fura 2

Summary

Summary: A fluorescent calcium chelating agent which is used to study intracellular calcium in many tissues. The fluorescent and chelating properties of Fura-2 aid in the quantitation of endothelial cell injury, in monitoring ATP-dependent calcium uptake by membrane vesicles, and in the determination of the relationship between cytoplasmic free calcium and oxidase activation in rat neutrophils.

Top Publications

  1. pmc Glutamate mobilizes [Zn2+] through Ca2+ -dependent reactive oxygen species accumulation
    Kirk E Dineley
    Department of Biology, Francis Marion University, Florence, SC, USA
    J Neurochem 106:2184-93. 2008
  2. ncbi Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
    Elisabeth M Froschauer
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University of Vienna, Campus Vienna Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
    FEMS Microbiol Lett 237:49-55. 2004
  3. ncbi Inhibition of multidrug/xenobiotic resistance transporter by MK571 improves dye (Fura 2) accumulation in crustacean tissues from lobster, shrimp, and isopod
    Ann Katrin Lüders
    Biologische Anstalt Helgoland, Alfred Wegener Institute for Polar and Marine Research in the Helmholtz Association, Helgoland, Germany
    Comp Biochem Physiol C Toxicol Pharmacol 150:368-71. 2009
  4. ncbi T-jump study of calcium binding kinetics of calcium chelators
    M Naraghi
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Cell Calcium 22:255-68. 1997
  5. pmc Effect of fura-2 on action potential-stimulated calcium release in cut twitch fibers from frog muscle
    P C Pape
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510 8026
    J Gen Physiol 102:295-332. 1993
  6. ncbi An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell
    J M McHugh
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Am J Physiol Cell Physiol 286:C342-8. 2004
  7. pmc A mathematical model predicts that calreticulin interacts with the endoplasmic reticulum Ca(2+)-ATPase
    Helen L Baker
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, United Kingdom
    Biophys J 82:582-90. 2002
  8. ncbi Blockade of mitochondrial Ca2+ uptake by mitochondrial inhibitors amplifies the glutamate-induced calcium response in cultured cerebellar granule cells
    B Khodorov
    Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow
    FEBS Lett 458:162-6. 1999
  9. ncbi Prevention of age-related dysregulation of calcium dynamics by estrogen in neurons
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School Medicine, P O Box 19626, Springfield, IL 62794 9626, USA
    Neurobiol Aging 27:306-17. 2006
  10. ncbi Time course of calcium transients derived from Fura-2 fluorescence measurements in single fast twitch fibres of adult mice and rat myotubes developing in primary culture
    A J Bakker
    School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia
    Cell Calcium 21:359-64. 1997

Detail Information

Publications256 found, 100 shown here

  1. pmc Glutamate mobilizes [Zn2+] through Ca2+ -dependent reactive oxygen species accumulation
    Kirk E Dineley
    Department of Biology, Francis Marion University, Florence, SC, USA
    J Neurochem 106:2184-93. 2008
    ..We conclude that glutamate-induced [Zn(2+)](i) transients are caused in part by [Ca(2+)](i)-induced reactive oxygen species that arises from both cytosolic and mitochondrial sources...
  2. ncbi Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
    Elisabeth M Froschauer
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University of Vienna, Campus Vienna Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
    FEMS Microbiol Lett 237:49-55. 2004
    The Mg2+ fluorescent dye mag-fura 2, entrapped in cells or organelles, has frequently been used for dual excitation ratio-metric determinations of free ionic Mg2+ concentrations in eukaryotic, mostly mammalian cells...
  3. ncbi Inhibition of multidrug/xenobiotic resistance transporter by MK571 improves dye (Fura 2) accumulation in crustacean tissues from lobster, shrimp, and isopod
    Ann Katrin Lüders
    Biologische Anstalt Helgoland, Alfred Wegener Institute for Polar and Marine Research in the Helmholtz Association, Helgoland, Germany
    Comp Biochem Physiol C Toxicol Pharmacol 150:368-71. 2009
    ..proteins (MRP) are one type of ATP-Binding-Cassette (ABC) transporters, which also transport dyes such as Fura 2, a calcium chelating fluorescence indicator...
  4. ncbi T-jump study of calcium binding kinetics of calcium chelators
    M Naraghi
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Cell Calcium 22:255-68. 1997
    ..EGTA and H-EDTA have small binding rate constants for calcium in the range of 3 x 10(6) M-1s-1 since, at pH 7.20, protons need to be dissociated from the chelators before they can bind calcium ions...
  5. pmc Effect of fura-2 on action potential-stimulated calcium release in cut twitch fibers from frog muscle
    P C Pape
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510 8026
    J Gen Physiol 102:295-332. 1993
    ..L. Csernoch, M. G. Klein, and M. F. Schneider. 1991. Biophysical Journal. 60:867-873) or a possible pharmacological effect of fura-2...
