scorpion venoms

Summary

Summary: Venoms from animals of the order Scorpionida of the class Arachnida. They contain neuro- and hemotoxins, enzymes, and various other factors that may release acetylcholine and catecholamines from nerve endings. Of the several protein toxins that have been characterized, most are immunogenic.

Top Publications

  1. pmc SdPI, the first functionally characterized Kunitz-type trypsin inhibitor from scorpion venom
    Ruiming Zhao
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People s Republic of China
    PLoS ONE 6:e27548. 2011
  2. pmc Hg1, novel peptide inhibitor specific for Kv1.3 channels from first scorpion Kunitz-type potassium channel toxin family
    Zong Yun Chen
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    J Biol Chem 287:13813-21. 2012
  3. ncbi Structural basis of a potent peptide inhibitor designed for Kv1.3 channel, a therapeutic target of autoimmune disease
    Song Han
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    J Biol Chem 283:19058-65. 2008
  4. pmc Unique scorpion toxin with a putative ancestral fold provides insight into evolution of the inhibitor cystine knot motif
    Jennifer J Smith
    Institute for Molecular Bioscience and School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia QLD 4072, Australia
    Proc Natl Acad Sci U S A 108:10478-83. 2011
  5. ncbi The structure of the potassium channel: molecular basis of K+ conduction and selectivity
    D A Doyle
    Laboratory of Molecular Neurobiology and Biophysics and the Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 280:69-77. 1998
  6. ncbi Epidemiology of scorpionism: a global appraisal
    J P Chippaux
    Institut de Recherche pour le Developpement, Unité de recherche Santé de la mère et de l enfant en milieu tropical, La Paz, Bolivia
    Acta Trop 107:71-9. 2008
  7. ncbi Were arachnids the first to use combinatorial peptide libraries?
    Brianna L Sollod
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032 3305, USA
    Peptides 26:131-9. 2005
  8. pmc Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal
    Yibao Ma
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China
    BMC Genomics 10:290. 2009
  9. ncbi Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels
    K J Swartz
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 18:675-82. 1997
  10. ncbi Cloning and characterization of cDNA sequences encoding for new venom peptides of the Brazilian scorpion Opisthacanthus cayaporum
    Edelyn C N Silva
    Departamento de Ciencias Fisiologicas, Universidade de Brasilia, Brasilia, DF 70910 900, Brazil
    Toxicon 54:252-61. 2009

Detail Information

Publications278 found, 100 shown here

  1. pmc SdPI, the first functionally characterized Kunitz-type trypsin inhibitor from scorpion venom
    Ruiming Zhao
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People s Republic of China
    PLoS ONE 6:e27548. 2011
    ..However, no Kunitz-type peptides from scorpion venom have been functionally characterized...
  2. pmc Hg1, novel peptide inhibitor specific for Kv1.3 channels from first scorpion Kunitz-type potassium channel toxin family
    Zong Yun Chen
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    J Biol Chem 287:13813-21. 2012
    ..3 channels. Their structural and functional diversity strongly suggest that Kunitz-type toxins are a new source to screen and design potential peptides for diagnosing and treating Kv1.3-mediated autoimmune diseases...
  3. ncbi Structural basis of a potent peptide inhibitor designed for Kv1.3 channel, a therapeutic target of autoimmune disease
    Song Han
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    J Biol Chem 283:19058-65. 2008
    ....
  4. pmc Unique scorpion toxin with a putative ancestral fold provides insight into evolution of the inhibitor cystine knot motif
    Jennifer J Smith
    Institute for Molecular Bioscience and School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia QLD 4072, Australia
    Proc Natl Acad Sci U S A 108:10478-83. 2011
    ..Additionally, we show that U(1)-LITX-Lw1a has potent insecticidal activity across a broad range of insect pest species, thereby providing a unique structural scaffold for bioinsecticide development...
  5. ncbi The structure of the potassium channel: molecular basis of K+ conduction and selectivity
    D A Doyle
    Laboratory of Molecular Neurobiology and Biophysics and the Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 280:69-77. 1998
    ..The architecture of the pore establishes the physical principles underlying selective K+ conduction...
  6. ncbi Epidemiology of scorpionism: a global appraisal
    J P Chippaux
    Institut de Recherche pour le Developpement, Unité de recherche Santé de la mère et de l enfant en milieu tropical, La Paz, Bolivia
    Acta Trop 107:71-9. 2008
    ..27%). Although adults are more often concerned, children experience more severe envenomations and among them, mortality is higher. Improvement of therapeutic management would reduce the lethality very significantly...
  7. ncbi Were arachnids the first to use combinatorial peptide libraries?
    Brianna L Sollod
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032 3305, USA
    Peptides 26:131-9. 2005
    ..This gene-based combinatorial peptide library strategy appears to have been first implemented by arachnids almost 400 million years ago, long before cone snails evolved a similar mechanism for generating peptide diversity...
  8. pmc Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal
    Yibao Ma
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China
    BMC Genomics 10:290. 2009
    ..However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki...
  9. ncbi Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels
    K J Swartz
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 18:675-82. 1997
    ..These results suggest that residues near the outer edges of S3 and S4 form the HaTx binding sites and are eccentrically located at least 15 A from the central pore axis on the surface of voltage-gated K+ channels...
  10. ncbi Cloning and characterization of cDNA sequences encoding for new venom peptides of the Brazilian scorpion Opisthacanthus cayaporum
    Edelyn C N Silva
    Departamento de Ciencias Fisiologicas, Universidade de Brasilia, Brasilia, DF 70910 900, Brazil
    Toxicon 54:252-61. 2009
    ....
  11. ncbi Mining on scorpion venom biodiversity
    Ricardo C Rodriguez de la Vega
    Structural and Computational Biology Genome Biology Units, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Toxicon 56:1155-61. 2010
    b>Scorpion venoms are complex mixtures of dozens or even hundreds of distinct proteins, many of which are inter-genome active elements...
