carbonic anhydrases

Summary

Summary: A family of zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide. They play an important role in the transport of CARBON DIOXIDE from the tissues to the LUNG. EC 4.2.1.1.

Top Publications

  1. ncbi Carbonic anhydrases: novel therapeutic applications for inhibitors and activators
    Claudiu T Supuran
    Laboratorio di Chimica Bioinorganica, Universita degli Studi di Firenze, Rm 188, Via della Lastruccia 3, I 50019 Sesto Fiorentino, Firenze, Italy
    Nat Rev Drug Discov 7:168-81. 2008
  2. ncbi Regulation of tumor pH and the role of carbonic anhydrase 9
    Pawel Swietach
    Department of Physiology, Anatomy and Genetics, Burdon Sanderson Cardiac Science Centre, Oxford OX1 3PT, UK
    Cancer Metastasis Rev 26:299-310. 2007
  3. ncbi Hypoxia inducible carbonic anhydrase IX, marker of tumour hypoxia, survival pathway and therapy target
    Christian Potter
    Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital Oxford, UK
    Cell Cycle 3:164-7. 2004
  4. ncbi Carbonic anhydrase IX is an independent predictor of survival in advanced renal clear cell carcinoma: implications for prognosis and therapy
    Matthew H T Bui
    Department of Urology, University of California, Los Angeles, California 90095 1738, USA
    Clin Cancer Res 9:802-11. 2003
  5. ncbi Carbonic anhydrase IX in renal cell carcinoma: implications for prognosis, diagnosis, and therapy
    Alexander B Stillebroer
    Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Eur Urol 58:75-83. 2010
  6. ncbi The carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata
    Hiroshi Miyamoto
    Faculty of Biology Oriented Science and Technology, Department of Molecular Genetics, Kinki University, Uchita, Wakayama, Japan
    Zoolog Sci 22:311-5. 2005
  7. ncbi CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution
    Mario Giordano
    Department of Marine Sciences, Universita Politecnica delle Marche, 60121 Ancona, Italy
    Annu Rev Plant Biol 56:99-131. 2005
  8. pmc Transcriptional control of the tumor- and hypoxia-marker carbonic anhydrase 9: A one transcription factor (HIF-1) show?
    Stefan Kaluz
    Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697 4025, USA
    Biochim Biophys Acta 1795:162-72. 2009
  9. pmc Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer
    S Ivanov
    Science Applications International Corporation, Laboratory of Immunobiology, Bldg 560, NCI FCRDC, P O Box B, Frederick, MD 21702, USA
    Am J Pathol 158:905-19. 2001
  10. ncbi Sensitivity to 6-n-propylthiouracil is associated with gustin (carbonic anhydrase VI) gene polymorphism, salivary zinc, and body mass index in humans
    Alessandra Padiglia
    Department of Science Applied to Biosystems, University of Cagliari, Monserrato, Italy
    Am J Clin Nutr 92:539-45. 2010

Detail Information

Publications340 found, 100 shown here

  1. ncbi Carbonic anhydrases: novel therapeutic applications for inhibitors and activators
    Claudiu T Supuran
    Laboratorio di Chimica Bioinorganica, Universita degli Studi di Firenze, Rm 188, Via della Lastruccia 3, I 50019 Sesto Fiorentino, Firenze, Italy
    Nat Rev Drug Discov 7:168-81. 2008
    b>Carbonic anhydrases (CAs), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, including gluconeogenesis, lipogenesis, ureagenesis, tumorigenicity and the growth and ..
  2. ncbi Regulation of tumor pH and the role of carbonic anhydrase 9
    Pawel Swietach
    Department of Physiology, Anatomy and Genetics, Burdon Sanderson Cardiac Science Centre, Oxford OX1 3PT, UK
    Cancer Metastasis Rev 26:299-310. 2007
    ..We also review recent evidence that may suggest a role for CA9 in coordinating pH(i) among cells of large, unvascularized cell-clusters...
  3. ncbi Hypoxia inducible carbonic anhydrase IX, marker of tumour hypoxia, survival pathway and therapy target
    Christian Potter
    Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital Oxford, UK
    Cell Cycle 3:164-7. 2004
    ..b>Carbonic anhydrases have a widespread role in normal tissues in regulating pH, with 14 isoforms described, so inhibition may ..
  4. ncbi Carbonic anhydrase IX is an independent predictor of survival in advanced renal clear cell carcinoma: implications for prognosis and therapy
    Matthew H T Bui
    Department of Urology, University of California, Los Angeles, California 90095 1738, USA
    Clin Cancer Res 9:802-11. 2003
    ..To date, there are no molecular markers which can reliably predict RCC outcome. We investigated whether a novel kidney cancer marker, carbonic anhydrase IX (CAIX), is associated with progression and survival...
  5. ncbi Carbonic anhydrase IX in renal cell carcinoma: implications for prognosis, diagnosis, and therapy
    Alexander B Stillebroer
    Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Eur Urol 58:75-83. 2010
    ..The clinical management of patients with renal cell carcinoma (RCC) remains difficult, and the development of new diagnostic, prognostic, and therapeutic tools is still required...
  6. ncbi The carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata
    Hiroshi Miyamoto
    Faculty of Biology Oriented Science and Technology, Department of Molecular Genetics, Kinki University, Uchita, Wakayama, Japan
    Zoolog Sci 22:311-5. 2005
    ..Together these studies suggest that nacrein functions as a negative regulator in calcification in the extrapallial space between the shell and the mantle by inhibiting the precipitation of CaCO3...
  7. ncbi CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution
    Mario Giordano
    Department of Marine Sciences, Universita Politecnica delle Marche, 60121 Ancona, Italy
    Annu Rev Plant Biol 56:99-131. 2005
    ..We discuss the diversity of CCMs, their evolutionary origins, and the role of the environment in CCM modulation...
  8. pmc Transcriptional control of the tumor- and hypoxia-marker carbonic anhydrase 9: A one transcription factor (HIF-1) show?
    Stefan Kaluz
    Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697 4025, USA
    Biochim Biophys Acta 1795:162-72. 2009
    ..In conclusion, the existing data support the notion that CA9, due to the unique structure of its promoter, is one of the most sensitive endogenous sensors of HIF-1 activity...
  9. pmc Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer
    S Ivanov
    Science Applications International Corporation, Laboratory of Immunobiology, Bldg 560, NCI FCRDC, P O Box B, Frederick, MD 21702, USA
    Am J Pathol 158:905-19. 2001
    ..We conclude that the cell surface trans-membrane carbonic anhydrases CA IX and CA XII are overexpressed in many tumors suggesting that this is a common feature of cancer cells ..
  10. ncbi Sensitivity to 6-n-propylthiouracil is associated with gustin (carbonic anhydrase VI) gene polymorphism, salivary zinc, and body mass index in humans
    Alessandra Padiglia
    Department of Science Applied to Biosystems, University of Cagliari, Monserrato, Italy
    Am J Clin Nutr 92:539-45. 2010
    ....
  11. ncbi Cancer-associated carbonic anhydrases and their inhibition
    S Pastorekova
    Center of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
    Curr Pharm Des 14:685-98. 2008
    ..This review summarizes research data related to distribution, regulation and functional aspects of CA IX and CA XII, and describes emerging possibilities for clinical exploitation of CA inhibitors as imaging tools and anticancer drugs...
