Genomes and Genes
Summary: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Publications317 found, 100 shown here
- Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesisChristiana Ruhrberg
Endothelial Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, United Kingdom
Genes Dev 16:2684-98. 2002..We found that VEGF(120/120) mouse embryos, engineered to express solely an isoform of VEGF-A that lacks heparin-binding, and therefore extracellular matrix interaction domains, exhibited a specific decrease in capillary ..
- Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfateY Chen
Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
Nat Med 3:866-71. 1997..b>Heparin, highly sulfated heparan sulfate, and the polysulfonate pharmaceutical Suramin effectively prevented dengue virus ..
- A heparin-binding growth factor secreted by macrophage-like cells that is related to EGFS Higashiyama
Department of Surgical Research, Children s Hospital, Boston, MA 02115
Science 251:936-9. 1991Macrophage-like U-937 cells secrete a 22-kilodalton heparin-binding growth factor that is mitogenic for BALB-3T3 fibroblasts and smooth muscle cells, but not endothelial cells...
- Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cellsN Ferrara
Department of Pharmacological Sciences, Genentech Inc, South San Francisco, CA 94080
Biochem Biophys Res Commun 161:851-8. 1989..bovine pituitary follicular cells and purified to homogeneity by a combination of ammonium sulfate precipitation, heparin-sepharose affinity chromatography and two reversed phase HPLC steps...
- Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine ciliaAnia T Deutscher
Industry and Investment NSW, Elizabeth Macarthur Agricultural Institute, NSW, Australia
Mol Microbiol 78:444-58. 2010..Eight pentapeptide repeats in R1 are sufficient to bind porcine cilia; however, both R1 and R2 are needed to bind heparin. Mhp271, a paralogue of P97, is the only other M. hyopneumoniae protein to contain both R1 and R2 repeats...
- P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cellsTracey A Burnett
NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
Mol Microbiol 60:669-86. 2006..b>Heparin is known to inhibit adherence of M. hyopneumoniae to porcine respiratory epithelial cilia. M...
- Human papillomavirus infection requires cell surface heparan sulfateT Giroglou
Institute for Medical Microbiology and Hygiene, University of Mainz, 55101 Mainz, Germany
J Virol 75:1565-70. 2001..Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by ..
- Structure of a Plasmodium falciparum PfEMP1 rosetting domain reveals a role for the N-terminal segment in heparin-mediated rosette inhibitionAlexandre Juillerat
Institut Pasteur, Unité d Immunologie Structurale, 75015 Paris, France
Proc Natl Acad Sci U S A 108:5243-8. 2011..b>Heparin and other sulfated oligosaccharides, however, can disrupt rosettes, suggesting that therapeutic approaches to ..
- Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experimentYing Zheng Zhao
Wenzhou Medical College, Wenzhou City, Zhejiang Province, China
J Drug Target 20:623-31. 2012..factor (aFGF) intravenous delivery to the ischemic myocardium of rats by ultrasonic microbubbles modified with heparin. Heparin modified microbubbles (HMB) were prepared by the freeze-dried method...
- Alboserpin, a factor Xa inhibitor from the mosquito vector of yellow fever, binds heparin and membrane phospholipids and exhibits antithrombotic activityEric Calvo
Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
J Biol Chem 286:27998-8010. 2011..Alboserpin displays high affinity binding to heparin (K(D) ~ 20 nM), but no change in FXa inhibition was observed in the presence of the cofactor, implying that ..
- Treatment and prevention of heparin-induced thrombocytopenia: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice GuidelinesLori Ann Linkins
Department of Medicine, McMaster University, Juravinski Hospital, Rm M0118, 1280 Main St W, Hamilton, ON, L8S 4K1, Canada
Chest 141:e495S-530S. 2012b>Heparin-induced thrombocytopenia (HIT) is an antibody-mediated adverse drug reaction that can lead to devastating thromboembolic complications, including pulmonary embolism, ischemic limb necrosis necessitating limb amputation, acute ..
- Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferationAkira Sonoda
Section of Pediatric Dentistry, Division of Oral Health, Growth and Development, Kyushu University, Fukuoka 812 8582, Japan
J Biol Chem 284:27176-84. 2009..By using recombinant AMBN proteins, we found that AMBN had heparin binding domains at the C-terminal half and that these domains were critical for AMBN binding to dental epithelial ..
- Mhp493 (P216) is a proteolytically processed, cilium and heparin binding protein of Mycoplasma hyopneumoniaeJody Wilton
NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
Mol Microbiol 71:566-82. 2009..b>Heparin binds to M. hyopneumoniae cells in a dose-dependent manner and blocks its ability to adhere to porcine cilia...
- Mast cells increase vascular permeability by heparin-initiated bradykinin formation in vivoChris Oschatz
Clinical Chemistry, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
Immunity 34:258-68. 2011..We report a paracrine mechanism by which mast cell-released heparin increases vascular permeability in vivo...
- Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypesZhijian Wu
Gene Therapy Center, 7113 Thurston Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7352, USA
J Virol 80:11393-7. 2006..acid residues that differ between AAV1 and AAV6 revealed that a lysine-to-glutamate change (K531E) suppresses the heparin binding ability of AAV6...
- Two domains within the Mycoplasma hyopneumoniae cilium adhesin bind heparinCheryl Jenkins
Microbiology and Immunology, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden, New South Wales 2570, Australia
Infect Immun 74:481-7. 2006..hyopneumoniae is lacking. However, heparin and other sulfated polysaccharides are known to block the binding of M...
- Specific molecular interactions of oversulfated chondroitin sulfate E with various heparin-binding growth factors. Implications as a physiological binding partner in the brain and other tissuesSarama Sathyaseelan Deepa
Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Japan
J Biol Chem 277:43707-16. 2002We previously observed that the cortical neuronal cell adhesion mediated by midkine (MK), a heparin (Hep)-binding growth factor, is specifically inhibited by oversulfated chondroitin sulfate-E (CS-E) (Ueoka, C., Kaneda, N., Okazaki, I...
- FGFs, heparan sulfate and FGFRs: complex interactions essential for developmentD M Ornitz
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
Bioessays 22:108-12. 2000..All FGFs have a high affinity for the glycosaminoglycan heparin and for cell surface heparan sulfate proteoglycans...
- Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST): novel characteristics of NDST-1 and -2Jacob van den Born
Division of Nephrology, University Hospital St Radboud, PO Box 9101, 6500 HB, Nijmegen, The Netherlands
Glycobiology 13:1-10. 2003..N-deacetylase/N-sulfotransferases (NDSTs), which are key enzymes in sulfation of heparan sulfate (HS)/heparin. The assay is based on the recognition of NDST-generated N-unsubstituted glucosamine units in Escherichia coli K5 ..
- Caring for the critically ill patient. High-dose antithrombin III in severe sepsis: a randomized controlled trialB L Warren
Brown University School of Medicine, Infectious Disease Division, Memorial Hospital of Rhode Island, 111 Brewster St, Pawtucket, RI 02860, USA
JAMA 286:1869-78. 2001..In the subgroup of patients who did not receive concomitant heparin during the 4-day treatment phase (n = 698), the 28-day mortality was nonsignificantly lower in the antithrombin ..
- Passage of dengue virus type 4 vaccine candidates in fetal rhesus lung cells selects heparin-sensitive variants that result in loss of infectivity and immunogenicity in rhesus macaquesGermán Añez
Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8005, USA
J Virol 83:10384-94. 2009..in FRhL cells showed increased affinity for heparan sulfate compared to the unpassaged DENV-4, as measured by heparin binding and infectivity inhibition assays...