  12. ncbi Molecular dissection of venom from Chinese scorpion Mesobuthus martensii: identification and characterization of four novel disulfide-bridged venom peptides
    Xian Chun Zeng
    State Key Laboratory of Virology, Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China
    Peptides 27:1745-54. 2006
    ..BmKKx2 is a new member of the gamma-K+ -channel toxin subfamily (classified as gamma-KTx 2.2). The venoms of scorpions thus continue to provide novel toxins with potential novel actions on targets...
  13. ncbi The tale of a resting gland: transcriptome of a replete venom gland from the scorpion Hottentotta judaicus
    David Morgenstern
    Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072, Australia
    Toxicon 57:695-703. 2011
    ..This study therefore provides an important foundation for future studies into the dynamics of transcription in the venom glands of scorpions and other venomous animals...
  14. ncbi Molecular diversity of toxic components from the scorpion Heterometrus petersii venom revealed by proteomic and transcriptome analysis
    Yibao Ma
    College of Life Sciences, Wuhan University, Wuhan, People s Republic of China
    Proteomics 10:2471-85. 2010
    b>Scorpion venoms contain a vast untapped reservoir of natural products, which have the potential for medicinal value in drug discovery...
  15. ncbi Current views on scorpion toxins specific for K+-channels
    Ricardo C Rodriguez de la Vega
    Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad, 2001, Apartado Postal 510 3, Cuernavaca 62210, Mexico
    Toxicon 43:865-75. 2004
    ..At the same time, several new subfamilies of specific toxins for K+-channels were isolated from scorpion venoms, enhancing the availability and diversity of such useful molecular tools...
  16. ncbi Overview of scorpion toxins specific for Na+ channels and related peptides: biodiversity, structure-function relationships and evolution
    Ricardo C Rodriguez de la Vega
    Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Av Universidad 2001, Apartado Postal 510 3, Cuernavaca Morelos 62210, Mexico
    Toxicon 46:831-44. 2005
    b>Scorpion venoms contain a large number of bioactive components...
  17. ncbi A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies
    J Tytgat
    Laboratory of Toxicology, University of Leuven, E Van Evenstraat 4, 3000 Leuven, Belgium
    Trends Pharmacol Sci 20:444-7. 1999
    ..Because of the pharmacological divergence of these peptides, the principle of classification was based on a primary sequence alignment, combined with maximum parsimony and Neighbour-Joining analysis...
  18. ncbi Nuclear magnetic resonance structural studies of a potassium channel-charybdotoxin complex
    Liping Yu
    Pharmaceutical Discovery Division, GPRD, Abbott Laboratories, Abbott Park, Illinois 60064 6098, USA
    Biochemistry 44:15834-41. 2005
    ....
  19. pmc Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier
    Omid Veiseh
    Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
    Cancer Res 69:6200-7. 2009
    ....
  20. pmc In vivo MRI detection of gliomas by chlorotoxin-conjugated superparamagnetic nanoprobes
    Conroy Sun
    Department of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USA
    Small 4:372-9. 2008
    ..High targeting specificity and benign biological response establish this nanoprobe as a potential platform to aid in the diagnosis and treatment of gliomas and other tumors of neuroectodermal origin...
  21. ncbi Molecular basis of inhibitory peptide maurotoxin recognizing Kv1.2 channel explored by ZDOCK and molecular dynamic simulations
    Hong Yi
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, People s Republic of China
    Proteins 70:844-54. 2008
    ....
  22. ncbi Mapping of scorpion toxin receptor sites at voltage-gated sodium channels
    Michael Gurevitz
    Department of Plant Molecular Biology and Ecology, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
    Toxicon 60:502-11. 2012
    ..The other point of interaction between the toxin and the channel has not yet been defined and may involve channel residues of either the Pore-module or the Gating-module...
  23. ncbi Tityus serrulatus scorpion venom and toxins: an overview
    Camila T Cologna
    Departamento de Fisica e Quimica, Faculdade de Ciências Farmacêuticas de Ribeirão Preto USP, Ribeirao Preto SP, Brazil
    Protein Pept Lett 16:920-32. 2009
    ....
  24. ncbi Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci
    Mandana Veiseh
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Cancer Res 67:6882-8. 2007
    ..Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies...
  25. pmc Modeling the binding of three toxins to the voltage-gated potassium channel (Kv1.3)
    Rong Chen
    Research School of Biology, Australian National University, Canberra, Australia
    Biophys J 101:2652-60. 2011
    ..We pinpoint the residues in the toxins and the channel that are critical for the formation of the stable venom-channel complexes...
  26. ncbi A new class of scorpion toxin binding sites related to an A-type K+ channel: pharmacological characterization and localization in rat brain
    H Vacher
    , , Marseille, France
    FEBS Lett 501:31-6. 2001
    ..A high density of 125I-sBmTX3 binding sites was found in the striatum, hippocampus, superior colliculus, and cerebellum in the adult rat brain...
  27. ncbi Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus
    Bin Gao
    Group of Animal Innate Immunity, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing, China
    Biochimie 92:350-9. 2010
    ..Killing of intraerythrocytic P. falciparum without harming mammalian cells (erythrocytes and GC-2 cell) make them attractive candidates for antimalarial drug design...
  28. pmc CysView: protein classification based on cysteine pairing patterns
    Johann Lenffer
    Institute for Infocomm Research, 21 Heng Mui Keng Terrace, 119613 Singapore
    Nucleic Acids Res 32:W350-5. 2004
    ..CysView can be used to support distant homology between proteins. CysView is publicly available at http://research.i2r.a-star.edu.sg/CysView/...
