Genomes and Genes
Summary: A mammalian pancreatic extract composed of enzymes with protease, amylase and lipase activities. It is used as a digestant in pancreatic malfunction.
Publications220 found, 100 shown here
- Mismatch of duodenal deliveries of dietary fat and pancreatin from enterically coated microspheresJ H Meyer
Department of Medicine, Sepulveda VA Medical Center, California, USA
Pancreas 15:226-35. 1997..We speculated that the similar transits of the 1.2-mm Creon and the 2-mm Pancrease resulted from aggregation of these microspheres in the presence of free oil...
- Pancreatin therapy in patients with insulin-treated diabetes mellitus and exocrine pancreatic insufficiency according to low fecal elastase 1 concentrations. Results of a prospective multi-centre trialNils Ewald
Third Medical Department and Policlinic, Giessen University Hospital, Giessen, Germany
Diabetes Metab Res Rev 23:386-91. 2007..This investigation was designed to evaluate the impact of enzyme-replacement therapy on glucose metabolism and diabetes treatment in a prospective study of insulin-treated patients with diabetes mellitus...
- A double-blind, randomized, multicentre, crossover study to prove equivalence of pancreatin minimicrospheres versus microspheres in exocrine pancreatic insufficiencyU Halm
Department of Internal Medicine II, University of Leipzig, Germany
Aliment Pharmacol Ther 13:951-7. 1999Modern pancreatin preparations consist of enteric-coated microspheres to protect the enzymes from gastric acid...
- A 2-year post-authorization safety study of high-strength pancreatic enzyme replacement therapy (pancreatin 40,000) in cystic fibrosisJames M Littlewood
UK Cystic Fibrosis Trust, 11 London Road, Bromley, Kent BR1 1BY, UK
Expert Opin Drug Saf 10:197-203. 2011....
- Prognostic factors in canine exocrine pancreatic insufficiency: prolonged survival is likely if clinical remission is achievedDaniel J Batchelor
Small Animal Hospital, Department of Veterinary Clinical Science, University of Liverpool, UK
J Vet Intern Med 21:54-60. 2007..Response to therapy in canine exocrine pancreatic insufficiency (EPI) varies considerably, making it difficult to determine prognosis for individual patients...
- Angiotensin I converting enzyme inhibitory peptides from in vitro pepsin-pancreatin digestion of soy proteinWendy M Y Lo
The University of British Columbia, Faculty of Agricultural Sciences, Food Science Building, 6650 N W Marine Drive, Vancouver, British Columbia V6T 1Z4, Canada
J Agric Food Chem 53:3369-76. 2005..ACE) inhibitory activity was determined in the soy protein isolate (SPI) digest produced by in vitro pepsin-pancreatin sequential digestion...
- High-gradient magnetic affinity separation of trypsin from porcine pancreatinJürgen J Hubbuch
Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800, Kgs Lyngby, Denmark
Biotechnol Bioeng 79:301-13. 2002..In small-scale studies approximately 95% of the endogenous trypsin present in a crude porcine pancreatin feedstock was recovered with a purification factor of approximately 4...
- Preparation and in vivo efficacy study of pancreatin microparticles as an enzyme replacement therapy for pancreatitisSonali Raosaheb Naikwade
C U Shah College of Pharmacy, S N D T Women s University, Santacruz W, Mumbai, Maharashtra, India
Drug Dev Ind Pharm 35:417-32. 2009..b>Pancreatin microspheres were prepared by emulsification phase separation by nonsolvent addition and solvent evaporation ..
- Properties of different pancreatin preparations used in pancreatic exocrine insufficiencyJohannes Matthias Löhr
Gastrocentrum, Department of Surgical Gastroenterology, Karolinska Institute University Hospital, Huddinge, SE 141 86 Stockholm, Sweden
Eur J Gastroenterol Hepatol 21:1024-31. 2009..Pancreatic enzyme preparations, generically called pancreatin, are not alike. Rather, they present a broad variety of pancreatin composition.
- Iron availability from iron-fortified spirulina by an in vitro digestion/Caco-2 cell culture modelG Puyfoulhoux
, , , Montpellier, France
J Agric Food Chem 49:1625-9. 2001..5-fold increase appeared using spirulina digest in comparison with meat. In view of this observed high iron availability from spirulina, we conclude that spirulina could represent an adequate source of iron...
- Esters of 3-chloro-1,2-propanediol (3-MCPD) in vegetable oils: significance in the formation of 3-MCPDW Seefelder
Quality and Safety Assurance Department, Nestle Research Center, Nestec Ltd, CH 1000 Lausanne 26, Switzerland
Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:391-400. 2008..Further work on the bioavailability, metabolism and possible toxicity of chloroesters per se is warranted...
- Fate of swallowed interleukin 8 and TNF alpha, and effect on the Wistar rat gutG L Briars
Department of Gastroenterology and Nutrition, Royal Children s Hospital Brisbane, Herston, Queensland 4029, Australia
J Cyst Fibros 1:94-8. 2002..We serially immuno-assayed IL-8 and TNF alpha solutions co-incubated with each of three pancreatin preparations at pH 4.5 and pH 8...
- In vitro gastrointestinal digestion study of pomegranate juice phenolic compounds, anthocyanins, and vitamin CAntonio Pérez-Vicente
Laboratorio Fitoquímica, Departamento Ciencia y Tecnología de los Alimentos, CEBAS CSIC, Apartado 4195, 30080 Murcia, Spain
J Agric Food Chem 50:2308-12. 2002..Nevertheless, due to pH, anthocyanins are largely transformed into non-red forms and/or degraded (97%), and similar results are obtained for vitamin C (>95% degradation)...
