Dengue virus 2

Summary

Alias: Dengue virus type 2, dengue 2 virus DEN-2, dengue-2 virus, dengue-2 virus DEN-2, Dengue virus type II

Top Publications

  1. Selisko B, Dutartre H, Guillemot J, Debarnot C, Benarroch D, Khromykh A, et al. Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases. Virology. 2006;351:145-58 pubmed publisher
    ..This suggests that they display a higher conformational flexibility upon the transition from initiation to elongation...
  2. Chiu M, Yang Y. Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli. Biochem Biophys Res Commun. 2003;309:672-8 pubmed
    ....
  3. Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol. 2005;3:13-22 pubmed publisher
    ..This review focuses mainly on structural studies of dengue virus...
  4. Smith K, Nanda K, Spears C, Ribeiro M, Vancini R, Piper A, et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J. 2011;8:289 pubmed publisher
    ..This method for creating live, attenuated viral mutants that generate safe and effective immunity may be applied to many other insect-borne viral diseases for which no current effective therapies exist...
  5. Alcon LePoder S, Drouet M, Roux P, Frenkiel M, Arborio M, Durand Schneider A, et al. The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity. J Virol. 2005;79:11403-11 pubmed publisher
    ....
  6. Puttikhunt C, Kasinrerk W, Srisa ad S, Duangchinda T, Silakate W, Moonsom S, et al. Production of anti-dengue NS1 monoclonal antibodies by DNA immunization. J Virol Methods. 2003;109:55-61 pubmed
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  7. Chung K, Liszewski M, Nybakken G, Davis A, Townsend R, Fremont D, et al. West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H. Proc Natl Acad Sci U S A. 2006;103:19111-6 pubmed publisher
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  8. Mercado Curiel R, Esquinca Avil s H, Tovar R, D az Badillo A, Camacho Nuez M. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006;6:85 pubmed publisher
    ..aegypti midgut. Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells...
  9. MacKenzie J, Jones M, Young P. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 1996;220:232-40 pubmed publisher
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  10. Wang S, He R, Anderson R. PrM- and cell-binding domains of the dengue virus E protein. J Virol. 1999;73:2547-51 pubmed
    ....

Detail Information

Publications84

  1. Selisko B, Dutartre H, Guillemot J, Debarnot C, Benarroch D, Khromykh A, et al. Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases. Virology. 2006;351:145-58 pubmed publisher
    ..This suggests that they display a higher conformational flexibility upon the transition from initiation to elongation...
  2. Chiu M, Yang Y. Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli. Biochem Biophys Res Commun. 2003;309:672-8 pubmed
    ....
  3. Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol. 2005;3:13-22 pubmed publisher
    ..This review focuses mainly on structural studies of dengue virus...
  4. Smith K, Nanda K, Spears C, Ribeiro M, Vancini R, Piper A, et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J. 2011;8:289 pubmed publisher
    ..This method for creating live, attenuated viral mutants that generate safe and effective immunity may be applied to many other insect-borne viral diseases for which no current effective therapies exist...
  5. Alcon LePoder S, Drouet M, Roux P, Frenkiel M, Arborio M, Durand Schneider A, et al. The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity. J Virol. 2005;79:11403-11 pubmed publisher
    ....
  6. Puttikhunt C, Kasinrerk W, Srisa ad S, Duangchinda T, Silakate W, Moonsom S, et al. Production of anti-dengue NS1 monoclonal antibodies by DNA immunization. J Virol Methods. 2003;109:55-61 pubmed
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  7. Chung K, Liszewski M, Nybakken G, Davis A, Townsend R, Fremont D, et al. West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H. Proc Natl Acad Sci U S A. 2006;103:19111-6 pubmed publisher
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  8. Mercado Curiel R, Esquinca Avil s H, Tovar R, D az Badillo A, Camacho Nuez M. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006;6:85 pubmed publisher
    ..aegypti midgut. Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells...
  9. MacKenzie J, Jones M, Young P. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 1996;220:232-40 pubmed publisher
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  10. Wang S, He R, Anderson R. PrM- and cell-binding domains of the dengue virus E protein. J Virol. 1999;73:2547-51 pubmed
    ....
