peptide hydrolases

Summary

Summary: Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.

Top Publications

  1. ncbi Improved prediction of signal peptides: SignalP 3.0
    Jannick Dyrløv Bendtsen
    Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Mol Biol 340:783-95. 2004
  2. ncbi Targeting proteases: successes, failures and future prospects
    Boris Turk
    Department of Biochemistry and Molecular Biology, J Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Nat Rev Drug Discov 5:785-99. 2006
  3. ncbi Emerging roles of proteases in tumour suppression
    Carlos Lopez-Otin
    Carlos López Otín is at the Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Nat Rev Cancer 7:800-8. 2007
  4. ncbi Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome
    Rati Verma
    Department of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:611-5. 2002
  5. ncbi The COP9 signalosome: more than a protease
    Ning Wei
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Trends Biochem Sci 33:592-600. 2008
  6. ncbi Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products
    Oded Kleifeld
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Nat Biotechnol 28:281-8. 2010
  7. ncbi The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage
    Regina Groisman
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 113:357-67. 2003
  8. ncbi A cryptic protease couples deubiquitination and degradation by the proteasome
    Tingting Yao
    Department of Biochemistry, University of Iowa, 51 Newton Road, Iowa City, Iowa 52242, USA
    Nature 419:403-7. 2002
  9. pmc Malarial proteases and host cell egress: an 'emerging' cascade
    Michael J Blackman
    Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Cell Microbiol 10:1925-34. 2008
  10. pmc Variably protease-sensitive prionopathy: a new sporadic disease of the prion protein
    Wen Quan Zou
    Department of Pathology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Ann Neurol 68:162-72. 2010

Research Grants

  1. INTESTINAL PEPTIDE HYDROLASES AND PEPTIDE TRANSPORT
    Young Kim; Fiscal Year: 2000
  2. The Penn Center For Molecular Discovery
    Scott Diamond; Fiscal Year: 2008
  3. Novel Roles for ADAM15 in Acute Lung Injury
    Caroline A Owen; Fiscal Year: 2010
  4. Positional cloning of a gene for mental retardation
    Joseph Higgins; Fiscal Year: 2004
  5. Multiplexed HTS of Serine and Cysteine Proteases (RMI)
    Scott Diamond; Fiscal Year: 2005
  6. Blood Systems Biology
    Scott Diamond; Fiscal Year: 2008
  7. Novel Roles for MMP-8 in Acute Lung Injury
    Caroline Owen; Fiscal Year: 2008
  8. Proteomics/Genomics of Opiate Analgesia and Addiction
    Vivian Hook; Fiscal Year: 2004
  9. Reaction and Transport Dynamics in Human Blood
    Scott Diamond; Fiscal Year: 2009
  10. Role of cockroach proteases in airway inflammation
    Kristen Page; Fiscal Year: 2009

Detail Information

Publications311 found, 100 shown here

  1. ncbi Improved prediction of signal peptides: SignalP 3.0
    Jannick Dyrløv Bendtsen
    Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    J Mol Biol 340:783-95. 2004
    ..The new method has been benchmarked against other available methods. Predictions can be made at the publicly available web server..
  2. ncbi Targeting proteases: successes, failures and future prospects
    Boris Turk
    Department of Biochemistry and Molecular Biology, J Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Nat Rev Drug Discov 5:785-99. 2006
    ..The status of human protease research and prospects for future protease-targeted drugs are reviewed here, with reference to some key examples where protease drugs have succeeded or failed...
  3. ncbi Emerging roles of proteases in tumour suppression
    Carlos Lopez-Otin
    Carlos López Otín is at the Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Nat Rev Cancer 7:800-8. 2007
    ..These effects are associated with more than 30 different enzymes that belong to three distinct protease classes. What are the clinical implications of these findings?..
  4. ncbi Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome
    Rati Verma
    Department of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:611-5. 2002
    ..Our findings reveal an unexpected coupling between substrate deubiquitination and degradation and suggest a unifying rationale for the presence of the lid in eukaryotic proteasomes...
  5. ncbi The COP9 signalosome: more than a protease
    Ning Wei
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Trends Biochem Sci 33:592-600. 2008
    ..The independent functions of CSN5 (also known as JAB1) add to the complexity of the CSN machinery. Here, we provide an updated view of the structure, functions and regulation of this protein complex...
  6. ncbi Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products
    Oded Kleifeld
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Nat Biotechnol 28:281-8. 2010
    ....
  7. ncbi The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage
    Regina Groisman
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 113:357-67. 2003
    ..Knockdown of CSN with RNA interference leads to defects in NER. These results suggest that the distinct UV response of the DDB2 and CSA complexes is involved in diverse mechanisms of NER...
  8. ncbi A cryptic protease couples deubiquitination and degradation by the proteasome
    Tingting Yao
    Department of Biochemistry, University of Iowa, 51 Newton Road, Iowa City, Iowa 52242, USA
    Nature 419:403-7. 2002
    ..The inability to remove ubiquitin can be rate-limiting for degradation in vitro and is lethal to yeast. Unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases, POH1 appears to be a Zn(2+)-dependent protease...
  9. pmc Malarial proteases and host cell egress: an 'emerging' cascade
    Michael J Blackman
    Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Cell Microbiol 10:1925-34. 2008
    ..These findings are discussed in the context of the potential for development of new chemotherapeutics targeting this stage in the parasite's life cycle...
  10. pmc Variably protease-sensitive prionopathy: a new sporadic disease of the prion protein
    Wen Quan Zou
    Department of Pathology, National Prion Disease Pathology Surveillance Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Ann Neurol 68:162-72. 2010
    ..The 2 new PSPr forms affect individuals who are either homozygous for methionine (129MM) or heterozygous for methionine/valine (129MV)...
  11. pmc Jab1/CSN5 negatively regulates p27 and plays a role in the pathogenesis of nasopharyngeal carcinoma
    Yunbao Pan
    Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat Sen University, Guangzhou, Guangdong, PR China
    Cancer Res 72:1890-900. 2012
    ..Jab1 therefore represents a novel diagnostic marker and therapeutic target in patients with NPC...
  12. ncbi Mammalian COP9 signalosome
    Jun ya Kato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    Genes Cells 14:1209-25. 2009
    ..This review summarizes recent findings and discusses the physiological role and the cellular function of the mammalian CSN in terms of the regulation of cell proliferation...
  13. pmc A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing
    Gwendolyn M Wilmes
    Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, Genentech Hall, San Francisco, CA 94143 2200, USA
    Mol Cell 32:735-46. 2008
    ..Finally, we show that Csn12 plays a role in pre-mRNA splicing, which is independent of other signalosome components. Thus, Sem1 is involved in three separate and functionally distinct complexes...
