nucleotidyltransferases

Summary

Summary: A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.

Top Publications

  1. ncbi Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain
    Y Masuda
    Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    J Biol Chem 276:15051-8. 2001
  2. pmc The Arabidopsis nucleotidyl transferase HESO1 uridylates unmethylated small RNAs to trigger their degradation
    Yuanyuan Zhao
    Department of Botany and Plant Sciences, Center for Plant Biology, Institute of Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA
    Curr Biol 22:689-94. 2012
  3. pmc Epigenetic instability due to defective replication of structured DNA
    Peter Sarkies
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 40:703-13. 2010
  4. pmc Structure of human cGAS reveals a conserved family of second-messenger enzymes in innate immunity
    Philip J Kranzusch
    Department of Molecular and Cell Biology, Center for RNA Systems Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Cell Rep 3:1362-8. 2013
  5. pmc DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity
    Noam Diamant
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Nucleic Acids Res 40:170-80. 2012
  6. pmc Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily
    Jane E Jackman
    Department of Biochemistry and Center for RNA Biology, The Ohio State University, Columbus, Ohio 43210, USA
    RNA 18:886-99. 2012
  7. ncbi Both UDP N-acetylglucosamine pyrophosphorylases of Tribolium castaneum are critical for molting, survival and fecundity
    Yasuyuki Arakane
    Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500 757, Korea
    Insect Biochem Mol Biol 41:42-50. 2011
  8. ncbi The Fanconi anaemia gene FANCC promotes homologous recombination and error-prone DNA repair
    Wojciech Niedzwiedz
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 15:607-20. 2004
  9. pmc Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover
    Olivia S Rissland
    Sir William Dunn School of Pathology, University of Oxford, UK
    Nat Struct Mol Biol 16:616-23. 2009
  10. pmc The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Polζ/Rev1-dependent translesion DNA synthesis
    Neeru M Sharma
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 286:33557-66. 2011

Detail Information

Publications253 found, 100 shown here

  1. ncbi Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain
    Y Masuda
    Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
    J Biol Chem 276:15051-8. 2001
    ..Therefore, the molecular mechanism of the dCMP transfer reaction of the REV1S protein and maybe also the REV1 protein might be the same as that of the dNTP transfer reaction of the translesion DNA polymerases...
  2. pmc The Arabidopsis nucleotidyl transferase HESO1 uridylates unmethylated small RNAs to trigger their degradation
    Yuanyuan Zhao
    Department of Botany and Plant Sciences, Center for Plant Biology, Institute of Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA
    Curr Biol 22:689-94. 2012
    ..We show that uridylation leads to miRNA degradation, and the degradation is most likely through an enzyme that is distinct from that causing the 3' truncation in hen1 mutants...
  3. pmc Epigenetic instability due to defective replication of structured DNA
    Peter Sarkies
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 40:703-13. 2010
    ....
  4. pmc Structure of human cGAS reveals a conserved family of second-messenger enzymes in innate immunity
    Philip J Kranzusch
    Department of Molecular and Cell Biology, Center for RNA Systems Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Cell Rep 3:1362-8. 2013
    ..These results demonstrate that cGAS and OAS define a family of innate immunity sensors and that structural divergence from a core nucleotidyltransferase enables second-messenger responses to distinct foreign nucleic acids...
  5. pmc DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity
    Noam Diamant
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Nucleic Acids Res 40:170-80. 2012
    ..Such a mechanism may function to maintain efficient replication, which can progress despite the presence of DNA lesions, with TLS lagging behind and patching regions of discontinuity...
  6. pmc Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily
    Jane E Jackman
    Department of Biochemistry and Center for RNA Biology, The Ohio State University, Columbus, Ohio 43210, USA
    RNA 18:886-99. 2012
    ..We also discuss possible evolutionary origins of Thg1 family-catalyzed 3'-to-5' addition activities and their implications for the currently observed phylogenetic distribution of Thg1-related enzymes in biology...
  7. ncbi Both UDP N-acetylglucosamine pyrophosphorylases of Tribolium castaneum are critical for molting, survival and fecundity
    Yasuyuki Arakane
    Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500 757, Korea
    Insect Biochem Mol Biol 41:42-50. 2011
    ..However, both of these genes appear to have additional critical role(s) unrelated to chitin synthesis, presumably in the glycosylation of proteins and/or secondary metabolites...
  8. ncbi The Fanconi anaemia gene FANCC promotes homologous recombination and error-prone DNA repair
    Wojciech Niedzwiedz
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 15:607-20. 2004
    ..These studies reveal that the FA proteins cooperate with key mutagenesis and repair processes that enable replication of damaged DNA...
  9. pmc Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover
    Olivia S Rissland
    Sir William Dunn School of Pathology, University of Oxford, UK
    Nat Struct Mol Biol 16:616-23. 2009
    ..As human cells contain Cid1 orthologs, uridylation may form the basis of a widespread, conserved mechanism of mRNA decay...
  10. pmc The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Polζ/Rev1-dependent translesion DNA synthesis
    Neeru M Sharma
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 286:33557-66. 2011
    ..The new mode of Rev1-PCNA binding described here suggests a mechanism by which Rev1 adopts a catalytically inactive configuration at the replication fork...
