Genomes and Genes
green fluorescent proteins
Summary: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Publications326 found, 100 shown here
- Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeM S Longtine
Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
Yeast 14:953-61. 1998..Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae...
- Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locusS Srinivas
Department of Genetics and Development, Columbia University, New York, USA
BMC Dev Biol 1:4. 2001....
- Lifeact: a versatile marker to visualize F-actinJulia Riedl
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
Nat Methods 5:605-7. 2008..Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells...
- A global double-fluorescent Cre reporter mouseMandar Deepak Muzumdar
Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA
Genesis 45:593-605. 2007..In addition to serving as a global Cre reporter, the mT/mG mouse may also be used as a tool for lineage tracing, transplantation studies, and analysis of cell morphology in vivo...
- Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteinsG Miesenbock
Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 394:192-5. 1998..When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules...
- Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertionS Jung
Skirball Institute of Biomolecular Medicine and Howard Hughes Medical Institute New York University Medical Center, New York, New York 10016, USA
Mol Cell Biol 20:4106-14. 2000..Furthermore, a prominent response of CX(3)CR1-deficient microglia to peripheral nerve injury indicates unimpaired neuronal-glial cross talk in the absence of CX(3)CR1...
- Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFPG Feng
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Neuron 28:41-51. 2000..In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal...
- A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and functionJohn D Cahoy
Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
J Neurosci 28:264-78. 2008....
- A gene expression atlas of the central nervous system based on bacterial artificial chromosomesShiaoching Gong
GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
Nature 425:917-25. 2003..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community...
- Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3Shunsuke Kimura
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
Autophagy 3:452-60. 2007..This method will be of general utility for analysis of the autophagosome maturation process...
- In vivo imaging of embryonic vascular development using transgenic zebrafishNathan D Lawson
Laboratory of Molecular Genetics, NICHD NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA
Dev Biol 248:307-18. 2002....
- The green fluorescent proteinR Y Tsien
Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093 0647, USA
Annu Rev Biochem 67:509-44. 1998..Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories...
- Single-copy insertion of transgenes in Caenorhabditis elegansChristian Frøkjaer-Jensen
Department of Biology and Howard Hughes Medical Institute, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112, USA
Nat Genet 40:1375-83. 2008..We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines...
- Spatiotemporal rescue of memory dysfunction in DrosophilaSean E McGuire
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 302:1765-8. 2003..The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required...
- Ultra-high resolution imaging by fluorescence photoactivation localization microscopySamuel T Hess
Department of Physics and Astronomy, University of Maine, Orono, ME 04469, USA
Biophys J 91:4258-72. 2006..This new method suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution...
- 'Green mice' as a source of ubiquitous green cellsM Okabe
Research Institute for Microbial Diseases, Osaka University, Suita, Japan
FEBS Lett 407:313-9. 1997..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...
- Engineering and characterization of a superfolder green fluorescent proteinJean Denis Pédelacq
Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Nat Biotechnol 24:79-88. 2006..X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP...
- Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectorsCarlos Lois
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Science 295:868-72. 2002..We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species...
- Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformationTsuyoshi Nakagawa
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 1060 Nishikawatsu, Matsue, Japan
J Biosci Bioeng 104:34-41. 2007..Moreover, tissue-specific and inducible gene expression using a promoter was also monitored with pGWBs. It is expected that, the pGWB system will serve as a powerful tool for plasmid construction in plant research...
- Directed differentiation of embryonic stem cells into motor neuronsHynek Wichterle
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
Cell 110:385-97. 2002..Thus, inductive signals involved in normal pathways of neurogenesis can direct ES cells to form specific classes of CNS neurons...
- Stochastic gene expression in a single cellMichael B Elowitz
Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
Science 297:1183-6. 2002..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...
- Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cellsDavid A Zacharias
Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
Science 296:913-6. 2002..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
- Improving the photostability of bright monomeric orange and red fluorescent proteinsNathan C Shaner
Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
Nat Methods 5:545-51. 2008..and TagRFP (a monomeric derivative of eqFP578 from Entacmaea quadricolor) that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs...
- An expanded palette of genetically encoded Ca²⁺ indicatorsYongxin Zhao
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
Science 333:1888-91. 2011..This palette of indicators paints the way to a colorful new era of Ca(2+) imaging...
