nucleoproteins

Summary

Summary: Proteins conjugated with nucleic acids.

Top Publications

  1. ncbi The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication
    Agustín Portela
    División de Productos Biológicos y Biotecnología, Agencia Española del Medicamento, Majadahonda 28220, Madrid, Spain
    J Gen Virol 83:723-34. 2002
  2. pmc Differential inhibition of type I interferon induction by arenavirus nucleoproteins
    Luis Martinez-Sobrido
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 81:12696-703. 2007
  3. ncbi The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA
    Qiaozhen Ye
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, MS140, Houston, Texas 77005, USA
    Nature 444:1078-82. 2006
  4. ncbi Protection against multiple influenza A subtypes by vaccination with highly conserved nucleoprotein
    Suzanne L Epstein
    Laboratory of Immunology and Developmental Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, Bethesda, MD 20852, USA
    Vaccine 23:5404-10. 2005
  5. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
  6. pmc Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
    Kathryn M Hastie
    Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:2396-401. 2011
  7. pmc Cap binding and immune evasion revealed by Lassa nucleoprotein structure
    Xiaoxuan Qi
    Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Nature 468:779-83. 2010
  8. pmc Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis
    F Momose
    Laboratory of Molecular Medical Engineering, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan
    J Virol 75:1899-908. 2001
  9. pmc Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus
    Shenghua Zhou
    Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Virol 84:9452-62. 2010
  10. ncbi Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients
    S Baize
    Centre International de Recherches Medicales de Franceville, Gabon
    Nat Med 5:423-6. 1999

Detail Information

Publications340 found, 100 shown here

  1. ncbi The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication
    Agustín Portela
    División de Productos Biológicos y Biotecnología, Agencia Española del Medicamento, Majadahonda 28220, Madrid, Spain
    J Gen Virol 83:723-34. 2002
    ..The evidence for the existence of each of these activities and their possible roles in transcription, replication and intracellular trafficking of the virus genome is considered...
  2. pmc Differential inhibition of type I interferon induction by arenavirus nucleoproteins
    Luis Martinez-Sobrido
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 81:12696-703. 2007
    ..Consistent with this observation, TCRV-infected cells also failed to inhibit interferon production...
  3. ncbi The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA
    Qiaozhen Ye
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, MS140, Houston, Texas 77005, USA
    Nature 444:1078-82. 2006
    ..Our results identify the tail loop binding pocket as a potential target for antiviral development...
  4. ncbi Protection against multiple influenza A subtypes by vaccination with highly conserved nucleoprotein
    Suzanne L Epstein
    Laboratory of Immunology and Developmental Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, Bethesda, MD 20852, USA
    Vaccine 23:5404-10. 2005
    ..Thus, gene-based vaccination with NP may contribute to protective immunity against diverse influenza viruses through its ability to stimulate cellular immunity...
  5. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
    ..Consistent with this, NP bound CRM1 in vitro. Overall, these data raise the possibility that RNP export is mediated by a direct interaction between NP and the cellular CRM1 export pathway...
  6. pmc Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
    Kathryn M Hastie
    Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:2396-401. 2011
    ..Thus, the nucleoprotein is a viral exonuclease with anti-immune activity, and this work provides a unique opportunity to combat arenaviral infections...
  7. pmc Cap binding and immune evasion revealed by Lassa nucleoprotein structure
    Xiaoxuan Qi
    Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Nature 468:779-83. 2010
    ..These findings provide great potential for vaccine and drug development...
  8. pmc Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis
    F Momose
    Laboratory of Molecular Medical Engineering, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan
    J Virol 75:1899-908. 2001
    ..Together, these observations suggest that RAF-2p48 facilitates NP-RNA interaction, thus leading to enhanced influenza virus RNA synthesis...
  9. pmc Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus
    Shenghua Zhou
    Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Virol 84:9452-62. 2010
    ..These virus-host interactions may play important roles in the pathogeneses of LCMV and other human arenavirus diseases...
  10. ncbi Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients
    S Baize
    Centre International de Recherches Medicales de Franceville, Gabon
    Nat Med 5:423-6. 1999
    ..Thus, events very early in Ebola virus infection determine the control of viral replication and recovery or catastrophic illness and death...
  11. ncbi Characterization of the Lassa virus matrix protein Z: electron microscopic study of virus-like particles and interaction with the nucleoprotein (NP)
    Robert Eichler
    Institut für Virologie der Philipps Universität Marburg, Robert Koch Strasse 17, D 35037 Marburg, Germany
    Virus Res 100:249-55. 2004
    ..We assume that Z recruits NP to cellular membranes where virus assembly takes place. We conclude from our data that Lassa virus Z protein plays an essential role in Lassa virus maturation...
  12. pmc A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: implications for filovirus budding
    R N Harty
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104 6049, USA
    Proc Natl Acad Sci U S A 97:13871-6. 2000
    ..Last, these studies imply that VP40 likely plays an important role in filovirus budding, and that budding of retroviruses, rhabdoviruses, and filoviruses may proceed via analogous mechanisms...
  13. ncbi Importin alpha nuclear localization signal binding sites for STAT1, STAT2, and influenza A virus nucleoprotein
    Krister Melen
    Laboratory of Infectious Disease Immunology, Department of Microbiology, National Public Health Institute, FIN 00300 Helsinki, Finland
    J Biol Chem 278:28193-200. 2003
    ....
  14. ncbi Vesicular release of ebola virus matrix protein VP40
    J Timmins
    EMBL, 6 rue Jules Horowitz, 38042 Grenoble, France
    Virology 283:1-6. 2001
    ..Together, these results provide strong evidence that Ebola virus VP40 is sufficient for virus assembly and budding from the plasma membrane...
  15. pmc Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication
    Makoto Ozawa
    Division of Virology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639, Japan
    J Virol 81:30-41. 2007
    ..A virus with nonfunctional NLS2 could not be generated. Thus, the bipartite NLS, but not the unconventional NLS, of NP is essential for influenza A virus replication...
  16. pmc Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP
    Takeshi Noda
    Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Virol 76:4855-65. 2002
    ..These results demonstrated the central role of VP40 in formation of the filamentous structure of Ebola virions and may suggest an interaction between VP40 and GP in morphogenesis...
  17. ncbi HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase
    P Gallay
    Infectious Disease Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Cell 83:569-76. 1995
    ..It is blocked by phosphotyrosine, indicating that IN recognizes the phosphorylated C-terminal residue of MA. These results explain how the karyophilic potential of MA is conferred to the HIV nucleoprotein complex...
