hn protein

Summary

Summary: Glycoprotein from Sendai, para-influenza, Newcastle Disease, and other viruses that participates in binding the virus to cell-surface receptors. The HN protein possesses both hemagglutinin and neuraminidase activity.

Top Publications

  1. pmc Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant
    R A Karron
    Center for Immunization Research, Department of International Health, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:13961-6. 1997
  2. pmc Fusion activation by a headless parainfluenza virus 5 hemagglutinin-neuraminidase stalk suggests a modular mechanism for triggering
    Sayantan Bose
    Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 109:E2625-34. 2012
  3. pmc Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate
    Stephane Biacchesi
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892 8007, USA
    J Virol 78:12877-87. 2004
  4. pmc Structure and mutagenesis of the parainfluenza virus 5 hemagglutinin-neuraminidase stalk domain reveals a four-helix bundle and the role of the stalk in fusion promotion
    Sayantan Bose
    Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    J Virol 85:12855-66. 2011
  5. pmc Structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk
    Ping Yuan
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:14920-5. 2011
  6. pmc Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion
    Toru Takimoto
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    J Virol 76:13028-33. 2002
  7. pmc Membrane fusion machines of paramyxoviruses: capture of intermediates of fusion
    C J Russell
    Howard Hughes Medical Institute and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2153 N. Campus Drive, Evanston, IL 60208-3500, USA
    EMBO J 20:4024-34. 2001
  8. ncbi Sequence variation in the Newcastle disease virus genome
    Jacqueline A Kattenbelt
    CSIRO Livestock Industries, Australian Animal Health Laboratory, P O Bag 24, Geelong, Victoria 3220, Australia
    Virus Res 116:168-84. 2006
  9. ncbi Antitumor vaccination by Newcastle Disease Virus Hemagglutinin-Neuraminidase plasmid DNA application: changes in tumor microenvironment and activation of innate anti-tumor immunity
    Jing Ni
    Division of Cellular Immunology, German Cancer Research Center, Heidelberg, Germany
    Vaccine 29:1185-93. 2011
  10. pmc Characterization of a recombinant Newcastle disease virus vaccine strain
    Sun Hee Cho
    Zoonotic Disease Institute, Seoul National University, San 56 1, Shillim Dong, Gwanak Gu, Seoul, Korea
    Clin Vaccine Immunol 15:1572-9. 2008

Detail Information

Publications165 found, 100 shown here

  1. pmc Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant
    R A Karron
    Center for Immunization Research, Department of International Health, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 94:13961-6. 1997
    ..The requirement of SH and G for efficient replication in vivo suggests that selective deletion of one or both of these RSV genes may provide an alternative or additive strategy for developing an optimally attenuated vaccine candidate...
  2. pmc Fusion activation by a headless parainfluenza virus 5 hemagglutinin-neuraminidase stalk suggests a modular mechanism for triggering
    Sayantan Bose
    Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 109:E2625-34. 2012
    ..We provide evidence that the receptor-binding globular head domain of the paramyxovirus parainfluenza virus 5 HN protein is entirely dispensable for F activation...
  3. pmc Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate
    Stephane Biacchesi
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892 8007, USA
    J Virol 78:12877-87. 2004
    ..This indicates that SH is completely dispensable in vivo and that its deletion does not confer an attenuating effect, at least in this rodent model...
  4. pmc Structure and mutagenesis of the parainfluenza virus 5 hemagglutinin-neuraminidase stalk domain reveals a four-helix bundle and the role of the stalk in fusion promotion
    Sayantan Bose
    Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    J Virol 85:12855-66. 2011
    ..The multifunctional HN protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin ..
  5. pmc Structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk
    Ping Yuan
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:14920-5. 2011
    ..Two of four NA domains interact with the 4HB stalk, and residues at this interface in both the stalk and NA domain have been implicated in HN function...
  6. pmc Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion
    Toru Takimoto
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    J Virol 76:13028-33. 2002
    ..Our results suggest that the binding of HN protein to the receptor induces the conformational change of residues near the hydrophobic surface of HN protein and ..
  7. pmc Membrane fusion machines of paramyxoviruses: capture of intermediates of fusion
    C J Russell
    Howard Hughes Medical Institute and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2153 N. Campus Drive, Evanston, IL 60208-3500, USA
    EMBO J 20:4024-34. 2001
    ..The data are consistent with C-1 binding a pre-hairpin intermediate of F and with helical bundle formation being coupled directly to membrane fusion...
  8. ncbi Sequence variation in the Newcastle disease virus genome
    Jacqueline A Kattenbelt
    CSIRO Livestock Industries, Australian Animal Health Laboratory, P O Bag 24, Geelong, Victoria 3220, Australia
    Virus Res 116:168-84. 2006
    ....
  9. ncbi Antitumor vaccination by Newcastle Disease Virus Hemagglutinin-Neuraminidase plasmid DNA application: changes in tumor microenvironment and activation of innate anti-tumor immunity
    Jing Ni
    Division of Cellular Immunology, German Cancer Research Center, Heidelberg, Germany
    Vaccine 29:1185-93. 2011
    ..mammary carcinoma DA3 tumor model in comparison to application of a control plasmid not encoding the HN protein. Analysis of the tumor microenvironment revealed a significant increase in NK cell infiltration and decrease in ..