  14. ncbi Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites
    Oliver Schilling
    The UBC Centre for Blood Research, Department of Oral Biological and Medical Sciences, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Nat Biotechnol 26:685-94. 2008
    ..Profiling of HIV protease 1, caspase 3, caspase 7, cathepsins K and G, elastase and thrombin showed that this approach is broadly applicable to all mechanistic classes of endoproteases...
  15. ncbi Neddylation and deneddylation regulate Cul1 and Cul3 protein accumulation
    June Tai Wu
    Institute of Molecular Biology, Academia Sinica, 115, Taipei, Taiwan
    Nat Cell Biol 7:1014-20. 2005
    ..Thus, CSN deneddylation recycles the unstable, neddylated cullins into stable, unneddylated ones, and promotes cullin-organized E3 activity in vivo...
  16. pmc An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation
    Silke Busch
    Institut fur Mikrobiologie und Genetik, Georg August Universitat, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:8089-94. 2007
    ..We propose that a correctly assembled CSN including a functional JAMM links protein turnover to fungal sexual development...
  17. ncbi A cut above the rest: the regulatory function of plant proteases
    Andreas Schaller
    Institute of Plant Physiology and Biotechnology 260, University of Hohenheim, 70593 Stuttgart, Germany
    Planta 220:183-97. 2004
    ..This article reviews recent findings for the major catalytic classes, i.e. the serine, cysteine, aspartic, and metalloproteases, emphasizing the regulatory function of representative enzymes...
  18. ncbi The cytoplasmic shuttling and subsequent degradation of p27Kip1 mediated by Jab1/CSN5 and the COP9 signalosome complex
    Kiichiro Tomoda
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    J Biol Chem 277:2302-10. 2002
    ..These results indicate that cytoplasmic shuttling regulated by Jab1/CSN5 and other CSN components may be a new pathway to control the intracellular abundance of the key cell cycle regulator...
  19. ncbi Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp. strain AB1 with high stability in organic solvents
    Bassem Jaouadi
    Laboratoire d Enzymes et de Metabolites des Procaryotes LEMP, Centre de biotechnologie de Sfax CBS, B P 1177, Route de Sidi Mansour Km 6, 3018 Sfax, Tunisia
    Bioresour Technol 101:8361-9. 2010
    ..These properties make KERAB a potential candidate for future applications in detergent formulations, dehairing during leather processing, and non-aqueous peptide biocatalysis...
  20. ncbi The COP9 signalosome
    Ning Wei
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Annu Rev Cell Dev Biol 19:261-86. 2003
    ..The involvement of CSN in a number of cellular and developmental processes has been attributed to its control over ubiquitin-proteasome-mediated protein degradation...
  21. pmc COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system
    D Bech-Otschir
    Division of Molecular Biology, Department of Surgery and Institute of Biochemistry, Medical Faculty Charité, Humboldt University, Monbijoustrasse 2, 10117 Berlin, Germany
    EMBO J 20:1630-9. 2001
    ..It induces the cyclin-dependent inhibitor p21. In HeLa and MCF-7 cells, inhibition of CSN kinase by curcumin or Deltap53(145-164) results in accumulation of endogenous p53...
  22. pmc Degradation of human antimicrobial peptide LL-37 by Staphylococcus aureus-derived proteinases
    Magdalena Sieprawska-Lupa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Antimicrob Agents Chemother 48:4673-9. 2004
    ..Taken together, these data suggest that aureolysin production by S. aureus contributes to the resistance of this pathogen to the innate immune system of humans mediated by LL-37...
  23. pmc Emerging principles in protease-based drug discovery
    Marcin Drag
    Program in Apoptosis and Cell Death Research, Burnham Institute for Medical Research, La Jolla, California 92037, USA
    Nat Rev Drug Discov 9:690-701. 2010
    ....
  24. pmc F-box-directed CRL complex assembly and regulation by the CSN and CAND1
    Michael W Schmidt
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Mol Cell 35:586-97. 2009
    ..These findings were integrated into a refined model of CRL control in which substrate availability toggles CRLs between independent CSN and CAND1 cycles...
  25. ncbi Regulatory subunit interactions of the 26S proteasome, a complex problem
    K Ferrell
    Dept of Biochemistry, University of Utah, 50 N Medical Drive, Salt Lake City, UT 84132, USA
    Trends Biochem Sci 25:83-8. 2000
    ..Inspection of interactions of regulatory subunits with non-subunit proteins reveals patterns that suggest these interactions play a role in 26S proteasome regulation and localization...
  26. ncbi The MEROPS batch BLAST: a tool to detect peptidases and their non-peptidase homologues in a genome
    Neil D Rawlings
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Biochimie 90:243-59. 2008
    ..A non-peptidase homologue can be distinguished by the absence or unacceptable replacement of any of these residues. An analysis of peptidase homologues in the genome of the bacterium Erythrobacter litoralis is presented as an example...
  27. pmc Corruption of innate immunity by bacterial proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 1:70-87. 2009
    ..It is vital that such mechanisms are elucidated to allow strategies to be developed to aid the innate immune system in controlling bacterial infections...
  28. ncbi Proteases in parasitic diseases
    James H McKerrow
    Department of Pathology, Sandler Center, University of California, San Francisco, California 94143, USA
    Annu Rev Pathol 1:497-536. 2006
    ....
  29. ncbi Symmetrical modularity of the COP9 signalosome complex suggests its multifunctionality
    Michal Sharon
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Structure 17:31-40. 2009
    ..This suggests that the propensity of the CSN complex to change and adapt its subunit composition might underlie its ability to perform multiple functions in vivo...
  30. ncbi Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1
    Gregory A Cope
    Department of Biology, California Institute of Technology Caltech, Pasadena, CA 91125, USA
    Science 298:608-11. 2002
    ..We propose that JAMM isopeptidases play important roles in a variety of physiological pathways...
  31. ncbi Sem1p is a novel subunit of the 26 S proteasome from Saccharomyces cerevisiae
    Takayuki Sone
    Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    J Biol Chem 279:28807-16. 2004
    ..The results suggest that Sem1, possibly hDSS1, is a novel subunit of the 26 S proteasome and plays a role in ubiquitin-dependent proteolysis...
  32. pmc Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease
    Terrence W James
    Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
    Proc Natl Acad Sci U S A 108:2222-7. 2011
    ....
