules, tubulin, and microtubule-associated proteins of trypanosomes
    D Robinson
    Methods Enzymol 196:285-99. 1991
  12. ncbi Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
    Tansy C Hammarton
    Wellcome Centre for Molecular Parasitology, Anderson College, University of Glasgow, 56 Dumbarton Road, Glasgow, G11 6NU, United Kingdom
    J Biol Chem 278:22877-86. 2003
    ..This indicates that there are fundamental differences in cell cycle controls between life cycle forms of T. brucei and that key cell cycle checkpoints present in higher eukaryotes are absent from trypanosomes...
  13. pmc Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor
    Jeffrey A DeGrasse
    Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10065, USA
    Mol Cell Proteomics 8:2119-30. 2009
    ..These findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes and that both were established very early in eukaryotic evolution...
  14. pmc The F(0)F(1)-ATP synthase complex contains novel subunits and is essential for procyclic Trypanosoma brucei
    Alena Zíková
    Seattle Biomedical Research Institute, Seattle, WA, USA
    PLoS Pathog 5:e1000436. 2009
    ..Hence, the two novel proteins appear essential for the structural organization of the functional complex and regulation of mitochondrial energy generation in these organisms is more complicated than previously thought...
  15. ncbi Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite
    J D Barry
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Scotland, UK
    Adv Parasitol 49:1-70. 2001
    ....
  16. pmc Developmental progression to infectivity in Trypanosoma brucei triggered by an RNA-binding protein
    Nikolay G Kolev
    Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06536, USA
    Science 338:1352-3. 2012
    ..Thus, events leading to acquisition of infectivity in the insect vector are now accessible to laboratory investigation, providing an opening for new intervention strategies...
  17. ncbi Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins
    N Ismaili
    Department of Molecular Biology, Free University of Brussels, Rhode St Genese, Belgium
    Mol Biochem Parasitol 102:103-15. 1999
    ..These and other observations suggest that TSR1 may be involved in trans-splicing in T. brucei...
  18. pmc Dual targeting of isoleucyl-tRNA synthetase in Trypanosoma brucei is mediated through alternative trans-splicing
    Jochen Rettig
    Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland
    Nucleic Acids Res 40:1299-306. 2012
    ..In contrast, the shorter spliced variant is translated to a cytosol-specific isoform lacking the presequence. Furthermore, we show that RNA stability is one mechanism determining the differential abundance of the two spliced isoforms...
  19. pmc An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote
    Mauro Serricchio
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Proc Natl Acad Sci U S A 109:E954-61. 2012
    ..During depletion of cardiolipin synthase, the levels of cytochrome oxidase subunit IV and cytochrome c1, reflecting mitochondrial respiratory complexes IV and III, respectively, decreased progressively...
  20. ncbi An easily dissociated 26 S proteasome catalyzes an essential ubiquitin-mediated protein degradation pathway in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:15486-98. 2002
    ..The T. brucei 11 S regulator (PA26)-deficient RNA interference cells grew normally, suggesting the dispensability of activated 20 S proteasome in T. brucei...
  21. pmc Trypanosoma brucei RNA binding proteins p34 and p37 mediate NOPP44/46 cellular localization via the exportin 1 nuclear export pathway
    Kristina Hellman
    Department of Microbiology and Immunology and The Witebsky Center for Microbial Pathogenesis and Immunology, 253 Biomedical Research Building, University at Buffalo, Buffalo, New York 14214, USA
    Eukaryot Cell 6:2206-13. 2007
    ..Together, our results demonstrate that p34 and p37 regulate NOPP44/46 cellular localization by facilitating their association with exportin 1...
  22. pmc Differential trypanosome surface coat regulation by a CCCH protein that co-associates with procyclin mRNA cis-elements
    Pegine Walrad
    Centre for Immunology, Infection and Evolution, Institute of Immunology and Infection Research, School of Biological Sciences, Ashworth Laboratories, University of Edinburgh, Edinburgh, UK
    PLoS Pathog 5:e1000317. 2009
    ..Combined with the known association of TbZFP3 with the translational apparatus, this study provides a long-sought missing link between surface protein cis-regulatory signals and the gene expression machinery in trypanosomes...
