beta crystallin b chain

Summary

Summary: The basic subunit of beta-crystallins.

Top Publications

  1. pmc Deamidation destabilizes and triggers aggregation of a lens protein, betaA3-crystallin
    Takumi Takata
    Department of Integrative Biosciences, School of Dentistry, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    Protein Sci 17:1565-75. 2008
  2. ncbi Elongation of axons during regeneration involves retinal crystallin beta b2 (crybb2)
    Thomas Liedtke
    Department of Experimental Ophthalmology, University of Munster, Germany
    Mol Cell Proteomics 6:895-907. 2007
  3. pmc Dual roles for Prox1 in the regulation of the chicken betaB1-crystallin promoter
    Xiaoren Chen
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
    Invest Ophthalmol Vis Sci 49:1542-52. 2008
  4. ncbi A missense mutation S228P in the CRYBB1 gene causes autosomal dominant congenital cataract
    Jun Wang
    Beijing Tongren Eye Center, Capital Medical University, Beijing 100730, China
    Chin Med J (Engl) 120:820-4. 2007
  5. pmc Crystal structure of truncated human betaB1-crystallin
    Rob L M Van Montfort
    Department of Crystallography, Birkbeck College, London WC1E 7HX, UK
    Protein Sci 12:2606-12. 2003
  6. ncbi Decreased heat stability and increased chaperone requirement of modified human betaB1-crystallins
    Kirsten J Lampi
    Department of Oral Molecular Biology, Oregon Health and Science University, Portland, OR, USA
    Mol Vis 8:359-66. 2002
  7. ncbi Aey2, a new mutation in the betaB2-crystallin-encoding gene of the mouse
    J Graw
    Forschungszentrum für Umwelt und Gesundheit GSF Institute of Experimental Genetics, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 42:1574-80. 2001
  8. pmc A novel nonsense mutation in CRYBB1 associated with autosomal dominant congenital cataract
    Juhua Yang
    Biomedical Engineering Center of Fujian Medical University, Fuzhou, Fujian, China
    Mol Vis 14:727-31. 2008
  9. pmc A nonsense mutation in CRYBB1 associated with autosomal dominant cataract linked to human chromosome 22q
    Donna S Mackay
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    Am J Hum Genet 71:1216-21. 2002
  10. ncbi Human beta-crystallins modified by backbone cleavage, deamidation and oxidation are prone to associate
    Zhongli Zhang
    Department of Chemistry, University of Nebraska, Lincoln, NE 68588 0304, USA
    Exp Eye Res 77:259-72. 2003

Research Grants

  1. BIOMARKERS FOR TOTAL BODY BURDEN OF FLUORIDE
    Kirsten Lampi; Fiscal Year: 2004
  2. MECHANISMS OF CHAPERONE FUNCTION IN THE LENS
    Hassane McHaourab; Fiscal Year: 2008
  3. AUTOSOMAL DOMINANT CATARACTS
    J Bateman; Fiscal Year: 2004

Scientific Experts

  • Veronica Harrington
  • KIRSTEN JEANNE LAMPI
  • J Fielding Hejtmancik
  • L Regan
  • Ke Yao
  • Hassane McHaourab
  • Y V Sergeev
  • J Graw
  • Ying Wang
  • J Bronwyn Bateman
  • Takumi Takata
  • Bing Fen Liu
  • Melinda K Duncan
  • Jennifer R Taube
  • Xiaoren Chen
  • Noah E Robinson
  • Larry L David
  • Jack J N Liang
  • M Satish Kumar
  • Hanane A Koteiche
  • Xu Ma
  • K Srivastava
  • Christine Slingsby
  • Phillip A Wilmarth
  • O P Srivastava
  • Nobuhiko Mizuno
  • Yoko Ueda
  • Nicolette H Lubsen
  • Magalie Michiel
  • Jason A Lampi
  • Edward J Dudek
  • Gwen M Sturgill
  • Q C An
  • Sindhu Saraswathy
  • Esther Meyer
  • Divya Kapoor
  • Monika B Dolinska
  • Maneo Emily Mothobi
  • Lars Hansen
  • Sandor Boros
  • Koustav Ganguly
  • Jie Zhang
  • O A Bateman
  • Tapan P Patel
  • J Keenan
  • Vladimir I Simirskii
  • Jun Zhang
  • Ling Chen
  • Paul Evans
  • C Muller
  • Juhua Yang
  • Fei Feng Li
  • Si Quan Zhu
  • Feng Gu
  • Thomas Liedtke
  • Silke Pauli
  • Jun Wang
  • David Cohen
  • Kevin M Duprey
  • Maroor K Jobby
  • A V Krivandin
  • Laura Marin-Vinader
  • Rebecca J Kapphahn
  • Hsin Han Hou
  • Markus Kroeber
  • Lynn K Gordon
  • Gary Kruppa
  • Arthur B Robinson
  • E G Padmanabha Udupa
  • Padmanabha E G Udupa
  • Yogendra Sharma
  • Onofrio Annunziata
  • S Amer Riazuddin
  • Colin E Willoughby
  • Hisato Kondoh
  • Sathiyavedu T Santhiya
  • Michael J Harms
  • K Krishna Sharma
  • David Stempel
  • Gabriela Viteri
  • M K Jobby
  • Jun tao Guo
  • Zhongli Zhang
  • Rob L M Van Montfort
  • Latifa Hilal
  • R Senthilkumar
  • Donna S Mackay
  • Ling Fu
  • C Slingsby
  • N J Clout

Detail Information

Publications84

  1. pmc Deamidation destabilizes and triggers aggregation of a lens protein, betaA3-crystallin
    Takumi Takata
    Department of Integrative Biosciences, School of Dentistry, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    Protein Sci 17:1565-75. 2008
    ..A potential mechanism may include decreased stability and/or altered interactions with other beta-subunits. Understanding the role of deamidation in the long-lived crystallins has important implications in other aggregation diseases...