- In vitro study on digestion of peptides in Emmental cheese: analytical evaluation and influence on angiotensin I converting enzyme inhibitory peptidesSandrine Parrot
Laboratoire de Recherche en Génie Industriel Alimentaire, Rue Henri de Boissieu, F 01060 Bourg en Bresse, France
Nahrung 47:87-94. 2003..This protocol consisted in a 30 min incubation with pepsin followed by a 4 h incubation with trypsin or pancreatin. It was applied to an Emmental cheese water-soluble extract (WSE) and to a casein solution (as a control)...
- Antihypertensive properties of spinach leaf protein digestsYanjun Yang
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Agric Food Chem 52:2223-5. 2004..Pepsin and pepsin-pancreatin digests of spinach leaf protein have potent angiotensin-I converting enzyme inhibitory properties with IC(50) ..
- Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scaleNoriko Higaki-Sato
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Shimogamo, Japan
J Agric Food Chem 51:8-13. 2003An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide...
- Chitosan-alginate multilayer beads for gastric passage and controlled intestinal release of proteinAnil K Anal
Bioprocess Technology Program, Asian Institute of Technology, Klong Luang, Bangkok, Thailand
Drug Dev Ind Pharm 29:713-24. 2003..The multilayer beads disintegrated very slowly. The enzymes pepsin and pancreatin did not change the characteristics of BSA-loaded chitosan-alginate beads...
- Stability of recombinant human alpha-1-antitrypsin produced in rice in infant formulaWinyoo Chowanadisai
Department of Nutrition, University of California, Davis 95616, USA
J Nutr Biochem 14:386-93. 2003..This study shows that recombinant AAT may survive the conditions of the infant stomach and duodenum and affect protein digestion in the infant small intestine...
- Spasmocanulase in irritable bowel syndromeAbdelrahman E Mohamed
Department of Gastroenterology, Armed Forces Hospital, PO Box 7897, Riyadh 11159, Kingdom of Saudi Arabia
Saudi Med J 23:1074-7. 2002..Spasmocanulase, which has an anti-spasmodic and anti-flatulence effect and contains several ingredients, was tried in patients with irritable bowel syndrome, who had been on other medications previously without improvement...
- Estimation of arsenic bioaccessibility in edible seaweed by an in vitro digestion methodJ M Laparra
Institute of Agrochemistry and Food Technology CSIC, Aptdo 73, 46100, Burjassot Valencia, Spain
J Agric Food Chem 51:6080-5. 2003..An in vitro gastrointestinal digestion (pepsin, pH 2, and pancreatin-bile extract, pH 7) was applied to obtain the mineral soluble fraction of three seaweeds (Hizikia fusiforme, ..
- Alpha(1)-antitrypsin and antichymotrypsin in human milk: origin, concentrations, and stabilityWinyoo Chowanadisai
Department of Nutrition, The University of California, Davis 95616, USA
Am J Clin Nutr 76:828-33. 2002..It has been hypothesized that alpha(1)-antitrypsin and antichymotrypsin may modulate digestion in the infant gut...
- In vitro gastrointestinal digestion study of broccoli inflorescence phenolic compounds, glucosinolates, and vitamin CFernando Vallejo
Departamento de Ciencia y Tecnologia de los Alimentos, CEBAS CSIC, PO Box 164, 30100 Espinardo, Murcia, Spain
J Agric Food Chem 52:135-8. 2004..Thus, glucosinolates were mainly degraded by gastric conditions, whereas phenolic compounds and vitamin C were degraded by intestinal conditions...
- The effect of pH, time and dietary source of cadmium on the bioaccessibility and adsorption of cadmium to/from lettuce (Lactuca sativa L. cv. Ostinata)M Waisberg
Department of Land Resource Science, University of Guelph, Guelph, Ontario, Canada
Food Chem Toxicol 42:835-42. 2004..Therefore we suggest that part of the reduction in bioaccessibility following intestinal digestion could be explained by an increase in adsorption of metal to the plant material at higher pH values...
- Interactions of different phenolic acids and flavonoids with soy proteinsHarshadrai M Rawel
Institute of Nutritional Science, University of Potsdam, A Scheunert Allee 114 116, D 14558 Bergholz Rehbruecke, Germany
Int J Biol Macromol 30:137-50. 2002..The influence of derivatization on the in-vitro digestibility with trypsin, chymotrypsin, pepsin and pancreatin was also assessed...
- Purification of an ACE inhibitory peptide after hydrolysis of sunflower (Helianthus annuus L.) protein isolatesCristina Megías
Instituto de la Grasa, Padre Garcia Tejero 4, 41012 Sevilla, Spain
J Agric Food Chem 52:1928-32. 2004Sunflower protein isolates and the proteases pepsin and pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE)...
- Chitosan/beta-lactoglobulin core-shell nanoparticles as nutraceutical carriersLingyun Chen
Chaire de recherche du Canada sur les proteines, Institut de recherche sur les nutraceutiques et les aliments fonctionnels INAF STELA, Universite Laval, Pavillon Paul Comtois, Sainte Foy, Que, Canada
Biomaterials 26:6041-53. 2005..When transferred to simulated intestinal conditions, the (beta)lg shells of the nanoparticles were degraded by pancreatin.
- Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach RubiscoYanjun Yang
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Agric Food Chem 51:4897-902. 2003..angiotensin I-converting enzyme (ACE), that is, MRWRD, MRW, LRIPVA, and IAYKPAG, were isolated from the pepsin-pancreatin digest of spinach Rubisco with the use of HPLC. IC(50) values of individual peptides were 2.1, 0.6, 0.38, and 4...