  11. Flamand M, Megret F, Mathieu M, Lepault J, Rey F, Deubel V. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol. 1999;73:6104-10 pubmed
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  12. Westaway E, Khromykh A, Kenney M, MacKenzie J, Jones M. Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus. Virology. 1997;234:31-41 pubmed publisher
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  13. Libraty D, Young P, Pickering D, Endy T, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis. 2002;186:1165-8 pubmed publisher
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  14. Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan K, et al. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis. 2006;193:1078-88 pubmed publisher
    ..Complement activation mediated by NS1 leads to local and systemic generation of anaphylatoxins and SC5b-9, which may contribute to the pathogenesis of the vascular leakage that occurs in patients with DHF/DSS...
  15. Ackermann M, Padmanabhan R. De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase. J Biol Chem. 2001;276:39926-37 pubmed
    ..A model is proposed that a specific conformation of the viral polymerase and/or structure at the 3' end of the template RNA is required for de novo initiation. ..
  16. Kuhn R, Zhang W, Rossmann M, Pletnev S, Corver J, Lenches E, et al. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell. 2002;108:717-25 pubmed
    ..The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane. ..
  17. Falconar A. Identification of an epitope on the dengue virus membrane (M) protein defined by cross-protective monoclonal antibodies: design of an improved epitope sequence based on common determinants present in both envelope (E and M) proteins. Arch Virol. 1999;144:2313-30 pubmed
    ..These peptide sequence will now be tested for their ability to generate cross-protective antibodies against each dengue virus serotype when delivered with appropriate T-helper epitopes. ..
  18. Nomaguchi M, Ackermann M, Yon C, You S, Padmanabhan R, Padmanbhan R. De novo synthesis of negative-strand RNA by Dengue virus RNA-dependent RNA polymerase in vitro: nucleotide, primer, and template parameters. J Virol. 2003;77:8831-42 pubmed
    ....
  19. Bartelma G, Padmanabhan R. Expression, purification, and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 nonstructural protein 3. Virology. 2002;299:122-32 pubmed
    b>Dengue virus type 2 (DEN2), a member of the Flaviviridae family of positive-strand RNA viruses, contains a single RNA genome having a type I cap structure at the 5' end...
  20. Tassaneetrithep B, Burgess T, Granelli Piperno A, Trumpfheller C, Finke J, Sun W, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med. 2003;197:823-9 pubmed
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection. ..
  21. Schlesinger J, Brandriss M, Walsh E. Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. J Gen Virol. 1987;68 ( Pt 3):853-7 pubmed
    Immunization of mice with the dengue 2 virus (DEN 2)-specified non-structural protein NS1 provided significant protection against intracerebral challenge with the virus in the absence of detectable neutralizing or other anti-virion ..
  22. Kanai R, Kar K, Anthony K, Gould L, Ledizet M, Fikrig E, et al. Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes. J Virol. 2006;80:11000-8 pubmed
    ..By revealing the details of the molecular landscape of the West Nile virus surface, our structure will assist the design of antiviral vaccines and therapeutics. ..
  23. Bielefeldt Ohmann H. Analysis of antibody-independent binding of dengue viruses and dengue virus envelope protein to human myelomonocytic cells and B lymphocytes. Virus Res. 1998;57:63-79 pubmed
    ....
  24. Kinney R, Butrapet S, Chang G, Tsuchiya K, Roehrig J, Bhamarapravati N, et al. Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. Virology. 1997;230:300-8 pubmed
    ..Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice. ..
  25. Yap T, Xu T, Chen Y, Malet H, Egloff M, Canard B, et al. Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution. J Virol. 2007;81:4753-65 pubmed
    ..In the absence of a template strand, a chain-terminating nucleoside analogue binds to the priming loop site. These results should inform and accelerate the structure-based design of antiviral compounds against dengue virus. ..
  26. Filomatori C, Iglesias N, Villordo S, Alvarez D, Gamarnik A. RNA sequences and structures required for the recruitment and activity of the dengue virus polymerase. J Biol Chem. 2011;286:6929-39 pubmed publisher
    ..We propose that the long range RNA-RNA interactions in the viral genome play multiple roles during RNA synthesis. Together, we provide new molecular details about the promoter-dependent dengue virus RNA polymerase activity. ..
  27. Egloff M, Benarroch D, Selisko B, Romette J, Canard B. An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. EMBO J. 2002;21:2757-68 pubmed
    ..The crystal structure of an N-terminal fragment of Dengue virus type 2 NS5 is reported at 2.4 A resolution...