  6. ncbi An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell
    J M McHugh
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Am J Physiol Cell Physiol 286:C342-8. 2004
    ..In contrast, [Ca2+]avg,M2 follows [Ca2+]avg closely...
  7. pmc A mathematical model predicts that calreticulin interacts with the endoplasmic reticulum Ca(2+)-ATPase
    Helen L Baker
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, United Kingdom
    Biophys J 82:582-90. 2002
    ....
  8. ncbi Blockade of mitochondrial Ca2+ uptake by mitochondrial inhibitors amplifies the glutamate-induced calcium response in cultured cerebellar granule cells
    B Khodorov
    Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow
    FEBS Lett 458:162-6. 1999
    ..The reasons for a quantitative difference between [Ca2+]i responses observed in fura-2- and fura-2FF-loaded neurones are discussed...
  9. ncbi Prevention of age-related dysregulation of calcium dynamics by estrogen in neurons
    Gregory J Brewer
    Department of Neurology, Southern Illinois University School Medicine, P O Box 19626, Springfield, IL 62794 9626, USA
    Neurobiol Aging 27:306-17. 2006
    ..These results demonstrate age-associated dysregulation of [Ca2+]i homeostasis which was largely prevented by 17beta-estradiol with implications for estrogen/hormone therapy...
  10. ncbi Time course of calcium transients derived from Fura-2 fluorescence measurements in single fast twitch fibres of adult mice and rat myotubes developing in primary culture
    A J Bakker
    School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia
    Cell Calcium 21:359-64. 1997
    ..The correction process was found to have little effect on Ca2+ transients with rise time values greater than 10 ms, which included most of the Ca2+ transients measured in the myotubes...
  11. ncbi Safrole-induced cellular Ca2+ increases and death in human osteosarcoma cells
    Hsueh Chi Lin
    Department of Physical Medicine and Rehabilitation, St Joseph Hospital, Kaohsiung 802, Taiwan
    Pharmacol Res 54:103-10. 2006
    ..The safrole response involved Ca2+ influx and is modulated by protein kinase C. Furthermore, safrole can cause apoptosis in a Ca2+-independent manner...
  12. ncbi No evidence for bradykinin B1 receptors in rat dorsal root ganglion neurons
    M Brand
    Institute of Physiology, , , , Germany
    Neuroreport 12:3165-8. 2001
    ..Normal external solution applied at this flow rate constituted a mechanical stimulus causing a response in some neurons. Thus, in comparable set-ups mechanosensitivity has first to be tested to avoid masking effects...
  13. ncbi Characterization of histamine-induced increases in intracellular free Ca2+ concentrations in Chang liver cells
    J S Cheng
    Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
    J Recept Signal Transduct Res 21:1-9. 2001
    ..In Ca2+-free medium, after treatment with 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor), 5 microM histamine failed to increase [Ca2+]i. Histamine (5 microM)-induced intracellular Ca2+ release was abolished..
  14. ncbi Changes in functioning of rat submandibular salivary gland under streptozotocin-induced diabetes are associated with alterations of Ca2+ signaling and Ca2+ transporting pumps
    N V Fedirko
    Biological Department, I Franko Lviv National University, 4 Grushevsky St, Lviv 79005, Ukraine
    Biochim Biophys Acta 1762:294-303. 2006
    ....
  15. ncbi Endothelin-1 activates ET(A) receptors to increase intracellular calcium in model sensory neurons
    Q L Zhou
    Molecular Neurobiology of Pain, and 1Sensory Neurophysiology Laboratories of the Pain Research Center, Department of Anesthesiology, Perioperative and Pain Management, Brigham and Women's Hospital, Boston, MA 02115, USA
    Neuroreport 12:3853-7. 2001
    ..These results demonstrate that ET-1 induces dose- and ETA receptor-dependent release of Ca2+in in nociceptor-like neurons, and permit further examination of the pathways that underlie ET-1-induced pain signaling...
  16. ncbi Stimulated increase in free cytosolic Ca(2+) and protein kinase C activity in human cerebrocortical synaptosomes
    Morten C Moe
    Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet University Hospital, N 0027 Oslo, Norway
    Brain Res 924:116-9. 2002
    ..This method is thus well suited for studying presynaptic [Ca(2+)](i) and PKC activity involved in neurotransmission, both under physiological conditions and under the influence of neuropharmacological agents...
  17. ncbi Sevoflurane inhibits contraction of uterine smooth muscle from pregnant rats similarly to halothane and isoflurane
    Michiaki Yamakage
    Department of Anesthesiology, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan
    Can J Anaesth 49:62-6. 2002
    ..The present study was designed to clarify the direct effects of the volatile anesthetics halothane, isoflurane and sevoflurane on oxytocin-induced uterine smooth muscle contraction from pregnant rats...