  10. pmc Characterization of a recombinant Newcastle disease virus vaccine strain
    Sun Hee Cho
    Zoonotic Disease Institute, Seoul National University, San 56 1, Shillim Dong, Gwanak Gu, Seoul, Korea
    Clin Vaccine Immunol 15:1572-9. 2008
    ..The HN protein length was increased from 571 to 577 as a marker...
  11. pmc The second receptor binding site of the globular head of the Newcastle disease virus hemagglutinin-neuraminidase activates the stalk of multiple paramyxovirus receptor binding proteins to trigger fusion
    Matteo Porotto
    Departments of Pediatrics and of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York, USA
    J Virol 86:5730-41. 2012
    ..We suggest a general model for paramyxovirus fusion activation in which receptor engagement at site II plays an active role in F activation...
  12. pmc Structure of the cleavage-activated prefusion form of the parainfluenza virus 5 fusion protein
    Brett D Welch
    Howard Hughes Medical Institute and Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 109:16672-7. 2012
    ..The conformational freedom of the charged arginine residues that compose the protease recognition site increases on cleavage of F protein...
  13. pmc Structure of the ulster strain newcastle disease virus hemagglutinin-neuraminidase reveals auto-inhibitory interactions associated with low virulence
    Ping Yuan
    Department of Structural Biology, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS Pathog 8:e1002855. 2012
    ..These results clarify how the Ulster HN C-terminal residues lead to an auto-inhibited state of HN, the requirement for proteolytic activation of HN₀ and associated reduced virulence...
  14. pmc Addition of N-glycans in the stalk of the Newcastle disease virus HN protein blocks its interaction with the F protein and prevents fusion
    Vanessa R Melanson
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0122, USA
    J Virol 80:623-33. 2006
    ..Our data support the idea that the F-interactive site on HN is defined by the stalk region of the protein...
  15. pmc Regulation of paramyxovirus fusion activation: the hemagglutinin-neuraminidase protein stabilizes the fusion protein in a pretriggered state
    Matteo Porotto
    Department of Pediatrics, Weill Medical College of Cornell University, New York, New York, USA
    J Virol 86:12838-48. 2012
    ..The results indicate that HN helps stabilize the prefusion state of F, and analysis of a stalk domain mutant HN reveals that the stalk domain of HN mediates the F-stabilization effect...
  16. pmc Analysis of parainfluenza virus-5 hemagglutinin-neuraminidase protein mutants that are blocked in internalization and degradation
    Jessica G Robach
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    Virology 406:189-201. 2010
    ..Furthermore, oligomeric analyses indicate that HN E37 mutants perturb the tetrameric organization of HN, probably by destabilizing the dimer-of-dimers interface...
  17. ncbi Localization of a new neutralizing epitope on the mumps virus hemagglutinin-neuraminidase protein
    M G Cusi
    Department of Molecular Biology, Section of Microbiology, University of Siena, Via Laterina, 8 53100, Siena, Italy
    Virus Res 74:133-7. 2001
    ..The peptide could, therefore, contain a new epitope, which may be critical for protective host humoral immune response...
  18. ncbi Sendai virus particle production: basic requirements and role of the SYWST motif present in HN cytoplasmic tail
    Anne Sophie Gosselin-Grenet
    Biologie Intégrative et Virologie des Insectes, INRA UMR1231, Universite Montpellier II, F 34000 Montpellier France
    Virology 405:439-47. 2010
    Sendai virus (SeV) HN protein is dispensable for virus particle production. HN incorporation into virions strictly depends on a cytoplasmic domain SYWST motif...
  19. ncbi Induction of interferon-alpha and tumor necrosis factor-related apoptosis-inducing ligand in human blood mononuclear cells by hemagglutinin-neuraminidase but not F protein of Newcastle disease virus
    Jinyang Zeng
    German Cancer Research Center, Division of Cellular Immunology, 69120 Heidelberg, Germany
    Virology 297:19-30. 2002
    ..Upon contact with BHK cells expressing HN but not F at their cell surface, human PBMC produced IFN-alpha and some cells, including monocytes and T lymphocytes, upregulated cell surface TRAIL expression...
  20. pmc Amino acid substitutions in the F-specific domain in the stalk of the newcastle disease virus HN protein modulate fusion and interfere with its interaction with the F protein
    Vanessa R Melanson
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655 0122, USA
    J Virol 78:13053-61. 2004
    ..In all cases, diminished fusion correlates with a decreased ability of the mutated HN protein to interact with F at the cell surface...
  21. ncbi Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity
    Angela Römer-Oberdörfer
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17493 Greifswald Insel Riems, Germany
    J Gen Virol 84:3121-9. 2003
    ..The HN protein, which is responsible for virus attachment to sialic acid-containing receptors, varies in length due to ..
  22. ncbi HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells
    P V Ravindra
    Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, India
    Arch Virol 153:749-54. 2008
    ..The results revealed that cells expressing the HN protein demonstrated decreased DNA content, phosphatidylserine exposure and increased cytoplasmic vacuolation...
  23. ncbi Identification of a variable epitope on the Newcastle disease virus hemagglutinin-neuraminidase protein
    Shunlin Hu
    Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, China
    Vet Microbiol 140:92-7. 2010
    ..of all NDV strains revealed that these MAb-resistant NDV isolates possessed residue K at position 347 of the HN protein, whereas all remaining strains possessed E or G at the same site...
