dna nucleotidylexotransferase

Summary

Summary: A non-template-directed DNA polymerase normally found in vertebrate thymus and bone marrow. It catalyzes the elongation of oligo- or polydeoxynucleotide chains and is widely used as a tool in the differential diagnosis of acute leukemias in man. EC 2.7.7.31.

Top Publications

  1. pmc Polymerase mu is a DNA-directed DNA/RNA polymerase
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:2309-15. 2003
  2. pmc The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization
    L A Bentolila
    Unité de Génétique et Biochimie du Développement, Paris Cedex 15, France
    EMBO J 14:4221-9. 1995
  3. pmc A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis
    Raquel Juarez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Campus Universidad Autónoma Canto Blanco, 28049 Madrid, Spain
    Nucleic Acids Res 34:4572-82. 2006
  4. ncbi A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell 19:357-66. 2005
  5. ncbi Characterization of rainbow trout terminal deoxynucleotidyl transferase structure and expression. TdT and RAG1 co-expression define the trout primary lymphoid tissues
    J D Hansen
    The Basel Institute for Immunology, Grenzacherstrasse 487, CH 4005 Basel, Switzerland
    Immunogenetics 46:367-75. 1997
  6. pmc Distinct requirements for Ku in N nucleotide addition at V(D)J- and non-V(D)J-generated double-strand breaks
    Zoltan Sandor
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 32:1866-73. 2004
  7. ncbi V(D)J recombination in Ku86-deficient mice: distinct effects on coding, signal, and hybrid joint formation
    M A Bogue
    Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Immunity 7:37-47. 1997
  8. pmc DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells
    O Dominguez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Centro Nacional de Biotecnologia CSIC, Universidad Autonoma, 28049 Madrid, Spain
    EMBO J 19:1731-42. 2000
  9. pmc Incorporation of non-nucleoside triphosphate analogues opposite to an abasic site by human DNA polymerases beta and lambda
    Emmanuele Crespan
    Istituto di Genetica Molecolare IGM CNR via Abbiategrasso 207, I 27100 Pavia, Italy
    Nucleic Acids Res 33:4117-27. 2005
  10. ncbi Bood POZ containing gene type 2 is a human counterpart of yeast Btb3p and promotes the degradation of terminal deoxynucleotidyltransferase
    So Maezawa
    Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Genes Cells 13:439-57. 2008

Detail Information

Publications224 found, 100 shown here

  1. pmc Polymerase mu is a DNA-directed DNA/RNA polymerase
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:2309-15. 2003
    ....
  2. pmc The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization
    L A Bentolila
    Unité de Génétique et Biochimie du Développement, Paris Cedex 15, France
    EMBO J 14:4221-9. 1995
    ..All these characteristics could contribute to the functional difference between the two isoforms of TdT. However, the subcellular localization of TdTL on its own can account for its inability to add N regions...
  3. pmc A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis
    Raquel Juarez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Campus Universidad Autónoma Canto Blanco, 28049 Madrid, Spain
    Nucleic Acids Res 34:4572-82. 2006
    ..Recent in vivo and in vitro data suggest that such a dual capacity could be advantageous to resolve microhomology-mediated end-joining reactions...
  4. ncbi A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell 19:357-66. 2005
    ..This apparent gradient of template dependence is largely attributable to a small structural element that is present but different in all three polymerases...
  5. ncbi Characterization of rainbow trout terminal deoxynucleotidyl transferase structure and expression. TdT and RAG1 co-expression define the trout primary lymphoid tissues
    J D Hansen
    The Basel Institute for Immunology, Grenzacherstrasse 487, CH 4005 Basel, Switzerland
    Immunogenetics 46:367-75. 1997
    ..Finally, TdT cDNA was amplified from embryos at 20 days post-fertilization (pf), which correlates with the appearence of the thymus and pronephros anlage during trout ontogeny...
  6. pmc Distinct requirements for Ku in N nucleotide addition at V(D)J- and non-V(D)J-generated double-strand breaks
    Zoltan Sandor
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 32:1866-73. 2004
    ..We also found that N regions were abnormally long in the absence of Ku80, indicating that Ku80 may regulate TdT's activity at DNA ends in vivo...
  7. ncbi V(D)J recombination in Ku86-deficient mice: distinct effects on coding, signal, and hybrid joint formation
    M A Bogue
    Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Immunity 7:37-47. 1997
    ..Furthermore, Ku86(-/-) joints are unexpectedly devoid of N-region diversity, suggesting a novel role for Ku in the addition of N nucleotides by terminal deoxynucleotidyl transferase...
  8. pmc DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells
    O Dominguez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Centro Nacional de Biotecnologia CSIC, Universidad Autonoma, 28049 Madrid, Spain
    EMBO J 19:1731-42. 2000
    ..Therefore, Pol mu is a good candidate to be the mutator polymerase responsible for somatic hyper- mutation of immunoglobulin genes...
  9. pmc Incorporation of non-nucleoside triphosphate analogues opposite to an abasic site by human DNA polymerases beta and lambda
    Emmanuele Crespan
    Istituto di Genetica Molecolare IGM CNR via Abbiategrasso 207, I 27100 Pavia, Italy
    Nucleic Acids Res 33:4117-27. 2005
    ..These results show for the first time that neither the base nor the sugar moieties of nucleotides are required for incorporation by family X DNA polymerases...
  10. ncbi Bood POZ containing gene type 2 is a human counterpart of yeast Btb3p and promotes the degradation of terminal deoxynucleotidyltransferase
    So Maezawa
    Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Genes Cells 13:439-57. 2008
    ..TdT was ubiquitinated only within the nucleus and degraded by the 26S proteasome. The ubiqutination or degradation of TdT was markedly promoted by co-expression of BPOZ-2 and CUL3 or BPOZ-2 in 293T cells, respectively...
  11. ncbi Constitutive expression of terminal deoxynucleotidyl transferase in transgenic mice is sufficient for N region diversity to occur at any Ig locus throughout B cell differentiation
    L A Bentolila
    Unit of Genetics and Developmental Biochemistry, National Center for Scientific Research, Paris, France
    J Immunol 158:715-23. 1997
    ..Constitutive expression of TdT throughout B cell differentiation does not therefore appear deleterious and suggests that TdT is recruited only to participate in the V(D)J recombination process...
  12. pmc Ku80 is required for addition of N nucleotides to V(D)J recombination junctions by terminal deoxynucleotidyl transferase
    M M Purugganan
    Department of Immunology, M929, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Nucleic Acids Res 29:1638-46. 2001
    ..Based on these data, we propose that TdT does not add to recombination junctions through random collision but is actively recruited to the V(D)J recombinase complex by Ku80...
