ccaat binding factor

Summary

Summary: A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.

Top Publications

  1. ncbi The long terminal repeat (LTR) of ERV-9 human endogenous retrovirus binds to NF-Y in the assembly of an active LTR enhancer complex NF-Y/MZF1/GATA-2
    Xiuping Yu
    Department of Biochemistry and Molecular Biology and Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 280:35184-94. 2005
  2. pmc Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters
    Carol Imbriano
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milan, Italy
    Mol Cell Biol 25:3737-51. 2005
  3. ncbi The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA
    Orna Ben-Naim
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Plant J 46:462-76. 2006
  4. ncbi CCAAT/enhancer binding protein and nuclear factor-Y regulate adiponectin gene expression in adipose tissue
    Sang Kyu Park
    Department of BiochemistryMolecular Biology, Institute of Genetic Science, Seoul, Korea
    Diabetes 53:2757-66. 2004
  5. pmc Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response
    H Yoshida
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8304, Japan
    Mol Cell Biol 21:1239-48. 2001
  6. pmc The histone-like NF-Y is a bifunctional transcription factor
    Michele Ceribelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    Mol Cell Biol 28:2047-58. 2008
  7. ncbi Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit
    Hideya Goda
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464 8601 Japan
    Arch Microbiol 184:93-100. 2005
  8. ncbi The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of histone deacetylases
    Yiwen Peng
    Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:8385-94. 2003
  9. pmc Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p
    J Blom
    Section for Molecular Biology, Swammerdam Institute of Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands
    Appl Environ Microbiol 66:1970-3. 2000
  10. ncbi DeltaNp63alpha up-regulates the Hsp70 gene in human cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Division of Endocrinology and Metabolism, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205 2196, USA
    Cancer Res 65:758-66. 2005

Research Grants

  1. Role of EZH2 in Epithelial Ovarian Cancer
    Rugang Zhang; Fiscal Year: 2013
  2. TRANSCRIPTIONAL REGULATION OF TYPE I COLLAGEN GENES BY C
    Sankar Maity; Fiscal Year: 1999
  3. ROLE OF CBF/NF-Y IN TRANSCRIPTION: TYPE I COLLAGEN GENES
    Sankar Maity; Fiscal Year: 2004
  4. CELLULAR MECHANISMS IN SYNTHESIS OF EXTRACELLULAR MATRIX
    Benoit de Crombrugghe; Fiscal Year: 2003
  5. TETRAHYDROBIOPTERIN BIOSYNTHESIS BY DOPAMINE NEURONS
    Gregory Kapatos; Fiscal Year: 2008
  6. REGULATION OF HIV2 TRANSCRIPTION AND REPLICATION
    DAVID MARKOVITZ; Fiscal Year: 2002
  7. CXC Chemokines and HIV Pathogenesis
    DAVID MARKOVITZ; Fiscal Year: 2009
  8. Sequence Determinants of Meiotic Recombination Hotspots
    Walter Steiner; Fiscal Year: 2007
  9. C-X-C Chemokines and Kaposi's Sarcoma
    DAVID MARKOVITZ; Fiscal Year: 2005
  10. MAP KINASE REGULATION OF MICROGLIAL ACTIVATION
    Narayan Bhat; Fiscal Year: 2004

Detail Information

Publications199 found, 100 shown here

  1. ncbi The long terminal repeat (LTR) of ERV-9 human endogenous retrovirus binds to NF-Y in the assembly of an active LTR enhancer complex NF-Y/MZF1/GATA-2
    Xiuping Yu
    Department of Biochemistry and Molecular Biology and Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 280:35184-94. 2005
    ....
  2. pmc Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters
    Carol Imbriano
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milan, Italy
    Mol Cell Biol 25:3737-51. 2005
    ....
  3. ncbi The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA
    Orna Ben-Naim
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Plant J 46:462-76. 2006
    ..plant-specific CCT (CO, CO-like, TIMING OF CAB EXPRESSION 1) domain of both proteins binds the trimeric CCAAT binding factor (CBF) via its HAP5/NF-YC component...
  4. ncbi CCAAT/enhancer binding protein and nuclear factor-Y regulate adiponectin gene expression in adipose tissue
    Sang Kyu Park
    Department of BiochemistryMolecular Biology, Institute of Genetic Science, Seoul, Korea
    Diabetes 53:2757-66. 2004
    ..These findings demonstrated that C/EBP and NF-Y are critical for the regulation of the adiponectin expression in response to nutrients and in the course of adipocyte differentiation...
  5. pmc Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response
    H Yoshida
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8304, Japan
    Mol Cell Biol 21:1239-48. 2001
    ..We concluded that ATF6 homo- or heterodimers recognize and bind directly to both the DNA and adjacent protein NF-Y and that this complex formation process is essential for transcriptional induction of ER chaperones...
  6. pmc The histone-like NF-Y is a bifunctional transcription factor
    Michele Ceribelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    Mol Cell Biol 28:2047-58. 2008
    ..These data indicate that NF-Y is embedded in positive as well as in negative methyl histone marks, serving a dual function in transcriptional regulation, as an activator or as a repressor...
  7. ncbi Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit
    Hideya Goda
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464 8601 Japan
    Arch Microbiol 184:93-100. 2005
    ..nidulans. The nuclear translocation of the A. oryzae complex was found to be dependent on two redundant localising signals in HapB...
  8. ncbi The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of histone deacetylases
    Yiwen Peng
    Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:8385-94. 2003
    ..In endothelial cells, however, association of HDACs, NFY, and GATA6 is interrupted potentially through endothelial cell-specific signaling/mechanism, thus favoring the balance toward acetylation and activation of the VWF promoter...
  9. pmc Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p
    J Blom
    Section for Molecular Biology, Swammerdam Institute of Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands
    Appl Environ Microbiol 66:1970-3. 2000
    ..Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms...