- Heparin impairs angiogenesis through inhibition of microRNA-10bXiaokun Shen
Glycochemistry and Glycobiology Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai
J Biol Chem 286:26616-27. 2011b>Heparin, which has been used as an anticoagulant drug for decades, inhibits angiogenesis, whereas thrombin promotes tumor-associated angiogenesis...
- Heparin increases the infectivity of Human Papillomavirus type 16 independent of cell surface proteoglycans and induces L1 epitope exposureCarla Cerqueira
Emmy Noether Group Virus Endocytosis, Institutes of Molecular Virology and Medical Biochemistry, University of Munster, Munster, Germany
Cell Microbiol 15:1818-36. 2013..Here, we analyse the phenomenon that preincubation of HPV-16 with increasing concentrations of heparin results in partial restoration rather than more efficient inhibition of infection...
- Unfractionated heparin and new heparin analogues from ascidians (chordate-tunicate) ameliorate colitis in ratsCelso L R Belmiro
Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho HUCFF and Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro UFRJ, Rio de Janeiro 21941 913, Brazil
J Biol Chem 284:11267-78. 2009The anti-inflammatory effect of mammalian heparin analogues, named dermatan sulfate and heparin, isolated from the ascidian Styela plicata was accessed in a TNBS-induced colitis model in rats...
- N-arginine dibasic convertase is a specific receptor for heparin-binding EGF-like growth factor that mediates cell migrationE Nishi
Department of Surgical Research, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
EMBO J 20:3342-50. 2001b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a mitogen and chemotactic factor, binds to two receptor tyrosine kinases, erbB1 and erbB4...
- Regional citrate versus heparin anticoagulation for continuous renal replacement therapy: a meta-analysis of randomized controlled trialsMei Yi Wu
Division of Nephrology, Department of Internal Medicine, Taipei Medical University Shuang Ho Hospital, Taipei, Taiwan
Am J Kidney Dis 59:810-8. 2012Anticoagulation of the extracorporeal circuit is required in continuous renal replacement therapy (CRRT). Heparin is the classic choice for anticoagulation, although it may increase the risk of bleeding...
- Heparin enhances the cell-protein misfolding cyclic amplification efficiency of variant Creutzfeldt-Jakob diseaseTakashi Yokoyama
Research and Development Division, Benesis Corporation, c o Division of Neurological Science, Department of Prion Research, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai, Japan
Neurosci Lett 498:119-23. 2011..PrP(res) amounts after cell-PMCA were analyzed by western blotting. Heparin, dermatan sulfate, and dextran sulfate (average molecular weight [MW] 1400kDa) enhanced efficiency, but dextran ..
- Characterization of heparin-binding site of tissue transglutaminase: its importance in cell surface targeting, matrix deposition, and cell signalingZhuo Wang
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, United Kingdom
J Biol Chem 287:13063-83. 2012..A novel regulatory mechanism for TG2 translocation into the extracellular compartment that depends upon TG2 conformation and the binding of HS is proposed...
- NMR structural determinants of eosinophil cationic protein binding to membrane and heparin mimeticsMaría Flor García-Mayoral
Instituto de Quimica Fisica Rocasolano, CSIC, Madrid, Spain
Biophys J 98:2702-11. 2010..We have used NMR spectroscopy to characterize the binding of ECP to membrane and heparin mimetics at a residue level...
- Heparan sulfate/heparin oligosaccharides protect stromal cell-derived factor-1 (SDF-1)/CXCL12 against proteolysis induced by CD26/dipeptidyl peptidase IVRabia Sadir
Institut de Biologie Structurale, CNRS Commissariat à l Enérgie Atomique Université Joseph Fourier, Unité Mixte de Recherche 5075, 41 Rue Horowitz, 38027 Grenoble Cedex 01, France
J Biol Chem 279:43854-60. 2004..We report here that heparin and HS specifically prevent the processing of SDF-1 by DPP IV expressed by Caco-2 cells...
- Self-assembling nanocomplexes by combining ferumoxytol, heparin and protamine for cell tracking by magnetic resonance imagingMya S Thu
Frank Laboratory and Laboratory of Diagnostic Radiology Research, Department of Radiology and Imaging Sciences, US National Institutes of Health, Bethesda, Maryland, USA
Nat Med 18:463-7. 2012..cell-labeling approach that combines three US Food and Drug Administration (FDA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes that effectively label cells for in ..
- The potential role of heparin in assisted conceptionScott M Nelson
Reproductive and Maternal Medicine, Division of Developmental Medicine, University of Glasgow, Glasgow Royal Infirmary, 10 Alexandra Parade, Glasgow G31 ER, UK
Hum Reprod Update 14:623-45. 2008Heparan sulphates play key roles in conception and early pregnancy events. The role of heparin, a structural analogue, and its application to assisted conception, is largely unknown.
- A systems biology approach for the investigation of the heparin/heparan sulfate interactomeAlessandro Ori
Institute of Integrative Biology and Centre for Glycobiology, University of Liverpool, Liverpool, United Kingdom
J Biol Chem 286:19892-904. 2011..We collected 435 human proteins interacting with HS or the structurally related heparin by integrating literature-derived and affinity proteomics data...
- Characterization of the chemokine CXCL11-heparin interaction suggests two different affinities for glycosaminoglycansIndia C Severin
Merck Serono Geneva Research Centre, 9 Chemin des Mines, 1202 Geneva, Switzerland
J Biol Chem 285:17713-24. 2010..residues in the C-terminal helix (the 50s cluster, (57)KSKQAR(62)) along with Lys(17), significantly impaired heparin binding in vitro, identifying these residues as components of the dominant epitope...
- The Mycoplasma gallisepticum OsmC-like protein MG1142 resides on the cell surface and binds heparinCheryl Jenkins
Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia
Microbiology 153:1455-63. 2007..In this study, M. gallisepticum whole cells were shown to bind heparin in a specific and saturable manner. Heparin also significantly inhibited the binding of M...
- Antithrombin-mediated anticoagulant activity of sulfated polysaccharides: different mechanisms for heparin and sulfated galactansFabio R Melo
Laboratório de Tecido Conjuntivo, Hospital Universitario Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ 21941 590, Brazil
J Biol Chem 279:20824-35. 2004..3) Sulfated galactans require significantly longer chains than heparin to achieve anticoagulant activity...
- Stability of PEI-DNA and DOTAP-DNA complexes: effect of alkaline pH, heparin and serumI Moret
, Facultad de Medicina, Universitat de Valencia, , Valencia, Spain
J Control Release 76:169-81. 2001..3 to 13.4) or by adding heparin. The stability against degradation was tested in vitro, by incubating the complexes at 37 degrees C in the ..
- Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytesK Hashimoto
Department of Dermatology, Osaka University Medical School, Japan
J Biol Chem 269:20060-6. 1994Since heparin-binding epidermal growth factor (HB-EGF) is a member of the EGF family and binds to EGF receptor, we tested recombinant HB-EGF for its ability to stimulate human keratinocyte proliferation...
- Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infectionL K Hallak
Immunology Microbiology, Biochemistry and Pathology, Rush Presbyterian St Luke s Medical Center, Chicago, IL 60612, USA
Virology 271:264-75. 2000..experiments in which RSV was preincubated with various soluble GAGs revealed the relative blocking order of: heparin > heparan sulfate > chondroitin sulfate B. Iduronic acid is a component common to these GAGs...
- Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosisJean Michel Hougardy
Laboratory of Vaccinology and Mucosal Immunity, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
PLoS ONE 2:e926. 2007..We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI.
