ankyrins

Summary

Summary: A family of membrane-associated proteins responsible for the attachment of the cytoskeleton. Erythrocyte-related isoforms of ankyrin attach the SPECTRIN cytoskeleton to a transmembrane protein (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE) in the erythrocyte plasma membrane. Brain-related isoforms of ankyrin also exist.

Top Publications

  1. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
  2. ncbi Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1
    Sven Eric Jordt
    Department of Cellular and Molecular Pharmacology University of California, San Francisco, California 94143 2140, USA
    Nature 427:260-5. 2004
  3. ncbi Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues
    V Bennett
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Physiol Rev 81:1353-92. 2001
  4. ncbi Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with adelta/c-fibers and colocalization with trk receptors
    Kimiko Kobayashi
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo 663 8501, Japan
    J Comp Neurol 493:596-606. 2005
  5. ncbi The ankyrin repeat: a diversity of interactions on a common structural framework
    S G Sedgwick
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK NW7 1AA
    Trends Biochem Sci 24:311-6. 1999
  6. ncbi Phospholipase C and protein kinase A mediate bradykinin sensitization of TRPA1: a molecular mechanism of inflammatory pain
    Shenglan Wang
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo 663 8501, Japan
    Brain 131:1241-51. 2008
  7. pmc Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis
    Inaki Iturbe-Ormaetxe
    School of Integrative Biology, The University of Queensland, St Lucia, QLD 4072, Brisbane, Australia
    J Bacteriol 187:5136-45. 2005
  8. ncbi Structure of an IkappaBalpha/NF-kappaB complex
    M D Jacobs
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 95:749-58. 1998
  9. ncbi A selective filter for cytoplasmic transport at the axon initial segment
    Ai Hong Song
    Institute of Neuroscience, State Key Laboratory of Neurobiology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell 136:1148-60. 2009
  10. pmc AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity
    Kristian L Hedstrom
    Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Cell Biol 183:635-40. 2008

Detail Information

Publications300 found, 100 shown here

  1. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
    ..Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol...
  2. ncbi Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1
    Sven Eric Jordt
    Department of Cellular and Molecular Pharmacology University of California, San Francisco, California 94143 2140, USA
    Nature 427:260-5. 2004
    ..These findings identify a cellular and molecular target for the pungent action of mustard oils and support an emerging role for TRP channels as ionotropic cannabinoid receptors...
  3. ncbi Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues
    V Bennett
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Physiol Rev 81:1353-92. 2001
    ..Exciting questions for the future relate to the molecular basis for these pathways and their roles in a clinical context, either as the basis for disease or more positively as therapeutic targets...
  4. ncbi Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with adelta/c-fibers and colocalization with trk receptors
    Kimiko Kobayashi
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo 663 8501, Japan
    J Comp Neurol 493:596-606. 2005
    ....
  5. ncbi The ankyrin repeat: a diversity of interactions on a common structural framework
    S G Sedgwick
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK NW7 1AA
    Trends Biochem Sci 24:311-6. 1999
    ..In particular, they have begun to reveal how a large family of structurally related proteins can interact specifically with such a diverse array of macromolecular targets...
  6. ncbi Phospholipase C and protein kinase A mediate bradykinin sensitization of TRPA1: a molecular mechanism of inflammatory pain
    Shenglan Wang
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo 663 8501, Japan
    Brain 131:1241-51. 2008
    ..Collectively, these results represent a novel mechanism through which bradykinin released in response to tissue inflammation might trigger the sensation of pain by TRPA1 activation...
  7. pmc Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis
    Inaki Iturbe-Ormaetxe
    School of Integrative Biology, The University of Queensland, St Lucia, QLD 4072, Brisbane, Australia
    J Bacteriol 187:5136-45. 2005
    ..In addition, one wMel ANK gene, which is part of an operon, was absent in the wAu genome. These variations are likely to affect the affinity, function, and cellular location of the predicted proteins encoded by these genes...
  8. ncbi Structure of an IkappaBalpha/NF-kappaB complex
    M D Jacobs
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 95:749-58. 1998
    ..The orientation of IkappaBalpha in the complex places its N- and C-terminal regions in appropriate locations for their known regulatory functions...
  9. ncbi A selective filter for cytoplasmic transport at the axon initial segment
    Ai Hong Song
    Institute of Neuroscience, State Key Laboratory of Neurobiology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Cell 136:1148-60. 2009
    ..This selective AIS filtering may contribute to preferential trafficking and segregation of cellular components in polarized neurons...
  10. pmc AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity
    Kristian L Hedstrom
    Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Cell Biol 183:635-40. 2008
    ..Our results demonstrate that the loss of ankG causes axons to acquire the molecular characteristics of dendrites; thus, ankG is required for the maintenance of neuronal polarity and molecular organization of the AIS...
  11. ncbi AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier
    E Kordeli
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 270:2352-9. 1995
    ..The neural-specific ankyrinG polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments...
  12. pmc TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury
    Koichi Obata
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, 1 1 Mukogawa cho, Nishinomiya, Hyogo, Japan
    J Clin Invest 115:2393-401. 2005
    ..Thus, blocking TRPA1 in sensory neurons might provide a fruitful strategy for treating cold hyperalgesia caused by inflammation and nerve damage...
  13. ncbi Novel interactions of ankyrins-G at the costameres: the muscle-specific Obscurin/Titin-Binding-related Domain (OTBD) binds plectin and filamin C
    Yimingjiang Maiweilidan
    INSERM, UMR S 665, Paris, F 75015, France
    Exp Cell Res 317:724-36. 2011
    b>Ankyrins, the adapters of the spectrin skeleton, are involved in local accumulation and stabilization of integral proteins to the appropriate membrane domains...
  14. pmc Cysteine 70 of ankyrin-G is S-palmitoylated and is required for function of ankyrin-G in membrane domain assembly
    Meng He
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 287:43995-4005. 2012
    ....
  15. pmc A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly
    Mauricio R Galiano
    Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 149:1125-39. 2012
    ..Thus, the distal axonal cytoskeleton functions as an intra-axonal boundary restricting ankG to the AIS...
  16. pmc The interaction between L1-type proteins and ankyrins--a master switch for L1-type CAM function
    Michael Hortsch
    Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Mol Biol Lett 14:57-69. 2009
    ..The interaction with ankyrins directs L1-type adhesive proteins into different functional contexts, either ankyrin-independent functions, such ..
  17. pmc Structural basis for spectrin recognition by ankyrin
    Jonathan J Ipsaro
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Blood 115:4093-101. 2010
    ..The structure represents not only the first of a spectrin fragment in complex with its binding partner, but also that of an intermolecular complex involving a ZU5 domain...