- Defining the epithelial stem cell niche in skinTudorita Tumbar
Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
Science 303:359-63. 2004..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
- FACS-optimized mutants of the green fluorescent protein (GFP)B P Cormack
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305 5402, USA
Gene 173:33-8. 1996..coli, the folding of the mutant proteins is more efficient than folding of wt GFP. These two properties contribute to a greatly increased (100-fold) fluorescence intensity, making the mutants useful for a number of applications...
- Bone marrow cells regenerate infarcted myocardiumD Orlic
Hematopoiesis Section, Genetics and Molecular Biology Branch, NHGRI, NIH, Bethesda, MD 20892, USA
Nature 410:701-5. 2001..The developing tissue comprised proliferating myocytes and vascular structures. Our studies indicate that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease...
- Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulinA Miyawaki
Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
Nature 388:882-7. 1997..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
- Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouseNobuaki Tamamaki
Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
J Comp Neurol 467:60-79. 2003..4% of the GFP-positive cells. They accounted for 6.5% in layer I. In accord with unidentified GFP-positive cells, many medium-sized spherical somata emitting intense GFP fluorescence were observed in layer I...
- The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructsKristen M Kwan
Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah, USA
Dev Dyn 236:3088-99. 2007..The Tol2kit greatly facilitates zebrafish transgenesis, simplifies the sharing of clones, and enables large-scale projects testing the functions of libraries of regulatory or coding sequences...
- Green fluorescent protein as a marker for gene expressionM Chalfie
Department of Biological Sciences, Columbia University, New York, NY 10027
Science 263:802-5. 1994..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms...
- A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteinsUlrich Rothbauer
Munich Center for Integrated Protein Science, CiPS M and Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
Mol Cell Proteomics 7:282-9. 2008b>Green fluorescent proteins (GFPs) and variants thereof are widely used to study protein localization and dynamics...
- Functional neurogenesis in the adult hippocampusHenriette van Praag
Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Nature 415:1030-4. 2002..Our findings demonstrate that newly generated cells mature into functional neurons in the adult mammalian brain...
- Automatic and quantitative measurement of protein-protein colocalization in live cellsSylvain V Costes
Image Analysis Laboratory, National Cancer Institute, Frederick, Maryland, USA
Biophys J 86:3993-4003. 2004..25 x 10(-3) s(-1). More generally, this algorithm can be used to answer a variety of biological questions involving protein-protein interactions or co-compartmentalization and can be generalized to colocalization of more than two colors...
- Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytesKevin D Foust
Center for Gene Therapy, The Research Institute at Nationwide Children s Hospital, 700 Children s Drive, Columbus, Ohio 43205, USA
Nat Biotechnol 27:59-65. 2009..It may also be useful for rapid postnatal genetic manipulations in basic neuroscience studies...
- Local, efflux-dependent auxin gradients as a common module for plant organ formationEva Benkova
Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
Cell 115:591-602. 2003..Our results suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin...
- A high signal-to-noise Ca(2+) probe composed of a single green fluorescent proteinJ Nakai
Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki, 444 8585, Japan
Nat Biotechnol 19:137-41. 2001..G-CaMP will be a useful tool for visualizing intracellular Ca2+ in living cells. Mutational analysis, together with previous structural information, suggests the residues that may alter the fluorescence of GFP...
- An improved zinc-finger nuclease architecture for highly specific genome editingJeffrey C Miller
Sangamo BioSciences, Inc, Pt Richmond Tech Center, 501 Canal Blvd, Suite A100 Richmond, California 94804, USA
Nat Biotechnol 25:778-85. 2007..This architecture provides a general means for improving the specificity of ZFNs as gene modification reagents...
- Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiaeMark A Sheff
Bauer Center for Genomics Research, 7 Divinity Avenue, Harvard University, Cambridge, MA 02138, USA
Yeast 21:661-70. 2004..These tags significantly improve the detection limits for live-cell fluorescence imaging in yeast, and provide sufficient distinguishable fluorophores for four-colour imaging...
- A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafishKoichi Kawakami
Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
Dev Cell 7:133-44. 2004..We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis...
- Localization of ASH1 mRNA particles in living yeastE Bertrand
Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Mol Cell 2:437-45. 1998..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
- An improved cyan fluorescent protein variant useful for FRETMark A Rizzo
Nat Biotechnol 22:445-9. 2004..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
- XRCC3 promotes homology-directed repair of DNA damage in mammalian cellsA J Pierce
Cell Biology Program, Memorial Sloan Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
Genes Dev 13:2633-8. 1999..These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal...
- Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modificationMorgan L Maeder
Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
Mol Cell 31:294-301. 2008..In summary, OPEN provides an "open-source" method for rapidly engineering highly active zinc-finger arrays, thereby enabling broader practice, development, and application of ZFN technology for biological research and gene therapy...