- Heparin stimulates Staphylococcus aureus biofilm formationRobert M Q Shanks
Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
Infect Immun 73:4596-606. 2005b>Heparin, known for its anticoagulant activity, is commonly used in catheter locks...
- Induction and expression of heparin-binding EGF-like growth factor in human pancreatic cancerM S Kobrin
Department of Medicine, University of California, Irvine 92717
Biochem Biophys Res Commun 202:1705-9. 1994b>Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor...
- Regulation of Wnt signaling and embryo patterning by an extracellular sulfataseG K Dhoot
Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
Science 293:1663-6. 2001....
- Mutations in factor H reduce binding affinity to C3b and heparin and surface attachment to endothelial cells in hemolytic uremic syndromeTamara Manuelian
Hans Knoell Institute for Natural Products Research, Beutenbergstrasse 11a, D 07745 Jena, Germany
J Clin Invest 111:1181-90. 2003..All three mutations cause reduced binding to the central complement component C3b/C3d to heparin, as well as to endothelial cells...
- Regional citrate versus systemic heparin anticoagulation for continuous renal replacement in critically ill patientsDemetrios J Kutsogiannis
Division of Critical Care Medicine, Department of Public Health Sciences, The University of Alberta, Edmonton, Alberta, Canada
Kidney Int 67:2361-7. 2005We determined the effect of regional citrate versus systemic heparin anticoagulation for continuous renal replacement therapy in critically ill subjects suffering from acute renal failure who were not at high risk for hemorrhagic ..
- Heparin plus a glycoprotein IIb/IIIa inhibitor versus bivalirudin monotherapy and paclitaxel-eluting stents versus bare-metal stents in acute myocardial infarction (HORIZONS-AMI): final 3-year results from a multicentre, randomised controlled trialGregg W Stone
New York Presbyterian Hospital, Columbia University Medical Center, New York, NY 10022, USA
Lancet 377:2193-204. 2011..Primary results of the HORIZONS-AMI trial have been previously reported. In this final report, we aimed to assess 3-year outcomes...
- Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattleD Sa-Carvalho
Plum Island Animal Disease Center, Agricultural Research Service, North Atlantic Area, U S Department of Agriculture, Greenport, New York 11944, USA
J Virol 71:5115-23. 1997..viruses demonstrated that positively charged residues are required at both 2134 and 3056 for binding to heparin. Taken together, these results indicate that in vitro cultivation of FMDV type O selects viruses that bind to ..
- Sulfated K5 Escherichia coli polysaccharide derivatives as wide-range inhibitors of genital types of human papillomavirusDavid Lembo
Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano, Turin, Italy
Antimicrob Agents Chemother 52:1374-81. 2008..and biological assays to prepare a collection of sulfated K5 derivatives with a backbone structure resembling the heparin/heparan biosynthetic precursor and to test them for their anti-HPV activity...
- The heparin-binding domain confers diverse functions of VEGF-A in development and disease: a structure-function studyDominik Krilleke
Ocular Biology and Therapeutics, UCL Institute of Ophthalmology, 11 43 Bath Street, London EC1V 9EL, UK
Biochem Soc Trans 37:1201-6. 2009..isoforms of VEGF (vascular endothelial growth factor)-A, including VEGF(164(165)), contain a highly basic HBD (heparin-binding domain)...
- Antithrombin, heparin, and heparan sulfateSteven M Opal
Infectious Disease Division, Brown Medical School, Providence, RI 02860, USA
Crit Care Med 30:S325-31. 2002..The interaction between antithrombin and specific polysulfated, acidic oligosaccharide moieties found on heparin and related proteoglycan molecules within the circulation and on endothelial surfaces will also be examined.
- Heparin-bonded covered stents versus bare-metal stents for complex femoropopliteal artery lesions: the randomized VIASTAR trial (Viabahn endoprosthesis with PROPATEN bioactive surface [VIA] versus bare nitinol stent in the treatment of long lesions in supJohannes Lammer
Departments of Cardiovascular and Interventional Radiology, Medical University Vienna, Vienna, Austria Electronic address
J Am Coll Cardiol 62:1320-7. 2013..The hypothesis that endovascular treatment with covered stents has equal risks but higher efficacy than bare-metal stents (BMS) in long femoropopliteal artery disease was tested...
- Evaluation of the Becton-Dickinson rapid serum tube: does it provide a suitable alternative to lithium heparin plasma tubes?Goce Dimeski
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia
Clin Chem Lab Med 48:651-7. 2010..Although lithium heparin plasma has unique problems, it has become an alternative in hospital-based laboratories.
- Cell type-specific differences in glycosaminoglycans modulate the biological activity of a heparin-binding peptide (RKRLQVQLSIRT) from the G domain of the laminin alpha1 chainM P Hoffman
Craniofacial Developmental Biology and Regeneration Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892 4370, USA
J Biol Chem 276:22077-85. 2001..cell line (B16F10) both bind to the peptides, but cell attachment was inhibited by glycosaminoglycans, modified heparin, and sized heparin fragments in a cell type-specific manner...
- Heparin-coated superparamagnetic iron oxide for in vivo MR imaging of human MSCsJi Hye Lee
Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133 791, Republic of Korea
Biomaterials 33:4861-71. 2012..Here, we present superparamagnetic iron oxide (SPIO) nanoparticles coated with unfractionated heparin (UFH-SPIO) as a new negative contrast agent for in vivo MR imaging of hMSCs...
- His-384 allotypic variant of factor H associated with age-related macular degeneration has different heparin binding properties from the non-disease-associated formSimon J Clark
Medical Research Council MRC Immunochemistry Unit and Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, United Kingdom
J Biol Chem 281:24713-20. 2006..Here, using a recombinant construct, we show that amino acid 384 is adjacent to a heparin-binding site in CCP7 of factor H and demonstrate that the allotypic variants differentially recognize heparin...
- Effects of citrate acid concentrate (citrasate®) on heparin N requirements and hemodialysis adequacy: a multicenter, prospective noninferiority trialJeffrey J Sands
Fresenius Medical Care North America, Celebration, FL 34747, USA
Blood Purif 33:199-204. 2012..Previous studies suggest CD may improve dialysis adequacy and decrease heparin requirements, presumably due to nonsystemic anticoagulant effects in the dialyzer.
- Heparin-induced thrombocytopenia: in vitro studies on the interaction of dabigatran, rivaroxaban, and low-sulfated heparin, with platelet factor 4 and anti-PF4/heparin antibodiesKrystin Krauel
Institut für Immunologie und Transfusionsmedizin, Universitä Greifswald, Sauerbruchstrasse, Greifswald, Germany
Blood 119:1248-55. 2012b>Heparin is a widely used anticoagulant. Because of its negative charge, it forms complexes with positively charged platelet factor 4 (PF4). This can induce anti-PF4/heparin IgG Abs...
- Synthesis of heparin oligosaccharides and their interaction with eosinophil-derived neurotoxinShang Cheng Hung
Genomics Research Center, Academia Sinica, Taipei, Taiwan
Org Biomol Chem 10:760-72. 2012A convenient route for the synthesis of heparin oligosaccharides involving regioselective protection of D-glucosamine and a concise preparation of rare L-ido sugars from diacetone α-D-glucose is described...
- Effect of heparin on neuroprotection against spinal cord ischemia and reperfusion in ratsK Korkmaz
Department of Cardiovascular Surgery, Ankara Numune Educational and Research Hospital, Turkey
Eur Rev Med Pharmacol Sci 17:522-30. 2013..We investigated the effect of the degree of anticoagulation on spinal cord I/R injury and whether heparin is protective against I/R injury beside its anticoagulant properties.
