carbonic anhydrase ii

Summary

Summary: A cytosolic carbonic anhydrase isoenzyme found widely distributed in cells of almost all tissues. Deficiencies of carbonic anhydrase II produce a syndrome characterized by OSTEOPETROSIS, renal tubular acidosis (ACIDOSIS, RENAL TUBULAR) and cerebral calcification. EC 4.2.1.-

Top Publications

  1. ncbi Carbonic anhydrase II binds to and enhances activity of the Na+/H+ exchanger
    Xiuju Li
    Department of Biochemistry and Physiology, Canadian Institute of Health Research Membrane Protein Group, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 277:36085-91. 2002
  2. pmc Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth
    Akari Inada
    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 105:19915-9. 2008
  3. ncbi A transport metabolon. Functional interaction of carbonic anhydrase II and chloride/bicarbonate exchangers
    D Sterling
    Membrane Transport Group and Canadian Institutes of Health Research Group in Molecular Biology of Membrane Proteins, Department of Physiology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 276:47886-94. 2001
  4. pmc Activity and distribution of intracellular carbonic anhydrase II and their effects on the transport activity of anion exchanger AE1/SLC4A1
    Samer Al-Samir
    G Gros Zentrum Physiologie, Vegetative Physiologie 4220, Medizinische Hochschule Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany V Endeward Zentrum Physiologie 4220, Medizinische Hochschule Hannover, Germany
    J Physiol 591:4963-82. 2013
  5. ncbi A novel carbonic anhydrase II binding site regulates NHE1 activity
    Xiuju Li
    Department of Biochemistry, 347 Medical Science Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochemistry 45:2414-24. 2006
  6. ncbi Selective hydrophobic pocket binding observed within the carbonic anhydrase II active site accommodate different 4-substituted-ureido-benzenesulfonamides and correlate to inhibitor potency
    Fabio Pacchiano
    Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Box 100245, Gainesville, Florida 32610, USA
    Chem Commun (Camb) 46:8371-3. 2010
  7. ncbi Carbonic anhydrase II promotes cardiomyocyte hypertrophy
    Brittany F Brown
    Membrane Protein Disease Research Group, Department of Biochemistry, School of Translational Medicine, University of Alberta, Edmonton, AB, Canada
    Can J Physiol Pharmacol 90:1599-610. 2012
  8. pmc Overexpression of carbonic anhydrase II and Ki-67 proteins in prognosis of gastrointestinal stromal tumors
    Li Cheng Liu
    Department of General Surgery, General Hospital of PLA, Beijing 100853, China
    World J Gastroenterol 19:2473-80. 2013
  9. pmc Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion
    Pia Johansson
    Department of Pharmacology, University of Melbourne, Parkville, Victoria 3010, Australia
    Neurosci Lett 442:77-80. 2008
  10. ncbi Timing and expression pattern of carbonic anhydrase II in pancreas
    Akari Inada
    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Dev Dyn 235:1571-7. 2006

Research Grants

  1. H+-ATPase B-subunit Dysfunction and Calcium Nephrolithiasis
    Orson W Moe; Fiscal Year: 2010
  2. Physiology of Electrogenic Na/HCO3 Cotransporters
    Walter F Boron; Fiscal Year: 2011
  3. CELLULAR BASIS OF CRANIOFACIAL BONE DISORDERS
    Philip Osdoby; Fiscal Year: 2002
  4. Supramolecular Approaches to Protein Inhibitors Activated by MicroRNA-21
    Janarthanan Jayawickramarajah; Fiscal Year: 2013
  5. REGULATION OF CARBONIC ANHYDRASE II GENE EXPRESSION
    LUCYNDIA MARINO; Fiscal Year: 1993
  6. MUTAGENESIS TO PROBE CATALYSIS BY CARBONIC ANHYDRASE II
    Carol Fierke; Fiscal Year: 1992
  7. INTERACTION OF SPIN LABELS WITH TRANSITION METALS
    GARETH EATON; Fiscal Year: 2002
  8. FOLDING OF CARBONIC ANHYDRASE
    Sandra Eaton; Fiscal Year: 1999
  9. CIRCULAR DICHROISM SPECTROMETER
    TERRENCE OAS; Fiscal Year: 1993
  10. Recombinant T-cell Receptor Ligands for Treatment of Uveitis
    Grazyna Adamus; Fiscal Year: 2006

Detail Information

Publications233 found, 100 shown here

  1. ncbi Carbonic anhydrase II binds to and enhances activity of the Na+/H+ exchanger
    Xiuju Li
    Department of Biochemistry and Physiology, Canadian Institute of Health Research Membrane Protein Group, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 277:36085-91. 2002
    We examined the ability of carbonic anhydrase II to bind to and affect the transport efficiency of the NHE1 isoform of the mammalian Na(+)/H(+) exchanger...
  2. pmc Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth
    Akari Inada
    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 105:19915-9. 2008
    ..progenitors after birth and give rise to new islets, we generated transgenic mice expressing human carbonic anhydrase II (CAII) promoter: Cre recombinase (Cre) or inducible CreER(TM) to cross with ROSA26 loxP-Stop-loxP LacZ ..
  3. ncbi A transport metabolon. Functional interaction of carbonic anhydrase II and chloride/bicarbonate exchangers
    D Sterling
    Membrane Transport Group and Canadian Institutes of Health Research Group in Molecular Biology of Membrane Proteins, Department of Physiology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Biol Chem 276:47886-94. 2001
    ..of AE1, the plasma membrane chloride/bicarbonate exchanger of erythrocytes, contains a binding site for carbonic anhydrase II (CAII)...
  4. pmc Activity and distribution of intracellular carbonic anhydrase II and their effects on the transport activity of anion exchanger AE1/SLC4A1
    Samer Al-Samir
    G Gros Zentrum Physiologie, Vegetative Physiologie 4220, Medizinische Hochschule Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany V Endeward Zentrum Physiologie 4220, Medizinische Hochschule Hannover, Germany
    J Physiol 591:4963-82. 2013
    ..metabolon hypothesis' postulating that a close association of the anion exchanger 1 (AE1) and cytosolic carbonic anhydrase II (CAII) exists that greatly increases the transport activity of AE1...
  5. ncbi A novel carbonic anhydrase II binding site regulates NHE1 activity
    Xiuju Li
    Department of Biochemistry, 347 Medical Science Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Biochemistry 45:2414-24. 2006
    b>Carbonic anhydrase II (CAII) binds to and regulates transport by the NHE1 isoform of the mammalian Na(+)/H(+) exchanger. We localized and characterized the CAII binding region on the C-terminal tail of the Na(+)/H(+) exchanger...
  6. ncbi Selective hydrophobic pocket binding observed within the carbonic anhydrase II active site accommodate different 4-substituted-ureido-benzenesulfonamides and correlate to inhibitor potency
    Fabio Pacchiano
    Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Box 100245, Gainesville, Florida 32610, USA
    Chem Commun (Camb) 46:8371-3. 2010
    ..Hydrophobic interactions between the scaffold of the inhibitors in different hydrophobic pockets of the enzyme were observed, explaining the diverse inhibitory range of these derivatives...