  11. ncbi A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage
    Adam Wood
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 282:20256-63. 2007
    ..Taken together, these studies suggest a specific mechanism by which the interaction between Rev1 and ubiquitinated PCNA is stabilized during the DNA damage response...
  12. pmc Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links
    J Kevin Hicks
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 2200, USA
    Mol Cell Biol 30:1217-30. 2010
    ....
  13. ncbi Interaction of hREV1 with three human Y-family DNA polymerases
    Eiji Ohashi
    Institute for Virus Research, Kyoto University, 53 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 9:523-31. 2004
    ..Thus, our results suggest that hREV1 plays a pivotal role in the multi-enzyme, multi-step process of translesion DNA synthesis...
  14. pmc Comprehensive classification of nucleotidyltransferase fold proteins: identification of novel families and their representatives in human
    Krzysztof Kuchta
    Laboratory of Bioinformatics and Bioengineering, Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 37:7701-14. 2009
    ..The determination of specific biological functions of these newly detected proteins remains a challenging task...
  15. pmc Sequential assembly of translesion DNA polymerases at UV-induced DNA damage sites
    Parker L Andersen
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, S7N 5E5, Canada
    Mol Biol Cell 22:2373-83. 2011
    ..These observations suggest a cooperative and sequential assembly of TLS polymerases in response to DNA damage. They also support and extend the current polymerase switch model...
  16. pmc The critical mutagenic translesion DNA polymerase Rev1 is highly expressed during G(2)/M phase rather than S phase
    Lauren S Waters
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:8971-6. 2006
    ....
  17. ncbi The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases
    Stewart Shuman
    Molecular Biology and Structural Biology Programs, Sloan Kettering Institute for Cancer Research, New York, New York 10021, USA
    Curr Opin Struct Biol 14:757-64. 2004
    ..The first crystal structure of an RNA ligase suggests that contemporary DNA ligases, RNA ligases and RNA capping enzymes evolved by fusion of ancillary effector domains to an ancestral catalytic module involved in RNA repair...
  18. pmc Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus
    C K Ho
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Mol Cell Biol 18:5189-98. 1998
    ..Hence, an RNA triphosphatase activity is essential for eukaryotic cell growth. This work highlights the potential for regulating mRNA cap formation through protein-protein interactions...
  19. pmc Uridylation of miRNAs by hen1 suppressor1 in Arabidopsis
    Guodong Ren
    Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska, Lincoln, NE 68588 0660, USA
    Curr Biol 22:695-700. 2012
    ..This study shall have implications on piRNA uridylation in hen1 in animals...
  20. ncbi Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism
    Daisuke Maruyama
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 282:17221-30. 2007
    ..However, a magnesium ion enhances the enzymatic activity of CaUAP1, and thus we propose that the magnesium ion increases the affinity between UTP and the enzyme by coordinating to the alpha- and gamma-phosphate group of UTP...
  21. pmc Separate domains of Rev1 mediate two modes of DNA damage bypass in mammalian cells
    Jacob G Jansen
    Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Biol 29:3113-23. 2009
    ..These gaps are inducers of DNA damage signaling leading to an irreversible G(2) arrest. Our results corroborate a model in which Rev1-mediated DNA damage bypass at postreplicative gaps quenches irreversible DNA damage responses...
  22. pmc tRNA(His) guanylyltransferase (THG1), a unique 3'-5' nucleotidyl transferase, shares unexpected structural homology with canonical 5'-3' DNA polymerases
    Samantha J Hyde
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    Proc Natl Acad Sci U S A 107:20305-10. 2010
    ..The ability of the same structural architecture to catalyze both 5'-3' and 3'-5' reactions raises important questions concerning selection of the 5'-3' mechanism during the evolution of nucleotide polymerases...
  23. ncbi Manipulation of L-ascorbic acid biosynthesis pathways in Solanum lycopersicum: elevated GDP-mannose pyrophosphorylase activity enhances L-ascorbate levels in red fruit
    Christelle Cronje
    Genetics Department, Institute for Plant Biotechnology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Planta 235:553-64. 2012
    ..In conclusion, the Smirnoff-Wheeler pathway showed great promise as a target for biotechnological manipulation of ascorbate levels in tomato...
  24. pmc The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site
    Keiji Hashimoto
    Department of Pharmacological Sciences, State University of New York, Stony Brook, New York 11794 8651, USA
    J Biol Chem 287:9613-22. 2012
    ..This indicates that a non-Y family polymerase(s) can insert a nucleotide opposite BPDE-dG, but the subsequent extension from miscoding termini depends on REV1-polζ in a RAD18-dependent manner...
  25. pmc Structure-dependent bypass of DNA interstrand crosslinks by translesion synthesis polymerases
    The Vinh Ho
    Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY 11794 3400, USA
    Nucleic Acids Res 39:7455-64. 2011
    ..Our study shows that ICLs can readily be bypassed by TLS polymerases if they are appropriately processed and that the structure of the ICL influences which polymerases are able to read through it...
  26. ncbi Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7
    Y Murakumo
    Department of Pathology, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Biol Chem 276:35644-51. 2001
    ..These findings suggest the possibility that hREV7 might play an important role in regulating the enzymatic activities of hREV1 and hREV3 for mutagenesis in response to DNA damage...