- Phenanthrene release from natural organic matter surrogates under simulated human gastrointestinal conditionsChristopher M Hurdzan
Soil Science Program, Ohio State University, Columbus, OH 43210, USA
Ecotoxicol Environ Saf 69:525-30. 2008..These data demonstrate that the fractions of NOM retained phenanthrene to a varying extent and thus the predictions of phenanthrene bioavailability should also be assessed on the basis of the constituents of the NOM matrix...
- Effects of block length on the enzymatic degradation and erosion of oxazoline linked poly-epsilon-caprolactoneMika Pulkkinen
Department of Pharmaceutics, University of Kuopio, P O Box 1627, FIN 70211 Kuopio, Finland
Eur J Pharm Sci 31:119-28. 2007..Solvent cast PCL and PCL-O films were incubated (22 days) in the presence of pancreatin (1%, pH 7.5), with and without enzyme inhibitors...
- Structural development of self nano emulsifying drug delivery systems (SNEDDS) during in vitro lipid digestion monitored by small-angle X-ray scatteringDimitrios G Fatouros
Department of Pharmaceutics and Analytical Chemistry, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, 2100, Copenhagen, Denmark
Pharm Res 24:1844-53. 2007..To investigate the structural development of the colloid phases generated during lipolysis of a lipid-based formulation in an in vitro lipolysis model, which simulates digestion in the small intestine...
- A lipid-based liquid crystalline matrix that provides sustained release and enhanced oral bioavailability for a model poorly water soluble drug in ratsBen J Boyd
Department of Pharmaceutics, Victorian College of Pharmacy, Monash University, 381 Royal Pde, Parkville, VIC 3052, Australia
Int J Pharm 340:52-60. 2007..The results indicate that lipids that form liquid crystalline structures in excess water, may have application as an oral sustained release delivery system, providing they are not digested rapidly on administration...
- Ruminal and intestinal degradability of distillers grains plus solubles varies by sourceD H Kleinschmit
Dairy Science Department, South Dakota State University, Brookings 57007, USA
J Dairy Sci 90:2909-18. 2007..Feeds were incubated in the rumen for 3, 6, 12, 18, 24, and 36 h. Intestinal CP digestibility via pepsin and pancreatin and AA profiles were measured on residue from 12-h ruminal incubation of feeds...
- Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb)Jin Boo Jeong
School of Bioresources Sciences, Andong National University, Korea
J Agric Food Chem 55:10707-13. 2007..assay for measuring bioavailability was carried out on SNL crude protein and autoclaved SNL using pepsin and pancreatin. A nonradioactive histone acetyltransferase (HAT) assay and HAT activity colorimetric assay were used to measure ..
- Pancreatic enzyme pharmacotherapyMarcus Ferrone
Department of Clinical Pharmacy, Drug Product Services Laboratory, University of California, San Francisco, California 94118 0613, USA
Pharmacotherapy 27:910-20. 2007..Despite the large number of specialty enzyme replacements available commercially, many patients remain dissatisfied with standard therapy, and future developments are needed to optimize treatment in these individuals...
- In vitro assessment of the gastrointestinal transit tolerance of taxonomic reference strains from human origin and probiotic product isolates of BifidobacteriumL Masco
Laboratory of Microbiology, Ghent University, K L Ledeganckstraat 35, B 9000 Gent, Belgium
J Dairy Sci 90:3572-8. 2007..the bifidobacterial strains used in this study appeared to possess a natural ability to survive the presence of pancreatin (pH 8.0)...
- Improvement of digestibility, reduction in allergenicity, and induction of oral tolerance of wheat gliadin by deamidationHitomi Kumagai
Department of Agricultural and Biological Chemistry, Nihon University, Fujisawa shi, Japan
Biosci Biotechnol Biochem 71:977-85. 2007..Deamidation of gliadin reduced its reactivity toward the sera of patients with wheat allergy. Rats administered with deamidated gliadin showed suppressed elevation of the gliadin-specific IgE level...
- Investigations into inhibitor type and mode, simulated gastrointestinal digestion, and cell transport of the angiotensin I-converting enzyme-inhibitory peptides in Pacific hake (Merluccius productus) fillet hydrolysateCrystal D Cinq-Mars
Food, Nutrition, and Health Program, Faculty of Land and Food Systems, 2205 East Mall, The University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4
J Agric Food Chem 56:410-9. 2008..Pacific hake fillet hydrolysates are a potentially bioavailable source of ACE-inhibitory peptides awaiting further in vivo study...
- Production of rice protein by alkaline extraction improves its digestibilityTakehisa Kumagai
Kameda Seika Co, Ltd, Niigata 950 0192, Japan
J Nutr Sci Vitaminol (Tokyo) 52:467-72. 2006..In an in vitro digestion study with pepsin and pancreatin, both the SDS-PAGE analysis of protein pattern and the crude protein content of undigested residue clearly ..
- An assessment of the bioavailability of persistent organic pollutants from contaminated soilW C Scott
School of Applied Sciences, Northumbria University, Ellison Building, Newcastle upon Tyne, UKNE1 8ST
J Environ Monit 7:710-5. 2005..4 to 4.0% while following intestinal extraction ranged from 6.6 to 12.7%. It has been found that the majority of POPs present i.e. >75%, would be excreted if consumed and not be absorbed in the gastrointestinal tract of humans...
- Stability of anthocyanins from commercial black currant juice under simulated gastrointestinal digestionAlija Uzunović
Institute for Quality Control of Medicines, Titova 9, 71 000 Sarajevo, Bosnia and Herzegovina
Bosn J Basic Med Sci 8:254-8. 2008..during in vitro digestion in gastric and intestinal fluid regarding whether appropriate enzyme (pepsin or pancreatin) was added or not...
- Structural and functional properties of amylose complexes with genisteinRevital Cohen
Faculty of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa, Israel
J Agric Food Chem 56:4212-8. 2008..showed high retention of genistein in simulated stomach conditions and released genistein upon digestion in pancreatin solution. It is therefore suggested that the complexes can be used as carriers for the slow release of genistein.