  18. ncbi An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan
    Gai Ayalon
    Howard Hughes Medical Institute and Departments of Cell Biology, Biochemistry, and Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Cell 135:1189-200. 2008
    ..Dynactin-4 and a subset of microtubules disappear from sarcolemmal sites in ankyrin-B-depleted muscle. Ankyrin-B thus is an adaptor required for sarcolemmal localization of dystrophin, as well as dynactin-4...
  19. pmc Sensitization of TRPA1 by PAR2 contributes to the sensation of inflammatory pain
    Yi Dai
    Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo, Japan
    J Clin Invest 117:1979-87. 2007
    ....
  20. ncbi Organizing the fluid membrane bilayer: diseases linked to spectrin and ankyrin
    Vann Bennett
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Trends Mol Med 14:28-36. 2008
    ..Together, these studies identify an emerging paradigm for pathogenesis of human disease where failure in cellular localization of membrane-spanning proteins results in loss of physiological function...
  21. pmc AnkyrinG is required to maintain axo-dendritic polarity in vivo
    Jürgen Markus Sobotzik
    Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe University, D 60590 Frankfurt Main, Germany
    Proc Natl Acad Sci U S A 106:17564-9. 2009
    ..Collectively, these findings demonstrate that axons of ankG-deficient mice acquire hallmark features of dendrites. AnkG thus is important for maintaining appropriate axo-dendritic polarity in vivo...
  22. pmc Membrane domains based on ankyrin and spectrin associated with cell-cell interactions
    Vann Bennett
    Howard Hughes Medical Institute, and Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cold Spring Harb Perspect Biol 1:a003012. 2009
    ..This review will summarize basic features of ankyrins and spectrins, and will discuss emerging evidence that these proteins are key players in a conserved mechanism ..
  23. ncbi A targeting motif involved in sodium channel clustering at the axonal initial segment
    Juan Jose Garrido
    Institut National de la Santé et de la Recherche Médicale Unité 464, Institut Jean Roche, Universite de la Mediterranee, Faculte de Medecine Secteur Nord, Boulevard P Dramard, 13916 Marseille Cedex 20, France
    Science 300:2091-4. 2003
    ..Thus, this motif may play a fundamental role in controlling electrical excitability during development and plasticity...
  24. pmc Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization
    Armen N Akopian
    Department of Endodontics, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    J Physiol 583:175-93. 2007
    ..In conclusion, we propose novel mechanisms whereby TRPA1 activity undergoes pharmacological desensitization through multiple cellular pathways that are agonist dependent and modulated by TRPV1...
  25. ncbi Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos
    Krishnakumar Kizhatil
    Howard Hughes Medical Institute, Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:26552-61. 2007
    ..Biol. Chem. 282, 2029-2037 ). Together with the current findings, these data suggest a ankyrin/spectrin-based mechanism for coordinating membrane assembly with extracellular interactions of E-cadherin at sites of cell-cell contact...
  26. pmc HC-030031, a TRPA1 selective antagonist, attenuates inflammatory- and neuropathy-induced mechanical hypersensitivity
    Samer R Eid
    Department of Pain Research, Neuroscience Drug Discovery, Merck Research Laboratories, West Point, Philadelphia, USA
    Mol Pain 4:48. 2008
    ..The current study was designed to further explore the therapeutic potential of pharmacological TRPA1 antagonism to treat inflammatory and neuropathic pain...
  27. pmc Ankyrin-B protein in heart failure: identification of a new component of metazoan cardioprotection
    Farshid Kashef
    Dorothy M Davis Heart and Lung Research Institute, Columbus, OH 43210, USA
    J Biol Chem 287:30268-81. 2012
    b>Ankyrins (ankyrin-R, -B, and -G) are adapter proteins linked with defects in metazoan physiology...
  28. pmc A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells of mice
    Andreas Greth
    The Menzies Research Institute of Tasmania, University of Tasmania, Hobart, Australia
    PLoS ONE 7:e38999. 2012
    ..05). We conclude that increased malaria resistance due to ankyrin-1 deficiency is caused by the intraerythrocytic death of P. chabaudi parasites...
  29. ncbi The CACNA1C and ANK3 risk alleles impact on affective personality traits and startle reactivity but not on cognition or gating in healthy males
    Panos Roussos
    Department of Psychiatry and Behavioral Sciences, University of Crete, Heraklion, Greece
    Bipolar Disord 13:250-9. 2011
    ..The aim of this study was to examine the phenotypic consequences of the risk CACNA1C and ANK3 alleles in a large homogeneous cohort of healthy young males...
  30. pmc Novel roles for erythroid Ankyrin-1 revealed through an ENU-induced null mouse mutant
    Gerhard Rank
    Rotary Bone Marrow Research Laboratory, Melbourne Health Research Directorate, Parkville, Australia
    Blood 113:3352-62. 2009
    ..These findings provide novel insights into the role of Ank-1, and define an ideal model for the study of HS and malarial resistance...
  31. pmc Roles of transient receptor potential channels in pain
    Cheryl L Stucky
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Brain Res Rev 60:2-23. 2009
    ....
  32. pmc Ankyrin binds to two distinct cytoplasmic domains of Na,K-ATPase alpha subunit
    P Devarajan
    Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510
    Proc Natl Acad Sci U S A 91:2965-9. 1994
    ..of the Na,K-ATPase domains, immobilized on a bioaffinity column, also interact specifically with both of these ankyrins. These ATPase domains are encoded by codons 140-290 (domain II) and 345-784 (domain III), with domain II ..
  33. ncbi The molecular basis of hereditary red cell membrane disorders
    Jean Delaunay
    AP HP, Hopital de Bicetre, Service d Hematologie, INSERM Unité 779, Faculte de Medecine Paris Sud, Le Kremlin Bicetre, France
    Blood Rev 21:1-20. 2007
    ..The diagnosis, follow-up and treatment of the involved diseases have gradually improved...
  34. ncbi Selection and characterization of Her2 binding-designed ankyrin repeat proteins
    Christian Zahnd
    Department of Biochemistry, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    J Biol Chem 281:35167-75. 2006
    ....
  35. pmc Identification of a small cytoplasmic ankyrin (AnkG119) in the kidney and muscle that binds beta I sigma spectrin and associates with the Golgi apparatus
    P Devarajan
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Cell Biol 133:819-30. 1996
    b>Ankyrins are a family of large, membrane-associated proteins that mediate the linkage of the cytoskeleton to a variety of membrane transport and receptor proteins...
  36. pmc Na,K-ATPase transport from endoplasmic reticulum to Golgi requires the Golgi spectrin-ankyrin G119 skeleton in Madin Darby canine kidney cells
    P Devarajan
    Department of Pediatrics, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 94:10711-6. 1997
    ....
  37. ncbi Genome-wide association study of bipolar I disorder in the Han Chinese population
    M T M Lee
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    Mol Psychiatry 16:548-56. 2011
    ..55 × 10(-5) and P=1.48 × 10(-5), respectively). This may suggest that there are common and population-specific susceptibility genes for bipolar I disorder...