  27. ncbi Properties of GDP-mannose pyrophosphorylase, a critical enzyme and drug target in Leishmania mexicana
    Antony J Davis
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    J Biol Chem 279:12462-8. 2004
    ..Our studies provide insight into the structure of a novel target for the development of anti-Leishmania drugs...
  28. ncbi The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase II
    Silvia Jimeno-González
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus DK 8000, Denmark
    Mol Cell 37:580-7. 2010
    ..Our data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus...
  29. ncbi Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein
    Agnès Tissier
    UPR9OO3 CNRS, Cancerogenese et Mutagenese Moleculaire et Structurale, ESBS, Pole API Bd Sébastien Brant BP 10413, F 67412 Illkirch, France
    DNA Repair (Amst) 3:1503-14. 2004
    ..This hREV1 nuclear localization occurs independently of the presence of hpol eta. Taken together, our data suggest a central role for hREV1 as a scaffold that recruits DNA polymerases involved in TLS...
  30. pmc tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis
    Weifeng Gu
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    Genes Dev 17:2889-901. 2003
    ..Surprisingly, despite its varied activities, Thg1p contains no recognizable catalytic or functional domains...
  31. pmc tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases
    Jane E Jackman
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, New York 14642, USA
    RNA 12:1007-14. 2006
    ..Moreover, Thg1 and tRNA synthetases share two other features: They act in close proximity to one another at the top of the tRNA aminoacyl-acceptor stem, and the chemistry of their respective reactions is strikingly similar...
  32. pmc Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis
    Sean M Bulley
    Plant and Food Research, PB 92169, Auckland, New Zealand
    J Exp Bot 60:765-78. 2009
    ..These studies show the importance of GDP-L-galactose guanyltransferase as a rate-limiting step to AsA, and demonstrate how AsA can be significantly increased in plants...
  33. pmc Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature
    A P Bhavsar
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    J Bacteriol 183:6688-93. 2001
    ..Thickening of the wall and ultimately lysis followed these events...
  34. pmc Dual role of the molybdenum cofactor biosynthesis protein MOCS3 in tRNA thiolation and molybdenum cofactor biosynthesis in humans
    Mita Mullick Chowdhury
    Institute of Biochemistry and Biology, Department of Molecular Enzymology, University of Potsdam, Potsdam 14476, Germany
    J Biol Chem 287:17297-307. 2012
    ..The cellular localization results showed that extension of the C terminus with an additional glycine of MOCS2A and URM1 altered the localization of MOCS3 from the cytosol to the nucleus...
  35. ncbi Functional implications from the Cid1 poly(U) polymerase crystal structure
    Paola Munoz-Tello
    Department of Molecular Biology, University of Geneva, 30 Quai Ernest Ansermet, Geneva 1211, Switzerland
    Structure 20:977-86. 2012
    ..Our study underlines the Cid1 RNA binding properties, a feature with critical implications for miRNAs, histone mRNAs, and, more generally, cellular RNA degradation...
  36. ncbi Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase
    Luke A Yates
    Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Nat Struct Mol Biol 19:782-7. 2012
    ..Overall, our structures provide a basis for understanding the activity of Cid1 and a mechanism of UTP selectivity conserved in its human orthologs, suggesting potential implications for anticancer drug design...
  37. pmc Yeast Rev1 protein promotes complex formation of DNA polymerase zeta with Pol32 subunit of DNA polymerase delta
    Narottam Acharya
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1061, USA
    Proc Natl Acad Sci U S A 106:9631-6. 2009
    ..We discuss evidence for the biological significance of Rev1 binding to Pol32 for Pol zeta function in TLS and suggest a structural role for Rev1 in modulating the binding of Pol zeta with Pol32 in Pol delta stalled at a lesion site...
  38. ncbi The RNA capping machinery as an anti-infective target
    Moheshwarnath Issur
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Wiley Interdiscip Rev RNA 2:184-92. 2011
    ..Here, we examine various strategies that have been developed to inhibit microbial enzymes involved in the synthesis of the RNA cap structure, emphasizing the challenges remaining to design potent and selective drugs...
  39. ncbi Roles of the polymerase and BRCT domains of Rev1 protein in translesion DNA synthesis in yeast in vivo
    Chie Otsuka
    Department of Genomics and Proteomics, Okayama University Advanced Science Research Center, Tsushima, Okayama 700 8530, Japan
    Mutat Res 578:79-87. 2005
    ..The effect of Poleta was also analyzed. From all the results DNA polymerases other than these two translesion polymerases, too, seem to initiate the translesion synthesis...
  40. pmc Regulation of error-prone translesion synthesis by Spartan/C1orf124
    Myoung Shin Kim
    Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, MN 55905, USA
    Nucleic Acids Res 41:1661-8. 2013
    ..These results suggest that Spartan negatively regulates POLD3 function in Rev1/Pol ζ-dependent TLS, revealing a previously unrecognized regulatory step in error-prone TLS...