  24. ncbi Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
    Olav S de Leeuw
    Wageningen University and Research Centre, Animal Sciences Group, Division of Infectious Diseases, 8200 AB Lelystad, The Netherlands
    J Gen Virol 86:1759-69. 2005
    ..The effect of the HN protein on virulence was most prominent after intravenous inoculation...
  25. ncbi Expression of a secreted version of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: its evaluation as a diagnostic reagent
    Silvina Chimeno Zoth
    Instituto de Biotecnologia, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnologia Agropecuaria, Castelar, Buenos Aires, Argentina
    J Vet Diagn Invest 23:519-23. 2011
    ..for baculovirus containing the sequence of a melittin signal peptide was constructed and the sequence coding for HN protein without its own signal peptide was cloned...
  26. ncbi Correlation of genetic variability with safety of mumps vaccine Urabe AM9 strain
    G Amexis
    Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852, USA
    Virology 287:234-41. 2001
    ..Determination of the mutational profile of mumps vaccine lots could allow vaccine manufacturers to characterize seed viruses and monitor the consistency of vaccine production to prevent emergence of virulent revertants...
  27. pmc The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence
    Zhuhui Huang
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 78:4176-84. 2004
    ..The tissue tropism of the viruses was shown to be dependent on the origin of the HN protein. The chimeric virus with the HN protein derived from the virulent virus exhibited a tissue predilection similar ..
  28. pmc Triggering of human parainfluenza virus 3 fusion protein (F) by the hemagglutinin-neuraminidase (HN) protein: an HN mutation diminishes the rate of F activation and fusion
    Matteo Porotto
    Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Virol 77:3647-54. 2003
    ..C28a HN is the first parainfluenza virus variant found so far to be specifically defective in HN's F-triggering and fusion promotion functions and may contribute to our understanding of transmission of the activating signal from HN to F...
  29. ncbi Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus
    C Madhan Mohan
    Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India
    J Virol Methods 138:117-22. 2006
    ..The assay proved to be sensitive, specific and accurate as compared to the standard haemagglutination inhibition (HI) test...
  30. pmc Premature activation of the paramyxovirus fusion protein before target cell attachment with corruption of the viral fusion machinery
    Shohreh F Farzan
    Department of Pediatrics, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 286:37945-54. 2011
    ..This first evidence that activation of a paramyxovirus F can be specifically induced before the virus contacts its target cell suggests a new strategy with broad implications for the design of antiviral agents...
  31. ncbi The ectodomains but not the transmembrane domains of the fusion proteins of subtypes A and B avian pneumovirus are conserved to a similar extent as those of human respiratory syncytial virus
    C J Naylor
    Institute for Animal Health, Compton Laboratory, Newbury, UK
    J Gen Virol 79:1393-8. 1998
    ..This may indicate that the two subtypes of APV have evolved in different geographical regions and/or different avian species. This is the first report of an F gene sequence from a subtype B APV...
  32. ncbi The anti-tumor effect of Newcastle disease virus HN protein is influenced by differential subcellular targeting
    Hong Sui
    Medical Department, The Tumor Hospital Affiliated Harbin Medical University, Harbin, Heilongjiang, China
    Cancer Immunol Immunother 59:989-99. 2010
    ..A DNA vaccine expressing the major immunogenic hemagglutinin-neuraminidase (HN) protein of this virus has shown promising results as an immunotherapeutic agent...
  33. pmc Interacting domains of the HN and F proteins of newcastle disease virus
    Kathryn A Gravel
    Program in Immunology and Virology, Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Virol 77:11040-9. 2003
    ..heptad repeat 2 (HR2) domain, we detected a specific interaction with amino acids 124 to 152 from the NDV HN protein. Biotin-labeled HR2 peptides bound to glutathione S-transferase (GST) fusion proteins containing these HN ..
  34. ncbi Cloning and expression of NDV hemagglutinin-neuraminidase cDNA in a baculovirus expression vector system
    E Nagy
    Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada
    Virology 176:426-38. 1990
    ..HN was copurified with extracellular virus (ECV) from the extracellular medium and was used to immunize chickens. The anti recHN serum was specific to NDV in both ELISA and Western blot analysis...
  35. ncbi [Anti-tumor immunity of Newcastle disease virus HN protein is influenced by differential subcellular targeting]
    Kaibing Wang
    Interventional Department, The Second Hospital Affiliated to Harbin Medical University, Harbin 150086, China
    Zhongguo Fei Ai Za Zhi 13:773-6. 2010
    ..We designed three different expression plasmids encoding the HN protein targeted to different subcellular compartments: cytoplasmic (Cy-HN), secreted (Sc-HN) and membrane-anchored (M-..
  36. ncbi [Induction of apoptosis in human hepatoma cell line SMMC7721 by Newcastle disease virus HN gene]
    Ying chun Sun
    Genetic Engineering Labotory of PLA, Academy of Military Medical Sciences, Changchun 130062, China
    Zhonghua Zhong Liu Za Zhi 27:279-82. 2005
    ..To investigate the mechanisms of apoptosis induced in human hepatoma cell line SMMC7721 by plasmid pVHN constructed with Newcastle disease virus (NDV) HN gene...
