ccaat enhancer binding protein alpha

Summary

Summary: A CCAAT-enhancer-binding protein found in LIVER; ADIPOSE TISSUE; INTESTINES; LUNG; ADRENAL GLANDS; PLACENTA; OVARY and peripheral blood mononuclear cells (LEUKOCYTES, MONONUCLEAR). Experiments with knock-out mice have demonstrated that CCAAT-enhancer binding protein-alpha is essential for the functioning and differentiation of HEPATOCYTES and ADIPOCYTES.

Top Publications

  1. ncbi Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha
    Pu Zhang
    Harvard Institutes of Medicine, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Immunity 21:853-63. 2004
  2. pmc c-Myc is a critical target for c/EBPalpha in granulopoiesis
    L M Johansen
    Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 21:3789-806. 2001
  3. ncbi E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo
    B T Porse
    Laboratory of Gene Therapy Research, 2100 Copenhagen Ø, Denmark
    Cell 107:247-58. 2001
  4. pmc C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway
    Evan D Rosen
    Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genes Dev 16:22-6. 2002
  5. ncbi Down-regulation and antiproliferative role of C/EBPalpha in lung cancer
    Balazs Halmos
    Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cancer Res 62:528-34. 2002
  6. ncbi C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4
    H Wang
    Department of Pathology and Huffington Center on Aging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell 8:817-28. 2001
  7. pmc Dysregulation of the C/EBPalpha differentiation pathway in human cancer
    Steffen Koschmieder
    University of Munster, Munster, Germany
    J Clin Oncol 27:619-28. 2009
  8. ncbi Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta during adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated repression of HDAC1 at the C/ebp alpha gene promoter
    Ying Zuo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 281:7960-7. 2006
  9. ncbi Transcription activation function of C/EBPalpha is required for induction of granulocytic differentiation
    Karen Keeshan
    Thomas Jefferson Medical College, Kimmel Cancer Institute, Philadelphia, PA 19107, USA
    Blood 102:1267-75. 2003
  10. pmc Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis
    Yojiro Arinobu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:18105-10. 2005

Research Grants

  1. Molecular mechanisms of C/EBP alpha mediated growth arrest
    Nikolai A Timchenko; Fiscal Year: 2013
  2. SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION
    Richard N Day; Fiscal Year: 2012
  3. Regulation of adipocyte differentiation and metabolism
    Ormond A MacDougald; Fiscal Year: 2010
  4. The Role of C/EBPa in Genome Stability and Tumor Progression
    JONATHAN RUSSELL HALL; Fiscal Year: 2012
  5. Role of C/EBPalpha in Cytoprotection and Recovery from Lung Injury
    Machiko Ikegami; Fiscal Year: 2012
  6. Role of Age in Liver Cancer
    Nikolai A Timchenko; Fiscal Year: 2013
  7. Targeting the C/EBPalpha-Gfi-pathway in CML stem cells
    Bruno Calabretta; Fiscal Year: 2013
  8. Regulation of CYP2B6 in Human Liver
    Hongbing Wang; Fiscal Year: 2013
  9. METABOLIC HOMEOSTASIS IN LIVER REGENERATION
    LINDA GREENBAUM; Fiscal Year: 1999
  10. Transcription & Function of Adipocyte Beta3AR in Obesity
    Sheila Collins; Fiscal Year: 2006

Detail Information

Publications205 found, 100 shown here

  1. ncbi Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha
    Pu Zhang
    Harvard Institutes of Medicine, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Immunity 21:853-63. 2004
    ..Therefore, C/EBP alpha is not only essential for granulocyte development but, in addition, is a regulator of hematopoietic stem cell activity...
  2. pmc c-Myc is a critical target for c/EBPalpha in granulopoiesis
    L M Johansen
    Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 21:3789-806. 2001
    ..This is the first report to demonstrate that C/EBPalpha directly affects the level of c-Myc expression and, thus, the decision of myeloid blasts to enter into the granulocytic differentiation pathway...
  3. ncbi E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo
    B T Porse
    Laboratory of Gene Therapy Research, 2100 Copenhagen Ø, Denmark
    Cell 107:247-58. 2001
    ....
  4. pmc C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway
    Evan D Rosen
    Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genes Dev 16:22-6. 2002
    ..These results indicate that C/EBPalpha and PPARgamma participate in a single pathway of fat cell development with PPARgamma being the proximal effector of adipogenesis...
  5. ncbi Down-regulation and antiproliferative role of C/EBPalpha in lung cancer
    Balazs Halmos
    Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cancer Res 62:528-34. 2002
    ..Genetic analysis of the C/EBPalpha gene is in progress to fully evaluate its role as a novel tumor suppressor in lung cancer...
  6. ncbi C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4
    H Wang
    Department of Pathology and Huffington Center on Aging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell 8:817-28. 2001
    ..Our data demonstrate that the liver-specific transcription factor C/EBPalpha brings about growth arrest through direct inhibition of cdk2 and cdk4...
  7. pmc Dysregulation of the C/EBPalpha differentiation pathway in human cancer
    Steffen Koschmieder
    University of Munster, Munster, Germany
    J Clin Oncol 27:619-28. 2009
    ..The differentiation-inducing transcription factor CCAAT enhancer binding protein alpha (C/EBPalpha) is required for proper control of adipogenesis, glucose metabolism, granulocytic ..
  8. ncbi Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta during adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated repression of HDAC1 at the C/ebp alpha gene promoter
    Ying Zuo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 281:7960-7. 2006
    ....
  9. ncbi Transcription activation function of C/EBPalpha is required for induction of granulocytic differentiation
    Karen Keeshan
    Thomas Jefferson Medical College, Kimmel Cancer Institute, Philadelphia, PA 19107, USA
    Blood 102:1267-75. 2003
    ....
  10. pmc Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis
    Yojiro Arinobu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:18105-10. 2005
    ..Thus, circulating basophils and tissue mast cells share a common developmental stage at which their fate decision might be controlled principally by C/EBPalpha...
  11. ncbi Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes
    Deepanwita Prusty
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:46226-32. 2002
    ..Our results suggest that activation of the MEK/ERK signaling pathway during the initial 12 h of adipogenesis enhances the activity of factors that regulate both C/EBPalpha and PPARgamma expression...
  12. ncbi A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBPalpha regulates human granulopoiesis
    Francesco Fazi
    Department of Histology and Medical Embryology, University of Rome La Sapienza and San Raffaele Biomedical Science Park of Rome, Via di Castel Romano 100, 00128, Rome, Italy
    Cell 123:819-31. 2005
    ..Altogether, our data indicate that miR-223 plays a crucial role during granulopoiesis and point to the NFI-A repression as an important molecular pathway mediating gene reprogramming in this cell lineage...
  13. pmc Genetic ablation of CCAAT/enhancer binding protein alpha in epidermis reveals its role in suppression of epithelial tumorigenesis
    Kari D Loomis
    Cell Signaling and Cancer Group, Department of Environmental and Molecular Toxicology and Functional Genomics Program, North Carolina State University, Raleigh, NC 27695, USA
    Cancer Res 67:6768-76. 2007
    ..Our study shows that C/EBPalpha is dispensable for epidermal homeostasis and provides genetic evidence that C/EBPalpha is a suppressor of epithelial tumorigenesis...