  12. ncbi High stromal carbonic anhydrase IX expression is associated with nodal metastasis and decreased survival in patients with surgically-treated oral cavity squamous cell carcinoma
    Nigel T Brockton
    Division of Preventive Oncology, Department of Oncology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Oral Oncol 48:615-22. 2012
    ..Assessment of stromal CAIX expression could identify patients with the least favorable prognosis and inform therapeutic strategies in OCSCC...
  13. ncbi Polymorphisms in TAS2R38 and the taste bud trophic factor, gustin gene co-operate in modulating PROP taste phenotype
    Carla Calo
    Department of Experimental Biology, Section of Anthropology, Monserrato, CA, I 09042, Italy
    Physiol Behav 104:1065-71. 2011
    ..These data show how the combination of the TAS2R38 and gustin gene genotypes modulate PROP phenotype, providing an additional tool for the evaluation of human eating behavior and nutritional status...
  14. ncbi Evaluating the role of carbonic anhydrases in the transport of HCO3--related species
    Walter F Boron
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106 4970, USA
    Biochim Biophys Acta 1804:410-21. 2010
    ..Thus, it appears that CAII does not bind directly to AE1 or other SLC4 proteins, and that bound CAII does not substantially accelerate HCO(3)(-)-initiated HCO(3)(-) transport...
  15. ncbi In vivo targeting of tumor-associated carbonic anhydrases using acetazolamide derivatives
    Julia K J Ahlskog
    Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, ETH Zurich, CH 8093 Zurich, Switzerland
    Bioorg Med Chem Lett 19:4851-6. 2009
    ....
  16. ncbi Overexpression of carbonic anhydrase IX (CAIX) is an independent unfavorable prognostic marker in endometrioid ovarian cancer
    Matthias Choschzick
    Institute of Pathology, University Medical Centre Hamburg Eppendorf, Hamburg, Germany
    Virchows Arch 459:193-200. 2011
    ..Our data support the potential therapeutic benefit of newly developed targeting antibodies in advanced ovarian cancer...
  17. ncbi Imaging of CA IX with fluorescent labelled sulfonamides distinguishes hypoxic and (re)-oxygenated cells in a xenograft tumour model
    Ludwig Dubois
    Maastricht Radiation Oncology MaastRO Lab, GROW School for Oncology and Developmental Biology, University of Maastricht, The Netherlands
    Radiother Oncol 92:423-8. 2009
    ..The aim of this study was to investigate the in vivo CAI binding properties in a xenograft tumour model using fluorescent imaging...
  18. ncbi Serum carbonic anhydrase IX during first-line therapy of ovarian cancer
    Linn Woelber
    Department of Gynecology, University Medical Center Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Gynecol Oncol 117:183-8. 2010
    ..It is overexpressed in a variety of tumors including ovarian cancer. To evaluate the potential prognostic and predictive role of serum CAIX for therapy response in ovarian cancer, we analyzed longitudinal serum samples...
  19. ncbi Pimonidazole binding and tumor vascularity predict for treatment outcome in head and neck cancer
    Johannes H A M Kaanders
    Department of Radiation Oncology, University Medical Center Nijmegen, 6500 HB Nijmegen, The Netherlands
    Cancer Res 62:7066-74. 2002
    ..Pimonidazole and CA9 demonstrate concordant staining patterns, but the latter is a less specific marker for hypoxia...
  20. ncbi Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH
    Eliska Svastova
    Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
    FEBS Lett 577:439-45. 2004
    ..Our findings suggest that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression...
  21. pmc The key hypoxia regulated gene CAIX is upregulated in basal-like breast tumours and is associated with resistance to chemotherapy
    E Y Tan
    Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, UK
    Br J Cancer 100:405-11. 2009
    ....
  22. ncbi Expression of hypoxia-inducible carbonic anhydrase-9 relates to angiogenic pathways and independently to poor outcome in non-small cell lung cancer
    A Giatromanolaki
    Tumour and Angiogenesis Research Group, Department of Radiotherapy Oncology, Democritus University of Thrace, Alexandroupolis 68100, Greece
    Cancer Res 61:7992-8. 2001
    ..The expression of CA9 is linked to the expression of a constellation of proteins involved in angiogenesis, apoptosis inhibition, and cell-cell adhesion disruption, which explains the strong association of CA9 with poor outcome...
  23. ncbi Colocalization of carbonic anhydrase 9 expression and cell proliferation in human head and neck squamous cell carcinoma
    Ilse J Hoogsteen
    Department of Radiation Oncology, University Medical Center, St Radboud, Nijmegen, The Netherlands
    Clin Cancer Res 11:97-106. 2005
    ..Colocalization of IdUrd and CA9 may identify an important subpopulation of tumor cells that might be responsible for repopulation and disease progression...
  24. ncbi Carbonic anhydrase IX reduces E-cadherin-mediated adhesion of MDCK cells via interaction with beta-catenin
    Eliska Svastova
    Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
    Exp Cell Res 290:332-45. 2003
    ..We conclude that CA IX has a capacity to modulate E-cadherin-mediated cell adhesion via interaction with beta-catenin, which could be of potential significance in hypoxia-induced tumor progression...
  25. ncbi Cloning and characterization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment
    J Pastorek
    Institute of Virology, Slovak Academy of Sciences, Bratislava
    Oncogene 9:2877-88. 1994
    ..amino acid sequence revealed strong structural homology between the central region of the MN protein and carbonic anhydrases (CA). MN sequence retains the conserved zinc-binding site as well as the enzyme's active center...
  26. ncbi Endogenous markers of two separate hypoxia response pathways (hypoxia inducible factor 2 alpha and carbonic anhydrase 9) are associated with radiotherapy failure in head and neck cancer patients recruited in the CHART randomized trial
    Michael I Koukourakis
    Department of Radiotherapy Oncology, Democritus University of Thrace, Alexandroupolis, Greece
    J Clin Oncol 24:727-35. 2006
    ....
  27. ncbi Coexpression of hypoxia-inducible factors 1alpha and 2alpha, carbonic anhydrase IX, and vascular endothelial growth factor in nasopharyngeal carcinoma and relationship to survival
    Edwin P Hui
    Departments of Clinical Oncology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong
    Clin Cancer Res 8:2595-604. 2002
    ....
  28. ncbi Role of carbonic anhydrase IX in human tumor cell growth, survival, and invasion
    Naomi Robertson
    Cancer Research UK, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom
    Cancer Res 64:6160-5. 2004
    ..Thus, CAIX plays an important role in the growth and survival of tumor cells under normoxia and hypoxia, making it a potential target for cancer therapy, but is not involved in invasion...
  29. ncbi Assessment of tumour hypoxia for prediction of response to therapy and cancer prognosis
    Adrian M Jubb
    Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
    J Cell Mol Med 14:18-29. 2010
    ..This review aims to discuss the clinical impact of translational work on hypoxia markers and to explore future directions for research in this area...
  30. pmc N-ethyl-N-nitrosourea-induced null mutation at the mouse Car-2 locus: an animal model for human carbonic anhydrase II deficiency syndrome
    S E Lewis
    Research Triangle Institute, NC 27709
    Proc Natl Acad Sci U S A 85:1962-6. 1988
    ..The CA II protein, which is normally expressed in most tissues, could not be detected by immunodiffusion analysis in any tissues of the CA II-deficient mice, suggesting a nonsense or a missense mutation at the Car-2 locus...