  41. pmc tRNA 5'-end repair activities of tRNAHis guanylyltransferase (Thg1)-like proteins from Bacteria and Archaea
    Bhalchandra S Rao
    Department of Biochemistry and Center for RNA Biology and Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH 43210, USA
    Nucleic Acids Res 39:1833-42. 2011
    ..These data support more widespread roles for 3'-5' nucleotide addition reactions in biology than previously expected...
  42. pmc A role for tRNA(His) guanylyltransferase (Thg1)-like proteins from Dictyostelium discoideum in mitochondrial 5'-tRNA editing
    Maria G Abad
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    RNA 17:613-23. 2011
    ..discoideum suggests that gene duplication and divergence during evolution has resulted in paralogous proteins that use 3'-5' nucleotide addition reactions for diverse biological functions in the same organism...
  43. pmc Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity
    Bradley M Lunde
    Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
    Nucleic Acids Res 40:9815-24. 2012
    ..By mutating this histidine to an asparagine residue in Cid1, we diminished Cid1's activity for UTP addition and improved ATP incorporation, supporting that this residue is important for UTP selectivity...
  44. pmc The relative roles in vivo of Saccharomyces cerevisiae Pol eta, Pol zeta, Rev1 protein and Pol32 in the bypass and mutation induction of an abasic site, T-T (6-4) photoadduct and T-T cis-syn cyclobutane dimer
    Peter E M Gibbs
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Genetics 169:575-82. 2005
    ..In contrast, bypass of the T-T dimer in the pol32delta strain occurs at the wild-type frequency...
  45. pmc Role of Dot1 in the response to alkylating DNA damage in Saccharomyces cerevisiae: regulation of DNA damage tolerance by the error-prone polymerases Polzeta/Rev1
    Francisco Conde
    Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Científicas and University of Salamanca, 37007 Salamanca, Spain
    Genetics 179:1197-210. 2008
    ..We propose that Dot1 inhibits translesion synthesis (TLS) by Polzeta/Rev1 and that the MMS resistance observed in the dot1 mutant results from the enhanced TLS activity...
  46. pmc Mutagenesis in eukaryotes dependent on DNA polymerase zeta and Rev1p
    C W Lawrence
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    Philos Trans R Soc Lond B Biol Sci 356:41-6. 2001
    ....
  47. pmc The DNA polymerase activity of Saccharomyces cerevisiae Rev1 is biologically significant
    Mary Ellen Wiltrout
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239, USA
    Genetics 187:21-35. 2011
    ..Furthermore, Pol ζ, but not Pol η, also contributes to 4-NQO resistance. These results show that Rev1's catalytic activity is important in vivo when the cell has to cope with specific DNA lesions, such as N(2)-dG...
  48. pmc Mutagenic effects of abasic and oxidized abasic lesions in Saccharomyces cerevisiae
    Yoke W Kow
    Department of Radiation Oncology, Emory University School of Medicine, 1365 B Clifton Road NE, Suite B5119, Atlanta, GA 30322, USA
    Nucleic Acids Res 33:6196-202. 2005
    ..In the rev1 and rev3 strains, dA was the predominant nucleotide inserted opposite these lesions. Overall, we conclude that both Rev1 and Polzeta are required for the efficient bypass of abasic sites in yeast...
  49. ncbi Towards Gram-negative antivirulence drugs: new inhibitors of HldE kinase
    Nicolas Desroy
    MUTABILIS SA, 102 Avenue Gaston Roussel, 93230 Romainville, France
    Bioorg Med Chem 17:1276-89. 2009
    ..Study of the structure-activity relationship of this series of inhibitors led to highly potent compounds...
  50. ncbi GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme
    I Pastuszak
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Biol Chem 273:30165-74. 1998
    ..The product of the reaction with GTP and alpha-L-fucose-1-P was characterized as GDP-beta-L-fucose by a variety of chemical and chromatographic methods...
  51. ncbi Functional characterization of GDP-mannose pyrophosphorylase from Leptospira interrogans serovar Copenhageni
    Matías D Asención Diez
    Laboratório de Bioquímica Microbiana, Universidad Nacional del Litoral, Santa Fe, Argentina
    Arch Microbiol 192:103-14. 2010
    ..Our studies provide insights into the structure of a novel molecular target, which could be useful for detection of leptospirosis and for the development of anti-leptospiral drugs...
  52. ncbi Identification of functional conserved residues of CTP:glycerol-3-phosphate cytidylyltransferase. Role of histidines in the conserved HXGH in catalysis
    Y S Park
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 272:15161-6. 1997
    ..e. to stabilize a pentacoordinate transition state...
  53. pmc Pre-steady state kinetic studies of the fidelity of nucleotide incorporation by yeast DNA polymerase delta
    Lynne M Dieckman
    Department of Biochemistry, University of Iowa College of Medicine, Iowa City, Iowa 52242 1109, USA
    Biochemistry 49:7344-50. 2010
    ..We suggest that, unlike its prokaryotic counterparts, pol delta requires replication accessory factors like proliferating cell nuclear antigen to achieve rapid rates of nucleotide incorporation...
  54. ncbi Negative cooperativity of substrate binding but not enzyme activity in wild-type and mutant forms of CTP:glycerol-3-phosphate cytidylyltransferase
    S Sanker
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0606, USA
    J Biol Chem 276:37922-8. 2001
    ..These data support a mechanism in which catalysis occurs only when the enzyme is fully loaded with 2 molecules of each substrate/enzyme dimer...