- Zein microspheres as drug/antigen carriers: a study of their degradation and erosion, in the presence and absence of enzymesPepi Hurtado-López
Department of Pharmaceutics, School of Pharmacy, University of London, London, UK
J Microencapsul 23:303-14. 2006..in the absence of enzymes (which explains the slow release of entrapped solute), but were degraded by pepsin and pancreatin enzymes in simulated gastric and intestinal fluids, respectively (which indicates that release is likely to be ..
- Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in vitro digestionAndrew P Neilson
Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA
J Agric Food Chem 55:8941-9. 2007..These data provide a logical basis for initial screening to detect catechin digestive products in vivo...
- Probiotic preparation has the capacity to hydrolyze proteins responsible for wheat allergyMaria de Angelis
Department of Plant Protection and Applied Microbiology, University of Bari, 70126 Bari, Italy
J Food Prot 70:135-44. 2007..allergic patients, several IgE-binding proteins persisted after treatment of baker's yeast bread by pepsin and pancreatin. The signal of all these IgE-binding proteins disappeared after further treatment by VSL#3...
- Validation and application of a stability-indicating HPLC method for the in vitro determination of gastric and intestinal stability of venlafaxineEbenezer B Asafu-Adjaye
Division of Product Quality Research, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, United States
J Pharm Biomed Anal 43:1854-9. 2007..This stability experiment in simulated gastric and intestinal fluids suggests that drug loss in the gastrointestinal tract takes place by membrane permeation rather than a degradation process...
- [Does enzyme substitution therapy reduce pain in chronic pancreatitis?]Marie Louise Hvass Hansen
Amtssygehuset i Gentofte, Medicinsk afdeling F
Ugeskr Laeger 167:1841-4. 2005
- Pancreatic enzyme extract improves survival in murine pancreatic cancerMurat Saruc
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Pancreas 28:401-12. 2004..Based on a recent report on the effectiveness of pancreatic enzyme therapy, we examined the effect of porcine pancreatic enzyme extracts (PPE) on human PC xenografts in nude mice...
- A dynamic in vitro lipolysis model. II: Evaluation of the modelN H Zangenberg
Department of Pharmaceutics, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark
Eur J Pharm Sci 14:237-44. 2001..This difference between danazol and probucol was attributed to the effect of different lipophilicity...
- Weaning and feed intake alter pancreatic enzyme activities and corresponding mRNA levels in 7-d-old pigletsJulia Marion
Unité Mixte de Recherches sur le Veau et le Porc, Institut National de la Recherche Agronomique, St Gilles, France
J Nutr 133:362-8. 2003..Therefore, the pancreas can adjust to weaning and dry food intake as early as wk 2 of life...
- Novel O-palmitoylscleroglucan-coated liposomes as drug carriers: development, characterization and interaction with leuprolideM Carafa
Dept Scienze del Farmaco, University G D Annunzio, Via dei Vestini, 66100 Chieti, Italy
Int J Pharm 325:155-62. 2006..bilayer fluidity, stability in serum, in simulated gastric and intestinal fluids and against sodium cholate and pancreatin. Leuprolide, a synthetic superpotent agonist of luteinizing hormone releasing hormone (LHRH) receptor, was ..
- Solubilisation of poorly water-soluble drugs during in vitro lipolysis of medium- and long-chain triacylglycerolsJanne Ørskov Christensen
Department of Pharmaceutics, The Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK 2100 Copenhagen, Denmark
Eur J Pharm Sci 23:287-96. 2004..Differences in the physicochemical properties of the drugs resulted in differences in their distribution between the phases arising during lipolysis...
- Improving digestibility of soy flour by reducing disulfide bonds with thioredoxinRichard J Faris
Department of Animal Science, Iowa State University, 1221 Kildee Hall, Ames, Iowa 50011, USA
J Agric Food Chem 56:7146-50. 2008..system (NTS) effectively reduced disulfide bonds in soy flour and increased protein digestibility by trypsin and pancreatin as measured by the pH stat method...
- [Effect of endogenous enzymes on composition of oligonucleotides isolated from gonads of hydrobionts]Iu M Pozdniakova
Pacific Research Fisheries Center TINRO Center, Vladivostok, 690950, Russia
Prikl Biokhim Mikrobiol 39:524-9. 2003..It was demonstrated that high activities of endogenous enzymes (nucleases and proteases) in milts of hydrobionts correlated with the degree of hydrolysis of their nucleic acids...
- Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tractW P Charteris
SET Consultants Ltd, Douglas, Cork, Ireland
J Appl Microbiol 84:759-68. 1998..3% w/v) and sodium chloride (0.5% w/v), and a simulated small intestinal juice (pH 8.0), containing pancreatin USP (1 g l-1) and sodium chloride (5 g l-1), and monitoring changes in total viable count periodically...
- Efficacy and tolerability of a new formulation of pancrelipase delayed-release capsules in children aged 7 to 11 years with exocrine pancreatic insufficiency and cystic fibrosis: a multicenter, randomized, double-blind, placebo-controlled, two-period crosGavin R Graff
Penn State Milton S Hershey Medical Center, Penn State Medical School, Department of Pediatrics, Hershey, Pennsylvania 17033 0850, USA
Clin Ther 32:89-103. 2010..The US Food and Drug Administration regulations now require all PERT products to undergo clinical efficacy and safety studies before they can be considered for marketing approval...