  31. ncbi A validated gene expression profile for detecting clinical outcome in breast cancer using artificial neural networks
    L J Lancashire
    Clinical and Experimental Pharmacology, Paterson Institute for Cancer Research, University of Manchester, Manchester, M20 4BX, UK
    Breast Cancer Res Treat 120:83-93. 2010
    ..Importantly our principal prognosticator, CA9, showed that it is capable of selecting an aggressive subgroup of patients who are known to have poor prognosis...
  32. pmc DNA damage is a prerequisite for p53-mediated proteasomal degradation of HIF-1alpha in hypoxic cells and downregulation of the hypoxia marker carbonic anhydrase IX
    Milota Kaluzova
    Department of Microbiology and Molecular Genetics, University of California at Irvine, College of Medicine, Irvine 92717, USA
    Mol Cell Biol 24:5757-66. 2004
    ..A model outlining the quantitative relationship between p53, HIF-1alpha, and CAIX is presented...
  33. pmc Responsiveness to 6-n-propylthiouracil (PROP) is associated with salivary levels of two specific basic proline-rich proteins in humans
    Tiziana Cabras
    Department of Life and Environment Sciences, Macrosection of Biomedicine, University of Cagliari, Monserrato, Cagliari, Italy
    PLoS ONE 7:e30962. 2012
    ..These findings suggest that PRB1, in addition to TAS2R38 and gustin, could contribute to individual differences in thiourea sensitivity, and the expression of the PROP phenotype as a complex genetic trait...
  34. ncbi Fibrotic focus and hypoxia in male breast cancer
    Robert Kornegoor
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    Mod Pathol 25:1397-404. 2012
    ..These markers seem to have similar clinical importance as previously reported in female breast cancer...
  35. ncbi Prognostic value of carbonic anhydrase IX and KI67 as predictors of survival for renal clear cell carcinoma
    Matthew H T Bui
    Departments of Urology, University of California Los Angeles, California 90095 1738, USA
    J Urol 171:2461-6. 2004
    ..The natural history of renal cell carcinoma (RCC) is complex and not entirely explained by conventional prognostic factors. In this study we evaluated the prognostic value of carbonic anhydrase IX (CAIX) and Ki67 to predict survival in RCC...
  36. ncbi Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target
    Giuseppina De Simone
    Istituto di Biostrutture e Bioimmagini CNR, 80134 Naples, Italy
    Biochim Biophys Acta 1804:404-9. 2010
    ..As a consequence, CA IX represents a promising antitumor target for the development of anticancer agents with an alternative mechanism of action...
  37. ncbi Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization
    Aurélie Moya
    Centre Scientifique de Monaco, Avenue Saint Martin, Principality of Monaco
    J Biol Chem 283:25475-84. 2008
    b>Carbonic anhydrases (CA) play an important role in biomineralization from invertebrates to vertebrates. Previous experiments have investigated the role of CA in coral calcification, mainly by pharmacological approaches...
  38. ncbi Effect of distributional heterogeneity on the analysis of tumor hypoxia based on carbonic anhydrase IX
    Vladimir V Iakovlev
    Ontario Cancer Institute, Princess Margaret Hospital, Toronto, ON, Canada
    Lab Invest 87:1206-17. 2007
    ..However, CAIX was inferior to pO2 measurements as a tool for patient stratification. Improved analytical methods to account for intratumoral heterogeneity are needed to provide reliable measurements of molecular markers...
  39. ncbi Carbonic anhydrases II and XII are up-regulated in osteoclast-like cells in advanced human atherosclerotic plaques-Tampere Vascular Study
    Niku Oksala
    Centre for Laboratory Medicine, Tampere University Hospital and Department of Clinical Chemistry, Medical School, University of Tampere, Tampere, Finland
    Ann Med 42:360-70. 2010
    b>Carbonic anhydrases (CA) play a central role in osteoclast function and bone remodeling by catalyzing the formation of bicarbonate and proton from carbon dioxide...
  40. ncbi Regulatory mechanism underlying cyclic changes in mouse uterine bicarbonate secretion: role of estrogen
    Qiong He
    Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, People s Republic of China
    Reproduction 140:903-10. 2010
    ....
  41. ncbi Investigations of the esterase, phosphatase, and sulfatase activities of the cytosolic mammalian carbonic anhydrase isoforms I, II, and XIII with 4-nitrophenyl esters as substrates
    Alessio Innocenti
    Universita degli Studi di Firenze, Laboratorio di Chimica Bioinorganica, Rm 188, Via della Lastruccia 3, I 50019 Sesto Fiorentino, Firenze, Italy
    Bioorg Med Chem Lett 18:2267-71. 2008
    ..CA XIII was the most effective esterase and phosphatase. CA XIII might catalyze other physiological reactions than CO(2) hydration, based on its relevant phosphatase activity...
  42. ncbi Biochemistry: a cadmium enzyme from a marine diatom
    Todd W Lane
    Biosystems Research Department, Sandia National Laboratories, Livermore, California 94551 0969, USA
    Nature 435:42. 2005
    ....
  43. ncbi Carbonic anhydrase 9 in clear cell renal cell carcinoma: a marker for diagnosis, prognosis and treatment
    Jacques Tostain
    Department of Urology Andrology, University Jean Monnet, Teaching Hospital, Saint Etienne, France
    Eur J Cancer 46:3141-8. 2010
    ..Targeting CA9 by inhibitors, radioimmunotherapy, monoclonal antibodies or vaccination is promising and offers new avenues for clinical research...
  44. ncbi Docking, synthesis, and in vitro evaluation of antimitotic estrone analogs
    Andre Stander
    Department of Physiology, University of Pretoria, Pretoria, South Africa
    Chem Biol Drug Des 77:173-81. 2011
    ....
  45. pmc Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence
    Torsten Klengel
    Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    Curr Biol 15:2021-6. 2005
    ..Novel therapeutic agents could target this pathway at several levels to control fungal infections...
  46. ncbi Biomarkers predicting outcome in patients with advanced renal cell carcinoma: Results from sorafenib phase III Treatment Approaches in Renal Cancer Global Evaluation Trial
    Carol Pena
    Bayer HealthCare Pharmaceuticals, Montville, New Jersey 07045 1000, USA
    Clin Cancer Res 16:4853-63. 2010
    ....
  47. ncbi The catalytic properties of human carbonic anhydrase IX
    T Wingo
    Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610 0267, USA
    Biochem Biophys Res Commun 288:666-9. 2001
    Human carbonic anhydrase IX (CA IX) is an integral membrane protein and a member of the alpha class of carbonic anhydrases that includes the human and animal enzymes...
  48. pmc Prognostic relevance of carbonic anhydrase-IX in high-risk, early-stage cervical cancer: a Gynecologic Oncology Group study
    Shu Yuan Liao
    Department of Epidemiology, University of California at Irvine, School of Medicine, Irvine, CA, USA
    Gynecol Oncol 116:452-8. 2010
    ....
  49. pmc High levels of carbonic anhydrase IX in tumour tissue and plasma are biomarkers of poor prognostic in patients with non-small cell lung cancer
    M Ilie
    Laboratory of Clinical and Experimental Pathology, Louis Pasteur Hospital, Nice, France
    Br J Cancer 102:1627-35. 2010
    ..This study was conducted to assess if the expression of CAIX in tumour tissue and/or plasma can be a prognostic factor in patients with non-small cell lung cancer (NSCLC)...