  38. pmc General anesthetics activate a nociceptive ion channel to enhance pain and inflammation
    JOSE A MATTA
    Departments of Pharmacology and Physiology and Biophysics, Georgetown University, 3900 Reservoir Road, NW, Washington, DC 20007, USA
    Proc Natl Acad Sci U S A 105:8784-9. 2008
    ..Thus, our results show that TRPA1 is essential for sensing noxious GAs. The pronociceptive effects of GAs combined with surgical tissue damage could lead to a paradoxical increase in postoperative pain and inflammation...
  39. ncbi DARPins: a new generation of protein therapeutics
    Michael T Stumpp
    Molecular Partners AG, Grabenstrasse 11a, 8952 Zurich Schlieren, Switzerland
    Drug Discov Today 13:695-701. 2008
    ..Taken together, DARPins are a prominent member of the next generation of protein therapeutics with the potential to surpass existing antibody drugs...
  40. ncbi KCNQ2 is a nodal K+ channel
    Jérôme J Devaux
    Department of Neurology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6077, USA
    J Neurosci 24:1236-44. 2004
    ..The diminished activity of mutant KCNQ2 channels accounts for neonatal epilepsy and myokymia; the cellular locus of these effects may be axonal initial segments and nodes...
  41. pmc Structures of the spectrin-ankyrin interaction binding domains
    Jonathan J Ipsaro
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Blood 113:5385-93. 2009
    ..Previously reported mutants of ankyrin that map to this region lie mostly on the surface of the protein, although at least one is likely to be part of the core...
  42. ncbi The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Curr Biol 15:929-34. 2005
    ..We further show that allicin, an unstable component of fresh garlic, is the chemical responsible for TRPA1 and TRPV1 activation and is therefore likely to cause garlic's pungency...
  43. pmc Variability and expression of ankyrin domain genes in Wolbachia variants infecting the mosquito Culex pipiens
    Olivier Duron
    Institut des Sciences de l Evolution UMR 5554, Université Montpellier II C C 065, F 34095 Montpellier cedex 05, France
    J Bacteriol 189:4442-8. 2007
    ..Further analysis of ANK gene expression evidenced host- and sex-dependent variations, which did not improve the correlation. Taken together, these data do not support the direct implication of ANK genes in CI determinism...
  44. pmc AnkyrinG is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing
    D Zhou
    Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Biol 143:1295-304. 1998
    ..These results demonstrate that ankyrinG is essential for clustering NaCh and neurofascin at axon initial segments and is required for physiological levels of sodium channel activity...
  45. ncbi Sodium channel beta subunits mediate homophilic cell adhesion and recruit ankyrin to points of cell-cell contact
    J D Malhotra
    Department of Pharmacology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 275:11383-8. 2000
    ..It is hypothesized that sodium channel beta subunits serve as a critical communication link between the extracellular and intracellular environments of the neuron and may play a role in sodium channel placement at nodes of Ranvier...
  46. pmc betaIV spectrin, a new spectrin localized at axon initial segments and nodes of ranvier in the central and peripheral nervous system
    S Berghs
    Department of Internal Medicine, Section of Endocrinology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Cell Biol 151:985-1002. 2000
    ..Thus, we suggest that betaIVSigma1 spectrin interacts with ankyrin(G) 480/270-kD and participates in the clustering of voltage-gated Na(+) channels and cell-adhesion molecules at initial segments and nodes of Ranvier...
  47. pmc Genetic analysis of acd6-1 reveals complex defense networks and leads to identification of novel defense genes in Arabidopsis
    Hua Lu
    Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    Plant J 58:401-12. 2009
    ..Taken together, acd6-1 is a powerful tool not only for dissecting defense regulatory networks but also for discovering novel defense genes...
  48. pmc Ankyrin-G coordinates assembly of the spectrin-based membrane skeleton, voltage-gated sodium channels, and L1 CAMs at Purkinje neuron initial segments
    S M Jenkins
    Howard Hughes Medical Institute and Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 155:739-46. 2001
    ....
  49. ncbi Ankyrins and cellular targeting of diverse membrane proteins to physiological sites
    V Bennett
    Howard Hughes Medical Institute and the Departments of Cell Biology and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Opin Cell Biol 13:61-7. 2001
    b>Ankyrins are spectrin-binding proteins that associate via ANK repeats with a variety of ion channels/pumps, calcium release channels and cell adhesion molecules...
  50. ncbi Cytoplasmic domain mutations of the L1 cell adhesion molecule reduce L1-ankyrin interactions
    L K Needham
    Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    J Neurosci 21:1490-500. 2001
    ....
  51. ncbi Spectrins and ankyrinB constitute a specialized paranodal cytoskeleton
    Yasuhiro Ogawa
    Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Neurosci 26:5230-9. 2006
    ..Our results demonstrate that the paranodal junction contains specialized cytoskeletal components that may be important to stabilize axon-glia interactions and contribute to the membrane protein diffusion barrier found at paranodes...
  52. ncbi Structural requirements for association of neurofascin with ankyrin
    X Zhang
    Howard Hughes Medical Institute and Departments of Cell Biology and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:30785-94. 1998
    ..These results provide evidence for a highly specific interaction between ankyrin and neurofascin and suggest that ankyrin association with L1 is required for L1 function in humans...
  53. pmc Ikappabeta-related vankyrin genes in the Campoletis sonorensis ichnovirus: temporal and tissue-specific patterns of expression in parasitized Heliothis virescens lepidopteran hosts
    Jeremy A Kroemer
    University of Kentucky, Department of Entomology, S 225 Agricultural Sciences Center North, Lexington, KY 40546, USA
    J Virol 79:7617-28. 2005
    ..We hypothesize that PDV vankyrin genes may suppress NF-kappabeta activity during immune responses and developmental cascades in parasitized lepidopteran hosts of C. sonorensis...
  54. ncbi Drosophila ankyrin 2 is required for synaptic stability
    Iris Koch
    Max Planck Institute for Developmental Biology, Department III Genetics, Spemannstrasse 35, 72076 Tubingen, Germany
    Neuron 58:210-22. 2008
    ..Interestingly, Ank2 functions downstream of spectrin in the anchorage of synaptic microtubules, providing the cytoskeletal scaffold that is essential for synaptic stability...
  55. pmc Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles
    Paola Bagnato
    Molecular Medicine Section, Department of Neuroscience, University of Siena, 53100 Siena, Italy
    J Cell Biol 160:245-53. 2003
    ..In keeping with the proposed role of obscurin in mediating an interaction with ankyrins and sarcoplasmic reticulum, we have also found that a sequence with homology to the obscurin-binding site of ank1...