- High-throughput in vivo analysis of gene expression in Caenorhabditis elegansRebecca Hunt-Newbury
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada
PLoS Biol 5:e237. 2007..elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org)...
- Circularly permuted green fluorescent proteins engineered to sense Ca2+T Nagai
Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, Institute of Physical and Chemical Research (RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Proc Natl Acad Sci U S A 98:3197-202. 2001..The Ca(2+)-dependent interaction between calmodulin and M13 in HeLa cells was monitored by the association of the two halves of GFP, neither of which was fluorescent by itself...
- Decapping and decay of messenger RNA occur in cytoplasmic processing bodiesUjwal Sheth
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
Science 300:805-8. 2003..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
- Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegansL Timmons
Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
Gene 263:103-12. 2001....
- CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearanceJan Hendrik Niess
Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
Science 307:254-8. 2005..Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation...
- Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleusM A Cowley
The Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA
Nature 411:480-4. 2001..On the basis of our results, we propose an integrated model of leptin action and neuronal architecture in the arcuate nucleus of the hypothalamus...
- Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissuesKonrad Hochedlinger
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 121:465-77. 2005..These data show that certain adult progenitors remain competent to interpret key embryonic signals and support the notion that progenitor cells are a driving force in tumorigenesis...
- High efficiency transfection of Plasmodium berghei facilitates novel selection proceduresChris J Janse
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
Mol Biochem Parasitol 145:60-70. 2006..The combination of enhanced transfection efficiency and a versatile rodent model provides a basis for the further development of novel tools for high throughput genome manipulation...
- Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryosAlexander Pfeifer
The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:2140-5. 2002..Ability to transfer foreign genes into human ES cells has potential relevance for the development of gene and cell-based therapies...
- Neural stem and progenitor cells in nestin-GFP transgenic miceJohn L Mignone
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
J Comp Neurol 469:311-24. 2004....
- The carnegie protein trap library: a versatile tool for Drosophila developmental studiesMichael Buszczak
Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
Genetics 175:1505-31. 2007....
- Direct isolation of satellite cells for skeletal muscle regenerationDidier Montarras
CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France
Science 309:2064-7. 2005..Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity...
- Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicatorsGeorge T Hanson
Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
J Biol Chem 279:13044-53. 2004..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix...
- Receptor-mediated endocytosis in the Caenorhabditis elegans oocyteB Grant
Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
Mol Biol Cell 10:4311-26. 1999..We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor...
- Fluorescent proteins and their applications in imaging living cells and tissuesDmitriy M Chudakov
Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
Physiol Rev 90:1103-63. 2010..Here we focus on the structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging, with particular attention to recent techniques...
- Visualization of the intracellular behavior of HIV in living cellsDavid McDonald
Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
J Cell Biol 159:441-52. 2002..Based on these observations, we propose that HIV uses dynein and the microtubule network to facilitate the delivery of the viral genome to the nucleus of the cell during early postentry steps of the HIV life cycle...
- Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous systemCorinna Burger
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville 32610, USA
Mol Ther 10:302-17. 2004..Retrograde transport of rAAV1 and rAAV5 was also observed in particular CNS areas. These results suggest that vectors based on distinct AAV serotypes can be chosen for specific applications in the nervous system...
- Electroporation and RNA interference in the rodent retina in vivo and in vitroTakahiko Matsuda
Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 101:16-22. 2004..Plasmid DNA was also efficiently transfected into retinal explants in vitro by high-voltage pulses...
- Use of the viral 2A peptide for bicistronic expression in transgenic miceGeorgios Trichas
Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, UK
BMC Biol 6:40. 2008..They work in a wide variety of eukaryotic cells, but to date have not been demonstrated to function in transgenic mice in an inheritable manner...
- The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequencesM L Donnelly
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 82:1027-41. 2001....
- Optical sectioning deep inside live embryos by selective plane illumination microscopyJan Huisken
European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Science 305:1007-9. 2004..We also demonstrate that SPIM can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo...
- Capturing and profiling adult hair follicle stem cellsRebecca J Morris
Department of Dermatology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
Nat Biotechnol 22:411-7. 2004..Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair...
- A modular design for the clathrin- and actin-mediated endocytosis machineryMarko Kaksonen
Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
Cell 123:305-20. 2005....
- Movement of Bax from the cytosol to mitochondria during apoptosisK G Wolter
Biochemistry Section, Surgical Neurology Branch, Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
J Cell Biol 139:1281-92. 1997....