  23. pmc A histone methyltransferase modulates antigenic variation in African trypanosomes
    Luisa M Figueiredo
    Laboratory of Molecular Parasitology, Rockefeller University, New York, New York, USA
    PLoS Biol 6:e161. 2008
    ..We conclude that DOT1B is required to maintain strict VSG silencing and to ensure rapid transcriptional VSG switching, demonstrating that epigenetics plays an important role in regulating antigenic variation in T. brucei...
  24. ncbi Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein
    T Nozaki
    Laboratory of Molecular Parasitology, Rockefeller University, New York, NY 10021 6399, USA
    Mol Biochem Parasitol 82:245-55. 1996
    ..Fla1 from both life-cycle stages is N-glycosylated. Fla1 from bloodstream-form T. brucei contains additional glycans, which can be liberated by treatment with mild acid, suggestive of phosphodiester linkages...
  25. ncbi The role of phosphomannose isomerase in Leishmania mexicana glycoconjugate synthesis and virulence
    A Garami
    Max Planck Institut fur Biologie, Corrensstrasse 38, Tubingen 72076, Federal Republic of Germany
    J Biol Chem 276:6566-75. 2001
    ..L. mexicana Delta lmexpmi provides the first conditional mannose-controlled system for parasite glycoconjugate assembly with potential applications for the investigation of their biosynthesis, intracellular sorting, and function...
  26. pmc Interacting protein kinases involved in the regulation of flagellar length
    Maja Erdmann
    Bernhard Nocht Institute for Tropical Medicine, Parasitology Section, D 20359 Hamburg, Germany
    Mol Biol Cell 17:2035-45. 2006
    ..This is the first time that two interacting components of a signaling cascade have been described in the genus Leishmania. Moreover, we set the stage for the analysis of reversible phosphorylation in flagellar morphogenesis...
  27. pmc The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream-form Trypanosoma brucei is dependent on the de novo synthesis of inositol
    Kirstee L Martin
    Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Mol Microbiol 61:89-105. 2006
    ..These results suggest that there is a distinction between de novo synthesized myo-inositol and that from the extracellular environment...
  28. ncbi Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei
    Anton Horváth
    Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
    Mol Microbiol 58:116-30. 2005
    ..The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain...
  29. ncbi Mitochondrial fatty acid synthesis in Trypanosoma brucei
    Jennifer L Stephens
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 282:4427-36. 2007
    ..Trypanosomes employ two FAS systems: the unconventional ELO pathway that synthesizes bulk fatty acids and a mitochondrial pathway that synthesizes specialized fatty acids that are likely utilized intramitochondrially...
  30. pmc Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation
    Chi Ho Li
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, D 69120 Heidelberg, Germany
    RNA 12:2171-86. 2006
    ..We conclude that in trypanosomes 5'-->3' exonuclease activity is important in degradation of highly unstable, regulated mRNAs, but that for other mRNAs another step is more important in determining the decay rate...
  31. ncbi Functional analysis of Trypanosoma brucei PUF1
    Van Duc Luu
    ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Mol Biochem Parasitol 150:340-9. 2006
    ..Alternatively, PUF proteins may be needed in differentiating trypanosomes or in non-culturable life-cycle stages...
  32. ncbi Biochemical characterization of Trypanosoma brucei RNA polymerase II
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, NJ 07103, USA
    Mol Biochem Parasitol 150:201-10. 2006
    ..brucei RNA polymerase II. While both bioinformatics and biochemical analysis have focused on the subunit structure of trypanosome RNA polymerases, this is the first study that reveals a functional RNA polymerase II enzyme...
  33. ncbi Selective di- or trimethylation of histone H3 lysine 76 by two DOT1 homologs is important for cell cycle regulation in Trypanosoma brucei
    Christian J Janzen
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10021, USA
    Mol Cell 23:497-507. 2006
    ..We propose that DOT1A and DOT1B influence the trypanosome cell cycle by regulating the degree of H3K76 methylation...
  34. ncbi Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei
    Claudia Colasante
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Mol Biochem Parasitol 151:193-204. 2007
    ..We also found that production of mRNAs using T7 polymerase can affect the apparent half-life, and that large amounts of CAT enzyme may be toxic in trypanosomes...
  35. pmc Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH
    Ju Huck Lee
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Eukaryot Cell 6:641-9. 2007
    ..Since we also identified orthologues of the TFIIH subunits XPB, p52/TFB2, and p44/SSL1 copurifying with TbXPD, we concluded that the parasite harbors a TFIIH which is indispensable for SL RNA gene transcription...