  14. pmc Absence of the transcription factor CCAAT enhancer binding protein alpha results in loss of myeloid identity in bcr/abl-induced malignancy
    Katharina Wagner
    Harvard Institutes of Medicine, Room 954, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:6338-43. 2006
    The lineage-determining transcription factor CCAAT enhancer binding protein alpha (C/EBPalpha) is required for myeloid differentiation. Decreased function or expression of C/EBPalpha is often found in human acute myeloid leukemia...
  15. pmc Phosphorylation of C/EBPalpha inhibits granulopoiesis
    Sarah E Ross
    Department of Molecular and Integrative Physiology, University of Michigan Medical School, 1301 E Catherine Street, Ann Arbor, MI 48109 0622, USA
    Mol Cell Biol 24:675-86. 2004
    ..Thus, myeloid development is partly regulated by an ERK1/2-mediated change in the conformation of C/EBPalpha that favors monocyte differentiation by blocking granulopoiesis...
  16. ncbi Lithium stabilizes the CCAAT/enhancer-binding protein alpha (C/EBPalpha) through a glycogen synthase kinase 3 (GSK3)-independent pathway involving direct inhibition of proteasomal activity
    Minsub Shim
    Cell Signaling and Cancer Group, Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh 27695 7633, USA
    J Biol Chem 278:19674-81. 2003
    ..These results demonstrate C/EBPalpha is degraded via a ubiquitin-dependent proteasomal pathway, and LiCl stabilizes C/EBPalpha through a GSK3-independent pathway involving direct inhibition of proteasome activity...
  17. ncbi Dysfunctional interaction of C/EBPalpha and the glucocorticoid receptor in asthmatic bronchial smooth-muscle cells
    Michael Roth
    Department of Pharmacology and the Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia
    N Engl J Med 351:560-74. 2004
    ..We examined the signaling pathway controlling the inhibitory effect of glucocorticoids on cell proliferation and interleukin-6 synthesis in bronchial smooth-muscle cells of subjects with asthma and those without asthma...
  18. ncbi Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia
    T Pabst
    Hematology Oncology Division, Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Nat Genet 27:263-70. 2001
    ..Ours is the first report of CEBPA mutations in human neoplasia, and such mutations are likely to induce the differentiation block found in AML...
  19. ncbi MicroRNA expression profiling in relation to the genetic heterogeneity of acute myeloid leukemia
    Mojca Jongen-Lavrencic
    Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands
    Blood 111:5078-85. 2008
    ..MicroRNA expression apparently bears specific relationships to the heterogeneous pathobiology of AML. Distinctive microRNA signatures appear of potential value in the clinical diagnosis of AML...
  20. ncbi Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma
    L Xu
    Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai
    Cancer Res 61:3176-81. 2001
    ..The expression profiling thus suggested multiple regulatory pathways involved in HCC, especially that related to LETFs...
  21. ncbi Characterization of CEBPA mutations in acute myeloid leukemia: most patients with CEBPA mutations have biallelic mutations and show a distinct immunophenotype of the leukemic cells
    Liang In Lin
    Graduate Institute of Medical Technology, National Taiwan University, Taipei, Taiwan
    Clin Cancer Res 11:1372-9. 2005
    ..We sought to characterize the CEBPA mutation in acute myeloid leukemia (AML) and to clarify if there is a distinct immunophenotype for leukemic cells with the mutation...
  22. ncbi Granulocyte inducer C/EBPalpha inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions
    Venkateshwar A Reddy
    Department of Medicine III, Ludwig Maximilians University and GSF National Research Center for Environment and Health, Munich, Germany
    Blood 100:483-90. 2002
    ..The functional blocking of PU.1 by C/EBPalpha could be the mechanism by which C/EBPalpha inhibits cell fates specified by PU.1 and directs cell development to the granulocyte lineage...
  23. ncbi Diminished expression of C/EBPalpha in skin carcinomas is linked to oncogenic Ras and reexpression of C/EBPalpha in carcinoma cells inhibits proliferation
    Minsub Shim
    Cell Signaling and Cancer Group, Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695 7633, USA
    Cancer Res 65:861-7. 2005
    ..These results indicate that oncogenic Ras negatively regulates C/EBPalpha expression and the loss of C/EBPalpha expression may contribute to the development of skin SCCs...
  24. ncbi Discovery of epigenetically masked tumor suppressor genes in endometrial cancer
    Noriyuki Takai
    Division of Hematology Oncology, Cedars Sinai Medical Center University of California at Los Angeles School of Medicine, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA
    Mol Cancer Res 3:261-9. 2005
    ..Our data suggest that C/ebpalpha and Tig1 function as tumor suppressor proteins in endometrial cancers and that their reexpression may be a therapeutic target...
  25. ncbi CCAAT/enhancer binding protein alpha assembles essential cooperating factors in common subnuclear domains
    F Schaufele
    Metabolic Research Unit and Department of Medicine, University of California, San Francisco, California 94143 0540, USA
    Mol Endocrinol 15:1665-76. 2001
    ..It also identifies an organizational activity, which we label "intranuclear marshalling," that may regulate gene expression by determining the cooperative and antagonistic interactions available at specific nuclear sites...
  26. pmc Sumoylation and the function of CCAAT enhancer binding protein alpha (C/EBP alpha)
    Arati Khanna-Gupta
    Division of Hematology, The Brigham and Women s Hospital, Harvard Medical School, Karp Building RB5 212, 1 Blackfan Circle, Boston, MA 02115, USA
    Blood Cells Mol Dis 41:77-81. 2008
    b>CCAAT enhancer binding protein alpha (C/EBP alpha) is the founding member of a family of basic region/leucine zipper (bZIP) transcription factors and is a master regulator of granulopoiesis...
  27. ncbi Conserved amino acids within CCAAT enhancer-binding proteins (C/EBP(alpha) and beta) regulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression
    Luis A Jurado
    Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Biol Chem 277:27606-12. 2002
    ..Our results indicate that both C/EBP proteins and CBP participate in the regulation of PEPCK gene transcription by thyroid hormone...
  28. ncbi Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations
    Masao Mizuki
    Department of Medicine, Hematology Oncology, University of Munster, Germany
    Blood 101:3164-73. 2003
    ..The identification of the transcriptional program induced by ITD mutations should ease the development of specific therapies...
  29. pmc Dephosphorylated C/EBPalpha accelerates cell proliferation through sequestering retinoblastoma protein
    Guo Li Wang
    Department of Pathology and Huffington Center on Aging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell Biol 25:1325-38. 2005
    ..Our data demonstrate a new pathway by which the phosphorylation-dependent switch of biological functions of C/EBPalpha promotes liver proliferation...