  2. ncbi Elongation of axons during regeneration involves retinal crystallin beta b2 (crybb2)
    Thomas Liedtke
    Department of Experimental Ophthalmology, University of Munster, Germany
    Mol Cell Proteomics 6:895-907. 2007
    ..The results suggest that neuronal crystallins constitute a novel class of neurite-promoting factors that likely operate through an autocrine mechanism and that they could be used in neurodegenerative diseases...
  3. pmc Dual roles for Prox1 in the regulation of the chicken betaB1-crystallin promoter
    Xiaoren Chen
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
    Invest Ophthalmol Vis Sci 49:1542-52. 2008
    ....
  4. ncbi A missense mutation S228P in the CRYBB1 gene causes autosomal dominant congenital cataract
    Jun Wang
    Beijing Tongren Eye Center, Capital Medical University, Beijing 100730, China
    Chin Med J (Engl) 120:820-4. 2007
    ..Congenital cataract is a highly heterogeneous disorder at both the genetic and phenotypic levels. This study was conducted to identify disease locus for autosomal dominant congenital cataracts in a four generation Chinese family...
  5. pmc Crystal structure of truncated human betaB1-crystallin
    Rob L M Van Montfort
    Department of Crystallography, Birkbeck College, London WC1E 7HX, UK
    Protein Sci 12:2606-12. 2003
    ..The crystal structure shows that the truncated betaB1 dimer is extremely well suited to form higher-order lattice interactions using its hydrophobic surface patches, linker regions, and sequence extensions...
  6. ncbi Decreased heat stability and increased chaperone requirement of modified human betaB1-crystallins
    Kirsten J Lampi
    Department of Oral Molecular Biology, Oregon Health and Science University, Portland, OR, USA
    Mol Vis 8:359-66. 2002
    ..To determine how deamidation and partial loss of the N- and C-terminal extensions alter the heat stability of betaB1-crystallin...
  7. ncbi Aey2, a new mutation in the betaB2-crystallin-encoding gene of the mouse
    J Graw
    Forschungszentrum für Umwelt und Gesundheit GSF Institute of Experimental Genetics, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 42:1574-80. 2001
    ..One particular mutant was found that caused progressive opacity and was referred to as Aey2. The purpose of the study was to provide a morphologic description, to map the mutant gene, and to characterize the underlying molecular lesion...
  8. pmc A novel nonsense mutation in CRYBB1 associated with autosomal dominant congenital cataract
    Juhua Yang
    Biomedical Engineering Center of Fujian Medical University, Fuzhou, Fujian, China
    Mol Vis 14:727-31. 2008
    ..To identify the molecular defect underlying an autosomal dominant congenital nuclear cataract in a Chinese family...
  9. pmc A nonsense mutation in CRYBB1 associated with autosomal dominant cataract linked to human chromosome 22q
    Donna S Mackay
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    Am J Hum Genet 71:1216-21. 2002
    ..This study has identified the first CRYBB1 mutation associated with autosomal dominant cataract in humans...
  10. ncbi Human beta-crystallins modified by backbone cleavage, deamidation and oxidation are prone to associate
    Zhongli Zhang
    Department of Chemistry, University of Nebraska, Lincoln, NE 68588 0304, USA
    Exp Eye Res 77:259-72. 2003
    ..The greater solubility of beta-crystallins compared with alpha- and gamma-crystallins in aging lenses may be due to beta-crystallin modifications and their non-covalent associations...
