5 methyltetrahydrofolate homocysteine s methyltransferase

Summary

Summary: An enzyme that catalyzes the formation of methionine by transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine. It requires a cobamide coenzyme. The enzyme can act on mono- or triglutamate derivatives. EC 2.1.1.13.

Top Publications

  1. ncbi Methionine synthase D919G polymorphism, folate metabolism, and colorectal adenoma risk
    Ellen L Goode
    Cancer Prevention Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cancer Epidemiol Biomarkers Prev 13:157-62. 2004
  2. ncbi Influence of a methionine synthase (D919G) polymorphism on plasma homocysteine and folate levels and relation to risk of myocardial infarction
    J Chen
    Department of Epidemiology, Harvard School of Public Health, Boston, MA, USA
    Atherosclerosis 154:667-72. 2001
  3. ncbi Polygenic influence on plasma homocysteine: association of two prevalent mutations, the 844ins68 of cystathionine beta-synthase and A(2756)G of methionine synthase, with lowered plasma homocysteine levels
    M Y Tsai
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN, USA
    Atherosclerosis 149:131-7. 2000
  4. ncbi Polymorphisms in genes involved in folate metabolism and colorectal neoplasia: a HuGE review
    Linda Sharp
    Epidemiology Group, Department of Medicine and Therapeutics, University of Aberdeen, Scotland, UK
    Am J Epidemiol 159:423-43. 2004
  5. ncbi Homocysteine remethylation enzyme polymorphisms and increased risks for neural tube defects
    Huiping Zhu
    Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, TX 77030, USA
    Mol Genet Metab 78:216-21. 2003
  6. ncbi Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders
    D Leclerc
    MRC Group in Medical Genetics, Department of Pediatrics, McGill University, Montreal, Quebec, Canada
    Hum Mol Genet 5:1867-74. 1996
  7. ncbi Cloning and characterization of a cDNA encoding a cobalamin-independent methionine synthase from potato (Solanum tuberosum L.)
    Michaela Zeh
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Golm, Germany
    Plant Mol Biol 48:255-65. 2002
  8. ncbi The folate pool in colorectal cancers is associated with DNA hypermethylation and with a polymorphism in methylenetetrahydrofolate reductase
    Kazuyuki Kawakami
    Department of Surgery, Kanazawa University School of Medicine, Kanazawa, Japan
    Clin Cancer Res 9:5860-5. 2003
  9. ncbi Relationship between total plasma homocysteine, polymorphisms of homocysteine metabolism related enzymes, risk factors and coronary artery disease in the Australian hospital-based population
    X L Wang
    Department of Cardiovascular Medicine, University of New South Wales, Prince Henry Prince of Wales Hospitals, Sydney, Australia
    Atherosclerosis 146:133-40. 1999
  10. ncbi Polymorphisms of key enzymes in homocysteine metabolism affect diet responsiveness of plasma homocysteine in healthy women
    M L Silaste
    Department of Internal Medicine, University of Oulu, Kajaanintie 50, FIN 90220 Oulu, Finland
    J Nutr 131:2643-7. 2001

Detail Information

Publications213 found, 100 shown here

  1. ncbi Methionine synthase D919G polymorphism, folate metabolism, and colorectal adenoma risk
    Ellen L Goode
    Cancer Prevention Program, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cancer Epidemiol Biomarkers Prev 13:157-62. 2004
    ..These findings add to what is known about the complexities of genetic variations in one-carbon-metabolizing enzymes in relation to colorectal carcinogenesis...
  2. ncbi Influence of a methionine synthase (D919G) polymorphism on plasma homocysteine and folate levels and relation to risk of myocardial infarction
    J Chen
    Department of Epidemiology, Harvard School of Public Health, Boston, MA, USA
    Atherosclerosis 154:667-72. 2001
    ..It was concluded that influence of the MS (D919G) polymorphism on the plasma tHcy and folate levels is at most moderate, but should be further investigated in other large prospective studies...
  3. ncbi Polygenic influence on plasma homocysteine: association of two prevalent mutations, the 844ins68 of cystathionine beta-synthase and A(2756)G of methionine synthase, with lowered plasma homocysteine levels
    M Y Tsai
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN, USA
    Atherosclerosis 149:131-7. 2000
    ....
  4. ncbi Polymorphisms in genes involved in folate metabolism and colorectal neoplasia: a HuGE review
    Linda Sharp
    Epidemiology Group, Department of Medicine and Therapeutics, University of Aberdeen, Scotland, UK
    Am J Epidemiol 159:423-43. 2004
    ..Overall, the roles of folate-pathway genes, folate, and related dietary factors in colorectal neoplasia are complex. Research priorities are suggested...
  5. ncbi Homocysteine remethylation enzyme polymorphisms and increased risks for neural tube defects
    Huiping Zhu
    Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, TX 77030, USA
    Mol Genet Metab 78:216-21. 2003
    ..These results did not appear to be influenced by maternal periconceptional folic acid intake. However,the sample size of this study was limited, and a larger population study is needed to pursue these initial observations...
  6. ncbi Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders
    D Leclerc
    MRC Group in Medical Genetics, Department of Pediatrics, McGill University, Montreal, Quebec, Canada
    Hum Mol Genet 5:1867-74. 1996
    ..We discuss the possibility that a mild deficiency of methionine synthase activity could be associated with mild hyperhomocysteinemia, a risk factor for cardiovascular disease and possibly neural tube defects...
  7. ncbi Cloning and characterization of a cDNA encoding a cobalamin-independent methionine synthase from potato (Solanum tuberosum L.)
    Michaela Zeh
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Golm, Germany
    Plant Mol Biol 48:255-65. 2002
    ..This experiment confirmed the presence of a day/night rhythm. Methionine synthase expression is regulated by photoassimilates but this seems not to detectably alter protein levels...
  8. ncbi The folate pool in colorectal cancers is associated with DNA hypermethylation and with a polymorphism in methylenetetrahydrofolate reductase
    Kazuyuki Kawakami
    Department of Surgery, Kanazawa University School of Medicine, Kanazawa, Japan
    Clin Cancer Res 9:5860-5. 2003
    ....
  9. ncbi Relationship between total plasma homocysteine, polymorphisms of homocysteine metabolism related enzymes, risk factors and coronary artery disease in the Australian hospital-based population
    X L Wang
    Department of Cardiovascular Medicine, University of New South Wales, Prince Henry Prince of Wales Hospitals, Sydney, Australia
    Atherosclerosis 146:133-40. 1999
    ..The contributions to total plasma homocysteine levels of the common mutations of genes coding for the enzymes controlling homocysteine metabolism are modest...
  10. ncbi Polymorphisms of key enzymes in homocysteine metabolism affect diet responsiveness of plasma homocysteine in healthy women
    M L Silaste
    Department of Internal Medicine, University of Oulu, Kajaanintie 50, FIN 90220 Oulu, Finland
    J Nutr 131:2643-7. 2001
    ..The 844ins68 of CBS gene did not affect plasma tHcy concentrations or diet responsiveness. In conclusion, diet responsiveness of plasma homocysteine may be genetically regulated...
