viral fusion proteins

Summary

Summary: Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.

Top Publications

  1. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
  2. pmc Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme
    Judith M White
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    Crit Rev Biochem Mol Biol 43:189-219. 2008
  3. ncbi Crystal structure of glycoprotein B from herpes simplex virus 1
    Ekaterina E Heldwein
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Science 313:217-20. 2006
  4. pmc Structural basis for immunization with postfusion respiratory syncytial virus fusion F glycoprotein (RSV F) to elicit high neutralizing antibody titers
    Kurt A Swanson
    Novartis Vaccines and Diagnostics, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 108:9619-24. 2011
  5. pmc Antigenic and genetic variability of human metapneumoviruses
    Bernadette G van den Hoogen
    Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Emerg Infect Dis 10:658-66. 2004
  6. pmc The primed ebolavirus glycoprotein (19-kilodalton GP1,2): sequence and residues critical for host cell binding
    Derek Dube
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    J Virol 83:2883-91. 2009
  7. pmc Class III viral membrane fusion proteins
    Marija Backovic
    Department of Virology, Pasteur Institute, 25 rue du Dr Roux, Paris, France
    Curr Opin Struct Biol 19:189-96. 2009
  8. ncbi Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation
    Hsien Sheng Yin
    Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nature 439:38-44. 2006
  9. ncbi Respiratory syncytial virus with the fusion protein as its only viral glycoprotein is less dependent on cellular glycosaminoglycans for attachment than complete virus
    Sunee Techaarpornkul
    Department of Biopharmacy, Silpakorn University, Sanamchan Palace Campus, Nakornpathom, 73000, Thailand
    Virology 294:296-304. 2002
  10. ncbi Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus
    E A Kurt-Jones
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Nat Immunol 1:398-401. 2000

Detail Information

Publications276 found, 100 shown here

  1. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
    ..These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion...
  2. pmc Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme
    Judith M White
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    Crit Rev Biochem Mol Biol 43:189-219. 2008
    ..In addition, there are at least four distinct mechanisms by which viral fusion proteins can be triggered to undergo fusion-inducing conformational changes...
  3. ncbi Crystal structure of glycoprotein B from herpes simplex virus 1
    Ekaterina E Heldwein
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Science 313:217-20. 2006
    ..The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes...
  4. pmc Structural basis for immunization with postfusion respiratory syncytial virus fusion F glycoprotein (RSV F) to elicit high neutralizing antibody titers
    Kurt A Swanson
    Novartis Vaccines and Diagnostics, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 108:9619-24. 2011
    ..This unanticipated structural feature explains the engineered RSV F antigen's efficiency as an immunogen. This work illustrates how structural-based antigen design can guide the rational optimization of candidate vaccine antigens...
  5. pmc Antigenic and genetic variability of human metapneumoviruses
    Bernadette G van den Hoogen
    Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Emerg Infect Dis 10:658-66. 2004
    ..Each serotype could be divided into two genetic lineages, but these did not reflect major antigenic differences...
  6. pmc The primed ebolavirus glycoprotein (19-kilodalton GP1,2): sequence and residues critical for host cell binding
    Derek Dube
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    J Virol 83:2883-91. 2009
    ....
  7. pmc Class III viral membrane fusion proteins
    Marija Backovic
    Department of Virology, Pasteur Institute, 25 rue du Dr Roux, Paris, France
    Curr Opin Struct Biol 19:189-96. 2009
    ..Here we review the newly identified group of class III viral fusion proteins, whose members include fusion proteins from rhabdoviruses, herpesviruses, and baculoviruses...
  8. ncbi Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation
    Hsien Sheng Yin
    Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nature 439:38-44. 2006
    ..The positions and structural transitions of key parts of the fusion machinery, including the hydrophobic fusion peptide and two helical heptad repeat regions, clarify the mechanism of membrane fusion mediated by the F protein...
  9. ncbi Respiratory syncytial virus with the fusion protein as its only viral glycoprotein is less dependent on cellular glycosaminoglycans for attachment than complete virus
    Sunee Techaarpornkul
    Department of Biopharmacy, Silpakorn University, Sanamchan Palace Campus, Nakornpathom, 73000, Thailand
    Virology 294:296-304. 2002
    ..This alternative pathway presumably is mediated by the sole remaining viral surface protein, F, although the formal possibility exists that some other virion-associated protein is involved...
  10. ncbi Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus
    E A Kurt-Jones
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Nat Immunol 1:398-401. 2000
    ..RSV persisted longer in the lungs of infected TLR4-deficient mice compared to normal mice. Thus, a common receptor activation pathway can initiate innate immune responses to both bacterial and viral pathogens...
  11. ncbi Role of fusion protein cleavage site in the virulence of Newcastle disease virus
    Aruna Panda
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA
    Microb Pathog 36:1-10. 2004
    ....
  12. ncbi Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
    Olav S de Leeuw
    Wageningen University and Research Centre, Animal Sciences Group, Division of Infectious Diseases, 8200 AB Lelystad, The Netherlands
    J Gen Virol 86:1759-69. 2005
    ..The effect of the HN protein on virulence was most prominent after intravenous inoculation. Interestingly, both the stem region and the globular head of the HN protein seem to be involved in determining virulence...
  13. pmc Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence
    B P Peeters
    Department of Avian Virology, Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands
    J Virol 73:5001-9. 1999
    ..These results demonstrate that genetically modified NDV can be recovered from cloned cDNA and confirm the supposition that cleavage of the F0 protein is a key determinant in virulence of NDV...
  14. pmc Requirements for budding of paramyxovirus simian virus 5 virus-like particles
    Anthony P Schmitt
    Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois 60208 3500, USA
    J Virol 76:3952-64. 2002
    ..Taken together, these data suggest that multiple viral components, including assembled nucleocapsids, have important roles in the paramyxovirus budding process...
  15. pmc Assembly and immunological properties of Newcastle disease virus-like particles containing the respiratory syncytial virus F and G proteins
    Lori W McGinnes
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Room S5 250, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 85:366-77. 2011
    ..These results suggest that these VLPs are effective RSV vaccines in mice, in contrast to other nonreplicating RSV vaccine candidates...