  30. pmc C/EBPalpha is a DNA damage-inducible p53-regulated mediator of the G1 checkpoint in keratinocytes
    Kyungsil Yoon
    Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695 7633, USA
    Mol Cell Biol 24:10650-60. 2004
    ..Our results uncover a novel role for C/EBPalpha as a p53-regulated DNA damage-inducible gene that has a critical function in the DNA damage G(1) checkpoint response in keratinocytes...
  31. ncbi The CCAAT enhancer-binding protein-alpha negatively regulates the transactivation of androgen receptor in prostate cancer cells
    Soma Chattopadhyay
    Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500 757, Republic of Korea
    Mol Endocrinol 20:984-95. 2006
    ..Our results suggest C/EBPalpha as a potent AR corepressor and provide insight into the role of C/EBPalpha in prostate development and cancer...
  32. ncbi C/EBPalpha mutations in acute myeloid leukaemias
    Claus Nerlov
    Mouse Biology Programme, European Molecular Biology Laboratory, Via Ramarini 32, 00016 Monterotondo, Italy
    Nat Rev Cancer 4:394-400. 2004
  33. ncbi Favorable prognostic significance of CEBPA mutations in patients with de novo acute myeloid leukemia: a study from the Acute Leukemia French Association (ALFA)
    Claude Preudhomme
    Département d Hématologie and INSERM U524, Hopital Claude Huriez, Lille, France
    Blood 100:2717-23. 2002
    ..We thus propose a risk classification that includes in the favorable subgroup all patients from the intermediate subgroup displaying CEBPA mutations when not associated with FLT3-ITD...
  34. ncbi Insulin-induced up-regulated uncoupling protein-1 expression is mediated by insulin receptor substrate 1 through the phosphatidylinositol 3-kinase/Akt signaling pathway in fetal brown adipocytes
    Angela M Valverde
    Departamento de Bioquimica y Biologia Molecular, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad Complutense de Madrid, Facultad de Farmacia, Universidad Complutense, Spain
    J Biol Chem 278:10221-31. 2003
    ..These data provide strong evidence for an essential role of IRS-1 through the PI 3-kinase/Akt signaling pathway inducing UCP-1 gene expression by insulin...
  35. ncbi Induction of C/EBPalpha activity alters gene expression and differentiation of human CD34+ cells
    Jorg Cammenga
    Laboratory of Molecular Aspects of Hematopoiesis, Sloan Kettering Institute, Division of Hematologic Oncology, Memorial Sloan Kettering Cancer Center MSKCC, New York, NY 10021, USA
    Blood 101:2206-14. 2003
    ..Given the known differences in murine and human promoter regulatory sequences, this inducible system allows the identification of transcription factor target genes in a physiologic, human hematopoietic progenitor cell background...
  36. ncbi Epigenetic modulation of tumor suppressor CCAAT/enhancer binding protein alpha activity in lung cancer
    Yasuhiro Tada
    Division of Human Cancer Genetics, Department of Molecular Virology, Immunology, and Medical Genetics, The Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
    J Natl Cancer Inst 98:396-406. 2006
    ..We hypothesized that DNA methylation and histone acetylation of the C/EBPalpha promoter may modulate C/EBPalpha expression in lung cancer...
  37. ncbi Alcohol metabolism-mediated oxidative stress down-regulates hepcidin transcription and leads to increased duodenal iron transporter expression
    Duygu Dee Harrison-Findik
    Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198 5820, USA
    J Biol Chem 281:22974-82. 2006
    ..Collectively, these findings indicate that alcohol metabolism-mediated oxidative stress regulates hepcidin transcription via C/EBPalpha, which in turn leads to increased duodenal iron transport...
  38. ncbi Tumor suppressor activity of CCAAT/enhancer binding protein alpha is epigenetically down-regulated in head and neck squamous cell carcinoma
    Kristi L Bennett
    Department of Molecular Genetics, The Comprehensive Cancer Center, Ohio State University, Columbus, Ohio, USA
    Cancer Res 67:4657-64. 2007
    Tumor suppressor CCAAT enhancer binding protein alpha (C/EBPalpha) is a transcription factor involved in cell cycle control and cellular differentiation...
  39. ncbi Modeling of C/EBPalpha mutant acute myeloid leukemia reveals a common expression signature of committed myeloid leukemia-initiating cells
    Peggy Kirstetter
    European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo 00016, Italy
    Cancer Cell 13:299-310. 2008
    ..Expression profiling of this population against normal Mac1+c-Kit+ progenitors revealed a signature shared with MLL-AF9-transformed AML...
  40. ncbi Association between altered expression of adipogenic factor SREBP1 in lipoatrophic adipose tissue from HIV-1-infected patients and abnormal adipocyte differentiation and insulin resistance
    Jean Philippe Bastard
    INSERM Research Unit 402, Faculty of Medicine Saint Antoine, Paris, France
    Lancet 359:1026-31. 2002
    ..In-vitro studies show that HIV-1-protease inhibitors affect adipocyte differentiation at an early step involving sterol-regulatory-element-binding-protein-1 (SREBP1), but in-vivo studies are lacking...
  41. ncbi Biallelic mutations in the CEBPA gene and low CEBPA expression levels as prognostic markers in intermediate-risk AML
    Sahar Barjesteh van Waalwijk van Doorn-Khosrovani
    Institute of Hematology, Erasmus Medical Centre, Rotterdam, The Netherlands
    Hematol J 4:31-40. 2003
    ..By including this newly developed PCR assay, we define a subgroup of good-risk patients within the heterogeneous intermediate-risk group of AML...
  42. pmc Respiratory failure due to differentiation arrest and expansion of alveolar cells following lung-specific loss of the transcription factor C/EBPalpha in mice
    Daniela S Bassères
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 26:1109-23. 2006
    The leucine zipper family transcription factor CCAAT enhancer binding protein alpha (C/EBPalpha) inhibits proliferation and promotes differentiation in various cell types...
  43. ncbi Acetylation and deacetylation regulate CCAAT/enhancer binding protein beta at K39 in mediating gene transcription
    TERESA I CESENA
    Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI 48109 5622, United States
    Mol Cell Endocrinol 289:94-101. 2008
    ..These findings suggest that acetylation of C/EBPbeta at K39 is an important and dynamic regulatory event that contributes to its ability to transactivate target genes, including those associated with adipogenesis and adipocyte function...
  44. ncbi Mutations of CEBPA in acute myeloid leukemia FAB types M1 and M2
    Jennifer Snaddon
    Cancer Research UK Medical Oncology Unit, Charterhouse Square, St Bartholomew s Hospital, London, United Kingdom
    Genes Chromosomes Cancer 37:72-8. 2003
    ..In conclusion, mutation of CEBPA is a recurrent finding in AML and appears specific to the intermediate cytogenetic risk group patients...
  45. ncbi Mutations in the gene encoding the transcription factor CCAAT/enhancer binding protein alpha in myelodysplastic syndromes and acute myeloid leukemias
    Adrian F Gombart
    Cedars Sinai Medical Center, Burns and Allen Research Institute, Division of Hematology Oncology, University of California Los Angeles School of Medicine, 90048, USA
    Blood 99:1332-40. 2002
    ..These results suggest that mutation of C/EBPalpha is involved in specific subtypes of AML and in MDS, but may occur rarely in other types of leukemias or nonhematologic malignancies...