  11. pmc Cloning and mapping of a cDNA for methionine synthase reductase, a flavoprotein defective in patients with homocystinuria
    D Leclerc
    Medical Research Council Group in Medical Genetics, The Montreal Children s Hospital, McGill University Health Centre, Montreal, PQ, Canada H3Z 2Z3
    Proc Natl Acad Sci U S A 95:3059-64. 1998
    ..The cloning of the cDNA will permit the diagnostic characterization of cblE patients and investigation of the potential role of polymorphisms of this enzyme as a risk factor in hyperhomocysteinemia-linked vascular disease...
  12. ncbi Association between polymorphisms of folate- and methionine-metabolizing enzymes and susceptibility to malignant lymphoma
    K Matsuo
    Division of Epidemiology and Prevention, the Department of Hematology and Chemotherapy, the Nagoya University Graduate School of Medicine, Japan
    Blood 97:3205-9. 2001
    ..Further studies to confirm the association and detailed biologic mechanisms are now required...
  13. ncbi Study of MTHFR and MS polymorphisms as risk factors for NTD in the Italian population
    Patrizia De Marco
    Istituto G Gaslini, Largo G Gaslini, 5 16148 Genova, Italy
    J Hum Genet 47:319-24. 2002
    ..This study shows that the MTHFRA1298C polymorphism is a genetic determinant for NTD risk in Italy. No association between the MSA2756G and NTD susceptibility was found...
  14. ncbi Genetic causes of mild hyperhomocysteinemia in patients with premature occlusive coronary artery diseases
    M Y Tsai
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455, USA
    Atherosclerosis 143:163-70. 1999
    ....
  15. ncbi Genetic determinants of folate and vitamin B12 metabolism: a common pathway in neural tube defect and Down syndrome?
    Jean Louis Gueant
    INSERM 00 14, Laboratory of Cellular and Molecular Pathology in Nutrition, Faculty of Medicine Nancy, Vandoeuvre les Nancy, France
    Clin Chem Lab Med 41:1473-7. 2003
    ..The distinct data produced in different geographical areas may be explained by differences in the nutritional environment and genetic characteristics of the populations...
  16. ncbi Homozygous VN (677C to T) and d/D (2756G to A) variants in the methylenetetrahydrofolate and methionine synthase genes in a case of hyperhomocysteinemia with stroke at young age
    K S Song
    Department of Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea
    Exp Mol Med 33:106-9. 2001
    ..We report here on a case of occlusive stroke at young age and hyperhomocysteinemia with homozygous VN (677C to T) variant in the MTHFR gene as well as homozygous D/D (2756G to A) variant in the MS gene...
  17. ncbi Gene polymorphisms of homocysteine metabolism-related enzymes in Chinese patients with occlusive coronary artery or cerebral vascular diseases
    G Zhang
    Laboratory of Molecular Hematology, Division of Hematology, The Second Affiliated Hospital, Hunan Medical University, Changsha, Hunan 410011, China
    Thromb Res 104:187-95. 2001
    ..However, CBS 844ins68 may be a protective factor against vascular thromboembolic disease. The prevalence of CBS 844ins68 and MS A2756G in Chinese population is obviously lower than in Western Caucasian population...
  18. ncbi The 2756A>G variant in the gene encoding methionine synthase: its relation with plasma homocysteine levels and risk of coronary heart disease in a Dutch case-control study
    Mariska Klerk
    Division of Human Nutrition and Epidemiology, Wageningen University, Wageningen, The Netherlands
    Thromb Res 110:87-91. 2003
    ..A 2756A>G polymorphism has been found in the gene (MTR) coding for methionine synthase, an enzyme catalyzing remethylation of homocysteine to methionine...
  19. ncbi Interaction between common folate polymorphisms and B-vitamin nutritional status modulates homocysteine and risk for a thrombotic event
    Zoe Yates
    Academic Unit of Paediatrics and Obstetrics and Gynaecology, D Floor, Clarendon Wing, Leeds General Infirmary, University of Leeds, Leeds, West Yorkshire LS2 9NS, UK
    Mol Genet Metab 79:201-13. 2003
    ....
  20. ncbi Methionine synthase genetic polymorphism MS A2756G alters susceptibility to follicular but not diffuse large B-cell non-Hodgkin's lymphoma or multiple myeloma
    Lisa F Lincz
    Hunter Haematology Research Group, Newcastle Mater Misericordiae Hospital, NSW, Australia
    Br J Haematol 120:1051-4. 2003
    ..4-fold lower risk of follicular (odds ratio = 0.41, 95% confidence interval: 0.19-0.88, p = 0.02) but not diffuse large B-cell lymphoma. MM patients showed no significant difference in the polymorphisms compared with control subjects...
  21. ncbi Homocysteine concentrations in adults with trisomy 21: effect of B vitamins and genetic polymorphisms
    Nathalie Fillon-Emery
    Faculte de Medecine, Laboratory of Medical Biochemistry, Vandoeuvre les Nancy, France
    Am J Clin Nutr 80:1551-7. 2004
    ..The effects of supplementation with B vitamins and of common polymorphisms in genes involved in homocysteine metabolism on plasma total homocysteine (tHcy) concentrations in trisomy 21 are unknown...
  22. ncbi One-carbon metabolism-related gene polymorphisms and risk of head and neck squamous cell carcinoma: case-control study
    Takeshi Suzuki
    Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    Cancer Sci 98:1439-46. 2007
    ..04), MTR A2756G (P = 0.04) and MTRR A66G (P = 0.03) polymorphisms. The results suggest that there may be interactions between one-carbon metabolism-related polymorphisms and alcohol drinking for HNSCC risk...
  23. ncbi Methionine synthase (MTR) 2756 (A --> G) polymorphism, double heterozygosity methionine synthase 2756 AG/methionine synthase reductase (MTRR) 66 AG, and elevated homocysteinemia are three risk factors for having a child with Down syndrome
    Paolo Bosco
    IRCCS, Oasi Maria S S Institute for Research on Mental Retardation and Brain Aging, Troina EN, Italy
    Am J Med Genet A 121:219-24. 2003
    ..0 (95% CI: 1.1-24.1), after adjustment for t-Hcys. In conclusion, our results provide evidences that homocysteine and MTR genetic polymorphism are two potent risk factors for mothers to have a DS child in Sicily...
  24. ncbi Effects of polymorphisms of methionine synthase and methionine synthase reductase on total plasma homocysteine in the NHLBI Family Heart Study
    Paul F Jacques
    Jean Mayer USDA, Human Nutrition Research Center on Aging at Tufts University, 711 Wasington Street, Boston, MA 02111, USA
    Atherosclerosis 166:49-55. 2003
    ..This study provides no evidence that these common MTR and MTRR mutations are associated with alterations in plasma homocysteine...
  25. ncbi Adverse effect of nitrous oxide in a child with 5,10-methylenetetrahydrofolate reductase deficiency
    Rebecca R Selzer
    Department of Anesthesiology, University of Wisconsin Medical School, Madison, USA
    N Engl J Med 349:45-50. 2003
  26. ncbi Polymorphisms in the thymidylate synthase and serine hydroxymethyltransferase genes and risk of adult acute lymphocytic leukemia
    Christine F Skibola
    NFCR Center for Genomics and Nutrition, School of Public Health, and the Department of Nutritional Sciences, University of California, Berkeley, CA 94720 7360, USA
    Blood 99:3786-91. 2002
    ....