  37. ncbi Recombinant fowlpox viruses coexpressing chicken type I IFN and Newcastle disease virus HN and F genes: influence of IFN on protective efficacy and humoral responses of chickens following in ovo or post-hatch administration of recombinant viruses
    K Karaca
    Department of Pathobiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA
    Vaccine 16:1496-503. 1998
    ..Chickens vaccinated with FPV coexpressing IFN and NDV genes produced less antibodies against NDV in comparison with chickens vaccinated with FPV expressing NDV genes...
  38. pmc Fusion deficiency induced by mutations at the dimer interface in the Newcastle disease virus hemagglutinin-neuraminidase is due to a temperature-dependent defect in receptor binding
    Elizabeth A Corey
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
    J Virol 77:6913-22. 2003
    ..Instead, the interface mutations acted by weakening the interaction between HN and its receptor(s). The phenotype of these mutants correlates with the disruption of intermonomer subunit interactions...
  39. ncbi Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose
    Ping Yuan
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Structure 13:803-15. 2005
    ..The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells...
  40. ncbi Potentiation of cell-mediated immune responses against recombinant HN protein of Newcastle disease virus by recombinant chicken IL-18
    Bor Sheu Su
    Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan
    Vet Immunol Immunopathol 141:283-92. 2011
    In this study, recombinant fowlpox viruses (rFPV/HN) expressing Newcastle disease virus (NDV) HN protein and rFPV/HN/chIL-18 co-expressing chicken IL-18 (chIL-18) and HN protein have been constructed and characterized...
  41. ncbi Characterization of mumps virus strains with varying neurovirulence
    Farideh Rafiefard
    Department of Virology, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden
    Scand J Infect Dis 37:330-7. 2005
    ..Four of 5 C2 strains exhibited the amino acid arginine instead of lysine at position 335 of the HN protein, and the amino acid phenylalanine was found instead of serine at amino acid position 195 of the F protein...
  42. pmc Role of the two sialic acid binding sites on the newcastle disease virus HN protein in triggering the interaction with the F protein required for the promotion of fusion
    Paul J Mahon
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0002, USA
    J Virol 85:12079-82. 2011
    ....
  43. ncbi Single amino acid changes in the mumps virus haemagglutinin-neuraminidase and polymerase proteins are associated with neuroattenuation
    Tahir H Malik
    DVP Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    J Gen Virol 90:1741-7. 2009
    ..cDNA plasmid clones and site-directed mutagenesis, it was shown that the single amino acid change in the HN protein and to a lesser extent, the change in the L protein, resulted in neuroattenuation, as assessed in rats...
  44. ncbi Functional consequences of attenuating mutations in the haemagglutinin neuraminidase, fusion and polymerase proteins of a wild-type mumps virus strain
    Tahir Malik
    DVP Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    J Gen Virol 88:2533-41. 2007
    ..These data suggest a polygenic component (i.e. specific and inter-related roles of these amino acid changes) to MuV neuroattenuation...
  45. ncbi Characterization of naturally occurring parainfluenza virus type 2 (hPIV-2) variants
    O Terrier
    VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Universite Claude Bernard Lyon 1, Faculte de Medecine Laennec, 69372 Lyon Cedex 08, France
    J Clin Virol 43:86-92. 2008
    ..In most labs, the clinical diagnosis of hPIV is routinely done using techniques based on the detection of viral antigens such as immunofluorescence assay or/and viral isolation...
  46. ncbi Identification of regions on the fusion protein of human parainfluenza virus type 2 which are required for haemagglutinin-neuraminidase proteins to promote cell fusion
    M Tsurudome
    Department of Microbiology, Mie University School of Medicine, Tsu, Japan
    J Gen Virol 79:279-89. 1998
    ..human parainfluenza virus type 2 (PIV-2), while the PIV-2 F protein does not induce cell fusion with the SV-41 HN protein. In the present study, we found that the PIV-2 F protein induced extensive cell fusion with the HN protein of ..
  47. ncbi Presence of lysine at aa 335 of the hemagglutinin-neuraminidase protein of mumps virus vaccine strain Urabe AM9 is not a requirement for neurovirulence
    Christian J Sauder
    DVP Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Vaccine 27:5822-9. 2009
    ....
  48. pmc Generation of atypical pulmonary inflammatory responses in BALB/c mice after immunization with the native attachment (G) glycoprotein of respiratory syncytial virus
    G E Hancock
    Department of Immunology, Lederle Praxis Biologicals, Inc, West Henrietta, New York 14586 9728, USA
    J Virol 70:7783-91. 1996
    ..The results therefore suggest that G, not F, protein has more potential to bias the host for atypical pulmonary inflammatory responses...
  49. pmc A Y526Q mutation in the Newcastle disease virus HN protein reduces its functional activities and attenuates virus replication and pathogenicity
    Sunil K Khattar
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
    J Virol 83:7779-82. 2009
    ..C (BC), we mutated three conserved amino acids thought to be part of the binding/catalytic active site in the HN protein. We also mutated five additional residues near the proposed active site that are nonconserved between BC and the ..