  18. ncbi Intracellular signal transduction during gastrin-induced histamine secretion in rat gastric ECL cells
    Robert Zanner
    II Medizinische Klinik und Poliklinik, Germany
    Am J Physiol Cell Physiol 282:C374-82. 2002
    ..Gastrin-induced calcium influx was mediated by dihydropyridine-sensitive calcium channels that appear to be L-type channels activated through a pathway involving activation of PKC...
  19. ncbi Evidence for capacitative and non-capacitative Ca2+ entry pathways coexist in A10 vascular smooth muscle cells
    Jia Guo Zhou
    Department of Pharmacology, Cardiac and Cerebral Vascular Research Center, Zhongshan Medical College, Sun Yat Sen University 74 Zhongshan 2 Rd Guangzhou, Guangdong, 510089, China
    Life Sci 78:1558-63. 2006
    ..Our results suggest that both capacitative and non-capacitative Ca2+ entry pathways are involved in Ca2+ influx induced by activation of alpha1-AR in A10 vascular smooth muscle cells...
  20. ncbi Excitatory and inhibitory actions of isoflurane in bovine chromaffin cells
    Z Xie
    The University of Chicago, Dept of Anesthesia and Critical Care, 5841 S Maryland, MC 4028, Chicago, IL 60637, USA
    J Neurophysiol 96:3042-50. 2006
    ..At the same time, isoflurane suppressed activation of nAChRs, which presumably blocks "sympathetic drive" to the chromaffin cells. These latter results may help explain why isoflurane produces the hypotension observed clinically...
  21. ncbi Mechanisms of AM404-induced [Ca(2+)](i) rise and death in human osteosarcoma cells
    Hong Tai Chang
    Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
    Toxicol Lett 179:53-8. 2008
    ..AM404 caused cytotoxicity which was possibly mediated by apoptosis...
  22. ncbi Elevation of cytosolic calcium level triggers calcium antagonist-induced gingival overgrowth
    Toshimi Hattori
    Department of Dental Pharmacology, Matsumoto Dental University, Hirooka Gohbara, Shiojiri 399 0781, Japan
    Eur J Pharmacol 583:37-9. 2008
    ..These findings confirm that [Ca2+]i elevation induces the proliferation of gingival fibroblasts...
  23. ncbi Activation of store-operated Ca(2+) channels in trabecular meshwork cells
    Elena Abad
    Laboratory of Neurophysiology, Faculty of Medicine, School of Medicine, University of Barcelona, Barcelona, Spain
    Invest Ophthalmol Vis Sci 49:677-86. 2008
    ..Given that trabecular outflow is modified by tissue contractility, the authors characterized SOCs and studied their participation in TM cell contractility...
  24. ncbi Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells
    Xiaoxia Wang
    Am J Physiol Renal Physiol 293:F1768-76. 2007
    ..Using fura 2 ratiometry, we measured intracellular Ca(2+) concentration [Ca(2+)](i) to determine whether sodium nitroprusside (..
  25. ncbi Effects of trans- and cis-resveratrol on Ca2+ handling in A7r5 vascular myocytes
    Manuel Campos-Toimil
    Departamento de Farmacoloxía, Facultade de Farmacia Universidade de Santiago de Compostela Campus Universitario Sur, E 15782, Santiago de Compostela, Spain
    Eur J Pharmacol 577:91-9. 2007
    ..Taken together, these effects may explain, in part, the endothelium-independent vasorelaxant effects of resveratrol in rat aorta...
  26. ncbi Trimetazidine modulates AMPA/kainate receptors in rat vestibular ganglion neurons
    Govindan Dayanithi
    INSERM, U 583, Institut des Neurosciences de Montpellier, Hopital St Eloi, 80 rue Augustin Fliche, F 34091, Montpellier Cedex 5, France
    Eur J Pharmacol 574:8-14. 2007
    ..This may explain the protective role of trimetazidine in the inner ear suggesting an anti-excitotoxic activity...
  27. ncbi Measurements with fluorescent probes in primary neural cultures; improved multiwell techniques
    Avi Ring
    Department of Protection, Norwegian Defence Research Establishment, Kjeller, Norway
    J Pharmacol Toxicol Methods 56:300-7. 2007
    ..CGN, however, require at least 7 days for differentiation and over time cells tend to cluster and loose adherence to the glass substrate. This problem is accentuated in small wells (e.g. 96-well plates)...
  28. ncbi Direct measurement of local raised subplasmalemmal calcium concentrations in growth cones advancing on an N-cadherin substrate
    Neil Chadborn
    Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
    Eur J Neurosci 15:1891-8. 2002
    ..Our results suggest that guidance cues can use extremely local calcium signals to control pathfinding decisions...
