Genomes and Genes
gag gene products
Summary: Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.
Publications263 found, 100 shown here
- Lentiviral RNAs can use different mechanisms for translation initiationEmiliano P Ricci
Unité de Virologie Humaine, Ecole Normale Superieure de Lyon, IFR 128, Lyon, F 69364, France
Biochem Soc Trans 36:690-3. 2008..Our results show that HIV-1 is able to drive the synthesis of the Gag polyprotein both by a classical cap-dependent mechanism and an IRES, whereas HIV-2 and SIV appear to use exclusively an IRES mechanism...
- CD8+ T-cell responses to different HIV proteins have discordant associations with viral loadPhotini Kiepiela
HIV Pathogenesis Programme, Doris Duke Medical Research Institute, University of KwaZulu Natal, Durban 4013, South Africa
Nat Med 13:46-53. 2007..These population-based data, suggesting the existence of both effective immune responses and responses lacking demonstrable biological impact in chronic HIV infection, are of relevance to HIV vaccine design and evaluation...
- AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus buddingBettina Strack
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
Cell 114:689-99. 2003..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
- Superior control of HIV-1 replication by CD8+ T cells is reflected by their avidity, polyfunctionality, and clonal turnoverJorge R Almeida
Cellular Immunology Laboratory, U543, Institut National de la Sante et de la Recherche Medicale, Avenir Group, 75013 Paris, France
J Exp Med 204:2473-85. 2007..Such attributes are interlinked and constitute the basis for effective control of HIV-1 replication. These data on the features of effective CD8+ T cells in HIV infection may aid in the development of successful T cell vaccines...
- The protein network of HIV buddingUta K von Schwedler
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
Cell 114:701-13. 2003..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses...
- The stoichiometry of Gag protein in HIV-1John A G Briggs
Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK
Nat Struct Mol Biol 11:672-5. 2004..In the immature virus, Gag forms a hexameric lattice with a spacing of 8.0 nm. Thus, less than half of the CA proteins form the mature core...
- Gag-specific CD8+ T lymphocytes recognize infected cells before AIDS-virus integration and viral protein expressionJonah B Sacha
Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
J Immunol 178:2746-54. 2007..0017) in SIV-infected rhesus macaques. These results suggest that HIV vaccines should focus CD8(+) T cell responses on Gag...
- Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 buddingJ E Garrus
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
Cell 107:55-65. 2001..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
- Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain functionDimiter G Demirov
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
Proc Natl Acad Sci U S A 99:955-60. 2002..These data demonstrate a link between the E2-like domain of TSG101 and HIV-1 L domain function, and indicate that TSG101 derivatives can act as potent and specific inhibitors of HIV-1 replication by blocking virus budding...
- AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assemblyXinhong Dong
Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
Cell 120:663-74. 2005..These studies illuminate an early step in retroviral particle assembly and provide evidence that the trafficking of Gag to late endosomes is part of a productive particle assembly pathway...
- Magnitude of functional CD8+ T-cell responses to the gag protein of human immunodeficiency virus type 1 correlates inversely with viral load in plasmaBradley H Edwards
Department of Medicine, University of Alabama, 845 19th Street South, BBRB 226, Birmingham, AL 35294, USA
J Virol 76:2298-305. 2002..These data serve as strong evidence that major histocompatibility complex class I presentation of Gag peptides is an essential feature for any HIV-1 vaccine designed to elicit optimal CD8+ T-cell responses...
- Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assemblyJamil S Saad
Howard Hughes Medical Institute, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
Proc Natl Acad Sci U S A 103:11364-9. 2006..Our findings indicate that PI(4,5)P(2) acts as both a trigger of the myristyl switch and a membrane anchor and suggest a potential mechanism for targeting Gag to membrane rafts...
- Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus buddingRobert D Fisher
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
Cell 128:841-52. 2007..ALIX therefore serves as a flexible, extended scaffold that connects retroviral Gag proteins to ESCRT-III and other cellular-budding machinery...
- HIV-1 viral escape in infancy followed by emergence of a variant-specific CTL responseMargaret E Feeney
Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
J Immunol 174:7524-30. 2005..Taken together, these findings indicate that the developing immune system of children may exhibit greater plasticity in responding to a continually evolving chronic viral infection...
- Virus population homogenization following acute human immunodeficiency virus type 1 infectionGerald H Learn
Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
J Virol 76:11953-9. 2002..This early selective process focuses on viral properties within Env but not Gag p17. Hence, the viral homogeneity observed early in HIV-1 infection results from a selective process that occurs during the establishment of infection...
- In vitro assembly properties of human immunodeficiency virus type 1 Gag protein lacking the p6 domainS Campbell
ABL Basic Research Program, NCI Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
J Virol 73:2270-9. 1999....
- Visualization of retroviral replication in living cells reveals budding into multivesicular bodiesNathan M Sherer
Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Ave, New Haven, CT 06536, USA
Traffic 4:785-801. 2003..These data suggest that retroviruses can interact with the vps sorting machinery in a more traditional sense, directly linked to the mechanism by which cellular proteins are sorted into multivesicular endosomes...
- Plasma membrane rafts play a critical role in HIV-1 assembly and releaseA Ono
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Room 307, 9000 Rockville Pike, Bethesda, MD 20892-0460, USA
Proc Natl Acad Sci U S A 98:13925-30. 2001..These results identify the association of Gag with plasma membrane rafts as an important step in HIV-1 replication. These findings may lead to novel strategies for suppressing HIV-1 replication in vivo...
- Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2Melissa Batonick
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
Virology 342:190-200. 2005..Together these data attribute a role to the AP-2 complex in the regulation of HIV-1 assembly/release...