  29. pmc Structure-function map of the receptor site for β-scorpion toxins in domain II of voltage-gated sodium channels
    Joel Z Zhang
    Department of Pharmacology, University of Washington, Seattle, Washington 98195 7280, USA
    J Biol Chem 286:33641-51. 2011
    ..These results provide new molecular insights into the voltage sensor-trapping model of toxin action and further define the molecular requirements for the development of antagonists that can prevent or reverse toxicity of scorpion toxins...
  30. ncbi Utility of scorpion antivenin vs prazosin in the management of severe Mesobuthus tamulus (Indian red scorpion) envenoming at rural setting
    H S Bawaskar
    Bawaskar Hospital and Research Center, Mahad, Dist Raigad Maharashtra, India 402 301
    J Assoc Physicians India 55:14-21. 2007
    ..We compared the effects of SAV Vs Prazosin (PRA) in the management of severe scorpion stung cases at rural setting in a non-randomised open label manner...
  31. ncbi Proteomic analysis of the venom of Heterometrus longimanus (Asian black scorpion)
    Scott Bringans
    Proteomics International, Perth, Western Australia, Australia
    Proteomics 8:1081-96. 2008
    ..As a validation of this approach, the functional composition of the experimentally derived proteome is similar to that of other scorpions, and contains a potent mix of toxins, antimicrobials and ionic channel inhibitors...
  32. pmc Efficacy and safety of scorpion antivenom plus prazosin compared with prazosin alone for venomous scorpion (Mesobuthus tamulus) sting: randomised open label clinical trial
    Himmatrao Saluba Bawaskar
    Bawaskar Hospital and Research Centre, Mahad, Dist Raigad, Maharashtra, India 402301
    BMJ 342:c7136. 2011
    ..We assessed the efficacy of prazosin combined with scorpion antivenom, compared with prazosin alone, in individuals with autonomic storm caused by scorpion sting...
  33. ncbi Scorpion toxins specific for Na+-channels
    L D Possani
    Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad 2001, Cuernavaca, Mexico
    Eur J Biochem 264:287-300. 1999
    ..The presence of an intron at the DNA level, situated in the middle of the signal peptide, has been demonstrated...
  34. ncbi A hyaluronidase from Tityus serrulatus scorpion venom: isolation, characterization and inhibition by flavonoids
    A C Pessini
    , , USP, , 14040-903, SP, , Brazil
    Toxicon 39:1495-504. 2001
    ....
  35. ncbi Genetic mechanisms of scorpion venom peptide diversification
    Cao Zhijian
    State Key Laboratory of Virology, College of Life Sciences, Wuhan Uiniversity, Wuhan 430072, People s Republic of China
    Toxicon 47:348-55. 2006
    ..In order to completely clarify the diversity of scorpion toxins and toxin-like peptides, toxinomics (genomics and proteomics of scorpion toxins and toxin-like peptides) are expected to greatly advance in the near future...
  36. ncbi Interaction simulation of hERG K+ channel with its specific BeKm-1 peptide: insights into the selectivity of molecular recognition
    Hong Yi
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, People s Republic of China
    J Proteome Res 6:611-20. 2007
    ....
  37. ncbi Scorpion venom peptides without disulfide bridges
    Xian Chun Zeng
    Department of Biotechnology, Key Laboratory of MOE for Virology, Institute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P R China
    IUBMB Life 57:13-21. 2005
    ..Based on their pharmacological activities and peptide size similarity, we have classified these peptides into six subfamilies...
  38. pmc Voltage-gated sodium channel modulation by scorpion alpha-toxins
    Frank Bosmans
    Laboratory of Toxicology, University of Leuven, O and N 2, Postbus 922, Herestraat 49, 3000 Leuven, Belgium
    Toxicon 49:142-58. 2007
    ..This fascinating aspect justifies an overview on scorpion venoms, their alpha-toxins and the Na(+) channel targets they are built for, as well as on the molecular ..
  39. ncbi Scorpion and spider venom peptides: gene cloning and peptide expression
    V Quintero-Hernández
    Instituto de Biotecnologia UNAM, Avenida Universidad, Colonia Chamilpa, Cuernavaca, Morelos, Mexico
    Toxicon 58:644-63. 2011
    ....
  40. ncbi Molecular diversity of the telson and venom components from Pandinus cavimanus (Scorpionidae Latreille 1802): transcriptome, venomics and function
    Elia Diego-Garcia
    Laboratory of Toxicology, University of Leuven, Campus Gasthuisberg, Leuven, Belgium
    Proteomics 12:313-28. 2012
    ..Expressed sequence tags from venom glands provide complementary information to MS and reveal undescribed components related to the biological activity of the venom...
  41. pmc Vesicle-associated membrane protein (VAMP) cleavage by a new metalloprotease from the Brazilian scorpion Tityus serrulatus
    Paul L Fletcher
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, USA
    J Biol Chem 285:7405-16. 2010
    ..We have isolated antarease, a metalloprotease from fraction nu that cleaves VAMP2, and report its amino acid sequence...
  42. ncbi Selective blocking of voltage-gated K+ channels improves experimental autoimmune encephalomyelitis and inhibits T cell activation
    C Beeton
    Laboratoire d'Immunologie, , , , , 13385 Marseille Cedex 5, France
    J Immunol 166:936-44. 2001
    ..We conclude that KTX is a potent immunosuppressive agent with beneficial effects on the neurological symptoms of EAE...
  43. ncbi Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression
    Nancy Nutile-McMenemy
    Department of Anesthesiology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire, USA
    J Neurochem 103:2035-46. 2007
    ....
  44. ncbi An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch
    Cyril Goudet
    Laboratory of Toxicology, Faculty of Pharmaceutical Sciences, University of Leuven, Van Evenstraat 4, B 3000, Leuven, Belgium
    Toxicon 40:1239-58. 2002
    ..The work on the venom of BmK led to the discovery of two new subfamilies, alpha-KT x 14 and alpha-KT x 17...