  29. pmc The plasma membrane Na+/Ca2+ exchange inhibitor KB-R7943 is also a potent inhibitor of the mitochondrial Ca2+ uniporter
    J Santo-Domingo
    Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Instituto de Biologia y Genetica Molecular, Universidad de Valladolid and Consejo Superior de Investigaciones Cientificas, Valladolid, Spain
    Br J Pharmacol 151:647-54. 2007
    ..We have studied here its effects on mitochondrial Ca(2+) fluxes...
  30. ncbi Relative contribution of the Na(+)/Ca(2+) exchanger, mitochondria and endoplasmic reticulum in the regulation of cytosolic Ca(2+) and catecholamine secretion of bovine adrenal chromaffin cells
    D M Yang
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, China
    J Neurochem 76:210-6. 2001
    ..When Ca(2+) diffuses away from the exocytotic sites, the mitochondrial Ca(2+) uptake becomes the dominant mechanism...
  31. ncbi Cardiomyocyte dysfunction in sucrose-fed rats is associated with insulin resistance
    K Dutta
    College of Osteopathic Medicine, University of New England, Biddeford, Maine 04005, USA
    Diabetes 50:1186-92. 2001
    ..Furthermore, metformin prevented the development of sucrose-induced insulin resistance and the consequent cardiomyocyte dysfunction...
  32. ncbi Changes in cytosolic calcium in response to noxious heat and their relationship to vanilloid receptors in rat dorsal root ganglion neurons
    W Greffrath
    Institute of Physiology and Pathophysiology, Johannes Gutenberg University, D-55099, Mainz, Germany
    Neuroscience 104:539-50. 2001
    ..Heat-sensitive vanilloid receptors are involved in the induction of calcium transients, and calcium is also released from intracellular stores, but the main fraction of calcium passes through voltage-operated calcium channels...
  33. pmc Simultaneous monitoring of three key neuronal functions in primary neuronal cultures
    Gareth John Owen Evans
    Centre for Integrative Physiology, Hugh Robson Building, George Square, University of Edinburgh, Edinburgh EH8 9XD, UK
    J Neurosci Methods 160:197-205. 2007
    ..We propose that this assay of three key neuronal functions could be simply translated into a high content screening format for studies investigating small molecule inhibitors of these processes...
  34. ncbi Extracellular NAD(+) induces calcium signaling and apoptosis in human osteoblastic cells
    M Romanello
    Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, G. Gaslini Institute, Via Licio Giorgieri 1, Trieste, I-34127, Italy
    Biochem Biophys Res Commun 285:1226-31. 2001
    ..The first changes could be observed after 24 h of treatment and became fully evident after 72-96 h. We propose a role of extracellular NAD(+) in bone homeostatic control...
  35. ncbi NPC-14686 (Fmoc-l-homophenylalanine)-induced CaCa2+ increases and death in human prostate cancer cells
    Jong Khing Huang
    Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 813
    Life Sci 78:964-9. 2006
    ..NPC-14686 may be cytotoxic to prostate cancer cells...
  36. ncbi Dendritic calcium encodes striatal neuron output during up-states
    Jason N D Kerr
    Unit of Neural Network Physiology, Laboratory of Systems Neuroscience, National Institute of Mental Health, Bethesda, Maryland 20892, USA
    J Neurosci 22:1499-512. 2002
    ..We suggest that this conveys information about the contribution of a spiny projection neuron to a basal ganglia output specifically back to the corticostriatal synapses involved in generating these outputs...
  37. ncbi An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos
    Greg FitzHarris
    Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
    J Cell Sci 118:4563-75. 2005
    ..Thus, although an induced [Ca2+]i increase can accelerate mitosis entry, neither cytosolic nor perinuclear [Ca2+] increases appear to be necessary for progression through mitosis in mouse embryos...
  38. ncbi Mechanisms of cytosolic Ca2+ suppression by prostaglandin E2 receptors in rat melanotrophs
    T Nagata
    Department of Physiology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan
    J Neuroendocrinol 15:33-41. 2003
    ..We have compared the possible cellular and molecular mechanisms of inhibition by dopamine and PGE2...
  39. ncbi Multiple purinergic receptors lead to intracellular calcium increases in cultured rat Sertoli cells
    W H Ko
    Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Basic Medical Sciences Building, Shatin, Hong Kong, China
    Life Sci 72:1519-35. 2003
    ..Taken together, these data indicate that both metabotropic receptors and ionotropic receptors are present in rat Sertoli cells through which extracellular nucleotides can act...