  28. Xu T, Sampath A, Chao A, Wen D, Nanao M, Chene P, et al. Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A. J Virol. 2005;79:10278-88 pubmed
    ..A possible model for the unwinding mechanism is proposed. ..
  29. Hung S, Lee P, Chen H, Chen L, Kao C, King C. Analysis of the steps involved in Dengue virus entry into host cells. Virology. 1999;257:156-67 pubmed
    ..We found that three MAbs, 17-2, 46-9, and 51-3, may neutralize dengue 2 virus (DEN-2) through inhibition of not only viral attachment but also of penetration. However, one MAb, 56-3...
  30. Modis Y, Ogata S, Clements D, Harrison S. Structure of the dengue virus envelope protein after membrane fusion. Nature. 2004;427:313-9 pubmed
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry. ..
  31. Sakoonwatanyoo P, Boonsanay V, Smith D. Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein. Intervirology. 2006;49:161-72 pubmed
    ..The results suggest that multiple receptors may be used by the dengue virus to enter into insect cells, and that one of these proteins may be a laminin-binding protein. ..
  32. Johansson M, Brooks A, Jans D, Vasudevan S. A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-beta and the viral helicase, NS3. J Gen Virol. 2001;82:735-45 pubmed
    ..The direct interaction of importin-beta with NS5 has implications for the mechanism by which this normally cytoplasmic protein may be targetted to the nucleus. ..
  33. Pryor M, Azzola L, Wright P, Davidson A. Histidine 39 in the dengue virus type 2 M protein has an important role in virus assembly. J Gen Virol. 2004;85:3627-36 pubmed
    ..In this study, the role of the His residue at position 39 in the M protein (M39) of dengue virus type 2 (DENV-2) in the virus life cycle was investigated...
  34. Leitmeyer K, Vaughn D, Watts D, Salas R, Villalobos I, de Chacon -, et al. Dengue virus structural differences that correlate with pathogenesis. J Virol. 1999;73:4738-47 pubmed
    ..This new approach to the study of dengue virus genome differences should better reflect the true composition of viral RNA populations in the natural host and permit their association with pathogenesis. ..
  35. Li H, Clum S, You S, Ebner K, Padmanabhan R. The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids. J Virol. 1999;73:3108-16 pubmed
    NS3 protein of dengue virus type 2 has a serine protease domain within the N-terminal 180 residues. NS2B is required for NS3 to form an active protease involved in processing of the viral polyprotein precursor...
  36. Zhang W, Chipman P, Corver J, Johnson P, Zhang Y, Mukhopadhyay S, et al. Visualization of membrane protein domains by cryo-electron microscopy of dengue virus. Nat Struct Biol. 2003;10:907-12 pubmed
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus. ..
  37. Modis Y, Ogata S, Clements D, Harrison S. A ligand-binding pocket in the dengue virus envelope glycoprotein. Proc Natl Acad Sci U S A. 2003;100:6986-91 pubmed
    ..A crystal structure of the soluble ectodomain of E from dengue virus type 2 reveals a hydrophobic pocket lined by residues that influence the pH threshold for fusion...
  38. Sánchez I, Ruiz B. A single nucleotide change in the E protein gene of dengue virus 2 Mexican strain affects neurovirulence in mice. J Gen Virol. 1996;77 ( Pt 10):2541-5 pubmed
    Eleven clones of dengue virus 2 Mexican strain were selected by the size of their lytic plaques. Nucleotide sequences of the clones producing large plaques (D2ML2, D2ML3 and D2ML4) revealed 11 mutations, 10 of which were silent...
  39. Yu I, Zhang W, Holdaway H, Li L, Kostyuchenko V, Chipman P, et al. Structure of the immature dengue virus at low pH primes proteolytic maturation. Science. 2008;319:1834-7 pubmed publisher
    ..These results suggest a mechanism by which flaviviruses are processed and stabilized in the host cell secretory pathway. ..
  40. Chua J, Ng M, Chow V. The non-structural 3 (NS3) protein of dengue virus type 2 interacts with human nuclear receptor binding protein and is associated with alterations in membrane structure. Virus Res. 2004;102:151-63 pubmed publisher
    ..Using yeast two-hybrid and co-immunoprecipitation analyses, we demonstrated that the NS3 protein of dengue virus type 2 interacted specifically with nuclear receptor binding protein (NRBP), a host cellular protein that ..