  7. ncbi Carbonic anhydrase II promotes cardiomyocyte hypertrophy
    Brittany F Brown
    Membrane Protein Disease Research Group, Department of Biochemistry, School of Translational Medicine, University of Alberta, Edmonton, AB, Canada
    Can J Physiol Pharmacol 90:1599-610. 2012
    ..b>Carbonic anhydrase II (CAII) provides substrates for these transporters (protons and bicarbonate, respectively)...
  8. pmc Overexpression of carbonic anhydrase II and Ki-67 proteins in prognosis of gastrointestinal stromal tumors
    Li Cheng Liu
    Department of General Surgery, General Hospital of PLA, Beijing 100853, China
    World J Gastroenterol 19:2473-80. 2013
    To investigate the expression and prognostic value of carbonic anhydrase II (CA II) and Ki-67 in gastrointestinal stromal tumors (GISTs).
  9. pmc Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus development suggest limited involvement in early CSF secretion
    Pia Johansson
    Department of Pharmacology, University of Melbourne, Parkville, Victoria 3010, Australia
    Neurosci Lett 442:77-80. 2008
    ..However, recent evidence suggests that the secretion may start much earlier, as soon as the lateral choroid plexuses first appear (around E14)...
  10. ncbi Timing and expression pattern of carbonic anhydrase II in pancreas
    Akari Inada
    Section of Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Dev Dyn 235:1571-7. 2006
    ..search for genetic markers for assessing the role of duct cells in pancreas growth, we examined whether carbonic anhydrase II (CAII) has ductal cell specificity...
  11. pmc Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange
    Danielle E Johnson
    Membrane Protein Research Group, Department of Physiology, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    J Physiol 589:1551-69. 2011
    ..The pH environment surrounding pH regulatory transport proteins may differ as a result of H+ microdomain formation, which will affect nearby pH-sensitive processes...
  12. ncbi An intramolecular transport metabolon: fusion of carbonic anhydrase II to the COOH terminus of the Cl(-)/HCO(3)(-)exchanger, AE1
    Daniel Sowah
    Membrane Protein Disease Research Group, Department of Physiology, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Cell Physiol 301:C336-46. 2011
    ..CAII. Taken together, these data suggest that association of functional CAII with AE1 increases Cl(-)/HCO(3)(-) exchange activity, consistent with the HCO(3)(-) transport metabolon model...
  13. ncbi The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II
    Deborah Sterling
    Canadian Institutes of Health Research Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Am J Physiol Cell Physiol 283:C1522-9. 2002
    COOH-terminal cytoplasmic tails of chloride/bicarbonate anion exchangers (AE) bind cytosolic carbonic anhydrase II (CAII) to form a bicarbonate transport metabolon, a membrane protein complex that accelerates transmembrane bicarbonate ..
  14. ncbi Directed evolution of the promiscuous esterase activity of carbonic anhydrase II
    Stephen McQ Gould
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Biochemistry 44:5444-52. 2005
    ..This work seeks to examine how this process might take place. Human carbonic anhydrase II (hCAII) is an enzyme that evolved to catalyze the reversible hydration of CO(2) and performs this task at ..
  15. pmc The paradoxical thermodynamic basis for the interaction of ethylene glycol, glycine, and sarcosine chains with bovine carbonic anhydrase II: an unexpected manifestation of enthalpy/entropy compensation
    Vijay M Krishnamurthy
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 128:5802-12. 2006
    This paper describes a systematic study of the thermodynamics of association of bovine carbonic anhydrase II (BCA) and para-substituted benzenesulfonamides with chains of oligoglycine, oligosarcosine, and oligoethylene glycol of lengths ..
  16. ncbi Sodium-hydrogen exchanger, cardiac overload, and myocardial hypertrophy
    Horacio E Cingolani
    Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, Calle 60 y 120, 1900 La Plata, Argentina
    Circulation 115:1090-100. 2007
  17. pmc Carbonic anhydrase II. A novel biomarker for gastrointestinal stromal tumors
    Seppo Parkkila
    Department of Anatomy, University of Tampere and Tampere University Hospital, Tampere, Finland
    Mod Pathol 23:743-50. 2010
    ..0001). The present results indicate that CA II is overexpressed in most GISTs, is quite selective to this tumor type among mesenchymal tumors, and therefore might be a useful biomarker in diagnostics...
  18. ncbi The importance of carbonic anhydrase II in red blood cells during exposure of chicken embryos to CO2
    N Everaert
    Department of Biosystems, Division Livestock Nutrition Quality, KU Leuven, Leuven, Belgium
    Respir Physiol Neurobiol 172:154-61. 2010
    ..This response may serve to "buffer" elevated CO(2) levels...
  19. ncbi Experimental immune-mediated pancreatitis in neonatally thymectomized mice immunized with carbonic anhydrase II and lactoferrin
    Kazushige Uchida
    Department of Gastroenterology and Endoscopic Medicine, Kyoto University Hospital, Kyoto, Japan
    Lab Invest 82:411-24. 2002
    We previously reported that autoantibodies against carbonic anhydrase II and lactoferrin are frequently identified in patients with autoimmune-related pancreatitis...
  20. pmc Carbonic anhydrase inhibition prevents and reverts cardiomyocyte hypertrophy
    Bernardo V Alvarez
    Department of Physiology, Membrane Protein Research Group, University of Alberta, Edmonton, Canada T6G2H7
    J Physiol 579:127-45. 2007
    ..Our study demonstrates a novel role for CA in mediating the hypertrophic response of cardiac myocytes to PE and suggests that CA inhibition represents an effective therapeutic approach towards mitigation of the hypertrophic phenotype...
  21. ncbi Carbonic anhydrase II increases the activity of the human electrogenic Na+/HCO3- cotransporter
    Holger M Becker
    Abteilung fur Allgemeine Zoologie, Fachbereich Biologie, Technische Universitat Kaiserslautern, P O Box 3049, D 67653 Kaiserslautern, Germany
    J Biol Chem 282:13508-21. 2007
    ..Our results show that the transport capacity of NBCe1 is enhanced by the catalytic activity of CAII, in line with the notion that CAII forms a transport metabolon with NBCe1...
  22. ncbi Phenotypic characteristics of bone in carbonic anhydrase II-deficient mice
    David S Margolis
    Orthopaedic Research Lab, Department of Orthopaedic Surgery, University of Arizona, Tucson, AZ 85721, USA
    Calcif Tissue Int 82:66-76. 2008
    b>Carbonic anhydrase II (CAII)-deficient mice were created to study the syndrome of CAII deficiency in humans including osteopetrosis, renal tubular acidosis, and cerebral calcification...
  23. ncbi Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor
    Daumantas Matulis
    Johnson and Johnson Pharmaceutical Research and Development, LLC, Eagleview Corporate Center, 665 Stockton Drive, Suite 104, Exton, Pennsylvania 19341, USA
    Biochemistry 44:5258-66. 2005
    ..The systematic effect of ligands on stability is shown to be a general tool to measure affinity...