  36. ncbi Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    J Cell Sci 120:3883-94. 2007
    ..In summary, TLK1 cooperates with Aurora kinase to regulate spindle assembly and chromosome segregation, and it performs a role in DNA replication probably by regulating histone modification in trypanosomes...
  37. pmc Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes
    Neta Milman
    Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 104:19250-5. 2007
    ....
  38. pmc p166, a link between the trypanosome mitochondrial DNA and flagellum, mediates genome segregation
    Zhixing Zhao
    Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA
    EMBO J 27:143-54. 2008
    ..Thus, p166 is the first reported molecular component of the TAC, and its discovery will facilitate study of kDNA segregation machinery at the molecular level...
  39. pmc Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei
    Kristin L Patrick
    Department of Epidemiology and Public Health, Yale University Medical School, 295 Congress Avenue, New Haven, CT 06536 0812, USA
    Mol Microbiol 67:435-47. 2008
    ..We speculate that the small SLACS transcripts serve as substrates for the production of siRNAs to regulate SLACS expression...
  40. ncbi Mitochondrial complexes in Trypanosoma brucei: a novel complex and a unique oxidoreductase complex
    Aswini K Panigrahi
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Mol Cell Proteomics 7:534-45. 2008
    ..The relationship to divergent physiological processes in these pathogens is discussed...
  41. pmc Intraflagellar transport and functional analysis of genes required for flagellum formation in trypanosomes
    Sabrina Absalon
    Trypanosome Cell Biology Unit, Pasteur Institute and Centre National de la Recherche Scientifique, 75015 Paris, France
    Mol Biol Cell 19:929-44. 2008
    ..Two PIFT proteins turned out to be required for retrograde transport and three for anterograde transport. Finally, flagellum membrane elongation continues despite the absence of axonemal microtubules in all IFT/PIFT mutant...
  42. pmc Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e2354. 2008
    ....
  43. pmc Chemical and genetic validation of dihydrofolate reductase-thymidylate synthase as a drug target in African trypanosomes
    Natasha Sienkiewicz
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, UK
    Mol Microbiol 69:520-33. 2008
    ..These data demonstrate that DHFR-TS is essential for parasite survival and represents a promising target for drug discovery...
  44. doi Functional characterization of cohesin SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei
    Mohamed Bessat
    Department of Biological Sciences, University of Hull, Hull, UK
    Mol Microbiol 71:1371-85. 2009
    ....
  45. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
    ..These results suggest that the stabilities of other glycosylphosphatidylinositol pathway enzymes are not dependent on GlcNAc-PI de-N-acetylase levels...
  46. ncbi Molecular characterization of two invariant surface glycoproteins specific for the bloodstream stage of Trypanosoma brucei
    K Ziegelbauer
    Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Tubingen, Federal Republic of Germany
    J Biol Chem 267:10797-803. 1992
    ..They can be detected in all T. brucei brucei variant clones investigated. Both polypeptides are distributed over the entire surface of the parasite...
  47. ncbi Purification and characterization of the membrane-form variant surface glycoprotein hydrolase of Trypanosoma brucei
    R Bülow
    J Biol Chem 261:11918-23. 1986
    ..Reconstitution experiments into phospholipid vesicles show that the enzyme can hydrolyze mfVSG when present in the same phospholipid bilayer but not when present in separate bilayers...
  48. ncbi Amplification of the inosinate dehydrogenase gene in Trypanosoma brucei gambiense due to an increase in chromosome copy number
    K Wilson
    Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201 3098
    J Biol Chem 269:28979-87. 1994
    ..brucei become resistant to cytotoxic drugs, and the amplification of the 6.0-Mb chromosome represents a novel mechanism of drug resistance in parasitic protozoa...
  49. ncbi Translational control mediates the developmental regulation of the Trypanosoma brucei Nrk protein kinase
    M Gale
    Department of Pathobiology, University of Washington, Seattle 98195
    J Biol Chem 269:31659-65. 1994
    ..Thus, Nrk is under translational control. The strict developmental regulation of the Nrk enzymes within the trypanosome life cycle suggests that the Nrk protein kinase may play a role in parasite differentiation...
  50. ncbi A major tyrosine-phosphorylated protein of Trypanosoma brucei is a nucleolar RNA-binding protein
    A Das
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Biol Chem 271:15675-81. 1996
    ..These studies suggest that Nopp44/46 may play a role in RNA metabolism in trypanosomes and raise the possibility that tyrosine phosphorylation may regulate the process...