  18. ncbi Mammalian mitochondrial nucleoids: organizing an independently minded genome
    Ian J Holt
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 OXY, UK
    Mitochondrion 7:311-21. 2007
    ....
  19. pmc Cryo-electron tomography of Marburg virus particles and their morphogenesis within infected cells
    Tanmay A M Bharat
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 9:e1001196. 2011
    ....
  20. ncbi Molecular epidemiology of rabies in Indonesia
    Heru Susetya
    Department of Veterinary Medicine, Faculty of Applied Biological Sciences, The United School of Veterinary Sciences, Gifu University, 1 1 Yanagido, Gifu 501 1193, Japan
    Virus Res 135:144-9. 2008
    ..Results of phylogenetic analysis and historical background suggest that rabies viruses in China might have been transferred to Indonesia and spread to each island due to human activities...
  21. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
    ..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
  22. ncbi Structural insights into the retroviral DNA integration apparatus
    Peter Cherepanov
    Division of Infectious Diseases, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Curr Opin Struct Biol 21:249-56. 2011
    ....
  23. ncbi Small interfering RNAs targeting the rabies virus nucleoprotein gene
    Yu Jiao Yang
    Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China
    Virus Res 169:169-74. 2012
    ..These siRNAs have the potential to be developed into new and effective prophylactic anti-RABV drugs...
  24. ncbi Nucleoproteins and nucleocapsids of negative-strand RNA viruses
    Rob W H Ruigrok
    Unit of Virus Host Cell Interactions, UJF EMBL CNRS, UMI 3265, 6 rue Jules Horowitz, BP 181, 38042 Grenoble Cedex 9, France
    Curr Opin Microbiol 14:504-10. 2011
    ..The mechanism by which this specific association occurs, for both the segmented (s) and non-segmented (ns) viruses, has recently become clearer due to our expanding knowledge of N protein and nucleocapsid structures...
  25. ncbi Identification of influenza A nucleoprotein as an antiviral target
    Richard Y Kao
    Department of Microbiology, The University of Hong Kong, Hong Kong
    Nat Biotechnol 28:600-5. 2010
    ..Our results demonstrate that viral NP is a valid target for the development of small-molecule therapies...
  26. pmc Nuclear import of influenza A viral ribonucleoprotein complexes is mediated by two nuclear localization sequences on viral nucleoprotein
    Winco W H Wu
    Department of Zoology, University of British Columbia, Vancouver, BC, Canada
    Virol J 4:49. 2007
    ..These NP NLSs have been shown to mediate the nuclear import of recombinant NP molecules. However, it remains to be determined which NLS mediates the nuclear import of influenza vRNP complexes...
  27. ncbi A functional link between the actin cytoskeleton and lipid rafts during budding of filamentous influenza virions
    Martha Simpson-Holley
    Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
    Virology 301:212-25. 2002
    ..We propose that the actin cytoskeleton is required to maintain the correct organisation of lipid rafts for incorporation into budding viral filaments...
  28. pmc Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation
    Xin Zhang
    Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA
    J Virol 82:674-82. 2008
    ..These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA...
  29. pmc Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design
    Andy Ka Leung Ng
    Department of Biochemistry and Center for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
    FASEB J 22:3638-47. 2008
    ..Our study of H5N1 NP provides insight into the oligomerization interface and the RNA-binding groove, which are attractive drug targets, and it identifies the epitopes that might be used for universal vaccine development...
  30. ncbi An unconventional NLS is critical for the nuclear import of the influenza A virus nucleoprotein and ribonucleoprotein
    Jerome F Cros
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Traffic 6:205-13. 2005
    ..Finally, viral replication could be specifically inhibited by a membrane-permeable peptide containing the unconventional NLS, confirming the crucial role of this signal during the replicative cycle of the virus...
  31. ncbi Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Mol Biol 326:493-502. 2003
    ..These results suggest that hNedd4 and Tsg101 may play complimentary roles at a late stage of the assembly process, by recruiting cellular factors of two independent pathways to the site of budding at the plasma membrane...
  32. ncbi Protection against multiple influenza A virus subtypes by intranasal administration of recombinant nucleoprotein
    Lina Guo
    Shanghai Institute of Biological Products, China
    Arch Virol 155:1765-75. 2010
    ..The results indicate that such a vaccine administered intranasally can induce mucosal and cell-mediated immunity, thus having the potential to control epidemics caused by new emerging influenza viruses...
  33. pmc Influenza A virus strains differ in sensitivity to the antiviral action of Mx-GTPase
    Jan Dittmann
    Department of Virology, University of Freiburg, Hermann Herder Strasse 11, 79107 Freiburg, Germany
    J Virol 82:3624-31. 2008
    ....
  34. ncbi The matrix protein VP40 from Ebola virus octamerizes into pore-like structures with specific RNA binding properties
    F Xavier Gomis-Ruth
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042, Grenoble, France
    Structure 11:423-33. 2003
    ..SDS-resistant octameric VP40 is also found in Ebola virus-infected cells, which suggests that VP40 has an additional function in the life cycle of the virus besides promoting virus assembly and budding off the plasma membrane...
  35. pmc The accessory subunit of mitochondrial DNA polymerase gamma determines the DNA content of mitochondrial nucleoids in human cultured cells
    M Di Re
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, UK
    Nucleic Acids Res 37:5701-13. 2009
    ..These findings support the view that the mitochondrial D-loop acts as a protein recruitment centre, and suggest POLGbeta is a key factor in the organization of mitochondrial DNA in multigenomic nucleoprotein complexes...
  36. pmc Efficient budding of the tacaribe virus matrix protein z requires the nucleoprotein
    Allison Groseth
    Institut fur Virologie, Philipps Universitat Marburg, Hans Meerwein Str 2, Marburg 35043, Germany
    J Virol 84:3603-11. 2010
    ....
  37. ncbi Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription
    T Hoenen
    Institute for Virology, Philipps University Marburg, Marburg, Germany
    Virology 403:56-66. 2010
    ..Further, we show for the first time that VP40 is also involved in regulating these processes. These functions are conserved among EBOV species and, in the case of VP40, independent of its budding or RNA-binding functions...