  50. ncbi Hypoxia and metabolic phenotypes during breast carcinogenesis: expression of HIF-1alpha, GLUT1, and CAIX
    Chi long Chen
    Department of Pathology, College of Medicine, Taipei Medical University, Taipei, Taiwan
    Virchows Arch 457:53-61. 2010
    ..GLUT1 expression (glycolytic phenotype) and CAIX expression (acid-resistant phenotype) may result in a powerful adaptive advantage and represent an aggressive phenotype...
  51. pmc Expression of the hypoxia-inducible and tumor-associated carbonic anhydrases in ductal carcinoma in situ of the breast
    C C Wykoff
    Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom
    Am J Pathol 158:1011-9. 2001
    b>Carbonic anhydrases (CA) influence intra- and extracellular pH and ion transport in varied biological processes. We recently identified CA9 and CA12 as hypoxia-inducible genes...
  52. ncbi Carbonic anhydrases as targets for medicinal chemistry
    Claudiu T Supuran
    Universita degli Studi di Firenze, Laboratorio di Chimica Bioinorganica, Rm 188, Via della Lastruccia 3, I 50019 Sesto Fiorentino Firenze, Italy
    Bioorg Med Chem 15:4336-50. 2007
    b>Carbonic anhydrases (CAs, EC 4.2.1.1) are zinc enzymes acting as efficient catalysts for the reversible hydration of carbon dioxide to bicarbonate...
  53. ncbi New insights into the physiological role of carbonic anhydrase IX in tumour pH regulation
    P Swietach
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    Oncogene 29:6509-21. 2010
    ..This is now an important avenue for further investigation. The importance of CAIX in regulating tumour pH highlights the protein as a potential target for cancer therapy...
  54. ncbi Carbonic anhydrase IX as an anticancer therapy target: preclinical evaluation of internalizing monoclonal antibody directed to catalytic domain
    M Zatovicova
    Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, Slovak Republic
    Curr Pharm Des 16:3255-63. 2010
    ..These have not been explored so far and include broad spectrum of cancer types, treatment of which might benefit from CA IX-mediated targeting...
  55. ncbi Carbonic anhydrase IX expression, hypoxia, and prognosis in patients with uterine cervical carcinomas
    David Hedley
    Division of Experimental Therapeutics and Department of Radiation Oncology, Ontario Cancer Institute Princess Margaret Hospital, Toronto, Ontario, Canada
    Clin Cancer Res 9:5666-74. 2003
    ..Recent studies suggest that carbonic anhydrase IX (CA IX), which is regulated via hypoxia-inducible factor 1, is a useful intrinsic marker of tumor hypoxia...
  56. ncbi Targeting tumor hypoxia: suppression of breast tumor growth and metastasis by novel carbonic anhydrase IX inhibitors
    Yuanmei Lou
    Department of Integrative Oncology, BC Cancer Agency, British Columbia, Canada
    Cancer Res 71:3364-76. 2011
    ..Our findings show that CAIX is vital for growth and metastasis of hypoxic breast tumors and is a specific, targetable biomarker for breast cancer metastasis...
  57. pmc IL-6 triggers malignant features in mammospheres from human ductal breast carcinoma and normal mammary gland
    Pasquale Sansone
    Center for Applied Biomedical Research, St Orsola Malpighi University Hospital, Department of Pharmacology and Toxicology, University of Bologna, Bologna, Italy
    J Clin Invest 117:3988-4002. 2007
    ..In conclusion, these data support the hypothesis that IL-6 induces malignant features in Notch-3-expressing stem/progenitor cells from human ductal breast carcinoma and normal mammary gland...
  58. pmc Efficiency of the CO2-concentrating mechanism of diatoms
    Brian M Hopkinson
    Department of Geosciences, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 108:3830-7. 2011
    ..To achieve the CO(2) concentration necessary to saturate carbon fixation, the CO(2) is most likely concentrated within the pyrenoid, an organelle within the chloroplast where the CO(2)-fixating enzyme is located...
  59. ncbi Necrosis and hypoxia in invasive breast carcinoma
    Ladislav Tomes
    Faculty of Medicine, Department of Pathology, University of Manitoba, Winnipeg, MB, Canada
    Breast Cancer Res Treat 81:61-9. 2003
    ..We conclude that stromal and epithelial hypoxia may have different biological backgrounds and that stromal hypoxia may affect survival...
  60. ncbi Tumor hypoxia at the micro-regional level: clinical relevance and predictive value of exogenous and endogenous hypoxic cell markers
    Johan Bussink
    Department of Radiation Oncology, University Medical Center Nijmegen, 6500 HB, The, Nijmegen, Netherlands
    Radiother Oncol 67:3-15. 2003
    ....
  61. pmc Expression of vascular endothelial growth factor, hypoxia inducible factor 1alpha, and carbonic anhydrase IX in human tumours
    A M Jubb
    Department of Pathology, Genentech Inc, South San Francisco, CA94080, USA
    J Clin Pathol 57:504-12. 2004
    ....
  62. pmc Carbon dioxide transport through membranes
    Andreas Missner
    Institut für Biophysik and Institut für Industriemathematik, Johannes Kepler Universitat, Altenbergerstrasse 69, Linz, Austria
    J Biol Chem 283:25340-7. 2008
    ..The conclusion was confirmed by the observation that CO(2) permeability of epithelial cell monolayers was always the same whether AQP1 was overexpressed in both the apical and basolateral membranes or not...
  63. ncbi Biphasic and dually coordinated expression of the genes encoding major shell matrix proteins in the pearl oyster Pinctada fucata
    Takeshi Takeuchi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1 1 1, Tsukuba, 305 8572, Japan
    Mar Biotechnol (NY) 8:52-61. 2006
    ....
  64. ncbi Carbonic anhydrase IX in bladder cancer: a diagnostic, prognostic, and therapeutic molecular marker
    Tobias Klatte
    Department of Urology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095 1738, USA
    Cancer 115:1448-58. 2009
    ..The objective of this study was to evaluate the role of carbonic anhydrase IX (CAIX) in urothelial carcinoma of the bladder...
  65. doi Molecular mechanisms of carbonic anhydrase IX-mediated pH regulation under hypoxia
    Silvia Pastorekova
    Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    BJU Int 101:8-15. 2008
  66. pmc The role of carbonic anhydrase 9 in regulating extracellular and intracellular ph in three-dimensional tumor cell growths
    Pawel Swietach
    From the Department of Physiology, Anatomy and Genetics, Burdon Sanderson Cardiac Science Centre, Oxford OX1 3PT, United Kingdom
    J Biol Chem 284:20299-310. 2009
    ..The results suggest a possible basis for attenuating tumor development through inhibiting CA9 activity...
  67. ncbi Tumor-associated carbonic anhydrase 9 spatially coordinates intracellular pH in three-dimensional multicellular growths
    Pawel Swietach
    Department of Physiology, Anatomy, and Genetics, Burdon Sanderson Cardiac Science Centre, Oxford, UK
    J Biol Chem 283:20473-83. 2008
    ..We suggest that pH(i) coordination may favor survival and growth of a tumor. By disrupting spatial pH(i) control, inhibition of CA9 activity may offer a novel strategy for the clinical treatment of CA9-associated tumors...
  68. pmc Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients
    J Zavada
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nam 2, 16637 Prague, Czech Republic
    Br J Cancer 89:1067-71. 2003
    ..After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals...