- High-level transduction and gene expression in hematopoietic repopulating cells using a human immunodeficiency [correction of imunodeficiency] virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoterChristophe Demaison
Molecular Immunology Unit, Institute of Child Health, UCL, 30 Guilford Street, London, WC1N 1EH, UK
Hum Gene Ther 13:803-13. 2002....
- A critical period for enhanced synaptic plasticity in newly generated neurons of the adult brainShaoyu Ge
Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Neuron 54:559-66. 2007....
- Disruption of fusion results in mitochondrial heterogeneity and dysfunctionHsiuchen Chen
Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
J Biol Chem 280:26185-92. 2005..In contrast, fragmentation of mitochondria, without severe loss of fusion, did not result in such cellular defects. Our results showed that key cellular functions decline as mitochondrial fusion is progressively abrogated...
- Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like diseaseKatharina Lahl
Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
J Exp Med 204:57-63. 2007..Furthermore, DEREG mice will allow a more precise definition of the function of Foxp3+ T reg cells in immune reactions in vivo...
- Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophagesN Faust
Albert Einstein College of Medicine, Bronx, NY 10461, USA
Blood 96:719-26. 2000..Mice with an inactivation of both copies of the lys gene developed normally and were fertile. (Blood. 2000;96:719-726)..
- Engineering stability in gene networks by autoregulationA Becskei
EMBL, Structures and Biocomputing, Heidelberg, Germany
Nature 405:590-3. 2000..Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback...
- Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellumMikio Hoshino
Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Neuron 47:201-13. 2005..Our results suggest that Ptf1a is involved in driving neural precursors to differentiate into GABAergic neurons in the cerebellum...
- Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical periodBidisha Chattopadhyaya
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
J Neurosci 24:9598-611. 2004....
- Genome-scale spatiotemporal analysis of Caenorhabditis elegans microRNA promoter activityNatalia J Martinez
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genome Res 18:2005-15. 2008..Finally, our data suggest that post-transcriptional mechanisms contribute to differential miRNA expression. The data and strains described here will provide a valuable guide and resource for the functional analysis of C. elegans miRNAs...
- Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesisWulf Haubensak
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
Proc Natl Acad Sci U S A 101:3196-201. 2004..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
- Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicatorsHiromi Imamura
Precursory Research for Embryonic Science, Japan Science and Technology Agency, 5 Sanbancho, Chiyoda ku, Tokyo 102 0075, Japan
Proc Natl Acad Sci U S A 106:15651-6. 2009..This was also confirmed by an experiment using oligomycin A, an inhibitor of F(o)F(1)-ATP synthase. In addition, it was demonstrated that HeLa cells change ATP-generating pathway in response to changes of nutrition in the environment...
- Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cellsTakaki Miyata
Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, Saitama 351 0198, Japan
Development 131:3133-45. 2004..The asymmetric P/P division may therefore contribute to efficient neuron/progenitor segregation required for CP initiation through cell cycle-dependent and lineage-restricted expression of Ngn2...
- Effective targeted gene 'knockdown' in zebrafishA Nasevicius
Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA
Nat Genet 26:216-20. 2000..Conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebrafish embryos...
- Translational control and target recognition by Escherichia coli small RNAs in vivoJohannes H Urban
Max Planck Institute for Infection Biology, RNA Biology Group, Chariteplatz 1, 10117 Berlin, Germany
Nucleic Acids Res 35:1018-37. 2007..We expect our GFP fusion approach to be applicable to sRNA targets of other bacteria, and also demonstrate that Vibrio RyhB sRNA represses a Vibrio sodB fusion when co-expressed in E.coli...
- A simplified system for generating recombinant adenovirusesT C He
The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 95:2509-14. 1998..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
- A synthetic oscillatory network of transcriptional regulatorsM B Elowitz
Department of Molecular Biology and Physics, Princeton University, New Jersey 08544, USA
Nature 403:335-8. 2000..Such 'rational network design may lead both to the engineering of new cellular behaviours and to an improved understanding of naturally occurring networks...
- Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafishJon M Davison
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Dev Biol 304:811-24. 2007....
- Processing bodies require RNA for assembly and contain nontranslating mRNAsDaniela Teixeira
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721 0106, USA
RNA 11:371-82. 2005..Fourth, multiple translation factors and ribosomal proteins are lacking from P-bodies. These results suggest additional biological roles of P-bodies in addition to being sites of mRNA degradation...