  40. ncbi Thiol oxidation by 2,2'-dithiodipyridine induced calcium mobilization in MG63 human osteosarcoma cells
    Soong Yu Kuo
    Department of Medical Technology, Fooyin Institute of Technology, Kaohsiung 813, Taiwan
    Life Sci 72:1733-43. 2003
    ..The 2,2'-DTDP-induced store Ca(2+) release appeared to be dependent on oxidation of membranes...
  41. ncbi Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia
    Ronald J Knox
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Neurobiol 60:411-23. 2004
    ..In contrast, two potential store-operated Ca(2+) entry current inhibitors, SKF-96365 and Ni(2+), attenuated NaP-induced Ca(2+) entry. We conclude that NaP activates a slow, persistent Ca(2+) influx in Aplysia bag cell neurons...
  42. ncbi Participation of intracellular Ca2+ stores in arteriolar conducted responses
    Yasuaki Yashiro
    First Department of Physiology, Shinshu University School of Medicine, Matsumoto 390 8621, Japan
    Am J Physiol Heart Circ Physiol 285:H65-73. 2003
    ..cell intracellular Ca2+ concentration ([Ca2+]i) at the site of stimulation, as measured with the Ca2+ indicator fura 2. The Ca2+ pump inhibitor thapsigargin was used to limit filling of Ca2+ stores in smooth muscle and endothelial ..
  43. pmc A study comparing the actions of gabapentin and pregabalin on the electrophysiological properties of cultured DRG neurones from neonatal rats
    David McClelland
    Department of Biomedical Sciences, Institute of Medical Sciences, The University of Aberdeen, Foresterhill, Aberdeen AB25 2RL, Scotland, UK
    BMC Pharmacol 4:14. 2004
    ....
  44. ncbi Effect of NPC-14686 (Fmoc-L-homophenylalanine) on intracellular Ca2+ levels in human hepatoma cells
    Chung Ren Jan
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, 386 Ta Chung 1st Road, Kaohsiung 813, Taiwan
    Life Sci 72:2571-80. 2003
    ....
  45. ncbi Dichlorodiphenylchloroethylene elevates cytosolic calcium concentrations and oscillations in primary cultures of human granulosa-lutein cells
    E V Younglai
    Department of Obstetrics and Gynecology, Reproductive Biology Division, McMaster University, Health Sciences Centre, Hamilton, Ontario, Canada
    Biol Reprod 70:1693-700. 2004
    ..Our data therefore highlight a novel mechanism through which DDE can alter endocrine homeostasis and possibly act as an endocrine toxicant...
  46. ncbi Novel effect of N-palmitoyl-L-serine phosphoric acid on cytosolic Ca2+ levels in human osteoblasts
    Chung Ren Jan
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 813
    Pharmacol Toxicol 93:71-6. 2003
    ..Collectively, L-NASPA rapidly increased [Ca2+]i in MG63 cells by evoking both extracellular Ca2+ influx and intracellular Ca2+ release, and is cytotoxic at higher concentrations...
  47. ncbi Effect of tyrosine kinase blockade on norepinephrine-induced cytosolic calcium response in rat afferent arterioles
    Max Salomonsson
    Department of Medical Physiology, The Panum Institute, University of Copenhagen, Denmark
    Am J Physiol Renal Physiol 286:F866-74. 2004
    ..Ca(2+)](i) was measured in microdissected arterioles using ratiometric photometry of fura 2 fluorescence...
  48. ncbi Mechanisms of direct inhibitory action of isoflurane on vascular smooth muscle of mesenteric resistance arteries
    Takashi Akata
    Department of Anesthesiology and Critical Care Medicine, Kyushu University, Fukuoka, Japan
    Anesthesiology 99:666-77. 2003
    ..Isoflurane has been shown to directly inhibit vascular reactivity. However, less information is available regarding its underlying mechanisms in systemic resistance arteries...
  49. ncbi Effect of the antidepressant maprotiline on calcium movement and the viability of renal tubular cells
    Shu Shong Hsu
    Department of Surgery, Kaohsiung Veterans General Hospital, 813 Kaohsiung, Taiwan
    Arch Toxicol 78:453-9. 2004
    ....
  50. ncbi Changes in intracellular calcium transients and LTP in the surround of visual cortex lesions in rats
    G Barmashenko
    Department of Neurobiology, Ruhr University Bochum, D 44780, Bochum, Germany
    Brain Res 990:120-8. 2003
    ..This mechanism may support synaptic reorganization in the surround of the deafferented region in rat visual cortex...
  51. ncbi Effect of nortriptyline on intracellular Ca2+ handling and proliferation in human osteosarcoma cells
    Shu Shong Hsu
    Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan 813
    Basic Clin Pharmacol Toxicol 95:124-30. 2004
    ..These data suggest that nortriptyline rapidly increases [Ca2+]i in human osteosarcoma cells by stimulating both extracellular Ca2+ influx and intracellular Ca2+ release, and is cytotoxic at high concentrations...