  33. pmc Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity
    Elin Hadler-Olsen
    Institute of Medical Biology, Faculty of Medicine, University of Tromsø, 9037 Tromsø, Norway
    J Histochem Cytochem 58:29-39. 2010
    ..The yield, as analyzed by SDS-PAGE gelatin zymography and Western blotting, was influenced by the composition of the extraction buffer, but was generally lower than that obtained from unfixed tissue...
  34. pmc Expression and secretion of Aspergillus fumigatus proteases are regulated in response to different protein substrates
    Edward Farnell
    Respiratory Research Group, School of Translational Medicine, University of Manchester, Manchester M13 9PT, UK
    Fungal Biol 116:1003-12. 2012
    ..There is a requirement for the standardisation of A. fumigatus allergen extracts used both in clinical diagnosis of A. fumigatus allergy and in research studies...
  35. ncbi High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone
    Daniel Heintel
    Department of Internal Medicine I, Center for Oncology and Haematology, Wilhelminenspital, Vienna, Austria
    Br J Haematol 161:695-700. 2013
    ..CRBN expression is closely associated with IRF4, which is an important target of IMiD therapy...
  36. pmc Global identification of peptidase specificity by multiplex substrate profiling
    Anthony J O'Donoghue
    Department of Pharmaceutical Chemistry, University of California, San Francisco, USA
    Nat Methods 9:1095-100. 2012
    ..Additionally, the proteolytic secretions from schistosome parasitic flatworm larvae and a pancreatic cancer cell line were deconvoluted in a subtractive strategy using class-specific peptidase inhibitors...
  37. pmc Neutrophil granulocyte-dependent proteolysis enhances platelet adhesion to the arterial wall under high-shear flow
    N Wohner
    Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary
    J Thromb Haemost 8:1624-31. 2010
    ..Under high shear stress platelets adhere preferentially to the adventitia layer of the arterial vessel wall in a von Willebrand factor (VWF)-dependent manner...
  38. ncbi Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa
    Nathalie Beaufort
    Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Strasse 22, D 81675 Munich, Germany
    Biochem J 428:473-82. 2010
    ..Direct pro-uPA activation, as also reported for other bacterial proteases, might be a frequent phenomenon that contributes to bacterial virulence...
  39. pmc Molecular targeting of CSN5 in human hepatocellular carcinoma: a mechanism of therapeutic response
    Y H Lee
    Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, MD, USA
    Oncogene 30:4175-84. 2011
    ..Taken together, these results indicate that CSN5 has a pivotal role in HCC pathogenesis and maybe an attractive molecular target for systemic HCC therapy...
  40. pmc Sensing of 'danger signals' and pathogen-associated molecular patterns defines binary signaling pathways 'upstream' of Toll
    Laure El Chamy
    Institut de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Propre de Recherche 9022, Universite Louis Pasteur, Strasbourg Cedex, France
    Nat Immunol 9:1165-70. 2008
    ..Hence, Persephone defines a parallel proteolytic cascade activated by 'danger signals' such as abnormal proteolytic activities...
  41. ncbi The Arabidopsis COP9 signalosome is essential for G2 phase progression and genomic stability
    Esther M N Dohmann
    Tubingen University, Center for Plant Molecular Biology, Department of Developmental Genetics, Auf der Morgenstelle 3 5, 72076 Tubingen, Germany
    Development 135:2013-22. 2008
    ..In summary, our data suggest that Arabidopsis csn mutants undergo DNA damage, which might be the cause of the delay in G2 cell cycle progression...
  42. pmc MEROPS: the peptidase database
    Neil D Rawlings
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Nucleic Acids Res 38:D227-33. 2010
    ..This has allowed us to add an analysis tool to the relevant species pages to show significant gains and losses of peptidase genes relative to related species...
  43. ncbi High cereblon expression is associated with better survival in patients with newly diagnosed multiple myeloma treated with thalidomide maintenance
    Annemiek Broyl
    Department of Hematology, Erasmus MC, Rotterdam, The Netherlands
    Blood 121:624-7. 2013
    ..trialregister.nl) as NTR213; at the European Union Drug Regulating Authorities Clinical Trials (EudraCT) as 2004-000944-26; and at the International Standard Randomized Controlled Trial Number (ISRCTN) as 64455289...
  44. ncbi Proteomic identification of protease cleavage sites characterizes prime and non-prime specificity of cysteine cathepsins B, L, and S
    Martin L Biniossek
    Institute for Molecular Medicine and Cell Research, University of Freiburg, Germany
    J Proteome Res 10:5363-73. 2011
    ..0 and 7.5. The PICS profiles at both pH values display a high degree of similarity. Cathepsin S specificity is primarily guided by aliphatic residues in P2 with limited importance of prime-site residues...
  45. ncbi Phenotypic heterogeneity in inherited prion disease (P102L) is associated with differential propagation of protease-resistant wild-type and mutant prion protein
    Jonathan D F Wadsworth
    MRC Prion Unit and Department of Neurodegenerative Disease, Institute of Neurology, University College London, National Hospital for Neurology and Neurosurgery Queen Square, London, UK
    Brain 129:1557-69. 2006
    ..Such differential propagation of disease-related isoforms of wild-type PrP and PrP 102L provides a molecular mechanism for generation of the multiple clinicopathological phenotypes seen in inherited prion disease...
  46. pmc Acylpeptide hydrolase inhibition as targeted strategy to induce proteasomal down-regulation
    Gianna Palmieri
    Institute of Protein Biochemistry, National Research Council CNR IBP, Napoli, Italy
    PLoS ONE 6:e25888. 2011
    ..Our study supports a previously unrecognized role of APEH as a negative effector of proteasome activity by an unknown mechanism and opens new perspectives for the development of strategies aimed at modulation of cancer progression...
  47. pmc The Arabidopsis COP9 signalosome subunit 7 is a model PCI domain protein with subdomains involved in COP9 signalosome assembly
    Moshe Dessau
    Department of Biochemistry, Daniella Rich Institute for Structural Biology, Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
    Plant Cell 20:2815-34. 2008
    ..Together, the findings have implications for CSN assembly and function, highlighting necessary interactions between subunits...
  48. pmc Back signaling by the Nrg-1 intracellular domain
    Jianxin Bao
    Institute of Human Nutrition, 701 West 168th St, 5 503 New York, NY 10032, USA
    J Cell Biol 161:1133-41. 2003
    ..Thus, regulated proteolytic processing of Nrg-1 results in retrograde signaling that appears to mediate contact and activity-dependent survival of Nrg-1-expressing neurons...