  51. ncbi Paraflagellar rod is vital for trypanosome motility
    P Bastin
    Nature 391:548. 1998
  52. ncbi Developmental regulation of two nuclear RNA binding proteins, p34 and p37, from Trypanosoma brucei
    J Zhang
    Department of Microbiology, State University of New York at Buffalo, 14214, USA
    Mol Biochem Parasitol 92:79-88. 1998
    ..In bloodstream form p37 protein does correlate with the relative abundance of the steady state mRNA level. The two proteins have been localized to the nucleus by immunofluorescent confocal microscopy and subcellular fractionation...
  53. pmc The kinetoplast structure-specific endonuclease I is related to the 5' exo/endonuclease domain of bacterial DNA polymerase I and colocalizes with the kinetoplast topoisomerase II and DNA polymerase beta during replication
    M L Engel
    Molecular Biology Institute, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095 1570, USA
    Proc Natl Acad Sci U S A 96:8455-60. 1999
    ....
  54. pmc Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei
    K Nagamune
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:10336-41. 2000
    ..Infectivity to tsetse flies is partially impaired, particularly in the early stage. Therefore, parasitespecific inhibition of GPI biosynthesis should be an effective chemotherapy target against African trypanosomiasis...
  55. pmc The surface coat of procyclic Trypanosoma brucei: programmed expression and proteolytic cleavage of procyclin in the tsetse fly
    A Acosta-Serrano
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 98:1513-8. 2001
    ..These findings suggest that one function of the protease-resistant C-terminal domain, containing the amino acid repeats, is to protect the parasite surface from digestive enzymes in the tsetse fly gut...
  56. pmc The exosome of Trypanosoma brucei
    A M Estevez
    Zentrum fur Molekulare Biologie, Heidelberg Universitat, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    EMBO J 20:3831-9. 2001
    ..8S rRNA maturation. We suggest that the exosome was present in primitive eukaryotes, and became increasingly complex during subsequent evolution...
  57. ncbi Isolation and characterization of subnuclear compartments from Trypanosoma brucei. Identification of a major repetitive nuclear lamina component
    M P Rout
    Laboratory of Cellular and Structural Biology, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 276:38261-71. 2001
    ..Immunoelectron microscopy localized NUP-1 to the inner face of the nuclear envelope, suggesting that it is a major filamentous component of the trypanosome nuclear lamina...
  58. pmc Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
    Timothy H P Tan
    Department of Biology Zoology, University of Fribourg, Chemin du Musee 10, CH 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 99:1152-7. 2002
    ..Whereas the predicted protein is homologous to prokaryotic and mitochondrial methionyl-tRNA(Met) formyltransferases, it has about twice the mass of any of these proteins...
  59. ncbi Functional characterization of the 11 non-ATPase subunit proteins in the trypanosome 19 S proteasomal regulatory complex
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:42686-93. 2002
    ..This structural dispensability but functional indispensability of Rpn10 may constitute another unique aspect of the proteasomes in T. brucei...
  60. pmc Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system
    Katherine S Ralston
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 609 Charles E Young Dr, Los Angeles, CA 90095, USA
    Eukaryot Cell 5:696-711. 2006
    ....
  61. ncbi Eubacterial HslV and HslU subunits homologs in primordial eukaryotes
    Bernard Couvreur
    Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Antwerp, Belgium
    Mol Biol Evol 19:2110-7. 2002
    ..To our knowledge this is the first report of a eubacterial HslVU complex in eukaryotes and, consequently, of the simultaneous occurrence of both a proteasome and HslVU in living cells...
  62. ncbi A putative novel nuclear-encoded subunit of the cytochrome c oxidase complex in trypanosomatids
    Dmitri A Maslov
    Department of Biology, University of California, Riverside, CA 92521, USA
    Mol Biochem Parasitol 125:113-25. 2002
    ..brucei and promastigote Leishmania mexicana amazonensis. However, the trCOIV gene, the mRNA and the polypeptide could not be detected in a respiration-deficient trypanosomatid Phytomonas serpens...
  63. ncbi A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 278:20652-8. 2003
    ..This observation indicates an uncoupling between the kinetoplast and the nuclear cycle, resulting in cell division driven by kinetoplast segregation with neither a priori S phase nor mitosis in the trypanosome...