  16. pmc The fusion glycoprotein of human respiratory syncytial virus facilitates virus attachment and infectivity via an interaction with cellular heparan sulfate
    S A Feldman
    Laboratory of Pediatric and Respiratory Virus Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852 1448, USA
    J Virol 74:6442-7. 2000
    ..Taken together, these data suggest that RSV-F independently interacts with heparin/heparan sulfate and this type of interaction facilitates virus attachment and infectivity...
  17. pmc Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 171:111-20. 2005
    ..infect cells through low pH-dependent membrane fusion reactions mediated by their structurally similar viral fusion proteins. During fusion, these class II viral fusion proteins trimerize and refold to form hairpin-like structures, ..
  18. pmc Respiratory syncytial virus (RSV) fusion protein subunit F2, not attachment protein G, determines the specificity of RSV infection
    Jörg Schlender
    Max von Pettenkofer Institute and Gene Center, Ludwig Maximilians University Munich, D 81377 Munich, Germany
    J Virol 77:4609-16. 2003
    ..The identification of the F2 subunit as the determinant of RSV host cell specificity facilitates identification of virus receptors and should allow for development of reagents specifically interfering with RSV entry...
  19. pmc Structure of the Newcastle disease virus F protein in the post-fusion conformation
    Kurt Swanson
    Howard Hughes Medical Institute, USA
    Virology 402:372-9. 2010
    ..Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity...
  20. pmc Soluble respiratory syncytial virus fusion protein in the fully cleaved, pretriggered state is triggered by exposure to low-molarity buffer
    Supranee Chaiwatpongsakorn
    The Research Institute at Nationwide Children s Hospital, 700 Children s Dr, Columbus, OH 43205, USA
    J Virol 85:3968-77. 2011
    ..Mutation of the furin cleavage site adjacent to the fusion peptide prevented liposome association, further confirming that association is via the fusion peptide...
  21. pmc Refolding of a paramyxovirus F protein from prefusion to postfusion conformations observed by liposome binding and electron microscopy
    Sarah A Connolly
    Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208 3500, USA
    Proc Natl Acad Sci U S A 103:17903-8. 2006
    ..The reactivity of the F protein with conformation-specific mAbs and peptide binding suggest that soluble F-GCNt and membrane-bound F proteins refold through a comparable pathway...
  22. pmc Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein
    Hsien Sheng Yin
    Department of Biochemistry, Molecular Biology, and Cell Biology, Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208 3500, USA
    Proc Natl Acad Sci U S A 102:9288-93. 2005
    Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity...
  23. pmc Viral entry mechanisms: the increasing diversity of paramyxovirus entry
    Everett C Smith
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536 0509, USA
    FEBS J 276:7217-27. 2009
    ....
  24. pmc Nipah virus: vaccination and passive protection studies in a hamster model
    V Guillaume
    INSERM Unite 404 UBIVE, Institut Pasteur, CERVI, IFR 128, Lyon, France
    J Virol 78:834-40. 2004
    ....
  25. pmc Human metapneumovirus (HMPV) binding and infection are mediated by interactions between the HMPV fusion protein and heparan sulfate
    Andres Chang
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, USA
    J Virol 86:3230-43. 2012
    ....
  26. pmc Characterization of human metapneumovirus F protein-promoted membrane fusion: critical roles for proteolytic processing and low pH
    Rachel M Schowalter
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Biomedical Biological Sciences Research Building, 741 South Limestone, Lexington, KY 40536 0509, USA
    J Virol 80:10931-41. 2006
    ..Mutations in the cleavage site designed to facilitate endogenous cleavage did so with low efficiency, and our data suggest that all three N-glycosylation sites are utilized and that each affects cleavage and fusion to various degrees...
  27. ncbi The postfusion structure of baculovirus gp64 supports a unified view of viral fusion machines
    Jan Kadlec
    Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Nat Struct Mol Biol 15:1024-30. 2008
    b>Viral fusion proteins mediate the merger of host and viral membranes during cell entry for all enveloped viruses...
  28. ncbi Paramyxovirus membrane fusion: lessons from the F and HN atomic structures
    Robert A Lamb
    Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208 3500, USA
    Virology 344:30-7. 2006
    ..In the last 5 years, atomic structures of paramyxovirus F and HN proteins have been reported. The knowledge gained from these structures towards understanding the mechanism of viral membrane fusion is described...
  29. pmc Role of matrix and fusion proteins in budding of Sendai virus
    T Takimoto
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    J Virol 75:11384-91. 2001
    ..Our results indicate that both M and F proteins are able to drive the budding of SV and propose the possible role of actin in the budding process...
  30. pmc Targeted cell entry of lentiviral vectors
    Sabrina Funke
    1Division of Medical Biotechnology, Paul Ehrlich Institut, Langen, Germany
    Mol Ther 16:1427-36. 2008
    ..Because MV enters cells through direct fusion at the cell membrane, this novel targeting system should be widely applicable...
  31. pmc Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice
    Fu Shi Quan
    Zetra Biologicals LLC
    J Infect Dis 204:987-95. 2011
    ..Despite decades of research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches are therefore urgently needed to develop effective RSV vaccines...
  32. pmc The S4 genome segment of baboon reovirus is bicistronic and encodes a novel fusion-associated small transmembrane protein
    Sandra Dawe
    Department of Microbiology and Immunology, Tupper Medical Building, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Virol 76:2131-40. 2002
    ..The unique structural properties of p15 suggest that this protein is a novel member of the new family of FAST proteins...
  33. pmc Fusion activation by a headless parainfluenza virus 5 hemagglutinin-neuraminidase stalk suggests a modular mechanism for triggering
    Sayantan Bose
    Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 109:E2625-34. 2012
    ....
  34. ncbi Proteolytic activation of respiratory syncytial virus fusion protein. Cleavage at two furin consensus sequences
    G Zimmer
    , , , D-30559 Hannover, Germany
    J Biol Chem 276:31642-50. 2001
    ..Our findings indicate that both furin consensus sequences have to be cleaved in order to activate the fusion protein...