- HLA-associated immune escape pathways in HIV-1 subtype B Gag, Pol and Nef proteinsZabrina L Brumme
Ragon Institute of MGH, MIT and Harvard, Charlestown, MA, USA
PLoS ONE 4:e6687. 2009..Results are organized into protein-wide escape maps illustrating the sites and pathways of HLA-driven viral evolution...
- Mutations of the human immunodeficiency virus type 1 p6Gag domain result in reduced retention of Pol proteins during virus assemblyX F Yu
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
J Virol 72:3412-7. 1998..These results suggest that the p6 domain of HIV-1 Gag may play an important role in recruiting or retaining cleaved Pol proteins during virus assembly...
- HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egressJ Martin-Serrano
Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
Nat Med 7:1313-9. 2001..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
- Functional replacement and positional dependence of homologous and heterologous L domains in equine infectious anemia virus replicationFeng Li
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
J Virol 76:1569-77. 2002....
- The late domain of human immunodeficiency virus type 1 p6 promotes virus release in a cell type-dependent mannerDimiter G Demirov
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
J Virol 76:105-17. 2002..The results described here define the role of p6 in virus replication in a wide range of cell types and reveal a strong cell type-dependent requirement for p6 in virus particle budding...
- Improved primate foamy virus vectors and packaging constructsMartin Heinkelein
Institut fur Virologie und Immunbiologie, Universitat Wurzburg, Germany
J Virol 76:3774-83. 2002..By this strategy, efficient vector transfer was achieved with constructs that have minimal genetic overlap...
- Tsg101 control of human immunodeficiency virus type 1 Gag trafficking and releaseA Goff
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794 5222, USA
J Virol 77:9173-82. 2003....
- Characterization of prototype foamy virus gag late assembly domain motifs and their role in particle egress and infectivityAnnett Stange
Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Fetscherstr 74, 01307 Dresden, Germany
J Virol 79:5466-76. 2005..Taken together these results demonstrate that PFV, like other viruses, requires components of the vacuolar protein sorting (VPS) machinery for egress and enters the VPS pathway through interaction with TSG101...
- T cell cross-reactivity and conformational changes during TCR engagementJean K Lee
Human Immunology Unit, Medical Research Council, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England, UK
J Exp Med 200:1455-66. 2004..These findings have implications for the rational design of vaccines targeting viruses with unstable genomes...
- The M-PMV cytoplasmic targeting-retention signal directs nascent Gag polypeptides to a pericentriolar region of the cellJeffrey N Sfakianos
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Traffic 4:660-70. 2003..Thus the previously defined cytoplasmic targeting-retention signal appears to act as a cotranslational intracellular targeting signal that concentrates Gag proteins at the centriole for assembly of capsids...
- Intracellular versus cell surface assembly of retroviral pseudotypes is determined by the cellular localization of the viral glycoprotein, its capacity to interact with Gag, and the expression of the Nef proteinVirginie Sandrin
INSERM U412, Lyon Ecole Normale Supérieure de Lyon, and IFR128 BioSciences Lyon Gerland, Lyon, F 69007 France
J Biol Chem 281:528-42. 2006..Finally, the expression of Nef proteins specifically enhanced the incorporation of the retroviral GPs by increasing their localization in late endosomes...
- Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membraneAmy M Booth
Department of Biological Chemistry and 2Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Cell Biol 172:923-35. 2006..In support of this, we find that Jurkat T cells direct the key budding factor of HIV, HIV Gag, to these endosome-like domains of plasma membrane and secrete HIV Gag from the cell in exosomes...
- HIV Gag mimics the Tsg101-recruiting activity of the human Hrs proteinOwen Pornillos
Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT 84132, USA
J Cell Biol 162:425-34. 2003..HIV-1 Gag apparently mimics this Hrs activity, and thereby usurps Tsg101 and other components of the MVB vesicle fission machinery to facilitate viral budding...
- Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle releaseH G Gottlinger
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115
Proc Natl Acad Sci U S A 88:3195-9. 1991..Inhibition of the release of the viral capsid proteins by interferon alpha indicates that this step of virus maturation may be sensitive to inhibition by antiviral drugs...
- HIV-1 nucleocapsid protein as a nucleic acid chaperone: spectroscopic study of its helix-destabilizing properties, structural binding specificity, and annealing activityMaria A Urbaneja
AIDS Vaccine Program, SAIC Frederick, Building 535 424, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
J Mol Biol 318:749-64. 2002....
- Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsidT R Gamble
Biochemistry Department, University of Utah, Salt Lake City 84103, USA
Cell 87:1285-94. 1996..Side by side association of these strips may allow capsid to form the surface of the viral core. Cyclophilin A could then function by weakening the association between capsid strips, thereby promoting disassembly of the viral core...
- The efficacy of DNA vaccination is enhanced in mice by targeting the encoded protein to dendritic cellsGodwin Nchinda
Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, New York 10065, USA
J Clin Invest 118:1427-36. 2008..The efficacy of DNA vaccines therefore may be enhanced by inclusion of sequences such as single-chain antibodies to target the antigen to DCs...
- Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cellsA Kikonyogo
Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 98:11199-204. 2001..These findings mechanistically link the cellular ubiquitination pathway to retrovirus budding...
- The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their buddingGregory Camus
Institut Cochin, Universite Paris Descartes, Centre National de la Recherche Scientifique UMR 8104, Paris, France
Mol Biol Cell 18:3193-203. 2007..We propose that AP-1 promotes Gag release by transporting it to intracellular sites of active budding, and/or by facilitating its interactions with other cellular partners...
- Transactivation of elements in the human endogenous retrovirus W family by viral infectionChristoffer Nellåker
The Department of Neuroscience, Karolinska Institutet, Retzius vag 8, 171 77 Stockholm, Sweden
Retrovirology 3:44. 2006....