- Development and use of fluorescent protein markers in living cellsJennifer Lippincott-Schwartz
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Science 300:87-91. 2003....
- A novel eGFP-expressing immunodeficient mouse model to study tumor-host interactionsSimone P Niclou
CRP Sante, Norlux Neuro Oncology Laboratory, 84, Val Fleuri, L 1526 Luxembourg
FASEB J 22:3120-8. 2008..Thus, the model described should provide significant insight into how tumor cells communicate with their microenvironment...
- Atg8 controls phagophore expansion during autophagosome formationZhiping Xie
Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Mol Biol Cell 19:3290-8. 2008..This model provides a foundation for future analyses of the functions and dynamics of known autophagy-related proteins and for screening new genes...
- Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality controlAdam C Fisher
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States of America
PLoS ONE 3:e2351. 2008....
- daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegansS T Henderson
Institute for Behavioral Genetics, University of Colorado at Boulder, Campus Box 447, Boulder, CO 80309 0447, USA
Curr Biol 11:1975-80. 2001..We suggest that changes in the subcellular localization of DAF-16 by environmental cues allows for rapid reallocation of resources in response to a changing environment at all stages of life...
- Effective gene therapy with nonintegrating lentiviral vectorsRafael J Yáñez-Muñoz
Molecular Immunology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
Nat Med 12:348-53. 2006..For therapeutic application to postmitotic tissues, this system substantially reduces the risk of insertional mutagenesis...
- Identification of the nuclear localization and export signals of high risk HPV16 E7 oncoproteinAlixandra A Knapp
Biology Department, Boston College, Higgins Hall, Room 578, 140 Commonwealth Avenue, Chestnut Hill, MA 02467, USA
Virology 383:60-8. 2009..The presence of both NLSs and an NES suggests that HPV16 E7 shuttles between the cytoplasm and nucleus which is consistent with E7 having functions in both of these cell compartments...
- A role for NBR1 in autophagosomal degradation of ubiquitinated substratesVladimir Kirkin
Institute of Biochemistry II, Goethe University, Frankfurt Main, Germany
Mol Cell 33:505-16. 2009..We propose that NBR1 and p62 act as receptors for selective autophagosomal degradation of ubiquitinated targets...
- Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathwayEek Hoon Jho
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
Mol Cell Biol 22:1172-83. 2002..Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal...
- APC and GSK-3beta are involved in mPar3 targeting to the nascent axon and establishment of neuronal polaritySong Hai Shi
Departments of Physiology and Biochemistry, Howard Hughes Medical Institute, University of California at San Francisco, 1550 4th Street, San Francisco, CA 94143 0725, USA
Curr Biol 14:2025-32. 2004....
- Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cellsPaul Gadue
Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1496, New York, NY 10029, USA
Proc Natl Acad Sci U S A 103:16806-11. 2006....
- Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virusQian Zhang
Genelux Corporation, San Diego Science Center, San Diego, California 92109, USA
Cancer Res 67:10038-46. 2007..These findings suggest that immune activation may combine with viral oncolysis to induce tumor eradication in this model, providing a novel perspective for the design of oncolytic viral therapies for human cancers...
- Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behaviorHenry J Klassen
Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
Invest Ophthalmol Vis Sci 45:4167-73. 2004..To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration...
- Novel AAV serotypes for improved ocular gene transferCorinna Lebherz
Gene Therapy Program, Division of Medical Genetics, Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA
J Gene Med 10:375-82. 2008..We show here that AAV7 and 8 enable superior long-term transduction of retinal and also anterior chamber structures...
- Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarctionYoshinori Miyahara
Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
Nat Med 12:459-65. 2006..Thus, transplantation of monolayered mesenchymal stem cells may be a new therapeutic strategy for cardiac tissue regeneration...
- Assessing Protein Interactions in Cells by FluorescenceWilliam Clark; Fiscal Year: 2003..By fusing blue and green fluorescent proteins (BFP, GFP and/or YFP/CFP) to the receptor chains and expressing them in cells, these fluorophores could ..
- Structure-based Directed Evolution of Fast-Maturing GFPsRebekka Wachter; Fiscal Year: 2007..We propose to develop fast-maturing GFPs (green fluorescent proteins) by directed evolution, as guided by structural and mechanistic knowledge...
- The relative roled of sex and genes during development of systemic autoimmunityTRINE NORGAARD JORGENSEN; Fiscal Year: 2010..To do so, we will target immune cells of a given sex with green fluorescent proteins prior to transfer into sex-matched or mismatched lethally irradiated recipients...