  50. pmc Entry of parainfluenza virus into cells as a target for interrupting childhood respiratory disease
    Anne Moscona
    Department of Pediatrics, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Clin Invest 115:1688-98. 2005
    ..There are several steps during the process of binding, triggering, and fusion that are now understood at the molecular level, and each of these steps represents potential targets for interrupting infection...
  51. pmc Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion
    Viatcheslav Zaitsev
    Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, United Kingdom
    J Virol 78:3733-41. 2004
    ..Three different crystal forms of NDV HN now reveal identical tetrameric arrangements of HN monomers, perhaps indicative of the tetramer association found on the viral surface...
  52. pmc Influence of the human parainfluenza virus 3 attachment protein's neuraminidase activity on its capacity to activate the fusion protein
    Matteo Porotto
    Department of Pediatrics, Mount Sinai School of Medicine, 1 Gustave L Levy Pl, New York, NY 10029, USA
    J Virol 79:2383-92. 2005
    ..Any particular HN's competence to promote F-mediated fusion depends on the balance between its inherent F-triggering efficacy and its receptor-attachment regulatory functions (binding and receptor cleavage)...
  53. pmc N-linked glycan at residue 523 of human parainfluenza virus type 3 hemagglutinin-neuraminidase masks a second receptor-binding site
    Vasiliy P Mishin
    Department of Infectious Diseases, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105 3678, USA
    J Virol 84:3094-100. 2010
    ..Our data are consistent with the idea that, similar to the case for hPIV-1, the N-linked glycan shields a second receptor-binding site on hPIV-3 HN...
  54. pmc Spring-loaded model revisited: paramyxovirus fusion requires engagement of a receptor binding protein beyond initial triggering of the fusion protein
    Matteo Porotto
    Departments of Pediatrics and of Microbiology and Immunology, Weill Medical College of Cornell University, 515 East 71st Street, 6th Floor, Box 309, New York, NY 10021, USA
    J Virol 85:12867-80. 2011
    ..This model has broad implications for the mechanism of paramyxovirus fusion and for strategies to prevent viral entry...
  55. pmc Human parainfluenza virus infection of the airway epithelium: viral hemagglutinin-neuraminidase regulates fusion protein activation and modulates infectivity
    Laura M Palermo
    Department of Pediatrics and of Microbiology and Immunology, Weill Medical College of Cornell University, 515 East 71st Street, 6th Floor, New York, NY 10021, USA
    J Virol 83:6900-8. 2009
    ..The finding that the dysregulation of F triggering prohibits successful infection in HAE cells suggests that antiviral strategies targeted to HN's F-triggering activity may have promise in vivo...
  56. ncbi Structure-function analysis of two variants of mumps virus hemagglutinin-neuraminidase protein
    Gerardo Santos-López
    Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Metepec, Puebla, Mexico
    Braz J Infect Dis 13:24-34. 2009
    ..the present study, we performed a structure-function analysis of that amino acid substitution; the structures of HN protein of both Urabe AM9 strain variants were predicted...
  57. ncbi Role of sialic acid-containing molecules in paramyxovirus entry into the host cell: a minireview
    Enrique Villar
    Departamento de Bioquimica y Biologia Molecular, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s n, Lab 108, Salamanca 37007, Spain
    Glycoconj J 23:5-17. 2006
    ..In spite of all these data, the exact nature of paramyxovirus receptors, apart from their sialylated nature, and the mechanism(s) of viral attachment to the cell surface are poorly understood...
  58. pmc Detailed genetic analysis of hemagglutinin-neuraminidase glycoprotein gene in human parainfluenza virus type 1 isolates from patients with acute respiratory infection between 2002 and 2009 in Yamagata prefecture, Japan
    Katsumi Mizuta
    Yamagata Prefectural Institute of Public Health, 1 6 6 Toka machi, Yamagata shi, Yamagata 990 0031, Japan
    Virol J 8:533. 2011
    ....
  59. ncbi Amino acid change 335 E to K affects the sialic-acid-binding and neuraminidase activities of Urabe AM9 mumps virus hemagglutinin-neuraminidase glycoprotein
    Julio Reyes-Leyva
    Laboratorio de Virología y Biología Molecular, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Mexico
    Microbes Infect 9:234-40. 2007
    ..Therefore, HN-K335 variant presented a neuraminidase activity level 11-fold higher than that of HN-E335 variant. In conclusion, the mutation affects the receptor-binding and neuraminidase activities of Urabe AM9 mumps virus variants...
  60. ncbi Phylogenetic characterization of Newcastle disease virus isolated in the mainland of China during 2001-2009
    Zhang Rui
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China
    Vet Microbiol 141:246-57. 2010
    ..The results indicate that recombination play a role in NDV evolution and the live vaccines have capacity to boost NDV evolution by homologous recombination with circulating virus...
  61. pmc Domain architecture and oligomerization properties of the paramyxovirus PIV 5 hemagglutinin-neuraminidase (HN) protein
    Ping Yuan
    Department of Structural Biology, Stanford University, Palo Alto, CA 94305 5126, USA
    Virology 378:282-91. 2008
    ..Electron microscopy of the HN ectodomain reveals flexible arrangements of the NA and stalk domains, which may be important for understanding how these two HN domains impact virus entry...