  38. pmc Enzyme-linked immunosorbent assays for detection of antibodies to Ebola and Marburg viruses using recombinant nucleoproteins
    M Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Clin Microbiol 39:1-7. 2001
    ..The results indicate that ELISA systems prepared with the recombinant NPs of EBO and MBG are valuable tools for the diagnosis of EBO and MBG infections and for seroepidemiological field studies...
  39. ncbi Lipid raft-dependent targeting of the influenza A virus nucleoprotein to the apical plasma membrane
    Marlene Carrasco
    Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Traffic 5:979-92. 2004
    ..We conclude that NP is independently targeted to the apical plasma membrane through a mechanism involving lipid rafts and propose that this helps determine the polarity of influenza virus budding...
  40. pmc Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import
    P Gallay
    Infectious Disease Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Virol 70:1027-32. 1996
    ..In addition, our data suggest that Vpr governs HIV-1 nuclear import through a distinct pathway...
  41. pmc Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L
    Renata Z Jurkowska
    Biochemistry Lab, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany
    Nucleic Acids Res 36:6656-63. 2008
    ..Scanning force microscopy demonstrates filament formation rather than binding of single tetramers and shows that protein-DNA filament formation leads to a 1.5-fold shortening of the DNA length...
  42. pmc Structural basis for the dsRNA specificity of the Lassa virus NP exonuclease
    Kathryn M Hastie
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 7:e44211. 2012
    ....
  43. pmc Identification of amino acid residues critical for the anti-interferon activity of the nucleoprotein of the prototypic arenavirus lymphocytic choriomeningitis virus
    Luis Martinez-Sobrido
    Department of Immunology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York 10029, USA
    J Virol 83:11330-40. 2009
    ..In addition, A549 cells infected with rLCMV/NP*(D382A), but not with WT LCMV, produced IFN and failed to rescue replication of the IFN-sensitive Newcastle disease virus...
  44. pmc A universal influenza A vaccine based on adenovirus expressing matrix-2 ectodomain and nucleoprotein protects mice from lethal challenge
    Dongming Zhou
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Ther 18:2182-9. 2010
    ..Most importantly, vaccinated mice are protected against morbidity and mortality following challenge with high doses of different influenza virus strains. Protection requires both antibodies to M2e and cellular immune responses to NP...
  45. pmc Oligomerization of Ebola virus VP40 is essential for particle morphogenesis and regulation of viral transcription
    T Hoenen
    Institut fur Virologie, Hans Meerwein Str 2, 35043 Marburg, Germany
    J Virol 84:7053-63. 2010
    ....
  46. pmc Cross-species analysis of the replication complex of Old World arenaviruses reveals two nucleoprotein sites involved in L protein function
    Romy Kerber
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    J Virol 85:12518-28. 2011
    ..Residue 53 and the C-terminal domain of NP are important for function of L protein during genome replication and transcription...
  47. ncbi Effective small interfering RNAs targeting matrix and nucleocapsid protein gene inhibit influenza A virus replication in cells and mice
    Hongbo Zhou
    National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China
    Antiviral Res 76:186-93. 2007
    ..The results provided a basis for further development of siRNA for prophylaxis and therapy of influenza virus infection in humans and animals...
  48. ncbi [Comparative study of the morphology and antigenic properties of recombinant analogs of a Marburg virus nucleoprotein]
    A V Kachko
    Mol Biol (Mosk) 35:492-9. 2001
    ..coli and of a single type in cell line 293. ELISA and immunoblotting with polyclonal and monoclonal antibodies revealed common antigenic determinants in recombinant NP and natural MV NP...
  49. pmc Characterization of the Ebola virus nucleoprotein-RNA complex
    Takeshi Noda
    International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Gen Virol 91:1478-83. 2010
    ..These findings enhance our knowledge of Ebola virus assembly and understanding of the Ebola virus life cycle...
  50. pmc New measles virus genotype associated with outbreak, China
    Yan Zhang
    National Institute for Viral Disease Control and Prevention, Beijing, People s Republic of China
    Emerg Infect Dis 16:943-7. 2010
    ..The new genotype designation will allow for better description of measles transmission patterns, especially in the Southeast Asian and Western Pacific regions...
  51. pmc Comparative efficacy of hemagglutinin, nucleoprotein, and matrix 2 protein gene-based vaccination against H5N1 influenza in mouse and ferret
    Srinivas S Rao
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 5:e9812. 2010
    ..These immunogens may require combinatorial vaccination with HA, which confers protection even against very high doses of lethal viral challenge...
  52. ncbi Structural disorder within paramyxovirus nucleoproteins and phosphoproteins
    Johnny Habchi
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS et Universités d Aix Marseille I et II, Marseille, France
    Mol Biosyst 8:69-81. 2012
    ....
  53. pmc Direct observation of twisting steps during Rad51 polymerization on DNA
    Hideyuki Arata
    Institut Curie, Centre National de la Recherche Scientifique, Universite Pierre et Marie Curie, Unité Mixte de Recherche 168, 75231 Paris, France
    Proc Natl Acad Sci U S A 106:19239-44. 2009
    ....
  54. pmc Partial protection against H5N1 influenza in mice with a single dose of a chimpanzee adenovirus vector expressing nucleoprotein
    Soumitra Roy
    Gene Therapy Program, Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, PA 19104 4268, USA
    Vaccine 25:6845-51. 2007
    ....
  55. ncbi Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses
    Ami Patel
    Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
    Vaccine 27:3083-9. 2009
    ..This study emphasizes the need to evaluate the relationship between both arms of the adaptive immune responses in regards to protective efficacy against influenza virus...
  56. pmc Recent developments in single-molecule DNA mechanics
    Zev Bryant
    Department of Bioengineering, Stanford University, Stanford, CA 94305, USA
    Curr Opin Struct Biol 22:304-12. 2012
    ..The new measurements have also been exploited in studies that hint at a developing synergistic relationship between single-molecule manipulation and structural DNA nanotechnology...
  57. ncbi Phylogenetic comparison of the genus Lyssavirus using distal coding sequences of the glycoprotein and nucleoprotein genes
    N Johnson
    Rabies Research and Diagnostic Group, Veterinary Laboratories Agency, Weybridge, UK
    Arch Virol 147:2111-23. 2002
    ..Comparison of the phylogenetic analysis for this partial region of the glycoprotein suggest that it gives comparable results to studies that have used larger regions of the Lyssavirus genome...
  58. pmc Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPs
    Nancy J Sullivan
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Med 3:e177. 2006
    ..GP can exert cytopathic effects on transfected cells in vitro, and multiple GP forms have been identified in nature, raising the question of which would be optimal for a human vaccine...