  46. ncbi The amino terminal and E2F interaction domains are critical for C/EBP alpha-mediated induction of granulopoietic development of hematopoietic cells
    Francesco D'alo'
    Hematology Oncology Division, Harvard Institutes of Medicine, Boston, MA 02115, USA
    Blood 102:3163-71. 2003
    ..These results further emphasize the importance of inhibition of proliferative pathways in granulopoiesis and demonstrate that several regions of the C/EBP alpha protein are involved in this mechanism...
  47. ncbi BCR-ABL suppresses C/EBPalpha expression through inhibitory action of hnRNP E2
    Danilo Perrotti
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Nat Genet 30:48-58. 2002
    ..Our results indicate that BCR-ABL regulates the expression of C/EBPalpha by inducing hnRNP E2-which inhibits the translation of CEBPA mRNA...
  48. ncbi C/EBPalphap30, a myeloid leukemia oncoprotein, limits G-CSF receptor expression but not terminal granulopoiesis via site-selective inhibition of C/EBP DNA binding
    Rebecca Cleaves
    Division of Pediatric Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Oncogene 23:716-25. 2004
    ..Variation in affinity for selected cis elements among isoforms may affect the biology of basic region-leucine zipper (bZIP) proteins...
  49. ncbi Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis
    Qi Qun Tang
    Biological Chemistry, Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 319:235-9. 2004
    ..g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPbeta, C/EBPalpha, and PPARgamma participate in a cascade during adipogenesis...
  50. ncbi McBindall--a better name for CCAAT/enhancer binding proteins?
    S L McKnight
    Department of Biochemistry, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 107:259-61. 2001
    ..Three new studies give evidence that this regulation is mediated by protein:protein interactions completely distinct from the role of C/EBPs in gene expression...
  51. pmc Stimulation of preadipocyte differentiation by steroid through targeting of an HDAC1 complex
    Nadine Wiper-Bergeron
    The Ottawa Health Research Institute, University of Ottawa, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9
    EMBO J 22:2135-45. 2003
    ..Recapitulation of the glucocorticoid effect by progestin treatment in the presence of the progesterone receptor ligand-binding domain suggests a conserved mechanism relevant to many aspects of steroid-mediated differentiation...
  52. ncbi Internal tandem duplication mutation of FLT3 blocks myeloid differentiation through suppression of C/EBPalpha expression
    Rui Zheng
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Blood 103:1883-90. 2004
    ..Forced expression of C/EBPalpha was also able to overcome FLT3/ITD-mediated differentiation block, further proving the importance of C/EBPalpha in this process...
  53. ncbi CEBPalpha mutations in childhood acute myeloid leukemia
    D C Liang
    Division of Pediatric Hematology Oncology, Mackay Memorial Hospital, Taipei, Taiwan
    Leukemia 19:410-4. 2005
    ..Our results showed that CEBPalpha mutations occurred in 6% of childhood AML and most exhibited combined mutations in both N-terminal part and bZIP domain...
  54. ncbi CBFB-SMMHC is correlated with increased calreticulin expression and suppresses the granulocytic differentiation factor CEBPA in AML with inv(16)
    Daniel Helbling
    Department of Clinical Research, University Hospital, Berne, Switzerland
    Blood 106:1369-75. 2005
    ..Inhibition of calreticulin by siRNA restored CEBPA levels. Our results suggest that modulation of CEBPA by calreticulin represents a novel mechanism involved in the differentiation block in CBFB-SMMHC AML...
  55. ncbi CCAAT/enhancer-binding protein (C/EBP) beta is acetylated at multiple lysines: acetylation of C/EBPbeta at lysine 39 modulates its ability to activate transcription
    TERESA I CESENA
    Cellular and Molecular Biology Program, Department of Obstetrics Gynecology, University of Michigan, Ann Arbor 48109, USA
    J Biol Chem 282:956-67. 2007
    ..These data suggest that acetylation of Lys-39 of C/EBPbeta, alone or in combination with acetylation at other lysines, may play a role in C/EBPbeta-mediated transcriptional activation...
  56. ncbi Molecular regulation of the PAI-1 gene by hypoxia: contributions of Egr-1, HIF-1alpha, and C/EBPalpha
    Hui Liao
    Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    FASEB J 21:935-49. 2007
    ..These studies are relevant to ischemic up-regulation of the PAI-1 gene and consequent accrual of microvascular thrombus under ischemic conditions...
  57. ncbi Calcineurin mediates the calcium-dependent inhibition of adipocyte differentiation in 3T3-L1 cells
    Joel W Neal
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago, IL 60611, USA
    J Biol Chem 277:49776-81. 2002
    ....
  58. ncbi C/EBPalpha regulates human adiponectin gene transcription through an intronic enhancer
    Liping Qiao
    Graduate Center for Nutritional Sciences, University of Kentucky, 900 S Limestone, Lexington, KY 40536, USA
    Diabetes 54:1744-54. 2005
    ..Thus, C/EBPalpha is a key transcription factor for full activation of human adiponectin gene transcription in mature adipocytes through interaction with response elements in the intronic enhancer...
  59. ncbi Proteomic identification of the MYST domain histone acetyltransferase TIP60 (HTATIP) as a co-activator of the myeloid transcription factor C/EBPalpha
    D Bararia
    Department of Medicine III, University of Munich and Clinical Cooperative Group, HelmholtzZentrum German Research Center for Environmental Health, Munich, Germany
    Leukemia 22:800-7. 2008
    ..These findings point to a functional synergism between C/EBPalpha and HTATIP in myeloid differentiation and suggest that HTATIP might be an important player in leukemogenesis...
  60. ncbi ROS mediate the hypoxic repression of the hepcidin gene by inhibiting C/EBPalpha and STAT-3
    Si On Choi
    Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 799, Republic of Korea
    Biochem Biophys Res Commun 356:312-7. 2007
    ..These results suggest that ROS repress the hepcidin gene by preventing C/EBPalpha and STAT-3 binding to hepcidin promoter during hypoxia...
  61. ncbi Reprogramming of committed T cell progenitors to macrophages and dendritic cells by C/EBP alpha and PU.1 transcription factors
    Catherine V Laiosa
    Department of Developmental and Molecular Biology and the Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Immunity 25:731-44. 2006
    ..1 in multilineage precursors...
  62. ncbi Adiponectin gene activation by thiazolidinediones requires PPAR gamma 2, but not C/EBP alpha-evidence for differential regulation of the aP2 and adiponectin genes
    Birgit Gustafson
    Department of Internal Medicine, The Lundberg Laboratory for Diabetes Research, The Sahlgrenska Academy at Goteborg University, Goteborg SE 413 45, Sweden
    Biochem Biophys Res Commun 308:933-9. 2003
    ..These data show that activation of the adiponectin gene can be separated from effects on adipogenic genes...