- The in vitro hydrolysis of phytosterol conjugates in food matrices by mammalian digestive enzymesRobert A Moreau
Crop Conversion Science and Engineering Research Unit, ERRC, ARS, USDA, Wyndmoor, Pennsylvania 19038, USA
Lipids 39:769-76. 2004..of hydrolysis of four common phytosterol conjugates by mammalian digestive enzymes (cholesterol esterase and pancreatin, a mixture of pancreatic enzymes) and for comparison purposes, by KOH...
- T cells expressing the gamma delta T cell receptor induce apoptosis in cardiac myocytesS A Huber
Department of Pathology, University of Vermont, Colchester 05446, USA
Cardiovasc Res 45:579-87. 2000..The objective of the present studies is to demonstrate that gamma delta T cells directly induce greater Fas-dependent apoptosis of cultured myocytes than T cells expressing the alpha beta TcR...
- Comparative studies on the in vitro properties of phytases from various microbial originsF A Igbasan
Freie Universitat Berlin, Institut für Tierernährung, Fachbereich Veterinarmedizin, Brümmer Strasse 34, D 14195 Berlin, Germany
Arch Tierernahr 53:353-73. 2000..coli phytase was insensitive against pepsin and pancreatin. Incubation of the enzymes in digesta supernatants from various segments of the digestive tract of hens revealed ..
- Biodegradable microspheres based on gelatin-porcine mucin admixtures: in vitro and in vivo delivery studiesKenneth Chibuzor Ofokansi
Drug Delivery Research Unit, Department of Pharmaceutics, University of Nigeria, Nsukka 410001, Enugu State, Nigeria
Biol Pharm Bull 32:1754-9. 2009..were evaluated for the in vitro delivery of cefaclor in both simulated intestinal fluid (SIF) without pancreatin (pH 7.4) and simulated gastric fluid (SGF) without pepsin (pH 1.2)...
- Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor modelPhilipp Ritter
Agroscope Liebefeld Posieux Research Station ALP, Schwarzenburgstrasse 161, CH 3003 Bern Liebefeld, Switzerland
BMC Microbiol 9:87. 2009..The advantage of this automated one reactor system was the ability to test the influence of acid, bile salts and pancreatin.Lactobacillus gasseri K7 is a strain isolated from infant faeces with properties making the strain interesting ..
- Starch-based coatings for colon-specific delivery. Part II: physicochemical properties and in vitro drug release from high amylose maize starch filmsC Freire
Institute of Pharmacy, Chemistry and Biomedical Sciences, University of Sunderland, Sunderland, UK
Eur J Pharm Biopharm 72:587-94. 2009..2. Selected formulations were further assessed in simulated gastric and intestinal fluids containing pepsin and pancreatin, respectively. The free films prepared were smooth and homogeneous in their appearance...
- Inhibition of transforming growth factor beta (TGFbeta1) expression and extracellular matrix secretion in human peritoneal mesothelial cells by pcDU6 vector-mediated TGFbeta1 shRNA and by pcDNA3.1(-)-mediated antisense TGFbeta1 RNAFuyou Liu
Division of Nephrology, Nephrology Institute of Central South University, The Second Xiangya Hospital, Central South University, Changsha, China
Adv Perit Dial 21:41-52. 2005..human TGFbeta1 antisense mammalian expression vector, and we isolated HPMCs from human greater omentum by pancreatin disaggregation to establish a stable cell-culture model...
- [Repair of facial nerve with bioactive artificial nerve conduit: experiment with rats]Huai Dong Du
Ear Nose Throat Department, Eye and Ear Nose Throat Hospital, Fudan University, Shanghai 200031, China
Zhonghua Yi Xue Za Zhi 87:3302-6. 2007..To evaluate the effect of bioactive artificial nerve conduits in the repair of facial nerve defects...
- A reliable method to determine methylmercury and ethylmercury simultaneously in foods by gas chromatography with inductively coupled plasma mass spectrometry after enzymatic and acid digestionStephen Wai Cheung Chung
Food Research Laboratory, Centre for Food Safety, Food and Environmental Hygiene Department, 382 Nam Cheong Street, Hong Kong, China
J Chromatogr A 1218:1260-5. 2011..Samples were digested with pancreatin and then hydrochloric acid...
- Probiotic properties of yeasts isolated from chicken feces and kefirsKatarzyna Rajkowska
Technical University of Łódź, Institute of Fermentation Technology and Microbiology, Łódź, Poland
Pol J Microbiol 59:257-63. 2010..3-93.7% after 4 hours of incubation in media with addition of 3 g/l pepsin and 1 g/l pancreatin. Kefir isolates were more sensitive to these conditions and a further 10% reduction of cell number in relation ..
- Pancreatic enzyme replacement therapy in post-pancreatectomy patientsM Braga
Department of Surgery, Scientific Institute H S Raffaele, Milano, Italy
Int J Pancreatol 5:37-44. 1989..Upon discharge from the hospital, patients received pancreatin powder as a dietary enzyme supplement (18,000 lipase U/meal)...
- Prediction of adsorption from multicomponent solutions by activated carbon using single-solute parametersD E Wurster
College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA
AAPS PharmSciTech 1:E25. 2000..of 3 barbiturates--phenobarbital, mephobarbital, and primidone--from simulated intestinal fluid (SIF), without pancreatin, by activated carbon was studied using the rotating bottle method...
- Ileal digestibility of amino acids, phosphorus, phytate and energy in pigs fed sorghum-based diets supplemented with phytase and Pancreatin®M Cervantes
Instituto de Ciencias Agricolas, Universidad Autonoma de Baja California, Mexicali, Mexico
J Anim Physiol Anim Nutr (Berl) 95:179-86. 2011..Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin(®) ) to growing pigs fed sorghum-soybean meal diets supplemented with phytase on the AID of AA, energy, and ..