  59. ncbi Human asymptomatic Ebola infection and strong inflammatory response
    E M Leroy
    Centre International de Recherches Medicales de Franceville, Gabon
    Lancet 355:2210-5. 2000
    ..We aimed to determine whether these individuals were indeed infected with Ebola virus, and how they maintained asymptomatic status...
  60. ncbi Development of an immunofluorescence method for the detection of antibodies to Ebola virus subtype Reston by the use of recombinant nucleoprotein-expressing HeLa cells
    Tetsuro Ikegami
    Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan
    Microbiol Immunol 46:633-8. 2002
    ..These results suggest that this new IFA is useful for seroepidemiological studies of EBO-R infection among monkeys...
  61. ncbi Stabilization of chromatin structure by PRC1, a Polycomb complex
    Z Shao
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Cell 98:37-46. 1999
    ..Several different types of repressive complexes, including deacetylases, interact with histone tails. In contrast, PRC1 was active on nucleosomal arrays formed with tailless histones...
  62. pmc Ebola virus matrix protein VP40 uses the COPII transport system for its intracellular transport
    Seiya Yamayoshi
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639, Japan
    Cell Host Microbe 3:168-77. 2008
    ..These findings identify a conserved intersection between a host pathway and filovirus replication, an intersection that can be targeted in the development of new antiviral drugs...
  63. pmc Structural comparisons of the nucleoprotein from three negative strand RNA virus families
    Ming Luo
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Virol J 4:72. 2007
    ..Two helices connecting the two domains characterize the center of the cavity. The nucleoproteins contain at least 5 conserved helices in the N-terminal domain and 3 conserved helices in the C-terminal domain...
  64. pmc Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology
    Reed F Johnson
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104, USA
    Virol J 3:31. 2006
    ..Overall, these findings indicate that major changes in morphology of VP40 VLPs were likely not responsible for enhanced budding of VP40 VLPs in the presence of GP, NP and/or VP35...
  65. pmc In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding
    Rekha G Panchal
    Developmental Therapeutics Program, Target Structure Based Drug Discovery Group, Science Applications International Corporation, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 100:15936-41. 2003
    ..We also report the successful application of the biarsenic fluorophore, FlAsH, combined with a tetracysteine tag for imaging of Ebola VP40 in live cells...
  66. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
    ..This distinct mechanism of regulation for filovirus assembly suggests new approaches for viral therapies and vaccines for Ebola and related viruses...
  67. ncbi Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography
    Takeshi Noda
    Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Vet Med Sci 67:325-8. 2005
    ..Reconstruction of these tubular NC-like structures of Ebola virus showed them to be composed of left-handed helices spaced at short intervals, which is structurally consistent with other non-segmented negative-strand RNA viruses...
  68. pmc Budding of PPxY-containing rhabdoviruses is not dependent on host proteins TGS101 and VPS4A
    Takashi Irie
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 78:2657-65. 2004
    ....
  69. pmc Memory T cells established by seasonal human influenza A infection cross-react with avian influenza A (H5N1) in healthy individuals
    Laurel Yong Hwa Lee
    MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Clin Invest 118:3478-90. 2008
    ..Thus, vaccine formulas inducing heterosubtypic T cell-mediated immunity may confer broad protection against avian and human influenza A viruses...
  70. pmc The Ebola virus VP35 protein functions as a type I IFN antagonist
    C F Basler
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 97:12289-94. 2000
    ..The Ebola virus VP35 therefore is likely to inhibit induction of type I IFN in Ebola virus-infected cells and may be an important determinant of Ebola virus virulence in vivo...
  71. pmc Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome
    Kadir Turan
    University of Marmara, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Haydarpasa, Kadikoy, Istanbul 34668, Turkey
    Nucleic Acids Res 32:643-52. 2004
    ..This indicates that the nuclear MxA suppresses the influenza virus transcription by interacting with not only PB2 but also NP...
  72. ncbi Phylogeny of New World arenaviruses based on the complete coding sequences of the small genomic segment identified an evolutionary lineage produced by intrasegmental recombination
    Remi N Charrel
    Unité des Virus Emergents EA 3292, IFR 48, Universite de la Mediterranee, Faculte de Medecine, 27 Blvd Jean Moulin, 13005, Marseille, France
    Biochem Biophys Res Commun 296:1118-24. 2002
    ..The recombinant nature of the S-RNA of NA-AVs distinguishes them from the SA-AVs, and thus, indicates that the NA-AVs represent a fourth phylogenetic lineage in the Tacaribe serocomplex...
  73. ncbi Virus-like particles exhibit potential as a pan-filovirus vaccine for both Ebola and Marburg viral infections
    Dana L Swenson
    United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702 5011, USA
    Vaccine 23:3033-42. 2005
    ..This work provides a critical foundation towards the development of a pan-filovirus vaccine that is safe and effective for use in primates and humans...
  74. pmc Human immunodeficiency virus type 1 Vif protein is packaged into the nucleoprotein complex through an interaction with viral genomic RNA
    M A Khan
    Laboratory of Molecular Microbiology, Viral Biochemistry Section, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892-0460, USA
    J Virol 75:7252-65. 2001
    ..Our data suggest that the specific association of Vif with the viral nucleoprotein complex might be functionally significant and could be a critical requirement for infectivity of viruses produced from restrictive host cells...
  75. pmc The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded
    Jean Marie Bourhis
    Architecture et Fonction des Macromolécules Biologiques AFMB, UMR 6098 CNRS at Universités Aix Marseille I et II, ESIL, Campus de Luminy, 13288 Marseille Cedex 09, France
    Protein Sci 14:1975-92. 2005
    ..This work provides evidence that intrinsically disordered domains can establish complex interactions with their partners, and can contact them through multiple sites that do not all necessarily gain regular secondary structure...
  76. ncbi Age-associated decrease in virus-specific CD8+ T lymphocytes during primary influenza infection
    John Leander Z Po
    Department of Microbiology and Immunology, MCP Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Mech Ageing Dev 123:1167-81. 2002
    ..These data suggest that the age-related impairment of CD8+ lymphocyte activity during a primary influenza A infection is due to a defect in the expansion, rather than in effector activity, of influenza-specific CD8+ T cells...
  77. pmc A virus-specific CD8+ T cell immunodominance hierarchy determined by antigen dose and precursor frequencies
    Nicole L La Gruta
    Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia
    Proc Natl Acad Sci U S A 103:994-9. 2006
    ..Thus, this immunodominance hierarchy is a direct function of antigen dose and T cell numbers...