- Induction of heparin-binding EGF-like growth factor expression during myogenesis. Activation of the gene by MyoD and localization of the transmembrane form of the protein on the myotube surfaceX Chen
Department of Surgery, Children s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 270:18285-94. 1995b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) gene expression and protein localization were analyzed during the process of myogenic differentiation. The mouse HB-EGF gene was isolated, and a 1...
- Early intravenous unfractionated heparin and mortality in septic shockRyan Zarychanski
Section of Critical Care Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
Crit Care Med 36:2973-9. 2008..Unfractionated heparin is a known anticoagulant, which also possesses anti-inflammatory properties...
- Comparison of efficacy of silicone gel, silicone gel sheeting, and topical onion extract including heparin and allantoin for the treatment of postburn hypertrophic scarsHuseyin Karagoz
Maresal Cakmak Military Hospital, Department of Plastic and Reconstructive Surgery, Erzurum, Turkey
Burns 35:1097-103. 2009..the efficacy of silicone gel (Scarfade), silicone gel sheet (Epi-Derm), and topical onion extract including heparin and allantoin (Contractubex) for the treatment of hypertrophic scars...
- Hyaluronic acid-based hydrogels functionalized with heparin that support controlled release of bioactive BMP-2Gajadhar Bhakta
Institute of Medical Biology, A STAR, 8A Biomedical Grove, 06 06 Immunos, Singapore 138648, Singapore
Biomaterials 33:6113-22. 2012..b>Heparin (HP), a highly sulfated glycosaminoglycan (GAG) that avidly binds BMP-2, has inherent biological properties that ..
- Rapid induction of heparin-binding epidermal growth factor/diphtheria toxin receptor expression by Raf and Ras oncogenesS A McCarthy
DNAX Research Institute, Palo Alto, California 94304, USA
Genes Dev 9:1953-64. 1995..Through this approach the gene encoding heparin-binding epidermal growth factor (HB-EGF) was identified as an immediate-early transcriptional target of oncogenic ..
- Impact of intravenous heparin on quantification of circulating microRNAs in patients with coronary artery diseaseD Kaudewitz
Prof Manuel Mayr, King s British Heart Foundation Centre, King s College London, London, SE5 9NU, UK, Tel 44 02078 485132, Fax 44 2078485296, E mail
Thromb Haemost 110:609-15. 2013..Circulating levels of microRNAs have been measured in various studies related to cardiovascular disease. Heparin is a potential confounder of microRNA measurements due to its known interference with polymerase chain reactions...
- Regional citrate versus systemic heparin for anticoagulation in critically ill patients on continuous venovenous haemofiltration: a prospective randomized multicentre trialGerd R Hetzel
Department of Nephrology, Medical Faculty, Heinrich Heine University Dusseldorf, Germany
Nephrol Dial Transplant 26:232-9. 2011..Several protocols avoiding heparin and using regional citrate anticoagulation have been developed to diminish bleeding risks...
- Therapeutic use of heparin beyond anticoagulationRalf J Ludwig
Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23562 Lübeck, Germany
Curr Drug Discov Technol 6:281-9. 2009b>Heparin has been used as an anticoagulant for decades. Recently, attention has been drawn to the non-anticoagulant activities of heparin...
- Effects of heparin and related molecules upon neutrophil aggregation and elastase release in vitroRachel A Brown
Sackler Institute of Pulmonary Pharmacology, Guy s, King s and St Thomas School of Biomedical Sciences, King s College London, London
Br J Pharmacol 139:845-53. 2003..b>Heparin inhibits the enzymatic activity of elastase and here we provide evidence for the first time that heparin can ..
- Identification of fibroblast growth factor 9 (FGF9) as a high affinity, heparin dependent ligand for FGF receptors 3 and 2 but not for FGF receptors 1 and 4D Hecht
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel
Growth Factors 12:223-33. 1995Fibroblast growth factors (FGF) are multifunctional, heparin binding polypeptides that share structural similarity, but differ in their target cell specificity and expression pattern...
- Up-regulation of EGF receptor and its ligands, AREG, EREG, and HB-EGF in oral lichen planusKenichi Kumagai
Department of Oral and Maxillofacial Surgery, Tsurumi University, Tsurumi ku, Yokohama, Japan
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110:748-54. 2010..This study aimed to investigate the roles of the epidermal growth factor receptor (EGFR) family members and their ligands in oral lichen planus (OLP)...
- Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironmentsDavid J Mahoney
Medical Research Council Immunochemistry Unit, University of Oxford, United Kingdom
J Biol Chem 280:27044-55. 2005..Here we show that this domain can also associate with the glycosaminoglycan heparin/heparan sulfate...
- Clinical review: anticoagulation for continuous renal replacement therapy--heparin or citrate?Heleen M Oudemans-van Straaten
Department of Intensive Care Medicine, Onze Lieve Vrouwe Gasthuis, Oosterpark 9, 1091 AC Amsterdam, The Netherlands
Crit Care 15:202. 2011b>Heparin is the most commonly prescribed anticoagulant for continuous renal replacement therapy. There is, however, increasing evidence questioning its safety, particularly in the critically ill...
- Mycoplasma hyopneumoniae Surface proteins Mhp385 and Mhp384 bind host cilia and glycosaminoglycans and are endoproteolytically processed by proteases that recognize different cleavage motifsAnia T Deutscher
NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden NSW 2567, Australia
J Proteome Res 11:1924-36. 2012..b>Heparin- and cilium-binding sites were identified within P60(384), P50(384), and P88(385)...
- Contaminated heparin associated with adverse clinical events and activation of the contact systemTakashi Kei Kishimoto
Momenta Pharmaceuticals, the Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Koch Institute for Integrative Cancer Research, Cambridge, MA 02139, USA
N Engl J Med 358:2457-67. 2008There is an urgent need to determine whether oversulfated chondroitin sulfate (OSCS), a compound contaminating heparin supplies worldwide, is the cause of the severe anaphylactoid reactions that have occurred after intravenous heparin ..
- In vivo distribution and antitumor activity of heparin-stabilized doxorubicin-loaded liposomesHee Dong Han
Medicinal Science Division, Korea Research Institute of Chemical Technology, P O Box 107, Yuseong, Daejeon 305 600, South Korea
Int J Pharm 313:181-8. 2006The purpose of this study was to investigate the effect of heparin conjugation to the surface of doxorubicin (DOX)-loaded liposomes on the circulation time, biodistribution and antitumor activity after intravenous injection in murine ..
- Role of cellular glycosaminoglycans and charged regions of viral G protein in human metapneumovirus infectionSutthiwan Thammawat
Department of Microbiology and Infectious Diseases, Flinders Medical Centre, Bedford Park, SA 5042, Australia
J Virol 82:11767-74. 2008..The pretreatment of hMPV with soluble heparin markedly inhibited the infection of HEp-2 cells...
- Cell transplantation of adipose tissue-derived stem cells in combination with heparin attenuated acute liver failure in miceHiroshi Yukawa
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461 0047, Japan
Cell Transplant 18:611-8. 2009The effect of adipose tissue-derived stem cells (ASCs) in combination with heparin transplantation on acute liver failure mice with carbon tetrachloride (CCl(4)) injection was investigated...