  49. pmc The Legionella HtrA homologue DegQ is a self-compartmentizing protease that forms large 12-meric assemblies
    Robert Wrase
    Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Proc Natl Acad Sci U S A 108:10490-5. 2011
    ..Our study sheds light on a previously uncharacterized component of the prokaryotic stress-response system with implications for other members of the HtrA family...
  50. ncbi Protein degradation and protection against misfolded or damaged proteins
    Alfred L Goldberg
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 426:895-9. 2003
    ..A full understanding of the pathogenesis of the protein-folding diseases will require greater knowledge of how misfolded proteins are recognized and selectively degraded...
  51. pmc Gelatinase contributes to the pathogenesis of endocarditis caused by Enterococcus faecalis
    Lance R Thurlow
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Infect Immun 78:4936-43. 2010
    ..Taken together, these observations suggest that of the two enterococcal proteases, gelatinase is the principal mediator of pathogenesis in endocarditis...
  52. pmc Structure and catalysis of acylaminoacyl peptidase: closed and open subunits of a dimer oligopeptidase
    Veronika Harmat
    Laboratory of Structural Chemistry and Biology and HAS ELTE Protein Modeling Group, Institute of Chemistry, Eotvos Lorand University, Pázmány P sétány 1 A, H 1117 Budapest, Hungary
    J Biol Chem 286:1987-98. 2011
    ..The mutations affected the closed, active form of the enzyme, disrupting its catalytic triad. We concluded that the two forms are at equilibrium and the substrates bind by the conformational selection mechanism...
  53. ncbi Fungal proteases and their pathophysiological effects
    Iwona Yike
    Department of Environmental Health Sciences, School of Medicine, Case Western Reserve University, Location code 4940, Room WG19, 2109 Adelbert Rd, Cleveland, OH 44106, USA
    Mycopathologia 171:299-323. 2011
    ..The involvement of fungal proteases in diverse pathological mechanisms makes them potential targets of therapeutic intervention and candidates for biomarkers of disease and exposure...
  54. pmc Role of individual subunits of the Neurospora crassa CSN complex in regulation of deneddylation and stability of cullin proteins
    Jiyong Wang
    State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China
    PLoS Genet 6:e1001232. 2010
    ....
  55. pmc Mast cells
    Terez Shea-Donohue
    Department of Medicine and Mucosal Biology Research Center, University of Maryland School of Medicine, 20 Penn Street, Baltimore, MD 21201, USA
    Curr Gastroenterol Rep 12:349-57. 2010
    ..The purpose of this review article is to summarize and highlight the latest findings in mast cell biology associated with intestinal homeostasis and pathologies...
  56. pmc Origin and function of ubiquitin-like proteins
    Mark Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, PO Box 208114, New Haven, Connecticut 06520, USA
    Nature 458:422-9. 2009
    ....
  57. pmc Extracellular matrix proteases contribute to progression of pelvic organ prolapse in mice and humans
    Madhusudhan Budatha
    Department of Molecular Biology, University of Texas, Southwestern Medical Center, Dallas, Texas, USA
    J Clin Invest 121:2048-59. 2011
    ..These results suggest that POP is an acquired disorder of extracellular matrix and that therapies targeting matrix proteases may be successful for preventing or ameliorating POP in women...
  58. pmc Asparagine peptide lyases: a seventh catalytic type of proteolytic enzymes
    Neil David Rawlings
    Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    J Biol Chem 286:38321-8. 2011
    ..We have assembled ten families based on sequence similarity in which cleavage is thought to be catalyzed by an asparagine...
  59. pmc COP9 signalosome subunit 6 stabilizes COP1, which functions as an E3 ubiquitin ligase for 14-3-3σ
    H H Choi
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, USA
    Oncogene 30:4791-801. 2011
    ..These data suggest that the CSN6-COP1 axis is involved in 14-3-3σ degradation, and that deregulation of this axis will promote cell growth and tumorigenicity...
  60. pmc Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions
    James C Whisstock
    From the Department of Biochemistry and Molecular Biology and ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria 3800, Australia
    J Biol Chem 285:24307-12. 2010
    ..Finally, recent structural studies reveal how the serpin fold has been adapted for non-inhibitory functions such as hormone binding...
  61. ncbi Recent advances in targeting viral proteases for the discovery of novel antivirals
    Holger Steuber
    Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Curr Top Med Chem 10:323-45. 2010
    ....
  62. pmc Targeting Jab1/CSN5 in nasopharyngeal carcinoma
    Yunbao Pan
    Department of Systems Biology, The University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Lett 326:155-60. 2012
    ..In this review, we highlight recent advances in studies of the oncogenic role of Jab1/CSN5 in NPC and its potential as a therapeutic target for this cancer...
  63. pmc Taking the plunge: integrating structural, enzymatic and computational insights into a unified model for membrane-immersed rhomboid proteolysis
    Sinisa Urban
    Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochem J 425:501-12. 2010
    ..Although far from complete, studies with GlpG currently offer the best prospect for achieving a thorough and sophisticated understanding of a simplified intramembrane protease...
  64. pmc Roles of COP9 signalosome in cancer
    Mong Hong Lee
    The University of Texas MD Anderson Cancer Center, Houston, TX, USA
    Cell Cycle 10:3057-66. 2011
    ..Here, we discuss the mechanisms by which some CSN subunits are involved in cancer to provide a much needed perspective regarding COP9 in cancer research, hoping that these insights will lay the groundwork for cancer intervention...
  65. pmc Endolysosomal proteolysis and its regulation
    Ché S Pillay
    School of Molecular and Cellular Biosciences, University of Natal, Post Bag X01, Scottsville 3209, Pietermaritzburg, South Africa
    Biochem J 363:417-29. 2002
    ..Such systems would permit simultaneous regulation of a number of unrelated hydrolases...
  66. ncbi Co-localization of cortactin and phosphotyrosine identifies active invadopodia in human breast cancer cells
    Emma T Bowden
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, DC 20057 1469, USA
    Exp Cell Res 312:1240-53. 2006
    ..Thus, invadopodia, but not filopodia, are highly correlated with matrix invasion, and sites of invadopodial activity can be identified by the formation of invadopodial complexes...
  67. ncbi D1-protein dynamics in photosystem II: the lingering enigma
    Marvin Edelman
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel
    Photosynth Res 98:609-20. 2008
    ..This review will touch on some treaded areas of D1 research that have, so far, defied clear resolution, as well as cutting edge research on mechanisms and consequences of D1 protein degradation...
  68. ncbi The axial channel of the proteasome core particle is gated by the Rpt2 ATPase and controls both substrate entry and product release
    A Kohler
    Department of Cell Biology, Harvard Medical School, 02115, Boston, MA, USA
    Mol Cell 7:1143-52. 2001
    ..These experiments demonstrate that gating of the RP channel controls both substrate entry and product release, and is specifically regulated by an ATPase in the base of the RP...