  78. ncbi The transpososome: control of transposition at the level of catalysis
    E Gueguen
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 route de Narbonne, 31062 Toulouse Cedex, France
    Trends Microbiol 13:543-9. 2005
    ..This has been underpinned by recent results obtained particularly with Tn5, Tn10 and bacteriophage Mu...
  79. ncbi Development of real-time reverse transcriptase polymerase chain reaction methods for human rabies diagnosis
    Susan A Nadin-Davis
    Ottawa Laboratory, Canadian Food Inspection Agency, Ontario, Canada
    J Med Virol 81:1484-97. 2009
    ..However in this case it would appear that reduction of the viral load in the patient's saliva over time did not appear to correlate well with clearance of viral components from the brain...
  80. ncbi Oligomerization and polymerization of the filovirus matrix protein VP40
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    Virology 312:359-68. 2003
    ..Interestingly, Marburg virus VP40 rings reveal a high tendency to polymerize into rods composed of stacked rings. These results may suggest distinct roles for different oligomeric forms of VP40 in the filovirus life cycle...
  81. ncbi Crystal structure of the measles virus phosphoprotein domain responsible for the induced folding of the C-terminal domain of the nucleoprotein
    Kenth Johansson
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS et Université Aix Marseille, 13288 Marseille 09, France
    J Biol Chem 278:44567-73. 2003
    ..These results, beyond presenting the first measles virus protein structure, shed light both on the function of the phosphoprotein at the molecular level and on the process of induced folding...
  82. pmc Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Clin Microbiol 40:1587-91. 2002
    ..These results suggest that the IgG ELISA system developed with the recombinant CCHFV NP is a valuable tool for diagnosis and epidemiological investigations of CCHFV infections...
  83. pmc The distribution of polycomb-group proteins during cell division and development in Drosophila embryos: impact on models for silencing
    P Buchenau
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    J Cell Biol 141:469-81. 1998
    ..These observations have important implications for models of the mechanism and maintenance of PcG- mediated gene repression...
  84. pmc Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene
    Hirohito Ogawa
    Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia
    J Virol Methods 171:310-3. 2011
    ..This assay will be a useful method for diagnostics and epidemiological studies of filovirus infections...
  85. pmc Packaging of actin into Ebola virus VLPs
    Ziying Han
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Virol J 2:92. 2005
    ..These data suggest that VP40 may interact with cellular actin, and that actin may play a role in assembly and/or budding of Ebola VLPs...
  86. pmc A role for the C terminus of Mopeia virus nucleoprotein in its incorporation into Z protein-induced virus-like particles
    Olena Shtanko
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 575 Science Drive, Madison, WI 53711, USA
    J Virol 84:5415-22. 2010
    ..By employing a series of NP deletion constructs and testing their VLP incorporation, we further demonstrated an important role for the C-terminal half of NP in its incorporation into VLPs...
  87. ncbi Identification and characterization of polyhomeotic PREs and TREs
    Sebastien Bloyer
    Institut de Genetique Humaine, C N R S U P R 1142, 141 rue de la Cardonille, 34396 5, Montpellier Cedex, France
    Dev Biol 261:426-42. 2003
    ..Our data suggest that ph PRE/TREs are cis-acting DNA elements that modulate rather than silence Pc-G- and trx-G-mediated regulation, enlarging the role of these two groups of genes in transcriptional regulation...
  88. pmc Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus
    T Ikegami
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    Epidemiol Infect 130:533-9. 2003
    We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z)...
  89. ncbi Visualizing the viral burden: phenotypic and functional alterations of T cells and APCs during persistent infection
    Dirk Homann
    Department of Neuropharmacology, Division of Virology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 172:6239-50. 2004
    ....
  90. ncbi Analysis of Ebola virus and VLP release using an immunocapture assay
    George Kallstrom
    Division of Virology, U S Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
    J Virol Methods 127:1-9. 2005
    ..Furthermore, this assay can be used in surrogate models in non-biocontainment environment, facilitating both basic research on the mechanism of EBOV assembly and budding as well as drug-discovery research...
  91. pmc Role of the virus nucleoprotein in the regulation of lymphocytic choriomeningitis virus transcription and RNA replication
    Daniel D Pinschewer
    Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 77:3882-7. 2003
    ..Our data are consistent with a central role for NP in transcription and replication of the LCMV genome, but they do not support the participation of NP levels in balancing the two processes...
  92. ncbi Developmental dynamics of a polyhomeotic-EGFP fusion in vivo
    S Netter
    , , CNRS, UPRES-A 8080, , Orsay, France
    DNA Cell Biol 20:483-92. 2001
    ..The increasing number of dots observed during development may indicate that PH-EGFP is recruited at different stages on different target sites, a result that is in good agreement with functional data previously reported...
  93. pmc The nucleoprotein of lymphocytic choriomeningitis virus facilitates spread of persistent infection through stabilization of the keratin network
    Martina Labudova
    Institute of Virology, Bratislava, Slovak Republic
    J Virol 83:7842-9. 2009
    ....
  94. ncbi The 12 A structure of trypsin-treated measles virus N-RNA
    Guy Schoehn
    EMBL Grenoble Outstation, 6 rue Jules Horowitz, B P 181, 38042 Grenoble 9, France
    J Mol Biol 339:301-12. 2004
    ..The shapes of the monomers of the nucleoproteins of influenza virus, rabies virus (both determined before) and that of measles virus (determined here) are all ..
  95. pmc Transgenic mice expressing the nucleoprotein of Borna disease virus in either neurons or astrocytes: decreased susceptibility to homotypic infection and disease
    Mathias Rauer
    Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, D 79104 Freiburg, Germany
    J Virol 78:3621-32. 2004
    ..Thus, while resistance to BDV infection in N-expressing neurons appeared to result from untimely expression of a viral nucleocapsid component, the resistance to BDV-induced neuropathology probably resulted from immunological tolerance...
  96. ncbi Newcastle disease outbreaks in western China were caused by the genotypes VIIa and VIII
    R Liang
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001, PR China
    Vet Microbiol 87:193-203. 2002
    ....
  97. pmc Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies
    Violaine Lantez
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 Centre National de la Recherche Scientifique, Université de la Méditerranée and Université de Provence, Marseille, France
    PLoS Negl Trop Dis 5:e936. 2011
    b>Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies...