  13. ncbi Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes
    T Komori
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115
    Science 261:1171-5. 1993
    ....
  14. pmc Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase
    M Delarue
    Unité de Biochimie Structurale, URA 2185 du CNRS, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris France
    EMBO J 21:427-39. 2002
    ..The present structure can be used to model the recently discovered human polymerase mu, with which it shares 43% sequence identity...
  15. pmc DNA polymerase mu, a candidate hypermutase?
    J F Ruiz
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma, Madrid, Spain
    Philos Trans R Soc Lond B Biol Sci 356:99-109. 2001
    ....
  16. pmc Association of terminal deoxynucleotidyl transferase with Ku
    K N Mahajan
    University of North Carolina Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 96:13926-31. 1999
    ..The physical association of TdT with Ku suggests a possible mechanism by which TdT is recruited to the sites of DSBs such as V(D)J recombination intermediates...
  17. pmc The CDR-H3 repertoire from TdT-deficient adult bone marrow is a close, but not exact, homologue of the CDR-H3 repertoire from perinatal liver
    Robert L Schelonka
    Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Immunol 185:6075-84. 2010
    ..Thus, although differing in V(H) content, TdT-deficient mice appear to represent a good, although not perfect, model for testing the role of perinatal CDR-H3 limitations on late B cell development and Ab responses...
  18. ncbi Terminal deoxynucleotidyltransferase deficiency decreases autoimmune disease in diabetes-prone nonobese diabetic mice and lupus-prone MRL-Fas(lpr) mice
    Ian F Robey
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 172:4624-9. 2004
    ..We suggest that the paucity of autoreactive specificities created in the N region-lacking repertoire, and the resultant protection afforded to the newborn, may be the reason that TdT expression is delayed in ontogeny...
  19. ncbi Terminal deoxynucleotidyl transferase is required for an optimal response to the polysaccharide α-1,3 dextran
    Tamer I Mahmoud
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Immunol 184:851-8. 2010
    ..These data show that TdT is essential for the generation of the predominant higher-affinity DEX-responsive J558 clone...
  20. ncbi Enzymatic synthesis of fluorescent oligomers assembled on a DNA backbone
    Younjin Cho
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Chembiochem 7:669-72. 2006
    ..The enzymatic synthesis of the pyrene excimer might have applications in homogeneous biological assays for DNA fragments, such as those that arise during apoptosis...
  21. ncbi Distinct and opposite diversifying activities of terminal transferase splice variants
    To Ha Thai
    Division of Developmental and Clinical Immunology, Department of Microbiology, The University of Alabama at Birmingham, 35294, USA
    Nat Immunol 3:457-62. 2002
    ..These findings suggest that the two TdT isoforms may act in concert to preserve the integrity of the variable region of antigen receptors while generating diversity...
  22. ncbi The M603 idiotype is lost in the response to phosphocholine in terminal deoxynucleotidyl transferase-deficient mice
    Qing Sheng Mi
    Laboratory of Immunology, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore 21224, USA
    Eur J Immunol 32:1139-46. 2002
    ..These data indicate that production of M603-id antibody is TdT dependent, while generation of M167-id antibody is TdT independent, and that in the absence of competition from M603-like B cells, T15-id B cells can respond to PC-PM...
  23. pmc Pyrophosphorolytic dismutation of oligodeoxy-nucleotides by terminal deoxynucleotidyltransferase
    R S Anderson
    Baylor College of Medicine, Department of Biochemistry, One Baylor Plaza, Houston TX 77030, USA
    Nucleic Acids Res 27:3190-6. 1999
    ..These results may help explain the GC-richness of immunoglobulin gene segment joins (N regions) and the loss of bases that occur during gene rearrangements in pre-B and pre-T cells...
  24. ncbi Terminal deoxynucleotidyl transferase deficiency reduces the incidence of autoimmune nephritis in (New Zealand Black x New Zealand White)F1 mice
    C Conde
    Laboratory of Immunopathology, Institute of Immuno Hematology, Central Hospital, Strasbourg, France
    J Immunol 161:7023-30. 1998
    ..Moreover, our results may help to unravel the mechanisms of lupus nephritis...
  25. ncbi Indolent T-lymphoblastic proliferation: report of a case with an 11-year history and association with myasthenia gravis
    J A Strauchen
    Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Am J Surg Pathol 25:411-5. 2001
    ..Indolent T-lymphoblastic proliferations of undetermined clonality may rarely occur; predilection for involvement of oropharynx and possible association with myasthenia gravis are suggested...
  26. ncbi Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo
    Barbara Bertocci
    INSERM U783, Faculté de Médecine René Descartes, Site Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
    Immunity 25:31-41. 2006
    ....
  27. ncbi Human DNA polymerase lambda possesses terminal deoxyribonucleotidyl transferase activity and can elongate RNA primers: implications for novel functions
    Kristijan Ramadan
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 328:63-72. 2003
    ..These two novel properties of human DNA polymerase lambda might suggest additional roles for this enzyme in DNA replication and repair processes...
  28. ncbi B-1 cells: the lineage question revisited
    L A Herzenberg
    Department of Genetics, Stanford University School of Medicine, CA 94305, USA
    Immunol Rev 175:9-22. 2000
    ....
  29. ncbi Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides
    J B Boulé
    Unité de Génétique et Biochimie du Développement, URA CNRS 1960, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    J Biol Chem 276:31388-93. 2001
    ..An essential catalytic aspartate in TdT was identified, which is a first step toward understanding the apparent lack of sugar discrimination by TdT...
  30. pmc Characterization of SpPol4, a unique X-family DNA polymerase in Schizosaccharomyces pombe
    Sergio González-Barrera
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma, Madrid, Spain
    Nucleic Acids Res 33:4762-74. 2005
    ..Therefore, SpPol4 is a unique enzyme whose enzymatic properties are hybrid of those described for mammalian Polbeta, Pollambda and Polmu...
  31. ncbi Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase
    B Yang
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill 27599 7260
    J Biol Chem 269:11859-68. 1994
    ..These results indicated that the aspartic acid residue at position 343 is located at or near the active site and is critical for the nucleotide binding and catalytic activity...
  32. ncbi Terminal deoxynucleotidyl transferase deficiency decreases autoimmune disease in MRL-Fas(lpr) mice
    A J Feeney
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 167:3486-93. 2001
    ..Hence, either the T or B cell repertoires, or more likely both, require N region diversity to produce the full spectrum of autoimmune lupus disease...