  69. ncbi Proteins of calcified endoskeleton: II partial amino acid sequences of endoskeletal proteins and the characterization of proteinaceous organic matrix of spicules from the alcyonarian, Synularia polydactyla
    M Azizur Rahman
    Department of Marine and Environmental Sciences, University of the Ryukyus, Okinawa, Japan
    Proteomics 5:885-93. 2005
    ..These findings suggest that the endoskeletal spicules of alcyonarian corals have protein-rich organic matrices, which might be related to the calcification process...
  70. pmc The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response
    David H Slaymaker
    Waksman Institute, Rutgers, State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 99:11640-5. 2002
    ..These results demonstrate that SA may act through multiple effector proteins in plants and shed further light on the function of CA in chloroplasts...
  71. ncbi Physiology and molecular biology of renal carbonic anhydrase
    George J Schwartz
    Pediatric Nephrology, University of Rochester School of Medicine, NY 14642, USA
    J Nephrol 15:S61-74. 2002
    ..To establish other roles for CA in the kidney will require further kinetic, functional, immunolocalization and cloning studies...
  72. ncbi Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast
    Arsenio Villarejo
    Umea Plant Science Centre, Department of Plant Physiology, Umea University, S 90187 Umea, Sweden
    Nat Cell Biol 7:1224-31. 2005
    ..Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast...
  73. ncbi Lowered oxygen tension induces expression of the hypoxia marker MN/carbonic anhydrase IX in the absence of hypoxia-inducible factor 1 alpha stabilization: a role for phosphatidylinositol 3'-kinase
    Stefan Kaluz
    Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Cancer Res 62:4469-77. 2002
    ..These interdependent pathways function at a lowered O(2) concentration that is, however, above that necessary for HIF-1 alpha stabilization...
  74. ncbi Glycosyl coumarin carbonic anhydrase IX and XII inhibitors strongly attenuate the growth of primary breast tumors
    Nadia Touisni
    Institut des Biomolecules Max Mousseron IBMM, UMR 5247 CNRS UM1 UM2, Bâtiment de Recherche Max Mousseron, Ecole Nationale Superieure de Chimie de Montpellier, 8 rue de l Ecole Normale, 34296 Montpellier Cedex, France
    J Med Chem 54:8271-7. 2011
    ..Because CA IX is overexpressed in hypoxic tumors and exhibits very limited expression in normal tissues, such compounds may be useful for treating cancers not responsive to classic chemo- and radiotherapy...
  75. ncbi Carbonic anhydrase IX as a target for metastatic disease
    Roben G Gieling
    Hypoxia and Therapeutics Group, School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Stopford Building, Oxford Road, Rm 3 125, M13 9PL Manchester, United Kingdom
    Bioorg Med Chem 21:1470-6. 2013
    ..Studies in tumour xenograft models showed that these CA IX-specific inhibitors and antibodies can be very effective in therapy and imaging of a variety of different metastatic cancers...
  76. ncbi The presence of a fibrotic focus in invasive breast carcinoma correlates with the expression of carbonic anhydrase IX and is a marker of hypoxia and poor prognosis
    Cecile G Colpaert
    Department of Pathology, University Hospital Antwerp, University of Antwerp, Edegem, Belgium
    Breast Cancer Res Treat 81:137-47. 2003
    ..FF is useful as a surrogate marker of hypoxia-driven ongoing angiogenesis and is associated with a higher relapse rate and a worse overall survival...
  77. ncbi The response of nutrient assimilation and biochemical composition of Arctic seaweeds to a nutrient input in summer
    Francisco J L Gordillo
    Departamento de Ecologia, Facultad de Ciencias, Universidad de Malaga, Campus Teatinos, E 29071 Malaga, Spain
    J Exp Bot 57:2661-71. 2006
    ..This indicates a high degree of resilience of the algal community to a disruption in the natural nutrient availability pattern...
  78. ncbi Structure and catalytic mechanism of the beta-carbonic anhydrases
    Roger S Rowlett
    Colgate University, Department of Chemistry, Hamilton, NY 13346, USA
    Biochim Biophys Acta 1804:362-73. 2010
    The beta-carbonic anhydrases (beta-CAs) are a diverse but structurally related group of zinc-metalloenzymes found in eubacteria, plant chloroplasts, red and green algae, and in the Archaea...
  79. ncbi Carbonic anhydrase in calcified endoskeleton: novel activity in biocalcification in alcyonarian
    M Azizur Rahman
    Department of Marine and Environmental Sciences, University of the Ryukyus, Okinawa, Japan
    Mar Biotechnol (NY) 10:31-8. 2008
    ..Our observations strongly suggest that the matrix protein in alcyonarian coral is not only a structural protein but also a catalyst...
  80. ncbi Expression of carbonic anhydrase IX in breast is associated with malignant tissues and is related to overexpression of c-erbB2
    Maria Bartosova
    Department of Molecular Biology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    J Pathol 197:314-21. 2002
    ..02). No significant association was found with the other markers. The results of this study support the possible importance of CA IX for breast carcinogenesis and suggest its potential use as a breast tumour marker...
  81. ncbi Carbonic anhydrases in anthozoan corals-A review
    Anthony Bertucci
    Centre Scientifique de Monaco, Avenue Saint Martin, MC 98000, Monaco
    Bioorg Med Chem 21:1437-50. 2013
    ..b>Carbonic anhydrases play major roles in those two essential processes of coral's physiology: they are involved in the carbon ..
  82. pmc S0859, an N-cyanosulphonamide inhibitor of sodium-bicarbonate cotransport in the heart
    F F T Ch'en
    Burdon Sanderson Cardiac Science Centre, Department of Physiology, Anatomy and Genetics, Division of Medical Sciences, Oxford University, Oxford, UK
    Br J Pharmacol 153:972-82. 2008
    ..Compound S0859 (C(29)H(24)ClN(3)O(3)S) is a putative NBC inhibitor. Here, we provide the drug's chemical structure, test its potency and selectivity in ventricular cells and assess its suitability for experiments on cardiac contraction...
  83. ncbi Targeting tumor-associated carbonic anhydrase IX in cancer therapy
    Anne Thiry
    Drug Design and Discovery Center, University of Namur, 61 rue de Bruxelles, B 5000 Namur, Belgium
    Trends Pharmacol Sci 27:566-73. 2006
    ..Indeed, fluorescent inhibitors and membrane-impermeant sulfonamides have recently been used as proof-of-concept tools, demonstrating that CA IX is an interesting target for anticancer drug development...
  84. ncbi Nacre calcification in the freshwater mussel Unio pictorum: carbonic anhydrase activity and purification of a 95 kDa calcium-binding glycoprotein
    Benjamin Marie
    UMR CNRS 5561 Biogeosciences, Universite de Bourgogne, Dijon, France
    Chembiochem 9:2515-23. 2008
    ..Partial primary structures were obtained by de novo sequencing and compared with known sequences from other mollusc shell proteins. Our results are discussed from an evolutionary viewpoint...
  85. ncbi Phosphorylation of carbonic anhydrase IX controls its ability to mediate extracellular acidification in hypoxic tumors
    Peter Ditte
    Department of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Cancer Res 71:7558-67. 2011
    ..Our findings indicate that CA IX is a pivotal mediator of the hypoxia-cAMP-PKA axis, which regulates pH in the hypoxic tumor microenvironment...