  98. ncbi Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy
    Marlène Martinho
    CNRS, Aix Marseille Universite, IMM FR 3479, BIP UMR 7281, 13402 Marseille, France
    J Biomol Struct Dyn 31:453-71. 2013
    ..Altogether these results provide insights into the molecular mechanisms of the Henipavirus NTAIL-PXD binding reaction...
  99. pmc Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles
    Jillian M Licata
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104 6049, USA
    J Virol 78:7344-51. 2004
    ..the C-terminal 50 amino acids of NP may be important for interacting with and enhancing release of VP40 VLPs. These findings provide a more complete understanding of the role of VP40 and additional Ebola virus proteins during budding...
  100. pmc Arenavirus nucleoproteins prevent activation of nuclear factor kappa B
    W W Shanaka I Rodrigo
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 86:8185-97. 2012
    ..Altogether, our findings demonstrate that arenavirus infection inhibits NF-κB-dependent innate immune and inflammatory responses, possibly playing a key role in the pathogenesis and virulence of arenavirus...
  101. ncbi A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins
    T Wolff
    Institut fur Virologie, Philipps Universitat Marburg, Robert Koch str 17, 35037 Marburg, Germany
    J Gen Virol 81:939-47. 2000
    ..These findings are discussed with respect to possible roles for the p10 protein in viral RNA synthesis or ribonucleoprotein transport...

Research Grants72

  1. Histone Chaperones in Angiogenesis
    Soumen Paul; Fiscal Year: 2012
    ....
  2. Defining the telomerase holoenzyme in progenitor cells with aging
    Steven E Artandi; Fiscal Year: 2013
    ..An improved understanding of telomerase is crucial for designing therapies that will delay or reverse certain aspects of aging. ..
  3. Identification and characterization of inhibitors for hantavirus replication
    MOHAMMAD AYOUB MIR; Fiscal Year: 2013
    ....
  4. The influenza A virus nucleoprotein: structure and function
    Yizhi Jane Tao; Fiscal Year: 2012
    ..The aim of this grant is to elucidate the molecular mechanism and biological significance of several key activities of the influenza A virus nucleoprotein and to discover how such activities can be exploited for antiviral development. ..
  5. STRUCTURAL STUDIES OF NUCLEOPROTEIN COMPLEXES
    Edward H Egelman; Fiscal Year: 2012
    ..And other complexes are involved in the assembly of viruses, including one that causes leukemia. ..
  6. LOCALIZED REVISION OF EPIGENETIC LANDSCAPES INDUCED BY DNA DOUBLE-STRAND BREAKS
    Barry P Sleckman; Fiscal Year: 2012
    ..function, in part, by modulating chromatin structure to render associated genes more or less accessible to nucleoproteins that mediate these biological processes...
  7. Mechanisms of non-neutralizing antibody-mediated protection from influenza virus
    DENISE KAMINSKI; Fiscal Year: 2009
    ....
  8. Strain Specific Detection of Influenza at the Point-of-Care
    HYONGSOK TOM SOH; Fiscal Year: 2012
    ..1 ..
  9. Structural and Mechanistic Characterization of the Histone Chaperone FACT
    Duane David Winkler; Fiscal Year: 2012
    ..Insights from these proposed aims could aid in the development of specific FACT inhibitors that have the potential to increase chemotherapy effectiveness while decreasing aberrant cancer-related processes. ..
  10. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..To this end, this proposal aims to illuminate the structural properties of telomerase that underlie its unique cellular activity. ..
  11. Transcriptional Regulation of Hepatocyte Differentiation and Function
    Lisa A Cirillo; Fiscal Year: 2013
    ..Subversion of these gene regulatory events is a likely contributor to metabolic derangements and hepatic disease, making it vital that we uncover the key mechanisms and players. ..
  12. REGULATORY INTERACTIONS BETWEEN TUMOR VIRUSES AND CELLS
    Michael R Botchan; Fiscal Year: 2013
    ..The signaling events that control the action of replication proteins, will be provide insights into the networks that control cell cycle progression and should help in cancer therapy and diagnosis...
  13. Elucidating the role of TIN2L at the telomere and in dyskeratosis congenita
    Nya D Nelson; Fiscal Year: 2013
    ..This may ultimately have implications for sporadic cancer. ..
  14. HIV integrase/DNA complexes and concerted integration
    Duane P Grandgenett; Fiscal Year: 2010
    ..We will determine if LEDGF/p75 physically interacts with SC and test whether it influences host-site selection on chromatinized supercoiled target DNA that contains HIV-1 host-site consensus sequences. ..
  15. Chromatin and Coactivators in HSV-1 gene regulation
    STEVEN TRIEZENBERG; Fiscal Year: 2009
    ..Early stages in virus infection seem to depend on certain enzymes from the host cell. This research will validate the roles of those enzymes in virus infection, which may lead to new antiviral drug targets. ..
  16. Nucleoprotein Structures at Telomeres and Sites of DNA Damage
    Jack D Griffith; Fiscal Year: 2013
    ..These studies have very high impact since no other laboratory is applying this technology to telomere/repair work and many other laboratories depend on the input from these structural studies. . ..
  17. Interplay between Chromatin and Co-activator Complexes
    Michael F Carey; Fiscal Year: 2012
    ..Our proposal will use a model system to understand the biochemical details and mechanism of this process. ..
  18. Error Correction in DNA Synthesis: A Biochemical Study
    MYRON GOODMAN; Fiscal Year: 2013
    ..This study explores biochemical mechanisms that govern the ability of error-prone DNA polymerases to copy damaged DNA that would otherwise cause a cessation of chromosome replication resulting in cell death. ..
  19. Evaluation of LASV Protective Immunity in Mice Using Reassortant Virus ML29
    MARCO AURELIO GOICOCHEA; Fiscal Year: 2010
    ..The proposed mouse model will allow the evaluation of immunogenicity to a Lassa like-infection and the evaluation of Lassa fever vaccine candidates. ..
  20. Molecular Determinants that Govern Ku's Association with Telomeres
    SANDRA MELANIE LOUISE INDIVIGLIO; Fiscal Year: 2012
    ..Thus, the studies proposed may take us one step further to understanding the mechanism by which Ku suppresses aging, and may ultimately lead to insight into aging-related diseases. ..
  21. Nanomedicine Center for Nucleoprotein Machines
    Gang Bao; Fiscal Year: 2013
    ..abstract_text> ..