  35. pmc The cysteine-rich region and secreted form of the attachment G glycoprotein of respiratory syncytial virus enhance the cytotoxic T-lymphocyte response despite lacking major histocompatibility complex class I-restricted epitopes
    Alexander Bukreyev
    National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA
    J Virol 80:5854-61. 2006
    ..These findings reveal a novel function for the GCRR with potential implications for the generation of protective cellular responses and vaccine development...
  36. pmc Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins
    C Berlioz-Torrent
    CJF 97 03 INSERM, Interactions Moléculaires, Hôte Pathogène, Institut Cochin de Genetique Moleculaire, 75014 Paris, France
    J Virol 73:1350-61. 1999
    ....
  37. pmc Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein
    Margarita Magro
    Laboratorio de Biología Viral, Centro Nacional de Microbiología and CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain
    J Virol 84:7970-82. 2010
    ..In contrast, the peptide F478-516 or BMS-433771 must bind to F protein intermediates generated during virus-cell membrane fusion, blocking further development of this process...
  38. ncbi Structure and function of a paramyxovirus fusion protein
    Trudy G Morrison
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Biochim Biophys Acta 1614:73-84. 2003
    ..Both proteolytic cleavage of the F protein and interactions with the attachment protein are required for fusion activation in most systems. Subsequent steps in membrane merger directed by F protein are poorly understood...
  39. pmc Importance of the cytoplasmic tails of the measles virus glycoproteins for fusogenic activity and the generation of recombinant measles viruses
    Markus Moll
    Institute of Virology, Philipps University of Marburg, Marburg, Germany
    J Virol 76:7174-86. 2002
    ..Thus, glycoprotein truncation does not interfere with the successful generation of recombinant MV if fusion competence is maintained...
  40. ncbi Two novel fusion inhibitors of human respiratory syncytial virus
    Anna Lundin
    Department of Clinical Virology, University of Gothenburg, Goteborg, Sweden
    Antiviral Res 88:317-24. 2010
    ....
  41. pmc Protective efficacy and immunogenicity of an adenoviral vector vaccine encoding the codon-optimized F protein of respiratory syncytial virus
    Rebekka Kohlmann
    Department of Molecular and Medical Virology, Ruhr Universitaet Bochum, Bochum, Germany
    J Virol 83:12601-10. 2009
    ..AdV-F and AdV-Fsol induced similar levels of immunogenicity and protective efficacy. Therefore, these results encourage further development of AdV vaccines against RSV infection in humans...
  42. pmc The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins
    Reagan G Cox
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Virol 86:12148-60. 2012
    ..These results suggest a stepwise mechanism of HMPV entry mediated by the F protein through its interactions with cellular receptors, including RGD-binding integrins...
  43. pmc The paramyxovirus fusion protein C-terminal region: mutagenesis indicates an indivisible protein unit
    Aarohi Zokarkar
    Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA
    J Virol 86:2600-9. 2012
    ..Alanine scanning mutagenesis of MPER indicates that it has a regulatory role in fusion since both hyperfusogenic and hypofusogenic mutations were found...
  44. pmc C-terminal tyrosine residues modulate the fusion activity of the Hendra virus fusion protein
    Andreea Popa
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky 40536, United States
    Biochemistry 50:945-52. 2011
    ..In addition, our results implicate C-terminal tyrosine residues in the modulation of the membrane fusion reaction promoted by these viral glycoproteins...
  45. pmc The pre-transmembrane domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein is critical for membrane fusion and virus infectivity
    Zhaofei Li
    Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY 14853 1801, USA
    J Virol 83:10993-1004. 2009
    ..These studies suggested that several conserved residues (T463, G460, G462, and G474) were not required for membrane fusion but were important for budding and viral infectivity...
  46. pmc Structural basis of respiratory syncytial virus neutralization by motavizumab
    Jason S McLellan
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 17:248-50. 2010
    ..Modeling suggests that motavizumab recognizes a different quaternary configuration of the F glycoprotein than that observed in a homologous structure...
  47. pmc Identification of a GP64 subdomain involved in receptor binding by budded virions of the baculovirus Autographica californica multicapsid nucleopolyhedrovirus
    Jian Zhou
    Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY 14853, USA
    J Virol 82:4449-60. 2008
    ..Thus, the amino acid region from positions 21 to 159 was identified as a putative receptor binding domain, and amino acids 153 and 156 appear to be important for receptor binding...
  48. pmc Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus
    Nancy D Ulbrandt
    MedImmune, Inc 1 MedImmune Way, Gaithersburg, MD 20878, USA
    J Gen Virol 89:3113-8. 2008
    ..This suggests that these homologous regions play important, conserved functions in both viruses...
  49. pmc Distinct structural rearrangements of the VSV glycoprotein drive membrane fusion
    Sonia Libersou
    Centre de Recherche de Gif, Laboratoire de Virologie Moleculaire et Structurale, CNRS UMR 2472, INRA UMR 1153, IFR115, 91198 Gif sur Yvette, France
    J Cell Biol 191:199-210. 2010
    ..We suggest that the formation of these arrays, which have been shown to be an intrinsic property of the G ectodomain, induces membrane constraints, achieving the fusion reaction...
  50. pmc Pathotypical characterization and molecular epidemiology of Newcastle disease virus isolates from different hosts in China from 1996 to 2005
    Zhuo ming Qin
    Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250100, China
    J Clin Microbiol 46:601-11. 2008
    ....
  51. pmc Analysis of residues near the fusion peptide in the influenza hemagglutinin structure for roles in triggering membrane fusion
    Sudha Thoennes
    Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Virology 370:403-14. 2008
    ..3 relative to WT. The results are discussed in relation to possible mechanisms by which HA structural rearrangements are initiated at low pH and clade-specific differences near the fusion peptide...
  52. pmc West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody
    Bärbel Kaufmann
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Proc Natl Acad Sci U S A 103:12400-4. 2006
    ..The structure suggests that the E16 antibody neutralizes WNV by blocking the initial rearrangement of the E glycoprotein before fusion with a cellular membrane...
  53. pmc Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion
    Toru Takimoto
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    J Virol 76:13028-33. 2002
    ....