- Arsenic bioaccessibility and speciation in clams and seaweed from a contaminated marine environmentI Koch
Environmental Sciences Group, Royal Military College of Canada, PO Box 17000 Station Forces, Kingston, ON, Canada K7K 7B4
Mar Pollut Bull 54:586-94. 2007..5 and glycine for 1h followed by pancreatin, bile extract and pH adjustment to 7 for an additional 4h) and speciation of arsenic in extracts (HPLC-HG-AAS to ..
- Metabolism evaluation of biomimetic prodrugs by in vitro models and mass spectrometryMuriel Lalanne
Universite Paris Sud, CNRS, UMR 8612, IFR 141, 5, rue J B Clement, F 92296 Chatenay Malabry, France
Int J Pharm 379:235-43. 2009..First, liposomal formulation of each prodrug was incubated with a lipolysis model based on pancreatin and analysed using liquid chromatography combined with tandem mass spectrometry (LC-MS/MS)...
- Cross-linking of hard gelatin carbamazepine capsules: effect of dissolution conditions on in vitro drug releaseHervé Marchais
Laboratoire de Pharmacie Galenique et Biopharmacie, ADEN UPRES EA 3234, UFR de Médecine Pharmacie, 22 Boulevard Gambetta, 76183 Rouen, France
Eur J Pharm Sci 19:129-32. 2003..SLS) was added in the dissolution medium at a concentration of 2% m/v both in SGF and SIF with pepsin and pancreatin, respectively. The percentage of CBZ dissolved was reduced by increasing the degree of gelatin cross-linking...
- Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysateLijuan Zhu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
J Agric Food Chem 56:2714-21. 2008..5-2 h of pancreatin, 37 degrees C) in vitro digestion...
- [Pancreatic extract therapy in exocrine pancreatic insufficiency]B Santini
Istituto di Discipline Pediatriche, Universita degli Studi di Torino, Cattedra di Pediatria
Minerva Gastroenterol Dietol 39:133-7. 1993..The authors consider all the different enzyme preparations from pancreatin powder to the 1st...
- Enzymatic recovery and purification of polyhydroxybutyrate produced by Ralstonia eutrophaFernanda M Kapritchkoff
Agrupamento de Biotecnologia DQ, Instituto de Pesquisas Tecnológicas do Estado de São Paulo IPT, Cidade Universitaria, Av Prof Almeida Prado 532, CEP 05508 901 São Paulo, SP, Brazil
J Biotechnol 122:453-62. 2006..Aiming at improving the process efficiency and reducing the enzyme cost, experiments were carried out with pancreatin, leading to 90.0% polymer purity and an enzyme cost three times lower than the one obtained with bromelain...
- Postprandial carbohydrate metabolism in healthy subjects and those with type 2 diabetes fed starches with slow and rapid hydrolysis rates determined in vitroChris J Seal
Human Nutrition Research Centre, School of Agriculture, Food and Rural Development, Faculty of Science, Agriculture and Engineering, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
Br J Nutr 90:853-64. 2003..the present study was to investigate the effects of starches with differing rates of hydrolysis on exposure to pancreatin in vitro on postprandial carbohydrate metabolism in healthy subjects and in subjects with type 2 diabetes...
- Bowman-Birk inhibitor and genistein among soy compounds that synergistically inhibit nitric oxide and prostaglandin E2 pathways in lipopolysaccharide-induced macrophagesVermont P Dia
Department of Food Science and Human Nutrition, University of Illinois at Urbana Champaign, 228 ERML, MC 051, 1201 West Gregory Drive, Urbana, Illinois 61801, USA
J Agric Food Chem 56:11707-17. 2008..A), saponin B group glycosides (saponin B), sapogenol B, Bowman-Birk inhibitor (BBI), lunasin, and pepsin-pancreatin glycinin hydrolysates were tested by measuring their ability to inhibit cyclooxygenase-2/prostaglandin E(2) (PGE(..
- Refining in vitro digestibility assays: fractionation of digestible and indigestible peptidesY Qiao
Department of Animal Science, North Carolina State University, Raleigh 27695, USA
J Anim Sci 82:1669-77. 2004..A method that exhaustively digests proteins using pepsin and pancreatin was developed, and the first objective of this research was to confirm that exhaustive digestion was indeed ..
- Neutralization assay for human group C rotaviruses using a reverse passive hemagglutination test for endpoint determinationR Fujii
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Okayama 701 0212, Okayama 700 8558, Japan
J Clin Microbiol 38:50-4. 2000..Maintenance medium containing 100 microgram of pancreatin per ml was placed in each well...
- Zymographic assay of plant diamine oxidase on entrapped peroxidase polyacrylamide gel electrophoresis. A study of stability to proteolysisCarmen Calinescu
Department of Chemistry and Centre BioMed, Universite du Quebec a Montreal, CP 8888, Succ A, Montreal, QC, H3C 3P8, Canada
Anal Bioanal Chem 396:1281-90. 2010..the DAO content from the vegetal extract was kept almost constant in the simulated intestinal fluid (containing pancreatin or not), with a slight diminution in the presence of pancreatic proteases...
- Food-protein enzymatic hydrolysates possess both antimicrobial and immunostimulatory activities: a "cause and effect" theory of bifunctionalityGediminas A Biziulevicius
Immunomodulators Research Sector, Institute of Immunology, Vilnius University, 29 Moletu plentas, LT 08409 Vilnius, Lithuania
FEMS Immunol Med Microbiol 46:131-8. 2006..and serum albumin) hydrolyzed with four gastrointestinal proteinases (trypsin, alpha-chymotrypsin, pepsin and pancreatin)] were examined...
- Stabilization of a supplemental digestive enzyme by post-translational engineering using chemically-activated polyethylene glycolKevin M Turner
Industrial Biochemistry Program, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
Biotechnol Lett 33:617-21. 2011..Conjugation also provided significant protection against the proteolytic component of pancreatin. Overall, the PEGylated enzyme retained over twice the levels of residual activity recorded for non-PEGylated ..