  11. ncbi Domain interaction sites of human lens betaB2-crystallin
    Bing Fen Liu
    Center for Ophthalmic Research Surgery, Brigham and Women s Hospital, and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:2624-30. 2006
    ..The V60E and V144E mutants were found to be partially unfolded and incapable of forming a complete dimer...
  12. ncbi Progressive polymorphic congenital cataract caused by a CRYBB2 mutation in a Chinese family
    Ke Yao
    Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou, China
    Mol Vis 11:758-63. 2005
    ..To report and identify the genetic defect that causes progressive polymorphic congenital cataracts affecting a large five generation Chinese family...
  13. ncbi CRYBB1 mutation associated with congenital cataract and microcornea
    Colin E Willoughby
    Department of Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, Canada
    Mol Vis 11:587-93. 2005
    ..The molecular characterization of a UK family with an autosomal dominant congenital cataract associated with microcornea is reported...
  14. pmc Laser light-scattering evidence for an altered association of beta B1-crystallin deamidated in the connecting peptide
    Michael J Harms
    Oregon Health and Science University, Portland, OR 97239, USA
    Protein Sci 13:678-86. 2004
    ..Deamidation at Gln146, but not Asn157, led to an increased tendency of beta B1 to aggregate upon heating. We conclude that deamidation creates unique effects depending upon where the deamidation is introduced in the crystallin structure...
  15. ncbi Mutations in betaB3-crystallin associated with autosomal recessive cataract in two Pakistani families
    S Amer Riazuddin
    Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Invest Ophthalmol Vis Sci 46:2100-6. 2005
    ..To identify the disease locus for autosomal recessive congenital cataracts in consanguineous Pakistani families...
  16. ncbi Sequence analysis of betaA3, betaB3, and betaA4 crystallins completes the identification of the major proteins in young human lens
    K J Lampi
    Department of Oral Molecular Biology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Biol Chem 272:2268-75. 1997
    ..These studies were a prerequisite for future studies to determine how human lens proteins are altered during aging and cataract formation...
  17. pmc Deamidation in human lens betaB2-crystallin destabilizes the dimer
    Kirsten J Lampi
    Department of Integrative Biosciences, School of Dentistry, Oregon Health and Science University, 611 SW Campus Drive, Portland, Oregon 97239 3098, USA
    Biochemistry 45:3146-53. 2006
    ..These results suggest that deamidation at critical sites destabilizes betaB2 and may disrupt the function of betaB2 in the lens...
  18. ncbi Mutation analysis of congenital cataracts in Indian families: identification of SNPS and a new causative allele in CRYBB2 gene
    Sathiyavedu T Santhiya
    Dr ALM Postgraduate Institute of Basic Medical Sciences, Department of Genetics, University of Madras, Taramani, Chennai, India
    Invest Ophthalmol Vis Sci 45:3599-607. 2004
    ..To study some functional candidate genes in cataract families of Indian descent...
  19. ncbi Homozygous CRYBB1 deletion mutation underlies autosomal recessive congenital cataract
    David Cohen
    The Morris Kahn Laboratory of Human Genetics, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Invest Ophthalmol Vis Sci 48:2208-13. 2007
    ..Two extended unrelated consanguineous inbred Bedouin families from southern Israel presenting with autosomal recessive congenital nuclear cataract were studied...
  20. ncbi Genetic heterogeneity of the Coppock-like cataract: a mutation in CRYBB2 on chromosome 22q11.2
    D Gill
    Eye Research Institute of Canada, Toronto, Ontario
    Invest Ophthalmol Vis Sci 41:159-65. 2000
    ..To identify the genetic defect for the Coppock-like cataract (CCL) affecting a Swiss family, which defect was unlinked to the chromosome 2q33-35 CCL locus...
  21. ncbi Gene conversion mutation in crystallin, beta-B2 (CRYBB2) in a Chilean family with autosomal dominant cataract
    J Bronwyn Bateman
    Department of Ophthalmology, Rocky Mountain Lions Eye Institute, Denver, Colorado, USA
    Ophthalmology 114:425-32. 2007
    ..To map and identify the mutated gene for autosomal dominant cataract (ADC) in a large Chilean family (ADC53)...
  22. pmc Subfertility in mice harboring a mutation in betaB2-crystallin
    Kevin M Duprey
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Mol Vis 13:366-73. 2007
    ..Since we found mice harboring the Crybb2Phil mutation bred poorly, here we assess the contribution of betaB2-crystallin to mouse fertility and determine the expression pattern of betaB2-crystallin in the testis...
  23. ncbi Quantitative measurement of young human eye lens crystallins by direct injection Fourier transform ion cyclotron resonance mass spectrometry
    Noah E Robinson
    Oregon Institute of Science and Medicine, Cave Junction, OR 97523, USA
    Mol Vis 12:704-11. 2006
    ..We report herein the measurement of the 14 crystallins in 10 pairs of lenses from humans between the ages of 2 and 300 days...