  54. pmc Measles virus matrix protein specifies apical virus release and glycoprotein sorting in epithelial cells
    H Y Naim
    University of Zurich, Institute of Molecular Biology, Winterthurerstrasse 190, 8057 Zurich
    EMBO J 19:3576-85. 2000
    ..Whereas this may allow virus airway shedding, the intrinsic sorting of the glycoproteins to the basolateral surface may account for systemic host infection by allowing efficient cell-cell fusion...
  55. ncbi Cell entry of enveloped viruses
    Francois Loic Cosset
    Universite de Lyon, UCB Lyon1, IFR128, Lyon, France
    Adv Genet 73:121-83. 2011
    ..These pseudoparticles are suitable for high-throughput screenings that help in the development of natural or artificial inhibitors as new therapeutics of the class of entry inhibitors...
  56. ncbi Class II virus membrane fusion proteins
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Virology 344:38-47. 2006
    ..Inhibition of the fusion protein refolding reaction confirms its importance in fusion and suggests new antiviral strategies for these medically important viruses...
  57. pmc Identification of specific histidines as pH sensors in flavivirus membrane fusion
    Richard Fritz
    Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria
    J Cell Biol 183:353-61. 2008
    ..Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion...
  58. pmc Vaccination to induce antibodies blocking the CX3C-CX3CR1 interaction of respiratory syncytial virus G protein reduces pulmonary inflammation and virus replication in mice
    Wenliang Zhang
    College of Veterinary Medicine, Department of Infectious Disease, 111 Carlton Street, University of Georgia, Athens, Georgia 30602, USA
    J Virol 84:1148-57. 2010
    ..The results suggest that RSV vaccines that induce antibodies that block G protein CX3C-CX3CR1 interaction may offer a new, safe, and efficacious RSV vaccine strategy...
  59. pmc Single-particle kinetics of influenza virus membrane fusion
    Daniel L Floyd
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:15382-7. 2008
    ..We interpret the series of intermediates preceding hemifusion as a result of the requirement that multiple copies of the trimeric hemagglutinin fusion protein be activated to initiate the fusion process...
  60. pmc New insights for development of a safe and protective RSV vaccine
    Jorge C G Blanco
    Virion Systems Inc, Rockville, MD, USA
    Hum Vaccin 6:482-92. 2010
    ..A safe and efficacious vaccine for RSV will require "re-education" of the host immune response against RSV to prevent vaccine-enhanced or severe RSV disease...
  61. ncbi A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse genetics
    Shunlin Hu
    Animal Infectious Disease Laboratory, Yangzhou University, Yangzhou, Jiangsu 225009, China
    Vaccine 27:904-10. 2009
    ....
  62. pmc Amino acid substitutions in the F-specific domain in the stalk of the newcastle disease virus HN protein modulate fusion and interfere with its interaction with the F protein
    Vanessa R Melanson
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655 0122, USA
    J Virol 78:13053-61. 2004
    ..These findings indicate that the intervening region is critical to the role of HN in the promotion of fusion and may be directly involved in its interaction with the homologous F protein...
  63. pmc Bimolecular complementation of paramyxovirus fusion and hemagglutinin-neuraminidase proteins enhances fusion: implications for the mechanism of fusion triggering
    Sarah A Connolly
    Howard Hughes Medical Institute
    J Virol 83:10857-68. 2009
    ..Like other class I viral fusion proteins, F is expressed as a prefusion metastable protein that undergoes a refolding event to induce fusion...
  64. pmc Cathepsin L is involved in proteolytic processing of the Hendra virus fusion protein
    Cara Theresia Pager
    Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, Lexington, 40536 0509, USA
    J Virol 79:12714-20. 2005
    ..These studies introduce a novel mechanism for primary proteolytic processing of viral glycoproteins and also suggest a previously unreported biological role for cathepsin L...
  65. ncbi Structure of the immature dengue virus at low pH primes proteolytic maturation
    I Mei Yu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 319:1834-7. 2008
    ..These results suggest a mechanism by which flaviviruses are processed and stabilized in the host cell secretory pathway...
  66. pmc Blue native PAGE and biomolecular complementation reveal a tetrameric or higher-order oligomer organization of the physiological measles virus attachment protein H
    Melinda A Brindley
    Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Virol 84:12174-84. 2010
    ..Results are interpreted in light of a model in which receptor binding may affect the oligomeric organization of the attachment protein complex...
  67. pmc Mumps virus matrix, fusion, and nucleocapsid proteins cooperate for efficient production of virus-like particles
    Ming Li
    Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, Pennsylvania 16802, USA
    J Virol 83:7261-72. 2009
    ..Together, these results demonstrate that different MuV structural proteins cooperate together for efficient particle production and that particle budding likely involves host class E protein machinery...
  68. ncbi A single amino acid change, Q114R, in the cleavage-site sequence of Newcastle disease virus fusion protein attenuates viral replication and pathogenicity
    Sweety Samal
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
    J Gen Virol 92:2333-8. 2011
    ..The pathogenicity was further reduced when the isoleucine at position 118 was substituted for valine...
  69. pmc Extensive syncytium formation mediated by the reovirus FAST proteins triggers apoptosis-induced membrane instability
    Jayme Salsman
    Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Virol 79:8090-100. 2005
    ....
  70. ncbi Sequence variation in the Newcastle disease virus genome
    Jacqueline A Kattenbelt
    CSIRO Livestock Industries, Australian Animal Health Laboratory, P O Bag 24, Geelong, Victoria 3220, Australia
    Virus Res 116:168-84. 2006
    ....
  71. ncbi Crystal structure of the low-pH form of the vesicular stomatitis virus glycoprotein G
    Stephane Roche
    CNRS, Unité Mixte de Recherche UMR 2472, Institut Fédératif de Recherche IFR 115, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France
    Science 313:187-91. 2006
    ..The structure provides a framework for understanding the reversibility of the G conformational change. Unexpectedly, G is homologous to gB of herpesviruses, which raises important questions on viral evolution...
  72. ncbi A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes
    Marielle Cavrois
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141 9100, USA
    Nat Biotechnol 20:1151-4. 2002
    ..BlaM cleaves the beta-lactam ring in CCF2, changing its fluorescence emission spectrum from green (520 nm) to blue (447 nm) and thereby allowing fusion to be detected by fluorescence microscopy, flow cytometry, or UV photometry...