- In vitro digestibility techniques to predict apparent total tract energy digestibility of wheat in grower pigsP R Regmi
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada
J Anim Sci 87:3620-9. 2009..5 mL) for 6 h, pancreatin (150 mg/54.5 mL) for 18 h, and cellulase (75 mg/55.5 mL) for 24 h. In IVD-VIS, 0...
- Changes in the antioxidative property of herring (Clupea harengus) press juice during a simulated gastrointestinal digestionThippeswamy Sannaveerappa
Department of Chemical and Biological Engineering, Food Science, Chalmers University of Technology, Goteborg, Sweden
J Agric Food Chem 55:10977-85. 2007..and light muscle (LMPJ) was mixed with pepsin solution followed by stepwise pH adjustments and additions of pancreatin and bile solutions. Digestive enzymes were removed from samples by ultrafiltration (10 kDa)...
- [The progesterone-induced expression of cyclin G1 and its effect on the proliferation of mouse uterine epithelial cells]Yan Yan Ma
Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China
Sheng Li Xue Bao 60:541-6. 2008..Then the uteri were digested by dispase and pancreatin respectively...
- Bifidobacteria inhibit the inflammatory response induced by gliadins in intestinal epithelial cells via modifications of toxic peptide generation during digestionJ M Laparra
Instituto de Agroquimica y Tecnologia de Alimentos CSIC, Burjassot Valencia, Spain
J Cell Biochem 109:801-7. 2010..alpha, beta, gamma, [symbol: see text] ) was subjected to in vitro gastrointestinal digestion (pepsin at pH 3, pancreatin-bile at pH 6), inoculated or not with cell suspensions (10(8) colony forming units/ml) of either B...
- New modalities in the treatment of exocrine pancreatic insufficiency in cystic fibrosisH G Heijerman
Leyenburg Hospital, Department of Pulmonology, The Hague, Netherlands
Neth J Med 41:105-9. 1992..potency and duration of action compared to H2-antagonists, improves the efficacy of enteric-coated capsules of pancreatin dramatically...
- Iron uptake by Caco-2 cells following in vitro digestion: effects of heat treatments of pork meat and pH of the digestsAnne D Sørensen
Department of Human Nutrition Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, Frederiksberg C, Denmark
J Trace Elem Med Biol 24:230-5. 2010The present in vitro studies report on iron uptake by Caco-2 cells from pepsin and pepsin+pancreatin-digested pork meat proteins at pH values between 4.6 and 7 mimicking conditions in the duodenum and the proximal jejunum, respectively...
- Ruminal degradation and intestinal digestibility of dried or wet distillers grains with increasing concentrations of condensed distillers solublesZ J Cao
Dairy Science Department, South Dakota State University, Brookings, SD 57007, USA
J Anim Sci 87:3013-9. 2009..Intestinal CP digestibility was measured on residue from 12-h ruminal incubation feeds by the pepsin-pancreatin method. The soluble fraction of DM and CP was greater (P < 0...
- Voluntary intake, chemical composition and in vitro digestibility of fresh forages fed to guinea pigs in periurban rearing systems of Kinshasa (Democratic Republic of Congo)J Bindelle
Department of Animal Husbandry, Gembloux Agricultural University, 2 Passage des Deportes, 5030 Gembloux, Belgium
Trop Anim Health Prod 39:419-26. 2007..combination for GP diet, DVI was compared to their nutritional value measured in vitro using (1) a pepsin-pancreatin hydrolysis, (2) an gas fermentation test on the hydrolysed residues with an inoculum prepared from GP faeces, ..
- A method for the dispersal and characterization of leukocytes from the human female reproductive tractH D White
Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756 0001, USA
Am J Reprod Immunol 44:96-103. 2000..Here, we describe the isolation of RT cells in high yields and with high viability from the Fallopian tube, uterine endometrium, endocervix, ectocervix and vagina...
- Ionotropic cross-linked chitosan microspheres for controlled release of ampicillinAnil K Anal
Riddet Centre, Massey University, Private Bag 11222, Palmerston North, New Zealand
Int J Pharm 312:166-73. 2006..2) and simulated intestinal fluid (SIF, pH 7.5). The inclusion of the enzymes pepsin and pancreatin did not affect the stability of the microspheres...
- Stability of liposomes containing bio-enhancers and tetraether lipids in simulated gastro-intestinal fluidsJohannes Parmentier
Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Technology and Biopharmacy, University of Heidelberg, Im Neuenheimer Feld 366, D 69120 Heidelberg, Germany
Int J Pharm 405:210-7. 2011..bio-enhancers cholylsarcosine, octadecanethiol and TPGS 1000 in Tris buffer pH 2, sodium taurocholate 10mM and pancreatin was compared...
- Met-Arg-Trp derived from Rubisco lowers blood pressure via prostaglandin D(2)-dependent vasorelaxation in spontaneously hypertensive ratsHui Zhao
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Gokasho Uji, Kyoto, Japan
Peptides 29:345-9. 2008Met-Arg-Trp (MRW) has been isolated as an inhibitor for angiotensin I-converting enzyme (ACE) from a pepsin-pancreatin digest of spinach ribulose bisphosphate carboxylase/oxygenase (Rubisco) (IC(50)=0.6 microM)...
- Identification and quantitation of extractables from cellulose acetate butyrate (CAB) and estimation of their in vivo exposure levelsDecheng Ma
Merck Research Laboratories, Pharmaceutical Analysis and Control, P O Box 4, WP14 2E, West Point, PA 19486, USA
J Pharm Biomed Anal 35:779-88. 2004..After 24 h exposure in USP simulated fluids, the maximum amount of EHA extracted corresponds to < 0.3 microg of EHA per tablet. Pepsin and pancreatin in USP simulated fluids had no effect on EHA extraction and quantitation.