  24. ncbi Effect of human carbonic anhydrase II on the activity of the human electrogenic Na/HCO3 cotransporter NBCe1-A in Xenopus oocytes
    Jing Lu
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:19241-50. 2006
    Others report that carbonic anhydrase II (CA II) binds to the C termini of the anion exchanger AE1 and the electrogenic Na/HCO3 cotransporter NBCe1-A, enhancing transport...
  25. pmc Protein adsorption orientation in the light of fluorescent probes: mapping of the interaction between site-directly labeled human carbonic anhydrase II and silica nanoparticles
    Martin Karlsson
    IFM Department of Chemistry, Linkoping University, Linkoping, Sweden
    Biophys J 88:3536-44. 2005
    ..nanoparticles, fluorescent probes were attached to different areas on the surface of the protein human carbonic anhydrase II. By this approach it was clearly demonstrated that the adsorption of the native protein is specific to ..
  26. ncbi Autoantibodies against carbonic anhydrase II are increased in renal tubular acidosis associated with Sjogren syndrome
    Fumi Takemoto
    Kidney Center, Toranomon Hospital, Tokyo, Japan
    Am J Med 118:181-4. 2005
  27. pmc Lactate flux in astrocytes is enhanced by a non-catalytic action of carbonic anhydrase II
    Malin H Stridh
    Division of General Zoology, Department of Biology, University of Kaiserslautern, PO Box 3049, D 67653 Kaiserslautern, Germany
    J Physiol 590:2333-51. 2012
    ..By this mechanism CAII could enhance transfer of lactate between astrocytes and neurons and thus provide the neurons with an increased supply of energy substrate...
  28. pmc Neutron diffraction of acetazolamide-bound human carbonic anhydrase II reveals atomic details of drug binding
    S Zoe Fisher
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    J Am Chem Soc 134:14726-9. 2012
    ..The structure reveals the molecular details of AZM binding and the charged state of the bound drug. This represents the first determined neutron structure of a clinically used drug bound to its target...
  29. ncbi QM/MM calculations reveal the different nature of the interaction of two carborane-based sulfamide inhibitors of human carbonic anhydrase II
    Adam Pecina
    Institute of Organic Chemistry and Biochemistry IOCB, Academy of Sciences of the Czech Republic, v v i, Gilead Sciences and IOCB Research Center, Flemingovo nam 2, 166 10, Prague 6, Czech Republic
    J Phys Chem B 117:16096-104. 2013
    The crystal structures of two novel carborane-sulfamide inhibitors in the complex with human carbonic anhydrase II (hCAII) have been studied using QM/MM calculations...
  30. ncbi Atomic crystal and molecular dynamics simulation structures of human carbonic anhydrase II: insights into the proton transfer mechanism
    S Zoe Fisher
    Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Biochemistry 46:2930-7. 2007
    Human carbonic anhydrase II (HCA II) is a zinc-metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3-...
  31. ncbi Inhibition profiling of human carbonic anhydrase II by high-throughput screening of structurally diverse, biologically active compounds
    Rema Iyer
    Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008 5410, USA
    J Biomol Screen 11:782-91. 2006
    Human carbonic anhydrase II (CA II), a zinc metalloenzyme, was screened against 960 structurally diverse, biologically active small molecules...
  32. ncbi Spectroscopic characterization of furosemide binding to human carbonic anhydrase II
    Samira Ranjbar
    Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
    Int J Biol Macromol 50:910-7. 2012
    This study reports the interaction between furosemide and human carbonic anhydrase II (hCA II) using fluorescence, UV-vis and circular dichroism (CD) spectroscopy...
  33. ncbi Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: a fluorescence spectroscopic analysis
    N A Bushmarina
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia
    Chembiochem 2:813-21. 2001
    GdmCl-, urea-, and pH-induced unfolding pathways of bovine carbonic anhydrase II have been analyzed by using changes induced by different denaturing agents in intensity, anisotropy, life time, and parameter A value of intrinsic ..
  34. pmc Purification of chicken carbonic anhydrase isozyme-III (CA-III) and its measurement in White Leghorn chickens
    Toshiho Nishita
    Laboratories of Veterinary Physiology, School of Veterinary Medicine, Azabu, University, Sagamihara, Kanagawa, Japan
    Acta Vet Scand 53:63. 2011
    ..The developmental profile of chicken carbonic anhydrase-III (CA-III) blood levels has not been previously determined or reported. We isolated CA-III from chicken muscle and investigated age-related changes in the levels of CA-III in blood...
  35. pmc Pretransition and progressive softening of bovine carbonic anhydrase II as probed by single molecule atomic force microscopy
    Rehana Afrin
    Laboratory of Biodynamics, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuka, Midori ku, Yokohama, 226 8501, Japan
    Protein Sci 14:1447-57. 2005
    ..force microscope (AFM) to extract information on the mechanical properties of surface immobilized bovine carbonic anhydrase II under native conditions and in the course of guanidinium chloride-induced denaturation...
  36. ncbi Anti-carbonic anhydrase II antibody in autoimmune pancreatitis and tubulointerstitial nephritis
    Hiroshi Nishi
    Nephrol Dial Transplant 22:1273-5. 2007
  37. pmc Crystal structure of human carbonic anhydrase II complexed with an anti-convulsant sugar sulphamate
    Rosario Recacha
    Center for Biophysical Sciences and Engineering, and School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochem J 361:437-41. 2002
    ..We have studied the binding interactions of RWJ-37497 in the active site of human carbonic anhydrase II by X-ray crystallography...
  38. ncbi Amyloid fibrillation in native and chemically-modified forms of carbonic anhydrase II: role of surface hydrophobicity
    Ali Es-haghi
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    Biochim Biophys Acta 1824:468-77. 2012
    ..In the present study, fibrillation potential of native and chemically-modified forms of bovine carbonic anhydrase II (BCA II) were investigated...
  39. ncbi Bicarbonate secretion by rat bile duct brush cells indicated by immunohistochemical localization of CFTR, anion exchanger AE2, Na+/HCO3 -cotransporter, carbonic anhydrase II, Na+/H+ exchangers NHE1 and NHE3, H+/K+-ATPase, and Na+/K+-ATPase
    Takuro Ogata
    Department of Surgery and Physiology, Kochi Medical School, Kohasu, Nankoku, Japan
    Med Mol Morphol 39:44-8. 2006
    ..Na(+)/HCO(3) (-) cotransporter (NBC), was found along the BLM but was absent on the MV, whereas carbonic anhydrase II (CA II) was observed on the MV and along the BLM...
  40. pmc Role of carbonic anhydrase II in ectopic calcification
    Rupak M Rajachar
    Bioengineering Department, University of Washington, Seattle, 98195, USA
    Cardiovasc Pathol 18:77-82. 2009
    ..Previous studies suggested that, in addition to blocking apatite crystal growth, OPN promoted regression of ectopic calcification by inducing the expression of acid-generating carbonic anhydrase II (CAR2) in monocyte-derived cells.
  41. ncbi Serum anti-carbonic anhydrase II antibodies and oxidant-antioxidant balance in pre-eclampsia
    Rezzan Aliyazicioglu
    Department of Biochemisty, Karadeniz Technical University, Trabzon, Turkey
    Am J Reprod Immunol 66:297-303. 2011
    PROBLEM  The aim of this study was to investigate the presence of anti-carbonic anhydrase II antibodies (anti-CA II) antibodies in pre-eclampsia and the relationships between the autoantibodies, total antioxidant capacity (TAC) and ..