  10. ncbi DeltaNp63alpha up-regulates the Hsp70 gene in human cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Division of Endocrinology and Metabolism, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205 2196, USA
    Cancer Res 65:758-66. 2005
    ..In addition, DeltaNp63alpha regulates transcription of the hsp70 gene through its interaction with the CCAAT binding factor and NF-Y transcription factors which are known to form a complex with the CCAAT box located in the hsp70 ..
  11. ncbi Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
    Anna Testa
    Dipartimento di Biologia Animale, Università di Modena e Reggio, Via Campi 213 D, 41100 Modena, Italy
    J Biol Chem 280:13606-15. 2005
    ..The abundance of "unorthodox" CCAAT positions highlights an unexpected complexity of the NF-Y-mediated transcriptional network...
  12. ncbi NF-Y-dependent cyclin B2 expression in colorectal adenocarcinoma
    Seon Hwa Park
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Research Institute of Clinical Medicine, Brain Korea 21 Program for Medical Science, Chonbuk National University Medical School and Hospital, Jeonju, Jeonbuk, South Korea
    Clin Cancer Res 13:858-67. 2007
    ..This study examined the level of cyclin B2 overexpression according to the histologic findings and investigated the mechanism(s) and clinical implications of cyclin B2 overexpression in colorectal adenocarcinomas...
  13. ncbi An overview of the CCAAT-box binding factor in filamentous fungi: assembly, nuclear translocation, and transcriptional enhancement
    Masashi Kato
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    Biosci Biotechnol Biochem 69:663-72. 2005
    ..In this review, the focus is on the CCAAT-box binding protein of filamentous fungi. The assembly, nuclear translocation, and transcriptional enhancement mechanisms of the CCAAT-box binding protein are discussed...
  14. ncbi Cell cycle regulation of NF-YC nuclear localization
    Mattia Frontini
    Dipartimento di Biologia Animale Universita di Modena e Reggio Emilia, Modena, Italy
    Cell Cycle 3:217-22. 2004
    ..These data point to the control of HFM heterodimerization as an important layer of NF-Y regulation during cell cycle progression...
  15. pmc Inhibition of CBF/NF-Y mediated transcription activation arrests cells at G2/M phase and suppresses expression of genes activated at G2/M phase of the cell cycle
    Qianghua Hu
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Nucleic Acids Res 34:6272-85. 2006
    ..This study suggested that the activation domain of CBF-B plays an essential role in the transcription activation of Cyclin B1 and Aurora A genes at G2/M phase, thus regulating cell cycle progression at G2/M phase...
  16. ncbi Mutant p53 gain of function: the NF-Y connection
    Melissa J Peart
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Cancer Cell 10:173-4. 2006
    ..These data thereby implicate transcriptional activation by mutant p53 as a key mechanism responsible for its oncogenic activity...
  17. ncbi A single subunit of a heterotrimeric CCAAT-binding complex carries a nuclear localization signal: piggy back transport of the pre-assembled complex to the nucleus
    Stefan Steidl
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, D 30167 Hannover, Germany
    J Mol Biol 342:515-24. 2004
    ..This enables the cell to provide equimolar concentrations of all subunits to the nucleus...
  18. pmc A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response
    Paolo Benatti
    Dipartimento di Biologia Animale, Università di Modena e Reggio, Via Campi 213 D, 41100 Modena, Italy
    Nucleic Acids Res 36:1415-28. 2008
    ..Our data highlight the importance of fine balancing the NF-Y-p53 duo for cell survival by (i) maintaining transcription of anti-apoptotic genes and (ii) preventing p53 activation that triggers the apoptotic cascade...
  19. ncbi p53 and its homologues, p63 and p73, induce a replicative senescence through inactivation of NF-Y transcription factor
    M S Jung
    National Research Laboratory for Cell Cycle Regulation, Department of Microbiology, Dankook University College of Medicine, Chonan, 330-714, Korea
    Oncogene 20:5818-25. 2001
    ..Since NF-Y binds to many promoters besides the cdk1 and cyclin B promoters, inactivation of NF-Y by p53 family genes may be a general mechanism for transcription repression in replicative senescence...
  20. pmc The cyclin B1 gene is actively transcribed during mitosis in HeLa cells
    S Sciortino
    Dipartimento di Oncologia Sperimentale, Istituto Regina Elena, Via delle Messi d'Oro 156, 00158 Rome, Italy
    EMBO Rep 2:1018-23. 2001
    ..Consistent with this, we show that the cyclin B1 promoter is occupied and bound to NF-Y during mitosis in vivo. Our results provide the first example of RNA polymerase II-dependent transcription during mitosis in mammalian cells...
  21. ncbi Transcriptional regulation of cellular ageing by the CCAAT box-binding factor CBF/NF-Y
    Koozi Matuoka
    Department of Chemstry and Chemical Biology, Rutgers State University of New Jersey, Piscataway, 08854, USA
    Ageing Res Rev 1:639-51. 2002
    ..In fact, the activities of NF-Y and E2F decrease during ageing and a dominant negative NF-YA induces SA-gal. Based on these observations, NF-Y appears to play an important role in the process of cellular ageing...
  22. pmc Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain
    Y Xing
    Department of Biology, MIT, Cambridge, MA 02139
    EMBO J 12:4647-55. 1993
    ..These findings suggest that short regions of HAP2 and HAP3 comprise a hybrid DNA-binding domain and that this domain can help hold the two subunits together in the CCAAT-binding complex...
  23. pmc Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron
    Peter Hortschansky
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, and Friedrich Schiller University Jena, Jena, Germany
    EMBO J 26:3157-68. 2007
    ..Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae...
  24. ncbi HSP-CBF is an NF-Y-dependent coactivator of the heat shock promoters CCAAT boxes
    C Imbriano
    Dipartimento di Biologia Animale, U di Modena e Reggio, Via Campi 213 D, 41100 Modena, Italy
    J Biol Chem 276:26332-9. 2001
    ..In yeast two-hybrid assays HSP-CBF interacts with NF-YB. These data implicate HSP-CBF as a non-DNA binding coactivator of heat shock genes that act on a DNA-bound NF-Y...