  45. pmc Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates
    Zhenhuan Zhao
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, 430072, China
    Antimicrob Agents Chemother 53:3472-7. 2009
    ..These results revealed that imcroporin could be considered a potential anti-infective drug or lead compound, especially for treating antibiotic-resistant pathogens...
  46. ncbi The insecticidal potential of scorpion beta-toxins
    Michael Gurevitz
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Toxicon 49:473-89. 2007
    ..This information may be useful in future attempts to mimic the bioactive surface of the toxins for the design of anti-insect selective peptidomimetics...
  47. ncbi Positions under positive selection--key for selectivity and potency of scorpion alpha-toxins
    Hagar Weinberger
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel
    Mol Biol Evol 27:1025-34. 2010
    ....
  48. ncbi Peptides and genes coding for scorpion toxins that affect ion-channels
    L D Possani
    Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad, 2001, Apartado Postal 510 3, 62210, Cuernavaca, Mexico
    Biochimie 82:861-8. 2000
    ..The physiological function of these peptides is manifested by a blockage of ion passage through the channels or by a modification of the gating mechanism that controls opening and closing of the ion pore...
  49. pmc Characterization of unique amphipathic antimicrobial peptides from venom of the scorpion Pandinus imperator
    G Corzo
    Suntory Institute for Bioorganic Research, Mishima gun, Shimamoto cho, Wakayamadai 1 1 1, Osaka 618 8503, Japan
    Biochem J 359:35-45. 2001
    ..Their presence brings new insights into the mode of action of scorpion venom and also opens new avenues for the discovery of novel antibiotic molecules from arthropod venoms...
  50. ncbi Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa
    Leentje Moerman
    Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, Kortrijk, Belgium
    Eur J Biochem 269:4799-810. 2002
    ..Furthermore, all peptides inhibited growth of fungi. Experiments with SYTOX green suggested that this effect is related to membrane permeabilization...
  51. ncbi Two heteromeric Kv1 potassium channels differentially regulate action potential firing
    Paul D Dodson
    Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, LE1 9HN, United Kingdom
    J Neurosci 22:6953-61. 2002
    ..Our results show that heteromeric channels containing Kv1.2 subunits govern AP firing and suggest that their localization at the initial segment of MNTB axons can explain their dominance of AP firing behavior...
  52. ncbi The Brazilian scorpion Tityus costatus Karsch: genes, peptides and function
    Elia Diego-Garcia
    Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad 2001, Apartado Postal 510 3, Cuernavaca 62210, Mexico
    Toxicon 45:273-83. 2005
    ..Some of these peptides and genes are similar to other known scorpion toxins. Based on these results, stings by scorpions of the species Tityus costatus should be taken with caution by medical doctors...
  53. pmc Rational engineering of a miniprotein that reproduces the core of the CD4 site interacting with HIV-1 envelope glycoprotein
    C Vita
    Departement d Ingenierie et d Etudes des Proteines, Commissariat a l Energie Atomique, Saclay, 91190 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 96:13091-6. 1999
    ....
  54. ncbi Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion
    Itzel Amaro
    Department of Molecular Medicine and Bioprocesses, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Avenida Universidad, 2001, Col Chamilpa 510 3, Cuernavaca 62210, Mexico
    Immunol Lett 139:73-9. 2011
    ..Mice challenged with a LD(50) of Ts1 in the presence of this scFv were substantially resistant to intoxication. ScFv 15e is a leading compound for the development of better anti-scorpion antidotes...
  55. ncbi Analgesic-antitumor peptide inhibits proliferation and migration of SHG-44 human malignant glioma cells
    Youlong Zhao
    Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210097, Jiangsu, China
    J Cell Biochem 112:2424-34. 2011
    ..These findings suggest rAGAP inhibit proliferation and migration of SHG-44 cells by arresting cell cycle and interfering p-AKT, NF-κB, BCL-2, and MAPK signaling pathways...
  56. ncbi Extreme diversity of scorpion venom peptides and proteins revealed by transcriptomic analysis: implication for proteome evolution of scorpion venom arsenal
    Yibao Ma
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, PR China
    J Proteomics 75:1563-76. 2012
    ..Our results provide an insight into the recruitment pattern of the scorpion venom arsenal for the first time...
  57. pmc Scorpion β-toxin interference with NaV channel voltage sensor gives rise to excitatory and depressant modes
    Enrico Leipold
    Department of Biophysics, Center for Molecular Biomedicine, Friedrich Schiller University of Jena and Jena University Hospital, Jena D 07745, Germany
    J Gen Physiol 139:305-19. 2012
    ..The results are best explained by a two-state voltage-sensor trapping model in that bound scorpion β toxin slows the activation as well as the deactivation kinetics of the voltage sensor in domain 2...
  58. ncbi NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical beta-toxin sequence but with alpha-like physiological activity
    Federico Del Río-Portilla
    Institute of Chemistry, National Autonomous University of Mexico, Mexico City, Mexico
    Eur J Biochem 271:2504-16. 2004
    ....
  59. ncbi Identification and functional characterization of novel scorpion venom peptides with no disulfide bridge from Buthus martensii Karsch
    Xian Chun Zeng
    Department of Biotechnology, Institute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China
    Peptides 25:143-50. 2004
    ..Functional tests with synthetic peptide showed that BmKn2 has strong antimicrobial activity against both Gram-positive and Gram-negative bacteria, whereas BmKb1 has weak activity in inhibiting the growth of these bacteria...
  60. ncbi Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli
    Yan feng Liu
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China
    Protein Expr Purif 27:253-8. 2003
    ..The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice...