  86. ncbi The role of carbonic anhydrases in renal physiology
    J M Purkerson
    Department of Pediatrics, University of Rochester School of Medicine, Rochester, New York, USA
    Kidney Int 71:103-15. 2007
    ..For example, CAII on the cytoplasmic face and CAIV on the extracellular surface provide the 'push' and 'pull' for bicarbonate transport by supplying and dissipating substrate respectively...
  87. ncbi CA9 gene: single nucleotide polymorphism predicts metastatic renal cell carcinoma prognosis
    Michela de Martino
    Department of Urology, University of California Los Angeles, Los Angeles, California 90095 1738, USA
    J Urol 182:728-34. 2009
    ..The CA9 gene exons and flanking regions were amplified by polymerase chain reaction and sequenced. The monoclonal antibody M75 was used to evaluate CAIX protein expression in the primary tumor by immunohistochemistry...
  88. ncbi Prokaryotic carbonic anhydrases
    K S Smith
    Department of Biochemistry and Molecular Biology, 204 South Frear Laboratory, The Pennsylvania State University, University Park, PA 16802, USA
    FEMS Microbiol Rev 24:335-66. 2000
    b>Carbonic anhydrases catalyze the reversible hydration of CO(2) [CO(2)+H(2)Oright harpoon over left harpoon HCO(3)(-)+H(+)]...
  89. ncbi Expression of transmembrane carbonic anhydrase isoenzymes IX and XII in normal human pancreas and pancreatic tumours
    A J Kivela
    Department of Anatomy and Cell Biology, University of Oulu, Finland
    Histochem Cell Biol 114:197-204. 2000
    ..However, the increased expression of CA IX in hyperplastic ductal epithelium may contribute to the pancreatic tumourigenesis...
  90. pmc A novel alpha-type carbonic anhydrase associated with the thylakoid membrane in Chlamydomonas reinhardtii is required for growth at ambient CO2
    J Karlsson
    Department of Plant Physiology, Umea University, S 901 87 Umea, Sweden
    EMBO J 17:1208-16. 1998
    ..reinhardtii at ambient levels of CO2, and that at these CO2 concentrations the enzyme is required for optimal photosystem II photochemistry...
  91. ncbi Carbonic anhydrase II is an AP-1 target gene in osteoclasts
    J P David
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Physiol 188:89-97. 2001
    ..Thus, our data demonstrate that cFos/AP-1 can directly regulate the expression of this osteoclast marker and that AP-1 activity is upregulated in osteoclast progenitors in response to osteoclastogenic signals...
  92. ncbi Inhibitory effects of cadmium on carbonic anhydrase activity and ionic regulation of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae)
    A M Vitale
    Biological Sciences Department, University of Buenos Aires, Argentina
    Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 122:121-9. 1999
    ..15 x 10(-5), 1.62 x 10(-5), 3.75 x 10(-6) and 4.4 x 10(-10) M for cadmium, copper, zinc and AZ, respectively. The comparison with IC50 values of other aquatic species, indicates a higher CA sensitivity for C. granulata to pollutants...
  93. ncbi The carbonic anhydrase isoforms of Chlamydomonas reinhardtii: intracellular location, expression, and physiological roles
    James V Moroney
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Photosynth Res 109:133-49. 2011
    ..b>Carbonic anhydrases (CAs) are important components of the CCM...
  94. pmc The tumour-associated carbonic anhydrases CA II, CA IX and CA XII in a group of medulloblastomas and supratentorial primitive neuroectodermal tumours: an association of CA IX with poor prognosis
    Kristiina Nordfors
    Department of Pathology, Tampere University Hospital, Tampere, Finland
    BMC Cancer 10:148. 2010
    ..Our aim was to investigate whether carbonic anhydrases (CAs), enzymes commonly overexpressed in various tumours including glioblastomas and oligodendrogliomas, ..
  95. pmc Expression and activity of carbonic anhydrase IX is associated with metabolic dysfunction in MDA-MB-231 breast cancer cells
    Ying Li
    Department of Biochemistry and Molecular Biology, University of Florida, College of Medicine, Gainesville, Florida 32610, USA
    Cancer Invest 27:613-23. 2009
    ..Together, these studies suggest that CAIX expression and activity is associated with metabolic dysfunction in MDA-MB-231 cells...
  96. pmc Design of a carbonic anhydrase IX active-site mimic to screen inhibitors for possible anticancer properties
    Caroli Genis
    Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Biochemistry 48:1322-31. 2009
    ..This preliminary study demonstrates that the CA IX mimic may provide a useful model to design more isozyme-specific CA IX inhibitors, which may lead to development of new therapeutic treatments of some cancers...
  97. pmc Exploring local flexibility/rigidity in psychrophilic and mesophilic carbonic anhydrases
    R Chiuri
    National Nanotechnology Laboratory of CNR INFM, IIT Research Unit, Lecce, Italy
    Biophys J 96:1586-96. 2009
    ....
  98. pmc A biological function for cadmium in marine diatoms
    T W Lane
    Department of Geosciences, Princeton University, Princeton, NJ 08544 1003, USA
    Proc Natl Acad Sci U S A 97:4627-31. 2000
    ..It is now clear that T. weissflogii expresses a Cd-specific CA, which, particularly under conditions of Zn limitation, can replace the Zn enzyme TWCA1 in its carbon-concentrating mechanism...
  99. pmc Importance of post-translational modifications for functionality of a chloroplast-localized carbonic anhydrase (CAH1) in Arabidopsis thaliana
    Stefan Burén
    Department of Plant Physiology, Umea Plant Science Centre, Umea University, Umea, Sweden
    PLoS ONE 6:e21021. 2011
    ..Therefore, to elucidate the significance of glycosylation in trafficking and the effect of glycosylation on the stability and function of the protein, epitope-labelled wild type and mutated versions of CAH1 were expressed in plant cells...
  100. ncbi Targeting hypoxic tumor cell viability with carbohydrate-based carbonic anhydrase IX and XII inhibitors
    Jason C Morris
    Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Queensland 4111, Australia
    J Med Chem 54:6905-18. 2011
    ..Our findings identified two inhibitors (compounds 5 and 17) that block CA IX-induced survival and have potential for development as in vivo cancer cell selective inhibitors...
  101. ncbi Reduced expression of hypoxia-inducible factor-1alpha in perinecrotic regions of solid tumors
    Solmaz Sobhanifar
    British Columbia Cancer Research Center, Vancouver, British Columbia, Canada
    Cancer Res 65:7259-66. 2005
    ..These results have implications for the use of HIF-1alpha as an indicator of tumor hypoxia and aggressiveness as well as development of hypoxia-directed antitumor therapies based on the expression of HIF-1alpha...

Research Grants62

  1. Mitochondrial Carbonic Anhydrases and Diabetic Blood-Brain Barrier Disruption
    GUL NASREEN SHAH; Fiscal Year: 2013
    ..Mitochondrial carbonic anhydrases (CAs) are important in the generation of reactive oxygen species (ROS) and the subsequent oxidative stress ..
  2. Dynamics of pH Regulation in the Brain
    Mitchell Chesler; Fiscal Year: 2013
    ..Studies will make use of animals with a knockout of specific carbonic anhydrases, and will utilize transgenic mice with green fluorescence protein expression in specific interneuron ..
  3. Chelator Fragment Libraries for Optimizing Metal-Ligand Interactions in Metallopr
    Seth M Cohen; Fiscal Year: 2013
    ..studies on two specific Zn2+dependent systems (Aim 2, Aim 3), the matrix metalloproteinases (MMPs) and carbonic anhydrases (CAs)...