  73. ncbi Evolutionary dynamics of Newcastle disease virus
    Patti J Miller
    Southeast Poultry Research Laboratories, USDA ARS, Southeast Poultry Research Laboratory, Athens, GA 30605, USA
    Virology 391:64-72. 2009
    ....
  74. ncbi Composition and functions of the influenza fusion peptide
    Karen J Cross
    Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Protein Pept Lett 16:766-78. 2009
    ..The role of the fusion peptide for both priming the neutral pH structure and interacting with cellular membranes during the fusion process is discussed...
  75. pmc Mapping the structure and function of the E1 and E2 glycoproteins in alphaviruses
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Structure 14:63-73. 2006
    ..The long E2 monomers, containing the cell receptor recognition motif at their extremities, are shown to rotate by about 180 degrees and to move away from the center of the spikes during fusion...
  76. pmc Membrane fusion machines of paramyxoviruses: capture of intermediates of fusion
    C J Russell
    Howard Hughes Medical Institute and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2153 N. Campus Drive, Evanston, IL 60208-3500, USA
    EMBO J 20:4024-34. 2001
    ..Peptides derived from heptad repeat regions adjacent to the fusion peptide and transmembrane domains of many viral fusion proteins form stable helical bundles and inhibit fusion specifically...
  77. ncbi Emergence of a new genetic lineage of Newcastle disease virus in West and Central Africa--implications for diagnosis and control
    G Cattoli
    OIE FAO Reference Laboratory for avian influenza and Newcastle disease, Istituto Zooprofilattico Sperimentale delle Venezie, Virology Department, Legnaro PD, Italy
    Vet Microbiol 142:168-76. 2010
    ..The present investigation provides important information on the epidemiology, diagnosis and control of NDV in Africa and highlights the importance of supporting surveillance in developing countries for transboundary animal diseases...
  78. ncbi Single mucosal immunization of recombinant adenovirus-based vaccine expressing F1 protein fragment induces protective mucosal immunity against respiratory syncytial virus infection
    Sol Kim
    Division of Life and Pharmaceutical Sciences, and the Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, 11 1 Dae Hyun Dong, Seo Dae Mun Gu, Seoul 120 750, Republic of Korea
    Vaccine 28:3801-8. 2010
    ..Taken together, our data demonstrate that a single intranasal administration of rAd/F1co is sufficient for the protection and represents a promising prophylactic vaccination regimen against RSV infection...
  79. pmc Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan
    L Yu
    Animal Health Biotechnology Laboratory, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604
    J Clin Microbiol 39:3512-9. 2001
    ..Finally, a cross-protective experiment indicated that at least one strain (Ch-A7/96) among our NDV isolates was an antigenic variant, responsible for recent outbreaks of ND in vaccinated chicken flocks...
  80. ncbi Therapeutic monoclonal antibody treatment targeting respiratory syncytial virus (RSV) G protein mediates viral clearance and reduces the pathogenesis of RSV infection in BALB/c mice
    Lia M Haynes
    National Centers for Immunization and Respiratory Diseases, Div of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch, 1600 Clifton Rd NE, Mailstop G 18, Atlanta, GA 30333, USA
    J Infect Dis 200:439-47. 2009
    ....
  81. pmc Characterization of EBV gB indicates properties of both class I and class II viral fusion proteins
    Marija Backovic
    Department of Biochemistry, Molecular Biology, Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Virology 368:102-13. 2007
    ..of the gB constructs formed rosette structures reminiscent of the postfusion aggregates formed by other viral fusion proteins. However, substitution of putative fusion loop residues, WY(112-113) and WLIY(193-196), with less ..
  82. ncbi Sequence analysis of the fusion protein gene from infectious salmon anemia virus isolates: evidence of recombination and reassortment
    M Devold
    University of Bergen, Department of Biology, N 5020 Bergen, Norway
    J Gen Virol 87:2031-40. 2006
    ..This shows that recombination is one of the evolutionary mechanisms shaping the genome of ISAV. The possible importance of the INs with respect to virulence remains uncertain...
  83. pmc Henipavirus mediated membrane fusion, virus entry and targeted therapeutics
    Deborah L Steffen
    Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD 20814, USA
    Viruses 4:280-308. 2012
    ..Finally, recent candidate therapeutics for henipavirus-mediated disease are summarized in light of their ability to inhibit HeV and NiV entry by targeting their G and F glycoproteins...
  84. pmc Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention
    Margarita Magro
    Biología Viral, Centro Nacional de Microbiología and CIBER de Enfermedades Respiratorias, 28220 Madrid, Spain
    Proc Natl Acad Sci U S A 109:3089-94. 2012
    ..These antibodies therefore offer unique possibilities for immune intervention against hRSV, and their production should be assessed in trials of hRSV vaccines...
  85. pmc Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodalton fusion protein
    J F Rodriguez
    Department of Biochemistry, State University of New York Health Science Center at Brooklyn, New York 11203 2098
    J Virol 61:3550-4. 1987
    ..Inspection of the deduced amino acid sequence of the 14-kilodalton protein revealed consensus sites with the hemagglutinin precursor of influenza A virus and with adenylate kinase and cytochrome c of various species...
  86. pmc Respiratory syncytial virus-neutralizing monoclonal antibodies motavizumab and palivizumab inhibit fusion
    Kelly Huang
    MedImmune, One MedImmune Way, Gaithersburg, MD 20878, USA
    J Virol 84:8132-40. 2010
    ..Based on models developed from numerous studies of viral fusion proteins, our results indicate that these antibodies may prevent conformational changes in F protein required for ..
  87. pmc Integrin alphavbeta1 promotes infection by human metapneumovirus
    Gabriella Cseke
    Department of Chemistry, Vanderbilt University College of Arts and Sciences, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 106:1566-71. 2009
    ..Recombinant hMPV F protein binds to cells, whereas Arg-Gly-Glu (RGE)-mutant F protein does not. These data suggest that alphavbeta1 integrin is a functional receptor for hMPV...