  61. ncbi Disulfide bridges and blockage of Shaker B K(+)-channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus
    Fredy V Coronas
    Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico
    Toxicon 41:173-9. 2003
    ..It blocks in a completely reversible manner the Shaker B K(+)-channels, with a K(d) around 660nM. It belongs to the sub-family 12 and it is now being classified as alpha-KTx 12.2...
  62. ncbi Interaction of agitoxin2, charybdotoxin, and iberiotoxin with potassium channels: selectivity between voltage-gated and Maxi-K channels
    Ying Duo Gao
    Molecular Systems, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    Proteins 52:146-54. 2003
    ..This study also suggests that the extra net negative charge of IbTX is not related to the selectivity of this peptide for the Maxi-K channel...
  63. pmc Mapping the interaction site for a β-scorpion toxin in the pore module of domain III of voltage-gated Na(+) channels
    Joel Z Zhang
    Department of Pharmacology, University of Washington, Seattle, WA 98195 7280, USA
    J Biol Chem 287:30719-28. 2012
    ..Binding of α- and β-scorpion toxins to two distinct, pseudo-symmetrically organized receptor sites on Na(V) channels acts synergistically to modify channel gating and paralyze prey...
  64. ncbi Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes
    Mohamed A Abdel-Rahman
    Zoology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt
    Toxicon 53:349-59. 2009
    ..Taken together, we suspect that a combination of local environmental conditions, geographical separation and genetic separation may play a major role in the intraspecific variation of venom of S. m. palmatus...
  65. ncbi Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans
    Gina D'suze
    Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Biochimie 91:1010-9. 2009
    ..The N-terminal amino acid sequence of several peptides is reported here for the first time. A phylogenetic tree analysis is also reported, as well as three three-dimensional models of representative toxins...
  66. pmc Molecular requirements for recognition of brain voltage-gated sodium channels by scorpion alpha-toxins
    Roy Kahn
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    J Biol Chem 284:20684-91. 2009
    ..5 (cardiac), and rNa(v)1.6 channels, with no hindrance of its activity at Na(v)1.1-1.3. These results suggest that by employing a similar approach the design of further target-selective sodium channel modifiers is imminent...
  67. pmc U-shaped dose-dependent effects of BmK AS, a unique scorpion polypeptide toxin, on voltage-gated sodium channels
    Mang Mang Zhu
    School of Life Sciences, Shanghai University, Shanghai, China
    Br J Pharmacol 158:1895-903. 2009
    ..Buthus martensi Karsch (BmK) AS is a scorpion polypeptide toxin, said to target the voltage-gated sodium channels (VGSCs). However, the mechanism of action of BmK AS on the VGSCs has yet to be defined...
  68. ncbi Cloning and functional characterization of a new antimicrobial peptide gene StCT1 from the venom of the scorpion Scorpiops tibetanus
    Wenying Yuan
    Hubei Maternal and Child Health Hospital, Wuhan, China
    Peptides 31:22-6. 2010
    ..These results show that the antimicrobial peptide encoded by StCT1 gene from the venom of the scorpion S. tibetanus is a potential anti-infective polypeptide or lead compound, especially for treating antibiotics-resistant pathogens...
  69. ncbi Molecular determination of selectivity of the site 3 modulator (BmK I) to sodium channels in the CNS: a clue to the importance of Nav1.6 in BmK I-induced neuronal hyperexcitability
    Huiqiong He
    Graduate School of Chinese Academy of Sciences, Shanghai Institute of Physiology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China
    Biochem J 431:289-98. 2010
    ....
  70. ncbi Tityus serrulatus Hypotensins: a new family of peptides from scorpion venom
    T Verano-Braga
    Departamento de Bioquimica e Imunologia, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, 31270 901 Pampulha, Belo Horizonte, Brazil
    Biochem Biophys Res Commun 371:515-20. 2008
    ..Both TsHpt could not inhibit ACE activity. These peptides appear to exert their anti-hypertensive effect through NO-dependent and ACE-independent mechanisms...
  71. ncbi Expression and purification of an antitumor-analgesic peptide from the venom of Mesobuthus martensii Karsch by small ubiquitin-related modifier fusion in Escherichia coli
    Peng Cao
    Laboratory of Cellular and Molecular Biology, Jiangsu Province Institute of Traditional Chinese Medicine, Nanjing 210028, China
    Biotechnol Prog 26:1240-4. 2010
    ..The further writhing experiment showed that the rAGAP had an intensive analgesic effect. The expression strategy presented in this study allows convenient high yield and easy purification of the rAGAP with native sequences...
  72. ncbi Degranulation of mast cells and histamine release involved in rat pain-related behaviors and edema induced by scorpion Buthus martensi Karch venom
    Tong Liu
    Graduate School of the Chinese Academy of Sciences, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China
    Eur J Pharmacol 575:46-56. 2007
    ..Thus, these results suggest that the peripheral cellular incidents of mast cells degranulation and histamine release are involved in BmK venom-induced pain-related behaviors and inflammation...
  73. pmc Exploiting cross-reactivity to neutralize two different scorpion venoms with one single chain antibody fragment
    Lidia Riaño-Umbarila
    Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apartado Postal 510 3, Cuernavaca, Morelos 62250, Mexico
    J Biol Chem 286:6143-51. 2011
    We report the optimization of a family of human single chain antibody fragments (scFv) for neutralizing two scorpion venoms. The parental scFv 3F recognizes the main toxins of Centruroides noxius Hoffmann (Cn2) and Centruroides suffusus ..
  74. pmc Alpha-scorpion toxin impairs a conformational change that leads to fast inactivation of muscle sodium channels
    Fabiana V Campos
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    J Gen Physiol 132:251-63. 2008
    ..These results are consistent with the proposed model, in which Ts3 specifically impairs the fraction of the movement of the S4-DIV that allows fast inactivation to occur at normal rates...