  42. ncbi Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II
    Zenta Yasukawa
    Laboratory of Animal Cell Function, Bioscience and Biotechnology Center, Nagoya University, Nagoya 464 8601, Japan
    J Biochem 141:429-41. 2007
    ..The 32-kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of ..
  43. ncbi The expression of carbonic anhydrase II in hematological malignancies
    Mari Leppilampi
    Departments of Anatomy and Cell Biology, Clinical Chemistry, University of Oulu, 90220 Oulu, Finland
    Clin Cancer Res 8:2240-5. 2002
    ..Recent studies have shown that they are functionally involved in the growth and invasion of cancer cells. However, there are only a few publications on CAs in hematological malignancies...
  44. ncbi Carbonic anhydrase II autoantibody and oxidative stress in rheumatoid arthritis
    Ahmet Alver
    Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
    Clin Biochem 44:1385-9. 2011
    ..To investigate the relationship between carbonic anhydrase (CA) II autoantibody and lipid peroxidation, certain antioxidant parameters, and cytokines in rheumatoid arthritis (RA) patients...
  45. pmc Fluoroalkyl and alkyl chains have similar hydrophobicities in binding to the "hydrophobic wall" of carbonic anhydrase
    Jasmin Mecinovic
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States
    J Am Chem Soc 133:14017-26. 2011
    ..alkyl and fluoroalkyl chains (H(2)NSO(2)C(6)H(4)-CONHCH(2)(CX(2))(n)CX(3), n = 0-4, X = H, F) to human carbonic anhydrase II (HCA II)...
  46. ncbi Dramatic stabilization of the native state of human carbonic anhydrase II by an engineered disulfide bond
    Lars Göran Mårtensson
    IFM Department of Chemistry, Linkoping University, SE 581 83 Linkoping, Sweden
    Biochemistry 41:15867-75. 2002
    To find a disulfide pair that could stabilize the enzyme human carbonic anhydrase II (HCA II), we grafted the disulfide bridge from the related and unusually stable carbonic anhydrase form from Neisseria gonorrhoeae (NGCA) into the human ..
  47. ncbi Potential for differential diagnosis of autoimmune pancreatitis and pancreatic cancer using carbonic anhydrase II antibody
    Hideo Hosoda
    Department of Clinical Laboratory Medicine, Tokyo Kosei Nenkin Hospital, Tokyo, Japan
    Pancreas 37:e1-7. 2008
    ..We previously found a small amount of an immunoglobulin G-like material in purchased CAII reagents, which led to pseudopositive reactions...
  48. ncbi Carbonic anhydrase II regulates differentiation of ameloblasts via intracellular pH-dependent JNK signaling pathway
    Xiaogu Wang
    Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan
    J Cell Physiol 225:709-19. 2010
    ..We found that the expression level of carbonic anhydrase II (CAII) is strongly up-regulated in parallel with differentiation of enamel epithelium tissues, while the ..
  49. pmc Role of hydrophilic residues in proton transfer during catalysis by human carbonic anhydrase II
    Jiayin Zheng
    Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Biochemistry 47:12028-36. 2008
    Catalysis by the zinc metalloenzyme human carbonic anhydrase II (HCA II) is limited in maximal velocity by proton transfer between His64 and the zinc-bound solvent molecule...
  50. pmc Neutron structure of human carbonic anhydrase II: implications for proton transfer
    S Zoe Fisher
    Bioscience Division MS M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87544, USA
    Biochemistry 49:415-21. 2010
    Human carbonic anhydrase II (HCA II) catalyzes the reversible hydration of carbon dioxide to form bicarbonate and a proton...
  51. ncbi Fragment-based drug discovery of carbonic anhydrase II inhibitors by dynamic combinatorial chemistry utilizing alkene cross metathesis
    Sally Ann Poulsen
    Chemical Biology Group, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan Campus, Brisbane 4111, Australia
    Bioorg Med Chem 14:3275-84. 2006
    ..drug discovery approach to the synthesis and identification of small molecule inhibitors of bovine carbonic anhydrase II (bCA II) is described...
  52. ncbi Inhibition of carbonic anhydrase II by steroidal and non-steroidal sulphamates
    Y T Ho
    Endocrinology and Metabolic Medicine and Sterix Ltd, Faculty of Medicine, Imperial College, St Mary s Hospital, London W2 1NY, UK
    Biochem Biophys Res Commun 305:909-14. 2003
    ....
  53. ncbi Nonenzymatic augmentation of lactate transport via monocarboxylate transporter isoform 4 by carbonic anhydrase II
    Holger M Becker
    Arbeitsgruppe Zoologie Membrantransport, FB Biologie, TU Kaiserslautern, P O Box 3049, 67653, Kaiserslautern, Germany
    J Membr Biol 234:125-35. 2010
    ..1 (MCT1), heterologously expressed in Xenopus oocytes, has previously been shown to be supported by carbonic anhydrase II (CAII) in a noncatalytic manner...
  54. pmc Intramolecular proton shuttle supports not only catalytic but also noncatalytic function of carbonic anhydrase II
    Holger M Becker
    AG Zoologie Membrantransport and Abteilung für Allgemeine Zoologie, FB Biologie, University of Kaiserslautern, D 67653 Kaiserslautern, Germany
    Proc Natl Acad Sci U S A 108:3071-6. 2011
    ..In carbonic anhydrase II (CAII), the fastest and most wide-spread isoform, this H(+) shuttle is facilitated by the side chain of ..
  55. ncbi Designed, functionalized helix-loop-helix motifs that bind human carbonic anhydrase II: a new class of synthetic receptor molecules
    Karin Enander
    Department of Chemistry IFM, Linkoping University, 581 83 Linkoping, Sweden
    J Am Chem Soc 126:4464-5. 2004
    ..four-helix bundles were functionalized by the introduction of a sulfonamide derivative known to bind human carbonic anhydrase II (HCAII) and one or both of the dansyl- and methoxycoumarin fluorescent probes...
  56. pmc Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
    Toshiho Nishita
    Laboratories of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, 1 17 71 Fuchinobe, Sagamihara, Kanagawa, 252 5201, Japan
    Acta Vet Scand 53:16. 2011
    ..Furthermore, the relations with CA-II in erythrocyte and egg production are not discussed. In the present study, we isolated CA-II from erythrocytes of chickens and determined age-related changes of CA-II levels in erythrocytes...
  57. ncbi Investigations of the esterase, phosphatase, and sulfatase activities of the cytosolic mammalian carbonic anhydrase isoforms I, II, and XIII with 4-nitrophenyl esters as substrates
    Alessio Innocenti
    Universita degli Studi di Firenze, Laboratorio di Chimica Bioinorganica, Rm 188, Via della Lastruccia 3, I 50019 Sesto Fiorentino, Firenze, Italy
    Bioorg Med Chem Lett 18:2267-71. 2008
    ..CA XIII was the most effective esterase and phosphatase. CA XIII might catalyze other physiological reactions than CO(2) hydration, based on its relevant phosphatase activity...