  27. ncbi [Genetic risk factors of neural tube defects]
    Elzbieta Sliwerska
    Zaklad Genetyki Medycznej, Instytut Matki i Dziecka, Kasprzaka 17a, 01 211 Warszawa, Poland
    Med Wieku Rozwoj 6:349-70. 2002
    ..Ones of those are genes of metabolism of folic acid as MTHFR, MTR, MTRR, CBS, MTHFD, folic acid receptors (FR) regulator genes from PAX family, T, PDGFRA and BRCA1 genes...
  28. ncbi Genetic and nutritional factors contributing to hyperhomocysteinemia in young adults
    Leo A J Kluijtmans
    Department of Pharmacology and Center for Pharmacogenetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Blood 101:2483-8. 2003
    ..These data underscore the potential benefits that may be gained by improving the dietary status of young adults, and provide support for the implementation of folate/B-vitamin food fortification programs...
  29. ncbi Homocysteine concentrations and molecular analysis in patients with congenital heart defects
    Luciano C Galdieri
    Department of Pediatrics, Universidade Federal de Sao Paulo UNIFESP EPM, Sao Paulo, Brazil
    Arch Med Res 38:212-8. 2007
    ..Congenital heart defects are the result of incomplete heart development and, like many diseases, have been associated with high homocysteine concentration...
  30. ncbi Effects of folic acid fortification and multivitamin therapy on homocysteine and vitamin B(12) status in cardiac transplant recipients
    Santiago G Miriuka
    Department of Medicine, University of Toronto, Toronto, Ontario, Canada
    J Heart Lung Transplant 23:405-12. 2004
    ..In 1998, food in Canada was fortified nationwide with folic acid. We assessed the impact of routine folate fortification on homocysteine concentrations in our cardiac transplant population...
  31. ncbi [Relations of methionine synthase gene variation with congenital heart disease]
    Wenli Zhu
    School of Public Health, Peking University Health Science Center, Beijing 100083, China
    Wei Sheng Yan Jiu 33:66-9. 2004
    ..Methionine synthase (MS) is the key enzyme in the homocysteine metabolism. To investigate the relations of MS gene variation with occurrence of congenital heart disease (CHD)...
  32. ncbi The methionine synthase polymorphism c.2756A>G alters susceptibility to glioblastoma multiforme
    Alexander Semmler
    Department of Neurology, University Hospital Bonn, Sigmund Freud Strasse, 25, 53105 Bonn, Germany
    Cancer Epidemiol Biomarkers Prev 15:2314-6. 2006
    ..72/0.26/0.02 and controls AA/AG/GG: 0.57/0.38/0.05, degrees of freedom = 2; chi(2) = 17.86 (Pearson); P < 0.001]. No association between glioblastoma multiforme and the two other polymorphisms was observed...
  33. ncbi Polymorphisms of genes controlling homocysteine levels and IQ score following the treatment for childhood ALL
    Maja Krajinovic
    Hopital Sainte Justine, Service d hématologie oncologie, Centre de Recherche, 3175 Cote Sainte Catherine, Montreal, Quebec H3T 1C5, Canada
    Pharmacogenomics 6:293-302. 2005
    ..Several enzymes are essential to maintain the homocysteine levels. Their different functional forms, associated with common genetic polymorphisms, may modulate homocysteine levels and thereby influence MTX-associated neurotoxicity...
  34. ncbi No association between common polymorphisms in genes of folate and homocysteine metabolism and the risk of Down's syndrome among French mothers
    Abalo Chango
    INSERM U 724, Laboratory of Nutritional Genomics, ISAB Agrohealth, 60026 Beauvais, France
    Br J Nutr 94:166-9. 2005
    ..The risk of having a child with trisomy 21 did not appear to be linked to polymorphisms in genes associated with folate and homocysteine metabolism...
  35. ncbi Abnormal folate metabolism in mothers with Down syndrome offspring: review of the literature
    T K A B Eskes
    University Medical Center St Radboud, 6500 HB Nijmegen NL, Netherlands
    Eur J Obstet Gynecol Reprod Biol 124:130-3. 2006
    ..This finding, in addition to the prevention of neural tube defects, strengthens the recommendation to use folic acid around conception...
  36. ncbi Polymorphisms in folate metabolizing genes and risk for spontaneous preterm and small-for-gestational age birth
    Stephanie M Engel
    Department of Community and Preventive Medicine, Mount Sinai School of Medicine, New York, NY, USA
    Am J Obstet Gynecol 195:1231.e1-11. 2006
    ..Variants in the folate metabolism pathway affect the accumulation of homocysteine are modified by nutrient levels and have been linked to adverse birth outcomes...
  37. ncbi Study of four genes belonging to the folate pathway: transcobalamin 2 is involved in the onset of non-syndromic cleft lip with or without cleft palate
    Marcella Martinelli
    Department of Morphology and Embryology, University of Ferrara, Ferrara, Italy
    Hum Mutat 27:294. 2006
    ....
  38. ncbi Influence of methionine synthase (A2756G) and methionine synthase reductase (A66G) polymorphisms on plasma homocysteine levels and relation to risk of coronary artery disease
    Abdelilah Laraqui
    Ligue nationale de lutte contre les maladies cardiovasculaires, Laboratoire de Biochimie et de Biologie Moleculaire, CHU Avicenne, Rabat, Morocco
    Acta Cardiol 61:51-61. 2006
    ..We also determined the genotype frequencies distribution of the A2756G transition of the B12-dependent methionine synthase (MTR) gene and the A66G mutation of the methionine synthase reductase (MTRR) gene...
  39. ncbi Vitamin B12 and methionine synthesis: a critical review. Is nature's most beautiful cofactor misunderstood?
    John I Toohey
    Cytoregulation Research, Elgin, Ontario, Canada KOG 1EO
    Biofactors 26:45-57. 2006
    ..The rearranged product is readily degraded to CH3-SH, providing a mechanism for removing toxic homocysteine...
  40. ncbi Polymorphisms in genes involved in folate metabolism as risk factors for oedematous severe childhood malnutrition: a hypothesis-generating study
    Kwesi G Marshall
    Tropical Metabolism Research Unit, Tropical Medicine Research Institute, University of the West Indies, Mona, Jamaica
    Ann Trop Paediatr 26:107-14. 2006
    ....
  41. ncbi [Association between genetic polymorphisms in folate metabolic enzyme genes and colorectal cancer: a nested case-control study]
    Kun Chen
    School of Public Health, Zhejiang University, Hangzhou 310031, China
    Zhonghua Zhong Liu Za Zhi 28:429-32. 2006
    ..To investigate the interrelationship of genetic polymorphisms in folate metabolic enzymes (MTHFRC677T, MTHFRA1298C, MTRA2756G and MTRRA66G) and their combinative effects with colorectal cancer (CRC)...