- A novel model for studying fetal skeletal muscle developmentMin Du; Fiscal Year: 2009..pDsRed and pAcGFP vectors are mammalian expression vectors and express red and green fluorescent proteins respectively...
- Electrostatics and Dynamics in ProteinsSteven G Boxer; Fiscal Year: 2013..information on fields and their effects on function at the active sites of seveal enzymes and green fluorescent proteins (GFPs)...
- Small Animal Tomography System for Green Fluorescent Protein ImagingAndreas H Hielscher; Fiscal Year: 2011..this proposal is to develop image reconstruction schemes and instrumentation for tomographic imaging of green fluorescent proteins (GFP) in small animals...
- High Speed 10-Color Flow CytometerDEBRA LYNN LASKIN; Fiscal Year: 2012..Paired with the use of monoclonal antibodies, green fluorescent proteins, and other fluorescent indicator dyes, cell subsets have been identified and characterized that have led ..
- (PQD5) Development of in Vitro Vascularized Microtumors for Drug ScreeningChristopher C W Hughes; Fiscal Year: 2013..In addition, lentiviral-mediated expression of red, yellow and green fluorescent proteins in the tumor cells, endothelial cells and stromal cells will allow for repeate, rapid and quantitative ..
- IONIC CONTROL OF CARDIAC MUSCLE CONTRACTION: EC COUPLINGDonald Bers; Fiscal Year: 2004..This will be examined using new fluorescence resonance energy transfer probes, based on modified green fluorescent proteins linked by calmodulin biding peptides. This will provide fundamental new data about this system. 3...
- 3 to15 Covalent Gold for High Resolution Electron MicroscopyVISHWAS JOSHI; Fiscal Year: 2009..Clonable expression tags will be used in which yellow and green fluorescent proteins (YFP and GFP respectively) are expressed in combination with hexahistidine tag (His-tag) and lac ..
- MYOFIBRILLOGENESIS IN LIVING CARDIAC MUSCLE CELLSJoseph Sanger; Fiscal Year: 2006..The experimental approaches include the use of plasmids encoding members of the group of Green Fluorescent Proteins ligated to sarcomeric proteins and mutated domains for transfections of cardiomyocytes...
- Small Animal Tomography System for Green Fluorescent Protein ImagingANDREAS HIELSCHER; Fiscal Year: 2007..this proposal is to develop image reconstruction schemes and instrumentation for tomographic imaging of green fluorescent proteins (GFP) in small animals...
- Live Cell Imaging with Fluorescent Polymer BioconjugatesJOONGHO MOON; Fiscal Year: 2005..commonly used fluorophores in cell imaging, cell tracking, and in vivo imaging are organic molecules or green fluorescent proteins (GFPs). They have proven valuable in optical imaging and been applied to the study of single molecules...
- MYOFIBRILLOGENESIS IN LIVING SKELETAL MUSCLE CELLSJoseph Sanger; Fiscal Year: 2004..single living skeletal muscle cells transfected with probes expressing sarcomeric proteins linked to Green Fluorescent Proteins. Our second approach is focused on a molecular analysis of proteins interacting with the Z-bodies and Z-..
- GASTRIC NEUROPEPTIDASES/PEPTIDE RECEPTOR INTERACTIONSCatia Sternini; Fiscal Year: 2002..sites and pH at which these occur; and d) identifying interactions between receptors tagged with green fluorescent proteins, Cy3 labeled GRP, and other molecules that are colocalized at different stages of endocytosis and ..
- Stereology & Live Cell Imaging WorkstationAnda Cornea; Fiscal Year: 2004..For other projects, use of green fluorescent proteins and their mutants has created opportunities for identifying the intracellular location, translocation ..
- Mosiac Analysis of Synaptic Development in MiceGuoping Feng; Fiscal Year: 2006..generated many lines of transgenic mice in which a small number of individual neurons are labeled with green fluorescent proteins (GFP) in a Golgi-like fashion, allowing novel analyses of processes such as axonal guidance, dendritic ..
- YEAST BASED NUCLEAR REPORTER LIFETIME FRET ASSAY SYSTEMSalvador Fernandez; Fiscal Year: 2000..The approach is based on the use of green fluorescent proteins as an intracellular market, and fluorescence lifetime sensing to quantify reporter gene expression...