  56. ncbi Isoform specificity of ankyrin-B: a site in the divergent C-terminal domain is required for intramolecular association
    Khadar M Abdi
    Howard Hughes Medical Institute, and Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 281:5741-9. 2006
    b>Ankyrins contain significant amino acid identity and are co-expressed in many cell types yet maintain unique functions in vivo...
  57. pmc A novel ENU-generated truncation mutation lacking the spectrin-binding and C-terminal regulatory domains of Ank1 models severe hemolytic hereditary spherocytosis
    Michael R Hughes
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC, Canada
    Exp Hematol 39:305-20, 320.e1-2. 2011
    ..We investigated a novel mouse model in which the heterozygotes present with the diagnostic hallmarks of mild HS and surviving homozygotes phenocopy severe hemolytic HS...
  58. pmc Ankyrin-B coordinates the Na/K ATPase, Na/Ca exchanger, and InsP3 receptor in a cardiac T-tubule/SR microdomain
    Peter J Mohler
    Department of Pathology, Vanderbilt University, Nashville, Tennessee, USA
    PLoS Biol 3:e423. 2005
    ..Ankyrin-B also is not abundantly expressed in smooth muscle. We propose that the ankyrin-B-based complex is a specialized adaptation of cardiomyocytes with a role for cytosolic Ca2+ modulation...
  59. pmc A cardiac arrhythmia syndrome caused by loss of ankyrin-B function
    Peter J Mohler
    Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 101:9137-42. 2004
    ....
  60. pmc Crystal structure of a 12 ANK repeat stack from human ankyrinR
    Peter Michaely
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75235 9039, USA
    EMBO J 21:6387-96. 2002
    b>Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities...
  61. ncbi The axonal localization of large Drosophila ankyrin2 protein isoforms is essential for neuronal functionality
    Michael Hortsch
    Department of Cell and Developmental Biology, University of Michigan, Ann Arbor 48109 0616, USA
    Mol Cell Neurosci 20:43-55. 2002
    ..We show that once polarization is established, it persists during later stages of Drosophila development. We also present genetic evidence that the absence of axonal Dank2 protein is lethal...
  62. pmc Electrostatic interactions mediate binding of obscurin to small ankyrin 1: biochemical and molecular modeling studies
    Ben Busby
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA
    J Mol Biol 408:321-34. 2011
    ..Our results are consistent with a model in which electrostatic interactions between specific pairs of side chains on obscurin and sAnk1 promote binding and complex formation...
  63. ncbi Molecular and genetic evidence for abnormalities in the nodes of Ranvier in schizophrenia
    Panos Roussos
    Department of Psychiatry, The Mount Sinai School of Medicine, Bronx, NY 10468, USA
    Arch Gen Psychiatry 69:7-15. 2012
    ....
  64. pmc Ankyrin G restricts ion channel diffusion at the axonal initial segment before the establishment of the diffusion barrier
    Anna Brachet
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Rercherche 641, Marseille F 13916, France
    J Cell Biol 191:383-95. 2010
    ..In conclusion, our findings indicate that specific binding to ankG constitutes the initial step for Nav channel immobilization at the AIS membrane and precedes the establishment of the diffusion barrier...
  65. ncbi Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation
    Marlyse A Debrincat
    Division of Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Biol Chem 282:4728-37. 2007
    ..The targeting of CKB for degradation by Asb-9 was primarily SOCS box-dependent and suggests that Asb-9 acts as a specific ubiquitin ligase regulating levels of this evolutionarily conserved enzyme...
  66. ncbi Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B
    Peter J Mohler
    Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 279:12980-7. 2004
    ..These new results provide the first physiological evidence of a molecular partner required for early post-translational stability of InsP(3)R...
  67. ncbi Ankyrin-1 mutations are a major cause of dominant and recessive hereditary spherocytosis
    S W Eber
    Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Genet 13:214-8. 1996
    ....
  68. ncbi Defining the cellular phenotype of "ankyrin-B syndrome" variants: human ANK2 variants associated with clinical phenotypes display a spectrum of activities in cardiomyocytes
    Peter J Mohler
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, USA
    Circulation 115:432-41. 2007
    ..More importantly, there is no cellular explanation for the range of severity of cardiac phenotypes associated with specific ANK2 variants...
  69. ncbi Ankyrin-G and beta2-spectrin collaborate in biogenesis of lateral membrane of human bronchial epithelial cells
    Krishnakumar Kizhatil
    Howard Hughes Medical Institute and Departments of Cell Biology, Biochemistry, and Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:2029-37. 2007
    b>Ankyrins are a family of adapter proteins required for localization of membrane proteins to diverse specialized membrane domains including axon initial segments, specialized sites at the transverse tubule/sarcoplasmic reticulum in ..
  70. ncbi Hematologically important mutations: ankyrin variants in hereditary spherocytosis
    Patrick G Gallagher
    Department of Pediatrics, Yale University School of Medicine, 333 Cedar Street, P O Box 208064, New Haven, CT 06520 8064, USA
    Blood Cells Mol Dis 35:345-7. 2005
    ..In most cases, HS-related ankyrin mutations are private. A summary of reported HS-associated ankyrin gene mutations is provided in this report...
  71. pmc Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury
    Gai Ayalon
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 286:7370-8. 2011
    ..Ankyrin-B then interacts with dynactin-4 and dystrophin, whereas dynactin-4 collaborates with dystrophin in coordinating costamere-aligned microtubules...
  72. pmc Voltage-gated ion channels in the axon initial segment of human cortical pyramidal cells and their relationship with chandelier cells
    Maria Carmen Inda
    Departamento de Biologia Celular, Universidad Complutense de Madrid, Jose Antonio Novais 2, 28040 Madrid, Spain
    Proc Natl Acad Sci U S A 103:2920-5. 2006
    ..We suggest that this molecular segregation and the consequent regional specialization of the GABAergic input to the AIS of pyramidal cells may have important functional implications for the control of pyramidal cell activity...
  73. pmc Ankyrin-based cellular pathways for cardiac ion channel and transporter targeting and regulation
    Shane R Cunha
    Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States
    Semin Cell Dev Biol 22:166-70. 2011
    ..b>Ankyrins are a family of multifunctional adapter proteins required for the expression, membrane localization, and ..
  74. pmc Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons
    Mala M Shah
    Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Proc Natl Acad Sci U S A 105:7869-74. 2008
    ..These results indicate that axonal Kv7 channels, by controlling axonal RMP and action potential threshold, are fundamental for regulating the inherent firing properties of CA1 hippocampal neurons...
  75. ncbi Lateral membrane biogenesis in human bronchial epithelial cells requires 190-kDa ankyrin-G
    Krishnakumar Kizhatil
    Howard Hughes Medical Institute and Departments of Cell Biology, Biochemistry, and Neuroscience, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:16706-14. 2004
    ..These results demonstrate that 190-kDa ankyrin-G plays a pleiotropic role in assembly of lateral membranes of bronchial epithelial cells...