  52. ncbi Regulation of intracellular Ca2+ release in corpus cavernosum smooth muscle: synergism between nitric oxide and cGMP
    Beatrice A Williams
    Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada N6A 5C1
    Am J Physiol Cell Physiol 288:C650-8. 2005
    ..01). Our results demonstrate that NO and cGMP act synergistically to reduce Ca(2+) release from intracellular stores. Reduction of intracellular Ca(2+) release may contribute to relaxation of the corpus cavernosum, leading to erection...
  53. ncbi Amino acids and Ca2+ stimulate different patterns of Ca2+ oscillations through the Ca2+-sensing receptor
    Steven H Young
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, School of Medicine, University of California, Los Angeles, California 90095 1786, USA
    Am J Physiol Cell Physiol 282:C1414-22. 2002
    ..Thus both amino acids and Ca2+, acting through the same CaR, produce oscillatory increases in [Ca2+]i, but the resultant oscillation pattern and frequency allow the cell to discriminate which agonist is bound to the receptor...
  54. ncbi Release from intracellular stores is responsible for calcium signaling with von Willebrand factor in human platelets
    John C Kermode
    Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Thromb Haemost 87:699-705. 2002
    ..Influx across the plasma membrane plays no more than a minor role, probably representing "capacitative entry" to refill the intracellular stores...
  55. ncbi Ionic basis of cold receptors acting as thermostats
    Makoto Okazawa
    Division of Biological Information, Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University, Kyoto 606 8501, Japan
    J Neurosci 22:3994-4001. 2002
    ..Because these thermal properties were consistent with that in cold fibers, we conclude that the same cold receptors exist at nerve endings and generate afferent impulses for cold sensation and heat-gain behaviors in response to cold...
  56. ncbi Functional characterization of calcium channels localized on the growth cones of cultured rat dorsal root ganglion cells
    Hiromitsu Soeda
    Department of Autonomic Physiology, Medical Research Institute, Tokyo Medical and Dental University, 2 3 10 Kandasurugadai, Chiyoda ku, Tokyo 101 0062, Japan
    Neurosci Lett 325:5-8. 2002
    ..It is concluded that the omega - CgTx - as well as omea - aga IVA - sensitive Ca2+ channels are involved in the ([Ca2+](i))-increase in the growth cones of the cultured DRG neurons, leading to glutamate release before synapse formation...
  57. ncbi Membrane potential dependent modulations of calcium oscillations in insulin-secreting INS-1 cells
    M Herbst
    Physiologisches Institut II, Universitat Freiburg, Freiburg, Germany
    Cell Calcium 31:115-26. 2002
    ..These results support the hypothesis that [Ca(2+)](i) oscillations depend on functional voltage-dependent Ca(2+) and K(+) channels and are interrupted by a hyperpolarization in insulin-secreting cells...
  58. ncbi Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga
    Brett Berke
    Interdisciplinary Program in Neuroscience and Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
    J Neurosci 22:4437-47. 2002
    ..Furthermore, rut disrupted the distinction in Ca(2+) responses observed between the periphery and central domain of growth cones with motile filopodia...
  59. ncbi Novel action of lignans isolated from Hernandia nymphaeifolia on Ca(2+) signaling in renal tubular cells
    Yu Ying Chao
    Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
    Eur J Pharmacol 443:31-8. 2002
    ..They caused Ca(2+) influx but reduced thapsigargin-induced capacitative Ca(2+) entry and also thapsigargin- and ATP-induced Ca(2+) release. Additionally, deoxypodophyllotoxin and yatein may be cytotoxic...
  60. ncbi Intracellular ca(2+) pools and fluxes in cardiac muscle-derived h9c2 cells
    Antonio Lax
    Departamento de Bioquimica y Biologia Molecular A, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain
    J Bioenerg Biomembr 37:249-59. 2005
    ..e., adding thapsigargin. These observations indicate that the mitochondria interpret the cytoplasmic Ca(2+) signals generated in the reticular store...
  61. pmc Intracellular and extracellular concentrations of Na+ modulate Mg2+ transport in rat ventricular myocytes
    Michiko Tashiro
    Department of Physiology, Tokyo Medical University, 6 1 1 Shinjuku, Shinjuku ku, Tokyo 160 8402, Japan
    Biophys J 89:3235-47. 2005
    ..These results contribute to a quantitative characterization of the Na+-Mg2+ exchange in cardiac myocytes...
  62. ncbi Gossypol, a component in cottonseed, induced increases in cytosolic Ca2+ levels in Chang liver cells
    J S Cheng
    Department of Medicine, Kaohsiung Veterans General Hospital, 386 Ta Chung 1st Road, Taiwan
    Toxicon 40:851-6. 2002
    ....