- ACQUISITION OF A HIGH SPEED 7 COLOR FACS INSTRUMENTTroy Randall; Fiscal Year: 2001..to examine UV dependent parameters, such as low level Ca2+ flux, Hoescht staining of DNA, and some types of green fluorescent proteins. Therefore, we feel that the MoFlo is the instrument of choice for the Institute's investigators.
- Orthotopic models of tumor angiogenesis and blood flowMeng Yang; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Imageable tumor-targeting bacteriaMing Zhao; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Enzyme therapy for hyperhomocysteinemiaYuying Tan; Fiscal Year: 2005..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
- Targeted tumoricidal bacteriaMing Zhao; Fiscal Year: 2007..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
- Dual-color tumor-host imaging modelsMeng Yang; Fiscal Year: 2007..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
- Discovery of novel fluorescent reporter genesMing Zhao; Fiscal Year: 2004..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
- Therapeutic hair follicle-derived neurospheresMeng Yang; Fiscal Year: 2007..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
- Adenoviral GFP targeting of metastatic human tumors using multiple delivery routeHiroyuki Kishimoto; Fiscal Year: 2008..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
- Inhibitors of 5alpha-reductase for acne therapyLingna Li; Fiscal Year: 2002..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
- RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAYYuying Tan; Fiscal Year: 2002..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
- NEURAL REGULATION OF VASOPRESSIN RELEASEJ Thomas Cunningham; Fiscal Year: 2010..Benefits: The results will provide new information regarding the control of vasopressin secretion and how these systems contribute to pathophysiology. ..
- CONTROL OF SODIUM INTAKE IN THE HINDLIMB UNWEIGHTED RATJ Cunningham; Fiscal Year: 2005..Additionally, these data may contribute to understanding of health problems associated with an aging population and with cardiovascular disease such as congestive heart failure. ..
- LENTIVIRAL DELIVERY OF GDNF AND BCL-2 IN PD MODELMarina Emborg; Fiscal Year: 2003..These studies will serve as the preclinical foundation to determine whether this in vivo method to deliver these potent trophic factor and antiapoptotic genes will be suitable for testing in patients with PD. ..
- ACYLTRANSFERASE INHIBITORS AS ANTI RAS AGENTSCharles Smith; Fiscal Year: 2009..abstract_text> ..
- Mathematical Models of Programmed CTL ProliferationDominik Wodarz; Fiscal Year: 2009..These are the role of effector molecules (such as perform and IFN-g) for CTL dynamics, and the role of CD4 T cell help. The modeling work will be complemented by experiments with VSV and LCMV infected mice. ..
- NPY DEPRESSION OF HIPPOCAMPAL EPILEPSYAnthony van den Pol; Fiscal Year: 2002..NPY is potentially of great interest because its primary action in the normal hippocampus appears to be one of depressing hyperexcited neurons, without a substantial effect on normal neurotransmission. ..
- Hypocretin NeuronsAnthony N van den Pol; Fiscal Year: 2010..Understanding the role of the endogenous opioid in hypocretin cells should also facilitate the understanding and treatment of narcolepsy. ..
- Pitx2 Function in Developing Mouse Brain NeuronsDonna M Martin; Fiscal Year: 2010..These results will help guide future experiments aimed at directing neural stem cell differentiation toward specific neuronal cell subtypes. ..
- GENISTEIN AS MODULATOR OF ANTIOXIDANT PROTEIN EXPRESSIONShiu Ming Kuo; Fiscal Year: 2002..These studies may also lead to the identification of a previous unknown pathway for Gn to regulate mammalian genes. ..
- Cytomegalovirus in the BrainAnthony N van den Pol; Fiscal Year: 2010..These experiments will help us understand the mechanisms associated with CMV-induced neurological dysfunction, and how to combat the virus within the brain with minimal complications. ..
- Elucidate C. elegans Oxygen Sensing with MicrofluidicsHang Lu; Fiscal Year: 2006..The general techniques developed in this study should impact other C. elegans researches of sensing and behaviors such as odor discrimination and thermo-sensation. ..
- Gene drive potential of transposable elements in Anopheles gambiae.DAVID A O apos BROCHTA; Fiscal Year: 2010..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
- Function and regulation of T-box factors in C. elegansPeter G Okkema; Fiscal Year: 2010..elegans, and the results obtained will have a direct bearing on our understanding of these factors in all organisms. ..
- Regulation of SMAD4 and BMPR1A Expression in Juvenile PolyposisJames R Howe; Fiscal Year: 2010..abstract_text> ..