  58. ncbi Carbonic anhydrase inhibitors: the X-ray crystal structure of ethoxzolamide complexed to human isoform II reveals the importance of thr200 and gln92 for obtaining tight-binding inhibitors
    Anna Di Fiore
    Istituto di Biostrutture e Bioimmagini CNR, Via Mezzocannone 16, 80134 Napoli, Italy
    Bioorg Med Chem Lett 18:2669-74. 2008
    ....
  59. ncbi Determination of the thermodynamics of carbonic anhydrase acid-unfolding by titration calorimetry
    Lina Baranauskiene
    Institute of Biotechnology, Graiciuno 8, Vilnius, LT 02241, Lithuania
    J Biochem Biophys Methods 70:1043-7. 2008
    The enthalpy of unfolding (DeltauH) of carbonic anhydrase II was determined by titrating the protein with acid and measuring the heat using isothermal titration calorimetry (ITC) in the temperature range of 5 to 59 degrees C...
  60. ncbi Carbonic anhydrase II deficiency syndrome (osteopetrosis with renal tubular acidosis and brain calcification): novel mutations in CA2 identified by direct sequencing expand the opportunity for genotype-phenotype correlation
    Gul N Shah
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, MO 63104, USA
    Hum Mutat 24:272. 2004
    The carbonic anhydrase II (CA II) deficiency syndrome is an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis, and cerebral calcification...
  61. ncbi High prevalence of autoantibodies against carbonic anhydrase II and lactoferrin in type 1 diabetes: concept of autoimmune exocrinopathy and endocrinopathy of the pancreas
    Takao Taniguchi
    Department of Internal Medicine, Ohtsu Red Cross Hospital, Shiga, Japan
    Pancreas 27:26-30. 2003
    ..Dysfunction of the exocrine as well as the endocrine pancreas has been reported in type 1 diabetes. Lymphocytic infiltration of the exocrine pancreas is observed in approximately half of Japanese type 1 diabetic patients...
  62. ncbi Unfolding a folding disease: folding, misfolding and aggregation of the marble brain syndrome-associated mutant H107Y of human carbonic anhydrase II
    Karin Almstedt
    IFM Department of Chemistry, Linkoping University, 581 83 Linkoping, Sweden
    J Mol Biol 342:619-33. 2004
    ..These proteins often misfold due to mutations. The disease marble brain syndrome (MBS), known also as carbonic anhydrase II deficiency syndrome (CADS), can manifest in carriers of point mutations in the human carbonic anhydrase II ..
  63. ncbi Docking, synthesis, and in vitro evaluation of antimitotic estrone analogs
    Andre Stander
    Department of Physiology, University of Pretoria, Pretoria, South Africa
    Chem Biol Drug Des 77:173-81. 2011
    ....
  64. ncbi Benzimidazo[1,2-c][1,2,3]thiadiazole-7-sulfonamides as inhibitors of carbonic anhydrase
    Virginija Dudutiene
    Laboratory of Biothermodynamics and Drug Design, Institute of Biotechnology, Vilnius LT 02241, Lithuania
    Bioorg Med Chem Lett 17:3335-8. 2007
    ..Human carbonic anhydrase I (hCAI) and bovine carbonic anhydrase II (bCAII) bound the inhibitors with observed association constants in the range from 1.1 x 10(6) to 2...
  65. pmc Carbonic anhydrase isozyme-II-deficient mice lack the duodenal bicarbonate secretory response to prostaglandin E2
    Mari Leppilampi
    Institute of Medical Technology, University of Tampere and Tampere University Hospital, FI 33520 Tampere, Finland
    Proc Natl Acad Sci U S A 102:15247-52. 2005
    ..Our data demonstrate a gastrointestinal phenotypic abnormality associated with CA II deficiency. The results show that the stimulatory effect of the duodenal secretagogue PGE2 completely depends on CA II...
  66. ncbi Immunohistochemical co-expression of carbonic anhydrase II with Kv1.4 and TRPV1 in rat small-diameter trigeminal ganglion neurons
    Takeshi Tanimoto
    Department of Physiology, Nippon Dental University, School of Dentistry at Tokyo, 1 9 20 Fujimi, Tokyo 102 8159, Japan
    Brain Res 1044:262-5. 2005
    The co-expression of carbonic anhydrase II (CAII) with the voltage-gated potassium channel subtype 1.4 (Kv1...
  67. pmc Regulation of the sodium bicarbonate cotransporter kNBC1 function: role of Asp(986), Asp(988) and kNBC1-carbonic anhydrase II binding
    Eitan Gross
    Department of Urology, Case Western Reserve University, Cleveland, OH, USA
    J Physiol 544:679-85. 2002
    ..A homologous motif (D887ADD) in the carboxy-terminus of the anion exchanger AE1 binds to carbonic anhydrase II (CAII)...
  68. ncbi Substrate-dependent interference of carbonic anhydrases with the glutamine transporter SNAT3-induced conductance
    Alexandra Weise
    Abteilung fur Allgemeine Zoologie, FB Biologie, University of Kaiserslautern, Kaiserslautern, Germany
    Cell Physiol Biochem 27:79-90. 2011
    ..protons, and displays a non-stoichiometrical conductance, which is suppressed by the catalytic activity of carbonic anhydrase II (CAII)...
  69. ncbi Reshaping the folding energy landscape of human carbonic anhydrase II by a single point genetic mutation Pro237His
    Yan Jiang
    Sichuan University, Chengdu 610064, Sichuan, People s Republic of China
    Int J Biochem Cell Biol 40:776-88. 2008
    ..The results herein suggested that the correct positioning of the long loop around P237 might be crucial to the folding of HCA II, particularly the formation of the active site...
  70. pmc Enzymatic suppression of the membrane conductance associated with the glutamine transporter SNAT3 expressed in Xenopus oocytes by carbonic anhydrase II
    Alexandra Weise
    Abteilung fur Allgemeine Zoologie, FB Biologie, Universitaet Kaiserslautern, D 67653 Kaiserslautern, Germany
    J Gen Physiol 130:203-15. 2007
    ..This study also emphasizes the importance of carbonic anhydrase activity and the presence of CO(2)-bicarbonate buffers for membrane transport processes...
  71. ncbi The cyclooxygenase-2 inhibitor celecoxib is a potent inhibitor of human carbonic anhydrase II
    James F Knudsen
    Division of Clinical Pharmacology and Medical Toxicology, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    Inflammation 28:285-90. 2004
    ..we report that celecoxib exhibits the characteristics of a potent CA inhibitor, showing inhibitory human carbonic anhydrase II (hCAII) activity in the nanomolar range. Valdecoxib was relatively less potent...
  72. ncbi The binding of human carbonic anhydrase II by functionalized folded polypeptide receptors
    Theresa Andersson
    Department of Chemistry IFM, Linkoping University, 581 83 Linkoping, Sweden
    Chem Biol 12:1245-52. 2005
    Several receptors for human carbonic anhydrase II (HCAII) have been prepared by covalently attaching benzenesulfonamide carboxylates via aliphatic aminocarboxylic acid spacers of variable length to the side chain of a lysine residue in a ..