  75. ncbi A maurotoxin with constrained standard disulfide bridging: innovative strategy of chemical synthesis, pharmacology, and docking on K+ channels
    Sarrah M'Barek
    Laboratoire International Associé d Ingénierie Biomoléculaire, CNRS Unité Mixte de Recherche 6560 Bd Pierre Dramard, 13916 Marseille Cedex 20, France
    J Biol Chem 278:31095-104. 2003
    ..2, and Kv1.3) were found to correlate with MTXPi1 pharmacology. A functional map detailing the interaction between MTXPi1 and Shaker B channel was generated in line with docking experiments...
  76. pmc BeKm-1 is a HERG-specific toxin that shares the structure with ChTx but the mechanism of action with ErgTx1
    Mei Zhang
    Department of Physiology, Virginia Commonwealth University, Richmond 23298, USA
    Biophys J 84:3022-36. 2003
    ..Toxin foot printing using BeKm-1 and ErgTx1 will likely provide complementary information about the unique outer vestibule structure of the HERG channel...
  77. pmc A designer ligand specific for Kv1.3 channels from a scorpion neurotoxin-based library
    Zoltan Takacs
    Department of Pediatrics and Institute of Molecular Pediatric Sciences, Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 106:22211-6. 2009
    ..This scaffold-based/target-biased strategy overcomes many obstacles to production of selective toxins...
  78. pmc Developing a comparative docking protocol for the prediction of peptide selectivity profiles: investigation of potassium channel toxins
    Po Chia Chen
    School of Physics, Building A28, University of Sydney, NSW 2006, Australia
    Toxins (Basel) 4:110-38. 2012
    ....
  79. ncbi Mapping of maurotoxin binding sites on hKv1.2, hKv1.3, and hIKCa1 channels
    Violeta Visan
    Department of Applied Physiology, University of Ulm, Albert Einstein Allee 11, D 89081 Ulm, Germany
    Mol Pharmacol 66:1103-12. 2004
    ..A negatively charged amino acid in this position could better stabilize the toxin-channel interaction and could explain the pH(o) sensitivity of MTX block on current through hIKCa1 versus hKv1.2 channels...
  80. pmc Kv1.3 channels in postganglionic sympathetic neurons: expression, function, and modulation
    Megan A Doczi
    Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA
    Am J Physiol Regul Integr Comp Physiol 295:R733-40. 2008
    ..3 influences sympathetic control of cardiovascular function. Our data also indicate that modulation of Kv1.3 is likely to affect sympathetic control of cardiovascular function...
  81. ncbi The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae
    Yousra Abdel-Mottaleb
    Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, P O Box 922, 3000 Leuven, Belgium
    FEBS Lett 580:6254-8. 2006
    ..1-Kv1.5 and Shaker IR) expressed in Xenopus laevis oocytes. Interestingly, OdK1 selectively inhibited the currents through Kv1.2 channels with an IC50 value of 183+/-3 nM but did not affect any of the other channels...
  82. ncbi BmP09, a "long chain" scorpion peptide blocker of BK channels
    Jing Yao
    Institute of Biochemistry and Biophysics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
    J Biol Chem 280:14819-28. 2005
    ..The stable binding between the toxin BmP09 and the BK channel is favored by aromatic pi -pi interactions around the center...
  83. ncbi Engineering a potent and specific blocker of voltage-gated potassium channel Kv1.3, a target for autoimmune diseases
    Rong Chen
    Research School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Biochemistry 51:1976-82. 2012
    ..We show that the IC(50) values predicted for three toxins and three channels match closely with experiment. Kv1.3 is half-blocked by 0.2 pM mutant OSK1; it is >10000-fold more specific for this channel than for Kv1.1 and Kv1.2...
  84. ncbi Protein-protein recognition control by modulating electrostatic interactions
    Song Han
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, P R China
    J Proteome Res 9:3118-25. 2010
    ..The novel function findings of BmP05-T peptide suggested that the control recognition technique described here had the potential for use in designing and utilizing functional proteins in many biological scenarios...
  85. ncbi Expression and characterization of recombinant kurtoxin, an inhibitor of T-type voltage-gated calcium channels
    Chul Won Lee
    Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Republic of Korea
    Biochem Biophys Res Commun 416:277-82. 2011
    ....
  86. ncbi A potent potassium channel blocker from Mesobuthus eupeus scorpion venom
    Bin Gao
    Group of Animal Innate Immunity, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China
    Biochimie 92:1847-53. 2010
    ..3, an attractive target for T-cell mediated autoimmune diseases...
  87. ncbi A subfamily of acidic alpha-K(+) toxins
    Isabelle Huys
    Laboratory of Toxicology, University of Leuven, E Van Evenstraat 4, 3000 Leuven, Belgium
    J Biol Chem 279:2781-9. 2004
    ..Analysis of rPBTx1 T24F/V26K displaying an affinity toward Kv1.2 and Kv1.3 channels in the nanomolar range shows the importance of the functional dyad above the acidic character of this toxin...
  88. pmc Brownian dynamics simulations of the recognition of the scorpion toxin maurotoxin with the voltage-gated potassium ion channels
    Wei Fu
    Center for Drug Discovery and Design, State Key Laboratory of Drug Research, Shanghai Institute of Meteria Medica, Chinese Academy of Sciences, 194 Taiyuan Road, Shanghai 200031, P R China
    Biophys J 83:2370-85. 2002
    ..This phenomenon has not been appreciated in the previous mutagenesis experiments, indicating this might be a new clue for additional functional study of Kv1 channels...
  89. ncbi Relationship between plasmatic levels of various cytokines, tumour necrosis factor, enzymes, glucose and venom concentration following Tityus scorpion sting
    G D'suze
    Lab de Neurofarmacología Celular, Biochemistry and Biophysics Center, Instituto Venezolano de Investigaciones Cientificas IVIC, Apdo 21827, Caracas 1020 A, Venezuela
    Toxicon 41:367-75. 2003
    ..Interleukin 6 (IL6) increased significantly in patients with MS symptoms. IL6 values increased with hyperamylasemia, envenoming severity and time hyperamylasemia...