  25. ncbi The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y
    Christophe Romier
    Département de Biologie et Génomique Structurales, Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM Universite Louis Pasteur, 1 rue Laurent Fries, B P 10142, 67404 Illkirch Cedex, France
    J Biol Chem 278:1336-45. 2003
    ..In particular, it emphasizes the role of the newly described alphaC helix of NF-YC, which is both important for NF-Y trimerization and a target for regulatory proteins, such as MYC and p53...
  26. ncbi Genome-wide identification and expression analysis of the NF-Y family of transcription factors in Triticum aestivum
    Troy J Stephenson
    CSIRO Plant Industry, St Lucia, QLD, Australia
    Plant Mol Biol 65:77-92. 2007
    ..Organ-specific expression and differential response to drought indicate a plant-specific biological role for various members of this transcription factor family...
  27. ncbi Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway
    Jeanho Yun
    National Research Laboratory, Department of Microbiology, Dankook University College of Medicine, Cheonan, 330 714, Korea
    J Biol Chem 278:36966-72. 2003
    ..These results facilitate the elucidation of the regulatory mechanisms of cell cycle progression involving the p21-cdk2-NF-Y signaling pathway...
  28. ncbi The CCAAT-binding complex of eukaryotes: evolution of a second NLS in the HapB subunit of the filamentous fungus Aspergillus nidulans despite functional conservation at the molecular level between yeast, A.nidulans and human
    André Tüncher
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute, Beutenbergstrasse 11a, D 07745 Jena, Germany
    J Mol Biol 352:517-33. 2005
    ..nidulans hapB mutant was complemented, at least in part, by both the human NF-YA and S.cerevisiae Hap2p this finding suggests that the piggy-back mechanism of nuclear transport found for A.nidulans is conserved in yeast and human...
  29. ncbi 17beta-estradiol (E2) induces cdc25A gene expression in breast cancer cells by genomic and non-genomic pathways
    Wan Ru Lee
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843 4466, USA
    J Cell Biochem 99:209-20. 2006
    ..Thus, both genomic and non-genomic pathways of estrogen action are involved in induction of cdc25A in breast cancer cells...
  30. ncbi Nuclear factor-Y binding to the topoisomerase IIalpha promoter is inhibited by both the p53 tumor suppressor and anticancer drugs
    Ashish A Joshi
    Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    Mol Pharmacol 63:359-67. 2003
    ..The data presented points to the existence of both p53-dependent and -independent mechanisms for regulating NF-Y binding to ICBs in the topo IIalpha promoter and thus the modulation of topo IIalpha gene expression...
  31. pmc The tetraplex (CGG)n destabilizing proteins hnRNP A2 and CBF-A enhance the in vivo translation of fragile X premutation mRNA
    Samer Khateb
    Department of Biochemistry, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Nucleic Acids Res 35:5775-88. 2007
    ..Taken together, our results suggest that secondary structures of (CGG)n in mRNA obstruct its translation and that quadruplex-disrupting proteins alleviate the translational block...
  32. ncbi Regulation of novel members of the Arabidopsis thaliana CCAAT-binding nuclear factor Y subunits
    Giuliana Gusmaroli
    Dipartimento di Genetica e Biologia dei Microrganismi, Universita di Milano, Via Celoria 26, 20133, Milan, Italy
    Gene 283:41-8. 2002
    ..Consistent with our previous reports, most of the new members of the three subunits show a unique tissue-specific pattern, while another AtNF-YC9 is rather ubiquitous...
  33. ncbi Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana
    G Gusmaroli
    Dipartimento di Genetica e Biologia dei Microrganismi, , Via Celoria 26, 20133 Milan, Italy
    Gene 264:173-85. 2001
    ....
  34. ncbi Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation
    Silvia Di Agostino
    Experimental Oncology Department, Istituto Regina Elena, Via delle Messi d Oro 156, 00158 Rome, Italy
    Cancer Cell 10:191-202. 2006
    ..Endogenous NF-Y, mutant p53, and p300 proteins form a triple complex upon DNA damage. We demonstrate that aberrant transcriptional regulation underlies the ability of mutant p53 proteins to act as oncogenic factors...
  35. ncbi Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer
    S L Forsburg
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Genes Dev 3:1166-78. 1989
    ..Replacement of this region with the activation domain of GAL4 restored activity, suggesting that it provides the principal activation domain to the bound HAP2/3/4 complex...
  36. pmc NF-Ya activates multiple hematopoietic stem cell (HSC) regulatory genes and promotes HSC self-renewal
    Jiang Zhu
    Department of Medicine, and Abramson Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:11728-33. 2005
    ..Thus, NF-Ya is a potent cellular regulator of HSC self-renewal...
  37. pmc Role of NF-Y in in vivo regulation of the gamma-globin gene
    Z Duan
    Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Biol 21:3083-95. 2001
    ..These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR...
  38. ncbi Modulating the distribution of fluxes among respiration and fermentation by overexpression of HAP4 in Saccharomyces cerevisiae
    A J van Maris
    Kluwer Laboratory of Biotechnology, Delft University of Technology, The Netherlands
    FEMS Yeast Res 1:139-49. 2001
    ..The effect of Hap4p overproduction was most drastic in aerobic, glucose-grown chemostat cultures in which ammonium was limiting. In such cultures, the biomass yield on glucose was double that of the wild-type...
  39. ncbi Identification, characterization and interaction of HAP family genes in rice
    Thiruvengadam Thirumurugan
    Genetic Strains Research Center, National Institute of Genetics, Shizuoka ken, Japan
    Mol Genet Genomics 279:279-89. 2008
    ..This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members...