  41. Diamond M, Harris E. Interferon inhibits dengue virus infection by preventing translation of viral RNA through a PKR-independent mechanism. Virology. 2001;289:297-311 pubmed publisher
    ..We conclude that IFN prevents DV infection by inhibiting translation of the infectious viral RNA through a novel, PKR-independent mechanism...
  42. Monteiro J, Oliveira M, Dias R, Nacif Marçal L, Feio R, Ferreira S, et al. The antimicrobial peptide HS-1 inhibits dengue virus infection. Virology. 2018;514:79-87 pubmed publisher
    ..In vivo tests display 80% protection against the dengue-2 virus due to the presence of HS-1, which reveals its potential as an antiviral against the DENV. ..
  43. Hou J, Chen T, Chiang Y, Peng J, Yang T, Cheng C, et al. PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication. Viruses. 2017;9: pubmed publisher
    ..It was ecologically important for virus amplification in mosquitoes and transmission to humans. ..
  44. Falconar A, Martinez F. The NS1 glycoprotein can generate dramatic antibody-enhanced dengue viral replication in normal out-bred mice resulting in lethal multi-organ disease. PLoS ONE. 2011;6:e21024 pubmed publisher
    ..The model provides potential for assessing DENV strain pathogenicity and anti-DENV therapies in normal mice...
  45. Kapoor M, Zhang L, Ramachandra M, Kusukawa J, Ebner K, Padmanabhan R. Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J Biol Chem. 1995;270:19100-6 pubmed
    b>Dengue virus type 2, a member of the family Flaviviridae, encodes a single polyprotein precursor consisting of 3391 amino acids residues that is processed to at least 10 mature proteins by host and viral proteases...
  46. Yap T, Chen Y, Xu T, Wen D, Vasudevan S, Lescar J. A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:78-83 pubmed
    ....
  47. Costa S, Paes M, Barreto D, Pinh o A, Barth O, Queiroz J, et al. Protection against dengue type 2 virus induced in mice immunized with a DNA plasmid encoding the non-structural 1 (NS1) gene fused to the tissue plasminogen activator signal sequence. Vaccine. 2006;24:195-205 pubmed publisher
    ..p.) virus inoculations, and in both cases, pcTPANS1-immunized mice were protected. Overall, these results provide further support for the use of such a plasmid in a possible approach for the development of a vaccine against DENV...
  48. George J, Valiant W, Mattapallil M, Walker M, Huang Y, Vanlandingham D, et al. Prior Exposure to Zika Virus Significantly Enhances Peak Dengue-2 Viremia in Rhesus Macaques. Sci Rep. 2017;7:10498 pubmed publisher
    ..Our results provide first in vivo evidence that prior exposure to Zika virus infection can enhance Dengue infection, which has implications for understanding pathogenesis and the development of vaccines. ..
  49. Staropoli I, Frenkiel M, M gret F, Deubel V. Affinity-purified dengue-2 virus envelope glycoprotein induces neutralizing antibodies and protective immunity in mice. Vaccine. 1997;15:1946-54 pubmed
    ....
  50. Valle R, Falgout B. Mutagenesis of the NS3 protease of dengue virus type 2. J Virol. 1998;72:624-32 pubmed
    ..In all, 46 single-amino-acid substitutions were created in a cloned NS2B-NS3 cDNA fragment of dengue virus type 2, and the effect of each mutation on the extent of self-cleavage of the NS2B-NS3 precursor at the NS2B-NS3 ..
  51. Lindenbach B, Rice C. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J Virol. 1999;73:4611-21 pubmed
    ..These findings suggest a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication...
  52. Bryant J, Calvert A, Mesesan K, Crabtree M, Volpe K, Silengo S, et al. Glycosylation of the dengue 2 virus E protein at N67 is critical for virus growth in vitro but not for growth in intrathoracically inoculated Aedes aegypti mosquitoes. Virology. 2007;366:415-23 pubmed publisher
    To determine the importance of dengue 2 virus (DEN2V) envelope (E) protein glycosylation, virus mutants in one or both of the N-linked glycosylation motifs were prepared...