  4. Directed Evolution of Metal-Substituted Carbonic Anhydrases for Catalysis
    LEVI MICHAEL STANLEY; Fiscal Year: 2013
    ..The first aim is focused on the identification of metal-substituted carbonic anhydrases that exhibit activity as catalysts for C-H functionalization reactions...
  5. Carbonic anhydrase and pH control in breast cancer cells
    Susan Cooke Frost; Fiscal Year: 2013
    ..Our overall goal is to understand the role of membrane-bound carbonic anhydrases, whose catalytic domains face the extracellular milieu, in controlling acidification in breast cancer...
  6. CATALYTIC MECHANISM OF CARBONIC ANHYDRASE
    David N Silverman; Fiscal Year: 2012
    ..The unifying goal of this application is to expand our use of the carbonic anhydrases to understand such rate-limiting and long-range proton transfer steps in a way that can be extended to ..
  7. SITE SPECIFIC MUTAGENESIS OF H PYLORI CARBONIC ANHYDRASE
    ROGER ROWLETT; Fiscal Year: 1999
    ..from the Applicant's Abstract) Helicobacter pylori, a human gastric pathogen, codes for two different carbonic anhydrases in its genome, both an alpha and beta form...
  8. MUTAGENESIS TO PROBE CATALYSIS BY CARBONIC ANHYDRASE II
    Carol Fierke; Fiscal Year: 1992
    ..Information gained will impact on our understanding of the mechanism of carbonic anhydrases and other metalloenzymes, as well as the mechanism of proton transfers in all proteins...
  9. STRUCTURE/FUNCTION OF CARBONIC ANHYDRASE
    David Christianson; Fiscal Year: 2001
    DESCRIPTION: (adapted from applicant's abstract) The zinc-requiring carbonic anhydrases (CA) are ubiquitous throughout the plant, animal, and bacterial kingdoms, and no other family of enzymes characterized by such catalytic, cellular, ..
  10. SECOND INTERNATIONAL NOMENCLATURE WORKSHOP
    Muriel Davisson; Fiscal Year: 1999
    ..various species (vertebrates, invertebrates, yeast, plants), gene families (calcium channels, aquaporins, carbonic anhydrases, annexins), and databases (MGI, GDB, NCBI, SWISS-PROT) will discuss nomenclature issues such as 1) the need ..
  11. CARBONIC ANHYDRASE AND MYELIN DEVELOPMENT
    MARJORIE LEES; Fiscal Year: 1980
    ..Lees, M.B. and Koul, O.: Regional Development and Fractional Studies on Myelin and other carbonic anhydrases in rat CNS in Myelination and demyelination: Recent Chemical advances. Palo, J. and Riekennen, P., (Eds...
  12. Carbonic Anhydrases in Obesity and Insulin Resistance
    JACK YOUNGREN; Fiscal Year: 2003
    ..These studies should provide important new data, therefore, on the CA family of enzymes, and their role in the insulin resistance. ..
  13. Structural Aspects of Bicarbonate Transport Metabolons
    Peter Piermarini; Fiscal Year: 2005
    ..The HCO3-transporters NBCe1& AE1, and carbonic anhydrases CA II & CAIV are proteins that play important roles in HCO3-reabsorption by the renal tubule...
  14. VISION RESEARCH AT INDIANA UNIVERSITY
    Joseph Bonanno; Fiscal Year: 2008
    ..They include advanced optics of the eye, visual displays for infant vision development, microscopic fluorimetry and imaging, and equipment repair support. [unreadable] [unreadable] [unreadable]..
  15. ENZYMOLOGY OF ACETOTROPHIC METHANOGENESIS
    JAMES FERRY; Fiscal Year: 2003
    ..Three of these enzymes are virtually ubiquitous in anaerobes: acetate kinase, phosphotransacetylase, and carbonic anhydrases of the (and (classes...
  16. CURCUMIN AS AN INHIBITOR OF PROSTATE CANCER BONE METASTASIS
    Thambi Dorai; Fiscal Year: 2007
    ..Feasibility of this approach will offer prostate cancer patients a safe and nontoxic way to treat the metasatic complications at least in an adjuvant manner, along with other therapies. [unreadable] [unreadable] [unreadable]..
  17. Single Cell Analysis of Cross Talk Among Kinase Pathways
    Stefan Kaluz; Fiscal Year: 2008
    ..These studies will provide additional information relating to our understanding of the consequencesof activationof kinase signaling pathwayswith respect to malignant transformation, aggressive tumor growth and survival. ..
  18. ANION REGULATION IN LARVAE OF DISEASE VECTOR MOSQUITOES
    PAUL LINSER; Fiscal Year: 2009
    ..targeted as they have central roles in anion production and transport in the gut alkalinization process: carbonic anhydrases and transmembrane anion transporters...
  19. CORNEAL METABOLIC ACTIVITY IN HUMANS
    Joseph Bonanno; Fiscal Year: 2002
    ..If so, this could lead to the development of a provocative test for suitability of contact lens wear based on a quick and simple measure of corneal oxygen consumption. ..
  20. Defining Molecular Markers for Tumor Hypoxia
    QUYNH THU XUAN LE; Fiscal Year: 2010
    ..abstract_text> ..
  21. Metal Transfer Reactions between Stable Isotopically Doped Zn Proteins
    Andrew Mason; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  22. Statistical Methods in Diagnostic Medicine
    Xiao Hua Zhou; Fiscal Year: 2007
    ..Finally, we will develop general group sequential designs for comparative ROC studies using nonparametrically estimated ROC curves. [unreadable] [unreadable]..
  23. SALIVARY MUCOUS CELL GENE EXPRESSION
    DAVID CULP; Fiscal Year: 2007
    ..Moreover, expression of a novel human gene expressing a salivary apomucin will be verified, and if expressed, would be the focus of future studies to determine mechanisms regulating its expression. [unreadable] [unreadable]..
  24. MOLECULAR PHYSIOLOGY OF BAND 3 LIKE PROTEINS OF KIDNEY
    Seth Alper; Fiscal Year: 2007
    ..5. Define aspects of transcriptional and translational regulation of AE gene products in kidney of mutant and parental mouse strains and in cultured kidney cells. ..
  25. Therapeutic Modulation of COX-2-induced Immunosuppression in Metastatic RCC
    Brian Rini; Fiscal Year: 2008
    ..This project will have broad implications for the immunoregulation of cancer and how expression and modulation of COX-2 expression influences the immune response to cancer. [unreadable] [unreadable] [unreadable]..
  26. Apical CI/OH-/HCO3-exchanger in kidney and GI tract
    Manoocher Soleimani; Fiscal Year: 2007
    ..They should further facilitate future investigations into the role of this transporter in pathophysiologic states affecting electrolyte and acid-base homeostasis. ..
  27. Structural Investigation of Allosteric Regulation in Bacterial Carbonic Anhydrase
    JEFF CRONK; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): The carbonic anhydrases (CAs) catalyze a reaction of fundamental biochemical and physiological importance, the interconversion of carbon dioxide and bicarbonate ion CO2 + H2O - All CAs ..
  28. Elucidating Novel Topological Features in Protein Structures
    Todd O Yeates; Fiscal Year: 2010
    ..The proteins to be studied here possess unique features that make them valuable in efforts to achieve a fundamental understanding of protein structure and stability. ..