  40. pmc Characterization of the human topoisomerase IIbeta (TOP2B) promoter activity: essential roles of the nuclear factor-Y (NF-Y)- and specificity protein-1 (Sp1)-binding sites
    Chun Nam Lok
    Cancer Research Laboratories and Department of Pharmacology and Toxicology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Biochem J 368:741-51. 2002
    ..Our results suggest that the binding sites for NF-Y and Sp1 are critical for TOP2B transcription...
  41. ncbi Oct-1 and nuclear factor Y bind to the SURG-1 element to direct basal and gonadotropin-releasing hormone (GnRH)-stimulated mouse GnRH receptor gene transcription
    Kyung Yoon Kam
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Endocrinol 19:148-62. 2005
    ..In conclusion, NF-Y and Oct-1 bind to the SURG-1 element to direct basal and GnRH-stimulated expression of the mGnRHR gene...
  42. ncbi CCAAT/enhancer-binding protein alpha (C/EBPalpha) activates transcription of the human microsomal epoxide hydrolase gene (EPHX1) through the interaction with DNA-bound NF-Y
    Qin Shi Zhu
    Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 279:29902-10. 2004
    ..C/EBPalpha-dependent EPHX1 activation was also supported by reconstitution studies in HeLa cells that lack this protein. These results establish that EPHX1 expression is regulated by C/EBPalpha interacting with DNA-bound NF-Y...
  43. ncbi Nuclear factor Y (NF-Y) and cellular senescence
    K Matuoka
    Graduate Program in Molecular Biosciences, and, Rutgers The State University of New Jersey, Piscataway, New Jersey 08854 8087, USA
    Exp Cell Res 253:365-71. 1999
    ..The regulation of thymidine kinase (TK) and dihydrofolate reductase (DHFR) genes in human diploid fibroblasts serves as an example of how NF-Y may have a role in replicative senescence by regulating age-dependent G1/S genes...
  44. pmc Developmentally specific role of the CCAAT box in regulation of human gamma-globin gene expression
    Xiangdong Fang
    Division of Medical Genetics, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 279:5444-9. 2004
    ..Our data also suggest that it is unlikely that transcriptional stimulation by the CCAAT box is exerted through direct protein-protein interaction between NF-Y and TBP...
  45. pmc The Schizosaccharomyces pombe homolog of Saccharomyces cerevisiae HAP2 reveals selective and stringent conservation of the small essential core protein domain
    J T Olesen
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Mol Cell Biol 11:611-9. 1991
    ..cerevisiae, the S. pombe gene is specifically involved in mitochondrial function. This contrasts to the situation in mammals, in which the homologous CCAAT-binding complex is a global transcriptional activator...
  46. ncbi Involvement of NF-Y and Sp1 binding sequences in basal transcription of the human telomerase RNA gene
    Jiangqin Zhao
    Cancer Research UK Department of Medical Oncology, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    FEBS Lett 536:111-9. 2003
    ..Thus the interaction of NF-Y at the CCAAT box is pivotal to hTR gene transcription and surrounding sequence elements may provide an environment for the regulation of activity through recruitment of additional protein complexes...
  47. pmc Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells
    Marica Grskovic
    Institute for Regeneration Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS Genet 3:e145. 2007
    ..Our results suggest that this systematic approach can be broadly applied to understanding transcriptional networks in mammalian species...
  48. ncbi Expression of the cell cycle phosphatase cdc25C is down-regulated by the tumor suppressor protein p53 but not by p73
    K Krause
    Department of Internal Medicine II, University of Leipzig, , Johannisallee 30, D-04103 Leipzig, Germany
    Biochem Biophys Res Commun 284:743-50. 2001
    ..This reveals functional differences between p73 and p53 in regulating cell division...
  49. pmc Hap4p overexpression in glucose-grown Saccharomyces cerevisiae induces cells to enter a novel metabolic state
    Romeo Lascaris
    Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands
    Genome Biol 4:R3. 2003
    ..To understand why overexpression of HAP4 is able to override the signals that normally result in glucose repression of mitochondrial function, we analyzed in detail the changes that occur in these cells...
  50. pmc Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis
    D Edwards
    Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, United Kingdom
    Plant Physiol 117:1015-22. 1998
    ..The unexpected presence of multiple forms of each HAP homolog in Arabidopsis, compared with the single genes in yeast and vertebrates, suggests that the HAP2,3,5 complex may play diverse roles in gene transcription in higher plants...
  51. ncbi NF-Y cooperates with USF1/2 to induce the hematopoietic expression of HOXB4
    Jiang Zhu
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Blood 102:2420-7. 2003
    ..These results suggest that NF-Y is a developmentally regulated inducer of the HOXB4 gene in hematopoietic cells...
  52. ncbi Transcription factors NF-YA regulate the induction of human OGG1 following DNA-alkylating agent methylmethane sulfonate (MMS) treatment
    Mi Rha Lee
    Research Center for Proteineous Materials, Chosun University, 375 Seosuk dong, Gwangju 501 759, Korea
    J Biol Chem 279:9857-66. 2004
    ....
  53. ncbi The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation
    Anuradha Bhattacharya
    Department of Molecular Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 63:8167-72. 2003
    To understand the physiological function of the mammalian heterotrimeric CCAAT binding factor CBF, also known as NF-Y, we have generated a conditional Cbf-b mouse mutant by introducing loxP sites in the murine Cbf-b/Nf-ya gene...
  54. ncbi Three CCAAT-boxes and a single cell cycle genes homology region (CHR) are the major regulating sites for transcription from the human cyclin B2 promoter
    Mark Wasner
    Medizinische Klinik und Poliklinik II, Max Bürger Forschungszentrum, Universitat Leipzig, Johannisallee 30, D 04103 Leipzig, Germany
    Gene 312:225-37. 2003
    ..Together with the recently described mouse cdc25C promoter, human cyclin B2 is the second identified gene which solely requires a CHR for its cell cycle regulation...