  88. pmc Structural characterization of the fusion-active complex of severe acute respiratory syndrome (SARS) coronavirus
    Paolo Ingallinella
    Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 600, 00040 Pomezia, Italy
    Proc Natl Acad Sci U S A 101:8709-14. 2004
    ..Inhibitors binding to HR regions of fusion proteins have been shown to be efficacious against many viruses, notably HIV. Our results may help in the design of anti-SARS therapeutics...
  89. ncbi Design and characterization of human respiratory syncytial virus entry inhibitors
    Ling Ni
    Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    Antivir Ther 10:833-40. 2005
    ..Our results suggest that these rationally designed protein inhibitors could serve as a new class of anti-hRSV agents...
  90. pmc Isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo
    Nancy D Ulbrandt
    MedImmune, Inc, 1 MedImmune Way, Gaithersburg, MD 20878, USA
    J Virol 80:7799-806. 2006
    ..The data suggest that a monoclonal antibody could be used prophylactically to prevent lower respiratory tract disease caused by hMPV...
  91. pmc Roles for the cytoplasmic tails of the fusion and hemagglutinin-neuraminidase proteins in budding of the paramyxovirus simian virus 5
    David L Waning
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    J Virol 76:9284-97. 2002
    ..P. Schmitt, G. P. Leser, D. L. Waning, and R. A. Lamb, J. Virol. 76:3953-3964, 2002) suggest a redundant role for the cytoplasmic tails of the HN and F proteins in virus assembly and budding...
  92. pmc A fluorescence polarization assay using an engineered human respiratory syncytial virus F protein as a direct screening platform
    Minyoung Park
    Department of Chemical and Systems Biology, Stanford University School of Medicine, CA 94305, USA
    Anal Biochem 409:195-201. 2011
    ..We anticipate that this new 5HB system will provide an effective tool to identify and study potential antivirals to control hRSV infection...
  93. ncbi RFI-641 inhibits entry of respiratory syncytial virus via interactions with fusion protein
    V Razinkov
    Department of Biological Chemistry, Wyeth-Ayerst Research, Pearl River, NY 10965, USA
    Chem Biol 8:645-59. 2001
    ..The results described here demonstrate that a non-peptidic, small molecule can inhibit binding and fusion of enveloped virus specifically via interaction with the viral fusion protein...
  94. pmc Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 82:8891-5. 2008
    ..Only 6% of all HMPV lineage A F sequences have 294G, and none of the lineage B sequences have 294G. Thus, acidic pH is not a general trigger of HMPV F proteins for activity...
  95. pmc Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years
    Chin Fen Yang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA
    Virol J 6:138. 2009
    ..Human metapneumovirus (HMPV) is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion) gene sequences collected over a 20-year period...
  96. pmc Prophylactic treatment with a G glycoprotein monoclonal antibody reduces pulmonary inflammation in respiratory syncytial virus (RSV)-challenged naive and formalin-inactivated RSV-immunized BALB/c mice
    Gertrud U Radu
    Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G 18, Atlanta, GA 30333, USA
    J Virol 84:9632-6. 2010
    ..It is possible that antibodies with similar reactivities might prevent enhanced disease and improve the safety of nonlive virus vaccines...
  97. pmc Residues of the human metapneumovirus fusion (F) protein critical for its strain-related fusion phenotype: implications for the virus replication cycle
    Vicente Mas
    Biología Viral, Centro Nacional de Microbiología and CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
    J Virol 85:12650-61. 2011
    ..J. Virol. 83:1511-1522, 2009) highlight the relevance of certain residues in the linker region and domain II of the pretriggered hMPV F protein for the process of membrane fusion...
  98. pmc Functional interaction between paramyxovirus fusion and attachment proteins
    Jin K Lee
    Department of Pediatrics, Emory University School of Medicine, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 283:16561-72. 2008
    ..Their localization in structural models of F and H suggests that placement in particular of F residue 233 in close proximity to the 110-114 region of H is structurally conceivable...
  99. pmc Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion
    L Gonzalez-Reyes
    Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    Proc Natl Acad Sci U S A 98:9859-64. 2001
    ..Both cleavages are required for the F protein to be active in membrane fusion as judged by syncytia formation, and they allow changes in F structure from cone- to lollipop-shaped spikes and the formation of rosettes by anchorless F...
  100. ncbi Genetic variability of the fusion protein and circulation patterns of genotypes of the respiratory syncytial virus
    Yun Kyung Kim
    Department of Pediatrics, Korea University College of Medicine, Seoul, Korea
    J Med Virol 79:820-8. 2007
    ..Our results indicated that the F gene of the RSVs may be continuously evolving under selective pressure in a distinct pattern, and that the genetic variability of the F protein is associated with that of the G protein...
  101. ncbi Class III viral membrane fusion proteins
    Marija Backovic
    Department of Virology, Pasteur Institute, 75015, Paris, France
    Adv Exp Med Biol 714:91-101. 2011
    Members of class III of viral fusion proteins share common structural features and molecular architecture, although they belong to evolutionary distant viruses and carry no sequence homology...

Research Grants62

  1. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010
    ..This research aims to illustrate the structural details of this process driven by the Sindbis viral fusion proteins, and provides a general approach for structural investigation of membrane fusion carried out by the ..
  2. Intra- and Inter-Neuronal Viral Trafficking
    Glenn F Rall; Fiscal Year: 2012
    ..Successful completion of the proposed aims will provide the foundation for the development of treatments to prevent or reverse the life-threatening consequences of chronic CNS infections caused by viruses. ..
  3. ELEXSYS E500 CW EPR SPECTROMETER
    Jack H Freed; Fiscal Year: 2010
    ..requiring this spectrometer will investigate protein-membrane interaction of membrane-modifying and viral fusion proteins, as well as Parkinson's disease implicated proteins, and other studies...
  4. Molecular Regulation of Fusion: Voltage Dependence and Local Physical Interaction
    Fredric S Cohen; Fiscal Year: 2013
    ..Methods to monitor membrane insertion of segments of viral fusion proteins will be developed through coupling lipophilic, charged probes to a fusion protein and ..