  22. Control of Telomere Heterochromatin by TERRA
    Paul M Lieberman; Fiscal Year: 2013
    ..The long-term goal of this proposal is to determine how telomeric heterochromatin is deregulated in cellular aging and cancer. ..
  23. Arenavirus Subversion of Dendritic Cells
    Elina I Zuniga; Fiscal Year: 2013
    ..The long-term impact of our studies will be to translate this new knowledge into novel therapeutic modalities in human infections with arenaviruses and may be other DC-tropic viruses. ..
  24. Roles of the RecQ Helicases BLM and RECQ5 in Genome Maintenance
    Patrick Sung; Fiscal Year: 2013
    ..In this renewal project, we will strive to delineate the multi-faceted role of BLM in HR repair and regulation, and to also define the mechanism underlying the novel role of RECQ5 as an anti-recombinase. ..
  25. GROWTH HORMONE SIGNALING TO THE NUCLEUS
    Jessica Schwartz; Fiscal Year: 2010
    ..With this information, we can identify new molecular targets to develop more effective therapies for conditions responsive to GH and to avoid GH-related complications in conditions such as diabetes and cancer. ..
  26. Anti-interferon Activity of the Arenavirus Nucleoprotein
    Luis Martinez-Sobrido; Fiscal Year: 2012
    ..This knowledge should fuel the development of better antiviral strategies to combat human pathogenic arenaviruses. ..
  27. Novel Mechanism of Immune Evasion by Arenaviral Nucleoproteins
    Hinh Ly; Fiscal Year: 2013
    ....
  28. Mechanisms of Transcription-Coupled DNA Supercoiling
    Fenfei Leng; Fiscal Year: 2012
    ..It also provides a foundation for further understanding DNA topology, which plays an important role in genome stability and certain human hereditary diseases, such as fragile X syndrome and Huntington's disease. ..
  29. Surveillance and maintenance of DNA ends
    KATSONURI SUGIMOTO; Fiscal Year: 2013
    ....
  30. The Roles of the Ku Heterodimer in Yeast Telomere Function
    ALISON ANN BERTUCH; Fiscal Year: 2011
    ....
  31. Telomere Structure and Function in Arabidopsis
    DOROTHY SHIPPEN; Fiscal Year: 2013
    ..In this tradition, we will exploit the genetic tractability of Arabidopsis and its extraordinary tolerance to telomere dysfunction to investigate a new telomere capping complex in multicellular organisms. ..
  32. Intracellular Defenses Against Foreign DNA: Insights From Poxvirus-Infected Cells
    Matthew S Wiebe; Fiscal Year: 2013
    ..At the conclusion of this work, the insights gained will further our understanding not only of poxviral- host interaction, but will be broadly significant to the understanding of immune responses to DNA in general. ..
  33. Agonists and Antagonists of Neuropsychiatric Lupus
    Keith B Elkon; Fiscal Year: 2012
    ..In our previously funded R21 proposal, we hypothesized that cell death coupled with the release of nucleoproteins resulted in the formation of immune complexes that stimulate Toll like receptors (TLRs) to produce high local ..
  34. Activation of Plp gene expression in oligodendrocytes
    Patricia A Wight; Fiscal Year: 2010
    ..Transgenic mice will also be generated to test whether the ASE and wmN1 enhancer work independently, or in combination with one another, to significantly increase the levels of Plp1 gene expression during development. ..
  35. GENE AMPLIFICATION BY A TRANSPOSITION APPARATUS
    Hiroshi Nakai; Fiscal Year: 2010
    ..This work will further define the cellular apparatus that maintains the stability and integrity of the genome, preventing genetic alterations associated with cellular dysfunction. ..
  36. The structure of telomerase RNA during catalysis
    DAUD COLE; Fiscal Year: 2012
    ..SHAPE technology will then be used to reveal the secondary structure of tTR during these processes. ..
  37. Cytology and function of ParA in E. coli
    JOSEPH A POGLIANO; Fiscal Year: 2010
    ..The findings from this analysis of a representative ParAB plasmid partitioning system should have wide implications for our understanding of plasmid and chromosome segregation. ..
  38. A Proteomic and Biochemical Study of HIV-1 Nucleoprotein Complexes
    MICHAEL A BELSHAN; Fiscal Year: 2013
    ..The goal of this proposal is to identify and characterize candidate cellular proteins associated with HIV-1 preintegration complexes for the development of new antiretroviral therapeutics. ..
  39. BIOCHEMICAL BASIS OF SOS-INDUCED MUTAGENESIS
    MYRON GOODMAN; Fiscal Year: 2013
    ..This new polymerase exchange model suggests the possibility that Pol V gains access to damaged DNA by "attacking" a stalled Pol III at a blocked replication fork. ..
  40. Telomere replication and maintenance of genome stability
    SANDY S CHANG; Fiscal Year: 2013
    ....
  41. Evolution of Telomere End Protection
    ROBERT ANTHONY HOM; Fiscal Year: 2013
    ..We will also elucidate how the recognition of telomeric DNA has changed over time from different species. This in vitro data will provide a novel connection of how end-protection has evolved. ..
  42. Developing Avirulent Rabies Virus Vaccines
    Zhen F Fu; Fiscal Year: 2010
    ..Such characterists will make these recombinant RV vaccines particularly suitable for post-exposure treatment. ..
  43. IMMUNOGLOBULIN AND T CELL RECEPTOR GENE ASSEMBLY
    David G Schatz; Fiscal Year: 2013
    ..abstract_text> ..
  44. Influenza nucleoprotein interactions and viral RNA synthesis
    LAURA LYNN NEWCOMB; Fiscal Year: 2013
    ....
  45. Regulating Influenza Polymerase Structure and Function by Phosphorylation
    Andrew Mehle; Fiscal Year: 2010
    ..These studies will provide insight into the regulation of the influenza replication machinery, how it controls transmission of influenza virus from birds to humans, and may ultimately identify new targets for therapeutic intervention. ..
  46. Eukaryotic Telomere Dynamics
    ARTHUR LUSTIG; Fiscal Year: 2009
    ..This study will provide insights into comparable processes in higher eukaryotes that have been liked to oncogenesis and senescence. . ..
  47. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2013
    ..The results from our research endeavors have direct relevance to understanding the role of homologous recombination in cancer suppression. ..