  62. ncbi Structure of the haemagglutinin-neuraminidase from human parainfluenza virus type III
    Michael C Lawrence
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville, VIC 3052, Australia
    J Mol Biol 335:1343-57. 2004
    ..However, in contrast to the NDV structures, we observe no ligand-induced structural changes that extend beyond the active site and modify the dimer interface...
  63. ncbi Evaluation of Newcastle disease virus chimeras expressing the Hemagglutinin-Neuraminidase protein of velogenic strains in the context of a mesogenic recombinant virus backbone
    Carlos Estevez
    Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA
    Virus Res 129:182-90. 2007
    ..A HN gene exchange alone within the context of the NDV Anhinga backbone failed to increase virus virulence from mesogenic to velogenic pathotype and suggests a multigenic role for NDV pathogenicity...
  64. pmc A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein
    Emma Herrera
    Lab Virología, ENCB IPN Carpio y Plan de Ayala S N Casco de Santo Tomás, México D, F, 11340 Mexico
    Virol J 7:195. 2010
    ....
  65. pmc Variation of a newcastle disease virus hemagglutinin-neuraminidase linear epitope
    Sun Hee Cho
    Zoonotic Disease Institute, Seoul National University, San 56 1, Shillim Dong, Gwanak Gu, Seoul 151 742, South Korea
    J Clin Microbiol 46:1541-4. 2008
    ..The antigenicities of the variants that became predominant in Korea differ from each other and from the wild type...
  66. ncbi Sialidase, receptor-binding and fusion-promotion activities of Newcastle disease virus haemagglutinin-neuraminidase glycoprotein: a mutational and kinetic study
    Laura Ferreira
    Departamento de Bioquimica y Biologia Molecular, Universidad de Salamanca, Plaza Doctores de la Reina s n, 37007 Salamanca, Spain
    J Gen Virol 85:1981-8. 2004
    ..These results also suggest that the globular head of HN protein may play a role in fusion-promotion activity.
  67. pmc Virus-specific memory and effector T lymphocytes exhibit different cytokine responses to antigens during experimental murine respiratory syncytial virus infection
    A Srikiatkhachorn
    Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville 22908, USA
    J Virol 71:678-85. 1997
    ....
  68. pmc Loss of the N-linked glycan at residue 173 of human parainfluenza virus type 1 hemagglutinin-neuraminidase exposes a second receptor-binding site
    Irina V Alymova
    Department of Infectious Diseases, Mail Stop 330, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, Tennessee 38105 2794, USA
    J Virol 82:8400-10. 2008
    ..Our study suggests that the N-linked glycan at residue 173 masks a second receptor-binding site on hPIV-1 HN...
  69. pmc A second receptor binding site on human parainfluenza virus type 3 hemagglutinin-neuraminidase contributes to activation of the fusion mechanism
    Matteo Porotto
    Department of Pediatrics, Weill Medical College of Cornell University, 515 East 71st Street, New York, NY 10021, USA
    J Virol 81:3216-28. 2007
    ....
  70. pmc Mutation at residue 523 creates a second receptor binding site on human parainfluenza virus type 1 hemagglutinin-neuraminidase protein
    Tatiana Bousse
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Virol 80:9009-16. 2006
    ..Our study indicates that the presence and requirement of a second binding site vary among paramyxoviruses...
  71. pmc Biological significance of the second receptor binding site of Newcastle disease virus hemagglutinin-neuraminidase protein
    Tatiana L Bousse
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Virol 78:13351-5. 2004
    ..Analysis of the three-dimensional structure of Newcastle disease virus (NDV) HN protein revealed the presence of a large pocket, which mediates both receptor binding and NA activities...
  72. pmc Probing the sialic acid binding site of the hemagglutinin-neuraminidase of Newcastle disease virus: identification of key amino acids involved in cell binding, catalysis, and fusion
    Helen Connaris
    Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, Scotland
    J Virol 76:1816-24. 2002
    ..These results further support the single-site model and suggest certain residues to be important for the triggering of fusion...
  73. ncbi Hemagglutinin-neuraminidase sequence and phylogenetic analyses of mumps virus isolates from a vaccinated population in Singapore
    C S Lim
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore
    J Med Virol 70:287-92. 2003
    ..Other significant amino acid disparities (e.g., at residue 354) may also affect the antigenic properties of the HN protein. These findings suggest that the evolution and adaptation of the currently circulating mumps virus strains in ..
  74. ncbi Molecular epidemiology of mumps virus in Japan and proposal of two new genotypes
    Yoko Inou
    Department of Pediatrics, Tokyo Medical College, Nishishinjuku, Shinjuku ku, Tokyo, Japan
    J Med Virol 73:97-104. 2004
    ..We should continue to watch on mumps virus molecular epidemiology...
  75. pmc Structural and functional relationship between the receptor recognition and neuraminidase activities of the Newcastle disease virus hemagglutinin-neuraminidase protein: receptor recognition is dependent on neuraminidase activity
    R M Iorio
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
    J Virol 75:1918-27. 2001
    ..analysis of the role of these residues in the functional activity of the Newcastle disease virus (NDV) HN protein. Substitutions for several of these residues result in a protein lacking both detectable NA and receptor ..