  5. Hematopoietic Stem Cell Differentiation and Commitment
    Margaret Goodell; Fiscal Year: 2010
    ..in the absence of bone marrow transplantation, b) Increasing the efficiency of fusion using inducible viral fusion proteins, and c) Studying the nuclear reprogramming events occurring upon fusion using BAC-transgenics expressing ..
  6. Solid-State NMR of Viral Fusion Peptides and Proteins
    David P Weliky; Fiscal Year: 2013
    ..the viruses cause major human diseases and because the proteins serve as prototypes for other class I viral fusion proteins. There is particular emphasis on the N-terminal "fusion peptide" (FP) domains of the fusion proteins, ..
  7. Study of the structure, function and regulation of gB, the fusion protein of HSV
    Roselyn J Eisenberg; Fiscal Year: 2013
    ..However, gH/gL bears no structural similarities to viral fusion proteins. Moreover, gH/gL and gB can effect fusion when the two proteins are in separate cells (in trans)...
  8. Structures and lipid interactions of curvature-inducing membrane peptides by NMR
    Mei Hong; Fiscal Year: 2013
    ..or cross the membrane into cells by forming permanent or transient pores, and hydrophobic domains of viral fusion proteins that merge the virus envelope and the target cell membrane to cause viral entry...
  9. High Resolution Structure of the HIV gp41 Protein
    Kelly Sackett; Fiscal Year: 2010
    ..gp41 fusion protein has broad implications by detailing a potential therapeutic target to inhibit fusion and therefore infection, and by providing functional insights into the mechanism by which viral fusion proteins merge membranes.
  10. BIOPHYSICS OF INFLUENZA HEMAGGLUTININ-MEDIATED FUSION
    FREDRIC COHEN; Fiscal Year: 2001
    ..As HA and many other viral fusion proteins, including that of HIV-1, have the same core structure, and all viral fusion proteins initiate their ..
  11. Regulation of Viral Membrane Fusion
    Richard Compans; Fiscal Year: 2003
    ..Although major advances have been made in our knowledge of the structure of viral fusion proteins, many aspects of the fusion process remain unknown...
  12. TRANSMEMBRANE DOMAINS IN MEMBRANE FUSION
    John Lenard; Fiscal Year: 2003
    ..4) The TMD sequence requirements for fusion will be determined for those viral fusion proteins that lack gly residues...
  13. HIV ENV TRANSMEMBRANE DOMAIN AND FUSION
    RANDALL OWENS; Fiscal Year: 1999
    ..in this area of antiviral research has been slow, primarily because the precise mechanism by which viral fusion proteins function has not been determined...
  14. Inhibition of the Membrane Fusion Proteins of Flaviviruses and Alphaviruses
    Margaret Kielian; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  15. Recombinant antigen assays for Lassa and other arenaviru
    Robert Garry; Fiscal Year: 2005
    ....
  16. High Throughput Screening-Based Identification of Measles Virus Probes
    RICHARD PLEMPER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  17. Structure-Based Design of Nipah Virus Entry Inhibitors
    RICHARD PLEMPER; Fiscal Year: 2006
    ..These will be characterized for their activity against live Nipah (aim 3). ..
  18. HIV-1 Tat modulation of HCV replication and pathogenesis
    Robert Garry; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  19. Rapid screen for Ebola virus membrane interactions/drugs
    Robert Garry; Fiscal Year: 2005
    ....
  20. Peptide drugs against influenza virus
    Robert Garry; Fiscal Year: 2007
    ..The studies proposed in this application will address an urgent public health and biodefense need for new drugs that are effective against influenza viruses. [unreadable] [unreadable]..
  21. Rapid screen for HIV TM membrane interactions/drugs
    Robert Garry; Fiscal Year: 2004
    ..The approaches piloted here may be useful for developing drugs against the AIDS virus and a broad range of important human viral pathogens with similar fusion proteins. ..
  22. STRUCTURE AND EXPRESSION OF THE PARAMYXOVIRUS SV5 GENOME
    Robert A Lamb; Fiscal Year: 2010
    ..This is because membrane fusion is a process central in cell biology. The class I viral fusion proteins are trapped in a meta-stable state and on activation these proteins undergo a significant re-folding event ..
  23. Immune Responses to Biodefense Vaccines Early in Life
    Marcela F Pasetti; Fiscal Year: 2010
    ..It will also provide knowledge to better understand the mechanisms mediating neonatal-infant immunity to develop more effectivevaccines to prevent diseases early in life. ..
  24. CONTROL OF PARAMYXOVIRUS RNA SYNTHESIS
    GRIFFITH PARKS; Fiscal Year: 2008
    ..Our work will increase our understanding of important virus-host cell interactions, as well as provide new approaches to improve the safety and potency of vaccine vectors. [unreadable] [unreadable] [unreadable]..
  25. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2008
    ..abstract_text> ..
  26. In Vivo Antiviral Activity of Saponins
    MICHAEL RONER; Fiscal Year: 2007
    ..The extract is well suited to such an application as it is pH stable, is already approved as a food additive, is inexpensive, readily available and is soluble in water. [unreadable] [unreadable] [unreadable]..
  27. A MODEL SYSTEM FOR PHYSIOLOGICAL EXOCYTOSIS
    FREDRIC COHEN; Fiscal Year: 2009
    ..Two classes of viral fusion proteins have been identified based on similarity in crystal structures...
  28. Macrophage defense againt M. tuberculosis
    Jennifer Wang; Fiscal Year: 2008
    ..abstract_text> ..
  29. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2010
    ..abstract_text> ..
  30. HMPV Fusion protein-host cell interactions
    REBECCA DUTCH; Fiscal Year: 2008
    ..This research will address significant questions concerning the cellular factors which interact with the HMPV fusion protein to facilitate attachment of the virus and entry into cells. [unreadable] [unreadable] [unreadable]..
  31. Pathogenesis of RSV disease in the Elderly
    Edward Walsh; Fiscal Year: 2008
    ..abstract_text> ..
  32. MOLECULAR MECHANISMS OF VIRAL MEMBRANE FUSION PROTEINS
    Judith White; Fiscal Year: 2009
    ..provided by applicant): The long term objectives of this research are to elucidate the mechanisms of viral fusion proteins and to use this information to design anti-fusion anti-virals...