- Characterization of the Mycobacterium avium subsp. paratuberculosis laminin-binding/histone-like protein (Lbp/Hlp) which reacts with sera from patients with Crohn's diseaseLouise H Lefrançois
INRA, UR1282, Infectiologie Animale, Santé Publique IASP 311, F 37380 Nouzilly, France
Microbes Infect 13:585-94. 2011..A Map protein was isolated by heparin-Sepharose chromatography and identified as a laminin-binding protein like...
- Structural basis for selective vascular endothelial growth factor-A (VEGF-A) binding to neuropilin-1Matthew W Parker
Department of Molecular and Cellular Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536, USA
J Biol Chem 287:11082-9. 2012..We have determined the crystal structure of the exon 7/8-encoded VEGF-A heparin binding domain in complex with the Nrp1-b1 domain...
- Antimetastatic activities of heparins and modified heparins. Experimental evidenceLubor Borsig
Institute of Physiology, University of Zürich and Zürich Center for Integrative Human Physiology, Zurich, Switzerland
Thromb Res 125:S66-71. 2010b>Heparin is commonly used for prevention or treatment of cancer-associated thromboembolism. Recent clinical evidence indicates that heparin, and low-molecular weight heparin improves survival of cancer patients...
- Heparin inhibits ligand binding to the leukocyte integrin Mac-1 (CD11b/CD18)K Peter
Department of Internal Medicine III, University of Heidelberg, Germany
Circulation 100:1533-9. 1999The clinical benefits of heparin reach beyond its anticoagulative properties. Recently, it has been described that leukocytes adhere on immobilized heparin mediated by the integrin Mac-1 (CD11b/CD18, alphaMbeta2, or CR3)...
- Identification of a linear heparin binding domain for human respiratory syncytial virus attachment glycoprotein GS A Feldman
Laboratory of Pediatric and Respiratory Virus Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
J Virol 73:6610-7. 1999..Infection is mediated, in part, by an initial interaction between attachment protein (G) and a highly sulfated heparin-like glycosaminoglycan (Gag) located on the cell surface...
- Semi-rigid solution structures of heparin by constrained X-ray scattering modelling: new insight into heparin-protein complexesSanaullah Khan
Department of Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK
J Mol Biol 395:504-21. 2010The anionic polysaccharides heparin and heparan sulphate play essential roles in the regulation of many physiological processes. Heparin is often used as an analogue for heparan sulphate...
- Recent progress and applications in glycosaminoglycan and heparin researchTatiana N Laremore
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Curr Opin Chem Biol 13:633-40. 2009b>Heparin, the focus of this review, is a crucially important anticoagulant drug produced from animal sources, which was contaminated last year leading to a number of adverse side effects, some resulting in death...
- The structure of the glial cell line-derived neurotrophic factor-coreceptor complex: insights into RET signaling and heparin bindingVimal Parkash
Institute of Biotechnology, University of Helsinki, Helsinki, Finland
J Biol Chem 283:35164-72. 2008..We have solved the GDNF(2).GFR alpha 1(2) complex structure at 2.35 A resolution in the presence of a heparin mimic, sucrose octasulfate. The structure of our GDNF(2)...
- The characteristics, biodistribution and bioavailability of a chitosan-based nanoparticulate system for the oral delivery of heparinMei Chin Chen
Department of Chemical Engineering, National Cheng Kung University, Tainan City, Taiwan, ROC
Biomaterials 30:6629-37. 2009b>Heparin is a potent anticoagulant; however, it is poorly absorbed in the gastrointestinal tract...
- Heparin and heparan sulfate: structure and functionDallas L Rabenstein
Department of Chemistry, University of California, Riverside 92521, USA
Nat Prod Rep 19:312-31. 2002This review covers the structure and function of heparin and heparan sulfate glycosaminoglycans...
- Heparin-degrading sulfatases in hepatocellular carcinoma: roles in pathogenesis and therapy targetsJin Ping Lai
Center for Basic Research in Digestive Diseases, Miles and Shirley Fiterman Center for Digestive Diseases, Mayo Clinic College of Medicine and Mayo Clinic Cancer Center, 200 First Street, SW, Rochester, MN 55905, USA
Future Oncol 4:803-14. 2008..Two recently identified human heparin-degrading endosulfatases, named sulfatase 1 (SULF1) and sulfatase 2 (SULF2), have been found to be involved in ..
- Quantitative characterization of heparin binding to Tau protein: implication for inducer-mediated Tau filament formationHai Li Zhu
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
J Biol Chem 285:3592-9. 2010..Polyanionic cofactors such as heparin can induce Tau filament formation in vitro...
- Roles of heparan-sulphate glycosaminoglycans in cancerRam Sasisekharan
Biological Engineering Division and Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nat Rev Cancer 2:521-8. 2002
- Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell linesYuri Terao-Muto
Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Sirokanedai, Minato ku, Tokyo 108 8639, Japan
Antiviral Res 80:370-6. 2008..Soluble heparin (HP) inhibited the rMV-EGFP infectivity in SLAM-negative cell lines in a dose-dependent manner...
- Antithrombin-S195A factor Xa-heparin structure reveals the allosteric mechanism of antithrombin activationDaniel J D Johnson
Department of Haematology, Division of Stuctural Medicine, Thrombosis Research Unit, Cambridge Institute for Medical Research, University of Cambridge, UK
EMBO J 25:2029-37. 2006..One of the principal regulatory mechanisms involves heparin activation of the serpin antithrombin (AT)...
- Heparin, heparan sulfate and heparanase in inflammatory reactionsJin ping Li
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
Thromb Haemost 102:823-8. 2009..b>Heparin, a classical anticoagulant, exhibits anti-inflammatory effects in animal models and in the clinic, presumably ..
- Structure of heparin-binding EGF-like growth factor. Multiple forms, primary structure, and glycosylation of the mature proteinS Higashiyama
Department of Surgical Research, Children s Hospital, Boston, Massachusetts
J Biol Chem 267:6205-12. 1992b>Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a newly described member of the epidermal growth factor (EGF) family that is mitogenic for BALB/c 3T3 cells, inhibits the binding of 125I-EGF to its receptor, and ..
- Comparison of effects on markers of blood cell activation of enoxaparin, dalteparin, and unfractionated heparin in patients with unstable angina pectoris or non-ST-segment elevation acute myocardial infarction (the ARMADA study)Gilles Montalescot
Institut de Cardiologie, Bureau 2 236, Groupe Hospitalier Pitié Salpêtrière Hospital, AP HP, 47 Boulevard de l Hopital, 75013 Paris, France
Am J Cardiol 91:925-30. 2003..LMWHs) enoxaparin and dalteparin have shown superior and equivalent efficacy, respectively, over unfractionated heparin (UFH) in patients with unstable angina pectoris (UAP) or non-ST-segment elevation myocardial infarction (NSTEMI)...
- Assessment of the heparin-binding AN69 ST hemodialysis membrane: II. Clinical studies without heparin administrationSylvie Lavaud
Service de Nephrologie, Centre Hospitalier et Universitaire and CNRS FRE 2534, Reims, France
ASAIO J 51:348-51. 2005Binding polyanionic unfractionated heparin over the modified AN69 polyacrylonitrile membrane, the surface electronegativity of which has been neutralized by polyethyleneimine (AN69-ST), renders the membrane more hemocompatible...
- Heparin-binding EGF-like growth factor: characterization of rat and mouse cDNA clones, protein domain conservation across species, and transcript expression in tissuesJ A Abraham
Scios Nova Inc, Mountain View, CA 94043
Biochem Biophys Res Commun 190:125-33. 1993Clones were obtained that encode the rat and mouse forms of heparin-binding EGF-like growth factor (HB-EGF), a potent mitogen for smooth muscle cells, fibroblasts and keratinocytes that is proposed to be derived from a transmembrane ..