  76. pmc Inhibition of parainfluenza virus type 3 and Newcastle disease virus hemagglutinin-neuraminidase receptor binding: effect of receptor avidity and steric hindrance at the inhibitor binding sites
    Matteo Porotto
    Department of Pediatrics, Mount Sinai School of Medicine, 1 Gustave L Levy Pl, New York, NY 10029, USA
    J Virol 78:13911-9. 2004
    ....
  77. ncbi Characterization of genotype-specific epitopes of the HN protein of mumps virus
    C Orvell
    Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Sweden
    J Gen Virol 78:3187-93. 1997
    ..activity in mice and rabbits was not corroborated by an overall difference in the amino acid sequence of the HN protein of the different genotypes...
  78. pmc Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated by human parainfluenza type 2 virus
    Masato Tsurudome
    Department of Microbiology, Mie University Graduate School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507 Japan
    J Virol 82:8283-95. 2008
    ..is a neutralization escape variant selected with M1-1A and contains amino acid mutations N83Y and M186I in the HN protein, with no mutation in the fusion protein...
  79. ncbi Genetic diversity of mumps virus in oral fluid specimens: application to mumps epidemiological study
    Li Jin
    Enteric, Respiratory, and Neurological Virus Laboratory, Communicable Disease Surveillance Center, Health Protection Agency, London, United Kingdom
    J Infect Dis 189:1001-8. 2004
    ....
  80. pmc Hemagglutinin-neuraminidase-independent fusion activity of simian virus 5 fusion (F) protein: difference in conformation between fusogenic and nonfusogenic F proteins on the cell surface
    M Tsurudome
    Department of Microbiology, Mie University School of Medicine, Tsu, Mie 514 8507, Japan
    J Virol 75:8999-9009. 2001
    ..In contrast, the F protein of SV5 strain WR induces cell fusion only when coexpressed with the HN protein, the same as do other paramyxovirus F proteins...
  81. ncbi Newcastle disease virus fusion and haemagglutinin-neuraminidase gene motifs as markers for viral lineage
    A R Gould
    CSIRO Livestock Industries, Australian Animal Health Laboratory, P O Bag 24, Geelong, Victoria 3220, Australia
    Avian Pathol 32:361-73. 2003
    ..A proposed mechanism for this observation is discussed...
  82. ncbi Effect of host selective pressure on Newcastle disease virus virulence
    Flavia Zanetti
    Instituto de Biotecnologia, Centro de Investigaciones en Ciencias Veterinarias y Agronomicas, Instituto Nacional de Tecnologia Agropecuaria, Las Cabañas y Los Reseros s n, Hurlingham, Buenos Aires, Argentina
    Microb Pathog 44:135-40. 2008
    ..Nucleotide changes in the F protein cleavage site amino acid sequence and in the hemagglutinin-neuraminidase protein sequence are reported...
  83. pmc The large polymerase protein is associated with the virulence of Newcastle disease virus
    Subrat N Rout
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA
    J Virol 82:7828-36. 2008
    ..Our findings offer new insights into the pathogenesis of NDV infection...
  84. ncbi The pathogenesis of Newcastle disease: a comparison of selected Newcastle disease virus wild-type strains and their infectious clones
    Nobuko Wakamatsu
    Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, GA 30605, USA
    Virology 353:333-43. 2006
    ..Both pathogenicity indices and clinicopathologic examination demonstrated that the F, HN, and P genes of NDV collectively or individually can contribute to viral virulence...
  85. ncbi Avian adeno-associated virus-based expression of Newcastle disease virus hemagglutinin-neuraminidase protein for poultry vaccination
    F Perozo
    Poultry Diagnostic and Research Center, University of Georgia, Athens, GA 30602, USA
    Avian Dis 52:253-9. 2008
    ..In this work the generation of recombinant AAAV virions expressing the HN protein (rAAAV-HN) was demonstrated by electron microscopy, immunocytochemistry, and western blot analysis...
  86. ncbi Effects of heptad repeat regions of f protein on the specific membrane fusion in paramyxoviruses
    Guijie Ren
    Department of Virology, School of Public Health, Shandong University, Jinan, People s Republic of China
    Intervirology 49:299-306. 2006
    ..To identify the effects of heptad repeat regions (HR1 and HR2) of F on the specific membrane fusion in paramyxoviruses...
  87. pmc Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein
    Surbhi Jain
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 81:2328-39. 2007
    ..cell-cell fusion and virus entry but had no effect on cell viability, glycoprotein surface expression, or HN protein attachment or neuraminidase activities...
  88. ncbi Deduced amino acid sequences surrounding the fusion glycoprotein cleavage site and of the carboxyl-terminus of haemagglutinin-neuraminidase protein of the avirulent thermostable vaccine strain I-2 of Newcastle disease virus
    P N Wambura
    School of Veterinary Science, University of Queensland, Brisbane, Australia
    Vet Res Commun 31:105-12. 2007
    ..analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains...
  89. ncbi Protection of chickens from Newcastle disease and infectious laryngotracheitis with a recombinant fowlpox virus co-expressing the F, HN genes of Newcastle disease virus and gB gene of infectious laryngotracheitis virus
    Hui ling Sun
    China Agriculture University, Beijing 100094, People s Republic of China
    Avian Dis 52:111-7. 2008
    ..The protective efficacy was also measured by the antibody responses and isolation of challenge viruses. Results showed that this rFPV could be a potential vaccine for preventing NDV and ILTV by a single immunization...