  24. pmc Expression of betaB(2)-crystallin mRNA and protein in retina, brain, and testis
    K S Magabo
    Department of Biology, West Virginia University, Morgantown 26505, USA
    Invest Ophthalmol Vis Sci 41:3056-60. 2000
    ..To evaluate the expression of betaB(2)-crystallin mRNA and protein in rat, bovine, and human nonlens and nonocular tissues...
  25. pmc Mutation analysis in a German family identified a new cataract-causing allele in the CRYBB2 gene
    Silke Pauli
    Institute of Human Genetics, University of Gottingen, Gottingen, Germany
    Mol Vis 13:962-7. 2007
    ..The study demonstrates the functional candidate gene analysis in a cataract family of German descent...
  26. ncbi Beta-crystallin association
    J F Hejtmancik
    National Eye Institute NIH, Ophthalmic Genetics and Visual Function Branch, Building 10, Room 10B10, 10 Center Drive MSC 1860, Bethesda, MD 20892, USA
    Exp Eye Res 79:377-83. 2004
    ..As predicted by molecular modelling, the fraction of beta-Crystallin present as dimers increases with increasing temperature, implying that beta-crystallin association is entropically driven...
  27. ncbi Interaction and biophysical properties of human lens Q155* betaB2-crystallin mutant
    Bing Fen Liu
    Center for Ophthalmic Research Surgery, Brigham and Women s Hospital, Boston, MA 02115, USA
    Mol Vis 11:321-7. 2005
    ..We sought to determine the changes in conformation and protein-protein interactions brought about by this mutation...
  28. ncbi Crystallins in water soluble-high molecular weight protein fractions and water insoluble protein fractions in aging and cataractous human lenses
    Veronica Harrington
    Department of Physiological Optics, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Mol Vis 10:476-89. 2004
    ..Because these changes were cataract specific and not aging specific, the results were expected to provide information regarding potential mechanisms of age related cataract development...
  29. ncbi Novel allele of crybb2 in the mouse and its expression in the brain
    Koustav Ganguly
    Institutes of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany
    Invest Ophthalmol Vis Sci 49:1533-41. 2008
    ..O377 was identified as a new dominant cataract mutation in mice after radiation experiments. The purpose of this study was to genetically characterize the mutation and to analyze its biological consequences...
  30. ncbi Evidence of clinical and genetic heterogeneity in autosomal dominant congenital cerulean cataracts
    Latifa Hilal
    Laboratoire de Génétique et Biologie Moléculaire de la Faculté des Sciences, Université Ibn Tofail, Kenitra, Morocco
    Ophthalmic Genet 23:199-208. 2002
    ..This finding confirms the clinical and genetic heterogeneity of autosomal dominant congenital cerulean cataracts...
  31. pmc PCNA interacts with Prox1 and represses its transcriptional activity
    Xiaoren Chen
    Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
    Mol Vis 14:2076-86. 2008
    ..This paper seeks to better understand the role of protein-protein interactions in this multitude of functions...
  32. pmc Patterns of crystallin distribution in porcine eye lenses
    J Keenan
    School of Biomedical Sciences, University of Ulster, Coleraine, UK
    Mol Vis 14:1245-53. 2008
    ..To measure the protein distribution patterns in single young porcine lenses...
  33. ncbi Protein redesign
    L Regan
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, New Haven, CT 06520, USA
    Curr Opin Struct Biol 9:494-9. 1999
    ....
  34. pmc Phase behavior of mixtures of human lens proteins Gamma D and Beta B1
    Ying Wang
    Materials Processing Center, Department of Physics, and Center for Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:13282-7. 2010
    ..Our findings provide insight into the factors essential for maintaining lens proteins in a single homogeneous phase, thereby enabling lens transparency...
  35. ncbi BetaB1-crystallin: identification of a candidate ciliary body uveitis antigen
    David Stempel
    Department of Ophthalmology, University of California Los Angeles, Jules Stein Eye Institute, 100 Stein Plaza, Los Angeles, CA 90095, USA
    Invest Ophthalmol Vis Sci 44:203-9. 2003
    ..In this study, a recombinantly isolated pANCA monoclonal antibody was used to identify the corresponding antigenic target(s) in the ciliary body...
  36. ncbi Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1
    Q C An
    Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China
    Mol Biol Rep 36:683-90. 2009
    ..Our experiment is the first to establish the primary foundation for further research on these three swine genes...
  37. ncbi Retinal proteins modified by 4-hydroxynonenal: identification of molecular targets
    Rebecca J Kapphahn
    Department of Ophthalmology, University of Minnesota, 380 Lions Research Building, 2001 6th Street SE, Minneapolis, MN 55455, USA
    Exp Eye Res 83:165-75. 2006
    ..These results identify a common subset of proteins that contain HNE adducts and suggest that select retinal proteins are molecular targets for HNE modification...