  76. pmc Made for "anchorin": Kv7.2/7.3 (KCNQ2/KCNQ3) channels and the modulation of neuronal excitability in vertebrate axons
    Edward C Cooper
    Neurology and Neuroscience, Baylor College of Medicine, Houston, TX 77030, United States
    Semin Cell Dev Biol 22:185-92. 2011
    ..The natural selection for KCNQ2 and KCNQ3 appears to hinge on these subunits' unique ability to be coordinately localized with Na(V) channels by ankyrin-G, and the resulting enhancement in the reliability of neuronal excitability...
  77. ncbi Nanospring behaviour of ankyrin repeats
    Gwangrog Lee
    Department of Mechanical Engineering and Materials Science and Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708, USA
    Nature 440:246-9. 2006
    ..Thus, we show that one of the most common amino-acid motifs has spring properties that could be important in mechanotransduction and in the design of nanodevices...
  78. pmc Perisomatic GABAergic innervation in prefrontal cortex is regulated by ankyrin interaction with the L1 cell adhesion molecule
    Hanjun Guan
    Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Cereb Cortex 20:2684-93. 2010
    ..These results suggest a novel role for L1 engagement with the actin cytoskeleton in development of inhibitory connectivity within the cingulate cortex...
  79. ncbi Normoblastosis, a murine model for ankyrin-deficient hemolytic anemia, is caused by a hypomorphic mutation in the erythroid ankyrin gene Ank1
    Connie S Birkenmeier
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Hematol J 4:445-9. 2003
    ..relative to the murine spectrin-deficient anemias, spherocytosis (Spna1(sph), Spna1(sph-1J), Spna1(sph-2BC), Spna1(sph-DEM)) and jaundiced (Spnb1(ja)), and suggests that truncated ankyrins could be useful in gene replacement therapy.
  80. ncbi Resistance to malaria in ankyrin and spectrin deficient mice
    H L Shear
    Department of Medical and Molecular Parasitology, New York University School of Medicine, N Y
    Br J Haematol 78:555-60. 1991
    ..The heterozygote for the nb defect (nb/+) exhibited a diminution of parasitaemia. We conclude that the host cell spectrin may be necessary for the invasion and/or growth of rodent malarial parasites...
  81. ncbi Evidence for cis-acting regulation of ANK3 and CACNA1C gene expression
    Emma M Quinn
    Neuropsychiatric Genetics Research Group, Department of Psychiatry and Institute of Molecular Medicine, Trinity College Dublin, Ireland
    Bipolar Disord 12:440-5. 2010
    ..We investigated whether, instead of affecting protein function, these risk variants might impact on gene regulation affecting expression...
  82. ncbi Most genome-wide significant susceptibility loci for schizophrenia and bipolar disorder reported to date cross-traditional diagnostic boundaries
    Hywel J Williams
    MRC Centre for Neuropsychiatric Genetics and Genomics, Department of Psychological Medicine and Neurology, School of Medicine, Cardiff University, Cardiff, UK
    Hum Mol Genet 20:387-91. 2011
    ..7 × 10(-5)). Including earlier reported data for trans-disorder effects for ZNF804A and CACNA1C, six out of eight of the most robustly associated loci for either disorder show trans-disorder effects...
  83. pmc Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa
    Christopher T Price
    Department of Microbiology and Immunology, College of Medicine, University of Louisville, Kentucky, USA
    PLoS Pathog 5:e1000704. 2009
    ..Therefore, AnkB exhibits a novel molecular and functional mimicry of eukaryotic F-box proteins that exploits conserved polyubiquitination machinery for intracellular proliferation within evolutionarily distant hosts...
  84. pmc Ankyrin recognizes both surface character and shape of the 14-15 di-repeat of beta-spectrin
    Penelope J La-Borde
    University of Illinois at Chicago, Department of Biochemistry and Molecular Genetics, 900 S Ashland Ave, MBRB 1170, Chicago, IL 60607, USA
    Biochem Biophys Res Commun 392:490-4. 2010
    ..The clinical implications of this are discussed in light of recognized linker mutations and polymorphisms in the beta-spectrins...
  85. ncbi An alternate promoter directs expression of a truncated, muscle-specific isoform of the human ankyrin 1 gene
    P G Gallagher
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520 8021, USA
    J Biol Chem 273:1339-48. 1998
    ..A polyclonal antibody raised to human ankyrin 1 muscle-specific sequences reacted with peptides of 28 and 30 kDa on immunoblots of human skeletal muscle...
  86. ncbi L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection
    Mona Buhusi
    Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Neurosci 28:177-88. 2008
    ....
  87. pmc Gene variants associated with schizophrenia in a Norwegian genome-wide study are replicated in a large European cohort
    Lavinia Athanasiu
    Institute of Psychiatry, University of Oslo, P O 1130, Blindern, N 0318 Oslo, Norway
    J Psychiatr Res 44:748-53. 2010
    ..The association of ANK3 with schizophrenia is intriguing in light of recent associations of ANK3 with bipolar disorder, thereby supporting the hypothesis of an overlap in genetic susceptibility between these psychopathological entities...
  88. pmc The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans
    Lisa Klasson
    Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, SE 752 36 Uppsala, Sweden
    Proc Natl Acad Sci U S A 106:5725-30. 2009
    ..simulans. These results suggest that the transmission dynamics of Wolbachia and the opportunity for coinfections have created a freely recombining intracellular bacterial community with mosaic genomes...
  89. pmc Two variants in Ankyrin 3 (ANK3) are independent genetic risk factors for bipolar disorder
    T G Schulze
    Unit on the Genetic Basis of Mood and Anxiety Disorders, National Institute of Mental Health, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892 3719, USA
    Mol Psychiatry 14:487-91. 2009
    ..Further analysis suggested that each marker contributed independently to BD, with no significant marker x marker interaction. Our findings strongly support ANK3 as a BD susceptibility gene and suggest true allelic heterogeneity...
  90. ncbi Ankyrin-B targets beta2-spectrin to an intracellular compartment in neonatal cardiomyocytes
    Peter J Mohler
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 279:40185-93. 2004
    ..This work also establishes a functional hierarchy in which ankyrin-B determines the localization of beta(2)-spectrin and operates independently of beta(2)-spectrin in its role in organizing membrane-spanning proteins...
  91. pmc How cold is it? TRPM8 and TRPA1 in the molecular logic of cold sensation
    David D McKemy
    Department of Biological Sciences, Neurobiology Section and School of Dentistry, University of Southern California, 925 West 34th Street, Room 4110, Los Angeles, CA 90089 0641, USA
    Mol Pain 1:16. 2005
    ..This review is intended to summarize our current understanding of these cold thermoreceptors, as well as address the current controversy regarding TRPA1 and cold signaling...