  55. ncbi ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand break
    Yukinori Hirano
    Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07103, USA
    Curr Biol 16:586-90. 2006
    ..Our results reveal a novel role of Mec1 in the localization of the Polzeta-Rev1 complex to DNA lesions and highlight a linkage of TLS polymerases to the checkpoint response...
  56. ncbi DNA polymerase C of the thermophilic bacterium Thermus aquaticus: classification and phylogenetic analysis of the family C DNA polymerases
    Y P Huang
    Department of Microbiology and Immunology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA
    J Mol Evol 48:756-69. 1999
    ..Third, the cyanobacterial family C DNA pol, classified as class III because it is encoded by a split gene, forms a group with the high-G+C Gram-positive bacteria...
  57. pmc The flavivirus NS5 protein is a true RNA guanylyltransferase that catalyzes a two-step reaction to form the RNA cap structure
    Moheshwarnath Issur
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    RNA 15:2340-50. 2009
    ..Our study provides biochemical evidence that flaviviruses encode a complete RNA capping machinery...
  58. pmc Kinetic analysis of 3'-5' nucleotide addition catalyzed by eukaryotic tRNA(His) guanylyltransferase
    Brian A Smith
    Department of Biochemistry, Center for RNA Biology and Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, United States
    Biochemistry 51:453-65. 2012
    ..These data provide a foundation for understanding the mechanism of 3'-5' nucleotide addition in tRNA(His) maturation...
  59. ncbi The broad spectrum antiviral nucleoside ribavirin as a substrate for a viral RNA capping enzyme
    Isabelle Bougie
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    J Biol Chem 279:22124-30. 2004
    ..The biological implications of these findings for the proposed ribavirin-mediated inhibition of capping are discussed...
  60. ncbi Kinetic and thermodynamic characterization of the RNA guanylyltransferase reaction
    Marie F Soulière
    RNA Group Grpe ARN, Departement de Biochimie, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Biochemistry 47:3863-74. 2008
    ..This study reports the first thorough kinetic and thermodynamic characterization of the reaction catalyzed by an RNA capping enzyme...
  61. pmc Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae
    M Wu
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Mol Cell Biol 15:264-71. 1995
    ..This mechanism of suppression is supported by the presence of mitochondrial FAD synthetase activity in S. cerevisiae transformed with FAD1 on a high-copy-number plasmid but not in mitochondrial of a wild-type strain...
  62. ncbi UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts
    Toshihisa Kotake
    Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo okubo, Sakura ku, Saitama 338 8570, Japan
    J Biol Chem 279:45728-36. 2004
    ..These results indicate that the enzyme is a novel type of UDP-sugar pyrophosphorylase, which catalyzes the formation of various UDP-sugars at the end of salvage pathways in higher plants...
  63. pmc Mutational specificity and genetic control of replicative bypass of an abasic site in yeast
    Vincent Pages
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1061, USA
    Proc Natl Acad Sci U S A 105:1170-5. 2008
    ....
  64. pmc The synthesis of UDP-N-acetylglucosamine is essential for bloodstream form trypanosoma brucei in vitro and in vivo and UDP-N-acetylglucosamine starvation reveals a hierarchy in parasite protein glycosylation
    Matthew J Stokes
    Division of Biological Chemistry and Drug Discovery, The Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 283:16147-61. 2008
    ..The significance of this finding, with respect to the hierarchy of site-specific N-glycosylation in T. brucei, is discussed...
  65. pmc Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain
    Phi Luong
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 285:20155-63. 2010
    ..Discovery of a ternary reaction mechanism along with structural insight provides critical groundwork for future studies for the family of AMPylators that modify hydroxyl-containing residues with AMP...
  66. pmc Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1
    Kodai Hara
    Graduate School of Nanobioscience, Yokohama City University, Tsurumi ku, Yokohama 230 0045, Japan
    J Biol Chem 285:12299-307. 2010
    ..Our results will provide a general structural basis for understanding the REV7 interaction...
  67. ncbi SHAPE analysis of long-range interactions reveals extensive and thermodynamically preferred misfolding in a fragile group I intron RNA
    Caia D S Duncan
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 47:8504-13. 2008
    ....
  68. pmc The requirement for the highly conserved G-1 residue of Saccharomyces cerevisiae tRNAHis can be circumvented by overexpression of tRNAHis and its synthetase
    Melanie A Preston
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA
    RNA 16:1068-77. 2010
    ....
  69. pmc Template-dependent 3'-5' nucleotide addition is a shared feature of tRNAHis guanylyltransferase enzymes from multiple domains of life
    Maria G Abad
    Department of Biochemistry and Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 107:674-9. 2010
    ....
  70. pmc A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron
    M Matsuura
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210 1292, USA
    Genes Dev 11:2910-24. 1997
    ..Expression in E. coli facilitates the use of group II introns for the targeting of specific foreign sequences to a desired site in DNA...
  71. pmc Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC)
    G Johnson
    Division of Microbiology, Hospital for Sick Children, University of Toronto Cabbagetown Pet Clinic, Toronto, Ontario, Canada
    J Clin Microbiol 41:1069-72. 2003
    ..berkhoffii. Species identification is achieved simply by restriction enzyme digestion of the amplicon. This PCR assay appears to be specific for the Bartonella genus because it failed to amplify DNA from several other bacterial species...