  38. ncbi Transgenic studies on the role of optineurin in the mouse eye
    Markus Kroeber
    Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Exp Eye Res 82:1075-85. 2006
    ..Our results show that optineurin is a cytoplasmatic rather than a secretory protein that is preferentially expressed in retinal ganglion cells, and argue against a major role of optineurin for the modulation of apoptosis in vivo...
  39. pmc Mutation analysis of congenital cataract in a Basotho family identified a new missense allele in CRYBB2
    Maneo Emily Mothobi
    Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, China
    Mol Vis 15:1470-5. 2009
    ..To identify the causative genetic mutation among the known cataract candidate genes underlying the observed phenotype in a Basotho family, with congenital nuclear cataracts...
  40. pmc Deamidation alters interactions of beta-crystallins in hetero-oligomers
    Takumi Takata
    Department of Integrative Biosciences, School of Dentistry, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Mol Vis 15:241-9. 2009
    ..The purpose of the present study was to investigate if deamidation altered the interaction of betaA3 with other beta-crystallin subunits...
  41. ncbi Mutation screen of beta-crystallin genes in 274 patients with age-related macular degeneration
    Gwen M Sturgill
    Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA
    Ophthalmic Genet 31:129-34. 2010
    ..We therefore investigated CRYBB1 and CRYBB2 as candidate genes for AMD in 274 unrelated patients...
  42. ncbi The stability of human acidic beta-crystallin oligomers and hetero-oligomers
    O A Bateman
    Department of Crystallography, Birkbeck College, London, UK
    Exp Eye Res 77:409-22. 2003
    ..Circular dichroism and fluorescence spectroscopies show that homo-dimer surface tryptophans become buried in the betaA3/1/betaB1-crystallin hetero-dimer concomitant with changes in polypeptide chain conformation...
  43. ncbi Tissue transglutaminase catalyzes the deamidation of glutamines in lens betaB(2)- and betaB(3)-crystallins
    Sandor Boros
    Department of Biomolecular Chemistry 271, Nijmegen Center for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands
    Exp Eye Res 86:383-93. 2008
    ..These data together suggest that tTG can contribute to the age-related deamidation of glutamine residues of lens crystallins...
  44. ncbi Linkage and association analyses of intragenic SNPs in the canine beta-crystallin genes CRYBB1, CRYBB2, CRYBB3, CRYBA1 and CRYBA4 with primary cataracts in wire-haired Dachshunds
    C Muller
    Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bunteweg 17p, 30559 Hannover, Germany
    Anim Genet 39:87-8. 2008
  45. pmc Aggregation of deamidated human betaB2-crystallin and incomplete rescue by alpha-crystallin chaperone
    Magalie Michiel
    Protéines, Biochimie Structurale et Fonctionnelle, CNRS UPMC, Case 29, 7 quai St Bernard, 75252 Paris Cedex 5, France
    Exp Eye Res 90:688-98. 2010
    ..A potential mechanism for cataract formation in vivo involving accumulation of deamidated beta-crystallin aggregates is discussed...
  46. pmc N-terminal extension of beta B1-crystallin: identification of a critical region that modulates protein interaction with beta A3-crystallin
    Monika B Dolinska
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 48:9684-95. 2009
    ..The loop is derived from residues 48-56 which appear to be critical for mediating protein interactions with betaA3-crystallin...
  47. ncbi Identification and properties of anti-chaperone-like peptides derived from oxidized bovine lens betaL-crystallins
    R Senthilkumar
    Mason Eye Institute and the Department of Biochemistry, University of Missouri, Columbia, Missouri 65212, USA
    J Biol Chem 277:39136-43. 2002
    ..These data suggest that the polypeptides generated after oxidation and proteolysis of betaL-crystallins interact with denaturing proteins and facilitate their aggregation and light scattering, thus behaving like anti-chaperones...
  48. ncbi Identification of the primary targets of carbamylation in bovine lens proteins by mass spectrometry
    Jie Zhang
    Department of Ophthalmology, Tangdu Hospital, Fourth Military Medical University, Xi an, China
    Curr Eye Res 33:963-76. 2008
    ..The aim of this study was to isolate the primary targets of carbamylation in the lens and identify them by mass spectrometry...
  49. ncbi The N-terminal domain of betaB2-crystallin resembles the putative ancestral homodimer
    N J Clout
    Department of Crystallography, Birkbeck College, Malet Street, London, WC1E 7HX, UK
    J Mol Biol 304:253-7. 2000
    ..These results support the view that the betagamma-crystallin superfamily has evolved by a series of gene duplication and fusion events from a single-domain ancestor capable of forming homodimers...