  92. pmc Restriction of 480/270-kD ankyrin G to axon proximal segments requires multiple ankyrin G-specific domains
    X Zhang
    Howard Hughes Medical Institute and Departments of Cell Biology and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Biol 142:1571-81. 1998
    ..This complex behavior explains ability of ankyrinB and ankyrinG to sort to distinct sites in neurons and the fact that these ankyrins do not compensate for each other in ankyrin gene knockouts in mice.
  93. ncbi Ankyrins and human disease: what the electrophysiologist should know
    Peter J Mohler
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Cardiovasc Electrophysiol 17:1153-9. 2006
    ..b>Ankyrins are a family of "adaptor" proteins, which play critical roles in the proper expression and membrane localization ..
  94. pmc Localization and structure of the ankyrin-binding site on beta2-spectrin
    Lydia Davis
    Department of Cell Biology and Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:6982-7. 2009
    ..Here, we present the crystal structure of the ankyrin-binding domain of human beta2-spectrin at 1.95 A resolution together with mutagenesis data identifying the binding surface for ankyrins on beta2-spectrin.
  95. ncbi Targeted mutational analysis of ankyrin-B in 541 consecutive, unrelated patients referred for long QT syndrome genetic testing and 200 healthy subjects
    Jonathan Sherman
    Mayo Medical School, 200 First Street SW, Rochester, MN 55905, USA
    Heart Rhythm 2:1218-23. 2005
    ..Mutations in ANK2-encoded ankyrin-B underlie long QT syndrome type 4 (LQT4) and various other dysrhythmia phenotypes...
  96. pmc CD4-specific designed ankyrin repeat proteins are novel potent HIV entry inhibitors with unique characteristics
    Andreas Schweizer
    Division of Infectious Diseases, University Hospital Zurich, Zurich, Switzerland
    PLoS Pathog 4:e1000109. 2008
    ....
  97. ncbi Coinheritance of Gilbert syndrome increases the risk for developing gallstones in patients with hereditary spherocytosis
    E M del Giudice
    Department of Pediatrics, Second University of Naples, Naples, Italy
    Blood 94:2259-62. 1999
    ....
  98. pmc The structure of the ankyrin-binding site of beta-spectrin reveals how tandem spectrin-repeats generate unique ligand-binding properties
    Paul R Stabach
    Department of Pathology, Yale University, New Haven, CT 06520, USA
    Blood 113:5377-84. 2009
    ..The putative coupling of flexibility and ligand binding suggests a mechanism by which spectrin might participate in mechanosensory regulation...
  99. ncbi Association of small ankyrin 1 with the sarcoplasmic reticulum
    Neil C Porter
    Department of Neurology, University of Maryland School of Maryland, Baltimore, MD 21201, USA
    Mol Membr Biol 22:421-32. 2005
    ..It also targets reporter proteins to the network SR of skeletal myofibers and is thus the first example of a sequence that targets proteins to a particular compartment of the SR...
  100. pmc The vanilloid receptor TRPV1 is activated and sensitized by local anesthetics in rodent sensory neurons
    Andreas Leffler
    Department of Anesthesiology, Friedrich Alexander University Erlangen Nuremberg, Erlangen, Germany
    J Clin Invest 118:763-76. 2008
    ..This effect is sufficient to release CGRP, a key component of neurogenic inflammation, and warrants investigation into the role of TRPV1 and TRPA1 in LA-induced neurotoxicity...
  101. ncbi Structure and organization of the human ankyrin-1 gene. Basis for complexity of pre-mRNA processing
    P G Gallagher
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 272:19220-8. 1997
    ..Utilization of alternative polyadenylation signals was found to be the basis for the previously described, stage-specific 9.0- and 7.2-kilobase pair transcripts of the ANK-1 gene...

Research Grants65

  1. Roles of Nitric Oxide and Superoxide in Cystitis
    ANTHONY JOHN KANAI; Fiscal Year: 2013
    ..Hypothesis: FA-NO2 are radioprotective by desensitizing TRPA1 and TRPV1 channels and BDNF-AB and NGF-AB are therapeutic by inhibiting nerve growth and sensitization in bladder and spinal cord. ..
  2. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Quentin J Baca; Fiscal Year: 2010
    ..The applicant is an excellent candidate for this award and has outstanding potential to combine basic science with clinical applications as an MD-PhD. ..
  3. Role of spectrin/ankyrin-G complex in myocyte signaling and cardiac excitability
    Peter J Mohler; Fiscal Year: 2013
    ..abstract_text> ..
  4. Distinct roles of CARP target genes in wound healing
    Karinna Almodóvar; Fiscal Year: 2013
    ..Results derived from this study can lead to the delineation of new mechanisms that regulate wound angiogenesis. ..
  5. Recombinant Antibodies from the Sea Lamprey
    ZEEV contact PANCER; Fiscal Year: 2010
    ..We propose to develop high affinity recombinant antibodies from the sea lamprey as a completely new class of recognition molecules for diagnostic, analytic, and biosensor applications in biotechnology. ..
  6. MURINE TYPHUS: VECTOR BIOLOGY AND TRANSMISSION
    Abdu F Azad; Fiscal Year: 2013
    ..typhi we hypothesize that disrupting the molecular balance between R. typhi and the flea vector should affect flea fitness during infection. ..
  7. The influence of genotype on the outcome of gene transfer in beta-thalassemia
    Stefano Rivella; Fiscal Year: 2013
    ..With this model, based on the genotype and analysis of the mRNA stability, we aim to predict the potential success of gene therapy given a specific mutation. ..
  8. Orientia tsutsugamushi modulation of host cell ubiquitination machinery
    Jason A Carlyon; Fiscal Year: 2013
    ..In Aim 2, we will close an important knowledge gap by identifying the Anks that the pathogen expresses as it establishes infection in each of the 3 biologically relevant host cells. ..
  9. Subverted host cell signaling by AnkA in Anaplasma phagocytophilum infection
    Jacob W IJdo; Fiscal Year: 2012
    ..phagocytophilum can survive in neutrophils. This may lead to a better understanding of the function of neutrophils within the immune system and may lead to potential new treatments of inflammation and infections. ..
  10. Mechanisms of cardiac Ca dysregulation and arrhythmias in ankyrin B deficiency
    Sanda Despa; Fiscal Year: 2013
    ....
  11. Asb2 in CD4+ T cell lineage differentiation and its plasticity
    Xiao Hong Sun; Fiscal Year: 2013
    ..Fresh ideas will likely emerge from these studies that will be o therapeutic value in the contest of immunity and autoimmunity. ..
  12. Role of ankyrin-B in human arrhythmia
    Peter J Mohler; Fiscal Year: 2013
    ..For this first competitive renewal, we present a cast of uncharacterized and innovative animal models, novel molecular tools, innovative technologies, and new antibodies to test the specific roles of the AnkB cellular pathway in vivo. ..