- Contribution of CD8+ T cells to containment of viral replication and emergence of mutations in Mamu-A*01-restricted epitopes in Simian immunodeficiency virus-infected rhesus monkeysEun Young Kim
Division of Infectious Diseases, The Feinberg School of Medicine, Northwestern University, 676 N St Clair St, Suite 200, Chicago, IL 60611, USA
J Virol 82:5631-5. 2008..These results confirm the importance of cytotoxic T cells in controlling viremia and the constraint on epitope sequences that require compensatory changes to go to fixation...
- Infectious HIV-1 assembles in late endosomes in primary macrophagesAnnegret Pelchen-Matthews
Cell Biology Unit, Medical Research MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
J Cell Biol 162:443-55. 2003..This notion has significant implications for understanding the biology of HIV and its cell-cell transmission...
- Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: implications for the safety of lentiviral vectorsR Wagner
Institut für medizinische Mikrobiologie and Hygiene, Universitat Regensburg, D 93053 Regensburg, Germany
Hum Gene Ther 11:2403-13. 2000..By eliminating regions of homology and sequences involved in packaging, synthetic gag-pol genes should improve the safety profile of lentiviral vectors...
- On-going generation of multiple forms of HIV-1 intersubtype recombinants in the Yunnan Province of ChinaRongge Yang
Laboratory of Molecular Virology and Epidemiology, AIDS Research Center, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
AIDS 16:1401-7. 2002....
- Organization of immature human immunodeficiency virus type 1T Wilk
The Structural Biology Programme, European Molecular Biology Laboratory, D69012 Heidelberg, Federal Republic of Germany
J Virol 75:759-71. 2001..Authentic, immature HIV-1 displays additional surface features and an increased density between the lipid bilayers which reflect the presence of gp41. The other internal features match those of virus-like particles...
- HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRETEmma Poole
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, CB2 2QQ, UK
Traffic 6:741-55. 2005..HIV-1 Psi thus acts as a subcellular localisation signal as well as a high-affinity-binding site for Gag...
- Characterization and selection of HIV-1 subtype C isolates for use in vaccine developmentCarolyn Williamson
Division of Medical Virology, University of Cape Town, Observatory, Cape Town, South Africa 7925
AIDS Res Hum Retroviruses 19:133-44. 2003..Genes identified in this study have been incorporated into the VEE VRP candidate vaccines targeted for clinical trial in South Africa...
- Identification of a host protein essential for assembly of immature HIV-1 capsidsConcepcion Zimmerman
Department of Physiology, University of California at San Francisco, San Francisco, California 94143, USA
Nature 415:88-92. 2002..These findings support a critical role for HP68 in post-translational events of HIV-1 assembly and reveal a previously unappreciated dimension of host-viral interaction...
- Proteolytic processing of foamy virus Gag and Pol proteinsR M Flugel
Retroviral Gene Expression, Research Programme Applied Tumor Virology, German Cancer Research Center, Im Neuenheimer Feld 242, 69009 Heidelberg, Germany
Curr Top Microbiol Immunol 277:63-88. 2003..The temporal and spatial control and the factors that regulate FV PRs remain to be elucidated...
- PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101 [corrected]Fadila Bouamr
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York, USA
J Virol 77:11882-95. 2003..This result suggests that Nedd4 is involved early in budding of HTLV-1...
- Virus maturation by buddingH Garoff
Department of Biosciences at Novum, S 141 57 Huddinge, Sweden
Microbiol Mol Biol Rev 62:1171-90. 1998..This new concept also provides answers to the question of how viral and cellular membrane proteins are sorted during budding. In addition, it has implications for the mechanism by which the virion is uncoated during virus entry...
- Interaction of the human immunodeficiency virus type 1 nucleocapsid with actinB Liu
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
J Virol 73:2901-8. 1999....
- Entropic switch regulates myristate exposure in the HIV-1 matrix proteinChun Tang
Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 5398, USA
Proc Natl Acad Sci U S A 101:517-22. 2004..Our findings indicate that the HIV-1 myristyl switch is regulated not by mechanically induced conformational changes, as observed for other myristyl switches, but instead by entropic modulation of a preexisting equilibrium...
- The microbial mimic poly IC induces durable and protective CD4+ T cell immunity together with a dendritic cell targeted vaccineChristine Trumpfheller
Laboratory of Cellular Physiology and Immunology, Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Proc Natl Acad Sci U S A 105:2574-9. 2008..We suggest that poly IC be tested as an adjuvant with DC-targeted vaccines to induce numerous multifunctional CD4(+) Th1 cells with proliferative capacity...
- Elevated levels of human endogenous retrovirus-W transcripts in blood cells from patients with first episode schizophreniaY Yao
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Genes Brain Behav 7:103-12. 2008..05) in the patients compared with the controls. Thus, studies aiming to further understanding of complex human disease such as schizophrenia may need to be extended beyond the strictly protein-coding fraction of the transcriptome...
- APOBEC3G incorporation into human immunodeficiency virus type 1 particlesVeronique Zennou
Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
J Virol 78:12058-61. 2004..Because it is, therefore, difficult to evolve specific sequences that confer escape from APOBEC3G, these findings may explain why lentiviruses evolved an activity that induces its destruction...
- M-PMV capsid transport is mediated by Env/Gag interactions at the pericentriolar recycling endosomeJeffrey N Sfakianos
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Traffic 4:671-80. 2003..Thus, for the first time, endocytic trafficking of a retroviral Env glycoprotein is linked to the efficient cytoplasmic transport of Gag...
- Sequence-specific binding of human immunodeficiency virus type 1 nucleocapsid protein to short oligonucleotidesR J Fisher
Protein Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
J Virol 72:1902-9. 1998....
- Nedd4.1-mediated ubiquitination and subsequent recruitment of Tsg101 ensure HTLV-1 Gag trafficking towards the multivesicular body pathway prior to virus buddingVincent Blot
Departement de Biologie Cellulaire, CNRS UMR 8104 and INSERM U567, Institut Cochin, 75014 Paris, France
J Cell Sci 117:2357-67. 2004..Our findings indicate that Nedd4.1 and Tsg101 act successively in the assembly process of HTLV-1 to ensure proper Gag trafficking through the endocytic pathway up to late endosomes where the late steps of retroviral release occur...
- Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1Luciana J Costa
Molecular Virology Laboratory, Dep of Genetics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Retrovirology 3:33. 2006..In this report, we investigated further the ability of Nef to facilitate the replication of HIV-1...
- Heteronuclear NMR studies of the interaction of tRNA(Lys)3 with HIV-1 nucleocapsid proteinC Tisne
Laboratoire de Cristallographie et RMN Biologiques, EP 2075 CNRS Faculté de Pharmacie, 4 avenue de l Observatoire, Paris, 75006, France
J Mol Biol 306:443-54. 2001..It is interesting that for the biological role of the NC protein, these pairs could be the starting points of the tRNA melting required for the hybridisation to the viral RNA...
- Human endogenous retrovirus (HERV)-W ENV and GAG proteins: physiological expression in human brain and pathophysiological modulation in multiple sclerosis lesionsHervé Perron
bioMerieux, R and D, Chemin de l orme, 69280 Marcy l Etoile, France
J Neurovirol 11:23-33. 2005..This is compatible with a pathophysiological role in MS, but also illustrates the ambivalence of such HERV antigens, which can be expressed in cell-specific patterns, under physiological or pathological conditions...
- Role for HLA class II molecules in HIV-1 suppression and cellular immunity following antiretroviral treatmentU Malhotra
Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3-100, Seattle, WA 98109, USA
J Clin Invest 107:505-17. 2001..Eliciting responses to the promiscuous epitope region may be beneficial in vaccine strategies...
- Efficient processing of the immunodominant, HLA-A*0201-restricted human immunodeficiency virus type 1 cytotoxic T-lymphocyte epitope despite multiple variations in the epitope flanking sequencesC Brander
AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
J Virol 73:10191-8. 1999..These data suggest that escape from this immunodominant CTL response is not frequently accomplished by changes in the epitope flanking sequences...
- Escape in one of two cytotoxic T-lymphocyte epitopes bound by a high-frequency major histocompatibility complex class I molecule, Mamu-A*02: a paradigm for virus evolution and persistence?Thorsten U Vogel
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
J Virol 76:11623-36. 2002..Differential selection by CTL may therefore be a paradigm of immunodeficiency virus infection...
- Regulation of human endogenous retrovirus W protein expression by herpes simplex virus type 1: implications for multiple sclerosisKlemens Ruprecht
Department of Neurology, Clinical Research Unit for Multiple Sclerosis and Neuroimmunology, Wurzburg, Germany
J Neurovirol 12:65-71. 2006....
- Evidence for a new viral late-domain core sequence, FPIV, necessary for budding of a paramyxovirusAnthony P Schmitt
Northwestern University, 2205 Tech Dr, Evanston, IL 60208 3500, USA
J Virol 79:2988-97. 2005..We hypothesize that these proline residues act to partially restore virus budding by generation of new motifs that act as suboptimal late domains...
- Evaluation of minor groove binding probe and Taqman probe PCR assays: Influence of mismatches and template complexity on quantificationYuanrong Yao
Department of Neuroscience, Karolinska Institute, Retzius vag 8, S 171 77 Stockholm, Sweden
Mol Cell Probes 20:311-6. 2006....
- A role for ubiquitin ligase recruitment in retrovirus releaseB Strack
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 97:13063-8. 2000..Our findings suggest that the engagement of the ubiquitin conjugation machinery by L domains plays a crucial role in the release of a diverse group of enveloped viruses...
- Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytesJ E Schmitz
Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Science 283:857-60. 1999..These results confirm the importance of cell-mediated immunity in controlling HIV-1 infection and support the exploration of vaccination approaches for preventing infection that will elicit these immune responses...
- Nucleic acid-independent retrovirus assembly can be driven by dimerizationMarc C Johnson
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
J Virol 76:11177-85. 2002....
- Studies on the mechanism of inactivation of the HIV-1 nucleocapsid protein NCp7 with 2-mercaptobenzamide thioestersLisa M Miller Jenkins
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
J Med Chem 48:2847-58. 2005..These studies provide a better understanding of the mechanism of action of thioester compounds, which is important for future design of anti-HIV-1 compounds that target NCp7...
- Role of murine leukemia virus nucleocapsid protein in virus assemblyDelphine Muriaux
HIV Drug Resistance Program, National Cancer Institute Frederick, P O Box B, Frederick, MD 21702 1201, USA
J Virol 78:12378-85. 2004..Therefore, the functions of NC in virus budding and infectivity are completely distinct from viral late-domain function...
- Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15Atsushi Okumura
The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 103:1440-5. 2006..Identification of ISG15 as the critical component in IFN-mediated inhibition of HIV-1 release advances the understanding of the IFN-mediated inhibition of HIV-1 replication and uncovers a target for the anti HIV-1 therapy...
- Human immunodeficiency virus type 1 assembly, release, and maturationCatherine S Adamson
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
Adv Pharmacol 55:347-87. 2007
- Replication-incompetent adenoviral vaccine vector elicits effective anti-immunodeficiency-virus immunityJohn W Shiver
Merck Research Laboratories, West Point, Pennsylvania 19486, USA
Nature 415:331-5. 2002..The replication-defective adenovirus is a promising vaccine vector for development of an HIV-1 vaccine...
- Affinities of the nucleocapsid protein for variants of SL3 RNA in HIV-1Andrew C Paoletti
Department of Chemistry, Graduate Program in Structural Biology, Biochemistry, and Biophysics, Syracuse University, Syracuse, New York 13244 4100, USA
Biochemistry 41:15423-8. 2002..These results are interesting in the context of RNA-protein interaction, as well as for the discovery of antiNC agents for AIDS therapy...
- Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and BJ Luban
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032
Cell 73:1067-78. 1993..The Gag-CyP interaction may be important for the HIV-1 life cycle and may be relevant to the pathology caused by this immunosuppressive virus...
- Murine leukemia virus particle assembly quantitated by fluorescence microscopy: role of Gag-Gag interactions and membrane associationMariam Andrawiss
Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Science, University College London, London, United Kingdom
J Virol 77:11651-60. 2003..These data suggest that two independent mechanisms, CA interactions and membrane association following myristylation, cooperate in MLV Gag assembly and budding...
- Transmission of HIV-1 Gag immune escape mutations is associated with reduced viral load in linked recipientsPaul A Goepfert
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Exp Med 205:1009-17. 2008..In addition to their direct implications for HIV-1 vaccine design, these data suggest that CTL-induced viral polymorphisms and their associated in vivo viral fitness costs could have a significant impact on HIV-1 pathogenesis...
- Functional relationship between the matrix proteins of feline and simian immunodeficiency virusesMariana L Manrique
Centro de Virologia Animal CEVAN CONICET, C1414DEM Buenos Aires, Argentina
Virology 329:157-67. 2004..Our results thus provide novel information about the functional homology between the MA proteins of distantly related lentiviruses...
- Mutational analysis of the feline immunodeficiency virus matrix proteinM L Manrique
, Serrano 669, C1414DEM, Buenos Aires, Argentina
Virus Res 76:103-13. 2001..Our results reveal the role that the FIV MA plays in virus morphogenesis and contribute to the understanding of the assembly process in non-primate lentiviruses...
- The late-domain-containing protein p6 is the predominant phosphoprotein of human immunodeficiency virus type 1 particlesBarbara Muller
Abteilung Virologie, Universitatsklinikum Heidelberg, D 69120 Heidelberg, Germany
J Virol 76:1015-24. 2002..Inhibition experiments suggested that a cyclin-dependent kinase or a related kinase, most likely ERK2, was involved in p6 phosphorylation by virion-associated enzymes...
- Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag)L VerPlank
Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, NY 11794 5222, USA
Proc Natl Acad Sci U S A 98:7724-9. 2001..The results link L-domain function in HIV to the Ub machinery and a specific component of the cellular trafficking apparatus...
- Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membraneAkira Ono
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
Proc Natl Acad Sci U S A 101:14889-94. 2004..These results demonstrate that PI(4,5)P2 plays a key role in Gag targeting to the plasma membrane and thus serves as a cellular determinant of HIV-1 particle production...
- How retroviruses select their genomesVictoria D'Souza
Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA
Nat Rev Microbiol 3:643-55. 2005....
- Mechanism of nucleocapsid protein catalyzed structural isomerization of the dimerization initiation site of HIV-1Manuela J Rist
Center for Advanced Research in Biotechnology of the University of Maryland, Rockville 20850, USA
Biochemistry 41:14762-70. 2002....
- Dendritic cell-lysosomal-associated membrane protein (LAMP) and LAMP-1-HIV-1 gag chimeras have distinct cellular trafficking pathways and prime T and B cell responses to a diverse repertoire of epitopesLuciana B Arruda
Department of Pharmacology and Molecular Sciences, The Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
J Immunol 177:2265-75. 2006..These data indicate that LAMP-1 and DC-LAMP Ag chimeras follow different trafficking pathways, induce distinct modulatory immune responses, and are able to present cryptic epitopes...
- Retroviral genomic RNAs are transported to the plasma membrane by endosomal vesiclesEugenia Basyuk
IGMM CNRS UMR5535, Universite Montpellier II, IFR 24, 1919, route de Mende, 34293 Cedex 5, Montpellier, France
Dev Cell 5:161-74. 2003..This may allow retroviruses to hijack the endosomal machinery as part of their biosynthetic pathway. More generally, tethering to vesicles may provide an efficient mechanism for directed RNA transport...
- HIV-1 Gag protein associates with F-actin present in microfilamentsO Rey
Department of Pediatrics, School of Medicine, University of California at Los Angeles 90095, USA
Virology 220:530-4. 1996..In vivo and in vitro analyses of this interaction indicated that the unprocessed Gag polyprotein is capable of association with polymerized actin (F-actin). Binding of Gag to F-actin may be involved in the assembly or budding of HIV-1...
- Foamy virus Bet proteins function as novel inhibitors of the APOBEC3 family of innate antiretroviral defense factorsRebecca A Russell
Jefferiss Trust Laboratories, Wright Fleming Institute, Imperial College, London, United Kingdom
J Virol 79:8724-31. 2005..While these data identify the foamy virus Bet protein as a functional ortholog of the HIV-1 Vif auxiliary protein, they also indicate that Vif and Bet block APOBEC3 protein function by distinct mechanisms...
- Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shellsElizabeth R Wright
Division of Biology, California Institute of Technology, Pasadena, CA, USA
EMBO J 26:2218-26. 2007..This model suggests why the SP1 spacer is essential for assembly of the Gag lattice and how cleavage between SP1 and CA acts as a structural switch controlling maturation...
- HIV type 1 Gag and nucleocapsid proteins: cytoskeletal localization and effects on cell motilityF J Ibarrondo
Department of Pediatrics, UCLA School of Medicine, Los Angeles, California 90095, USA
AIDS Res Hum Retroviruses 17:1489-500. 2001..These data suggest that interactions between HIV-1 Gag and actin in infected cells enhance cell motility. Ultimately this enhanced motility of infected cells could promote the dissemination of virus into the brain and other tissues...