  29. Fluorescence Zinc BioSensing and Imaging Technology
    RICHARD BLAIR THOMPSON; Fiscal Year: 2010
    ..This novel technology will provide the basis for new diagnostics and treatments. ..
  30. Utility of [F-18] fluoroDOPA for neonatal hyperinsulism: A Phase II
    Chaitanya Divgi; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  31. BIOCHEMICAL GENETICS OF CARBONIC ANHYDRASE DEFICIENCIES
    William Sly; Fiscal Year: 2008
    ..The sixteen known carbonic anhydrases (CAs) and CA-related proteins play important roles in diverse physiological processes including respiration,..
  32. Oral Infectious Disease: Virulence and Host Determinants
    DAVID CULP; Fiscal Year: 2009
    ..Future studies can then focus on molecular mechanisms of host-pathogen interactions. Results may indicate important therapeutic targets to treat patients at high risk for caries (i.e., patients with xerostomia). ..
  33. Cyclotron for biomedical research
    Chaitanya Divgi; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  34. Defining Cell Lineages in the Bladder Urothelium
    Sabina Signoretti; Fiscal Year: 2006
    ..These studies will clarify lineage relationships among bladder epithelial cells and provide important insights into the nature of urothelial stem cells. ..
  35. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  36. Antiestrogenic Effects on Tumor Angiogenesis
    Kimberly Blackwell; Fiscal Year: 2006
    ..The results of this project will have profound implications, especially as clinicians begin to use the newer estrogen receptor modulating drugs for the prevention and treatment of breast cancer. ..
  37. Sickle Red Cell K+ Transporter Genetics in S. cerevisiae
    Seth Alper; Fiscal Year: 2002
    ..3) (Provisional Aim): Time permitting, to improve, validate, and standardize growth rescue of trk1delta/trk2delta S. cerevisiae by expression in nonpermissive conditions of cDNA encoding the mammalian IK1 K(ca) channel. ..
  38. GENE DEFECTIVE IN HEREDITARY HEMOCHROMATOSIS
    William Sly; Fiscal Year: 2002
    ..These studies will not only improve our understanding of how iron absorption is normally regulated, but may also suggest new strategies for treating disorders of excessive iron absorption. ..
  39. Fourier Transform Ion Cyclotron Resonance Mass Spect.
    Mehdi Moini; Fiscal Year: 2003
    ..3) Chemical Composition Analysis of Expanded Porphyrins. Some of these expanded porphyrins are used to develop drugs for diagnosis and treatment of human diseases such as radiation therapy enhancing agents. ..
  40. PHYSIOLOGY OF RENAL SODIUM/BICARBONATE COTRANSPORT
    Walter Boron; Fiscal Year: 2001
    ..The use of isolated, perfused tubules will insure that the expression of the transporters is as normal as possible, and that anatomical origin of the tubules is precisely known. ..
  41. SEXUAL DIMORPHISM IN SEIZURE CONTROL
    JANA VELISKOVA; Fiscal Year: 2002
    ..Rats of both sexes and various ages will be used. These studies may lead to the development of new antiepileptic treatments that take into the account sexual age-related differences. ..
  42. QUANTITATIVE IMAGING OF ZINC METABOLISM IN THE BRAIN
    Richard Thompson; Fiscal Year: 2002
    ..abstract_text> ..
  43. REGULATION OF COLONIC H-K-ATPASE IN THE KIDNEY
    Manoocher Soleimani; Fiscal Year: 2001
    ..Insight into regulation of cHKA will significantly enhance our knowledge on a variety of pathologic conditions associated with electrolyte and acid-base abnormalities. ..
  44. CHARACTERIZATION OF NA-DEPENDENT HCO3-TRANSPORTERS
    Manoocher Soleimani; Fiscal Year: 2001
    ..Insight into regulation and gene structure of Na-coupled HCO3 transporters should enhance our knowledge on cell regulation and acid-base homeostasis. ..
  45. REGULATION OF GLANDULAR MUCOUS CELL SECRETION
    DAVID CULP; Fiscal Year: 2001
    ..Results will provide specific information for determination of the dose and temporal administration of agonist in future clinical trials to treat patients with salivary hypofunction. ..
  46. QUANTITATION OF HYPOXIC TUMOR CELLS
    Peggy Olive; Fiscal Year: 2001
    ..To validate the models, expression of GFP will be analyzed in relation to proliferation, hypoxia marker binding, and clonogenicity following irradiation. ..
  47. PH REGULATION IN VENTRICULAR CELLS
    KENNETH SPITZER; Fiscal Year: 2002
    ..The results of this project will yield new information concerning hormonal modulation of cardiac pHi control systems and the action of intracellular and intercellular H+ diffusion to dissipate regional pHi gradients. ..
  48. Ultraview LCI Spinning Disk Confocal Microscope System
    Dennis Brown; Fiscal Year: 2003
    ..Thus, a wide-range of projects and investigators will benefit from this equipment, which will be integrated into a Core facility that has been operational for over 15 years. ..
  49. QUANTITATIVE IMAGING OF ZINC METABOLISM IN THE BRAIN
    Richard Thompson; Fiscal Year: 2003
    ..abstract_text> ..
  50. Evaluation of models of pharmacoresistant epilepsy.
    H White; Fiscal Year: 2005
    ..abstract_text> ..
  51. Physiological isoforms of sAC in male germ cells
    Jochen Buck; Fiscal Year: 2005
    ..A long-term objective of this work is development of highly specific pharmacological reagents targeting individual isoforms as a novel method of contraception or to treat infertility. ..
  52. ION CHANNEL REGULATION BY THE CYTOPLASMIC TAIL OF PDK1
    Seth Alper; Fiscal Year: 2005
    ..We will search for novel interacting proteins, and prepare protein suitable for structural analysis. ..
  53. JEOL JEM-1011 Transmission Electron Microscope
    Dennis Brown; Fiscal Year: 2005
    ..abstract_text> ..
  54. Polyamine metabolism in heart and kidney ischemic injury
    Manoocher Soleimani; Fiscal Year: 2005
    ..abstract_text> ..
  55. Prenatal Corticosteroid Impact on Hippocampus
    LIBOR VELISEK; Fiscal Year: 2004
    ..The data of this proposal will be critical for determining long-term adverse effects of multiple course prenatal corticosteroid therapy and for the ameliorating these effects in the offspring. ..
  56. Causal Analysis of Encouragement Design Studies
    Xiao Hua Zhou; Fiscal Year: 2004
    ..In this proposal, we will develop a general statistical methodology for causal treatment analysis of the randomized EDS when data have a complicated structure. ..
  57. Regression Analysis of Health Care Costs
    Xiao Hua Zhou; Fiscal Year: 2004
    ..Furthermore, contributions will be demonstrated in the area of health services research by applying the proposed methods to analyze of total health care costs in four existing datasets. ..
  58. METABOLIC AND TRANSPORT PATHWAYS IN SKELETAL MUSCLE
    Robert Forster; Fiscal Year: 2004
    ..4) Determine the facilitation of CO2 diffusion in a thin electrolyte film containing slow oxidative cytosol or CA III, in support of Hypothesis 3. ..
  59. CHIMERIC RNA/DNA OLIGONUCLEOTIDE BASED GENE THERAPY
    YEONG HAU LIEN; Fiscal Year: 2003
    ..This preclinical study will provide critical information for future development of optimal gene targeting therapy for treating hereditary renal diseases, such as autosomal dominant polycystic kidney disease. ..