- Heparin attenuates cytotoxic and inflammatory activity of Alzheimer amyloid-beta in vitroLuigi Bergamaschini
Department of Internal Medicine, Ospedale Maggiore IRCCS, University of Milan, Italy
Neurobiol Aging 23:531-6. 2002..We evaluated the effect of heparin on Abeta (1-42) neurotoxicity and on its ability to activate complement and contact system...
- Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligandsNarayanam V Rao
Department of Internal Medicine, University of Utah Medical Center, Salt Lake City, UT 84132, USA
Am J Physiol Cell Physiol 299:C97-110. 2010While heparin has been used almost exclusively as a blood anticoagulant, important literature demonstrates that it also has broad anti-inflammatory activity...
- Delineating mechanisms of dissociation for isomeric heparin disaccharides using isotope labeling and ion trap tandem mass spectrometryOla M Saad
Department of Chemistry, University of California at Berkeley, Berkeley, California 94720 1460, USA
J Am Soc Mass Spectrom 15:1274-86. 2004b>Heparin and heparan sulfate (HS) glycosaminoglycans have been identified as important players in many physiological as well as pathophysiological settings...
- A novel anti-angiogenic form of antithrombin with retained proteinase binding ability and heparin affinityH Larsson
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden
J Biol Chem 276:11996-2002. 2001Latent antithrombin, an inactive antithrombin form with low heparin affinity, has previously been shown to efficiently inhibit angiogenesis and tumor growth...
- Anti-Inflammatory Glycosaminoglycan Ethers for Treatment of PeriodontitisJUSTIN RODNEY SAVAGE; Fiscal Year: 2011..Sulfated glycosaminoglycans (GAGs, e.g., heparin) block ligation of RAGE by AGE and its other ligands...
- Cyr61/CCN1-Induced Angiogenesis and Vasculogenesis in the RetinaBrahim Chaqour; Fiscal Year: 2010..work in the PI's laboratory have revealed that the cysteine-rich protein 61 (Cyr61), a novel ECM-associated, heparin- and integrin-binding protein, stimulates the angiogenic phenotype of the BM-derived hematopoeitic progenitors, ..
- Hepatic Lipase and HCV InfectionTianyi Wang; Fiscal Year: 2010..enzymes in maintaining circulating lipoprotein levels and can facilitate lipid uptake via their associations with heparin sulphate proteoglycans (HSPG) on hepatocyte surface...
- STRUCTURE AND FUNCTION OF HEPARIN COFACTOR IIDouglas M Tollefsen; Fiscal Year: 2010b>Heparin cofactor II (HCII) is an inhibitor of thrombin in plasma that can be activated by dermatan sulfate (DS) proteoglycans on vascular smooth muscle cells and fibroblasts...
- Adjunctive Use of Apyrase to Fibrinolytic TherapyRidong Chen; Fiscal Year: 2013..The current regimen, including tissue plasminogen activator, aspirin, clopidogrel and heparin, still induces inadequate coronary reperfusion in 30-40% of patients and early thrombotic reocclusion in 5-10% ..
- Novel Glycosaminoglycan Ethers for Prevention of MetastasisAlana L Welm; Fiscal Year: 2011DESCRIPTION (provided by applicant): Heparin and its derivatives block P- and L-selectin mediated metastatic spread of cancer in animal models, and they potently inhibit the matrix degrading enzyme heparanase...
- GPCR Signaling &Vascular Wall RemodelingGadiparthi N Rao; Fiscal Year: 2013..While protease-dependent shedding of heparin-binding growth factors, in this case, heparin-binding epidermal growth factor (HB-EGF), appears to be accountable ..
- Extracellular Matrix Remodeling During AgingJANE M SOTTILE; Fiscal Year: 2010..the deposition and turnover of other ECM molecules including collagen I, collagen III, thrombospondin, and heparin sulfate proteoglycans...
- FEASIBILITY OF REPAIRING GBM DEFECTS IN VIVOJeffrey H Miner; Fiscal Year: 2012..are laminin-521 (alpha5beta2gamma1), type IV collagen (the alpha3/4/5 network), nidogens-1 and -2, and the heparin sulfate proteoglycan agrin...
- Molecular mechanism of antiangiogenic properties of gold nanoparticlePriyabrata Mukherjee; Fiscal Year: 2013..We have recently demonstrated that "bare" AuNPs bind to heparin binding growth factors such as VPF/VEGF165, bFGF and PlGF through their heparin-binding domain and inhibit their ..
- Heparan Sulfate Proteoglycans in Human HematopoiesisPankaj Gupta; Fiscal Year: 2013..Gremlin1 is an extracellular heparin-binding BMP inhibitor that plays a critical role in growth, differentiation and embryonic development and in ..
- Novel Glycosaminoglycan Derivatives for Treatment of Bladder InflammationJUSTIN RODNEY SAVAGE; Fiscal Year: 2011..First, heparin is administered intravesically, but the anti-coagulant properties and expense limit its regular usage...
- Dysregulation of the Tumor Microenvironment in Hepatocellular CarcinomaLewis R Roberts; Fiscal Year: 2010..Stellate cell activation is dependent on signaling by platelet-derived growth factor (PDGF), a heparin-binding growth factor, and transforming growth factor-[unreadable] (TGF-[unreadable]) which is stored in the ECM ..
- New Pathways in Thrombosis and Inflammation Mediated by Semaphorin-Plexin SignaliGuy A Zimmerman; Fiscal Year: 2010..therapies including cyclooxygenase inhibitors (aspirin), thienopyridines (clopidogrel), and anticoagulants (heparin, warfarin)...
- Novel Biomarkers Predictive of Heparin-Induced ThrombocytopeniaBarbara A Konkle; Fiscal Year: 2010..Thrombosis is the major cause of death in the United States and heparin, in unfractionated (UFH) or low molecular weight form, is uniformly used for anticoagulation in the hospital ..
- Insulin Resistance in Chronic Heart Failure: pathophysiology and potential for reNir Uriel; Fiscal Year: 2013..We will also measure post heparin plasma lipoprotein lipase (LPL) activity to assess the availability of circulating lipoprotein-derived FFA in ..
- The role of Histidine rich protein II in cerebral malariaPriya Pal; Fiscal Year: 2013..Previous studies from our laboratory show that HRPII binds to soluble glycosaminoglycans (GAGs) such as heparin, heparan sulfate, and dermatan sulfate with high affinity...
- Role of Maternal Platelets in the Placenta and Pregnancy ComplicationsRashmi Sood; Fiscal Year: 2013..find that this model is driven by thrombin-mediated platelet activation and that pregnancy outcome is improved by heparin treatment, but not by treatment with hirudin, fondaparinux or a direct Xa inhibitor...
- Metabolic Engineering of Chinese Hamster Ovary Cells for Production of HeparinSusan T Sharfstein; Fiscal Year: 2011b>Heparin is the most widely used anticoagulant drug in modern medicine...
- The central role of c-Met in H. pylori pathogenesis.Sarah E Keates; Fiscal Year: 2010..Disintegrin And Metallopeptidase domain-17 (ADAM-17);and that iii) activation of ADAM-17 can mediate shedding of heparin-binding epidermal growth factor (HB-EGF) which in turn culminates in EGFR transactivation...
- Cell-synthesized Thread-based Tissue EngineeringNICOLAS L'HEUREUX; Fiscal Year: 2012..As a cost-effective alternative, we will explore the possibility of replacing it by a new type of heparin coating...