  72. ncbi UDP-sugar pyrophosphorylase is essential for pollen development in Arabidopsis
    Judy A Schnurr
    Plant Science Research, United States Department of Agriculture Agricultural Research Services, St Paul, MN 55108, USA
    Planta 224:520-32. 2006
    ..Evidence presented here shows that AtUSP has a critical role in pollen development...
  73. pmc Enzymology of RNA cap synthesis
    Agnidipta Ghosh
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Wiley Interdiscip Rev RNA 1:152-72. 2010
    ..Although mRNA capping is conserved among viruses and eukaryotes, some viruses have adopted strategies for capping mRNA that are distinct from the cellular mRNA capping pathway...
  74. ncbi Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function
    Satya Prakash
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1061, USA
    Annu Rev Biochem 74:317-53. 2005
    ....
  75. pmc Thermodynamics of ligand binding by the yeast mRNA-capping enzyme reveals different modes of binding
    Isabelle Bougie
    Departement de Biochimie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4
    Biochem J 384:411-20. 2004
    ..In addition to the determination of the energetics of ligand binding, our study leads to a better understanding of the molecular basis of substrate recognition by RNA-capping enzymes...
  76. ncbi Purification and characterization of phosphopantetheine adenylyltransferase from Escherichia coli
    A Geerlof
    Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 274:27105-11. 1999
    ..Since purified PPAT lacks dephospho-CoA kinase activity, the two final steps of CoA biosynthesis in E. coli must be catalyzed by separate enzymes...
  77. pmc Structure of the human Rev1-DNA-dNTP ternary complex
    Michael K Swan
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Mol Biol 390:699-709. 2009
    ....
  78. ncbi Identification of a novel UDP-sugar pyrophosphorylase with a broad substrate specificity in Trypanosoma cruzi
    Ting Yang
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, 30602, USA
    Biochem J 429:533-43. 2010
    ....
  79. pmc 3'-5' tRNAHis guanylyltransferase in bacteria
    Ilka U Heinemann
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    FEBS Lett 584:3567-72. 2010
    ..Complementation of a yeast thg1 knockout strain with bacterial Thg1 verified in vivo activity and suggests a relaxed recognition of the discriminator base in bacteria...
  80. pmc Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis
    P L Conklin
    Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Ithaca, NY 14853 1801, USA
    Proc Natl Acad Sci U S A 96:4198-203. 1999
    ....
  81. ncbi Quantitative trait locus analysis, pathway analysis, and consomic mapping show genetic variants of Tnni3k, Fpgt, or H28 control susceptibility to viral myocarditis
    Sean A Wiltshire
    Department of Human Genetics, McGill University, Montreal, Quebec H3A 1B1, Canada
    J Immunol 186:6398-405. 2011
    ..Differentially expressed pathways were identified by microarray, and candidate gene analysis revealed Fpgt, H28, and Tnni3k as likely candidates for Vms1...
  82. ncbi Trypanosoma brucei encodes a bifunctional capping enzyme essential for cap 4 formation on the spliced leader RNA
    Yuko Takagi
    Department of Biological Sciences, State University of New York, Buffalo, New York 14260, USA
    J Biol Chem 282:15995-6005. 2007
    ..We conclude that TbCgm1 specifically cap SL RNA, and cap 0 is a prerequisite for subsequent methylation events leading to the formation of mature SL RNA...
  83. pmc Presence of a classical RRM-fold palm domain in Thg1-type 3'- 5'nucleic acid polymerases and the origin of the GGDEF and CRISPR polymerase domains
    Vivek Anantharaman
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Biol Direct 5:43. 2010
    ....
  84. pmc Inhibitors of Leishmania GDP-mannose pyrophosphorylase identified by high-throughput screening of small-molecule chemical library
    Kurt Lackovic
    High Throughput Chemical Screening Facility, Structural Biology Division, Walter Eliza Hall Institute of Medical Research, Melbourne, Australia
    Antimicrob Agents Chemother 54:1712-9. 2010
    ..We have identified novel candidate compounds for the design and synthesis of antileishmanial therapeutics...
  85. pmc Plant mitochondria use two pathways for the biogenesis of tRNAHis
    Antonio Placido
    Dipartimento di Biochimica e Biologia Molecolare Ernesto Quagliariello, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy
    Nucleic Acids Res 38:7711-7. 2010
    ..This shows that a previously unreported tRNA(His) guanylyltransferase activity is present in plant mitochondria...
  86. pmc Structural analysis of FAD synthetase from Corynebacterium ammoniagenes
    Susana Frago
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias and Institute of Biocomputation and Physics of Complex Systems, Universidad de Zaragoza, Zaragoza, Spain
    BMC Microbiol 8:160. 2008
    ..The prokaryotic FAD synthetase family - a group of bifunctional enzymes that catalyse riboflavin phosphorylation and FMN adenylylation within a single polypeptide chain- was analysed in terms of sequence and structure...