  50. pmc Associations of seroreactivity against crystallin proteins with disease activity and cataract in patients with uveitis
    Ling Chen
    Departments of 2Ophthalmology, Ocular Inflammatory Disease Center, Jules Stein Eye Institute, University of California, Los Angeles, California 90095 7000, USA
    Invest Ophthalmol Vis Sci 49:4476-81. 2008
    ....
  51. pmc Mitochondrial proteomics in experimental autoimmune uveitis oxidative stress
    Sindhu Saraswathy
    Doheny Eye Institute, Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California 90033, USA
    Invest Ophthalmol Vis Sci 50:5559-66. 2009
    ..In this study, the authors determined alterations in retinal mitochondrial protein levels in response to oxidative stress during the early phase of experimental autoimmune uveitis (EAU)...
  52. pmc Truncated human betaB1-crystallin shows altered structural properties and interaction with human betaA3-crystallin
    K Srivastava
    Department of Vision Sciences, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Biochemistry 48:7179-89. 2009
    ....
  53. ncbi Targeted knockout of the mouse betaB2-crystallin gene (Crybb2) induces age-related cataract
    Jun Zhang
    Central Laboratory, Changhai Hospital, Second Military Medical University, Shanghai, People s Republic of China
    Invest Ophthalmol Vis Sci 49:5476-83. 2008
    ..It has been assumed that the gene functions as structural protein, although this has not been directly tested. Here the in vivo functions of betaB2-crystallin are examined via the generation of mice with a targeted disruption of Crybb2...
  54. ncbi Comprehensive mutational screening in a cohort of Danish families with hereditary congenital cataract
    Lars Hansen
    Wilhelm Johannsen Centre for Functional Genome Research, Institute of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark
    Invest Ophthalmol Vis Sci 50:3291-303. 2009
    ..Seven families have been published previously, and the data of the remaining 21 families are presented together with an overview of the results in all families...
  55. ncbi Disulfide cross-links in the interaction of a cataract-linked alphaA-crystallin mutant with betaB1-crystallin
    M Satish Kumar
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA
    FEBS Lett 583:175-9. 2009
    ..Covalent protein cross-links are a hallmark of age-related cataract and may be a factor in its inherited form...
  56. ncbi Association of partially folded lens betaB2-crystallins with the alpha-crystallin molecular chaperone
    Paul Evans
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Biochem J 409:691-9. 2008
    ..Our experimental results combined with previously reported observations of alpha-crystallin quaternary structure have led us to propose a structural model of how activated alpha-crystallin chaperones unfolded betaB2-crystallin...
  57. pmc Protein-protein interactions among human lens acidic and basic beta-crystallins
    Bing Fen Liu
    Center for Ophthalmic Research Surgery, Brigham and Women s Hospital, Department of Ophthalmology, Harvard Medical School, Boston, MA 02115, USA
    FEBS Lett 581:3936-42. 2007
    ..Circular dichroism indicates subtle differences in conformation among beta-crystallins that may have contributed to the differences in interactions...
  58. pmc Quantitative measurement of deamidation in lens betaB2-crystallin and peptides by direct electrospray injection and fragmentation in a Fourier transform mass spectrometer
    Noah E Robinson
    Oregon Institute of Science and Medicine, Cave Junction, OR 97523, USA
    Mol Vis 11:1211-9. 2005
    ..FTMS measurements of lens proteins from a 1-month-old human donor were also carried out...
  59. ncbi Recapitulation of human betaB1-crystallin promoter activity in transgenic zebrafish
    Hsin Han Hou
    Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China
    Dev Dyn 235:435-43. 2006
    ..These data suggest that the fundamental transcriptional regulatory mechanism of the betaB1-crystallin gene has been well conserved between humans and zebrafish, and plausibly among all vertebrates, during evolution...
  60. ncbi The sequence of human betaB1-crystallin cDNA allows mass spectrometric detection of betaB1 protein missing portions of its N-terminal extension
    L L David
    Department of Oral Molecular Biology, Oregon Health Sciences University, Portland, 97201, USA
    J Biol Chem 271:4273-9. 1996
    ....
  61. ncbi Energetics of domain-domain interactions and entropy driven association of beta-crystallins
    Y V Sergeev
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20982, USA
    Biochemistry 43:415-24. 2004
    ....
  62. ncbi Effect of oxidized betaB3-crystallin peptide on lens betaL-crystallin: interaction with betaB2-crystallin
    E G Padmanabha Udupa
    Department of Ophthalmology, University of Missouri, 65212, USA
    Invest Ophthalmol Vis Sci 46:2514-21. 2005
    ..To investigate the interaction of oxidized betaB3-crystallin peptide (residues 152-166) with betaL-crystallin and to identify peptide-interaction sites...