- Physiology and Molecular Biology of Factor XIDavid Gailani; Fiscal Year: 2013..In Aim 3, we will determine the importance of the fXI anion binding sites to heparin-dependent regulation of fXIa by plasma serpins, and evaluate the capacity of poly-P to down-regulate serpin-..
- Apolipoprotein A-V: A Functional Proteomics StudyROBERT O apos MARA RYAN; Fiscal Year: 2010..553G>T SNP will be characterized for intra- molecular disulfide bond formation, heparin / receptor binding interactions and in vivo TG modulation capability...
- GE Healthcare Fast Protein Liquid Chromatography FPLC product number 13470501.Jasti S Rao; Fiscal Year: 2010..b>heparin for AAV2 and AAV6). The packing material used, called the affinity matrix, must be inert and easily modified...
- Designing a Microenvironment Niche for Liver-Specific Differentiation of hESCsAlexander Revzin; Fiscal Year: 2013..Stem cell-carrying heparin hydrogel microstructures will be implanted in immunodeficient mice and survival/function of cells within these ..
- FIBRONECTIN REGULATION OF CARCINOMA APOPTOSISYvonne Kapila; Fiscal Year: 2009..We have determined that the carboxyl-terminal heparin-binding domain and alternatively spliced V region of FN are important to this process, since a FN miniprotein (V+..
- Extracellular Sulfatases As Novel Biomarkers For Early Detection of CancerJoanna Phillips; Fiscal Year: 2013..Aim 4: Characterize the plasma levels of heparin-binding factors (growth factors, morphogens, cytokines, chemokines) that are potentially mobilized by SULF action ..
- Extracellular Matrix Components, Oxidants and Antioxidants in Pulmonary FibrosisCORRINE R KLIMENT; Fiscal Year: 2010..the lung from oxidant-induced damage, inflammation, and fibrosis is by preventing oxidative fragmentation of Heparin/Heparan Sulfate (HS) in the ECM...
- SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANSRobert J Linhardt; Fiscal Year: 2012..e., chondroitin, dermatan, heparin, etc.), molecular weight, charge density and disaccharide analysis...
- MOLECULAR BASIS OF BLOOD COAGULATION REGULATIONSteven T Olson; Fiscal Year: 2013..recent studies have suggested an important revision of the allosteric mechanism of activation of this serpin by heparin in showing that activation is mediated principally by the relief of repulsive interactions with factor Xa and ..
- MOLECULAR &CELLULAR MECHANISMS IN TRANSFUSION MEDICINEPeter J Newman; Fiscal Year: 2013..Wang, seeks to define the role of specific B lymphocyte populations in the cellular immune response underlying heparin-induced thrombocytopenia - studies that may transform our ability to diagnose and manage this complex bleeding ..
- Role of heparin binding growth factors in vascular leakage and fatal bleedingPatricio E Ray; Fiscal Year: 2012Very little is known about the role that circulating heparin binding angiogenic growth factors play in the pathogenesis of bleeding disorders induced by heparin-like drugs or heparinoids in critically ill children...
- Reduction of Tunneled Dialysis Catheter Dysfunction Via Long Term Nitric Oxide RePrabir Roy-Chaudhury; Fiscal Year: 2013..47,000 to the cost of care and a 14.6 d increase in length of stay. Current heparin, silver impregnated, or antibiotic coated TDCs are either expensive, non-effective, or suffer from mechanical ..
- Serpin Regulation of Coagulation ProteasesAlireza R Rezaie; Fiscal Year: 2013..AT is a relatively weak inhibitor of coagulation proteases unless it binds to the heparin-like glycosaminoglycans lining the microvasculature or therapeutically administered for prophylaxis and treatment ..
- Factor XI inhibitor for thrombosisERIK IAN TUCKER; Fiscal Year: 2013..significant antithrombotic effects that are comparable to a high interventional dose of the low-molecular-weight heparin enoxaparin (Lovenox(R), 1mg/kg, iv), and 3) Verifying that the antithrombotic dose of AXIMAB 1A6 produces no ..
- Structure-Function of AAV - a Viral Gene Therapy VectorMichael S Chapman; Fiscal Year: 2013..to characterize the diversity of HS interactions that has evolved in AAV, correlating structure with measured heparin affinity, cell attachment and cell entry, measured by in vitro transgene expression...
- Regulation and Function of Immunoglobulin D in Mucosal Immune DefenseAndrea Cerutti; Fiscal Year: 2013..by activating local and systemic innate immune cells such as basophils and mast cells through interaction with heparin, eosinophil cationic protein and inflammasomes. Three aims are proposed...
- Heparan sulfate proteoglycans in aging and developmentGREGORY COLE; Fiscal Year: 2009..These studies will focus on analyzing the role agrin plays in modulating the function of heparin-binding signaling molecules such as the fibroblast growth factors (FGFs)...
- 2012 New Insights into CoagulationEleanor Pollak; Fiscal Year: 2012..Other topics that we plan to hear discussed include the older well-established coagulation agents: heparin and low-molecular weight heparin, warfarin, and intravenous direct thrombin inhibitors...
- Therapeutic factor XI blockade for sepsisERIK IAN contact TUCKER; Fiscal Year: 2010..by antibody inhibition of FXI is antithrombotic for more than a week, and hemostatically safer than heparin or aspirin...
- A murine model for human factor H R1210C mutation-related diseasesWenchao Song; Fiscal Year: 2013..The R1210C variant of human fH showed reduced binding to C3b/C3d, heparin and endothelial cells, yet R1210 is not conserved across species (D1210 in mouse, G1210 in rat, P1210 in cows and ..
- HEPylated G-CSF: Drug with Safer, Enhanced Delivery for Neutropenia TreatmentWei Jing; Fiscal Year: 2011..Heparosan, a new type of therapeutic modifying agent, is a natural "self" polysaccharide related to heparin. Caisson's technology also allows for synthesis of sugar polymers with a very narrow size distribution and ..
- Pregnancy specific glycoprotein 1 activates transforming growth factor betaGabriela S Dveksler; Fiscal Year: 2013..Recently, we described that besides its immune regulatory function, PSG1 is pro-angiogenic. PSG1 binds to the heparin and chondroitin sulfate in syndecans and this interaction results in the formation of capillary-like structures ..
- Microthrombus Formation Triggers Lung Injury in SepsisBRUCE ALLAN HARMS; Fiscal Year: 2012..of thrombus formation prevents neutrophil sequestration, and prevents lung injury: low molecular weight heparin, recombinant hirudin, and recombinant activated protein C have anti-thrombotic properties that prevent ..
- Genetic influences in experimental pancreatitisBishr Omary; Fiscal Year: 2013..to experimental pancreatitis (Aim 3);and (iii) Testing the effect of targeting the coagulation pathway, via heparin, as a therapeutic modality in experimental pancreatitis (Aim 4)...
- Pathogenesis of HIV-HUS in childrenPatricio E Ray; Fiscal Year: 2012..In aim 1 we will define how FGF-2, BP-1, HIV-Tat , VEGF-A, and heparin, alone or in combination, modulate the permeability of cultured human glomerular endothelial cells and determine ..
- Improving the diagnosis of heparin-induced thrombocytopeniaAdam Cuker; Fiscal Year: 2013DESCRIPTION (provided by applicant): Heparin-induced thrombocytopenia (HIT) is a potentially fatal prothrombotic complication of heparin therapy...