  53. Su H, Lin Y, Yu H, Tsao C, Chen L, Liu Y, et al. The effect of human bcl-2 and bcl-X genes on dengue virus-induced apoptosis in cultured cells. Virology. 2001;282:141-53 pubmed publisher
    ..Thus, through studying the effect of human bcl-2-related genes, our results suggest that DEN infection may trigger target cells to undergo morphologically similar but biochemically distinct apoptotic pathways in a cell-specific manner...
  54. Leung D, Schroder K, White H, Fang N, Stoermer M, Abbenante G, et al. Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors. J Biol Chem. 2001;276:45762-71 pubmed publisher
    Recombinant forms of the dengue 2 virus NS3 protease linked to a 40-residue co-factor, corresponding to part of NS2B, have been expressed in Escherichia coli and shown to be active against para-nitroanilide substrates comprising the P6-P1 ..
  55. Roehrig J. Antigenic structure of flavivirus proteins. Adv Virus Res. 2003;59:141-75 pubmed
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  56. Lin C, Yang C, Tu C, Huang C, Shih Y, Chuang C, et al. A novel tetraspanin C189 upregulated in C6/36 mosquito cells following dengue 2 virus infection. Virus Res. 2007;124:176-83 pubmed publisher
    ..It is presumably beneficial or essential for cell-to-cell spread of the virus due to the role of tetraspanins demonstrated in intercellular adhesion...
  57. Harris E, Holden K, Edgil D, Polacek C, Clyde K. Molecular biology of flaviviruses. Novartis Found Symp. 2006;277:23-39; discussion 40, 71-3, 251-3 pubmed
    ..Although much remains to be elucidated about the molecular biology of flavivirus infection, progress is being made towards defining the cis and trans factors that regulate flavivirus translation and replication...
  58. Clyde K, Harris E. RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication. J Virol. 2006;80:2170-82 pubmed publisher
    ..b>Dengue virus type 2 (DENV2) and other mosquito-borne flaviviruses initiate translation of C from a start codon in a suboptimal ..
  59. Falconar A, Young P. Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1. J Gen Virol. 1991;72 ( Pt 4):961-5 pubmed publisher
    A panel of mouse monoclonal antibodies (MAbs) raised against the non-structural glycoprotein NS1 of dengue 2 virus (PR159) was studied for cross-reactivity with the NS1 protein of other dengue virus serotypes and other members of the ..
  60. Rawlinson S, Pryor M, Wright P, Jans D. Dengue virus RNA polymerase NS5: a potential therapeutic target?. Curr Drug Targets. 2006;7:1623-38 pubmed
    ..This review examines the unique properties of NS5, including its functions, interactions, subcellular localisation and regulation, and looks at ways in which some of these may be exploited in our quest for effective drugs...
  61. Abdul Ahmad S, Palanisamy U, Tejo B, Chew M, Tham H, Syed Hassan S. Geraniin extracted from the rind of Nephelium lappaceum binds to dengue virus type-2 envelope protein and inhibits early stage of virus replication. Virol J. 2017;14:229 pubmed publisher
    ..Our results demonstrate that geraniin has the potential to be developed into an effective antiviral treatment, particularly for early phase dengue viral infection. ..
  62. Bazan J, Fletterick R. Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology. 1989;171:637-9 pubmed
    ..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
  63. Yon C, Teramoto T, Mueller N, Phelan J, Ganesh V, Murthy K, et al. Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase. J Biol Chem. 2005;280:27412-9 pubmed publisher
    b>Dengue virus type 2 (DEN2), a member of the Flaviviridae family, is a re-emerging human pathogen of global significance...
  64. Goncalvez A, Purcell R, LAI C. Epitope determinants of a chimpanzee Fab antibody that efficiently cross-neutralizes dengue type 1 and type 2 viruses map to inside and in close proximity to fusion loop of the dengue type 2 virus envelope glycoprotein. J Virol. 2004;78:12919-28 pubmed publisher
    ..From the locations of these amino acids, Fab 1A5 appears to recognize a novel epitope that has not been mapped before with a flavivirus monoclonal antibody...
  65. Clum S, Ebner K, Padmanabhan R. Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(Pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B. J Biol Chem. 1997;272:30715-23 pubmed
    Polyprotein processing of dengue virus type 2, a positive strand RNA virus, is carried out by the host signal peptidase and a novel two-component viral proteinase of the serine proteinase family, NS2B/NS3(Pro), in the endoplasmic ..