  69. pmc Protease-sensitive conformers in broad spectrum of distinct PrPSc structures in sporadic Creutzfeldt-Jakob disease are indicator of progression rate
    Chae Kim
    Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    PLoS Pathog 7:e1002242. 2011
    ..The link between disease duration, levels, and stability of protease-sensitive conformers of PrP(Sc) suggests that these conformers play an important role in the pathogenesis of sCJD...
  70. ncbi The anaphase-promoting complex: proteolysis in mitosis and beyond
    Jan Michael Peters
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:931-43. 2002
    ....
  71. pmc Structural and kinetic determinants of protease substrates
    John C Timmer
    Apoptosis and Cell Death Research Program at the Burnham Institute for Medical Research, La Jolla, California, USA
    Nat Struct Mol Biol 16:1101-8. 2009
    ..This unique combination of proteomics, biochemistry, kinetics and substrate engineering reveals new insights into the structure-function relationship of protease targets and their validation from large-scale approaches...
  72. ncbi Atherosclerosis and proteinase activation
    Clare M Dollery
    Centre for Cardiovascular Biology and Medicine Division of Medicine, Rayne Institute 5 University Street, London, WC1E 6JJ, UK
    Cardiovasc Res 69:625-35. 2006
    ..This review weighs the evidence for the role of MMPs in arterial biology with particular reference to their activation in the atherosclerotic plaque...
  73. pmc PMAP: databases for analyzing proteolytic events and pathways
    Yoshinobu Igarashi
    The Center on Proteolytic Pathways, The Cancer Research Center and The Inflammatory and Infectious Disease Center, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 37:D611-8. 2009
    ..Here, we describe how the databases of PMAP can be used to foster understanding of proteolytic pathways, and equally as significant, to reason about proteolysis...
  74. ncbi Understanding the role of tissue degrading enzymes and their inhibitors in development and disease
    Tim E Cawston
    Musculoskeletal Research Group, 4th Floor Cookson Building, The Medical School, University of Newcastle upon Tyne, NE2 4HH, UK
    Best Pract Res Clin Rheumatol 20:983-1002. 2006
    ..It is likely that combinations of therapy will be required to ensure that joint destruction is prevented in arthritis patients...
  75. ncbi COP9 signalosome subunits 4 and 5 regulate multiple pleiotropic pathways in Drosophila melanogaster
    Efrat Oron
    Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Development 129:4399-409. 2002
    ..Both mutants have defective oocyte and embryo patterning, and defects in response to DNA damage, while csn5 mutants develop melanotic tumors and csn4 mutants have phenotypes reminiscent of defects in ecdysone signaling...
  76. pmc Amidoligases with ATP-grasp, glutamine synthetase-like and acetyltransferase-like domains: synthesis of novel metabolites and peptide modifications of proteins
    Lakshminarayan M Iyer
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Mol Biosyst 5:1636-60. 2009
    ..We present an evolutionary scenario for the multiple convergent origins of peptide ligases in various folds and clarify the bacterial origin of eukaryotic peptide-tagging enzymes of the TTL family...
  77. ncbi Diverse regulatory roles for lysosomal proteases in the immune response
    Jeff D Colbert
    Division of Cell Biology and Immunology, College of Life Sciences, University of Dundee, Dundee, UK
    Eur J Immunol 39:2955-65. 2009
    ..We pay attention to reports demonstrating cathepsin activity outside of its canonical endosome/lysosome microenvironment...
  78. pmc The Degradome database: mammalian proteases and diseases of proteolysis
    Victor Quesada
    Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain
    Nucleic Acids Res 37:D239-43. 2009
    ..Finally, the user can find additional information about protease structures, protease inhibitors, ancillary domains of proteases and differences between mammalian degradomes...
  79. pmc Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases
    Emilia S Olson
    Department of Pharmacology, Howard Hughes Medical Institute, Medical Scientist Training Program, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 107:4311-6. 2010
    ..These results should improve MRI-guided clinical staging, presurgical planning, and intraoperative fluorescence-guided surgery. The approach may be generalizable to deliver radiation-sensitizing and chemotherapeutic agents...
  80. ncbi SitePredicting the cleavage of proteinase substrates
    Jelle Verspurten
    Department for Molecular Biomedical Research, Flanders Institute for Biotechnology, Ghent, Belgium
    Trends Biochem Sci 34:319-23. 2009
    ..Comparison of the different programs shows that SitePrediction makes it possible to derive more reliable predictions. In addition, this tool allows the use of a wide range of proteinases...
  81. ncbi The COP9 signalosome and its role in plant development
    Claus Schwechheimer
    Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, Tubingen, Germany
    Eur J Cell Biol 89:157-62. 2010
    ..This review provides an update on recent advances in understanding CSN structure and function and summarises the current knowledge on its role in plant development and cell cycle progression...
  82. ncbi PNA-encoded protease substrate microarrays
    Nicolas Winssinger
    Institut de Science et d Ingenierie Supramoleculaires, Universite Louis Pasteur, 8 allée Guaspard Monge, 67000 Strasbourg, France
    Chem Biol 11:1351-60. 2004
    ..The methodology and validation of this approach for profiling proteolytic activity from single proteases and from those in crude cell lysates as well as clinical blood samples is described...
  83. ncbi Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii
    José de Jesús Serrano-Luna
    Departamento de Biologia Celular, CINVESTAV IPN, Mexico, D F Mexico
    Can J Microbiol 52:16-23. 2006
    ..polyphaga. Furthermore, aprotinin was found to inhibit crude extract protease activity on Madin-Darby canine kidney (MDCK) monolayers. These data suggest that protease patterns could be more complex than previously reported...
  84. pmc A statistics-based platform for quantitative N-terminome analysis and identification of protease cleavage products
    Ulrich auf dem Keller
    Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, 4 401 Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 9:912-27. 2010
    ....
  85. pmc Structural analysis of a viral ovarian tumor domain protease from the Crimean-Congo hemorrhagic fever virus in complex with covalently bonded ubiquitin
    Glenn C Capodagli
    Department of Chemistry and Biochemistry, University of Denver, 2190 E Iliff Ave, Olin 202, Denver, CO 80208, USA
    J Virol 85:3621-30. 2011
    ....
  86. pmc Profiling constitutive proteolytic events in vivo
    John C Timmer
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem J 407:41-8. 2007
    ..Taken together, our results demonstrate how to rapidly distinguish real proteolysis that occurs in vivo from the predictions based on in vitro experiments...