  63. ncbi Role of the actin cytoskeleton in store-mediated calcium entry in glioma C6 cells
    Pawelz Sabała
    Laboratory of Signal Transduction, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Str, Warsaw, Poland
    Biochem Biophys Res Commun 296:484-91. 2002
    ..While the agonists evoke a store-mediated Ca2+ entry that is dependent on the actin cytoskeleton, thapsigargin apparently activates an additional mechanism, which is independent of the disruption of the cytoskeleton...
  64. ncbi Effect of desipramine on Ca2+ levels and growth in renal tubular cells
    Chin Man Ho
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan
    Cell Signal 17:837-45. 2005
    ..Together, these findings suggest that desipramine increases baseline [Ca2+]i in renal tubular cells by evoking both extracellular Ca2+ influx and intracellular Ca2+ release, and can cause apoptosis...
  65. ncbi K+-dependent Na+/Ca2+ exchange is a major Ca2+ clearance mechanism in axon terminals of rat neurohypophysis
    Suk Ho Lee
    Department of Physiology and National Research Laboratory for Cellular Signaling, Seoul National University College of Medicine, Chongno Ku, Seoul, 110 799, Korea
    J Neurosci 22:6891-9. 2002
    ..Considering that axon terminals are subject to an invasion by high-frequency Na+ spikes, which may lower Na+ gradients, the presence of NCKX may have a functional significance in intracellular Ca2+ regulation...
  66. ncbi The effects of alfentanil on cytosolic Ca(2+) and contraction in rat ventricular myocytes
    Mark D Graham
    School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom
    Anesth Analg 98:1013-6, table of contents. 2004
    ....
  67. pmc Mitochondria regulate inactivation of L-type Ca2+ channels in rat heart
    J A Sanchez
    Department of Pharmacology, CINVESTAV, AP 14 740, Mexico DF, 07300, Mexico
    J Physiol 536:387-96. 2001
    ....
  68. ncbi Capsazepine elevates intracellular Ca2+ in human osteosarcoma cells, questioning its selectivity as a vanilloid receptor antagonist
    Hsiu Peng Teng
    Department of Orthopaedic Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan
    Life Sci 75:2515-26. 2004
    ..Capsazepine may be mildly cytotoxic...
  69. doi Dynamic imaging of calcium and STIM1 in the same cell using wide-field and TIRF microscopy
    Simon Walker
    The Babraham Institute, Laboratory of Molecular Signalling, Cambridge, UK
    Biotechniques 45:347-8. 2008
  70. pmc Quantitative comparisons of muscarinic and bradykinin receptor-mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells
    G B Willars
    Department of Cell Physiology and Pharmacology, University of Leicester
    Br J Pharmacol 114:1133-42. 1995
    ....
  71. ncbi Extrinsic tension results in FGF-2 release, membrane permeability change, and intracellular Ca++ increase in immature cranial sutures
    J C Yu
    Department of Surgery, Medical College of Georgia, Augusta, USA
    J Craniofac Surg 12:391-8. 2001
    ..These subroutines integrate to generate the emergent property of directed cranial expansion with dissipation of the initiating strains...
  72. pmc Blockade by ifenprodil of high voltage-activated Ca2+ channels in rat and mouse cultured hippocampal pyramidal neurones: comparison with N-methyl-D-aspartate receptor antagonist actions
    J Church
    Department of Anatomy, University of British Columbia, Vancouver, Canada
    Br J Pharmacol 113:499-507. 1994
    ..Keywords: Ifenprodil; voltage-activated calcium channels; N-methyl-D-aspartate (NMDA); polyamines; cultured hippocampal pyramidal neurones..
  73. ncbi Regulation of the human bradykinin B2 receptor expressed in sf21 insect cells: a possible role for tyrosine kinases
    G Reyes-Cruz
    Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico, D F 04510 Mexico
    J Cell Biochem 76:658-73. 2000
    ..In these cells, bradykinin induced a transient increase of intracellular calcium ([Ca(2+)](i)) in fura 2-AM loaded sf21 cells, and promoted [(35)S]-GTP(gamma)S binding to membranes...
  74. ncbi Effect of tacrine on intracellular calcium in cholinergic SN56 neuronal cells
    V Dolezal
    Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska, Prague
    Brain Res 769:219-24. 1997
    ..The finding that low micromolar concentrations of tacrine may interfere with calcium-dependent events is likely to be of importance for the evaluation of the therapeutic potential of the drug...
  75. pmc Characteristics of nucleotide receptors that cause elevation of cytoplasmic calcium in immortalized rat brain endothelial cells (RBE4) and in primary cultures
    M Nobles
    King s College London, Biomedical Sciences Division, Strand
    Br J Pharmacol 115:1245-52. 1995
    ..The implications for physiology are discussed...