  63. pmc Myeloid lineage switch of Pax5 mutant but not wild-type B cell progenitors by C/EBPalpha and GATA factors
    Barry Heavey
    Research Institute of Molecular Pathology, Vienna Biocenter, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    EMBO J 22:3887-97. 2003
    ..The same regulators were unable to induce a myeloid lineage switch in Pax5(+/+) pro-B cells, indicating that Pax5 dominates over myeloid transcription factors in B-lymphocytes...
  64. ncbi Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction
    Janki Rangatia
    Medicine III at University of Munich Hospital Grosshadern and GSF Research Center, Munich, Germany
    Oncogene 22:4760-4. 2003
    ....
  65. pmc Erythropoietin mediates hepcidin expression in hepatocytes through EPOR signaling and regulation of C/EBPalpha
    Jorge P Pinto
    Iron Genes and Immune System, Instituto de Biologia Molecular e Celular, University of Porto, Porto, Portugal
    Blood 111:5727-33. 2008
    ....
  66. ncbi Phosphorylation of the large subunit of replication factor C is associated with adipocyte differentiation
    Min Jung Park
    Department of Molecular Biology, Pusan National University, Jang Jeon Dong, Keum Jeong Gu, Busan 607 935, Korea
    FEBS J 274:1235-45. 2007
    ....
  67. ncbi The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest
    Christine Muller
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    J Biol Chem 279:7353-8. 2004
    ....
  68. ncbi Depletion of cAMP-response element-binding protein/ATF1 inhibits adipogenic conversion of 3T3-L1 cells ectopically expressing CCAAT/enhancer-binding protein (C/EBP) alpha, C/EBP beta, or PPAR gamma 2
    Keith E Fox
    Research Service, Veterans Affairs Medical Center, University of Colorado Health Sciences Center, Denver, Colorado 80220, USA
    J Biol Chem 281:40341-53. 2006
    ..The data also indicate that CREB not only functions during the initiation of adipogenic conversion but also at later stages...
  69. pmc A protein encoded by the bovine herpesvirus 1 latency-related gene interacts with specific cellular regulatory proteins, including CCAAT enhancer binding protein alpha
    Florencia Meyer
    Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68503, USA
    J Virol 81:59-67. 2007
    ..LR fusion protein interacts with two proteins that can induce apoptosis (Bid and Cdc42) and with CCAAT enhancer binding protein alpha (C/EBP-alpha)...
  70. pmc Essential requirement of CCAAT/enhancer binding proteins in embryogenesis
    Valerie Begay
    Max Delbrueck Center for Molecular Medicine, Robert Roessle Str 10, 13092 Berlin, Germany
    Mol Cell Biol 24:9744-51. 2004
    ..Our data thus reveal novel essential, redundant, and dosage dependent functions of C/EBPs...
  71. ncbi The transcriptional basis of adipocyte development
    Evan D Rosen
    Division of Endocrinology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Prostaglandins Leukot Essent Fatty Acids 73:31-4. 2005
    ..These approaches will be discussed, along with the roles of some new transcriptional players in adipogenesis, including the O/E family of proteins...
  72. ncbi Subnuclear targeting of Runx1 is required for synergistic activation of the myeloid specific M-CSF receptor promoter by PU.1
    Xiangen Li
    Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Biochem 96:795-809. 2005
    ..1. Taken together, our findings establish that subnuclear targeting of Runx1 is a critical component of myeloid-specific transcriptional control...
  73. ncbi CCAAT/enhancer binding protein alpha (C/EBPalpha) and C/EBPalpha myeloid oncoproteins induce bcl-2 via interaction of their basic regions with nuclear factor-kappaB p50
    Ido Paz-Priel
    Division of Pediatric Oncology, Johns Hopkins University, CRB 253, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Mol Cancer Res 3:585-96. 2005
    ..Targeting interaction between C/EBPalpha basic region and NF-kappaB p50 may contribute to the therapy of AML and other malignancies expressing C/EBPs...
  74. ncbi C/EBPalpha and the pathophysiology of acute myeloid leukemia
    Beatrice U Mueller
    Department of Internal Medicine, University Hospital, Switzerland
    Curr Opin Hematol 13:7-14. 2006
    ..The finding of C/EBPalpha alterations in subgroups of acute myeloid leukemia patients suggests a direct link between critically decreased C/EBPalpha function and the development of the disorder...
  75. ncbi C/EBPalpha: a tumour suppressor in multiple tissues?
    Mikkel Bruhn Schuster
    Section for Gene Therapy Research, Department of Clinical Biochemistry, Copenhagen University Hospital, Juliane Maries Vej 20 9322, DK2100 Copenhagen, Denmark
    Biochim Biophys Acta 1766:88-103. 2006
    ..Here, we will review these data and probe the possibility that C/EBPalpha also act as a tumour suppressor in other C/EBPalpha-expressing tissues...
  76. pmc CDDO induces granulocytic differentiation of myeloid leukemic blasts through translational up-regulation of p42 CCAAT enhancer binding protein alpha
    Steffen Koschmieder
    Department of Medicine, Hematology and Oncology, University of Munster, Münster Germany
    Blood 110:3695-705. 2007
    ..Because AML is characterized by arrested differentiation, our data suggest the inclusion of CDDO in the therapy of AML characterized by dysfunctional CEBPA expression...
  77. pmc C/EBPalpha directs monocytic commitment of primary myeloid progenitors
    Dehua Wang
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21231, USA
    Blood 108:1223-9. 2006
    ..A C/EBPalpha variant incapable of zippering with c-Jun did not induce monopoiesis, and a variant unable to bind NF-kappaB p50 stimulated granulopoiesis, suggesting their cooperation with C/EBPalpha during monocytic commitment...
  78. pmc Comprehensive analysis of myeloid lineage conversion using mice expressing an inducible form of C/EBP alpha
    Yumi Fukuchi
    Division of Cellular Therapy, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    EMBO J 25:3398-410. 2006
    ..Our study reveals that C/EBPalpha can activate the latent myeloid differentiation program of MEP and CLP and shows that its global activation affects multilineage homeostasis in vivo...
  79. ncbi C/EBPalpha deficiency results in hyperproliferation of hematopoietic progenitor cells and disrupts macrophage development in vitro and in vivo
    Victoria Heath
    Laboratory of Protein Dynamics and Signaling, Science Applications International Corporation Frederick, Inc, Frederick, MD 20702 1201, USA
    Blood 104:1639-47. 2004
    ..These findings show that C/EBPalpha deficiency results in hyperproliferation of HPCs and a block in the ability of multipotential progenitors to differentiate into bipotential granulocyte/macrophage progenitors and their progeny...
  80. pmc MicroRNA-661, a c/EBPalpha target, inhibits metastatic tumor antigen 1 and regulates its functions
    Sirigiri Divijendra Natha Reddy
    Department of Biochemistry and Molecular Biology and Institute of Coregulator Biology, The George Washington University Medical Center, Washington, District of Columbia 20037, USA
    Cancer Res 69:5639-42. 2009
    ..Based on these findings, we suggest that miR-661 be further investigated for therapeutic use in down-regulating the expression of MTA1 in cancer cells...