  33. ncbi Sibling rivalry: competition between Pol X family members in V(D)J recombination and general double strand break repair
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Immunol Rev 200:156-64. 2004
    ..Recent work implicates several members of the Pol X family in end-joining and suggests surprising complexity in the control of how these different polymerases are employed in this pathway...
  34. ncbi Increased junctional diversity in fetal B cells results in a loss of protective anti-phosphorylcholine antibodies in adult mice
    C L Benedict
    Department of Microbiology, University of Alabama at Birmingham, 35294 3300, USA
    Immunity 10:607-17. 1999
    ..These results show that maintenance of lower Ig diversity in early life is essential for the acquisition of a complete functional adult repertoire...
  35. ncbi Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination
    Jamie A E Repasky
    Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Immunol 172:5478-88. 2004
    ..We consider these results in light of the recently reported structure of the catalytic region of TdT...
  36. ncbi Isolation of putative promoter region for human terminal deoxynucleotidyltransferase gene
    O Koiwai
    Institute for Developmental Research, Aichi Prefectural Colony, Japan
    Biochem Biophys Res Commun 154:91-100. 1988
    ..The typical octamer of 5'-ATGCAAAT and three octamer-like sequences, which are involved in the transcription of lymphoid-specific genes, were detected at 212, 293, 347 and 770 bp upstream from the first cap site...
  37. pmc Terminal deoxynucleotidyl transferase: the story of a misguided DNA polymerase
    Edward A Motea
    Department of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Biochim Biophys Acta 1804:1151-66. 2010
    ..Finally, the biomedical role of this specialized DNA polymerase is discussed focusing on its involvement in cancer development and its use in biomedical applications such as labeling DNA for detecting apoptosis...
  38. ncbi Human terminal deoxynucleotidyl transferases as novel targets for anticancer chemotherapy
    Roberto Di Santo
    Istituto Pasteur Fondazione Cenci Bolognetti, Dip di Studi Farmaceutici, Facolta di Farmacia, Universita di Roma La Sapienza, Italy
    Curr Med Chem 13:2353-68. 2006
    ..In this review, we will summarize the recent advances in the synthesis and characterization of the first classes of specific inhibitors of mammalian terminal transferases and their potential applications...
  39. ncbi Mice lacking TdT: mature animals with an immature lymphocyte repertoire
    S Gilfillan
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Strasbourg, France
    Science 261:1175-8. 1993
    ..Thus, switch-on of the TdT gene during the first week after birth provokes an even greater expansion of lymphocyte receptor diversity than had previously been thought...
  40. pmc Molecular cloning of human terminal deoxynucleotidyltransferase
    R C Peterson
    Proc Natl Acad Sci U S A 81:4363-7. 1984
    ..These results provide unambiguous evidence that the human terminal transferase sequence has been cloned...
  41. pmc Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells
    O Koiwai
    Nucleic Acids Res 14:5777-92. 1986
    ..Bovine TdT expressed in COS7 cells by nearly full-length cDNA clone was localized in the nucleus and the translational product of pOK103 lacking the nuclear-localization-sequence was localized in the cytoplasm...
  42. ncbi Isolation and characterization of the Xenopus terminal deoxynucleotidyl transferase
    A Lee
    Department of Biology, New York University, New York 10003
    J Immunol 152:4500-7. 1994
    ....
  43. pmc T-cell specific avian TdT: characterization of the cDNA and recombinant enzyme
    B Yang
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina at Chapel Hill 27599 7260, USA
    Nucleic Acids Res 23:2041-8. 1995
    ..These data suggest that while TdT is probably involved in N region addition in chicken T-cell receptor genes, it is unlikely to play a role in diversification of immunoglobulin genes...
  44. pmc Differential splicing in mouse thymus generates two forms of terminal deoxynucleotidyl transferase
    N Doyen
    Institut Pasteur, Unité de Génétique et Biochimie du Développement, URA CNRS 361, Departement d Immunologie, Paris, France
    Nucleic Acids Res 21:1187-91. 1993
    ..Expression of this cDNA in mammalian cells shows that it encodes a functional protein capable of catalysing N region insertions at the recombination junction of an episomic recombination substrate...
  45. ncbi Terminal deoxynucleotidyl transferase. catalysis of DNA (oligodeoxynucleotide) phosphorylation
    A A Krayevsky
    Department of Chemical and Biological Analysis, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Str, Moscow, Russia
    Pharmacol Ther 85:165-73. 2000
    ..It has also been shown that TDT catalyzes primer extension with dinucleoside 5',5'-tetraphosphates as substrates...
  46. pmc The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene
    K Hahm
    Howard Hughes Medical Institute, University of California School of Medicine, Los Angeles 90024 1662
    Mol Cell Biol 14:7111-23. 1994
    ..These data reveal that the LyF-1 protein is encoded by specific mRNAs derived from the alternatively-spliced Ikaros gene, suggesting that this gene may be important for the early stages of both B- and T-lymphocyte development...
  47. ncbi Expression of CD5 on hematogones in a 7-year-old girl with Shwachman-Diamond syndrome
    T M Jelic
    Department of Pathology, Charleston Area Medical Center, WV 25304, USA
    Pediatr Dev Pathol 4:505-11. 2001
    ....
  48. ncbi Pre-steady-state kinetic studies of the fidelity of human DNA polymerase mu
    Michelle P Roettger
    Department of Biochemistry, Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 43:13827-38. 2004
    ..In addition to a slow and distributive DNA polymerase activity, Polmu was identified to possess a weak strand-displacement activity. The potential biological roles of Polmu are discussed...
  49. ncbi Acute myelogenous leukemia with t(8;21)--identification of a specific immunophenotype
    Haytham Khoury
    Leukemia BMT Program of British Columbia, Vancouver, Canada
    Leuk Lymphoma 44:1713-8. 2003
    ....
  50. pmc Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases
    G Martin
    Department of Cell Biology, University of Basel, Basel, Switzerland
    EMBO J 15:2593-603. 1996
    ..This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor...
  51. ncbi A novel DNA topoisomerase inhibitor: dehydroebriconic acid, one of the lanostane-type triterpene acids from Poria cocos
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science and High Technology Research Center, Kobe Gakuin University, Kobe, Hyogo 651 2180, Japan
    Cancer Sci 95:354-60. 2004
    ..6 and 38.4 microM, respectively. The cells were halted at the G1 phase in the cell cycle. The relation between the structure of triterpene acids and their inhibitory activities is discussed...