  90. pmc Paramyxoviruses: different receptors - different mechanisms of fusion
    Ronald M Iorio
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Trends Microbiol 16:135-7. 2008
    ....
  91. ncbi Synergistic effects of adjuvants interferon-gamma and levamisole on DNA vaccination against infection with Newcastle disease virus
    Jiangmei Yin
    State Key Laboratory for Agro Biotechnology, Key Laboratory of Agro Microbial Resource, and Application of MOA, College of Biology, China Agricultural University, Beijing, China
    Viral Immunol 20:288-99. 2007
    ..This study demonstrates that the synergistic effects of provax-chIFN-gamma plus LMS as the adjuvants in NDV DNA vaccination could be used to improve protective efficacy in chickens...
  92. pmc Incorporation of functional HN-F glycoprotein-containing complexes into newcastle disease virus is dependent on cholesterol and membrane lipid raft integrity
    Jason P Laliberte
    Department of Molecular Genetics and Microbiology, Room S5 250, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 81:10636-48. 2007
    ....
  93. ncbi Over-expression of mammalian sialidase NEU3 reduces Newcastle disease virus entry and propagation in COS7 cells
    Luigi Anastasia
    Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Milan, Italy
    Biochim Biophys Acta 1780:504-12. 2008
    ..Evidence was also provided for the preferential association of NDV-HN at 4 degrees C to detergent resistant microdomains (DRMs) of COS7 cells plasma membranes...
  94. pmc Molecular characterization of three new virulent Newcastle disease virus variants isolated in China
    Lei Tao Tan
    Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250100, China
    J Clin Microbiol 46:750-3. 2008
    ..In comparison with the LaSota live virus vaccine, these viruses had significant genetic variations in the hemagglutinin-neuraminidase gene...
  95. ncbi Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs
    C M Fuller
    Virology Department, Veterinary Laboratories Agency, Addlestone, Surrey, UK
    Arch Virol 152:1575-82. 2007
    ..49, T --> C at position 1769 in the untranslated region of the fusion gene and G --> A at position 437 of the HN gene, resulting in the amino acid change G --> R at position 116 in the HN protein.
  96. ncbi Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins
    A Rabu
    Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
    Acta Virol 46:211-7. 2002
    ..Chickens inoculated with the chimeric particles mounted an immune response against NDV, suggesting the possibility of use of the ring-like particle as a carrier of immunogens in subunit vaccines and immunological reagents...
  97. pmc Mucosal immunization of rhesus monkeys against respiratory syncytial virus subgroups A and B and human parainfluenza virus type 3 by using a live cDNA-derived vaccine based on a host range-attenuated bovine parainfluenza virus type 3 vector backbone
    Alexander C Schmidt
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 76:1089-99. 2002
    ....
  98. ncbi Improved protection from velogenic Newcastle disease virus challenge following multiple immunizations with plasmid DNA encoding for F and HN genes
    C F Loke
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Vet Immunol Immunopathol 106:259-67. 2005
    ..The results suggested that more than two injections with both F and HN encoding plasmid DNA were required to induce higher level of antibodies for protection against velogenic NDV in chickens...
  99. pmc Decreased dependence on receptor recognition for the fusion promotion activity of L289A-mutated newcastle disease virus fusion protein correlates with a monoclonal antibody-detected conformational change
    Jianrong Li
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave No, Worcester, MA 01655 0122, USA
    J Virol 79:1180-90. 2005
    ..L289A-F protein exhibits the same specificity as the wt F protein for the homologous HN protein, as well as NDV-human parainfluenza virus 3 HN chimeras...
  100. ncbi High cell surface expression of Newcastle disease virus proteins via replicon vectors demonstrates syncytia forming activity of F and fusion promotion activity of HN molecules
    Jinyang Zeng
    Division of Cellular Immunology, German Cancer Research Center, D010, D 69129 Heidelberg, Germany
    Int J Oncol 25:293-302. 2004
    ..F alone, expressed via this vector, displayed residual fusion activity suggesting its proteolytic cleavage and its functional independence from HN...
  101. pmc Mutated form of the Newcastle disease virus hemagglutinin-neuraminidase interacts with the homologous fusion protein despite deficiencies in both receptor recognition and fusion promotion
    Jianrong Li
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655 0122, USA
    J Virol 78:5299-310. 2004
    ..Both the attachment and fusion-promoting activities of this mutant HN protein can be rescued either by NA activity contributed by another HN protein or by a set of four substitutions at the ..

Research Grants5

  1. STRUCTURE AND EXPRESSION OF THE PARAMYXOVIRUS SV5 GENOME
    Robert A Lamb; Fiscal Year: 2010
    ..We will elucidate the role of mutations in the F protein in the context of a virus infection by using reverse genetics and we will study fusion activity, virus growth and virus assembly of the altered viruses. ..
  2. PARAMYXOVIRUS MEMBRANE FUSION
    TRUDY MORRISON; Fiscal Year: 2005
    ..Our studies have focused on how F protein mediates membrane fusion and the role of HN protein in that process...
  3. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2008
    ..abstract_text> ..
  4. Design of peptide entry inhibitors and delivery systems to target emerging henipa
    Anne Moscona; Fiscal Year: 2010
    ..abstract_text> ..