  42. ncbi Relationship between polymorphisms in genes involved in homocysteine metabolism and maternal risk for Down syndrome in Brazil
    Luciana Rodrigues Jacy da Silva
    Centro de Genetica Medica, Departamentos de Morfologia e Pediatria, UNIFESP EPM, Sao Paulo, Brazil
    Am J Med Genet A 135:263-7. 2005
    ..05). However, when the presence of 677T, 1298C, 2756G, 66G, and 844ins68 alleles were evaluated together, the mothers of children with DS tend to have a higher number of uncommon alleles than the mothers with no previous affected child...
  43. ncbi Polymorphisms of genes involved in homocysteine metabolism in preeclampsia and in uncomplicated pregnancies
    Eva Also-Rallo
    Department of Genetics of the University of Barcelona, Spain
    Eur J Obstet Gynecol Reprod Biol 120:45-52. 2005
    ..To evaluate the possible relationship between preeclampsia and polymorphisms in the main genes involved in folate-homocysteine metabolism...
  44. ncbi Polymorphisms of folate metabolic genes and susceptibility to bladder cancer: a case-control study
    Jie Lin
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Carcinogenesis 25:1639-47. 2004
    ..These results have important implications for cancer prevention in susceptible populations...
  45. pmc The methionine synthase polymorphism D919G alters susceptibility to primary central nervous system lymphoma
    M Linnebank
    Department of Neurology, University Hospital of Bonn, Sigmund Freud Str 25, Bonn D 53125, Germany
    Br J Cancer 90:1969-71. 2004
    ..26, CI(95%): 0.09-0.74; P=0.005), suggesting a protective function of the G allele. These data stimulate further epidemiological and functional studies focusing on the role of homocysteine and folate metabolism in lymphoma tumorigenesis...
  46. ncbi Common gene polymorphisms in the metabolic folate and methylation pathway and the risk of acute lymphoblastic leukemia and non-Hodgkin's lymphoma in adults
    Donato Gemmati
    Department of Biomedical Sciences and Advanced Therapies, Center Study for Hemostasis and Thrombosis, University of Ferrara, C so Giovecca 203, I 44100 Ferrara, Italy
    Cancer Epidemiol Biomarkers Prev 13:787-94. 2004
    ..These data are in accordance with the hypothesis that polymorphisms in the genes for folate and methionine metabolism might play a greater role in the occurrence of ALL than NHL by influencing DNA synthesis and/or DNA methylation...
  47. ncbi Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate
    Jean Luc Ferrer
    Laboratoire de Cristallogenèse et Cristallographie des Protéines, Institut de Biologie Structurale J P Ebel, 41 rue Jules Horowitz, 38027 Grenoble 1, France
    J Biol Chem 279:44235-8. 2004
    ....
  48. ncbi Lack of genetic dispositions to hyperhomocysteinemia in Alzheimer disease
    Michael Linnebank
    Am J Med Genet A 131:101-2. 2004
  49. ncbi Genetic polymorphisms predisposing to hyperhomocysteinemia in cardiac transplant patients
    Santiago G Miriuka
    Department of Medicine, Toronto General Hospital and University of Toronto, Toronto, Ontario, Canada
    Transpl Int 18:29-35. 2005
    ..Thus, SNP in Hcy-regulating genes may be important determinants of vitamin metabolism in CTX, raising the question of increased vitamin requirements to minimize increased plasma Hcy in this high-risk group...
  50. ncbi Genetic polymorphisms in the one-carbon metabolism pathway and breast cancer risk: a population-based case-control study and meta-analyses
    Jolanta Lissowska
    Department of Cancer Epidemiology and Prevention, Cancer Center and M Sklodowska Curie Institute of Oncology, Warsaw, Poland
    Int J Cancer 120:2696-703. 2007
    ..In conclusion, this report does not support a substantial overall association between the evaluated polymorphisms in the one-carbon metabolism pathway and breast cancer risk...
  51. ncbi cblE type of homocystinuria due to methionine synthase reductase deficiency: functional correction by minigene expression
    Petra Zavadakova
    Institute of Inherited Metabolic Diseases, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Hum Mutat 25:239-47. 2005
    ..The identification of mutations in the MTRR gene, together with restoration of methionine synthesis following MTRR minigene expression in cblE cells confirms that this disease is caused by defects in the MTRR gene...
  52. ncbi Expression profiling of homocysteine junction enzymes in the NCI60 panel of human cancer cell lines
    Wen Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    Cancer Res 65:1554-60. 2005
    ....
  53. ncbi Genetic polymorphism of folate and methionine metabolizing enzymes and their susceptibility to malignant lymphoma
    Emmad Ezzat Habib
    The Department of Clinical Oncology, Cairo University Medical School, Clinical Pathology, Faculty of Medicine, Cairo University
    J Egypt Natl Canc Inst 17:184-92. 2005
    ..The MS 2756 A-->G polymorphism also reduces the enzyme activity and results in the hypomethylation of DNA...
  54. ncbi Association between polymorphisms of folate-metabolizing enzymes and risk of prostate cancer
    C Marchal
    Urology Department, Hospital Clinico Universitario, Virgen de la Victoria, Campus de Teatinos s n, Malaga 29010, Spain
    Eur J Surg Oncol 34:805-10. 2008
    ....
  55. ncbi Are genetic variants of the methyl group metabolism enzymes risk factors predisposing to obesity?
    I Terruzzi
    Division of Internal Medicine, Section of Nutrition Metabolism, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Endocrinol Invest 30:747-53. 2007
    ....
  56. ncbi Hyperhomocysteinemia is related to residual glomerular filtration and folate, but not to methylenetetrahydrofolate-reductase and methionine synthase polymorphisms, in supplemented end-stage renal disease patients undergoing hemodialysis
    W Anwar
    Laboratoire de Pathologie Cellulaire et Moléculaire en Nutrition EMI INSERM 0014 et URM IFREMER 20, Faculte de Medecine, Vandoeuvre, France
    Clin Chem Lab Med 39:747-52. 2001
    ..These data suggest that hyperhomocysteinemia is a consequence as well as a complicating factor of renal failure...
  57. ncbi Prevalence and mechanisms of hyperhomocysteinemia in chronic alcoholics
    Carmen Blasco
    Liver Unit, Institut de Malalties Digestives i Metaboliques, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, University of Barcelona, Barcelona, Spain
    Alcohol Clin Exp Res 29:1044-8. 2005
    ..The aim of the study was to investigate the prevalence of hyperhomocysteinemia in chronic alcoholics and the influence of alcohol consumption, vitamin deficiencies and liver damage on the plasma levels of Hcy...
  58. ncbi Genetic determinants of methotrexate toxicity in rheumatoid arthritis patients: a study of polymorphisms affecting methotrexate transport and folate metabolism
    Petra Bohanec Grabar
    Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia
    Eur J Clin Pharmacol 64:1057-68. 2008
    ....
  59. ncbi Functional polymorphisms in folate metabolism genes influence the risk of meningioma and glioma
    Lara Bethke
    Section of Cancer Genetics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom
    Cancer Epidemiol Biomarkers Prev 17:1195-202. 2008
    ..94). Our findings provide support for the role of folate metabolism in the development of primary brain tumors. In particular, genotypes associated with increased 5,10-methylenetetrahydrofolate levels are associated with elevated risk...