- LPD Nanoparticles in Anti-Cancer TherapyLeaf Huang; Fiscal Year: 2012..In aim 3, we will employ another nanoparticle formulation, i.e., LPH, which contains heparin sulfate to deliver doxorubicin (dox), a potent anti-cancer drug, either by itself or together with EGFR siRNA...
- Mechanisms of normal and abnormal platelet homeostasisJoel S Bennett; Fiscal Year: 2010..Project 1, entitled "Understanding the mechanistic basis of heparin-induced thrombocytopenia (HIT)", tests the hypothesis that variations in platelet factor 4 (PF4) secretion are ..
- CHEMISTRY AND BIOLOGY OF HEPARAN SULFATEUMESH RAMANLAL DESAI; Fiscal Year: 2013..with proteins using computational, biochemical and biophysical technologies;designing specific GAG activators of heparin cofactor II;and developing selected GAGs as clinically useful anticoagulants;Project III (PL: K...
- The Role of the EGF Receptor in Acute and Chronic Renal InjuryRaymond Harris; Fiscal Year: 2013..the current funding period have indicated that the membrane-associated form of EGFR ligands, and specifically heparin-binding EGF-like growth factor (HB-EGF), subserves different functions than the soluble mature form of the ..
- Biomimetic Engineering of Vascular ProsthesesRoger E Marchant; Fiscal Year: 2012..for 1v23 (compared to 1IIb23), REDV peptide with specificity for 1421, CRRETAWAC peptide specific for 1521, and heparin binding peptides that demonstrate specificity for syndecans...
- Saturated Fat and Protein Effects on Atherogenic DyslipidemiaRonald M Krauss; Fiscal Year: 2013..for possible metabolic determinants of dietary effects on apoB-containing lipoprotein subclasses, including post-heparin plasma hepatic lipase activity, which is critical for production of smaller LDL, and LDL receptor activity as ..
- Potential of a dominant-negative FGF mutant as a therapeutic in cancerYoshikazu Takada; Fiscal Year: 2012..an FGF1 mutant (Arg-50 to Glu, R50E) that is defective in integrin binding but still binds to FGF-receptor or heparin. R50E was defective in inducing FGF signaling while it binds to heparin or FGFR, suggesting that integrin binding ..
- GPx-3 and Peroxide Flux in the Endothelial CellJoseph Loscalzo; Fiscal Year: 2013..with complex and unique translational requirements;demonstration of the cofactor activation of GPx-3 catalysis by heparin via a unique polyanionic glycosaminoglycan binding site in the solvent-exposed carboxyterminal tail;the ..
- Identification of novel genes regulating metalloproteinase activityAndreas Herrlich; Fiscal Year: 2009..In the kidney, the EGF ligand HB (heparin-binding)-EGF is involved in tubular repair after injury...
- Hypertensive Mechanisms in PreeclampsiaERIC MATTHEW GEORGE; Fiscal Year: 2013..One potential mechanism is suggested through the presence of a heparin binding site on the sFlt-1 protein and recent evidence from the literature that the switch between local ..
- Development of B-Lock an antibiofilm catheter lock productKrzysztof Appelt; Fiscal Year: 2010..Conduct a randomized, double-blind clinical trial to evaluate B-LockTM solution compared with heparin lock solution in the preservation of in-dwelling catheter sterility and potency in patients receiving ..
- Regulation of heparanase in cancer progressionIsrael Vlodavsky; Fiscal Year: 2013..e., chemically modified non-anticoagulant species of heparin, siRNA), antibodies directed against specific domains of the heparanase protein, assay systems (i.e...
- "New Needle for Two-Needle Hemodialysis"ALFREDO R ZARATE; Fiscal Year: 2011..HD prescription during both HD including length of dialysis, type of dialyzer non-reused, size of needles (15g), heparin, dialysate composition, and blood and dialysate flow rate...
- Structural and Biophysical Characterization of Hedgehog SignalingDaniel J Leahy; Fiscal Year: 2013..Hh:Cdo, and Hh:Boc complexes, and demonstrated that these interactions depend on additional co-factors including heparin and Ca2+. We have also recently expressed and purified small amounts (~40 ug) of Patched...
- Structural and functional characterization of the receptor protein tyrosine phospHeidi Erlandsen; Fiscal Year: 2010..has previously been described mainly in neuronal cells, and was the first described receptor for the heparin-binding molecule pleiotrophin (PTN), a small chemokine of 15- 17 kDa, that was also independently identified as ..
- Long Circulating Low Molecular Weight Heparins PulmonaryFakhrul Ahsan; Fiscal Year: 2006..Drugs that have traditionally been used for the short and long term treatment of VTE include unfractionated heparin and warfarin...
- SYNTHESIS AND TESTING OF A NEW CLASS OF HEPARINOIDSJoseph Toce; Fiscal Year: 1991..Abstract): This is a phase I application in which the goal is to develop a therapeutic agent which would replace heparin. Although heparin is the best drug for treatment of thromboembolic disease, it is unstable if administered orally ..
- Structural Mechanisms for the Inhibition of ThrombosisJAMES HUNTINGTON; Fiscal Year: 2009..inhibition of upstream coagulation factor Xa;2) direct inhibition of thrombin by serpins antithrombin (AT) and heparin cofactor II (HCII);and, 3) attenuation of thrombin's procoagulant activity through cofactor binding and ..
- Mechanisms of Hemostatic Protease Inhibition by SerpinsIngrid M Verhamme; Fiscal Year: 2010The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
- HEPARIN INDUCED THROMBOCYTOPENIA/THROMBOSISGIAN VISENTIN; Fiscal Year: 2000b>Heparin-induced thrombocytopenia/thrombosis (HITP) is an important cause of morbidity and mortality in patients receiving heparin therapy...
- Etiologic and therapeutic studies of heparin-induced thrombocytopenia (HIT)Mortimer Poncz; Fiscal Year: 2011b>Heparin-induced thrombocytopenia (HIT) is an iatrogenic cause of thrombocytopenia and thrombosis due to antibodies to complexes between Platelet Factor 4 (PF4) and heparin...
- Antithrombotic Activity of Ascidian GlycosaminoglycansDOUGLAS TOLLEFSEN; Fiscal Year: 2004DESCRIPTION (provided by applicant) Heparin from porcine or bovine tissues is used to treat diseases such as venous thrombosis, pulmonary embolism, and coronary artery thrombosis...
- MOLECULAR MECHANISM OF THROMBIN INHIBITIONScott Cooper; Fiscal Year: 2003..Previously we made the observation that, unlike AT, PCI is a potent inhibitor of thrombin bound to TM. The heparin binding domains of PCI and AT also differ; in AT the D-helix is a major part of the heparin-binding domain, while ..
- Biomedical Polymers With Heparin-Binding End GroupsRobert Ward; Fiscal Year: 2004Endpoint immobilization of heparin on the surface of polymeric biomaterials is a well-established method for improving thrombo-resistance...
- Development of biomimetic oligomers as anti-coagulant antagonistsRichard Scott; Fiscal Year: 2007unreadable] DESCRIPTION (provided by applicant): Unfractionated heparin (UFH) and the low molecular weight heparins (LMWHs) are widely used anti-coagulants employed in a number of clinical and surgical applications...
- NMR STRUCTURES OF PLATELET FACTOR 4 & B-THROMBOGLOBULINKevin Mayo; Fiscal Year: 1992Platelet factor (PF4) and B-thromboglobulin-related proteins (BTG) are low- molecular weight heparin binding proteins generally considered to be platelet specific and are involved in blood clotting, wound healing, tissue repair and cell ..