  13. Host protein prenyl transferases as modifiers of effectors of pathogenic bacteria
    ELIA WRIGHT; Fiscal Year: 2013
    ..Knowledge of how effecto proteins are modified by eukaryotic host cells may lead to the identification of novel pathways that could be exploited for the development of antibacterials. ..
  14. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2010
    ..This knowledge will advance and strengthen understanding of the key molecules that protect the kidney from injury and enhance recovery. ..
  15. FUNCTIONAL ANALYSES OF NEUROGLIAN/L1 IN SYNAPTOGENESIS
    TANJA ANGELA GODENSCHWEGE; Fiscal Year: 2012
    ..This information is essential to find appropriate treatment for the clinical manifestations of these mutations and hence will benefit the health of patients with L1-related disorders in the future. ..
  16. Structural and Signaling Roles for Cardiac Ankyrin-B
    SHANE CUNHA; Fiscal Year: 2013
    ..Similar findings of tissue-specific alternative isoforms for the related ankyrins (-G and -R) provide support for our central hypothesis that the human heart maintains a previously unidentified ..
  17. Defining the Mammalian Intercellular Bridge Interactome
    Martin M Matzuk; Fiscal Year: 2010
    ..Our proposed Specific Aims will also help us to develop additional high throughput screening assays for the identification of small molecule disruptors of the intercellular bridge. ..
  18. The Role of Small Ankyrin 1 in the Sarcoplasmic Reticulum in Skeletal Muscle
    Andrew P Ziman; Fiscal Year: 2011
    ..My studies should provide insights into the mechanisms underlying the organization of the calcium stores, changes in which have been implicated in a range of diseases in heart and skeletal muscle. ..
  19. Notch-induced protein degradation in lymphopoiesis
    Xiao Hong Sun; Fiscal Year: 2013
    ..There is good reason to believe the answers to these questions will lead to new treatments for several types of diseases. These include leukemias and cancers of the breast or intestine, autoimmune diseases and developmental defects. ..
  20. Fiber Cell Cytoskeletal Scaffolds-Role in Lens Architecture and Function
    P Vasantha Rao; Fiscal Year: 2011
    ....
  21. Nutritional Copper Signaling and Homeostasis
    Sabeeha Merchant; Fiscal Year: 2010
    ..Cu cells and err I vs. wild-type cells, in parallel with sub-proteomic gel- and multi-dimensional chromatography-based analyses of copper-replete vs. copper-deficient cells. ..
  22. Obesity is a Surgical Problem
    Michael W Mulholland; Fiscal Year: 2013
    ..We hypothesize that directed overexpression of Asb-4 in hypothalamic proopiomelanocortin neurons will decrease food intake, increase energy expenditure and confer resistance to high fat diet-induced obesity. ..
  23. Structural studies of a spectrin/ankyrin complex
    ALFONSO MONDRAGON; Fiscal Year: 2013
    ..Defects in these proteins are linked to two blood disorders, hereditary spherocytosis and hereditary elliptocytosis. In addition, ankyrin defects are involved in long-QT cardiac arrhythmia and other cardiac problems. ..
  24. Functional characterization of Orientia tsutsugamushi ankryin repeat proteins
    Jason A Carlyon; Fiscal Year: 2013
    ..tsutsugamushi isolates is increasing and reinfections are common, defining the functional roles of Anks will potentially aid the design of small molecule inhibitors that target specific Anks to treat scrub typhus. ..
  25. CaMKII-dependent regulation of cardiac excitability
    THOMAS JEFFREY HUND; Fiscal Year: 2013
    ..5 and cell excitability, and identify novel mechanisms for arrhythmias in both congenital and acquired heart disease. ..
  26. Assembly of the Dystrophin-Glycoprotein Complex
    Jeffrey S Chamberlain; Fiscal Year: 2013
    ..determinants of dystrophin (and its paralog utrophin) that mediate interactions with [unreadable]-actin, the ankyrins, [unreadable]-dystrobrevins, [unreadable]-dystroglycan, the sarcoglycans and nitric oxide synthase...
  27. The Role of Ankyrin-B Mutations in Premature Senescence
    KENT RONALD NILSSON; Fiscal Year: 2012
    ....
  28. Repeat-Proteins;Stability, Folding Kinetics &Evolution
    DOUGLAS E BARRICK; Fiscal Year: 2012
    ..These rules will help us to understand the causes of "folding diseases", and will also provide new biomaterials that can be used to diagnose and perhaps ultimately treat human diseases. ..
  29. Molecular basis of ILK-mediated cell adhesion
    Jun Qin; Fiscal Year: 2013
    ..They may also impact on our understanding of multiple ILK-associated diseases and also re-invigorate strategies for the ILK-targeted therapy. ..
  30. KATP channel expression and localization in the progression of heart failure
    DENICE HODGSON-ZINGMAN; Fiscal Year: 2012
    ..abstract_text> ..
  31. Mechanisms of Node of Ranvier Assembly
    James Salzer; Fiscal Year: 2013
    ....
  32. Cellular Physiology of Sensory Ion Channels
    David Julius; Fiscal Year: 2013
    ..Together, these studies will provide a rational basis for the development and design of novel analgesic agents for controlling chronic pain and neurogenic inflammatory syndromes. ..
  33. Farnesylated effectors of Legionella
    YOUSEF A ABU KWAIK; Fiscal Year: 2013
    ....
  34. Filamin interactions in differentiation, invasion and disease
    David A Calderwood; Fiscal Year: 2013
    ....
  35. FUNCTIONAL ANALYSIS OF DISTINCT NOCICEPTOR POPULATIONS
    CHERYL LOUISE STUCKY; Fiscal Year: 2013
    ..Our goals are to determine the roles of the ion channel Transient Receptor Potential Ankyrin 1 (TRPA1) in touch and cold transduction in normal skin, and to identify its roles in neuropathic and inflammatory pain models. ..
  36. Technology Development for Recombinant Affinity Reagents
    BRIAN KENNETH KAY; Fiscal Year: 2013
    ..The overarching goal of our research will be to optimize technologies that can be applied to the entire human proteome in a versatile, powerful, cost-effective, and high-throughput manner. ..
  37. Prediction and Design of Nucleic Acid Recognition by Repeat Proteins
    Philip Bradley; Fiscal Year: 2013
    ....
  38. Protein targeting in vertebrate photoreceptors
    Sheila A Baker; Fiscal Year: 2013
    ..the selective interaction of Na/K-ATPase, a known ankyrin binding protein, with ankyrin-B but not other ankyrins is a feature of the specific alpha subunit of Na/K-ATPase expressed in photoreceptors and that this selective ..
  39. Molecular and Cellular Mechanisms of Synaptic Growth
    Graeme W Davis; Fiscal Year: 2012
    ..We propose to define the function of these genes at the neuromuscular junction, thereby significantly advancing our understanding of the genetic factors that may contribute to neuromuscular degenerative disease. ..