  73. pmc Elucidation of the proton transport mechanism in human carbonic anhydrase II
    C Mark Maupin
    Center for Biophysical Modeling and Simulation and Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    J Am Chem Soc 131:7598-608. 2009
    Human carbonic anhydrase II (HCA II) is one of the fastest known enzymes, which utilizes a rate-limiting proton transport (PT) step in its enzymatic reaction...
  74. ncbi Deficient HCO3- transport in an AE1 mutant with normal Cl- transport can be rescued by carbonic anhydrase II presented on an adjacent AE1 protomer
    Neera K Dahl
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:44949-58. 2003
    Cl-/HCO3- exchange activity mediated by the AE1 anion exchanger is reduced by carbonic anhydrase II (CA2) inhibition or by prevention of CA2 binding to the AE1 C-terminal cytoplasmic tail...
  75. ncbi Diminished cellular immune response to carbonic anhydrase II in patients with Sjögren's syndrome and idiopathic chronic pancreatitis
    Isao Nishimori
    Department of Gastroenterology and Hepatology, Kochi Medical School Nankoku, Kochi, Japan
    JOP 5:186-92. 2004
    A serum antibody to carbonic anhydrase II has been reported in patients with Sjögren's syndrome and idiopathic chronic pancreatitis.
  76. ncbi Suppression of adjuvant-induced arthritic bone destruction by cyclooxygenase-2 selective agents with and without inhibitory potency against carbonic anhydrase II
    Mika Katagiri
    Sensory and Motor System Medicine, Faculty of Medicine, University of Tokyo, Bunkyo, Tokyo, Japan
    J Bone Miner Res 21:219-27. 2006
    ..However, all COX-2 inhibitors similarly suppressed bone destruction in adjuvant-induced arthritic rats, indicating that suppression of osteoclast differentiation is more effective than that of osteoclast activity for the treatment...
  77. ncbi Functional role of a putative carbonic anhydrase II-binding domain in the electrogenic Na+ -HCO₃- cotransporter NBCe1 expressed in Xenopus oocytes
    Hideomi Yamada
    Department of Internal Medicine, The University of Tokyo, Tokyo, Japan
    Channels (Austin) 5:106-9. 2011
    ..These results indicate that a bicarbonate transport metabolon proposed for the interaction between CAII and NBCe1 does not work at least in Xenopus oocytes...
  78. pmc Modification of carbonic anhydrase II with acetaldehyde, the first metabolite of ethanol, leads to decreased enzyme activity
    Fatemeh Bootorabi
    Institute of Medical Technology, Tampere University Hospital, 33520 Tampere, Finland
    BMC Biochem 9:32. 2008
    ....
  79. ncbi Measurement of erythrocyte carbonic anhydrase isozymes (CA-I and CA-II) in racehorses and riding horses
    Toshiho Nishita
    Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa, Japan
    J Vet Med Sci 67:63-7. 2005
    ..The CA-II level decreased slightly with age, indicating different regulation of CA-I and CA-II expression during development...
  80. ncbi Differential expression of cytoplasmic carbonic anhydrases, CA I and II, and membrane-associated isozymes, CA IX and XII, in normal mucosa of large intestine and in colorectal tumors
    A J Kivela
    Department of Anatomy and Cell Biology, University of Oulu, Finland
    Dig Dis Sci 46:2179-86. 2001
    ....
  81. ncbi Nonenzymatic proton handling by carbonic anhydrase II during H+-lactate cotransport via monocarboxylate transporter 1
    Holger M Becker
    Abteilung fur Allgemeine Zoologie, FB Biologie, Technische Universitat Kaiserslautern, Kaiserslautern, Germany
    J Biol Chem 283:21655-67. 2008
    ..We present a hypothetical model in which the effective movement of H(+) into the bulk cytosol is increased by CAII, thus slowing the dissipation of the H(+) gradient across the cell membrane, which drives MCT1 activity...
  82. ncbi Speeding up proton transfer in a fast enzyme: kinetic and crystallographic studies on the effect of hydrophobic amino acid substitutions in the active site of human carbonic anhydrase II
    S Zoe Fisher
    Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida 32610 0267, USA
    Biochemistry 46:3803-13. 2007
    ..Finally, the difference in pKa of the donor and acceptor appears favorable for proton transfer. The data suggest roles for residues 7, 62, and 67 in fine-tuning the properties of His64 for optimal proton transfer in catalysis...
  83. pmc A short, strong hydrogen bond in the active site of human carbonic anhydrase II
    Balendu Sankara Avvaru
    Department of Biochemistry and Molecular Biology, Universityof Florida, Gainesville, Florida 32610, USA
    Biochemistry 49:249-51. 2010
    The crystal structure of human carbonic anhydrase II (HCA II) obtained at 0...
  84. ncbi QSAR study on para-substituted aromatic sulfonamides as carbonic anhydrase II inhibitors using topological information indices
    Georgia Melagraki
    Laboratory of Organic Chemistry, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Greece
    Bioorg Med Chem 14:1108-14. 2006
    ..The compounds were studied for their carbonic anhydrase II (CAII) inhibitory activity...
  85. ncbi Carbonic anhydrase II is a tumor vessel endothelium-associated antigen targeted by dendritic cell therapy
    Kenta Yoshiura
    Department of Advanced Medical Science, University of Tokyo, Japan
    Clin Cancer Res 11:8201-7. 2005
    ..by two-dimensional electrophoresis combined with Western blots revealed that the 29-kDa protein was carbonic anhydrase II (CA-II)...
  86. pmc Structure of a 129Xe-cryptophane biosensor complexed with human carbonic anhydrase II
    Julie A Aaron
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    J Am Chem Soc 130:6942-3. 2008
    ..70 A resolution crystal structure of a cryptophane-derivatized benezenesulfonamide complexed with human carbonic anhydrase II shows how an encapsulated xenon atom can be directed to a specific biological target...
  87. ncbi Subtle differences in dissociation rates of interactions between destabilized human carbonic anhydrase II mutants and immobilized benzenesulfonamide inhibitors probed by a surface plasmon resonance biosensor
    S Svedhem
    Division of Chemistry, , , S-581 83, Sweden
    Anal Biochem 296:188-96. 2001
    ..Here, a set of destabilized human carbonic anhydrase II (HCA II) mutants was investigated with respect to their interaction kinetics with two different ..
  88. ncbi In situ click chemistry: enzyme-generated inhibitors of carbonic anhydrase II
    Vani P Mocharla
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, SP 227, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 44:116-20. 2004
  89. ncbi Carbonic anhydrase II in the developing and adult human brain
    Elizabeth Kida
    Department of Developmental Neurobiology, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314, USA
    J Neuropathol Exp Neurol 65:664-74. 2006
    b>Carbonic anhydrase II (CA II) is one of 14 isozymes of carbonic anhydrases, zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate...
  90. ncbi Residual ligand entropy in the binding of p-substituted benzenesulfonamide ligands to bovine carbonic anhydrase II
    Henning Stöckmann
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    J Am Chem Soc 130:12420-6. 2008
    ..for this assumption, and in the case of binding of p-substituted benzenesulfonamide inhibitors to bovine carbonic anhydrase II (BCA II), the observed thermodynamic binding signature derived from isothermal titration calorimetry ..