  60. ncbi A common variant in methionine synthase reductase combined with low cobalamin (vitamin B12) increases risk for spina bifida
    A Wilson
    The Montreal Children s Hospital Research Institute, McGill University, Montreal, Quebec, Canada
    Mol Genet Metab 67:317-23. 1999
    ..Investigation of this polymorphism in other disorders associated with altered homocysteine metabolism, such as vascular disease, is clearly warranted...
  61. ncbi Molecular basis for methionine synthase reductase deficiency in patients belonging to the cblE complementation group of disorders in folate/cobalamin metabolism
    A Wilson
    MRC Group in Medical Genetics, Montreal Children s Hospital, Canada
    Hum Mol Genet 8:2009-16. 1999
    ..These data demonstrate a unique requirement for MSR in the reductive activation of MS...
  62. ncbi Gene-environment and gene-gene interaction in the determination of plasma homocysteine levels in healthy middle-aged men
    V Dekou
    Cardiovascular Genetics, Department of Medicine, University College London Medical School, The Rayne Institute, UK
    Thromb Haemost 85:67-74. 2001
    ....
  63. ncbi Polymorphisms in genes related to folate and cobalamin metabolism and the associations with complex birth defects
    R Brouns
    Department of Clinical Chemistry, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
    Prenat Diagn 28:485-93. 2008
    ..To investigate the associations between biomarkers and genetic variants involved in homocysteine metabolism and the risk of complex birth defects...
  64. ncbi Effect of heterozygosity for the methionine synthase 2756 A-->G mutation on the risk for recurrent cardiovascular events
    M E Hyndman
    Department of Medical Genetics, University of Calgary, Alberta, Canada
    Am J Cardiol 86:1144-6, A9. 2000
    ..This mutation significantly reduced the risk for recurrent cardiovascular events and elevated red blood cell folate levels...
  65. ncbi Increased prevalence of combined MTR and MTHFR genotypes among individuals with severely elevated total homocysteine plasma levels
    A Feix
    Department of Medicine III, Division of Nephrology and Dialysis, Division of Endocrinology and Metabolism, University of Vienna, Austria
    Am J Kidney Dis 38:956-64. 2001
    ..0155; OR, 0.330; 95% CI, 0.126 to 0.861). In summary, our study shows that the 2756A-->G transition of MTR in combination with MTHFR 677TT/1298AA and 677CT/1298AC can be associated with extremely high tHcy plasma levels...
  66. ncbi Methylenetetrahydrofolate reductase 677 T allele protects against persistent HBV infection in West Africa
    Jean Pierre Bronowicki
    INSERM U724, Cellular and Molecular Pathology in Nutrition, Faculte de Medecine, University Henri Poincaré of Nancy, Vandoeuvre les Nancy, France
    J Hepatol 48:532-9. 2008
    ..We evaluated the association of determinants of homocysteine metabolism with the outcome of HBV infection...
  67. ncbi One-carbon metabolism-related gene polymorphisms and risk of breast cancer
    Takeshi Suzuki
    Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    Carcinogenesis 29:356-62. 2008
    ..008). Our findings indicated that the MTHFR and MTRR polymorphisms were associated with individual susceptibility to breast cancer among postmenopausal women...
  68. ncbi Relationship between methionine synthase, methionine synthase reductase genetic polymorphisms and deep vein thrombosis among South Indians
    Shaik Mohammad Naushad
    Diagnostic Division, Center for DNA Fingerprinting and Diagnostics, Hyderabad, Andhra Pradesh, India
    Clin Chem Lab Med 46:73-9. 2008
    ....
  69. ncbi Essential hypertension in adolescents: association with insulin resistance and with metabolism of homocysteine and vitamins
    Regina Kahleová
    Institute of Inherited Metabolic Disease, Charles University, 1st Faculty of Medicine, Prague, Czech Republic
    Am J Hypertens 15:857-64. 2002
    ..We examined whether insulin resistance and disturbed homocysteine metabolism are present in young adults at the early stages of essential hypertension...
  70. pmc Sinorhizobium meliloti requires a cobalamin-dependent ribonucleotide reductase for symbiosis with its plant host
    Michiko E Taga
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Mol Plant Microbe Interact 23:1643-54. 2010
    ..These results show that the critical role of the cobalamin-dependent RNR for survival of S. meliloti in its plant host can account for the considerable resources that S. meliloti dedicates to cobalamin biosynthesis...
  71. pmc Oxidative stress inactivates cobalamin-independent methionine synthase (MetE) in Escherichia coli
    Elise R Hondorp
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA
    PLoS Biol 2:e336. 2004
    ..In eukaryotes, glutathionylation of key proteins involved in protein synthesis leads to inhibition of translation. Our studies suggest a simpler mechanism is employed by E. coli to achieve the same effect...
  72. ncbi [A rare inborn error of intracellular processing of cobalamine presenting with microcephalus and megaloblastic anemia: a report of 3 children]
    P Muller
    HELIOS Krankenhaus Leisnig, Abteilung für Kinder und Jugendmedizin, Leisning
    Klin Padiatr 219:361-7. 2007
    ..Patients present with megaloblastic anemia, failure to thrive and various neurological manifestations including mental retardation, cerebral atrophy, muscular hypotonia or hypertonia, ataxia, seizures, nystagmus and visual disturbances...
  73. ncbi The role of folic acid and Vitamin B12 in genomic stability of human cells
    M Fenech
    CSIRO Health Sciences and Nutrition, Adelaide, Australia
    Mutat Res 475:57-67. 2001
    ..Dietary intakes above the current RDI may be particularly important in those with extreme defects in the absorption and metabolism of these Vitamins, for which ageing is a contributing factor...
  74. ncbi MTHFD 1958G>A and MTR 2756A>G polymorphisms are associated with bipolar disorder and schizophrenia
    Bartosz Kempisty
    Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland
    Psychiatr Genet 17:177-81. 2007
    ....
  75. ncbi Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry
    Kirsten R Wolthers
    Faculty of Life Sciences, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, United Kingdom
    Biochemistry 46:11833-44. 2007
    ..The biological implications of an attenuated mechanism of MS reactivation by MSR on methionine and folate metabolism are discussed...
  76. pmc Comparative genomic analyses of nickel, cobalt and vitamin B12 utilization
    Yan Zhang
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, NE 68588 0664, USA
    BMC Genomics 10:78. 2009
    ..Although a number of Ni and Co-dependent enzymes have been characterized, systematic evolutionary analyses of utilization of these metals are limited...
  77. ncbi Alcoholic liver disease and methionine metabolism
    Kusum K Kharbanda
    Department of Veterans Affairs Medical Center, Research Service, Omaha, Nebraska 68105, USA
    Semin Liver Dis 29:155-65. 2009
    ..Thus, betaine is a promising therapeutic agent in relieving the methylation and other defects associated with alcoholic abuse...
  78. ncbi Genetic determinants of hyperhomocysteinaemia: the roles of cystathionine beta-synthase and 5,10-methylenetetrahydrofolate reductase
    H J Blom
    Department of Paediatrics, University Hospital Nijmegen, The Netherlands
    Eur J Pediatr 159:S208-12. 2000
    ..The elevated Hcy levels due to these mutations can be normalised by administration of folate, but whether folate reduces the risk of cardiovascular disease remains to be established...