  55. pmc CCAAT-binding factor regulates expression of the beta1 subunit of soluble guanylyl cyclase gene in the BE2 human neuroblastoma cell line
    Iraida G Sharina
    Department of Integrative Biology and Pharmacology and Institute of Molecular Medicine, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:11523-8. 2003
    ..In summary, our study represents an analysis of the human beta1 sGC promoter regulation in human neuroblastoma BE2 cells and identifies CBF as a critically important factor in beta1 sGC expression...
  56. ncbi A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants
    M Kato
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa ku, 464 8601, Nagoya, Japan
    FEBS Lett 512:227-9. 2002
    ..From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex...
  57. ncbi Trichostatin A induces transforming growth factor beta type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1.NF-Y complex
    Weiqi Huang
    Department of Medicine, Division of Medical Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, Texas 78229 3900, USA
    J Biol Chem 280:10047-54. 2005
    ..NF-Y.p300.PCAF.HDAC-1 multiprotein complex. Moreover, the interaction of NF-Y with the Sp1-associated complex may further explain why this specific Sp1 site mediates transcriptional responsiveness to TSA...
  58. ncbi Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells
    Hirohiko Okamura
    Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 18 15, 3 Kuramoto cho, Tokushima 770 8504, Japan
    Int J Oncol 25:1031-7. 2004
    ..NF-YA protein was expressed at higher levels in SCCTF cells than that in SCCKN cells. Hoechst dye staining also showed that MDR1 protein acts more effectively as an efflux pump in SCCTF cells than that in SCCKN cells...
  59. ncbi Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters
    Giuseppina Caretti
    Dipartimento di Biologia Animale, Università di Modena e Reggio, Via Campi 213 D, 41100 Modena, Italy
    J Biol Chem 278:30435-40. 2003
    ..These data indicate that following the release of E2Fs/HDACs, a hierarchy of PCAF-NF-Y-p300 interactions and H3-H4 acetylations are required for activation of cell-cycle promoters...
  60. pmc Regulation of human methylthioadenosine phosphorylase gene by the CBF (CCAAT binding factor)/NF-Y (nuclear factor-Y)
    Yuwaraj Kadariya
    Department of Laboratory Medicine, Mie University School of Medicine, Tsu, Mie 514 8507, Japan
    Biochem J 387:175-83. 2005
    ..Our results show that the hMTAP expression is regulated by a CBF and that the distal one of two CCAAT motifs plays a major role in the transcriptional activation of hMTAP gene...
  61. pmc Modulation of DNA topoisomerase II alpha promoter activity by members of the Sp (specificity protein) and NF-Y (nuclear factor Y) families of transcription factors
    Natisha Magan
    Institute of Molecular Biosciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand
    Biochem J 374:723-9. 2003
    ..Sp1 and NF-Y were both found to be transcriptional modulators with activator or repressor functions depending on protein/DNA context. Moreover, a functional interaction between Sp1 and NF-Y bound at proximal elements was observed...
  62. ncbi Cdk2-dependent phosphorylation of the NF-Y transcription factor is essential for the expression of the cell cycle-regulatory genes and cell cycle G1/S and G2/M transitions
    Hee Don Chae
    National Research Laboratory, Department of Microbiology, Dankook University College of Medicine, Cheonan 330 714, Korea
    Oncogene 23:4084-8. 2004
    ..These results suggest that cdk2-dependent phosphorylation of NF-Y is essential for the expression of the cell cycle-regulatory genes and therefore for cell cycle progression at both G1/S and G2/M...
  63. ncbi p73 competes with co-activators and recruits histone deacetylase to NF-Y in the repression of PDGF beta-receptor
    Hidetaka Uramoto
    Department of Cell Biology, Institute of Anatomy and Cell Biology, Goteborg University, Box 420, SE 405 30 Gothenburg, Sweden
    J Cell Sci 117:5323-31. 2004
    ..These results suggest that p73 and DeltaNp73 behave as physiological regulators for the transcription of the PDGFRB promoter...
  64. pmc Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro
    Chandrani Chattopadhyay
    Department of Molecular Genetics, M D Anderson Cancer Center and Genes, Graduate School of Biomedical Sciences, The University of Texas, Houston, TX 77030, USA
    Nucleic Acids Res 32:3632-41. 2004
    ..Altogether, our study identified p32 as a novel and specific corepressor of CBF-mediated transcription activation in vitro...
  65. pmc GATA-1 and NF-Y cooperate to mediate erythroid-specific transcription of Gfi-1B gene
    Duen Yi Huang
    Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, No 1 Jen Ai Road 1st section, Taipei, Taiwan, Republic of China
    Nucleic Acids Res 32:3935-46. 2004
    ..Here we conclude that functional cooperation between GATA-1 and NF-Y contributes to erythroid-specific transcriptional activation of Gfi-1B promoter...
  66. ncbi p73 independent of c-Myc represses transcription of platelet-derived growth factor beta-receptor through interaction with NF-Y
    Anders Hackzell
    Department of Cell Biology, Institute of Anatomy and Cell Biology, Goteborg University, Box 420, SE 405 30 Gothenburg, Sweden
    J Biol Chem 277:39769-76. 2002
    ..Our results indicate that serum stimulation induces c-Myc and p73alpha, leading to the down-regulation of PDGF beta-receptor expression by repressing its transcription...
  67. pmc NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes
    Yasuaki Kabe
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Mol Cell Biol 25:512-22. 2005
    ....
  68. ncbi Expression of the Op18 gene is maintained by the CCAAT-binding transcription factor NF-Y
    Houda Benlhabib
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, 55455, USA
    Gene 377:177-85. 2006
    ..These data suggest that NF-Y is a major transcription factor promoting expression of Op18...
  69. ncbi Hap4 is not essential for activation of respiration at low specific growth rates in Saccharomyces cerevisiae
    Vijayendran Raghevendran
    Center for Microbial Biotechnology, Building 223, BioCentrum DTU, Technical University of Denmark, DK 2800, Kongens Lyngby, Denmark
    J Biol Chem 281:12308-14. 2006
    ....