- Analysis of total human immunodeficiency virus (HIV)-specific CD4(+) and CD8(+) T-cell responses: relationship to viral load in untreated HIV infectionM R Betts
Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9113, USA
J Virol 75:11983-91. 2001..These results suggest that overall frequencies of HIV-specific T cells are not the sole determinant of immune-mediated protection in HIV-infection...
- Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retrovirusesVirginie Sandrin
Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, IFR128 Biosciences Lyon Gerland, Ecole Normal Supérieure de Lyon, France
J Virol 78:7153-64. 2004..Thus, intracellular colocalization, as well as interactions between Env and core proteins, may influence the recruitment of the glycoprotein onto viral particles and generate infectious pseudotyped viruses...
- Retrovirus infection strongly enhances scrapie infectivity release in cell culturePascal Leblanc
LaboRetro Unité de virologie humaine INSERM U758, Ecole Normale Superieure de Lyon, Lyon Cedex, France
EMBO J 25:2674-85. 2006..Under these conditions, we found that PrPC, PrPSc and scrapie infectivity are recruited by both MuLV virions and exosomes. We propose that retroviruses can be important cofactors involved in the spread of the pathological prion agent...
- Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during buddingCarolina Segura-Morales
IGMM CNRS, 1919 Route de Mende 34293, Montpellier Cedex 5, France
J Biol Chem 280:27004-12. 2005..1. Other Nedd4-dependent Gag proteins also contain binding sites for Tsg101 or Alix, suggesting that this could be a common feature of retroviruses...
- HIV-1 assembly and maturationA G Bukrinskaya
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, U S A
Arch Virol 149:1067-82. 2004..This brief review summarizes some recent findings on the final steps of the HIV-1 life cycle and touches upon some unanswered questions, particularly regarding the processes involved in virus maturation and infectivity...
- Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1Cagan Gurer
Department of Microbiology, Columbia University, 701 W 168th St, New York, NY 10032, USA
J Virol 79:910-7. 2005..HIV-1 bearing the p6-K27R mutation was insensitive to SUMO-1 overexpression, suggesting that covalent attachment of SUMO-1 to p6 is detrimental to HIV-1 replication...
- HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membraneDmitriy Mazurov
National Cancer Institute, HIV Drug Resistance Program, Bld 535, Rm 110, NCI Frederick, Frederick, MD 21702 1201, USA
Virology 346:194-204. 2006..Our data suggest that once at the plasma membrane, HTLV-1 virion components associate with CD82-containing microdomains, which may facilitate the mobilization of nascent virions to sites of intercellular adhesion...
- Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopyDaniel R Larson
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
J Cell Biol 162:1233-44. 2003..These methods also have general applicability to in vivo studies of protein-protein and -membrane interactions involved in the formation of complex macromolecular structures...
- The critical role of proximal gag sequences in feline immunodeficiency virus genome encapsidationIris Kemler
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Virology 327:111-20. 2004..Focusing further on this proximal sequence, we found that a deletion of only 13 nt at the 5' end of gag impaired encapsidation of subgenomic vector and proviral RNAs...
- Immunization Strategies for Autologous HIV ImmunotherapyLouis D Falo; Fiscal Year: 2012..The studies we propose include translational preclinical models designed as a direct prelude to human clinical trials. ..
- Monitoring single conformational events during HIV assemblyWALTHER H MOTHES; Fiscal Year: 2013..A detailed knowledge of the energy landscape and the kinetics of HIV assembly will aid in the identification of novel structural intermediates. This new information will be relevant for the rational design of antiviral therapies. ..
- Rational Design of antivrials targeted to HIV-1 capsidAsim K Debnath; Fiscal Year: 2013..The studies described in this proposal may lead to the development of a new class of antiretroviral therapeutics targeting the HIV-1 capsid. ..
- Viral and Cellular Determinants of HIV-1 AssemblyPaul W Spearman; Fiscal Year: 2013....
- Dendritic Cell-Targeted HIV Vaccine ProductTibor Keler; Fiscal Year: 2011....
- Membrane Coupling and Dynamic Reorganization of Gag in Viral BuddingMATHIAS LOESCHE; Fiscal Year: 2013..Its broader impact is to provide new techniques for the structural characterization of membrane proteins associated with lipid bilayers in their physiologically relevant, thermally disordered state. ..
- A New Method for Biomembrane SimulationsGregory A Voth; Fiscal Year: 2012....
- High-throughput screening for HIV assembly and maturation inhibitorsWuyuan Lu; Fiscal Year: 2013..Our long-term objective is to develop HIV assembly and maturation inhibitors as a novel class of therapeutic agents for the prevention of HIV-1 infection and the treatment of AIDS. ..
- NMR STUDIES OF HIV-1 PROTEINSMICHAEL FINLEY SUMMERS; Fiscal Year: 2013..abstract_text> ..
- HIV Nucleocapsid Protein Nucleic Acid Chaperone ActivityKARIN M MUSIER-FORSYTH; Fiscal Year: 2013..The specific aims are: (1) To probe the NA chaperone activity of WT, mutant and precursor forms of HIV-1 NC, and (2) to probe HIV-1 Gag's chaperone activity in vitro and in vivo. ..
- Structural Basis for Long-Range Genetic Interactions in Retroviral CA AssemblyRebecca Craven; Fiscal Year: 2009..A detailed understanding of this essential step of virus infection will allow development of better maturation inhibitors for use as antiretroviral drugs. ..
- MOUSE RETROVIRUS CO-OPTING OF IMMUNE CELL INTERACTIONSWilliam Green; Fiscal Year: 2005..abstract_text> ..
- TNT: Targeting and Triggering Adjuvancy of DNA VaccinesMichele Kutzler; Fiscal Year: 2005..Taken together, this proposal aims to identify and characterize a unique TNT adjuvant combination for HIV-1 DNA vaccines resulting in enhanced antigen specific cell-mediated immune responses and improved HIV-1 DNA vaccine candidates. ..