  48. Identification of Histone Modification Interaction Networks
    MARK R contact PARTHUN; Fiscal Year: 2010
    ..An understanding of how histone modifications function is important for human health as defects in the regulation of histone modifications and chromatin structure play critical roles in diseases such as cancer. ..
  49. Cap snatching mechanism of arenaviruses
    Hinh Ly; Fiscal Year: 2011
    ..abstract_text> ..
  50. SINGLE STRANDED DNA RECOGNITION IN TELOMERES
    Deborah S Wuttke; Fiscal Year: 2013
    ..This hypothesis-driven, highly unified research program will provide novel insights into the fundamental processes that maintain a central mechanism of chromosome stability. ..
  51. A comparative structural study of ATP-dependent chromatin remodeling complexes
    Andres Leschziner; Fiscal Year: 2013
    ..We aim to understand, at the molecular level, how these complexes interact with and remodel nucleosomes. Our research could lead to new insights in the fields of epigenetics and cancer. ..
  52. Functions of Rad51 Paralogs in Recombinational DNA Repair
    STEPHEN CHARLES KOWALCZYKOWSKI; Fiscal Year: 2013
    ..These measurements will use direct imaging by single-molecule fluorescence microscopy of hRAD51 filament dynamics on individual molecules of DNA. ..
  53. MOLECULAR ANALYSIS OF SITE SPECIFIC DNA RECOMBINATION
    Reid C Johnson; Fiscal Year: 2013
    ..Fis effects on chromosome structure will be addressed by a combination of in vivo and in vitro approaches, including collaborative single-DNA molecule approaches as well as by bulk-phase in vitro and in vivo experiments. ..
  54. Regulating Influenza Polymerase Structure and Function by Phosphorylation
    Andrew Mehle; Fiscal Year: 2013
    ..abstract_text> ..
  55. Molecular determinants of host-cell interactions required for arenavirus replicat
    Jack H Nunberg; Fiscal Year: 2013
    ..We anticipate that essential virus-cell interactions can be targeted to generate highly specific antiviral effects that are resistant to genetic escape. ..
  56. Structure/Function of Transcription Complex Regulation
    Robert Landick; Fiscal Year: 2013
    ..It also will contribute to the development of an important new technology of DNA sequence-targeted unbiased proteomics with broad applications in biotechnology, human medicine, and both prokaryotic and eukaryotic molecular biology. ..
  57. Engineered virus-like nanoparticles targeting immunity needed for pan flu vaccine
    Allen G Harmsen; Fiscal Year: 2013
    ..The goal of this proposal is to integrate these two antigens (csHA and NP) with a highly engineer-able nano- platform to elicit protective immune responses in an effort aimed at the development of a universal influenza vaccine. ..
  58. CRYSTALLOGRAPHIC STUDIES ON RIBOSOMAL PARTICLES
    ADA E YONATH; Fiscal Year: 2010
    ..These studies have already illuminated principles of drug selectivity, and provided significant basic knowledge of possible pathways of drug resistance, hence paving the way to improve therapeutic properties. ..
  59. RTB-mediated delivery: Orchestrating antigen trafficking to enhance cell immunity
    Carole L Cramer; Fiscal Year: 2013
    ....
  60. Functional connections between histone variants and histone chaperones
    Karolin Luger; Fiscal Year: 2013
    ..We challenge current views of nucleosome conformation, and describe structural states that have enormous potential to impact genome organization and accessibility. ..
  61. Transposition of Tn7 and Hermes
    Nancy Craig; Fiscal Year: 2009
    ..We will also examine how the transposase-mediated breaks can lead to chromosomal rearrangements, i.e. genetic instability. ..
  62. Viral and Genetic Regulation of Abnormal OCL Activity in PD
    DEBORAH LYNN GALSON; Fiscal Year: 2012
    ..responsible for MVNP and p62p392L effects on OCL formation outlined above" will provide important insight into the pathogenesis of PD and are directly linked to the cellular and in vivo studies proposed in Project 2, ..
  63. Recombinant antigen assays for Lassa and other arenaviru
    Robert Garry; Fiscal Year: 2005
    ....
  64. Structural mechanism of DNA segregation by the pSK41 par system
    Maria Schumacher; Fiscal Year: 2010
    ..Thus, understanding the structural basis for DNA segregation by this par system will provide several points of potential therapeutic intervention against pSK41 harboring multidrug resistant S. aureus strains. ..
  65. Regulation of histone genes upon replication arrest and DNA damage
    HYERYUN PAIK; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  66. PHOSPHORYLATION AND TRANSPORT OF VIRAL NUCLEOPROTEINS
    Mario Stevenson; Fiscal Year: 2001
    ..Aim 2: Examine how cytoskeltal engagement influences the reverse transcription capacity of the reverse transcription complex and how this dependence is influenced by gag MA phosphorylation. ..
  67. SEQUENCING OF CLASS I MHC PEPTIDES BY MASS SPECTROMETRY
    Robert Cotter; Fiscal Year: 1999
    ..Finally, we describe two new projects that are now underway: the identification of MHC class I peptides associated with MMTV-infected mouse mammary tumor cells, and natural ligands recognized during T-cell selection in the thymus. ..
  68. NUCLEOPROTEINS IN MAMMALIAN SPERMATOGENESIS
    Marvin Meistrich; Fiscal Year: 2006
    ..Thus they provide a well-defined model system for study of human infertility. ..
  69. REGULATORY MECHANISMS IN PROTEIN BIOSYNTHESIS
    WLODZIMIERZ SZER; Fiscal Year: 1990
    ....
  70. Development of a HTS Assay Targeting End Protection of Telomeres
    DEBORAH WUTTKE; Fiscal Year: 2007
    ..Small molecules that specifically target telomeres will provide valuable tools for dissecting the mechanism of telomere function and lay the groundwork for the discovery of cancer therapeutics. [unreadable]..
  71. ADENOVIRUS NUCLEOPROTEINS
    SARAH FLINT; Fiscal Year: 1991
    ..described in this proposal are concerned with characterization of the structure and function of the nucleoproteins within which the adenovirus type 2 (Ad2) genome resides in virions and within infected cells...
  72. INHIBITORS OF KINASES REQUIRED FOR HIV-1 INFECTIVITY
    Mario Stevenson; Fiscal Year: 1999
    ..3. Utilize biochemical and genetic approaches to identify and clone kinases involved in phosphorylation of gag MA. It is anticipated that these studies will lead to the derivation of novel agents for athe treatment of HIV-1 infection. ..