  81. ncbi p300 coactivates the adipogenic transcription factor CCAAT/enhancer-binding protein alpha
    R L Erickson
    Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0622, USA
    J Biol Chem 276:16348-55. 2001
    ..Analyses using C/EBPalpha conserved region mutants suggest that multiple regions (CR2 and CR3) of the C/EBPalpha transactivation domain functionally interact with p300...
  82. pmc Analysis of the Xenopus laevis CCAAT-enhancer binding protein alpha gene promoter demonstrates species-specific differences in the mechanisms for both auto-activation and regulation by Sp1
    F T Kockar
    Cardiff School of Biosciences, Cardiff University, Museum Avenue, PO Box 911, Cardiff CF10 3US, UK
    Nucleic Acids Res 29:362-72. 2001
    ..In addition, we demonstrate a novel species-specific action of Sp1 in the regulation of C/EBPalpha expression, with the factor able to repress the murine promoter but activate the XENOPUS: gene...
  83. ncbi AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia
    T Pabst
    Hematology Oncology Division, Harvard Institutes of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 7:444-51. 2001
    ..Conditional expression of C/EBPalpha in AML1-ETO-positive Kasumi-1 cells results in neutrophilic differentiation. We suggest that restoring C/EBPalpha expression will have therapeutic implications in RUNX1-CBF2T1-positive leukemias...
  84. ncbi C/EBPalpha regulates hepatic transcription of hepcidin, an antimicrobial peptide and regulator of iron metabolism. Cross-talk between C/EBP pathway and iron metabolism
    Brice Courselaud
    INSERM U522, CHRU Pontchaillou, 35033 Rennes Cedex, France
    J Biol Chem 277:41163-70. 2002
    ..Taken together, these data demonstrate that C/EBPalpha is likely to be a key regulator of HEPC gene transcription and provide a novel mechanism for cross-talk between the C/EBP pathway and iron metabolism...
  85. ncbi Induction of granulocytic differentiation by 2 pathways
    Pu Zhang
    Harvard Institutes of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Blood 99:4406-12. 2002
    The CCAAT enhancer binding protein alpha (C/EBP alpha) transcription factor plays a critical role in granulocytopoiesis...
  86. ncbi Reciprocal effects of C/EBPalpha and PKCdelta on JunB expression and monocytic differentiation depend upon the C/EBPalpha basic region
    Huaitian Liu
    Division of Pediatric Oncology, Johns Hopkins University, Baltimore, MD, USA
    Blood 101:3885-92. 2003
    ..Exogenous JunB restored AP-1 DNA-binding but did not prevent inhibition of macrosialin expression by C/EBPalpha-ER, indicating that JunB is not the only target relevant to inhibition of monopoiesis by C/EBPalpha...
  87. pmc Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during adipogenesis
    Q Q Tang
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21209, USA
    Proc Natl Acad Sci U S A 97:12446-50. 2000
    ..In support of these findings, up-regulation of CHOP-10 with the protease inhibitor N-acetyl-Leu-Leu-norleucinal prevents activation of C/EBPbeta, expression of C/EBPalpha, and adipogenesis...
  88. ncbi Sonic hedgehog increases the commitment of pluripotent mesenchymal cells into the osteoblastic lineage and abolishes adipocytic differentiation
    S Spinella-Jaegle
    Bone Diseases Group, Department of Biotechnology, Hoechst-Marion-Roussel, 111 route de Noisy, 93230 Romainville, France
    J Cell Sci 114:2085-94. 2001
    ..Given the inverse relationship between marrow adipocytes and osteoblasts with aging, agonists of the Hh signaling pathway might constitute potential drugs for preventing and/or treating osteopenic disorders...
  89. ncbi Activin A inhibits differentiation of 3T3-L1 preadipocyte
    Shizuka Hirai
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto shi 606 8502, Japan
    Mol Cell Endocrinol 232:21-6. 2005
    ..On the other hand, activin A treatment had no effect on the mitotic clonal expansion. These results indicate that activin A inhibits adipogenesis via affecting the transcriptional factor cascade upstream of PPARgamma expression...
  90. ncbi Modulation of early human preadipocyte differentiation by glucocorticoids
    Julianna J Tomlinson
    Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9
    Endocrinology 147:5284-93. 2006
    ....
  91. ncbi C/EBPalpha-dependent induction of glutathione S-transferase zeta/maleylacetoacetate isomerase (GSTzeta/MAAI) expression during the differentiation of mouse fibroblasts into adipocytes
    Li Qiang
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem Biophys Res Commun 340:845-51. 2006
    ..These data suggest a role for GSTzeta/MAAI in mature adipocytes that may be responsive to the thiazolidinedione class of insulin sensitizing PPARgamma ligands...
  92. ncbi Overexpression of uncoupling protein 4 promotes proliferation and inhibits apoptosis and differentiation of preadipocytes
    Min Zhang
    Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, 210004 Nanjing, China
    Life Sci 79:1428-35. 2006
    ..markers such as fatty acid synthetase, peroxisome proliferator-activated receptor gamma, CCAAT enhancer binding protein alpha, adipocyte lipid binding protein and lipoprotein lipase were downregulated...
  93. ncbi Impaired lipid accumulation by trans10, cis12 CLA during adipocyte differentiation is dependent on timing and length of treatment
    Linda Granlund
    Institute of Basic Medical Sciences, Department of Nutrition, University of Oslo, N 0316 Oslo, Norway
    Biochim Biophys Acta 1687:11-22. 2005
    ....
  94. ncbi Prostaglandin F2alpha inhibits adipocyte differentiation via a G alpha q-calcium-calcineurin-dependent signaling pathway
    Li Liu
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, Illinois 60611, USA
    J Cell Biochem 100:161-73. 2007
    ....
  95. ncbi Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway
    Cheng Huang
    Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 294 Tai Yuan Road, Shanghai 200031, China
    Biochem Biophys Res Commun 348:571-8. 2006
    ..These studies suggest that BBR works on multiple molecular targets as an inhibitor of PPARgamma and alpha, and is a potential weight reducing, hypolipidemic, and hypoglycemic drug...
  96. pmc Critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade
    Maria A Jimenez
    Division of Endocrinology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Mol Cell Biol 27:743-57. 2007
    ..Altogether, these data place Ebf1 within the known transcriptional cascade of adipogenesis and suggest critical roles for Ebf1 and Ebf2...
  97. ncbi Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts
    Sang Wan Kim
    Department of Internal Medicine, Seoul National University College of Medicine, 28 Yungun Dong, Chongno Gu, Seoul 110 744, Republic of Korea
    Biochem Biophys Res Commun 327:811-9. 2005
    ..These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes...
  98. pmc Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation
    Qiang Tong
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Ave, Bldg 1, Rm 207, Boston, MA 02115, USA
    Mol Cell Biol 25:706-15. 2005
    ..Thus, these results show that in addition to its previously recognized function in suppressing PPARgamma transcriptional activity, interaction of GATA factors with C/EBP is necessary for their ability to negatively regulate adipogenesis...