  87. pmc tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition
    Jane E Jackman
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 103:8640-5. 2006
    ..These results suggest that 3'-5' polymerization represents either an uncharacterized role for Thg1 in RNA or DNA repair or metabolism, or it may be a remnant of an earlier catalytic strategy used in nature...
  88. pmc Fido, a novel AMPylation domain common to fic, doc, and AvrB
    Lisa N Kinch
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS ONE 4:e5818. 2009
    ..The VopS fic domain includes a conserved sequence motif (HPFx[D/E]GN[G/K]R) that contributes to AMPylation. Fic domains are found in a variety of species, including bacteria, a few archaea, and metazoan eukaryotes...
  89. ncbi The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status
    Teresa A Giancaspero
    Dipartimento di Biochimica e Biologia Molecolare E Quagliariello, Universita degli Studi di Bari, Bari, Italy
    FEBS J 276:219-31. 2009
    ..1, respectively). The FAD-forming enzymes are both activated by MgCl(2), and reside in two distinct monofunctional enzymes, which can be physically separated in mitochondrial soluble and membrane-enriched fractions, respectively...
  90. ncbi DNA polymerase zeta (pol zeta) in higher eukaryotes
    Gregory N Gan
    Department of Pharmacology, University of Pittsburgh Medical School, Pittsburgh, PA 15213, USA
    Cell Res 18:174-83. 2008
    ..This review discusses recent developments in these areas...
  91. pmc Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron
    Michal T Boniecki
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 284:26243-50. 2009
    ..The activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS...
  92. ncbi CTP:glycerol 3-phosphate cytidylyltransferase (TarD) from Staphylococcus aureus catalyzes the cytidylyl transfer via an ordered Bi-Bi reaction mechanism with micromolar K(m) values
    David S Badurina
    Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Room 4H2, 1200 Main St West, Hamilton, Ontario, Canada L8N 3Z5
    Biochim Biophys Acta 1646:196-206. 2003
    ..subtilis 168 mutant defective in tagD (temperature-sensitive, tag-12). Growth of the resulting strain at the restrictive temperature (47 degrees C) was shown to be xylose-dependent...
  93. pmc Poleta, Polzeta and Rev1 together are required for G to T transversion mutations induced by the (+)- and (-)-trans-anti-BPDE-N2-dG DNA adducts in yeast cells
    Bo Zhao
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 34:417-25. 2006
    ..Based on biochemical and genetic results, we present mechanistic models of translesion synthesis of these two DNA adducts, involving both the one-polymerase one-step and two-polymerase two-step models...
  94. pmc The Werner's Syndrome protein collaborates with REV1 to promote replication fork progression on damaged DNA
    Lara G Phillips
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    DNA Repair (Amst) 9:1064-72. 2010
    ....
  95. ncbi Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli
    C P Sparrow
    J Biol Chem 260:12084-91. 1985
    ..The substrate specificity of the enzyme is also investigated. This is the first time this enzyme has been purified to homogeneity from any source, despite the fact that it is essential for phospholipid biosynthesis in all organisms...
  96. ncbi Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis
    S B Richard
    Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Biol 8:641-8. 2001
    ..Moreover, these complexes represent the first experimental structures for any cytidyltransferase with both substrates and products bound...
  97. pmc The human REV1 gene codes for a DNA template-dependent dCMP transferase
    W Lin
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
    Nucleic Acids Res 27:4468-75. 1999
    ..Consistent with its role as a fundamental mutagenic protein, the REV1 gene is ubiquitously expressed in various human tissues...
  98. ncbi Kinetic analysis of Escherichia coli 2-C-methyl-D-erythritol-4-phosphate cytidyltransferase, wild type and mutants, reveals roles of active site amino acids
    Stéphane B Richard
    Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 43:12189-97. 2004
    ..In addition, Thr140, Arg109, Asp106, and Thr165 were all shown to play critical roles in the binding and proper orientation of the MEP substrate...
  99. pmc Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory
    Hoo Sun Chung
    Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
    Plant Physiol 146:952-64. 2008
    ..Our results also indicate that the timing of JA-induced transcription in response to wounding is more rapid than previously realized...
  100. pmc Kinetic basis of nucleotide selection employed by a protein template-dependent DNA polymerase
    Jessica A Brown
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 49:5504-10. 2010
    ....
  101. ncbi A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity
    Cristobal Bernal
    Departament de Bioquimica i Biologia Molecular, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain
    Anal Biochem 337:55-61. 2005
    ..The method is easy to perform, sensitive, and robust and can be used in automated high-throughput screening analyses for the search of inhibitors of the enzyme...

Research Grants26

  1. SYNTHETIC SUBSTRATES FOR NUCLEOTIDYLTRANSFERASE
    DAVID THURLOW; Fiscal Year: 1991
    ..These RNAs will be used, together with nucleotidyltransferases from bacterial, fungal and mammalian sources, in nitrocellulose filter binding assays to determine ..
  2. Mechanisms of DNA Lesion Bypass in Eukaryotes
    Zhigang Wang; Fiscal Year: 2006
    ..The proposed studies should significantly advance our understanding of mechanisms of lesion bypass and damage-induced mutagenesis in eukaryotic cells. ..