  70. ncbi The Aspergillus nidulans multimeric CCAAT binding complex AnCF is negatively autoregulated via its hapB subunit gene
    S Steidl
    Institut fur Mikrobiologie und Genetik, Technische Universitat Darmstadt, Schnittspahnstrasse 10, Darmstadt, D 64287, Germany
    J Mol Biol 306:643-53. 2001
    ..In the fungus Aspergillus nidulans the respective complex was designated AnCF (A. nidulans CCAAT binding factor). AnCF is composed of at least three subunits designated HapB, HapC and HapE...
  71. pmc Transcriptional regulators of the Schizosaccharomyces pombe fbp1 gene include two redundant Tup1p-like corepressors and the CCAAT binding factor activation complex
    R T Janoo
    Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, USA
    Genetics 157:1205-15. 2001
    ..Deletion of php5 reduces fbp1 expression under both repressed and derepressed conditions. The CBF appears to act in parallel to atf1-pcr1, although it is unclear whether or not CBF activity is regulated by PKA...
  72. ncbi Role of an inverted CCAAT element in human topoisomerase IIalpha gene expression in ICRF-187-sensitive and -resistant CEM leukemic cells
    S E Morgan
    Division of Molecular Pharmacology, Departments of Molecular Genetics, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    Mol Pharmacol 59:203-11. 2001
    ..This is the first study to show that topoIIalpha transcriptional up-regulation in ICRF-187-resistant cells is mediated in part by altered regulation of the third inverted CCAAT box in the topoIIalpha promoter...
  73. ncbi Functional interaction of NF-Y and Sp1 is required for type a natriuretic peptide receptor gene transcription
    F Liang
    Metabolic Research Unit and Department of Medicine, University of California, San Francisco, California 94143 0540, USA
    J Biol Chem 276:1516-22. 2001
    ..Together, these studies show that NPR-A promoter activity is dominantly regulated through functional, and possibly physical, interactions of NF-Y and Sp1...
  74. ncbi NF-Y and CCAAT/enhancer-binding protein alpha synergistically activate the mouse amelogenin gene
    Yucheng Xu
    Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 281:16090-8. 2006
    ..These results suggest that C/EBPalpha and NF-Y synergistically activate the mouse amelogenin gene and can contribute to its physiological regulation during amelogenesis...
  75. pmc Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo
    Q Li
    Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
    EMBO J 17:6300-15. 1998
    ..We suggest two steps in chromatin modification at the Xenopus hsp70 promoter: first the binding of NF-Y to the Y-boxes to pre-set chromatin and second the recruitment of p300 to modulate transcriptional activity...
  76. pmc Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters
    Giacomo Donati
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    Nucleic Acids Res 34:3116-27. 2006
    ..These data highlight a previously unappreciated complexity of TFs binding and epigenetic changes, pointing to different TFs-specific pathways within this broad response...
  77. ncbi Repression of new p53 targets revealed by ChIP on chip experiments
    Michele Ceribelli
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    Cell Cycle 5:1102-10. 2006
    ..Finally, there is a large overlap (66%) between p53 and NF-Y targets. Our data reinstate the physiological importance of p53 promoter recognition and direct transcriptional repression as a mechanism to cope with DNA-damage...
  78. pmc Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae
    David S McNabb
    Department of Biological Sciences, SCEN601, University of Arkansas, Fayetteville, AR 72701, USA
    Eukaryot Cell 4:1829-39. 2005
    ....
  79. ncbi The Aspergillus nidulans homoaconitase gene lysF is negatively regulated by the multimeric CCAAT-binding complex AnCF and positively regulated by GATA sites
    G Weidner
    Institut fur Mikrobiologie und Genetik, Technische Universitat Darmstadt, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Arch Microbiol 175:122-32. 2001
    ..Results of Northern blot analysis also excluded that the global nitrogen regulator AreA is the responsible trans-acting GATA-binding factor...
  80. ncbi NF-Y recruitment of TFIID, multiple interactions with histone fold TAF(II)s
    Mattia Frontini
    Dipartimento di Biologia Animale, Università di Modena e Reggio, Via Campi 213 D, Modena 41100, Italy
    J Biol Chem 277:5841-8. 2002
    ..These results indicate that NF-Y (i) recruits purified holo-TFIID in vitro and (ii) can associate multiple TAF(II)s, potentially accommodating different core promoter architectures...
  81. ncbi Tuberin regulates the DNA repair enzyme OGG1
    Samy L Habib
    George O Brien Kidney Research Center, Department of Medicine, University of Texas Health Science Center, San Antonio, TX 78229, USA
    Am J Physiol Renal Physiol 294:F281-90. 2008
    ..These data provide the first evidence that tuberin regulates a specific DNA repair enzyme, OGG1. This regulation may be important in the pathogenesis of kidney tumors in patients with TSC...
  82. ncbi CBF/NF-Y functions both in nucleosomal disruption and transcription activation of the chromatin-assembled topoisomerase IIalpha promoter. Transcription activation by CBF/NF-Y in chromatin is dependent on the promoter structure
    F Coustry
    Department of Molecular Genetics, The University of Texas, M. D. Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 276:40621-30. 2001
    ..It also suggests that specific promoter structure may play a role in the CBF-mediated transcription activation of nucleosomal topo IIalpha promoter template...
  83. pmc The B subunit of a rat heteromeric CCAAT-binding transcription factor shows a striking sequence identity with the yeast Hap2 transcription factor
    S N Maity
    Department of Molecular Genetics, University of Texas, M D Anderson Cancer Center, Houston 77030
    Proc Natl Acad Sci U S A 87:5378-82. 1990
    ..Hence, only a few changes in one domain of this protein were tolerated during evolution between yeast and mammals, whereas the rest of the protein diverged much more extensively...