  79. ncbi High prevalence of hyperhomocysteinemia related to folate deficiency and the 677C-->T mutation of the gene encoding methylenetetrahydrofolate reductase in coastal West Africa
    Emile K Amouzou
    Laboratory of Cellular and Molecular Pathology in Nutrition, EMI INSERM 00 14, Vandoeuvre les Nancy, France
    Am J Clin Nutr 79:619-24. 2004
    ..Moderate hyperhomocysteinemia is a risk for neural tube defect and neurodegenerative and vascular diseases and has nutritional, metabolic, and genetic determinants. Its prevalence in sub-Saharan Africa remains unknown...
  80. ncbi Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395
    Adrian J Dunford
    Department of Biochemistry, University of Leicester, UK
    Eur J Biochem 271:2548-60. 2004
    ..In wild-type and F1395W FAD domains, prolonged incubation with NADPH results in development of the neutral blue semiquinone FAD species. This reaction is suppressed in the mutant FAD domains lacking the shielding aromatic residue...
  81. ncbi Large-scale population-based metabolic phenotyping of thirteen genetic polymorphisms related to one-carbon metabolism
    Ase Fredriksen
    LOCUS for Homocysteine and Related Vitamins, University of Bergen, Bergen, Norway
    Hum Mutat 28:856-65. 2007
    ..2756A>G (tHcy), BHMT c.716G>A (DMG), CBS c.844_845ins68 (tHcy, betaine), CBS c.699C>T (tHcy, betaine, cystathionine) and TCN2 c.776C>G (MMA). No associations were observed for the other polymorphisms investigated...
  82. ncbi Marfanoid features in a child with combined methylmalonic aciduria and homocystinuria (CblC type)
    Sandra G Heil
    Department of Pediatrics, Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB, Nijmegen, The Netherlands
    J Inherit Metab Dis 30:811. 2007
    ....
  83. ncbi Role of parental folate pathway single nucleotide polymorphisms in altering the susceptibility to neural tube defects in South India
    Shaik Mohammad Naushad
    Division of Diagnostic, Center for DNA Fingerprinting and Diagnostics, Hyderabad, India
    J Perinat Med 38:63-9. 2010
    ....
  84. ncbi Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase
    Horatiu Olteanu
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:1988-97. 2004
    ..It is likely that differences in their relative affinities for the redox partner, methionine synthase, underlie the differences in the relative efficiencies of reductive activation exhibited by the variants...
  85. ncbi Methionine synthase reductase 66A->G polymorphism is associated with increased plasma homocysteine concentration when combined with the homozygous methylenetetrahydrofolate reductase 677C->T variant
    Jaimie D Vaughn
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 134:2985-90. 2004
    ....
  86. ncbi MTRR 66A>G polymorphism in relation to congenital heart defects
    Ingrid M van Beynum
    Children s Heart Centre, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Chem Lab Med 44:1317-23. 2006
    ..In addition, possible interaction between this variant and plasma methylmalonic acid (MMA) concentrations, as an indicator of intracellular vitamin B(12) status, was investigated...
  87. ncbi Correction of the DNA synthesis defect in vitamin B12 deficiency by tetrahydrofolate: evidence in favour of the methyl-folate trap hypothesis as the cause of megaloblastic anaemia in vitamin B12 deficiency
    A V Hoffbrand
    Department of Haematology, Royal Free Hospital, School of Medicine, London
    Br J Haematol 83:643-7. 1993
    ..The results therefore favour the theory that it is in the supply of THF and not of 'active formate' or formyl THF that vitamin B12 plays a critical role in folate metabolism...
  88. ncbi Genetic and lifestyle factors related to the periconception vitamin B12 status and congenital heart defects: a Dutch case-control study
    Anna C Verkleij-Hagoort
    Department of Obstetrics, Division of Obstetrics and Prenatal Medicine, Erasmus MC, University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Mol Genet Metab 94:112-9. 2008
    ..The future enlargement of our sample size might demonstrate significant associations...
  89. ncbi CblE type of homocystinuria due to methionine synthase reductase deficiency: clinical and molecular studies and prenatal diagnosis in two families
    P Zavadakova
    Institute of Inherited Metabolic Diseases, Charles University, 1st Faculty of Medicine, Prague, Czech Republic
    J Inherit Metab Dis 25:461-76. 2002
    ..Our study expands the knowledge of the phenotypic and genotypic variability of the cblE type of homocystinuria and supports the concept that this disorder is caused by mutations in the MTRR gene...
  90. pmc Methionine synthase reductase deficiency results in adverse reproductive outcomes and congenital heart defects in mice
    Liyuan Deng
    Department of Human Genetics, McGill University Montreal Children s Hospital Research Institute, Montreal, Canada
    Mol Genet Metab 94:336-42. 2008
    ..These findings may have implications for nutritional prevention of heart defects, particularly in women with the common MTRR polymorphism...
  91. pmc Antisense inhibition of methylenetetrahydrofolate reductase reduces survival of methionine-dependent tumour lines
    J Sekhon
    Department of Human Genetics, McGill University Health Centre Montreal Children s Hospital, 4060 Ste Catherine West, Room 200, Montreal, Quebec H3Z 2Z3, Canada
    Br J Cancer 87:225-30. 2002
    ..This study suggests that methylenetetrahydrofolate reductase may be required for tumour cell survival and that methylenetetrahydrofolate reductase inhibition should be considered for anti-tumour therapy...
  92. ncbi Polymorphic background of methionine synthase reductase modulates the phenotype of a disease-causing mutation
    Carmen Gherasim
    Redox Biology Center and Biochemistry Department, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
    Hum Mutat 28:1028-33. 2007
    ..Val56Met mutation and the p.Ile22Met variation, which was confirmed by sequence analysis. This study reveals how a genetic variation can modulate phenotypic expression of a disease-causing mutation...
  93. ncbi Methionine metabolism in plants: chloroplasts are autonomous for de novo methionine synthesis and can import S-adenosylmethionine from the cytosol
    Stéphane Ravanel
    Departement de Reponse et Dynamique Cellulaires, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
    J Biol Chem 279:22548-57. 2004
    ..Therefore, the chloroplastic AdoMet carrier serves as a link between cytosolic and chloroplastic one-carbon metabolism...
  94. ncbi Prevalence and effects of gene-gene and gene-nutrient interactions on serum folate and serum total homocysteine concentrations in the United States: findings from the third National Health and Nutrition Examination Survey DNA Bank
    Quan He Yang
    National Center on Birth Defects and Developmental Disabilities, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Am J Clin Nutr 88:232-46. 2008
    ..Folate intake and genetic polymorphisms encoding folate-metabolizing enzymes influence blood folate and homocysteine concentrations, but the effects and interactions of these factors have not been studied on a population-wide basis...
  95. pmc Genetic polymorphisms in one-carbon metabolism: associations with CpG island methylator phenotype (CIMP) in colon cancer and the modifying effects of diet
    Karen Curtin
    Department of Internal Medicine, University of Utah Health Sciences Center, 375 Chipeta Way, Suite A, Salt Lake City, UT 84108, USA
    Carcinogenesis 28:1672-9. 2007
    ..1, 95% CI = 1.3-3.4 versus AA/high risk; P-interaction = 0.03). These results provide only limited support for a role of polymorphisms in one-carbon metabolism in the etiology of CIMP colon cancer...