  90. ncbi Maurotoxin and the Kv1.1 channel: voltage-dependent binding upon enantiomerization of the scorpion toxin disulfide bridge Cys31-Cys34
    C Lecomte
    Laboratoire de Biochimie, CNRS UMR 6560, IFR Jean Roche, Faculte de Medecine Nord, Marseille, France
    J Pept Res 55:246-54. 2000
    ..3, and to a lesser extent, Kv1.2 channels and it may contribute to the stabilization and strength of the interaction between the toxin and the Kv1.1 channel...
  91. ncbi BmKCT toxin inhibits glioma proliferation and tumor metastasis
    Shaozhong Fan
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, PR China
    Cancer Lett 291:158-66. 2010
    ..5-conjugated BmKCT revealed that BmKCT selectively targeted the glioma in situ. Our data suggest that BmKCT could be exploited as a potential therapeutic for glioma diagnosis and therapy...
  92. ncbi The new kappa-KTx 2.5 from the scorpion Opisthacanthus cayaporum
    Thalita Soares Camargos
    Laboratorio de Toxinologia, Universidade de Brasilia, Brasilia, DF 70910 900, Brazil
    Peptides 32:1509-17. 2011
    ..5-Kv1.2 complex...
  93. ncbi Isolation, molecular cloning and functional characterization of a novel beta-toxin from the Venezuelan scorpion, Tityus zulianus
    Adolfo Borges
    Sección de Biomembranas, Instituto de Medicina Experimental, Facultad de Medicina, Universidad Central de Venezuela, Box 50587, Sabana Grande, Caracas 1051, Venezuela
    Toxicon 43:671-84. 2004
    ..A comparison of Tz1 with Tityus serrulatus beta-toxin Ts1 revealed that some of the non-conservative replacements in Tz1 lie in regions potentially involved in receptor recognition...
  94. pmc Brownian dynamics simulations of interaction between scorpion toxin Lq2 and potassium ion channel
    M Cui
    Center for Drug Discovery and Design, State Key Laboratory of New Drug Research, Shanghai Institute of Meteria Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, Peoples Republic of China
    Biophys J 80:1659-69. 2001
    ....
  95. pmc Kv1.3 channel gene-targeted deletion produces "Super-Smeller Mice" with altered glomeruli, interacting scaffolding proteins, and biophysics
    D A Fadool
    Department of Biological Science, Programs in Neuroscience and Molecular Biophysics, The Florida State University, Tallahassee, FL 32306, USA
    Neuron 41:389-404. 2004
    ..3 plays a far more reaching role in signal transduction, development, and olfactory coding than that of the classically defined role of a potassium channel-to shape excitability by influencing membrane potential...
  96. ncbi Molecular basis of alpha-KTx specificity
    Kathleen M Giangiacomo
    Department of Biochemistry, Temple University School of Medicine, 3420 N Broad Street, Philadelphia, PA 19140, USA
    Toxicon 43:877-86. 2004
    ..Structure-based homology models of potassium channels complexed with alpha-KTxs, in combination with measurements of toxin binding free energy, will further our understanding of the molecular basis of alpha-KTx specificity...
  97. pmc Mucroporin, the first cationic host defense peptide from the venom of Lychas mucronatus
    Chao Dai
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, China
    Antimicrob Agents Chemother 52:3967-72. 2008
    ..These results showed that mucroporin could be considered a potential anti-infective drug, especially for treating antibiotic-resistant pathogens...
  98. ncbi Novel paradigms on scorpion toxins that affects the activating mechanism of sodium channels
    Ricardo C Rodríguez de la Vega
    Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Av Universidad 2001, Cuernavaca Morelos 62210, Mexico
    Toxicon 49:171-80. 2007
    ..Finally, the recent advances on the knowledge of the specific interactions of beta-toxins and different sub types of Na(+) channels are also reviewed...
  99. pmc Modeling the structure of agitoxin in complex with the Shaker K+ channel: a computational approach based on experimental distance restraints extracted from thermodynamic mutant cycles
    Mats A L Eriksson
    Weill Medical College of Cornell University, Department of Biochemistry, 1300 York Avenue, New York, NY 10021, USA
    Biophys J 83:2595-609. 2002
    ..Specific site-directed mutagenesis experiments are suggested to verify and confirm the structure of the toxin-channel complex...
  100. ncbi An 'Old World' scorpion beta-toxin that recognizes both insect and mammalian sodium channels
    Dalia Gordon
    Department of Plant Sciences, George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
    Eur J Biochem 270:2663-70. 2003
    ....
  101. ncbi Novel alpha-KTx peptides from the venom of the scorpion Centruroides elegans selectively blockade Kv1.3 over IKCa1 K+ channels of T cells
    Timoteo Olamendi-Portugal
    Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad, 2001, Apartado Postal 510 3, Cuernavaca 62210, Mexico
    Toxicon 46:418-29. 2005
    ..3 over IKCa1 may have significance in the development of novel tools for suppressing the function of those T cell subsets whose proliferation critically depends on the activity of Kv1.3 channels...

Research Grants28

  1. CHLORIDE CHANNELS: NOVEL TARGETS FOR CANCER TREATMENT
    Susan Lyons; Fiscal Year: 2000
    ..There is a need to increase the time to progression, limit recurrence, externl survival, and improve the quality of life through the development of highly specific, targeted, efficacious treatments. ..
  2. A peptide inhibitor: new probe for chloride channels
    NAEL MCCARTY; Fiscal Year: 2005
    ..abstract_text> ..