  52. ncbi Antibody repertoire development in fetal and neonatal piglets. VI. B cell lymphogenesis occurs at multiple sites with differences in the frequency of in-frame rearrangements
    Marek Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Doly 183, 549 22 Novy Hradek, Czech Republic
    J Immunol 170:1781-8. 2003
    ..We propose the latter to result from differential selection or a developmentally dependent change in the intrinsic mechanism of VDJ rearrangement...
  53. ncbi Maturation-associated immunophenotypic abnormalities in bone marrow B-lymphocytes in myelodysplastic syndromes
    Elisangela Ribeiro
    Hematology and Hemotherapy Center, State University of Campinas, Brazil
    Leuk Res 30:9-16. 2006
    ..Studies performed with purified well-characterized B-cells may further elucidate these abnormalities...
  54. ncbi 5-(Hydroxymethyl)-2-furfural: a selective inhibitor of DNA polymerase lambda and terminal deoxynucleotidyltransferase
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi ku, Kobe, Hyogo 651 2180, Japan
    Arch Biochem Biophys 446:69-76. 2006
    ..On the basis of these results, HMF was suggested to bind to the pol beta-like region of pol lambda and TdT...
  55. ncbi Four-color flow cytometric investigation of terminal deoxynucleotidyl transferase-positive lymphoid precursors in pediatric bone marrow: CD79a expression precedes CD19 in early B-cell ontogeny
    M N Dworzak
    Children s Cancer Research Institute, St Anna Kinderspital, Vienna, Austria
    Blood 92:3203-9. 1998
    ..This raises the chance to follow the sequential events heralding B-cell commitment in the most immature precursors by correlating phenotypic and genetic differentiation markers...
  56. ncbi Expression of CD24 on CD19- CD79a+ early B-cell progenitors in human bone marrow
    Elena Israel
    Flow Cytometry Unit, Soroka University Medical Center, Israel
    Cell Immunol 236:171-8. 2005
    ..e., displayed antigenic properties of early B-cell progenitors. Our data indicate that CD19- early B-cell progenitors in human BM express CD24, and that the expression of CD24 in human B-cell development precedes the expression of CD19...
  57. pmc Both LyF-1 and an Ets protein interact with a critical promoter element in the murine terminal transferase gene
    P Ernst
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, UCLA School of Medicine 90024 1662
    Mol Cell Biol 13:2982-92. 1993
    ..The role of LyF-1 is less clear; it might act in conjunction with the Ets protein bound at the D' element or it might be unnecessary for D' activity...
  58. pmc The c-myc protein represses the lambda 5 and TdT initiators
    S Mai
    Basel Institute for Immunology, Switzerland
    Nucleic Acids Res 23:1-9. 1995
    ..The significance of c-myc binding to the respective initiator was analysed by overexpressing c-myc in co-transfection assays. Under these conditions the transcriptional activity of both the lambda 5 and the TdT initiator was repressed...
  59. pmc Mutations occur in the Ig Smu region but rarely in Sgamma regions prior to class switch recombination
    Carol E Schrader
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655 0122, USA
    EMBO J 22:5893-903. 2003
    ..Finally, we find that mice expressing a transgene for terminal deoxynucleotidyl transferase (TdT) have nucleotide insertions at S-S junctions, indicating that the recombining DNA ends are accessible to end-processing enzyme activities...
  60. pmc B-cell precursors differentiated from cord blood CD34+ cells are more immature than those derived from granulocyte colony-stimulating factor-mobilized peripheral blood CD34+ cells
    Y Hirose
    Department of Infectious Diseases, Nagoya University School of Medicine, Nagoya, Japan
    Immunology 104:410-7. 2001
    ..These results indicated that immature features of pre-B cells from CB, such as lack of TdT expression, and a short N region and few productive rearrangements in the IgH gene, might cause the delay in mature B-cell production...
  61. pmc Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells
    Cuixia Tian
    Department of Pediatrics, Vanderbilt University Medical Center, 21st Avenue South, Nashville, TN 37232, USA
    Mol Immunol 44:2173-83. 2007
    ..These data suggest distinct functional mechanisms underlying selection of this unique subset of un-class-switched memory B cells...
  62. ncbi Unique pattern of nuclear TdT immunofluorescence distinguishes normal precursor B cells (Hematogones) from lymphoblasts of precursor B-lymphoblastic leukemia
    Matthew T Hurford
    Department of Pathology and Laboratory Medicine, Temple University Medical Center, Philadelphia, PA 19140, USA
    Am J Clin Pathol 129:700-5. 2008
    ..The nuclear pattern of immunofluorescence using antibodies to TdT is an effective method for distinguishing hematogones from leukemic blasts...
  63. ncbi Origins of peripheral B cells in IL-7 receptor-deficient mice
    David G T Hesslein
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06519, USA
    Mol Immunol 43:326-34. 2006
    ..We conclude that the majority of splenic B cells in IL-7Ralpha-deficient mice originate from the bone marrow and not the fetal liver...
  64. ncbi Normal D-JH rearranged products of the IgH gene in SCID mouse bone marrow
    R Araki
    Cell Biology Laboratory, Department of Radiobiology, Radiation Effects Research Foundation, 8 6 Nakagawa 1 chome, Nagasaki 850, Japan
    Int Immunol 8:1045-53. 1996
    ..Furthermore, the features of rearranged products isolated from SCID bone marrow cells were remarkably similar to those from leaky SCID mice...
  65. ncbi Prognostic relevance of immunophenotyping in 379 patients with acute myeloid leukemia
    Hong Chang
    Department of Laboratory Hematology, Princess Margaret Hospital University Health Network, University of Toronto, Toronto, Ont, Canada M5G 2M9
    Leuk Res 28:43-8. 2004
    ..Defined by number of factors, this score system may help to stratify AML patients to alternative treatment for better outcome...
  66. ncbi Rare oral cavity presentation of a B-cell lymphoblastic lymphoma. A case report and review of the literature
    Darren P Cox
    Department of Orofacial Sciences, University of California, San Francisco, California, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103:814-9. 2007
    ..This case emphasizes the importance of recognizing rare entities that may present in the oral cavity and the impact of the disease and its management...
  67. ncbi CD99 expression in T-lineage ALL: implications for flow cytometric detection of minimal residual disease
    M N Dworzak
    Children s Cancer Research Institute, St Anna Children s Hospital, Vienna, Austria
    Leukemia 18:703-8. 2004
    ..002% of nucleated BM cells in patients with non-T ALL). We conclude that CD99 is a highly informative tool for MRD detection in T-ALL, bearing the advantage of surface expression in contrast to TdT...