  87. ncbi The role of presenilin cofactors in the gamma-secretase complex
    Nobumasa Takasugi
    Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo 113 0033, Japan
    Nature 422:438-41. 2003
    ..Thus, APH-1 stabilizes the presenilin holoprotein in the complex, whereas PEN-2 is required for endoproteolytic processing of presenilin and conferring gamma-secretase activity to the complex...
  88. ncbi The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells
    Darren F Seals
    Van Andel Research Institute, Grand Rapids, Michigan 49503, USA
    Cancer Cell 7:155-65. 2005
    ..Thus, Tks5/Fish appears to be required for podosome formation, for degradation of the extracellular matrix, and for invasion of some cancer cells...
  89. pmc Proteolytic degradation of human salivary MUC5B by dental biofilms
    Claes Wickström
    Department of Oral Biology, Faculty of Odontology, Malmo University, Malmo, Sweden
    Microbiology 155:2866-72. 2009
    ..Certain bacteria in supragingival dental plaque therefore cooperate as a consortium to proteolyse human salivary MUC5B and hydrolyse glycosides...
  90. pmc Deletion mutants in COP9/signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes
    Kirsten E Mundt
    Genome Damage and Stability Centre, School of Biological Sciences, University of Sussex, Falmer, Sussex, BN1 9RR, United Kingdom
    Mol Biol Cell 13:493-502. 2002
    ..Our data suggest that the subunits of the signalosome cannot be considered as a distinct functional unit and imply that different subunits of the signalosome mediate distinct functions...
  91. pmc A Trojan horse mechanism of bacterial pathogenesis against nematodes
    Qiuhong Niu
    Laboratory for Conservation and Utilization of Bio Resources, Yunnan University, Kunming, Yunnan 650091, People s Republic of China
    Proc Natl Acad Sci U S A 107:16631-6. 2010
    ..This Trojan horse pattern of bacterium-nematode interaction enriches our understanding of microbial pathogenesis...
  92. pmc A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14
    A Borodovsky
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 20:5187-96. 2001
    ..Remarkably, labeling of 26S-associated USP14 with [(125)I]UbVS is increased when proteasome function is impaired, suggesting functional coupling between the activities of USP14 and the proteasome...
  93. ncbi The cell biology of leukocyte-mediated proteolysis
    C A Owen
    Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, USA
    J Leukoc Biol 65:137-50. 1999
    ..Recent studies, however, have identified several mechanisms by which inflammatory cells can degrade extracellular proteins in a milieu that contains high-affinity proteinase inhibitors...
  94. pmc Ca2+ activation of diffusible and bound pools of mu-calpain in rat skeletal muscle
    Robyn M Murphy
    Department of Zoology, La Trobe University, Bundoora Campus, Melbourne, Victoria 3086, Australia
    J Physiol 576:595-612. 2006
    ....
  95. pmc Phytaspase, a relocalisable cell death promoting plant protease with caspase specificity
    Nina V Chichkova
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    EMBO J 29:1149-61. 2010
    ..Phytaspase is constitutively secreted into the apoplast before PCD, but unexpectedly is re-imported into the cell during PCD providing insights into how phytaspase operates...
  96. pmc PrtT-regulated proteins secreted by Aspergillus fumigatus activate MAPK signaling in exposed A549 lung cells leading to necrotic cell death
    Haim Sharon
    Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel
    PLoS ONE 6:e17509. 2011
    ..These results suggest that inhibition of MAPK-mediated host responses to treatment with A. fumigatus CF decreases cellular damage, a finding with possible clinical implications...
  97. ncbi Identification and partial characterization of an elastolytic protease in the amphibian pathogen Batrachochytrium dendrobatidis
    A S Moss
    Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409 3131, USA
    Dis Aquat Organ 92:149-58. 2010
    ..The proteolytic activity exhibited in vitro might aid the organism in its ability to colonize and destroy the epidermis of its amphibian host...
  98. ncbi Characterization of digestive proteolytic activity in Lygus hesperus Knight (Hemiptera: Miridae)
    M Knop Wright
    USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S Providence Road, Columbia, MO 65203, USA
    J Insect Physiol 52:717-28. 2006
    ..Collectively, the caseinolytic activities complement one another, expanding the location and pH range over which digestion can occur...
  99. ncbi Methods for mapping protease specificity
    Scott L Diamond
    Penn Center for Molecular Discovery, Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, 1024 Vagelos Research Laboratories, University of Pennsylvania, Philadelphia PA 19104, USA
    Curr Opin Chem Biol 11:46-51. 2007
    ..These new methods continue to inform the design of chemical inhibitors and the identification of substrates of proteases...
  100. ncbi The conserved N-terminal helix of acylpeptide hydrolase from archaeon Aeropyrum pernix K1 is important for its hyperthermophilic activity
    Zuoming Zhang
    Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, PR China
    Biochim Biophys Acta 1784:1176-83. 2008
    ..Since the N-terminal helix is conserved in this family of enzymes, our results suggest that the N-terminal helix is likely to play an important role for stabilizing all other enzymes in this family...
  101. ncbi The COP9 signalosome mediates beta-catenin degradation by deneddylation and blocks adenomatous polyposis coli destruction via USP15
    Xiaohua Huang
    Department of General, Visceral, Vascular and Thoracic Surgery, Charite Universitatsmedizin Berlin, Germany
    J Mol Biol 391:691-702. 2009
    ..A model is provided that proposes a role of CSN-mediated deneddylation in the formation of the beta-catenin-degrading supercomplex and the protection of complex-bound APC via CSN-associated USP15...

Research Grants69

  1. INTESTINAL PEPTIDE HYDROLASES AND PEPTIDE TRANSPORT
    Young Kim; Fiscal Year: 2000
    ..Cellular changes in the expression of ACE and DPPIV along the crypt-villus axis during intestinal adaptation will be monitored by in situ hybridization and immunohistochemistry. ..
  2. The Penn Center For Molecular Discovery
    Scott Diamond; Fiscal Year: 2008
    ..This project is responsive to the NIH Roadmap objectives to apply both establish HTS/Synthetic methods and emerging next generation technologies developed at the University of Pennsylvania. ..
  3. Novel Roles for ADAM15 in Acute Lung Injury
    Caroline A Owen; Fiscal Year: 2010
    ..abstract_text> ..
  4. Positional cloning of a gene for mental retardation
    Joseph Higgins; Fiscal Year: 2004
    ..abstract_text> ..