  84. ncbi Transcription factor NFY globally represses the expression of the C. elegans Hox gene Abdominal-B homolog egl-5
    Hansong Deng
    Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, PR China
    Dev Biol 308:583-92. 2007
    ..In addition, we show that the NFY complex interacts with the MES-2/MES-6 PcG complex in Hox gene regulation. Thus, our studies unravel a physiological function of NFY in establishing the spatially restricted expression pattern of egl-5...
  85. ncbi Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding
    D S McNabb
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Genes Dev 9:47-58. 1995
    ..These data suggest that the HAP2/3/5 heterotrimer represents a unique DNA-binding factor in which all three subunits of the complex are absolutely required for DNA-binding activity...
  86. pmc Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3
    S Sinha
    Department of Molecular Genetics, University of Texas, M D Anderson Cancer Center, Houston 77030
    Proc Natl Acad Sci U S A 92:1624-8. 1995
    The CCAAT binding factor CBF is a heteromeric transcription factor, which binds to functional CCAAT motifs in many eukaryotic promoters...
  87. ncbi Comparative integromics on FZD7 orthologs: conserved binding sites for PU.1, SP1, CCAAT-box and TCF/LEF/SOX transcription factors within 5'-promoter region of mammalian FZD7 orthologs
    Masuko Katoh
    M and M Medical BioInformatics, Hongo 113 0033, Japan
    Int J Mol Med 19:529-33. 2007
    ..Comparative genomics analyses revealed that the binding sites for PU.1, SP1/Krüppel-like, CCAAT-box, and TCF/LEF/SOX transcription factors were conserved among 5'-promoter regions of mammalian FZD7 orthologs...
  88. ncbi Temperature may influence and regulate NF-YB expression in toad oocyte
    Shan Yan
    Shanghai Medical College, Fudan University, Shanghai 200032, PR China
    Biochem Biophys Res Commun 313:802-11. 2004
    ..Taken together, this report of the differential expression of toad NF-YB at different temperatures is the first evidence that temperature may influence and regulate NF-YB expression...
  89. ncbi Isolation and characterization of the rat SND p102 gene promoter: putative role for nuclear factor-Y in regulation of transcription
    Lorena Rodriguez
    Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Sarriena s n, 48940 Leioa, Bizkaia, Spain
    Ann N Y Acad Sci 1091:282-95. 2006
    ..The absence of TATA box and the location and reverse orientation of the CCAAT boxes in the promoter region strongly suggest a role for NF-Y in the regulation of transcription of SND p102 gene...
  90. ncbi Molecular cloning and characterization of alpha1-soluble guanylyl cyclase gene promoter in rat pituitary cells
    Yonghua Jiang
    Section on Cellular Signaling, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 4510, USA
    J Mol Endocrinol 37:503-15. 2006
    ..These results indicate that the composite CCAAT/Sp1 cis-element contributes to the expression of alpha1-sGC subunit in resting pituitary cells...
  91. ncbi Targeting the inverted CCAAT Box-2 of the topoisomerase IIalpha gene: DNA sequence selective recognition by a polyamide-intercalator as a staggered dimer
    Hilary Mackay
    Department of Chemistry, Furman University, Greenville, SC 29613, USA
    Bioorg Med Chem 16:2093-102. 2008
    ..These results suggest that the conjugate can enter the nucleus, bind to its target site, presumably as a stacked dimer, and up-regulate the expression of topoIIalpha by blocking the binding of NF-Y...
  92. pmc Polyunsaturated fatty acid suppression of fatty acid synthase (FASN): evidence for dietary modulation of NF-Y binding to the Fasn promoter by SREBP-1c
    Margarita Teran-Garcia
    Department of Adipocyte Signalling, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA
    Biochem J 402:591-600. 2007
    ....
  93. ncbi Transcriptional regulation of the human reduced folate carrier promoter C: synergistic transactivation by Sp1 and C/EBP beta and identification of a downstream repressor
    Scott G Payton
    Department of Pharmacology, Wayne State University, School of Medicine, Detroit, MI, USA
    Biochim Biophys Acta 1727:45-57. 2005
    ..Another possible factor in the tissue-specific regulation of the hRFC-C region involves the downstream repressor flanking the minimal promoter...
  94. ncbi Down-regulation of human topoisomerase IIalpha correlates with altered expression of transcriptional regulators NF-YA and Sp1
    Kirsty A Allen
    Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
    Anticancer Drugs 15:357-62. 2004
    ..Of these, three showed concomitant changes in the expression of Sp1 and NF-YA. Thus, we provide the first evidence for roles of Sp1 and NF-Y in bringing about the drug-induced down-regulation of Topo IIalpha gene expression...
  95. ncbi SOX7 and SOX17 regulate the parietal endoderm-specific enhancer activity of mouse laminin alpha1 gene
    Tomoaki Niimi
    Sekiguchi Biomatrix Signaling Project, ERATO, Japan Science and Technology Agency, Aichi Medical University, Karimata, Yazako, Nagakute, Aichi 480 1195, Japan
    J Biol Chem 279:38055-61. 2004
    ....
  96. ncbi Involvement of specific proteins (Sp1/Sp3) and nuclear factor Y in basal transcription of the distal promoter of the rat pyruvate carboxylase gene in beta-cells
    Piyanate Sunyakumthorn
    Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    Biochem Biophys Res Commun 329:188-96. 2005
    ..Electrophoretic mobility shift assays (EMSAs) and supershift EMSAs using nuclear extract from INS-1 cells demonstrated that Sp1 and Sp3 bind a GC box while the nuclear factor Y was shown to bind the proximal but not the distal CCAAT box...
  97. ncbi The gene encoding acyl-CoA-binding protein is subject to metabolic regulation by both sterol regulatory element-binding protein and peroxisome proliferator-activated receptor alpha in hepatocytes
    Maria B Sandberg
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark
    J Biol Chem 280:5258-66. 2005
    ..In conclusion, expression of ACBP in rodent hepatocytes is subject to dual metabolic regulation by PPARalpha and SREBP-1c, which may reflect the need for ACBP during lipogenic as well as lipo-oxidative conditions...