  68. ncbi Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species
    J Sinkora
    Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
    Vet Immunol Immunopathol 119:156-62. 2007
    ..Altogether, our study show that carefully selected polyclonal antibodies available on the market may possess broad cross-reactivity with important applications in veterinary research...
  69. ncbi Immunoreactivity of MIC2 (CD99) and terminal deoxynucleotidyl transferase in bone marrow clot and core specimens of acute myeloid leukemias and myelodysplastic syndromes
    Loveleen C Kang
    Division of Hematopathology, Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
    Arch Pathol Lab Med 130:153-7. 2006
    ..Although this study speculated that MIC2 expression was probably not limited to TdT-positive AML, there was no comparison with TdT reactivity in this study...
  70. ncbi Mature B-cell acute lymphoblastic leukemia with t(9;11) translocation: a distinct subset of B-cell acute lymphoblastic leukemia
    Lawrence Tsao
    Department of Pathology, Columbia University, New York, NY 10032, USA
    Mod Pathol 17:832-9. 2004
    ..Identification of additional cases will further clarify the significance of MLL rearrangements in mature B-ALL...
  71. ncbi TUNEL assay as a measure of chemotherapy-induced apoptosis
    Robert Wieder
    University of Medicine and Dentistry of New Jersey, Division Medical Oncology Hematology, Newark, USA
    Methods Mol Med 111:43-54. 2005
    ..It lists the reagents, procedures for cell and tissue handling, labeling techniques, caveats, and appropriate controls for labeling 3'OH termini with TdT and quantifying the results...
  72. ncbi Inhibitory effect of dipeptide alcohol derivatives containing mercapto group on eukaryotic DNA polymerase alpha
    Ikuo Kato
    Yanaihara Institute Inc, Awakura, Fujinomiya, Shizuoka 418 0011, Japan
    Int J Mol Med 16:653-9. 2005
    ..The relationships between the structures of dipeptide alcohol and the inhibition of eukaryotic DNA polymerases are discussed...
  73. ncbi Distinct and opposite activities of human terminal deoxynucleotidyltransferase splice variants
    To Ha Thai
    Division of Developmental and Clinical Immunology, University of Alabama, Birmingham 35294, USA
    J Immunol 173:4009-19. 2004
    ..Finally, the newly discovered hTdT isoforms should be considered in future screening of human leukemias...
  74. ncbi Evaluation of CD7 and terminal deoxynucleotidyl transferase (TdT) expression in CD34+ myeloblasts from patients with myelodysplastic syndrome
    P Font
    Department of Haematology, Clinica Moncloa, Madrid, Spain
    Leuk Res 30:957-63. 2006
    ..Therefore, CD7 and TdT may help to establish the diagnosis of MDS and, TdT expression also seems to be a useful marker in distinguishing risk groups...
  75. ncbi Nonpositive terminal deoxynucleotidyl transferase in pediatric precursor B-lymphoblastic leukemia
    Lanting Liu
    Department of Pathology, University of Colorado School of Medicine, Denver, USA
    Am J Clin Pathol 121:810-5. 2004
    ..0019; respectively). These results indicate that nonpositive TdT does not rule out a diagnosis of ALL and suggest that TdT(np) B-cell ALL might be associated with CD10- and CD34- disease, a high WBC count, and MLL gene rearrangement...
  76. ncbi Flow cytometric analysis of acute leukemias. Diagnostic utility and critical analysis of data
    Zahid Kaleem
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA
    Arch Pathol Lab Med 127:42-8. 2003
    ..The essential role and caveats of flow cytometry in that regard, however, have received little scrutiny...
  77. ncbi Quinolone antibiotics inhibit eucaryotic DNA polymerase alpha and beta, terminal deoxynucleotidyl transferase but not DNA ligase
    R Rusquet
    Biochem Biophys Res Commun 121:762-9. 1984
    ..The significance of these results is discussed in terms of the possible involvement of the enzymes in the respective DNA replication and repair processes...
  78. ncbi Terminal deoxynucleotidyl transferases from elasmobranchs reveal structural conservation within vertebrates
    Simona Bartl
    Moss Landing Marine Laboratories, 8272 Moss Landing Road, CA 95039, Moss Landing, USA
    Immunogenetics 55:594-604. 2003
    ..Thus, unlike other molecules of adaptive immunity, TdT is a member of an ancient gene family with a clear gene phylogeny and a high degree of similarity, which implies the existence of TdT ancestors in jawless fishes and invertebrates...
  79. ncbi Structure-activity relationships of untenone A and its derivatives for inhibition of DNA polymerases
    Fumiyo Saito
    Frontier Research Center for Genome and Drug Discovery, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem Lett 14:1975-7. 2004
    ..The syntheses of both compounds and the structure-activity relationships of untenone A derivatives are described...
  80. ncbi Characterization of terminal deoxynucleotidyl transferase and polymerase mu in zebrafish
    Susann Beetz
    Biology Department, Massachusetts Institute of Technology, Cambridge, MA, USA
    Immunogenetics 59:735-44. 2007
    ..The characterization of zebrafish TdT and polmu provide new insights in fish lymphopoiesis and addresses the importance and evolution of TdT and polmu themselves...
  81. ncbi [Expression and function of terminal deoxynucleotidyl-transferase and discovery of novel DNA polymerase mu]
    Noriko Shimazaki
    Faculty of Science and Technology, Department of Applied Biological Science, Science University of Tokyo, Noda, Chiba 278 8510
    Seikagaku 74:227-32. 2002
  82. ncbi Synthetic analogues of the manzamenones and plakoridines which inhibit DNA polymerase
    Jeremy R Doncaster
    The School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Bioorg Med Chem Lett 16:2877-81. 2006
    ..Many of the target compounds show potent inhibition of DNA polymerases alpha and beta and human terminal deoxynucleotidyl transferase (TdT)...
  83. ncbi Identification and characterization of human DNA polymerase beta 2, a DNA polymerase beta -related enzyme
    K Nagasawa
    Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474 8522, Japan
    J Biol Chem 275:31233-8. 2000
    ..These results indicate that Pol beta2 is a Pol beta-related DNA polymerase with a BRCT motif that is dispensable for its polymerase activity...
  84. ncbi Selective inhibitors of terminal deoxyribonucleotidyltransferase (TdT): baicalin and genistin
    Yukinobu Uchiyama
    Department of Applied Biological Science and Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Biochim Biophys Acta 1725:298-304. 2005
    ..The compounds may, therefore, bind directly to the primer-binding site of TdT and simultaneously disturb dNTP substrate incorporation into the primer. Genistin and baicalin should prove to be useful agents for studying TdT...