  5. Multiplexed HTS of Serine and Cysteine Proteases (RMI)
    Scott Diamond; Fiscal Year: 2005
    ..The use of multiplexed assay on microarrays allows the generation of critical data for the chemical protease interactome. ..
  6. Blood Systems Biology
    Scott Diamond; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  7. Novel Roles for MMP-8 in Acute Lung Injury
    Caroline Owen; Fiscal Year: 2008
    ..We anticipate that in the future, our studies will facilitate the development of new treatment strategies that may reduce mortality and morbidity in ALl syndromes. ..
  8. Proteomics/Genomics of Opiate Analgesia and Addiction
    Vivian Hook; Fiscal Year: 2004
    ..Elucidation of distinct pathways will be significant, since it could allow future design of opiate drug regimens that provide effective analgesia, without the tolerance and dependence of addiction. ..
  9. Reaction and Transport Dynamics in Human Blood
    Scott Diamond; Fiscal Year: 2009
    ..Overall, these studies seek to provide fundamental insight into cell-cell interacitions and coagulation biochemistry that occur under flow. ..
  10. Role of cockroach proteases in airway inflammation
    Kristen Page; Fiscal Year: 2009
    ..Finally, we will investigate the role of endogenous TNF alpha in the airways using a TNF alpha neutralizing antibody and TNF alpha knockout mice. ..
  11. Mast Cells and IBD Pathogenesis
    LAURA HALE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  12. POSITIONAL CLONING OF A GENE FOR ESSENTIAL TREMOR
    Joseph Higgins; Fiscal Year: 2004
    ..abstract_text> ..
  13. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
    ..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. [unreadable] [unreadable] [unreadable]..
  14. Regulation of Vascular Function by Progesterone Receptor
    Luisa Iruela Arispe; Fiscal Year: 2007
    ..abstract_text> ..
  15. Gene networks specifying cell lineages in a polychaete
    Bruce Bowerman; Fiscal Year: 2007
    ..Newly discovered and conserved loci will also be studied in C. elegans and Drosophila. [unreadable] [unreadable]..
  16. Beta-Amyloid Protofibrils Cause AD Neuronal Dysfunction
    Dean Hartley; Fiscal Year: 2006
    ..abstract_text> ..
  17. Osteolysis and Implant Wear: Biological, Biomedical Engineering, and Surgical Pri
    Stuart Goodman; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  18. Influence of Heme on Hepatic Cytochrome P450 Synthesis and Degradation
    Maria Correia; Fiscal Year: 2007
    ..These studies are expected to elucidate how heme can affect the birth and death of these P450 proteins and thus modulate the effects and elimination of ingested drugs and environmental agents in man. ..
  19. Liver Injury in Alpha-1-Antitrypsin Deficiency
    David Perlmutter; Fiscal Year: 2007
    ..Thus, the studies proposed in this competitive renewal application will address the novel concept that oxidative stress causes, or contributes to, liver cell injury in alpha-1-ATZ deficiency. ..
  20. Targeting Cysteine Proteases: Antiparasitic Chemotherapy
    James McKerrow; Fiscal Year: 2008
    ..No Abstract Provided. ..
  21. Chemoprevention of IBD-Associated Colon Cancer
    Laura P Hale; Fiscal Year: 2010
    ..The proposed studies will further elucidate mechanisms that can lead to novel interventions to prevent IBD-associated colon cancers, providing great benefit to IBD patients and their families. ..
  22. Tumor and PTK-Targeted Therapy by FUS1 and PTK inhibitor
    Lin Ji; Fiscal Year: 2010
    ....
  23. MECHANISMS INVOLVED IN THE SEPTIC SHOCK SYNDROME
    Nilofer Qureshi; Fiscal Year: 2010
    ....
  24. Chlamydial Manipulation of Host Apoptosis
    Guangming Zhong; Fiscal Year: 2010
    ..abstract_text> ..
  25. Vein wall remodeling after DVT is matrix metalloproteinase dependent
    Peter Henke; Fiscal Year: 2009
    ..This proposal will provide important mechanistic insight into the pathophysiology of post-phelbitic syndrome with real potential translation to decreasing the morbidity from this under-acknowledged disease. ..
  26. RasGRP4-dependent Responses in Mast Cells
    Richard Stevens; Fiscal Year: 2009
    ..It also is anticipated that targeted disruption of RasGRP4 expression and/or activity in humans using pharmaceutical and other approaches will be beneficial in MC-dependent disorders. ..
  27. MMP-2 in Periodontal Disease and Oral Cancer
    Bjorn Steffensen; Fiscal Year: 2008
    ..The proposed studies should define the specific binding sites of MMP-2 and collagen and explore a new strategy to inhibit MMP-2 in periodontal disease and oral cancer. ..
  28. Comparative Evolutionary Genomics and Infectious Disease
    Jane Carlton; Fiscal Year: 2010
    ....
  29. Mucin Gene Regulation by Elastase and Oxidants
    Judith Voynow; Fiscal Year: 2008
    ..b. To determine whether NE and/or NQO1-generated ROS affect HuR binding to MUC5AC. c. To determine whether HuR binding is sufficient for MUC5AC mRNA stabilization. ..
  30. Screening for Genetic Mechanisms for Biliary Atresia
    David Perlmutter; Fiscal Year: 2006
    ..These studies will provide new information about and a basis for many additional studies of the pathogenesis, natural history, early diagnosis and treatment of BA, a rare and severe liver disease of infants ..
  31. AMELOGENIN PROCESSING AND APATITE CRYSTAL GROWTH
    JANET OLDAK; Fiscal Year: 2002
    ....
  32. PROTEIN SYNTHESIS INITIATION IN ANIMAL CELLS
    Bansidhar Datta; Fiscal Year: 2002
    ..This research is expected to provide valuable information regarding the mechanism of protein synthesis initiation in normal, cancerous, and virus- infected animal cells. ..
  33. REGULATION OF HYALURONAN EXPRESSION DURING REPAIR
    Dorne Yager; Fiscal Year: 2003
    ..abstract_text> ..
  34. Conference on Photodynamic Therapy
    David Kessel; Fiscal Year: 2002
    ..The most recent PDT meeting, sponsored by the International Photodynamic Assn, has more than 600 participants. ..
  35. MECHANISMS OF ATP-DEPENDENT PROTEOLYTIC ENZYMES
    Alfred Goldberg; Fiscal Year: 2004
    ..We also hope to learn more about this trimming process and about the competing pathway by which most proteasome products are digested to amino acids. ..
  36. Bromelain Proteinase Activity in IBD
    LAURA HALE; Fiscal Year: 2006
    ..They will thus strengthen the scientific knowledge base needed to inform the practice of complementary and alternative medicine. ..