- MOLECULAR BIOLOGY OF HIV1 GAG PROTEINSHeinrich Gottlinger; Fiscal Year: 2003..An understanding of the molecular requirements for the association of these viral and cellular proteins with HIV-1 virions could provide specific targets for anti-viral therapy. ..
- GENETICS OF PRIMATE D TYPE RETROVIRUSESEric Hunter; Fiscal Year: 1999..The carboxy-terminal amino acid sequence of the five major gag gene products of this virus will be determined...
- GENETICS OF PRIMATE D TYPE RETROVIRUSESEric Hunter; Fiscal Year: 2001..Screen for inhibitors of the proteinase using a combinatorial peptide library; 2d. Crystallize and determine the structure of the different forms of the proteinase. ..
- HIV-1 Gag structure as a therapeutic targetMamuka Kvaratskhelia; Fiscal Year: 2007..Potential outcome of these studies include development of a new method that could be exploited for screening of larger chemical libraries to discover novel potent anti-assembly inhibitors. [unreadable] [unreadable] [unreadable]..
- MOLECULAR ANALYSIS OF HIV REPLICATIONMaxine Linial; Fiscal Year: 1992....
- NONREPLICATING MODIFIED VIRUS LIKE PARTICLES (VLPS) VACPolly Roy; Fiscal Year: 1999..Once optimal production method for VLPs is established, VLPs presenting all clades of HIV-1 Env and Gag antigens will be generated using baculovirus multigene expression system. ..
- KIF4 motor protein, regulates trafficking and stability of HIV-1 GagNathaniel W Martinez; Fiscal Year: 2010..Gag trafficking and virus particle assembly remains an unexplored field for targeting &development of novel pharmaceutical inhibitors needed to treat HIV infections. ..
- ANALYSIS OF RETROVIRUS ASSEMBLY BY IN VITRO MUTAGENESISJohn Wills; Fiscal Year: 1990..The long-range goal of the work proposed here is to elucidate the roles of the gag gene products in the assembly of Rous sarcoma virus (RSV), the prototype retrovirus...
- A computer model for the membrane binding of HIV-1 GagDiana Murray; Fiscal Year: 2003..abstract_text> ..
- HUMAN FOAMY VIRUS STRUCTURAL PROTEINSPaul Goepfert; Fiscal Year: 2000..A better understanding of the human member of this remarkable but relatively neglected genus of retroviruses will enhance our knowledge of both viral and cellular mechanisms. ..
- HOST CELL PROTEINS IN RETROVIRAL ASSEMBLY & TRANSPORTRachel LaCasse; Fiscal Year: 2002..abstract_text> ..
- Immunogenicity of HSV Amplicons in Rhesus MacaquesLuis Giavedoni; Fiscal Year: 2004..In addition, the successful completion of these studies will have a significant impact on the continued development of HSV amplicons as gene and cancer therapy vectors. ..
- BIOPHYSICAL STUDIES OF HIV ASSEMBLY AND MATURATIONPeter E Prevelige; Fiscal Year: 2010..abstract_text> ..
- The Role of Matrix Cofactors in SIVcpz ReplicationFrederic Bibollet Ruche; Fiscal Year: 2009..The knowledge gained from this project and the SIVcpz-chimpanzee model can be used to expand our understanding of HIV-1 MA protein interactions and could lead to new approaches for treatment of HIV-1 in patients. ..
- Intracellular Localization of HIV-1 RNAs by Rev&MatrixMichael Green; Fiscal Year: 2005..Finally, our results suggest a novel method for inhibiting the activity of any mRNA. This "mRNA nuclear entrapment" strategy will be developed and tested using HIV-1 replication as a model system. ..
- EIAV Control by High Avidity Rev-Specific CTL ClonesROBERT MEALEY; Fiscal Year: 2005..The information obtained from the proposed studies should have implications for HIV-1 immunotherapy and vaccine design, where experiments of this type may be more difficult. ..
- HIV-1 Suppression by MHC class I restricted CD8 T CellsPaul Goepfert; Fiscal Year: 2008..Our findings will have important implications fro HIV immunopathogenesis and vaccine design by supplying an HIV-1 specific CD8 T cell phenotype that results in efficient HIV-1 control. [unreadable] [unreadable] [unreadable]..
- Structural Biology of HIV Assembly, Budding, and EntryWesley Sundquist; Fiscal Year: 2006..In summary, our Program will provide significant insights into how the HIV virion functions as a machine that can enter and exit cells, and how these processes can be inhibited. ..
- STRUCTURE AND FUNCTION OF HIV GAG PROTEINMARILYN RESH; Fiscal Year: 2009..Multicolor imaging with site-specific fluorescein derivatives (FLASH and ReAsH) will be used to determine the order in which Gag assembly sites are formed and maintained in the cell. ..
- AN EVOLUTIONARY LINK BETWEEN TELOMERES AND TRANSPOSONSMARY LOU PARDUE; Fiscal Year: 2008..We have discovered that HeT-A and TART expression can be regulated by several external agents. We describe experiments to better define the set of agents capable of this regulation and to explore possible mechanisms. ..
- Regulation of APOBEC3G enzymatic activity in HIV-infected primary human T cellsJAISRI LINGAPPA; Fiscal Year: 2008..abstract_text> ..
- MVA/HIV-48: Potential Dual Vaccine for HIV and SmallpoxRama Rao Amara; Fiscal Year: 2003..The project will be a collaborative undertaking between researchers at the CDC, the NIAID, and the NIAID HIV Vaccine Trial Network (HVTN ). The focus of this application is the quantification and phenotyping of responding T cells. ..
- STRUCTURE/FUNCTION OF HIV1 CAPSID/CYCLOPHILIN COMPLEXWesley Sundquist; Fiscal Year: 2001..Finally, genetic analysis of mutants of CA in an HIV provirus will be used to test models that arise from the CA and CA-cyclophilin structures. ..