  85. ncbi DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region
    Noriko Shimazaki
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Genes Cells 10:705-15. 2005
    ..Through direct binding, PCNA suppressed the distributive nucleotidyltransferase activity of Pol lambda. Pol micro, which also belongs to the family X of DNA polymerases, binds to PCNA by a pivotal amino acid residue...
  86. ncbi Expression of the recombination-activating genes in extrafollicular lymphocytes but no apparent reinduction in germinal center reactions in human tonsils
    Nadine Meru
    Pathologisches Institut, Friedrich Alexander Universitat, Erlangen, Germany
    Blood 99:531-7. 2002
    ..This may reflect an increased influx of RAG(+)/TdT(+) cells as a result of higher antigenic stimulation at this site. Alternatively, this observation may indicate that the tonsils are an additional site of lymphocyte ontogeny...
  87. ncbi Ikaros is critical for B cell differentiation and function
    Peggy Kirstetter
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, France
    Eur J Immunol 32:720-30. 2002
    ..In the periphery, mature B cells exhibit a lower activation threshold but form fewer germinal centers in response to antigenic stimulation. Our results show that Ikaros controls multiple aspects of B cell differentiation and function...
  88. ncbi Maturation of B cell precursors is impaired in thymic-deprived nude and old mice
    P Szabo
    Division of Geriatrics and Gerontology, Cornell University Medical College, New York 10021, USA
    J Immunol 161:2248-53. 1998
    ..We conclude that the impaired development of B cells within the bone marrow of thymic-deprived nude and old mice can be reversed, at least in part, by the administration of rIL-16...
  89. ncbi Modulation of experimental colon tumorigenesis by types and amounts of dietary fatty acids
    C V Rao
    Division of Nutritional Carcinogenesis, American Health Foundation, Valhalla, New York 10595, USA
    Cancer Res 61:1927-33. 2001
    ....
  90. ncbi Evidence that carbonyl stress by methylglyoxal exposure induces DNA damage and spindle aberrations, affects mitochondrial integrity in mammalian oocytes and contributes to oocyte ageing
    Carla Tatone
    Department of Health Sciences, University of L Aquila, Via Vetoio, 67100 L Aquila, Italy
    Hum Reprod 26:1843-59. 2011
    ..Whether and how MG and AGE affect young and aged oocytes at the cellular level is unknown...
  91. ncbi Action and mechanism of Fas and Fas ligand in immune escape of gallbladder carcinoma
    Li Ning Xu
    Department of General Surgery of Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
    World J Gastroenterol 11:3719-23. 2005
    ..To study the role of Fas and Fas ligand (FasL) in biological behaviors of gallbladder carcinoma, and their correlated action and mechanism in tumor escape...
  92. ncbi Tumor necrosis factor-alpha induces apoptosis of enterocytes in mice with fulminant hepatic failure
    Hong Li Song
    Department of Infectious Diseases, The Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
    World J Gastroenterol 11:3701-9. 2005
    ..To explore the alterations of intestinal mucosa morphology, and the effects of tumor necrosis factor alpha (TNFalpha) on enterocyte apoptosis in mice with fulminant hepatic failure (FHF)...
  93. ncbi Cell death induced by down-regulation of heat shock protein 70 in lung cancer cell lines is p53-independent and does not require DNA cleavage
    Steffen Frese
    Division of General Thoracic Surgery, Department of Clinical Research, University Hospital, Murtenstrasse 35, Room C807, CH 3010 Berne, Switzerland
    J Thorac Cardiovasc Surg 126:748-54. 2003
    ..We therefore evaluated whether selective depletion of heat shock protein 70 induces cell death in lung cancer cells...
  94. ncbi [In vitro observation on the apoptosis induced by H2O2 in protoscolex of Echinococcus granulosus]
    Jin Feng Kang
    Xinjiang Medical University, Urumqi 830054, China
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 26:332-7. 2008
    ..To explore the apoptosis induced by hydrogen peroxide (H2O2) in protoscolex of Echinococcus granulosus...
  95. ncbi Pilot study of dietary fat restriction and flaxseed supplementation in men with prostate cancer before surgery: exploring the effects on hormonal levels, prostate-specific antigen, and histopathologic features
    W Demark-Wahnefried
    Division of Urologic Surgery, Duke University Medical Center, Durham, North Carolina, USA
    Urology 58:47-52. 2001
    ..Further study is needed to determine the benefit of this dietary regimen as either a complementary or preventive therapy...
  96. ncbi Histopathology, immunohistochemistry, in situ apoptosis, and ultrastructure characterization of the digestive and lymphoid organs of new type gosling viral enteritis virus experimentally infected gosling
    S Chen
    Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya an, Sichuan Province, 625014, China
    Poult Sci 89:668-80. 2010
    ..This work may shed light on the pathogenesis of new type gosling viral enteritis and put new insight into the pathogenesis of goose adenovirus...
  97. ncbi Keratinocytes in the depigmented epidermis of vitiligo are more vulnerable to trauma (suction) than keratinocytes in the normally pigmented epidermis, resulting in their apoptosis
    A Y Lee
    Department of Dermatology, Eulji University School of Medicine, 280 1 Hagye 1 dong, Nowon Gu, Seoul 139 711, Korea
    Br J Dermatol 151:995-1003. 2004
    ..However, in autologous epidermal grafting, depigmentation of the donor (normally pigmented) site from a suction blister is rare, even in cases displaying failure of repigmentation at the recipient (depigmented) site...
  98. ncbi Antibodies, immunoglobulin genes and the bursa of Fabricius in chicken B cell development
    Michael J H Ratcliffe
    Department of Immunology, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada
    Dev Comp Immunol 30:101-18. 2006
    ..This review focuses on the differences between B cell development in the chicken as compared to rodent and primate models...
  99. ncbi Infiltrating polymorphonuclear leukocytes and apoptotic bodies derived from hepatocytes but not from ballooning hepatocytes containing Mallory bodies show nuclear DNA fragmentation in alcoholic hepatitis
    T Takahashi
    Third Department of Internal Medicine, Niigata University School of Medicine, Niigata, Japan
    Alcohol Clin Exp Res 24:68S-73S. 2000
    ..The mechanism of liver cell death in alcoholic hepatitis is still controversial. There is evidence that polymorphonuclear leukocytes (PMNs) which infiltrate liver parenchyma are involved in liver cell death...
  100. ncbi Thiopental-induced apoptosis in lymphocytes is independent of CD95 activation
    Marius Keel
    Department of Trauma Surgery, Division of Trauma Surgery, University Hospital Zurich, Switzerland
    Anesthesiology 103:576-84. 2005
    ..However, barbiturate-mediated neuroprotection correlates with lymphopenia, which increases the risk of infection. The mechanisms by which barbiturates lead to lymphopenia remain to be determined...