  37. INNATE INTRAEPITHELIAL DEFENSE AGAINST ORAL INFECTION
    MARK HERZBERG; Fiscal Year: 2006
    ..abstract_text> ..
  38. CORTICOTROPIN RELEASING HORMONE INDUCED DURA MAST CELL A
    Theoharis Theoharides; Fiscal Year: 2001
    ..They may also help explain some key events in the pathophysiology of migraines, the clinical symptoms of which are often precipitated by stress. ..
  39. Protein Nitration in Retinal Light Damage
    Masaru Miyagi; Fiscal Year: 2004
    ..Results will reveal protein targets of nitration and proteins up and down regulated by light exposure. The results will lead to a better understanding of the mechanisms associated with retinal light damage. ..
  40. Angiogenesis and Microcirculation Gordon Conference
    Luisa Iruela Arispe; Fiscal Year: 2005
    ..The conference will be held in Salve Regina University from August 10-15, 2003. ..
  41. Serpin Protease Inhibitors & Opioid Neuropeptides
    Vivian Hook; Fiscal Year: 2006
    ..Serpin regulation of opioid peptides that mediate analgesia and stress may lead to development of new therapeutic approaches in pain and neurologic disease. ..
  42. Regulation and Proteomics of Polyubiquitin Signaling
    ERIC COOPER; Fiscal Year: 2008
    ..Specifically, the goal is to establish a paradigm for how these chains are recognized, what factors bind to them, and how such signals are ultimately turned off. ..
  43. Targeting Allosteric Studies to Modulate Protein Interactions and Function
    Charles Craik; Fiscal Year: 2009
    ....
  44. KINETICS AND MECHANISM OF THE HEAT SHOCK 70 PROTEIN DNAK
    STEPHAN WITT; Fiscal Year: 2002
    ..abstract_text> ..
  45. RANK Ligand is a Bone Anabolic Agent
    Steven Teitelbaum; Fiscal Year: 2006
    ..abstract_text> ..
  46. ROLE OF PRE FIBRILLAR AMYLOID IN ALZHEIMER'S DISEASE
    Dean Hartley; Fiscal Year: 2002
    ....
  47. Structural Basis of Serpin Function and Regulation
    Steven Olson; Fiscal Year: 2009
    ..The proposed studies will utilize mutagenesis, fluorescence, NMR and X-ray crystallography and thermodynamic and kinetic approaches to characterize serpin-protease and serpin-ligand interactions. ..
  48. MECHANISMS OF AUTOIMMUNE ARTHRITIS
    EDWARD ROSLONIEC; Fiscal Year: 2001
    ..These recombinant DR molecules will be tested for their ability to alter T-cell responses to hCII in vitro, and prevent the development of autoimmune arthritis in the DR transgenic mice. ..
  49. Genetic basis of P. aeruginosa lung infections in mice
    Hongwei Yu; Fiscal Year: 2004
    ..The discovery of the mouse susceptibility gene may lead to the identification of the human homologue (lung-specific modifier gene) presumed to mediate susceptibility in the initial phase of infection. ..
  50. INTEGRATED DNA SEQUENCING SYSTEM
    Vivian Hook; Fiscal Year: 2002
    ..abstract_text> ..
  51. Evaluating Trichophyton tonsurans Carriage and Infection
    Susan Abdel Rahman; Fiscal Year: 2006
    ..abstract_text> ..
  52. Magnetic Resonance Imaging of Alzheimer's Amyloid Plaque
    JOSEPH PODUSLO; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  53. PARVOVIRUS STRUCTURE, CAPSID ASSEMBLY, AND RECEPTORS
    Colin Parrish; Fiscal Year: 2007
    ..abstract_text> ..
  54. Host Protein Degradation by Schistosome Parasites
    James McKerrow; Fiscal Year: 2007
    ..abstract_text> ..
  55. ROLE OF MYOSTATIN IN CACHEXIA
    Se Jin Lee; Fiscal Year: 2005
    ..abstract_text> ..
  56. INHIBITION OF THE ANGIOGENIC RESPONSE
    Luisa Iruela Arispe; Fiscal Year: 2006
    ..abstract_text> ..
  57. Cell Biology of Ion Pumps: Sorting and Function
    Michael Caplan; Fiscal Year: 2004
    ..These studies will allow us to determine whether and how specific molecular signals and associations might be related to such clinically significant pathologies as gastric ulcer disease and hypertension. ..
  58. LOCAL EXPRESSION OF ALPHA-1-ANTITRYPSIN IN EMPHYSEMA
    David Perlmutter; Fiscal Year: 2008
    ....
  59. Cortactin in HNSSC tumor progression
    ALISSA WEAVER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  60. In vivo panning for schistosome protective epitopes
    Charles Shoemaker; Fiscal Year: 2005
    ....
  61. Gene Therapy of Muscle Wasting From Uremia
    Xiaonan Wang; Fiscal Year: 2005
    ..We believe that the experimental results will shed the insight on the mechanisms of muscle protein degradation, and provide a possible new approach to treat the pathological process of muscle wasting. ..
  62. Intracellular mycobacterial proteome
    Qingbo Li; Fiscal Year: 2008
    ..They may be important for understanding the physiology of intracellular persistence of mycobacteria within different macrophages. ..
  63. Antibody Immunotherapy to a Pro-apoptotic Tumor Antigen
    Kenneth Rock; Fiscal Year: 2004
    ..In another set of experiments, we will test the effect of p40 on human tumors transplanted into immunodeficient mice. ..
  64. DIMERIZATION INHIBITORS OF HIV-1 PROTEASE AND INTEGRASE
    Jean Chmielewski; Fiscal Year: 2003
    ..complex? Integrase Inhibition How general is the approach developed for dimerization inhibition of HIV protease, can it be applied to the inhibition of a structurally different protein such as HIV integrase? ..
  65. DEGRADATION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2008
    ..abstract_text> ..
  66. Trypanosomatid Mitochondrial DNA Polymerases
    Michele Klingbeil; Fiscal Year: 2008
    ..The T. brucei pol I-like protein family also offers the unique opportunity to uncover insights into the evolutionary strategies used to produce a replicative polymerase. ..
  67. CARDIOVASCULAR LUNG, AND BLOOD IMMUNOBIOLOGY IN HEALTH
    David Corry; Fiscal Year: 2004
    ..Together, these data will elucidate an epithelium- dependent immunomodulatory mechanism of the lung mediated through CCSP, and potentially identify novel means for the therapy of allergic lung disorders such as asthma...