  99. pmc Decreased activity and enhanced nuclear export of CCAAT-enhancer-binding protein beta during inhibition of adipogenesis by ceramide
    Kam M Sprott
    Department of Pharmacology and Toxicology, University of Kansas, Lawrence, 5064 Malott Hall, KS 66045, USA
    Biochem J 365:181-91. 2002
    ....
  100. ncbi Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation
    Yuchang Fu
    Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294 3360, USA
    J Lipid Res 46:1369-79. 2005
    ....
  101. ncbi Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo
    Toshiyuki Asaki
    Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo, Kyoto 606 8501, Japan
    Mol Cell Endocrinol 218:119-28. 2004
    ..Unexpectedly, the transcriptional cascade of adipogenesis in vivo described here is distinct from the cascade in vitro previously reported...

Research Grants62

  1. Molecular mechanisms of C/EBP alpha mediated growth arrest
    Nikolai A Timchenko; Fiscal Year: 2013
    ..The elucidation of these mechanisms will provide basement for the generation of therapeutic approaches to prevent tumors. ..
  2. SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION
    Richard N Day; Fiscal Year: 2012
    ..Discovering how nuclear architecture controls gene expression will be the cornerstone for understanding how genomes work. ..
  3. Regulation of adipocyte differentiation and metabolism
    Ormond A MacDougald; Fiscal Year: 2010
    ....
  4. The Role of C/EBPa in Genome Stability and Tumor Progression
    JONATHAN RUSSELL HALL; Fiscal Year: 2012
    ..abstract_text> ..
  5. Role of C/EBPalpha in Cytoprotection and Recovery from Lung Injury
    Machiko Ikegami; Fiscal Year: 2012
    ..Furthermore, the molecular mechanisms, in particular the signaling pathways regulating Cebpa, of recombinant human FGF-7 treatment for acute lung injury will be determined. ..
  6. Role of Age in Liver Cancer
    Nikolai A Timchenko; Fiscal Year: 2013
    ..Thus, the proposed studies will provide novel gankyrin-based approaches for the prevention of liver cancer. ..
  7. Targeting the C/EBPalpha-Gfi-pathway in CML stem cells
    Bruno Calabretta; Fiscal Year: 2013
    ....
  8. Regulation of CYP2B6 in Human Liver
    Hongbing Wang; Fiscal Year: 2013
    ....
  9. METABOLIC HOMEOSTASIS IN LIVER REGENERATION
    LINDA GREENBAUM; Fiscal Year: 1999
    ....
  10. Transcription & Function of Adipocyte Beta3AR in Obesity
    Sheila Collins; Fiscal Year: 2006
    ..abstract_text> ..
  11. CDP/CUT AND MYELOID DIFFERENTIATION
    Nancy Berliner; Fiscal Year: 2001
    ..More recently, we have established that the transcriptional regulator CCAAT enhancer binding protein alpha (C/EBPa), a transcriptional activator known to upregulate expression of many myeloid genes, binds ..
  12. Mechanisms of Testosterone Effects on Body Composition
    Shalendar Bhasin; Fiscal Year: 2007
    ..abstract_text> ..
  13. Antiadipogenic Influence of HIV Protease Inhibitors
    M Lane; Fiscal Year: 2005
    ..The long term objective of the proposed research is to facilitate the design of anti-HIV therapeutics that lack lipoatrophic potential. ..
  14. REGULATION OF TRANSTHYRETIN IN THE LIVER
    Pradip Raychaudhuri; Fiscal Year: 2006
    ..abstract_text> ..
  15. C/EBP ALPHA MEDIATED REGULATION OF P21/SDI-1
    Nikolai Timchenko; Fiscal Year: 2001
    ..For this goal we will clone the genes that are responsible for C/EBPalpha-mediated growth arrest. ..
  16. CYTOPLASMIC FACTORS IN CELLULAR GROWTH
    NANCY BUCHER; Fiscal Year: 1999
    ..These new approaches should allow basic physiological and molecular biological observations to be integrated into a more comprehensive formulation of how liver regeneration operates. ..
  17. The role of calcineurin in adipocyte biology
    Neil Clipstone; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  18. C/EBP ALPHA REGULATION OF ACUTE PHASE RESPONSE IN VIVO
    Gretchen Darlington; Fiscal Year: 2004
    ..In aim 4, the biological consequences of the truncated isoforms of C/EBPbeta will be examined in transgenic and cell culture models. ..
  19. EFFECT OF FAT DEPOT ORIGIN ON PREADIPOCYTE FUNCTION
    James Kirkland; Fiscal Year: 2004
    ..These studies will point to potential future strategies for altering function in specific fat depots. ..
  20. C/EBPalpha-mediated therapy for acute myeloid leukemia
    Hanna Radomska; Fiscal Year: 2004
    ..Nevertheless, completion of the proposed career development program will further expand her research experience and will enable her to pursue an independent research career. ..
  21. C/EBP AND ALVEOLAR TYPE II CELL DIFFERENTIATION
    SHELDON FEINSTEIN; Fiscal Year: 2000
    ....
  22. MYELOID TRANSCRIPTION FACTORS AND LEUKEMIA
    Erik Nelson; Fiscal Year: 2000
    ..Our goal from these studies is to make a correlation between the structure of the C/EBPalpha protein and its function in myelopoiesis and leukemogenesis. ..
  23. DEREGULATION OF MYELOPOIESIS BY ZINC FINGER PROTEIN EVI1
    Archibald Perkins; Fiscal Year: 2004
    ..abstract_text> ..
  24. HORMONAL CONTROL OF ADIPOSE GENE EXPRESSION
    M Lane; Fiscal Year: 2007
    ..The 32kDa CUP/AP-2alpha- interacting protein, p32, previously identified in our lab, will be characterized and its role determined. ..
  25. MECHANISM OF REPRESSION OF ADIPOGENESIS
    Tamara Otto; Fiscal Year: 2004
    ..abstract_text> ..
  26. Developmental Checkpoints of Murine Mast Cells
    Koichi Akashi; Fiscal Year: 2009
    ..Results obtained from this project will be critical to study mast cell biology, which eventually will help develop new therapeutic strategies for a variety of allergic and autoimmune disorders. ..
  27. Identification of Regulatory Elements of the Human C/EBPalpha Gene
    KAREN O BRIEN; Fiscal Year: 2006
    ..Second, we will determine whether YY1, PU.1, or other proteins regulate C/EBPa through the ORE during granulopoiesis. ..
  28. C/EBP ALPHA REGULATION OF ADIPOSE TISSUE DIFFERENTIATION
    Gretchen Darlington; Fiscal Year: 2000
    ..The resulting information would be an initial step in developing strategies to activate C/EBPa prematurely or to regulate its expression in adipose tissues. ..
  29. Murine models of activated FLT3 receptor tyrosine kinase
    Benjamin Lee; Fiscal Year: 2007
    ....
  30. MOLECULAR CONTROL OF MAMMALIAN ENERGY HOMEOSTASIS
    STEVEN MCKNIGHT; Fiscal Year: 2001
    ....