  63. ncbi Proteomic and sequence analysis of chicken lens crystallins reveals alternate splicing and translational forms of beta B2 and beta A2 crystallins
    Phillip A Wilmarth
    Department of Integrative Biosciences, Oregon Health and Science University, Portland, 97239, USA
    Invest Ophthalmol Vis Sci 45:2705-15. 2004
    ..To characterize the adult chicken lens proteome using mass spectrometry and two-dimensional gel electrophoresis (2-DE)...
  64. ncbi Requirement for betaB1-crystallin promoter of Xenopus laevis in embryonic lens development and lens regeneration
    Nobuhiko Mizuno
    Differentiation Transition Group, Kondoh Differentiation Signaling Project, ERATO, Japan Science and Technology Corporation, Kyoto 606 8305, Japan
    Dev Growth Differ 47:131-40. 2005
    ....
  65. ncbi Generation of transgenic newt Cynops pyrrhogaster for regeneration study
    Yoko Ueda
    Differentiation Transition Group, Kondoh Differentiation Signaling Project, ERATO, Japan Science and TechnologyCorporation, and Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
    Genesis 41:87-98. 2005
    ..The newt transgenesis technique described here is likely to be of wide use in monitoring and manipulating gene expression in the study of molecular mechanisms underlying tissue regeneration...
  66. ncbi Effect of oxidized betaB3-crystallin peptide (152-166) on thermal aggregation of bovine lens gamma-crystallins: identification of peptide interacting sites
    Padmanabha E G Udupa
    Department of Ophthalmology, EC 213, Mason Eye Institute, 1 Hospital Dr, Columbia, MO 65212, USA
    Exp Eye Res 80:185-96. 2005
    ..These results imply that oxidized betaB3-crystallin peptide interact with gamma-crystallins and enhance their aggregation and light scattering...
  67. ncbi Oligomerization and phase transitions in aqueous solutions of native and truncated human beta B1-crystallin
    Onofrio Annunziata
    Department of Physics, Center for Materials Science and Engineering, and Materials Processing Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Biochemistry 44:1316-28. 2005
    ....
  68. ncbi Study of the interaction between triplet riboflavin and the alpha-, betaH- and betaL-crystallins of the eye lens
    Gabriela Viteri
    , , Santiago, Chile
    Photochem Photobiol 77:535-40. 2003
    ..The correlations found in this study confirm the importance of the Type-I photosensitizing mechanism of the crystallins, when RF acts as a sensitizer at low oxygen concentration, as can occur in the eye lens...
  69. ncbi Rapid purification of recombinant betaB2-crystallin using hydrophobic interaction chromatography
    M K Jobby
    Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
    Protein Expr Purif 28:158-64. 2003
    ..Over all, the present protocol provides a rapid, efficient and simplified procedure for the preparation of betaB2-crystallin in large yield, sufficient for structural and functional studies...
  70. ncbi General utility of the chicken betaB1-crystallin promoter to drive protein expression in lens fiber cells of transgenic mice
    Jennifer R Taube
    Department of Biological Sciences, University of Delaware, Newark 19716, USA
    Transgenic Res 11:397-410. 2002
    ..Thus, the chicken betaB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ectopic proteins...
  71. ncbi Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins
    Maroor K Jobby
    Centre for Cellular and Molecular Biology CCMB, Hyderabad, India
    FEBS J 274:4135-47. 2007
    ..Our results have important implications for understanding the calcium-related cataractogenesis and maintenance of ionic homeostasis in the lens...
  72. pmc Specificity of alphaA-crystallin binding to destabilized mutants of betaB1-crystallin
    Hassane S McHaourab
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA
    FEBS Lett 581:1939-43. 2007
    ..In the lens, where alpha-crystallin binding sites are not regenerated, this may represent an added mechanism to maintain lens transparency...
  73. pmc Analysis of betaB1-crystallin unfolding equilibrium by spin and fluorescence labeling: evidence of a dimeric intermediate
    Hanane A Koteiche
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA
    FEBS Lett 581:1933-8. 2007
    ..This intermediate may be a critical determinant for the life-long stability of the beta-crystallins and has important consequences on interactions with alpha-crystallin...
  74. ncbi BetaB2-crystallin undergoes extensive truncation during aging in human lenses
    O P Srivastava
    Department of Physiological Optics, School of Optometry, University of Alabama at Birmingham, Worrell Building, 924 South 18th Street, Birmingham, AL 35294 4390, USA
    Biochem Biophys Res Commun 301:44-9. 2003
    ..The beta(H)-crystallin fraction also contained single fragments of alpha-, betaA4-, gammas-, and other gamma-crystallins...