- Antioxidant and bile acid binding activity of buckwheat protein in vitro digestsYuanyuan Ma
Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky 40546, USA
J Agric Food Chem 57:4372-80. 2009..potential of buckwheat protein (BWP) during a two-stage in vitro digestion (1 h of pepsin followed by 2 h of pancreatin)...
- Enzyme-responsive intracellular controlled release using nanometric silica mesoporous supports capped with "saccharides"Andrea Bernardos
Instituto de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Centro Mixto Universidad Politécnica de Valencia Universidad de Valencia, Spain
ACS Nano 4:6353-68. 2010..Delivery studies in pure water in the presence of pancreatin or β-d-galactosidase were carried out for S1-S3 and S4, respectively...
- A gene-shuffled glyphosate acetyltransferase protein from Bacillus licheniformis (GAT4601) shows no evidence of allergenicity or toxicityBryan Delaney
Pioneer Hi Bred International, Inc, Johnston, Iowa 50131, USA
Toxicol Sci 102:425-32. 2008..degraded in simulated gastric fluid containing pepsin (< 30 s) and in simulated intestinal fluid containing pancreatin (< 2 min) and completely inactivated at temperatures above 56 degrees C...
- Effects of phytases and dehulling treatments on in vitro iron and zinc bioavailability in faba bean (Vicia faba L.) flour and legume fractionsYuwei Luo
College of Horticulture, Jinling Institute of Technology, 210038 Nanjing, P R China
J Food Sci 75:C191-8. 2010..Faba bean samples were sequentially digested with enzymes, including amylase, pepsin, pancreatin, and bile, under certain conditions following the enzymatic degradation procedure...
- Dietary glycosaminoglycans interfere in bacterial adhesion and gliadin-induced pro-inflammatory response in intestinal epithelial (Caco-2) cellsJ M Laparra
Institute of Agrochemistry and Food Technology, Spanish National Research Council CSIC, PO Box 73, 46100 Burjassot, Valencia, Spain
Int J Biol Macromol 47:458-64. 2010..Gliadins were subjected to in vitro digestion (pepsin/pancreatin-bile), with/out GAGs, and the presence or not of cell suspensions of B. longum (10(8) CFU/ml). B. longum, E...
- Bioavailability of insect growth regulators in citrus and stone fruitsP Paya
Departamento Quimica Agricola, Geologia y Edafologia, Facultad de Quimica, Universidad de Murcia, Campus de Espinardo, S N, 30100, Espinardo, Murcia, Spain
Commun Agric Appl Biol Sci 72:151-9. 2007..The intestinal digestion was simulated with porcine pancreatin at pH 7, for 2 h in a shaking water bath at 37 degrees C...
- Nutritive value of unconventional fibrous ingredients fed to Guinea pigs in the Democratic Republic of CongoJ Bindelle
Gembloux Agricultural University, Animal Science Unit, 2 Passage des Deportes, 5030 Gembloux, Belgium
Trop Anim Health Prod 41:1731-40. 2009..The forages and the diets were digested in vitro using pepsin and pancreatin hydrolysis and gas fermentation test to simulate stomach, small intestine and large intestine, respectively...
- Breakthroughs to advance the in vitro propagation of human norovirusesQiuhong Wang; Fiscal Year: 2010..or conditions mimicking the intestinal microenvironment include intestinal content filtrates, bile acids and pancreatin that enhance propagation of fastidious enteric viruses, including enteric caliciviruses...
- 14th Internatinal Symposium on Inherited DiseasesDavid Whitcomb; Fiscal Year: 2004..The format, venue, and content are designed to maximize participation, education, translation and implementation of the key elements of the many new findings in the genetics of the pancreas. ..
- Studying the interaction of Flor-Essence Tonic and PHIPKristen Kulp; Fiscal Year: 2004..It is important to determine how currently used CAMs are interacting with dietary carcinogens to influence health. Science based information is needed so that people can make informed lifestyle choices. ..
- Microvascular Injury and Pancreatic Enzyme ProductsGEERT SCHMID SCHOENBEIN; Fiscal Year: 2005..Understanding the trigger mechanisms for inflammation in shock will lead to new treatment modalities in man. ..
- A Novel Iron-Binding Component of Breast MilkRobert Serfass; Fiscal Year: 2004..The proposed studies are essential prerequisites to future studies concerning the purported effects of the low molecular weight substance(s) in breast milk on iron absorption in animal models and in infants. ..
- MOLECULAR GENETICS OF HEREDITARY PANCREATITISDavid Whitcomb; Fiscal Year: 2008..The HP and familial pancreatitis studies are especially important because of the statistical power of family studies and the ability to detect modifier genes in a uniform background of susceptibility factors. ..
- 2007 Molecular Mechansims in Lymphatic Function & DiseaseGEERT SCHMID SCHOENBEIN; Fiscal Year: 2008..End of Abstract) [unreadable] [unreadable] [unreadable]..
- NAPS2 Continuation - Genome-Wide Association Study of PancreatitisDavid C Whitcomb; Fiscal Year: 2010..Knowledge of the genetic basis of pancreatitis will lead to new treatments, and help remove the stigma that chronic pancreatitis is only a disease of drunkards. ..
- 2006 Molecular Mechanisms in Lymphatic Function & Disease Gordon ConferenceGEERT SCHMID SCHOENBEIN; Fiscal Year: 2006..The conference plays an essential role in the advancement of lymphology and development of a new understanding of lymphatic diseases and treatments. [unreadable] [unreadable] [unreadable] [unreadable]..