  66. Erbel P, Schiering N, D Arcy A, Renatus M, Kroemer M, Lim S, et al. Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol. 2006;13:372-3 pubmed publisher
    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
  67. Brooks A, Johansson M, John A, Xu Y, Jans D, Vasudevan S. The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-recognized nuclear localization signals. J Biol Chem. 2002;277:36399-407 pubmed publisher
    ..Intriguingly, the bNLS is highly conserved among Dengue and related flaviviruses, implying a general role for the region and importin beta in the infectious cycle...
  68. Preugschat F, Strauss J. Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo. Virology. 1991;185:689-97 pubmed
    ....
  69. Falconar A, Young P. Immunoaffinity purification of native dimer forms of the flavivirus non-structural glycoprotein, NS1. J Virol Methods. 1990;30:323-32 pubmed
    ..In moderately sized virus growth experiments, greater than 1 mg quantities of purified NS1 were obtained...
  70. Winkler G, Randolph V, Cleaves G, Ryan T, Stollar V. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology. 1988;162:187-96 pubmed
    ..It is formed between 20 and 40 min after NS1 is synthesized and before the protein passes the Golgi apparatus. Both soluble and pelletable extracellular NS1 are also found as the high-molecular-weight form...
  71. Brandt W, Chiewslip D, Harris D, Russell P. Partial purification and characterization of a dengue virus soluble complement-fixing antigen. J Immunol. 1970;105:1565-8 pubmed
  72. Livingston P, Kurane I, Dai L, Okamoto Y, Lai C, Men R, et al. Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities. J Immunol. 1995;154:1287-95 pubmed
    ..These results indicate that CD8+ CTL clones that use different TCR and demonstrate two distinct serotype specificities recognize the same 9-mer peptide in the context of HLA-B35...
  73. Thullier P, Lafaye P, M gret F, Deubel V, Jouan A, Mazi J. A recombinant Fab neutralizes dengue virus in vitro. J Biotechnol. 1999;69:183-90 pubmed
    ..e. nanomolar. Fab 4E11 neutralization capacity was found between 8 and 4-times or less lower than that of mAb 4E11, depending on serotypes, thus the Fab could have a smaller antiviral activity than the mAb in vitro...
  74. Goncalvez A, Engle R, St Claire M, Purcell R, LAI C. Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proc Natl Acad Sci U S A. 2007;104:9422-7 pubmed publisher
    ..Importantly, a 9-aa deletion at the N terminus of the CH(2) domain in the Fc region abrogated the enhancing activity...
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    ..Endothelial cell damage may cause vascular leakage that contributes to the pathogenesis of dengue disease...
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    ..Defining the ApoptoM-activated signalling pathways leading to apoptosis will provide the basis for studying how the M protein might play a key role in the fate of the flavivirus-infected cells...
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    ..These results shed new light on the role of NS4A in the DV replication cycle and provide a model of how this protein induces membrane rearrangements and how this property may be regulated...
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    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
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    ....
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    ..7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories...
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    ..The specificities observed for dengue NS2B/NS3 have features in common with the physiological cleavage sites in the dengue polyprotein; however, all sites reveal previously unrecognized suboptimal sequences...
  82. Lin C, Wan S, Cheng H, Lei H, Lin Y. Autoimmune pathogenesis in dengue virus infection. Viral Immunol. 2006;19:127-32 pubmed publisher
    ..Based on these findings, we hypothesize that anti-DV NS1 is involved in the pathogenesis of DF and DHF/DSS, and this may provide important information in dengue vaccine development...
  83. Ho L, Hung L, Weng C, Wu W, Chou P, Lin Y, et al. Dengue virus type 2 antagonizes IFN-alpha but not IFN-gamma antiviral effect via down-regulating Tyk2-STAT signaling in the human dendritic cell. J Immunol. 2005;174:8163-72 pubmed
    ..These observations provide very useful messages with physiological significance in investigation of the pathogenesis, the defense mechanisms of human hosts and the therapeutic considerations in DV infection...
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    ..These DEN virus serotypes are thus heterogeneous in their use of glycosylation sites. We also determined by a lectin-binding assay that the attached carbohydrates for both viruses were likely to be of the high-mannose type...