  64. ncbi Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins
    Juliette Moyersoen
    Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, Brussels, Belgium
    Eur J Biochem 270:2059-67. 2003
    ..The RNA interference experiments also showed that TbPEX14 is essential for the survival of bloodstream-form and procyclic trypanosomes. These data indicate the protein's great potential as a target for selective trypanocidal drugs...
  65. ncbi tbCPSF30 depletion by RNA interference disrupts polycistronic RNA processing in Trypanosoma brucei
    Edward F Hendriks
    School of Biological Sciences, Division of Biochemistry, 2 205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:26870-8. 2003
    ..This study is the first to identify and characterize a core component of the T. brucei CPSF and show its involvement in polycistronic RNA processing...
  66. ncbi The roles of intersubunit interactions in exosome stability
    Antonio M Estevez
    Zentrum fur Molekulare Biologie, Heidelberg Universitat, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    J Biol Chem 278:34943-51. 2003
    ..Our results are partially consistent with the proposed model of the exosome, but indicate a different arrangement of the RNase PH proteins...
  67. ncbi Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes
    Tina T Saxowsky
    Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 278:49095-101. 2003
    ....
  68. pmc Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor
    Helena Webb
    Department of Biochemistry 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Nucleic Acids Res 33:1503-12. 2005
    ..Thus, the effect of protein synthesis inhibitors in stabilizing the GPI-PLC mRNA operates in trans through a short-lived factor dependent on protein synthesis...
  69. pmc Mitochondrial DNA ligases of Trypanosoma brucei
    Nick Downey
    Molecular Biology Institute and Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095 1570, USA
    Eukaryot Cell 4:765-74. 2005
    ..Closely related pairs of mitochondrial DNA ligase genes were also identified in Leishmania major and Crithidia fasciculata...
  70. ncbi Metabolism and functions of trypanothione in the Kinetoplastida
    A H Fairlamb
    Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, United Kingdom
    Annu Rev Microbiol 46:695-729. 1992
    ....
  71. ncbi The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei
    K L Martin
    Division of Biological Chemistry and Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem Soc Trans 33:983-5. 2005
    ..brucei and that the de novo synthesized myo-inositol is used for the formation of PI and GPI anchors...
  72. pmc ATP synthase is responsible for maintaining mitochondrial membrane potential in bloodstream form Trypanosoma brucei
    Silvia V Brown
    Department of Microbiology and Immunology, 253 Biomedical Research Building, University at Buffalo, Buffalo, New York 14214, USA
    Eukaryot Cell 5:45-53. 2006
    ..These results support the role of the ATP synthase in the maintenance of the mitochondrial membrane potential in bloodstream form T. brucei...
  73. pmc A "holistic" kinesin phylogeny reveals new kinesin families and predicts protein functions
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Biol Cell 17:1734-43. 2006
    ..Finally, we present a set of hidden Markov models that can reliably place most new kinesin sequences into families, even when from an organism at a great evolutionary distance from those in the analysis...

Research Grants7

  1. EXPANDED PROTEIN STRUCTURE REFINEMENT AND VALIDATION
    Ethan Merritt; Fiscal Year: 2005
    ..This, in turn, will make it easier to identify key biological features such as the presence and conformation of bound ligands, and the nature of hinge and inter-domain motions. ..
  2. Software tools for analysis and visulaization of protein structure
    Ethan A Merritt; Fiscal Year: 2010
    ..The algorithms and computation tools developed as part of this work will be distributed freely, and we will provide web-based state of the art analysis of structures submitted by external researchers. ..
  3. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
  4. A High Throughput Assay to Identify Onhibitors of Trypanosome RNA Editing Ligase
    Nancy Ernst; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  5. A NOVEL MICROTUBULE-ASSOCIATED PROTEIN IN TRYPANOSOMES
    KENT HILL; Fiscal Year: 2002
    ....
  6. MITOCHONDRIAL PROTEIN IMPORT IN AFRICAN TRYPANOSOMES
    Minu Chaudhuri; Fiscal Year: 2002
    ..Understanding of this developmental process will elucidate one of the major aspects of trypanosomal parasitism. This in turn will help us to design rational trypanocidal chemotherapeutic strategies. ..
  7. Biochemical and Functional Analysis of Trypanin
    KENT HILL; Fiscal Year: 2007
    ..Therefore, in addition to addressing fundamental questions in cell biology, this research directly impacts efforts to understand and treat infectious diseases and genetic diseases in humans. ..