  91. ncbi Phase memory relaxation times of spin labels in human carbonic anhydrase II: pulsed EPR to determine spin label location
    M Huber
    Department of Molecular Physics, Leiden University, P O Box 9504, 2300 RA Leiden, The Netherlands
    Biophys Chem 94:245-56. 2001
    Phase memory relaxation times (T(M) or T(2)) of spin labels in human carbonic anhydrase II (HCA II) are reported...
  92. ncbi Co-expression of pendrin, vacuolar H+-ATPase alpha4-subunit and carbonic anhydrase II in epithelial cells of the murine endolymphatic sac
    Hongwei Dou
    Center for Hearing and Deafness Research, Department of Pediatric Otolaryngology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    J Histochem Cytochem 52:1377-84. 2004
    ....
  93. pmc In vivo repression of an erythroid-specific gene by distinct corepressor complexes
    Luc E G Rietveld
    Department of Molecular Biology, NCMLS 191, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    EMBO J 21:1389-97. 2002
    To assess the mechanisms of repression of the erythroid-specific carbonic anhydrase II (CAII) locus we used chromatin immunoprecipitation and show that an NCoR-histone deacetylase (HDAC)3 complex is recruited by the nuclear receptor v-..
  94. pmc Carbonic anhydrase II and alveolar fluid reabsorption during hypercapnia
    Jiwang Chen
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    Am J Respir Cell Mol Biol 38:32-7. 2008
    b>Carbonic anhydrase II (CAII) plays an important role in carbon dioxide metabolism and intracellular pH regulation...
  95. ncbi Application of genetic algorithm-kernel partial least square as a novel nonlinear feature selection method: activity of carbonic anhydrase II inhibitors
    Mehdi Jalali-Heravi
    Department of Chemistry, Sharif University of Technology, Tehran, Iran
    Eur J Med Chem 42:649-59. 2007
    ..a nonlinear QSAR model for predicting activities of a series of substituted aromatic sulfonamides as carbonic anhydrase II (CA II) inhibitors...
  96. ncbi Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA
    Frederick B Loiselle
    Canadian Institute of Health Research Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Canada T6G 2H7
    Am J Physiol Cell Physiol 286:C1423-33. 2004
    ..Cytosolic enzyme carbonic anhydrase II (CAII) catalyzes the reaction CO(2) + H(2)O left arrow over right arrow HCO(3)(-) + H(+) in many tissues...
  97. ncbi Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells
    Kyosuke Fujisaki
    Nihon University Graduate School of Dentistry, 1 8 13 Kanda Surugadai, Chiyoda ku, Tokyo 101 8310, Japan
    Life Sci 80:1311-8. 2007
    ..examined the effects of IL-1alpha or RANKL and/or M-CSF in the presence of IL-1alpha on the expression of carbonic anhydrase II (CAII), cathepsin K, matrix metalloproteinase-9 (MMP-9), RANK, M-CSF receptor (c-fms), and c-fos ..
  98. ncbi Increased expression of carbonic anhydrase II (CA II) in lesional skin of atopic dermatitis: regulation by Th2 cytokines
    Marijke Kamsteeg
    J Invest Dermatol 127:1786-9. 2007
  99. ncbi The refined atomic structure of carbonic anhydrase II at 1.05 A resolution: implications of chemical rescue of proton transfer
    David Duda
    Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA
    Acta Crystallogr D Biol Crystallogr 59:93-104. 2003
    ..radiation and a CCD detector, X-ray data have been collected at 100 K for the His64Ala mutant of human carbonic anhydrase II complexed with 4-methylimidazole (4-MI) to a maximal 1...
  100. ncbi The importance of being knotted: effects of the C-terminal knot structure on enzymatic and mechanical properties of bovine carbonic anhydrase II
    Mohammad Taufiq Alam
    Laboratory of Biodynamics, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, Japan
    FEBS Lett 519:35-40. 2002
    ..pattern to the enzymatic and mechanical properties of carbonic anhydrases, we replaced Gln-253 of bovine carbonic anhydrase II with Cys, which allowed us to measure the mechanical strength of the protein against tensile deformation ..
  101. ncbi Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties
    Anna Di Fiore
    Istituto di Biostrutture e Bioimmagini CNR, Via Mezzocannone 16, 80134 Naples, Italy
    Bioorg Med Chem Lett 17:1726-31. 2007
    ..Topiramate, another sugar-based CA inhibitor, binds in a completely different manner to CA II as compared to the sulfonamide investigated here. These findings are useful for the design of potent, sugar-derived enzyme inhibitors...

Research Grants39

  1. H+-ATPase B-subunit Dysfunction and Calcium Nephrolithiasis
    Orson W Moe; Fiscal Year: 2010
    ..caused by mutations in either the vacuolar :VPa;se-A T :B1 or a4 subunit;, anion exchanger-1, or carbonic anhydrase II. This proposal focuses on the B1 subunit of the V-ATPase...
  2. Physiology of Electrogenic Na/HCO3 Cotransporters
    Walter F Boron; Fiscal Year: 2011
    ..on Na+, HCO3 and pH;ask whether they transport CO3;characterize the interaction between NBCel and carbonic anhydrase II;and examine the action of PKA on NBCel in oocytes. (Hi) Analyze structure- function relationships...
  3. CELLULAR BASIS OF CRANIOFACIAL BONE DISORDERS
    Philip Osdoby; Fiscal Year: 2002
    ..effects on OC function (bone resorption, motility, free radical production, TRAP, cathepsin K, and carbonic anhydrase II expression)...
  4. Supramolecular Approaches to Protein Inhibitors Activated by MicroRNA-21
    Janarthanan Jayawickramarajah; Fiscal Year: 2013
    ....
  5. REGULATION OF CARBONIC ANHYDRASE II GENE EXPRESSION
    LUCYNDIA MARINO; Fiscal Year: 1993
    ..The OH- that is generated by this pump is converted to HCO3- by the action of a second enzyme, carbonic anhydrase II (CA II)...
  6. MUTAGENESIS TO PROBE CATALYSIS BY CARBONIC ANHYDRASE II
    Carol Fierke; Fiscal Year: 1992
    This proposal outlines a research effort to delineate the roles of active site amino acids in human carbonic anhydrase II (CA II) in catalysis and drug (sulfonamide) binding...
  7. INTERACTION OF SPIN LABELS WITH TRANSITION METALS
    GARETH EATON; Fiscal Year: 2002
    ..We will test the feasibility of measurements in fluid solution. For doubly spin-labeled samples of human carbonic anhydrase II, T4 lysozyme, and iron protein A we propose to develop and test pulsed methods to determine spin-spin ..
  8. FOLDING OF CARBONIC ANHYDRASE
    Sandra Eaton; Fiscal Year: 1999
    ..folding intermediate and unfolded state that are observed when guanidine hydrochloride is added to human carbonic anhydrase II. It is anticipated that these techniques will be useful in studies of other proteins to which the spin ..
  9. CIRCULAR DICHROISM SPECTROMETER
    TERRENCE OAS; Fiscal Year: 1993
    ..Designed proteins 2. Protein subdomains 3. Model protein folding intermediates 4. RNA enzymes 5. Human carbonic anhydrase II 6. Glycoproteins 7. Lectins 8. Membrane-associated proteins 9. Nucleic acids 10...