  40. Mitochondrial reactive oxygen species induce airway sensory nerve activity
    THOMAS EDWARD TAYLOR-CLARK; Fiscal Year: 2013
    ....
  41. Enzyme-delivery scaffold technology for targeted cancer killing.
    Arnon Lavie; Fiscal Year: 2013
    ..coli. Moreover, every aspect of the delivery scaffold, from binding affinity via internalization propensity, to escape from vesicles into the cytoplasm will be optimized by coupling phage display with the appropriate selection method. ..
  42. Genomics-Guided Characterization of iPS Cells from Common Mental Illnesses
    STEPHEN JOHN HAGGARTY; Fiscal Year: 2010
    ..Methods for inducing the differentiation of iPS cells into neural and glial cells will be implemented as well as novel methods for phenotyping cells using imaging and plate reader assays. ..
  43. Genomics-Guided Characterization of iPS Cells from Common Mental Illnesses
    Stephen J Haggarty; Fiscal Year: 2012
    ..Methods for inducing the differentiation of iPS cells into neural and glial cells will be implemented as well as novel methods for phenotyping cells using imaging and plate reader assays. ..
  44. Inherited Dysfunction of Stretch Based Signaling in Dilated Cardiomyopathy
    Mousumi Moulik; Fiscal Year: 2012
    ..The institutional environment, available resources and the mentoring plan are ideal for meeting the goals of this career development proposal and will establish the PI as an independent physician-scientist. (End of Abstract) ..
  45. Enhancing mammalian glycoprotein production in the baculovirus expression vector
    Christoph Geisler; Fiscal Year: 2013
    ..This new technology should be relatively easy to commercialize based on the established reputations of ParaTechs, Inc. and GlycoBac, LLC and the growing demand for improved cell lines to express recombinant humanized glycoproteins. ..
  46. Obscurin & Myofibrils in Cardiac & Skeletal Muscle
    Robert Bloch; Fiscal Year: 2009
    ..The results of our experiments should reveal some of the key mechanisms involved in assembling contractile proteins into sarcomeres and in determining their association with the SR. ..
  47. Mechanism and function of Kv channel targeting
    Chen Gu; Fiscal Year: 2013
    ..Therefore, our project to understand how Kv channels are localized to regulate functions of nerve cells will contribute to generate novel strategies for treating diseases of the nervous systems. ..
  48. Enhancing mammalian glycoprotein production in the baculovirus expression vector
    Donald L Jarvis; Fiscal Year: 2010
    ....
  49. Organization of Sarcoplasmic Reticulum in Skeletal Muscle
    Robert J Bloch; Fiscal Year: 2012
    ..Defects in these structures have been linked to myopathies and muscular dystrophies, but how they function in muscle is still poorly understood. This proposal is to study the structures in skeletal muscle that store calcium ions. ..
  50. FGF14 IN THE REGULATION OF PURKINJE NEURON EXCITABILITY AND SCA27
    David M Ornitz; Fiscal Year: 2013
    ....
  51. Circuit-specific Bidirectional Transcript Modulation of Psychiatric Risk Genes
    Tracey Petryshen; Fiscal Year: 2013
    ..While this proposal focuses on ANK3, it will also serve as a proof-of-concept study to establish novel isoform-specific TALE technology as a general strategy that will have broad applications across molecular neuroscience. ..
  52. Neuroglial Interactions at the Node of Ranvier
    Matthew N Rasband; Fiscal Year: 2013
    ..Among these, the axonal submembranous cytoskeleton comprised of ankyrins and spectrins has been proposed to be key components...
  53. ClipR-59: a novel regulator of Akt signaling
    Keyong Du; Fiscal Year: 2009
    ..Overall, the studies proposed here will demonstrate that ClipR-59 is a novel regulator of Akt signaling and may provide insight knowledge regarding how Akt signaling is specified. ..
  54. TRPV4: Mechanosensitivity and Skeletal Dysplasia
    Ching Kung; Fiscal Year: 2013
    ..This work aims towards understanding how ARD mutations can lead to two disparate diseases. In collaboration, we will also use trpV4-/- knock-out mice to test the role of TRPV4 in fracture healing. ..
  55. UNDERSTANDING THE INTERACTION OF ANKYRINS AND NANOMATERIALS
    CARLOS DIEGO GARCIA; Fiscal Year: 2013
    ..For these studies, we have selected a group of proteins called ankyrins. These proteins display an unusually high stability and fully reversible spring-like behavior...
  56. Role of Desmosomes in Cardiac Electrical Function
    Mario Delmar; Fiscal Year: 2013
    ..abstract_text> ..
  57. Ependymal control of new neuron production in the adult brain
    Chay Titus Kuo; Fiscal Year: 2013
    ....
  58. Vaccinia Proteome Affinity Reagents From Phage Display
    Philip Felgner; Fiscal Year: 2005
    ..Successful "proof of concept" demonstrations in any of these assays will form the basis for a Phase II application leading, to arrays of analytical proteomics reagents against the other Class A, B & C bioterrorism agents. ..
  59. De novo engineering of protein agonists and antagonists
    CASIM SARKAR; Fiscal Year: 2013
    ..More generally, these methods will not only enable the production of new therapeutics, but will also provide unique insights into the basic requirements for specific, high-affinity protein recognition. ..
  60. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2006
    ..Together, the proposed studies on FP-2 may begin to reveal molecular mechanisms underlying the release of malaria parasite from the host erythrocyte. ..
  61. Molecular Basis of Pathogen-Induced Cell Death in Plants
    JEAN GREENBERG; Fiscal Year: 2009
    ..Because experimental progress using a plant model is rapid, the results obtained and quickly be related to human biology and disease. ..
  62. ARCHITECTURE OF ERYTHROCYTE SKELETON--BILAYER INTERFACE
    STEPHEN SHOHET; Fiscal Year: 2000
    ..In aggregate, the role of bilayer-skeletal interactions in red cell membrane structure and stability will be more clearly defined. ..
  63. MOLECULAR BIOLOGY OF HUMAN ERYTHROCYTE ANKYRIN
    Patrick Gallagher; Fiscal Year: 2000
    ..These studies will provide important insights into our understanding of the role of ankyrin in normal and disease states. ..
  64. NR/CAM AND FORMATION OF NODE OF RANVIER
    Martin Grumet; Fiscal Year: 2002
    ..In the future, such information may lead to new therapeutic strategies to prevent injury and restore function in myelinated nerves that become damaged and remyelinated in multiple sclerosis. ..
  65. BIOGENESIS OF THE RED CELL MEMBRANE AND CYTOSKELETON
    Hagop Youssoufian; Fiscal Year: 1993
    ..In addition to probing basic mechanisms of protein sorting, these studies should improve our understanding of hemolytic anemias by providing cell culture models for these disorders...