  96. ncbi [Neural tube defects and vitamin B12: a report of three cases]
    M Candito
    Inserm UI 145 et laboratoire de biochimie, Hopital Pasteur, Nice
    Ann Biol Clin (Paris) 62:235-8. 2004
    ..It would therefore be advisable to consider fortifying grain products with both folic acid and vitamin B12...
  97. pmc Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements
    C Melissa Dobson
    Department of Biochemistry and Molecular Biology, University of Calgary, AB, Canada T2N 1N4
    Proc Natl Acad Sci U S A 99:15554-9. 2002
    ..We speculate that we have identified a component of a transporter or an accessory protein that is involved in the translocation of vitamin B(12) into mitochondria...
  98. pmc Sulfur amino acid deficiency upregulates intestinal methionine cycle activity and suppresses epithelial growth in neonatal pigs
    Caroline Bauchart-Thevret
    US Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Physiol Endocrinol Metab 296:E1239-50. 2009
    ..We conclude that SAA deficiency upregulates intestinal methionine cycle activity and suppresses epithelial growth in neonatal pigs...
  99. ncbi A mathematical model of the folate cycle: new insights into folate homeostasis
    H Frederik Nijhout
    Departments of Biology and Mathematics, Duke University, Durham, NC 27708, USA
    J Biol Chem 279:55008-16. 2004
    ..In the presence of folate binding and allosteric inhibition, the velocities show a remarkable constancy as total folate is decreased...
  100. ncbi Betaine lowers elevated s-adenosylhomocysteine levels in hepatocytes from ethanol-fed rats
    Anthony J Barak
    VA Alcohol Research Center, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
    J Nutr 133:2845-8. 2003
    ....
  101. ncbi Cloning and sequencing of the coenzyme B(12)-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli
    A Ratnatilleke
    Institute of Organic Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Biol Chem 274:31679-85. 1999
    ..A V(max) of 38 +/- 3 units/mg IcmA and a k(cat) of 39 +/- 3 s(-1) were determined under saturating molar ratios of IcmB to IcmA...

Research Grants58

  1. The Transcobalamin Receptor in Cobalamin Homeostasis
    Edward V Quadros; Fiscal Year: 2011
    ..abstract_text> ..
  2. Role of homocysteine in retinal disease
    Sylvia B Smith; Fiscal Year: 2013
    ....
  3. B12 Trafficking and Birth Defects
    RUMA V BANERJEE; Fiscal Year: 2013
    ....
  4. MENTORED PATIENT ORIENTED RESEARCH CAREER DEVELOPMENT AW
    MARK STEEN; Fiscal Year: 2001
    ..Third, the CAP will begin the direct study of MS by developing biochemical discriminants for MS carriers in a rare large family with MS deficiency, and will result in the initiation of direct investigation of the enzyme. ..
  5. Pharmacogenetics of Methotrexate Therapy in Arthritis
    ALEXANDER WHITEHEAD; Fiscal Year: 2006
    ..It has the potential to facilitate individualized treatment protocols that are less empirical and therefore more effective, and to reduce the incidence of cardiovascular co-morbidity. ..
  6. RAPID KINETIC AND OTHER STUDIES OF BIOLOGICAL OXYGENASES
    David Ballou; Fiscal Year: 2002
    ..This in turn may lead to the ability to predict how various compounds will be metabolized in the environment. ..
  7. Structural Characterizations of Transient and Heterogeneous Protein Complexes
    Nozomi Ando; Fiscal Year: 2013
    ..This work will be complemented by crystallography, spectroscopy, and analytical ultracentrifugation. ..
  8. GENETIC HETEROGENEITY IN HOMOCYSTEINE REMETHYLATION
    Barry Shane; Fiscal Year: 2000
    ..The long term goals of the project are to delineate the role of genetic heterogeneity in the development of hyperhomocysteinemia and to understand the mechanisms by which homocysteine hemostasis is regulated. (End of Abstract) ..
  9. GENETIC RISK FACTORS FOR HYPERHOMOCYSTEINEMIA
    ROY GRAVEL; Fiscal Year: 2006
    ..In summary, our study will give a biochemical and physiological face to the impact of 122M on enzyme function, development and disease. ..
  10. STRUCTURAL BASIS OF VITAMIN B12 (COBALAMIN) DEFICIENCY
    Heidi Schubert; Fiscal Year: 2002
    ..3) Produce a model of intrinsic factor (IF), haptocorrin and transcobalamin I (TCI) based on the high amino acid sequence identity with TCII. ..
  11. TIME RESOLVED STUDIES OF B12 ENZYMES AN COENZYMES
    ROSEANNE SENSION; Fiscal Year: 2000
    ..In addition the role of the axial histidine ligand supplied by the protein will be probed by using a series of mutations to remove/replace this histidine and to alter the amino acid residues hydrogen bonded to the histidine. ..
  12. Folate, Vitamin D and Calcium in Colorectal Cancer
    Robert Haile; Fiscal Year: 2009
    ..Genotyping will primarily involve SNP-based haplotypes. For both pathways, analyses of gene-gene and gene-environment interactions will be included as appropriate. ..
  13. REGULATION TO FOLATE METABOLISM
    ROWENA MATTHEWS; Fiscal Year: 2000
    ....
  14. NO DAMAGE TO FOLATE CYCLE IN THE CENTRAL NERVOUS SYSTEM
    Kenneth Hensley; Fiscal Year: 2005
    ..abstract_text> ..
  15. Vascular Biochemistry of Vitamin B12
    DONALD JACOBSEN; Fiscal Year: 2006
    ..abstract_text> ..
  16. Relationship of Apo B and BHMT: Nutrition and Physiology
    Janet Sparks; Fiscal Year: 2004
    ..A potential link of two pathways whose products or intermediates are associated with increased cardiovascular disease risk could be established. ..
  17. MECHANISTIC STUDY OF B12-DEPENDENT METHIONINE SYNTHASE
    Vahe Bandarian; Fiscal Year: 2001
    ..These studies may help clarify the utility of the ligand triad in MetH and in an emerging class of B12 dependent enzymes that prefer base-off coordination of the cofactor. ..
  18. FOLATE PATHWAY POLYMORPHISMS AND PANCREATIC CANCER RISK
    Michael Goggins; Fiscal Year: 2002
    ..abstract_text> ..
  19. FOLIC ACID REQUIREMENTS AND ONE CARBON METABOLISM
    Barry Shane; Fiscal Year: 2004
    ....
  20. Homocysteine and Alcoholic Liver Disease
    DONALD WELDON JACOBSEN; Fiscal Year: 2010
    ..This proposal will provide mechanistic insight on the cause of hyperhomocysteinemia and its deleterious effects to tissues in chronic alcohol abuse. ..
  21. Conversion of Inactive Cobalamins to Coenzyme B12
    Thomas Bobik; Fiscal Year: 2005
    ..abstract_text> ..
  22. Genetics of Prostate Cancer Risk & Agressiveness
    Graham Casey; Fiscal Year: 2007
    ..Epidemiologic and clinical data have been collected, as well as biospecimens and banked DNA. The relationship between these genetic factors and prostate cancer risk and aggressiveness will be statistically evaluated. ..