  3. TRAINING IN MOLECULAR NEUROBIOLOGY
    William Catterall; Fiscal Year: 2008
    ..The training program will integrate the substantial expertise of this group of faculty in a coordinated pre-doctoral and post-doctoral training effort. ..
  4. Mast cell toll-like receptors and parasitic pathogens
    MARCUS ANDRADE; Fiscal Year: 2008
    ..For aim 3, normal, mast cell-deficient and TLR-deficient mice will be used to evaluate host responses to L.sp. and T.C. infection using experimental models that are well established in our institution. [unreadable] [unreadable]..
  5. TOXIN: NA-CHANNEL INTERACTIONS AND DRUG DESIGN
    Kenneth Blumenthal; Fiscal Year: 2003
    ....
  6. Rational Design of Inhibitors of Polyamine Synthesis
    Steven Ealick; Fiscal Year: 2007
    ..An external committee with expertise in clinical oncology will advise us on project priorities and potential clinical trials. ..
  7. Activity Dependent Signaling in Muscle Fiber Plasticity
    Martin Schneider; Fiscal Year: 2007
    ..The studies proposed here should have important bearing on the effects of use and disuse on muscle fiber gene expression. [unreadable] [unreadable]..
  8. K+ CHANNEL MODEL FOR TRINUCLEOTIDE-EXPANSION DISEASES
    George Chandy; Fiscal Year: 2002
    ....
  9. Kv1.3 channels: functional biomarker and therapeutic target for Type-1 Diabetes
    George Chandy; Fiscal Year: 2006
    ..This experiment has important implications if Kv1.3-specific blockers are to be developed as therapeutics. [unreadable] [unreadable] [unreadable]..
  10. Post-transcriptional Regulation Requires Phosphorylation of HuR in Glioma
    LOUIS NABORS; Fiscal Year: 2009
    ..at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  11. SYNTHETIC PEPTIDE VACCINES FOR DENTAL CARIES
    Martin Taubman; Fiscal Year: 2005
    ..abstract_text> ..
  12. Analysis of Folate Receptor 4 Function in the Mouse
    J Salbaum; Fiscal Year: 2006
    ..Together, these studies will improve our understanding of the basic biology of folate receptor genes, with implications for the prevention of human birth defects. ..
  13. Fluorescence 2D Difference Gel Electrophoresis
    Kenneth Blumenthal; Fiscal Year: 2003
    ..abstract_text> ..
  14. Local Ca2+ signaling in sympathetic ganglion neurons
    Martin Schneider; Fiscal Year: 2005
    ..Our results will provide new insights regarding local Ca2+ signaling mechanisms that could be compromised in neuronal disease. ..
  15. Conference on Muscle: Excitation-Contraction Coupling
    Martin Schneider; Fiscal Year: 2003
    ..The organizing committee and session leaders will strive to achieve appropriate representation of women, minorities and disabled. All facilities are handicapped accessible. ..
  16. Endpoint Analyses of Anti-Integrin Therapy for Gliomas
    LOUIS NABORS; Fiscal Year: 2004
    ..Data from these studies will be critically important in developing, refining and validating non-invasive methodologies for timely assessment of specific anti-angiogenic therapies for malignant brain tumors in patients. ..
  17. THERAPEUTIC STUDIES OF ANAPLASTIC GLIOMAS
    LOUIS NABORS; Fiscal Year: 2008
    ..This proposal thus builds on our extensive clinical investigative expertise within the context of a well-established multi-institutional brain tumor treatment consortium. ..
  18. Kv1.3 channels: functional biomarker and therapeutic target for Type-1 Diabetes
    George Chandy; Fiscal Year: 2007
    ..This experiment has important implications if Kv1.3-specific blockers are to be developed as therapeutics. [unreadable] [unreadable] [unreadable]..
  19. CONTROL OF CALCIUM RELEASE IN SKELETAL MUSCLE FIBERS
    Martin Schneider; Fiscal Year: 2006
    ..The results of these studies should provide further insight into muscle pathological states and to the overall wellness associated with functioning muscle. ..
  20. Cardiac Channels: Targets of Drugs that Affect Kinetics
    Dorothy A Hanck; Fiscal Year: 2010
    ..Our strategy combines mutagenesis, whole cell, single channel, and gating current electrophysiology, and molecular modeling. ..
  21. MOLECULAR BASIS FOR Kv CHANNEL HETEROGENEITY IN THE HEART
    Gea Ny Tseng; Fiscal Year: 2010
    ..These models will be useful in structure-based design of IKs activators that can combat acquired &congenital LQT syndromes. ..
  22. STRUCTURE-FUNCTION OF THE CARDIAC SODIUM CHANNEL
    DOROTHY HANCK; Fiscal Year: 2008
    ..abstract_text> ..
  23. MULTIVALENT LIGANDS FOR NICOTINIC RECEPTORS
    Richard Fitch; Fiscal Year: 2007
    ..If successful, these studies will form the basis for a general approach to the three-dimensional (3D) crystallization of membrane proteins for x-ray structural analysis. [unreadable] [unreadable]..
  24. K CHANNELS: STRUCTURE-FUNCTION RELATION AND MODULATION
    Gea Ny Tseng; Fiscal Year: 2006
    ..This structural information will be useful for the design and development of therapeutic agents targeting HERG and KCNE. ..
  25. STRUCTURAL BASES OF T-TYPE CALCIUM CHANNEL FUNCTION
    DOROTHY HANCK; Fiscal Year: 2003
    ..These experiments will use techniques of electrophysiology, mutagenesis and molecular modeling. ..
  26. FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
    ROGER YOUNG; Fiscal Year: 2003
    ....
  27. Drug therapy targeted to the voltage-gated sodium channel
    Michael F Sheets; Fiscal Year: 2010
    ..In pursuance of this hypothesis we will determine the residues at the bundle crossing by crosslinking Cys substitutions between S6 segments. ..