  98. ncbi Characterization of the 5'-flanking region of the human transcription factor Sp3 gene
    Alicia Tapias
    Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Avenue Diagonal 643, Barcelona E 08028, Spain
    Biochim Biophys Acta 1730:126-36. 2005
    ..Furthermore, binding of the NF-Y complex to the CCAAT box located in the proximal promoter of Sp3 was observed in gel-shift assays...
  99. pmc The expression of the human neuronal alpha3 Na+,K+-ATPase subunit gene is regulated by the activity of the Sp1 and NF-Y transcription factors
    Roberta Benfante
    Department of Pharmacology, School of Medicine, University of Milan, 32 via Vanvitelli, 20129 Milan, Italy
    Biochem J 386:63-72. 2005
    ..Although the expression of these factors is not tissue-specific, co-operative functional interactions among them are required to direct the activity of the promoter predominantly in neuronal cells...
  100. ncbi Transcriptional regulation of the mouse PNRC2 promoter by the nuclear factor Y (NFY) and E2F1
    Dujin Zhou
    Department of Surgical Research, Beckman Research Institute of City of Hope, Duarte, California 91010, USA
    Gene 361:89-100. 2005
    ....
  101. ncbi Cell-specific regulation of TRBP1 promoter by NF-Y transcription factor in lymphocytes and astrocytes
    Sylvie Bannwarth
    Molecular Oncology Group Lady Davis Institute for Medical Research, Montreal, QC, Canada, H3T 1E2
    J Mol Biol 355:898-910. 2006
    ..These studies suggest that the cell specifity of HIV-1 expression and replication may be regulated, in part, through the control of TRBP1 expression...

Research Grants15

  1. Role of EZH2 in Epithelial Ovarian Cancer
    Rugang Zhang; Fiscal Year: 2013
    ..In addition, the mechanistic insights gained from the current studies have broad implications for many different types of cancers as well. ..
  2. TRANSCRIPTIONAL REGULATION OF TYPE I COLLAGEN GENES BY C
    Sankar Maity; Fiscal Year: 1999
    ..One critical transacting factor is a CCAAT binding factor (CBF) that activates in vitro transcript ion of both the alpha(l) and alpha(l) collagen promoters as well ..
  3. ROLE OF CBF/NF-Y IN TRANSCRIPTION: TYPE I COLLAGEN GENES
    Sankar Maity; Fiscal Year: 2004
    ....
  4. CELLULAR MECHANISMS IN SYNTHESIS OF EXTRACELLULAR MATRIX
    Benoit de Crombrugghe; Fiscal Year: 2003
    The CCAAT Binding Factor CBF, also known as NFY, is a ubiquitous transcription factor which binds to the proximal promoters of the two type I collagen genes and to cognate sites in many other eucaryotic promoters, including promoters that ..
  5. TETRAHYDROBIOPTERIN BIOSYNTHESIS BY DOPAMINE NEURONS
    Gregory Kapatos; Fiscal Year: 2008
    ..We expect that this multidisciplinary approach will yield important new information on the role of GCH1 in NSDA neuron function and will lead to a new understanding of DRD and familial and idiopathic PD. ..
  6. REGULATION OF HIV2 TRANSCRIPTION AND REPLICATION
    DAVID MARKOVITZ; Fiscal Year: 2002
    ..These studies should cast light on how cellular proteins contribute to the regulation of HIV-2 and HIV-1 transcription and replication and suggest novel therapeutic interventions for both viruses. ..
  7. CXC Chemokines and HIV Pathogenesis
    DAVID MARKOVITZ; Fiscal Year: 2009
    ..A mechanistic understanding of the interactions between HIV and these C-X-C chemokines could lead to the development of new approaches to the treatment of patients infected with HIV. ..
  8. Sequence Determinants of Meiotic Recombination Hotspots
    Walter Steiner; Fiscal Year: 2007
    ..pombe are also active in the distantly related budding yeast Saccharomyces cerevisiae. Sequences that are active in both organisms may be widely conserved among eukaryotes, including man. [unreadable] [unreadable] [unreadable]..
  9. C-X-C Chemokines and Kaposi's Sarcoma
    DAVID MARKOVITZ; Fiscal Year: 2005
    ..This work thus has the potential to link KSHV, growth factors, and HIV/Tat in the pathogenesis of KS, and to suggest new avenues for the treatment of this complex neoplasm. ..
  10. MAP KINASE REGULATION OF MICROGLIAL ACTIVATION
    Narayan Bhat; Fiscal Year: 2004
    ..The suppressive effects of the kinase inhibitors on activation-associated microglial functions; i.e., targeting of oligodendrocytes and myelin phagocytosis will be tested using in vitro models. ..
  11. The role of the CCAAT-binding factor in Candida albicans
    DAVID MCNABB; Fiscal Year: 2007
    ..albicans virulence; and 3) to dissect the regulatory function of the CCAAT-binding factor. ..
  12. GROWTH FACTORS IN FOLLICULAR DEVELOPMENT
    Shyamal Roy; Fiscal Year: 2001
    ..The information will shed light on the mechanisms involved in hormonal control of folliculogenesis via intraovarian growth factors. ..
  13. Neuroinflammation in Cholesterol-Induced AD Pathogenesis
    Narayan Bhat; Fiscal Year: 2009
    ..The outcome of these studies should have implications for developing novel and effective anti- inflammatory treatments for AD. ..
  14. MAP KINASES IN OLIGODENDROCYTE CELL SIGNALING
    Narayan Bhat; Fiscal Year: 2005
    ..Putative MAPK-responsive elements and relevant binding activities will be determined by deletion and gel shift analyses ..
  15. NEGATIVE REGULATION OF GENE EXPRESSION BY VITAMIN D
    NICHOLAS KOSZEWSKI; Fiscal Year: 2002
    ..abstract_text> ..