  3. MOLECULAR DISSECTION OF YEAST NUCLEOTIDE EXCISION REPAIR
    Zhigang Wang; Fiscal Year: 2006
    ..These studies should lead to a better understanding of the NER pathway in eukaryotes, which in turn should advance our understanding on the interaction between human health and many genotoxic agents in our environment. ..
  4. Transcriptional Nitrogen Regulation in Methanococcus
    JOHN LEIGH; Fiscal Year: 2007
    ..These studies in Archaea have wide importance, as regulatory mechanisms are central to human development and disease. ..
  5. C-C & C-N Bond Formation in Unusual Sugar Biosynthesis
    Hung wen Liu; Fiscal Year: 2007
    ..Overall, our results are expected to make a significant contribution to the fundamentals of enzyme chemistry and possibly impact pharmaceutical biotechnology. ..
  6. Incident HIV Infection and Immune Response to Hepatitis C Virus
    Andrea Cox; Fiscal Year: 2007
    ..This, along with excellent mentorship and the supportive and rich environment at JHU, will provide Dr. Cox with the skills she needs to develop into an independent researcher studying HIV/HCV coinfection immunology. ..
  7. MECHANISMS OF BIOSYNTHETIC FORMATION OF DEOXY SUGARS
    Hung wen Liu; Fiscal Year: 2008
    ..Therefore, our anticipated results should make a significant contribution to the broad field of bioorganic mechanistic enzymology and have possible impact on pharmaceutical biotechnology. ..
  8. Exocyclic C-C Modifications of Aromatic Polyketides
    JON THORSON; Fiscal Year: 2008
    ..A particular focus of the final diversification strategies presented will be focused upon conjugation of these highly reactive antitumor antibiotics with known tumor-directing peptides. ..
  9. Transcription-coupled DNA repair in yeast
    Satya Prakash; Fiscal Year: 2008
    ..abstract_text> ..
  10. Repair of Clustered DNA Damages
    YOKE KOW; Fiscal Year: 2008
    ..Completion of these three aims is expected to provide significant understanding on how cells process tandem lesions and the biological consequences of incompletely repaired tandem lesions. ..
  11. Repair of Clustered DNA Damages
    Yoke W Kow; Fiscal Year: 2010
    ..In addition, it will provide significant insight as to the potential mechanism involved in melanoma progression. ..
  12. Hoogsteen base pairing in human DNA polymerase iota
    Satya Prakash; Fiscal Year: 2010
    ..abstract_text> ..
  13. MECHANISTIC STUDIES OF PROKARYOTIC MANNOSYLTRANSFERASES
    JON THORSON; Fiscal Year: 2002
    ....
  14. ENZYME MEDIATED THIOL MODIFICATION OF TRNA
    CHARLES LAUHON; Fiscal Year: 2002
    ....
  15. DNA MISMATCH REPAIR IN EUKARYOTES
    Satya Prakash; Fiscal Year: 2003
    ..Because of the remarkable conservation of the mismatch repair machinery between yeast and humans, these studies should provide important and novel insights into the mechanisms of this repair process in humans. ..
  16. DNA SEQUENCING OF UV INDUCED CHROMOSOMAL MUTATIONS
    Christopher Lawrence; Fiscal Year: 2003
    ..abstract_text> ..
  17. MECHANISM OF TRANSLESION REPLICATION IN EUKARYOTES
    Christopher Lawrence; Fiscal Year: 2003
    ..These plasmids have proved useful in detecting different kinds of recombinant events, a likely mechanism for the pathway. ..
  18. Viral DNA Replication and Late Transcription
    Linda Guarino; Fiscal Year: 2004
    ..Knowledge gained from these experiments should be relevant to other viral systems and will provide a better understanding of the complex interactions between transcription and replication machinery in eukaryotic cells. ..
  19. Crystallographic Analysis of NMN Adenylytransferases
    Hong Zhang; Fiscal Year: 2005
    ..influenzae, and the crystallization trials are in progress. Elucidating the structures of NadR in its various ligand-bound forms and its complex with DNA will reveal how its conformation and function are modulated by both NAD and ATP. ..
  20. Crystallographic Analysis of GHMP kinases
    Hong Zhang; Fiscal Year: 2005
    ....
  21. The Mammalian Signal Recognition Particle
    KEVIN WEEKS; Fiscal Year: 2006
    ..Moreover, the work is designed to identify principles generalizable to other biologically and medically prominent ribonucleoprotein complexes. ..
  22. Protein-Facilitates RNA Catalysts
    KEVIN WEEKS; Fiscal Year: 2007
    ..5) To begin to explore the molecular mechanism by which Mrs1 facilitates catalysis of the bl3 group I intron. (6) To develop a high resolution model for the maturase-bl3 RNA complex. ..
  23. ENDOTHELIAL & KERATINOCYTE STEM CELLS IN WOUND HEALING
    Gina Schatteman; Fiscal Year: 2004
    ..We will also investigate whether diabetes affects the incorporation of these labeled cells into different skin components. ..
  24. GENOME SEQUENCE AND REGULATION IN M MARIPALUDIS
    JOHN LEIGH; Fiscal Year: 2002
    ..Genes will be identified that either vary in expression with changing conditions, or change in expression after mutagenesis of regulatory genes. ..