  31. CELL SURVIVAL AND CANCER
    Bruno Calabretta; Fiscal Year: 2007
    ..The long-term objective of this Program Project is to utilize this information as a molecular basis for devising more rational approaches to anti-cancer therapies. ..
  32. Constraint of Myeloid Lineage Potential of Early T Progenitors by Hes1
    James Bell; Fiscal Year: 2009
    ....
  33. PERINATAL STUDIES OF DISORDERS OF FETAL METABOLISM
    Satish Kalhan; Fiscal Year: 2002
    ..These studies are likely to contribute to our understanding of metabolic influences on fetal and neonatal growth, on maternal metabolism and health, and may develop intervention strategies for disease states. ..
  34. C/EBP alpha in Aging Liver
    Nikolai Timchenko; Fiscal Year: 2009
    ..Based on data obtained in this project, we are planning to start developing a strategy to correct liver proliferation in elderly. ..
  35. Role of C/EBPalpha in MDS and MDS Progression
    Alan Friedman; Fiscal Year: 2008
    ..These data will be useful for interpreting expression data from human MDS patients and may uncover a common pathway leading to inhibition of apoptosis and progression to AML. ..
  36. Cell Cycle Regulation and Leukemogenesis by CBFb-SMMHC
    Alan Friedman; Fiscal Year: 2007
    ....
  37. Conference:Lung Surfactant:Cellular & Molecular Biology
    Robert Mason; Fiscal Year: 2002
    ..abstract_text> ..
  38. A TRANSCRIPTIONAL PROGRAM REGULATING MYELOPOIESIS
    Alan Friedman; Fiscal Year: 2004
    ..Myelopoiesis at various levels of C/EBP activity and progenitor fate after various times of C/EBP inhibition will be assessed. ..
  39. Regional Differences in Preadipocyte Development
    James Kirkland; Fiscal Year: 2007
    ..These studies will elucidate cell dynamic and developmental mechanisms predisposing preadipocytes from different fat depots to acquire distinct characteristics responsible for regional obesity and the metabolic syndrome. ..
  40. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2010
    ..Together, these studies will provide new insights into the stress-induced regulation of lipogenesis in adipocytes, a potentially important avenue for modulating energy balance. ..
  41. AAV GENE THERAPY OF STEM CELLS AND MACROPHAGES
    Daniel Tenen; Fiscal Year: 2001
    ....
  42. Targeting a reversibly drug tolerant cancer cell subpopulation
    Marie K Classon; Fiscal Year: 2010
    ..By studying the mechanisms that underlie this state, we expect to be able to develop effective therapeutic strategies to disrupt drug tolerance, thereby improving the efficacy of cancer drugs. ..
  43. The Role of pRB Family Members in Ras-mediated Tumorigenesis
    Marie K Classon; Fiscal Year: 2010
    ..Together, these studies are expected to provide significant clues regarding the mechanism for the observed functional differences among these closely related proteins in tumorogenesis. ..
  44. HORMONAL SIGNALING AND PREADIPOCYTE DIFFERENTIATION
    STEPHEN ROBERT FARMER; Fiscal Year: 2010
    ....
  45. Transcriptional Control of Respiratory Epithelial Progenitor Cells
    Jeffrey A Whitsett; Fiscal Year: 2010
    ..A Stem Cell Consortium will be established at CCHMC, Univ. of Cincinnati College of Medicine to develop education, training, and research bridging hematopoietic stem cell and pulmonary stem cell biology on our campus. ..
  46. FACTORS INFLUENCING STEM CELL AND PLATELET PRODUCTION
    Malcolm Moore; Fiscal Year: 2002
    ..e., adult populations acquiring features of neonatal cells, or whether there is a tendency for "immortalized" cells to undergo neoplastic transformation. ..
  47. UCSF High Throughput Microscope
    Fred Schaufele; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  48. Targeting the CaM Kinases in Treating Myeloid Leukemia
    Steven Collins; Fiscal Year: 2009
    ....
  49. Ribonucleotide Reductase Antisense Strategy in AML
    Guido Marcucci; Fiscal Year: 2004
    ..It is expected that the analysis of these data will allow us to elucidate the intrinsic mechanisms of the antitumor activity of the antisense compounds and plan their incorporation in future therapeutic strategies for AML patients. ..
  50. Therapeutic effect of boswellin in prostate cancer
    Yongkui Jing; Fiscal Year: 2005
    ..We hope our proposal will lead to the discovery of a novel complementary agent for prostate cancer treatment. ..
  51. MOLECULAR CONTROL OF ADIPOGENESIS AND OBESITY
    Stephen Farmer; Fiscal Year: 2004
    ....
  52. BCI2 Antisense Against Chemoresistance in Elderly AML
    Guido Marcucci; Fiscal Year: 2003
    ..It is expected that the analysis of the clinical and correlative laboratory results will support testing of this combination in a phase III setting. ..
  53. REGULATION OF THE HUMAN CD34 STEM CELL GENE
    Daniel Tenen; Fiscal Year: 2007
    ..1 To use CD34 regulatory elements expressing Cre recombinase and the tetracycline transactivator to enable tissue specific, regulatable expression of heterologous genes in the stem cell compartment. ..
  54. Regulatory Role of Growth Hormone Secretagogue Receptor
    Roy Smith; Fiscal Year: 2007
    ..We hypothesize that clinical use of ghrelin mimetics will translate to improved cognitive function in the elderly. [unreadable] [unreadable] [unreadable]..
  55. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2007
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. [unreadable] [unreadable]..
  56. LARG, A Novel Leukemia Associated Guanine Exhange Factor
    Guido Marcucci; Fiscal Year: 2006
    ..i.). ..
  57. Matrix Metalloproteinases, Exercise, and Muscle Damage
    David Allen; Fiscal Year: 2008
    ..Finally, subjects will be genotyped for known functional MMP promoter polymorphisms, which will be correlated with MMP levels and muscle damage and soreness following eccentric exercise. ..
  58. CONTROL OF ADIPOGENESIS AND ENERGY METABOLISM
    Stephen Farmer; Fiscal Year: 2007
    ..4. To define the function of Ero1-Lalpha and associated proteins in regulating adiponectin secretion ..
  59. Somatomedin: Autologous Regulator of Bone Formation
    Ernesto Canalis; Fiscal Year: 2008
    ..These investigations should clarify the role of CHOP in bone cell differentiation and function. [unreadable] [unreadable]..
  60. 15th International RUNX Workshop
    LUCIO CASTILLA; Fiscal Year: 2008
    ..The 15th International RUNX Workshop is intended to maintain this tradition, thereby serving to enhance future efforts in cancer research and treatment. [unreadable] [unreadable] [unreadable]..
  61. INTERACTING FACTORS IN HEMATOPOIESIS AND LEUKEMIA
    Daniel Tenen; Fiscal Year: 2007
    ....
  62. Convergence of Nuclear Receptor and Forkhead Signaling
    Paul Dowell; Fiscal Year: 2004
    ..The excellent research environment at Johns Hopkins coupled with an extensive collaborative team of experts in relevant fields of study is ideal for successful completion of the proposed work. ..