  101. ncbi Experimental study of controlled fluid resuscitation in the treatment of severe and uncontrolled hemorrhagic shock
    Yuan qiang Lu
    Department of Emergency and General Surgery, Sir Run Run Shaw Hospital Affiliated to Medical College, Zhejiang University, Hangzhou, China
    J Trauma 63:798-804. 2007
    ..This study was conducted to evaluate the general and pathophysiologic effects of controlled fluid resuscitation in the treatment of severe and uncontrolled hemorrhagic shock...

Research Grants62

  1. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..Present study attempts to identify cyanidin-3-glucoside, a plant-derived compound, as a mechanism-based preventive agent against UV-induced skin cancers. ..
  2. Somatic Hypermutation in SLE
    Lawrence J Wysocki; Fiscal Year: 2013
    ..Results of this project will provide basic information about the natural history of autoreactive B cells, which is essential if we are to understand, control and prevent systemic autoimmune disease. ..
  3. Bcl-2, A Key Factor for Dentinogenesis and Tooth Damage Repair
    Wenjian Zhang; Fiscal Year: 2010
    ..It is expected to generate new interventions to preserving the vitality and functions of teeth injured by infections or trauma, and contributing to fabricated teeth via bioengineering. ..
  4. Aptosis in an ovine model of intrauterine growth restriction (IUGR)
    Juan A Arroyo; Fiscal Year: 2010
    ....
  5. Apoptosis in an Ovine Model of Intrauterine Growth Restriction (IUGR)
    Juan A Arroyo; Fiscal Year: 2013
    ..To study apoptosis in-vitro we will determine the effects of hyperthermia and hypoxia on cultured placental BNCs. ..
  6. Osteocyte reaction to decreased bone fluid flow
    Shijing Qiu; Fiscal Year: 2003
    ..The spatial relationship between osteoclastic resorption and osteocyte degeneration will be determined in the sections with toluidine blue and TUNEL stain. ..
  7. Association between the Y chromosome and androgens
    Kathryn Sandberg; Fiscal Year: 2007
    ..abstract_text> ..
  8. Hormonal Regulation of the Cervix During Pregnancy
    ORRIN SHERWOOD; Fiscal Year: 2006
    ..Treatment with RU 486 alone and in combination with exogenous relaxin will be done at term with relaxin-deficient rats. ..
  9. RETINOIDS & RECEPTORS IN BLADDER CANCER CHEMOPREVENTION
    Changping Zou; Fiscal Year: 2003
    ..The results of these studies are expected to increase our understanding of the mechanisms of action and potential usefulness of new retinoids in bladder cancer chemoprevention. ..
  10. MOLECULAR PATHOLOGY OF LEUKEMIA/LYMPHOID MARKER
    KAREN CHANCELLOR; Fiscal Year: 1993
    ..Integration of these data will yield a comprehensive picture of structure/ function correlates of this gene in normal and leukemic cells...
  11. Antidepressants and Fibroblast Growth Factor
    Italo Mocchetti; Fiscal Year: 2006
    ..Data from this proposal may help elucidate the therapeutic efficacy of antidepressants in affective disorders. [unreadable] [unreadable]..
  12. CALCIUM MEDIATED APOPTOSIS AND CALBINDIN-D IN BETA CELLS
    WILLIAM RHOTEN; Fiscal Year: 1999
    ..The long term goal is to integrate calbindin D28k gene expression into a therapeutic strategy that will protect the B-cell from immune-system-mediated destruction and prevent IDDM. ..
  13. MULTIPURPOSE ARTHRITIS CENTER
    Charles Christian; Fiscal Year: 1980
    ....
  14. Isoforms of Human Terminal Transferase and Lymphopoiesis
    John Kearney; Fiscal Year: 2007
    ..abstract_text> ..
  15. SENESCENE AND PREB CELL DEVELOPMENT
    Richard Riley; Fiscal Year: 2003
    ..The experiments in this application will provide a cellular and molecular basis for understanding the temporal loss of B lymphopoiesis and altered generation of VH repertoire during senescence. ..
  16. FAS AND FASL IN AUTOIMMUNE ENCEPHALOMYELITIS
    Youhai Chen; Fiscal Year: 2000
    ..This will be addressed by investigating the effects of FasL deficiency in neural compartment using our adoptive transfer model. Both normal and RAG-1 deficient recipient mice will be used. ..
  17. Noninvasive Monitoring of In Vivo Drug Release
    Yoshinori Kato; Fiscal Year: 2009
    ..We anticipate that our innovative technique using MRI/MRSI will provide a noninvasive monitoring of drug release and subsequent distribution in the tumor. ..
  18. The 5-HT1A Receptor and Brain Development
    Probal Banerjee; Fiscal Year: 2005
    ..This project will delineate a novel pathway that could play a key role in early brain development. Knowledge of this pathway will help in designing better therapies to combat developmental brain disorders. ..
  19. Role of hPso4 in Double Strand Break Repair
    Beverly Mitchell; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  20. Vanadocenes as a New Class of Spermicidal Drugs
    OSMOND D CRUZ; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. PHI-443: Novel Non-Spermicidal Anti-HIV Microbicide
    OSMOND D CRUZ; Fiscal Year: 2003
    ..Specific Aims 1 and 2 may provide the foundation for the clinical development of PHI-443 in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
  22. Stampidine: A Novel Broad-Spectrum Anti-HIV Microbicide
    OSMOND D CRUZ; Fiscal Year: 2005
    ..Specific Aims 1 and 2 may provide the foundation for the clinical development of STAMP in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
  23. Phase II SBIR: WHI-07: A Novel Dual-Function Microbicide
    OSMOND D CRUZ; Fiscal Year: 2005
    ....
  24. PHI 346: A Novel Dual-Function Microbicide
    OSMOND D CRUZ; Fiscal Year: 2003
    ..Following successful completion of the Phase I in vivo efficacy studies, gel formulation of PHI-346 will be further explored as a vaginal dual-function microbicide in Phase II. ..
  25. NOVEL CONTRACEPTIVES WITH ANTI-HIV ACTIVITY
    OSMOND D CRUZ; Fiscal Year: 2001
    ..The preclinical data on in vivo efficacies of WHI-05 and WHI-07 will be essential to further explore the utility of these novel drugs for clinical studies in human patients. ..