  10. Recombinant T-cell Receptor Ligands for Treatment of Uveitis
    Grazyna Adamus; Fiscal Year: 2006
    ..To determine an effective treatment regimen for clinical rat AAU using RTLs. 2. To determine the abilities of RTLs to inhibit recurrent anterior and posterior uveitis in Lewis rats. [unreadable] [unreadable] [unreadable] [unreadable]..
  11. MECHANISM OF RETINAL DAMAGE IN AUTOIMMUNE RETINOPATHY
    Grazyna Adamus; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  12. ION CHANNEL REGULATION BY THE CYTOPLASMIC TAIL OF PDK1
    Seth Alper; Fiscal Year: 2005
    ..We will search for novel interacting proteins, and prepare protein suitable for structural analysis. ..
  13. RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
    Seth Alper; Fiscal Year: 2009
    ..The proposed experiments will increase understanding of sickle cell disease and thalassemia by providing mouse models for genetic tests of new drug therapies under development for near-term clinical testing. ..
  14. Sickle Red Cell K+ Transporter Genetics in S. cerevisiae
    Seth Alper; Fiscal Year: 2002
    ..3) (Provisional Aim): Time permitting, to improve, validate, and standardize growth rescue of trk1delta/trk2delta S. cerevisiae by expression in nonpermissive conditions of cDNA encoding the mammalian IK1 K(ca) channel. ..
  15. MOLECULAR PHYSIOLOGY OF BAND 3 LIKE PROTEINS OF KIDNEY
    Seth Alper; Fiscal Year: 2007
    ..5. Define aspects of transcriptional and translational regulation of AE gene products in kidney of mutant and parental mouse strains and in cultured kidney cells. ..
  16. IMMUNOLOGICAL MECHANISM OF INFLAMMATION IN UVEITIS & EAE
    Grazyna Adamus; Fiscal Year: 2001
    ..3) Examine the role of cytokines and chemokines in the recruitment of cell to the eye in primary and recurrent uveitis associated with EAE after immunization of Lewis rats with MBP. ..
  17. Strategies to Identify In Vitro Islet Progenitors
    Susan Bonner Weir; Fiscal Year: 2007
    ..Identifying such cell(s) would then direct focus on the expansion and differentiation of that cell type to a sustainable source of new beta cells. [unreadable] [unreadable]..
  18. Solubilization, purification, and crystallization of memebrane-associated protein
    David G Myszka; Fiscal Year: 2010
    ..Streamlining crystallography efforts while simultaneously discovering details about receptor function will serve as the basis for developing agents that thwart, control, or cure GPCR-related diseases and conditions. ..
  19. Identification of GCK-3 signaling pathways in C. elegans
    KEITH CHOE; Fiscal Year: 2008
    ..I will use a combination of molecular biology, forward and reverse genetic analyses, and patch clamp electrophysiology to identify GCK-3 signaling components. ..
  20. Phenotypic Analysis of Young and Old Beta Cells
    Susan Bonner Weir; Fiscal Year: 2008
    ..Another important outcome of this research should be valuable information about such practical questions as what will lead to successful long-term islet transplantation. ..
  21. Chemosensory receptors and the basis of specificity
    STEVEN MUNGER; Fiscal Year: 2009
    ..abstract_text> ..
  22. Multivalency: Mechanisms and Applications
    George M Whitesides; Fiscal Year: 2010
    ....
  23. ASM Conference on Candida and Candidiasis
    Aaron Mitchell; Fiscal Year: 2008
    ..The conference will help chart the course of future research and facilitate the urgent necessity to understand, treat, and prevent disease. [unreadable] [unreadable] [unreadable]..
  24. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2009
    ..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..
  25. Capillary Electrophoresis and Protein Biophysics
    George M Whitesides; Fiscal Year: 2010
    ....
  26. ProteOn XPR36 Protein Interaction Array System
    David Myszka; Fiscal Year: 2007
    ..Each of these investigators has previously demonstrated success in applying biosensor technology in their research and all have a current need for the enhanced capabilities of this new platform. [unreadable] [unreadable]..
  27. SEXUAL DIMORPHISM IN SEIZURE CONTROL
    JANA VELISKOVA; Fiscal Year: 2002
    ..Rats of both sexes and various ages will be used. These studies may lead to the development of new antiepileptic treatments that take into the account sexual age-related differences. ..
  28. CELL GROWTH, NA/H EXCHANGE, AND CYCLINS IN IDDM PATIEN
    Daniel Batlle; Fiscal Year: 2002
    ....
  29. PHYSIOLOGY OF RENAL SODIUM/BICARBONATE COTRANSPORT
    Walter Boron; Fiscal Year: 2001
    ..The use of isolated, perfused tubules will insure that the expression of the transporters is as normal as possible, and that anatomical origin of the tubules is precisely known. ..
  30. Fourier Transform Ion Cyclotron Resonance Mass Spect.
    Mehdi Moini; Fiscal Year: 2003
    ..3) Chemical Composition Analysis of Expanded Porphyrins. Some of these expanded porphyrins are used to develop drugs for diagnosis and treatment of human diseases such as radiation therapy enhancing agents. ..
  31. CHARACTERIZING SPECIALIZED CHEMOSENSORY NEURONS
    STEVEN MUNGER; Fiscal Year: 2002
    ..In addition, GC-D transgenic mice will be used to examine the ability of neonates to attach to nipples and suckle. ..
  32. CHIMERIC RNA/DNA OLIGONUCLEOTIDE BASED GENE THERAPY
    YEONG HAU LIEN; Fiscal Year: 2003
    ..encouraging results, the investigators propose to test this new strategy in an experimental animal model, carbonic anhydrase II (CA-II) deficient mice...
  33. New strategies for identification of C. albicans fluconazole resistance gene
    Aaron Mitchell; Fiscal Year: 2004
    ..These approaches will define the regulators that govern known resistance mechanisms and may reveal the regulators that govern other clinically relevant resistance mechanisms. ..
  34. Prenatal Corticosteroid Impact on Hippocampus
    LIBOR VELISEK; Fiscal Year: 2004
    ..The data of this proposal will be critical for determining long-term adverse effects of multiple course prenatal corticosteroid therapy and for the ameliorating these effects in the offspring. ..
  35. Tools To Study Cell Motility and Ion Channels
    George Whitesides; Fiscal Year: 2006
    ..The work spans biology, microfabrication, and biophysics, and an important product of the research will be students broadly trained in biological and physical sciences. ..
  36. Biacore S51 Optical Biosensor
    David Myszka; Fiscal Year: 2002
    ..When time permits, the BIAcore S51 will be made available for use by external researchers as part of the Facility's effort to establish a national center for biomolecular interaction analysis. ..
  37. Structural Aspects of Bicarbonate Transport Metabolons
    Peter Piermarini; Fiscal Year: 2005
    ..The proposed study will provide key details on the finer mechanisms of renal HCO3 transport, which will provide a better understanding of the physiology and importance of the studied proteins to kidney function. ..