  33. Baculovirus envelope proteins and insect cells
    GARY BLISSARD; Fiscal Year: 2009
    ..In addition, results of many of these studies will be directly applicable to new and exciting biotechnological applications of baculoviruses in mammalian cells. ..
  34. Vaccinia: T-cell immunity and homing to skin
    David Koelle; Fiscal Year: 2009
    ..Our data will assist in evaluating the immunogenicity of these constructs. ..
  35. Nasal carriage of S. aureus: host-pathogen interactions
    Alexander M Cole; Fiscal Year: 2010
    ..abstract_text> ..
  36. Gene delivery methods for global therapy in the CNS
    Miguel Esteves; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  37. Novel Model to Predict Safety and Efficacy of Microbicides
    Gregory Prince; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  38. Human Metapneumovirus Infections in Children
    John Williams; Fiscal Year: 2004
    ..The specific aims of this study are to define the prevalence of hMPV infection in children, describe fully the spectrum of clinical illness and identify serologic markers of seroconversion and correlates of immunity. ..
  39. Mucosal RSV vaccine using CpG oligodeoxynucleotides
    Gregory Prince; Fiscal Year: 2003
    ..Phase II activities would use that information to formulate a vaccine that would be both effective and safe. ..
  40. ADAMS--ADHESION, FUSION AND SIGNALING ACTIVITIES
    Judith White; Fiscal Year: 2004
    ..For example, the dual functions of ADAMs as both adhesion molecules and as proteases suggests that they may be key players in the de-adhesive, migratory, and re-adhesive activities involved in tumor metastasis. ..
  41. HIV-1 entry inhibitors targeting Phe43 cavity in gp120
    Asim Debnath; Fiscal Year: 2003
    ..The long-term goal of this proposal is to optimize the lead compounds through chemical synthesis and structure activity analyses and select 3-4 best compounds for further preclinical studies as novel ianti-HIV-1 chemotherapeutic agents. ..
  42. Lassa and Nipah Virus Interferon-Antagonists
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..abstract_text> ..
  43. EBOLA VIRUS INTERFERON-ANTAGONISTS AND VIRULENCE
    CHRISTOPHER BASLER; Fiscal Year: 2003
    ..VP35 and VP24 may also prove to be attractive targets for new antiviral agents. Inhibition of anti-IFN factors may sensitize Ebola virus infected cells to the antiviral effects of IFN and may also enhance the effect of antiviral agents. ..
  44. CHIMERIC RNA/DNA OLIGONUCLEOTIDE BASED GENE THERAPY
    YEONG HAU LIEN; Fiscal Year: 2003
    ..This preclinical study will provide critical information for future development of optimal gene targeting therapy for treating hereditary renal diseases, such as autosomal dominant polycystic kidney disease. ..
  45. STRUCTURE/FUNCTION OF GTC, A NOVEL GOLGI PROTEIN COMPLEX
    FREDERICK HUGHSON; Fiscal Year: 2002
    ..These studies will provide the first insights into the role of this novel protein complex in the process of intracellular protein traffic. ..
  46. STRUCTURAL ANALYSIS OF INTRACELLULAR TRAFFICKING PROTEIN
    FREDERICK HUGHSON; Fiscal Year: 2002
    ....
  47. Proteomics of Staphylococcus aureus nasal carriage
    Alexander Cole; Fiscal Year: 2003
    ....
  48. Activation of Apoptosis by a Simian Virus 5 Mutant
    GRIFFITH PARKS; Fiscal Year: 2005
    ..At the completion of this project, we will have established a firm baseline from which we can pursue detailed mechanistic questions concerning interactions of paramyxoviruses with host antiviral pathways. ..
  49. PARAMYXOVIRUS MEMBRANE FUSION
    TRUDY MORRISON; Fiscal Year: 2005
    ..4) explore the role of attachment in initiation of fusion. ..
  50. Novel Organophosphorous Hydrolases for Decontamination
    Andrew Mesecar; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  51. Immune Correlates of HSV-2 Disease Severity
    David Koelle; Fiscal Year: 2007
    ....
  52. Broad-Spectrum Therapeutic Human Antibodies for Dengue Virus Infections
    Yorgo Modis; Fiscal Year: 2006
    ..This project will provide clinicians and public health officials with new means to manage natural, accidental and intentional flavivirus outbreaks. [unreadable] [unreadable] [unreadable]..
  53. Enhancing the Potency of Viral Vectors with C3d
    GRIFFITH PARKS; Fiscal Year: 2006
    ..At the conclusion of these studies, we will have developed a powerful new class of viral vectors to elicit a more rapid and potent antigen-specific antibody response in the respiratory tract. ..
  54. Heat Shock Proteins and Helicobacter Pylori Pathogenesis
    Evelyn Kurt Jones; Fiscal Year: 2006
    ..Specifically, we will use in vitro and in vivo approaches to investigate the role of heat shock proteins in H. pylori infection and pathogenesis and development of tumors. ..
  55. Proteolytic cleavage of the Hendra virus fusion protein
    REBECCA DUTCH; Fiscal Year: 2006
    ..abstract_text> ..
  56. Rational Design of CCR5 Antagonists as Anti-HIV-1 Drugs
    Asim Debnath; Fiscal Year: 2006
    ..The long term goal is to optimize the lead compounds through chemical syntheses and structure activity relationship (SAR) analyses and select the 3-4 best compounds for further preclinical studies. ..
  57. HSV Immunotherapeutic Vaccine
    David Koelle; Fiscal Year: 2005
    ....
  58. Determinanats of Protective Immunity to Metapneumovirus
    John Williams; Fiscal Year: 2005
    ..The proposed work will provide the candidate skills and experience to be an outstanding physician-scientist in the field of human viral immunology. ..
  59. Intradermal vaccinia:T-cell specificity and skin homing
    David Koelle; Fiscal Year: 2005
    ..2) Measure E-selectin binding by vaccinia-specific CD8 cells, and 2.3) Compare CLA and E-selectin binding by vaccinia-specific T-cells in recent and distant vaccines. ..
  60. Small Molecule X-Ray Diffraction Instrumentation
    Andrew Mesecar; Fiscal Year: 2002
    ..abstract_text> ..