  75. ncbi A study of complex formation by alpha- and betaL-crystallins under UV irradiation
    A V Krivandin
    Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul Kosygina 4, Moscow 119991, Russia
    Dokl Biochem Biophys 409:229-32. 2006
  76. ncbi In vivo heteromer formation. Expression of soluble betaA4-crystallin requires coexpression of a heteromeric partner
    Laura Marin-Vinader
    Department of Biochemistry, Radboud University Nijmegen, The Netherlands
    FEBS J 273:3172-82. 2006
    ..We suggest that the proper folding of betaA4-crystallin is not mediated by general chaperones but requires a heteromeric partner, which then also acts as a dedicated chaperone towards betaA4-crystallin...
  77. pmc Improving the performance of DomainParser for structural domain partition using neural network
    Jun tao Guo
    Protein Informatics Group, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830 6480, USA
    Nucleic Acids Res 31:944-52. 2003
    ..When compared with the manual decomposition results given in the SCOP database, our new algorithm achieves higher decomposition accuracy (81.9%) on the same data set...
  78. pmc Ubiquitin proteasome pathway-mediated degradation of proteins: effects due to site-specific substrate deamidation
    Edward J Dudek
    Laboratory for Nutrition and Vision Research, United States Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111 1524, USA
    Invest Ophthalmol Vis Sci 51:4164-73. 2010
    ..Among the roles of the ubiquitin proteasome pathway (UPP) is protein surveillance and maintenance of protein quality. The purpose of this study was to determine whether deamidation can alter clearance of crystallins by the UPP...
  79. pmc Initiation codon mutation in betaB1-crystallin (CRYBB1) associated with autosomal recessive nuclear pulverulent cataract
    Esther Meyer
    Department of Medical and Molecular Genetics, Institute of Biomedical Research, University of Birmingham, Birmingham, UK
    Mol Vis 15:1014-9. 2009
    ..To identify the molecular basis for autosomal recessively inherited congenital non-syndromic pulverulent cataracts in a consanguineous family with four affected children...
  80. ncbi Unfolding of human lens recombinant betaB2- and gammaC-crystallins
    Ling Fu
    Department of Ophthalmology, Center for Ophthalmic Research, Brigham and Women s Hospital, Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 139:191-8. 2002
    ..The significance of the thermodynamic stability for betaB2- and gammaC-crystallins may be related to their functions in the lens...
  81. pmc Solvent accessibility of betaB2-crystallin and local structural changes due to deamidation at the dimer interface
    Takumi Takata
    Oregon Health and Science University, Integrative Biosciences, 611 SW Campus Drive, Portland, OR 97239, USA
    Exp Eye Res 91:336-46. 2010
    ..The cumulative effects of multiple deamidation sites perturbing the structure both locally and distant from the site of deamidation may contribute to aggregation and precipitation during aging and cataractogenesis in the lens...
  82. ncbi Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of betaB2 crystallin onto the hydrophobic core of gammaB crystallin
    Divya Kapoor
    Division of Protein Science and Engineering, Institute of Microbial Technology, Chandigarh 160036, Council of Scientific and Industrial Research, New Delhi, India
    FEBS J 276:3341-53. 2009
    ..The folding of a protein with such a 'patchwork' residue ancestry suggests that interior/surface transplants involving all-beta proteins are a feasible engineering strategy...
  83. ncbi Beta-crystallin association
    J F Hejtmancik
    National Eye Institute NIH, Ophthalmic Genetics and Visual Function Branch, Building 10, Room 10B10, 10 Center Drive MSC 1860, Bethesda, MD 20892, USA
    Exp Eye Res 79:377-83. 2004
    ..As predicted by molecular modelling, the fraction of beta-crystallin present as dimers increases with increasing temperature, implying that beta-crystallin association is entropically driven...
  84. pmc Nonsense mutation in the CRYBB2 gene causing autosomal dominant progressive polymorphic congenital coronary cataracts
    Fei Feng Li
    Graduate School, Peking Union Medical College, Beijing, China
    Mol Vis 14:750-5. 2008
    ..We sought to identify the genetic defect in a large, five-generation Chinese family with autosomal dominant progressive polymorphic congenital coronary cataracts and to examine the clinical features in detail...

Research Grants3

  1. BIOMARKERS FOR TOTAL BODY BURDEN OF FLUORIDE
    Kirsten Lampi; Fiscal Year: 2004
    ..This research is a collaborative effort between Oregon Health Sciences University and the Medical College of Georgia. ..
  2. MECHANISMS OF CHAPERONE FUNCTION IN THE LENS
    Hassane McHaourab; Fiscal Year: 2008
    ..Reporter group spectroscopic approaches will be used to provide insight into the structural basis of recognition and binding. ..
  3. AUTOSOMAL DOMINANT CATARACTS
    J Bateman; Fiscal Year: 2004
    ..If the mutation is new experiments are proposed to test the validity of the hypothesis that the mutation is causative for the ADC by determining if the mutation causes cataracts in transgenic mice. ..