- STRUCTURE AND FUNCTION OF THE LH AND FSH RECEPTORSDeborah Segaloff; Fiscal Year: 2002..Long term goals of research in this area include the ability to better understand, diagnose, and correct reproductive disorders. ..
- Conference on Photodynamic TherapyDavid Kessel; Fiscal Year: 2002..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
- MOLECULAR ANGLE TRANSITIONS IN MUSCLETHOMAS BURGHARDT; Fiscal Year: 2001....
- Imageable Mouse Models of Pancreatic CancerMichael Bouvet; Fiscal Year: 2008..Using such dual-colored imaging techniques, a better characterization of the tumor-host interactions in pancreatic cancer can be made. ..
- Gonadotropin Receptor Dimerization/OligomerizationDeborah Segaloff; Fiscal Year: 2008..abstract_text> ..
- Function of a Neuropeptide Gene Family in C. elegansChristine Li; Fiscal Year: 2006..In addition, because C. elegans is the current genetic model system for parasitic nematodes, elucidating the functions of neuropeptides and their signaling pathways may identify new antihelmintic drug targets. ..
- Proteomic charaterization of insulin signaling targetsJONATHAN BOGAN; Fiscal Year: 2006..Additionally, this work may have broader implications for insulin signaling and for protein targeting. [unreadable] [unreadable]..
- Redox control of Nrf2/ARE by thioredoxin and GSHJason Hansen; Fiscal Year: 2005..The roles of GSH and cytosolic and nuclear TRX during NRF2 activation and binding will provide an essential foundation for future developmental toxicology. ..
- MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLYLynne Cassimeris; Fiscal Year: 2006..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
- Novel & Selective Small Molecular Inhibitors of Human Peptide DeformylaseHakim Djaballah; Fiscal Year: 2006..Indeed, the likelihood that a compound interferes with both assays, and therefore behaves as a false positive in both assays, is vastly reduced compared to a strategy solely based on one technique. ..
- Genetic Reg. of Hematopoietic Stem Cell MobilizationHartmut Geiger; Fiscal Year: 2007..g. to muscle tissue, or to other hematopoietic niches. ..
- DRUG DISCOVERY CORE FACILITYCharles Smith; Fiscal Year: 2005..Establishment of this DDCF will provide an important bridge between basic and applied research, and so will enhance the research environment and advance cancer research. ..
- Cleveland Cell Biology Symposium: RegulationALAN TARTAKOFF; Fiscal Year: 2004..The results should be an intense synthesis of knowledge which will be stimulating for both students and established faculty. ..
- Role of Pitx2 in the Mammalian Central Nervous SystemDonna Martin; Fiscal Year: 2005..5-14.5) mouse diencephalon, 2) Develop and analyze mice with Pitx2 loss of function, and 3) Develop and analyze transgenic mice with CNS-specific Pitx2 gain of function. ..
- PHARYNGEAL MYOGENESIS AND ORGANOGENESIS IN C ELEGANSPETER OKKEMA; Fiscal Year: 2004..Finally we are specifically examining role the PEB-l plays during pharyngeal development by isolating and characterizing apeb-1 mutant. ..
- IGF-I and its binding proteins in vascular smooth muscle cellsCunming Duan; Fiscal Year: 2008..unreadable] [unreadable]..
- Dissecting neural circuits by microfluidic-enabled rapid microscopy & ablationHang Lu; Fiscal Year: 2008..In addition, besides the contribution to C. elegans sensory biology, the technologies are widely applicable to areas such as developmental biology, and to other organisms. [unreadable] [unreadable]..
- Forward and Reverse Genetics in Mosquitoes Using an RNA VirusZACH ADELMAN; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENEPaul Robbins; Fiscal Year: 2002..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
- 2008 Viral Vectors for Gene Therapy, The Science of Gordon Research ConferencePaul Robbins; Fiscal Year: 2008..End of Abstract) [unreadable] [unreadable] [unreadable]..
- Inhibition of NF-KB to Facilitate Islet TransplantationPaul Robbins; Fiscal Year: 2003..The successful completion of the proposed studies should determine if NF-kB inhibition in islets by peptide and gene mediated transduction is able to improve islet viability prior to and post-transplantation. ..
- Assemby and Recombination of dsRNA Bacteriophage phi8Leonard Mindich; Fiscal Year: 2004..Since reverse genetic techniques and in vitro genomic packaging are not available for these viruses, the cystoviridae (dsRNA bacteriophages) offer a valuable model for system for the study of the eukaryotic systems. ..