  23. Kinetic Analysis of Methionine Synthase Reductase
    KIRSTEN WOLTHERS; Fiscal Year: 2004
    ..abstract_text> ..
  24. Methylation and Oxidation in Breast Cancer Epidemiology
    Jo Freudenheim; Fiscal Year: 2005
    ....
  25. Folic Acid Enhances Repair Mechanism in the Adult CNS
    Bermans J Iskandar; Fiscal Year: 2010
    ....
  26. DYNAMIC AND KINETIC BEHAVIOR OF FOLATE METABOLISM
    Andrew Clifford; Fiscal Year: 2005
    ..We will determine the in vivo kinetic behavior of a tracer dose of folate in blood, urine, and stool. We will then compare the behavior of folate metabolism (functional endpoint) among the various genotypes. ..
  27. Conformational changes of the multi-modular MetH
    ALLISON LAMANNA; Fiscal Year: 2007
    ..This research will provide insight for the study of additional multi-domain proteins and enzymes. ..
  28. 14th International Congress on Flavins and Flavoproteins
    ROWENA MATTHEWS; Fiscal Year: 2002
    ..Tea will be served at each poster session. Presentations were selected by an International Organizing Committee with an eye to a diverse representation of speakers at the meeting. ..
  29. REGULATION OF FOLATE METABOLISM
    ROWENA MATTHEWS; Fiscal Year: 2008
    ..We predict that phosphorylation will play an important role in modulating the flux of one-carbon units. ..
  30. Studies of Human Folate Hydrolase
    Charles Halsted; Fiscal Year: 2005
    ..abstract_text> ..
  31. SAMe and Folate Deficiency in Alcoholic Mircropigs
    Charles Halsted; Fiscal Year: 2006
    ..While furthering understanding of interactions of methionine metabolites on pathways of liver injury, the project may establish novel approaches to the prevention and treatment of ALD. [unreadable] [unreadable] [unreadable]..
  32. EFFECTS OF SAM IN PATIENTS WITH ALCOHOLIC LIVER DISEASE
    Charles Halsted; Fiscal Year: 2007
    ..By confirming the relationship of abnormal methionine metabolism to clinical, biochemical, and pathologic disease severity, the study will establish a metabolic basis for determining the efficacy of SAM intervention in ALD. ..
  33. A Genome-Wide Association Study of Non-Hodgkin Lymphoma
    Christine F Skibola; Fiscal Year: 2010
    ..The data generated from this study will provide a framework for further investigation within the NHL consortium, InterLymph. Results will later be combined with that from other InterLymph case-control studies in pooled analyses. ..
  34. Omega-3 Fatty Acids in the Treatment of Depression
    David Mischoulon; Fiscal Year: 2005
    ..It is hoped that this project will provide the critical fund of basic knowledge and practical experience necessary to aid the candidate in becoming an independent investigator. ..
  35. Prostaglandin Synthesis, Genetics and Colorectal Cancer
    Cornelia M Ulrich; Fiscal Year: 2010
    ..They will also advance tailoring of chemoprevention in a way that maximizes benefit and minimizes toxicity. ..
  36. Omega-3 Fatty Acids for Treatment of Major Depression
    David Mischoulon; Fiscal Year: 2010
    ..e. integrates biological findings with treatment in order to have a direct impact on the clinical practice of psychiatry. ..
  37. Epidemiologic Study of Placental Abruption
    Cande Ananth; Fiscal Year: 2006
    ..It will also add substantial new evidence for associations of several genetic thrombophilias and abruption risk. ..
  38. NSAID and COX/PG Metabolism and Colorectal Cancer
    Cornelia Ulrich; Fiscal Year: 2009
    ..Ultimately, such information on the metabolic variation will be useful in optimizing NSAIDs and NSAID regimens and will allow individual tailoring of chemoprevention. ..
  39. Vitamin B12 and Folate in Relation to Cognition in the Framingham Offspring Study
    Jacob Selhub; Fiscal Year: 2010
    ..5 About 3-5 percent of seniors have low circulating vitamin B12 levels;6 however, about 20 percent of seniors are found to be deficient when those with elevated methylmalonic acid levels are included. ..
  40. The Transcobalamin Receptor in Cobalamin Homeostasis
    Edward V Quadros; Fiscal Year: 2010
    ..abstract_text> ..
  41. GENE-NUTRIENT INTERACTIONS IN REDOX HOMEOSTASIS
    Ruma Banerjee; Fiscal Year: 2002
    ....
  42. FOLATE AND B12 IN NUTRITIONAL ANEMIAS--DIETARY CONTROL
    DONALD HORNE; Fiscal Year: 2002
    ....
  43. FOOD FOLATE FORTIFICATION EFFECT ON FOLATE STATUS
    Jacob Selhub; Fiscal Year: 2001
    ..abstract_text> ..
  44. POLYMORPHISMS IN PG/COX PATHWAY AND COLORECTAL POLYPS
    Cornelia Ulrich; Fiscal Year: 2003
    ..Finally, we plan to develop a model for integrating information on genetic variability at multiple points in this metabolic pathway. ..
  45. Measurement of folate in fortified cereal grain products
    Jacob Selhub; Fiscal Year: 2003
    ..In order to assess the impact of folic acid fortification, it is necessary to have valid data on the actual synthetic folic acid content of fortified foods. ..
  46. Inducible NOS as a Treatment Target in CLL
    J Weinberg; Fiscal Year: 2005
    ....
  47. Functional Arterial Changes in Atherogenesis
    Iftikhar Kullo; Fiscal Year: 2007
    ..abstract_text> ..
  48. Modeling Folate, One-Carbon Metabolism & DNA Methylation
    Cornelia Ulrich; Fiscal Year: 2007
    ..We plan to make the model generally available to the research community, and anticipate that it will become a useful tool that can be used as an adjunct to experimental investigations and as an aid to study design. ..
  49. Genetic Study of Prostaglandin Synthesis/EGFR and Risk of Colorectal Neoplasia
    Cornelia Ulrich; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  50. Gene-Nutrient Interactions and Breast Cancer
    SUSAN STECK; Fiscal Year: 2007
    ..abstract_text> ..
  51. Biomarkers of Peripheral Arterial Disease
    Iftikhar Kullo; Fiscal Year: 2007
    ..abstract_text> ..
  52. Exercise effects on oxidative damage among women
    Cornelia Ulrich; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  53. Proteomic Markers of Arteriosclerosis
    Iftikhar Kullo; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  54. Role of 10-formyldihydrofolate in purine biosynthesis
    Tsunenobu Tamura; Fiscal Year: 2004
    ..If this pathway is proven, derivatives of 10-HCO-H2folate could be used to inhibit de novo purine biosynthesis for developing new anticancer drugs. ..
  55. Gordon Research Sponsored Conference on Cobalamins
    Ruma Banerjee; Fiscal Year: 2003
    ....
  56. Determinants of arterial function in hypertension
    Iftikhar J Kullo; Fiscal Year: 2010
    ..The aim of our investigation is to identify novel proteins and genes that influence arterial function. Such work will help in identifying those at risk of developing arterial disease and facilitate development of new therapies. ..