- ALABAMA/ZAMBIA HIV VACCINE CLINICAL TRIALS UNIT (HVTU)Paul Goepfert; Fiscal Year: 2006..Recently, the Alabama clinic enrolled 125 higher-risk gay men in <5 months in the first US phase III trial. Plans for utilizing these cohorts and others are described. ..
- Poly-functional analyses of vaccine-induced T cell responsesGuido Ferrari; Fiscal Year: 2010..Overall, these findings will shed new insights on the importance and fate of vaccine-induced T cell responses in controlling HIV-infection. ..
- AN EVOLUTIONARY LINK BETWEEN TELOMERES AND TRANSPOSONSMARY LOU PARDUE; Fiscal Year: 2006..abstract_text> ..
- EIAV Control by DNA Vaccine-Induced CTLROBERT MEALEY; Fiscal Year: 2006..The information obtained from the proposed studies should have implications for HIV-1 vaccine design, where experiments of this type may be more difficult. [unreadable] [unreadable]..
- Glial cell differentiation and glioma formationMARILYN RESH; Fiscal Year: 2006..The activity of the above proteins in human glioma tumor samples will be determined. ..
- Immune Control of HIV in ChildrenMargaret Feeney; Fiscal Year: 2006..This research will be conducted under the guidance of leading investigators in the field of HIV immunopathogenesis in adults. ..
- PROTEINS OF TELOMERE RETROTRANSPOSABLE ELEMENTSMARY PARDUE; Fiscal Year: 2001....
- Role of Lysyl-tRNA Synthetase in HIV-1 ReplicationLawrence Kleiman; Fiscal Year: 2006..Both biochemical purification methods and a genetic strategy for identifying cDNAs coding for LysRS proteases will be used to identify cellular proteases that can cleave LysRS. ..
- Cell-free VEE assembly system and alphavirus drug screenJAISRI LINGAPPA; Fiscal Year: 2006..The cell-free VEE assembly system offer a safe and low cost approach to understanding the mechanism of VEE capsid formation and to screen for agents active against alphaviruses that have potential as bioterrorism agents. ..
- Mucosal and Peripheral NK-T Cells in HIV InfectionOtto Yang; Fiscal Year: 2007..Exploring NK-T cells in HIV-1 infection could there have a high impact on the development of new strategies in prevention and treatment. [unreadable] [unreadable]..
- CD8+ T cell cytokine production in HIV-1 infectionPaul Goepfert; Fiscal Year: 2005..An HIV-1 vaccine would also be enhanced by identifying CD8+ T cell epitopes that portend a favorable prognosis and are difficult for the virus to mutate (specific aims 2 and 3). ..
- TNFSF APC activators for HIV VaccinesRICHARD KORNBLUTH; Fiscal Year: 2003..Upon the completion of these feasibility studies, it will be clear if these concepts should be advanced into more extensive vaccine studies both for HIV and other pathogens. ..
- The impact of early antiretroviral therapy on HIV persistence and inflammationSTEVEN GRANT DEEKS; Fiscal Year: 2010..Our work may also provide important insights in the role of chronic inflammation in driving viral persistence, and hence may lead to the development of novel interventions. ..
- HIV Vaccine DevelopmentDavid Montefiori; Fiscal Year: 2003....
- CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTSStephen Goff; Fiscal Year: 2003..In each case, these tests will be applied to existing panels of mutants to help localize the regions needed for these activities. ..
- FOAMY VIRUS INFECTION IN VITRO AND IN VIVOMaxine Linial; Fiscal Year: 2005..These experiments will be will be important for the assessment of the utility of foamy viruses as vectors for gene therapy. ..
- Latent virus and HIV-1 specific immunity in LTNPsJoel Blankson; Fiscal Year: 2006..Siliciano, and the supportive environment of the Johns Hopkins University will provide Dr. Blankson with the skills he needs to develop into an independent clinician and basic scientist researcher in HIV-1 pathogenesis. ..
- A hybrid 12T Q-FTMS for the investigation of nucleic acids non-cov./cov.adductsDaniele Fabris; Fiscal Year: 2006..unreadable] [unreadable] [unreadable] [unreadable]..
- Implications of HIV in Low HCV Clearance in Chinese IDUsXiao Fang Yu; Fiscal Year: 2007..The results of these studies should provide further insight into the mechanism of HCV clearance and the development of immunity which are critical for the development of effective vaccine and new treatment strategies. ..
- MOLECULAR ANALYSIS OF A HIGHLY PATHOGENIC FIVJohn Elder; Fiscal Year: 2008..abstract_text> ..
- Dissecting HIV integration vs production in CD4+ T cellsUNA O DOHERTY; Fiscal Year: 2008..We will then apply what we learn from our in vitro studies to define more precisely the phenotype(s) of the CD4+ resting T cells that are latently infected in patients. ..
- Effect of Schistosoma Co-infection on HIV Immune ResponsesHuyen Cao; Fiscal Year: 2007..mansoni infection dysregulates cellular immune responses by enhancing the activity of IL-10-producing T cells. S. mansoni co-infection will therefore be examined in the context of being an experimental immune modulator. ..
- Cell immune responses in SIV-infected sooty mangabeysAmitinder Kaur; Fiscal Year: 2004..abstract_text> ..
- VACCINE-ELICITED SIV-SPECIFIC CTLWILLIAM CHARINI; Fiscal Year: 2004..In addition, a committee of distinguished scientists including two AIDS researchers and an immunologist, will oversee Dr. Charini's progress. ..
- Immunologic Control of Drug-resistant HIV-1Steven Deeks; Fiscal Year: 2005..Finally, since we will estimate the relative in vivo thresholds for HIV-mediated immunogenicity and pathogenicity, these studies may also have implications for vaccine development. ..