  26. METVAN: A Novel Anticancer Agent
    OSMOND D CRUZ; Fiscal Year: 2003
    ..The knowledge gained from these studies described under Specific Aims 1-2 is expected to facilitate the design of innovative treatment regimens employing METVAN for metastatic breast cancer and brain tumor patients. ..
  27. FLP-102: Rationally Engineered Antiviral Protein
    OSMOND D CRUZ; Fiscal Year: 2003
    ..The preclinical data on FLP-102 will be essential to further explore the utility of this novel recombinant PAP mutant for Phase II. ..
  28. Mechanisms of Cardiac Regeneration
    DINENDER SINGLA; Fiscal Year: 2008
    ..The results from the proposed projects will have long-term implications for future cell-based therapy to treat heart disease (s) using embryonic stem cells or potentially other donor cells. [unreadable] [unreadable] [unreadable]..
  29. Divergent Pathways of Cell Death after Brain Injury
    Robert S B Clark; Fiscal Year: 2010
    ..Several unique pathways contribute to overall brain damage. This application seeks to define clinically important pathways, and design novel, sex-based strategies that improve neurological outcome after brain injury. ..
  30. Mechanisms of SCI Recovery after hCNS Stem Cell Grafts
    Aileen Anderson; Fiscal Year: 2008
    ....
  31. AIRWAY PARTICLE CLEARANCE--ROLE OF BRONCHIAL CIRCULATION
    Elizabeth Wagner; Fiscal Year: 2001
    ..Furthermore, the results will contribute to an understanding of the factors that both limit and enhance uptake and distribution of aerosols. ..
  32. DIABETES IN HEMOCHROMATOSIS: PREVALENCE AND MECHANISMS
    Donald McClain; Fiscal Year: 2007
    ..These results will guide future studies to determine the role of these phenomena in type 2 diabetes. ..
  33. PROTEOMIC ANALYSIS OF HIPPOCAMPAL HYPOXIC VULNERABILITY
    Jon Klein; Fiscal Year: 2003
    ..These studies will characterize changes in protein expression and post-translational modifications that may have important implications for cell survival and/or adaption to intermittent and sustained hypoxia. ..
  34. STRUCTURE/FUNCTION OF NERVE GROWTH FACTOR/NEUROTROPHINS
    KENNETH NEET; Fiscal Year: 2009
    ..abstract_text> ..
  35. Effects of intracellular angiotensin II
    Julia Cook; Fiscal Year: 2009
    ..abstract_text> ..
  36. Gender-Specific Treatment of Pediatric Cardiac Arrest
    Robert Clark; Fiscal Year: 2009
    ..abstract_text> ..
  37. DNA Damage/Repair and Cell Death
    Lee Martin; Fiscal Year: 2009
    ....
  38. Angiogenesis in the Lung
    Elizabeth Wagner; Fiscal Year: 2010
    ..In an animal model of chronic pulmonary thromboembolic disease, this project will determine the inflammatory cytokines and cells responsible for new vessel growth, and pharmacological and interventional inhibitors. ..
  39. Meningioma: Risk Factors and Quality of Life
    JOSEPH LEO WIEMELS; Fiscal Year: 2010
    ..This study represents the first concentrated effort to examine environmental and genetic risk factors for meningioma. ..
  40. SHORT-TERM RESEARCH TRAINING FOR MINORITY STUDENTS
    JOHN RICHBURG; Fiscal Year: 2006
    ....
  41. Apoptosis inducing factor in cardiac apoptosis
    Peter M Kang; Fiscal Year: 2010
    ..We will then explore a mechanism and a strategy for inhibiting AIF activation using PARP-1 inhibition in I/R-induced cardiac apoptosis in vivo. ..
  42. Bacterial Colonization and Development of Innate Intestinal Host Defense
    Patricia Lin; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  43. Cellular Mechanisms of Preterm White Matter Injury
    STEPHEN BACK; Fiscal Year: 2003
    ....
  44. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  45. BRAIN GALANIN RECEPTORS AND EPILEPSY
    Andrey Mazarati; Fiscal Year: 2006
    ..abstract_text> ..
  46. Glycosaminoglycans For IC Pathophysiology and Prognosis
    Vinata Lokeshwar; Fiscal Year: 2005
    ..Furthermore, it might yield a test or a combination of tests that can be used in the follow-up of IC patients and for monitoring 9 treatment responses. ..
  47. PROSTAGLANDINS AND GASTROINTESTINAL INTEGRITY
    Thomas Miller; Fiscal Year: 2006
    ....
  48. Control of Accessibility for V(D) J Recombination
    ANN FEENEY; Fiscal Year: 2006
    ..We will determine if other transcription factors such as Pax5 or Btk will induce rearrangement of other Ig loci, such as Vh or lambda. ..
  49. Pathobiology of HMG-CoA reductase inhibitors in diabetes
    Yashpal Kanwar; Fiscal Year: 2006
    ....
  50. Control Of Tcr Data And Tcr Alpha Rearrangement
    MICHAEL KRANGEL; Fiscal Year: 2005
    ..abstract_text> ..
  51. THE ROLE OF COMPLEMENT IN AUTOIMMUNITY
    Garnett Kelsoe; Fiscal Year: 2005
    ..Thus, we propose that the innate immune system controls both the frequency of peripheral, self-reactive B cells and their exposure to antigen. ..
  52. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2006
    ..abstract_text> ..
  53. CYCLOOXYGENASE AND PGE2 RECEPTOR FUNCTION IN SKIN CANCER
    Alice Pentland; Fiscal Year: 2004
    ..EP receptor effects on keratinocyte proliferation, apoptosis and differentiation will be studied as markers of cutaneous neoplasia. ..
  54. ROLE OF COMPLEMENT FACTOR B IN THE PATHOGENESIS OF SLE
    Gary Gilkeson; Fiscal Year: 2004
    ..These studies will provide new insight into the role of the alternative pathway in disease and potentially provide a new therapeutic target (Factor B) in immune complex mediated diseases. ..
  55. Vaccine responsiveness to Borrelia burgdorferi OspA
    Richard Flavell; Fiscal Year: 2007
    ..abstract_text> ..
  56. Mechanisms of Complement-Mediated Damage in SCI
    Aileen Anderson; Fiscal Year: 2007
    ....
  57. Xenogen IVIS-200 Bioluminescent Imaging System
    Alan Pollack; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  59. Molecular Determinants of Virulence Porcine Circovirus 2
    Steven Krakowka; Fiscal Year: 2007
    ..Collectively, these data will provide critical insight into pathogenic mechanisms of disease mediated by mammalian circoviruses and will provide key guidance for the future study of human circovirus pathogens. ..