  76. ncbi [Cellular adhesion to elements of the extracellular matrix in the hypertensive rat modulates the effect of angiotensin II]
    H Bouillier
    INSERM U337, , Paris
    Arch Mal Coeur Vaiss 92:1053-7. 1999
    ..These modulations of Cai++ mobilization could play a role in the regulation of growth and differentiation of cells during the development of hypertension...
  77. ncbi Inhibitors of Na-K-ATPase in human urine: effects of ouabain-like factors and of vanadium-diascorbate on calcium mobilization in rat vascular smooth muscle cells: comparison with the effects of ouabain, angiotensin II, and arginine-vasopressin
    H Meyer-Lehnert
    Renal Section, Medical Policlinic, University of Bonn, Germany
    Am J Hypertens 13:364-9. 2000
    ..The physiologic and pathologic roles of these and additional OLF in body fluid and blood pressure regulation and in hypertension have yet to be evaluated...
  78. pmc Submaximal stimulation of porcine endothelial cells causes focal Ca2+ elevation beneath the cell membrane
    W F Graier
    Department of Medical Biochemistry, University of Graz, Austria
    J Physiol 506:109-25. 1998
    ..The existence of such a subplasmalemmal Ca2+ control unit (SCCU) needs to be considered in discussions of Ca2+ signalling, especially when cytoplasmic Ca2+ dyes, such as fura-2 or Calcium Green-1, are used...
  79. pmc Reduction of calcium inactivation of sarcoplasmic reticulum calcium release by fura-2 in voltage-clamped cut twitch fibers from frog muscle
    D S Jong
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510 8026
    J Gen Physiol 102:333-70. 1993
    ..On average, 5-6 mM fura-2 increased the quasi-steady rate of release, compared with 0 mM fura-2, by 6.5 and 2.9, respectively, in four fibers from Rana temporaria and three from Rana pipiens.(ABSTRACT TRUNCATED AT 400 WORDS)..
  80. ncbi Fluoxetine inhibits calcium-activated currents of salamander rod photoreceptor somata and presynaptic terminals via modulation of intracellular calcium dynamics
    Ernest C Steele
    Neuroscience Institute, Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA 30310, USA
    Mol Vis 11:1200-10. 2005
    ....
  81. ncbi Effect of hyperosmolarity on agonist-induced increases of intracellular calcium in human platelets
    Oscar A Gende
    Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, 60 y 120, 1900 La Plata, Argentina
    Thromb Res 110:33-7. 2003
    ....
  82. pmc Anti-diabetic biguanides inhibit hormone-induced intracellular Ca2+ concentration oscillations in rat hepatocytes
    J J Ubl
    Pharmakologisches Institut, Universitat Bern, Switzerland
    Biochem J 304:561-7. 1994
    ..This IP3-induced release of Ca2+ from intracellular stores could be diminished by prior addition of metformin and phenformin.(ABSTRACT TRUNCATED AT 400 WORDS)..
  83. pmc Differential effects of the PAF receptor antagonist UK-74,505 on neutrophil and eosinophil accumulation in guinea-pig skin
    M J Sanz
    Department of Applied Pharmacology, National Heart and Lung Institute, London
    Br J Pharmacol 113:513-21. 1994
    ..Keywords: PAF; PAF receptor antagonist; UK-74,505; neutrophil accumulation; eosinophil accumulation; oedema formation;passive cutaneous anaphylactic (PCA) reaction; zymosan; inflammation; guinea-pig skin..
  84. pmc Endothelin-1 and endothelin-3 regulate differently vasoconstrictor responses of smooth muscle of the porcine coronary artery
    M Ushio Fukai
    Division of Molecular Cardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Br J Pharmacol 114:171-9. 1995
    ....
  85. pmc Sensitivity of G-protein involved in endothelin-1-induced Ca2+ influx to pertussis toxin in porcine endothelial cells in situ
    H Aoki
    Division of Molecular Cardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Br J Pharmacol 111:989-96. 1994
    ..The Ca2+ influx is regulated by an IAP-sensitive G-protein, while the release of Ca2+ from the intracellular store is not...
  86. pmc Inhibition of neuronal Ca(2+) influx by gabapentin and subsequent reduction of neurotransmitter release from rat neocortical slices
    K Fink
    Department of Pharmacology and Toxicology, University of Bonn Medical School, Reuterstrasse 2b, 53113 Bonn, Germany
    Br J Pharmacol 130:900-6. 2000
    ..The latter effect leads to a reduced activation of AMPA receptors which contribute to K(+)-evoked noradrenaline release from noradrenergic varicosities, resulting in an indirect inhibition of noradrenaline release...
  87. ncbi Role of the cardiac Na(+)/H(+)exchanger in [Ca(2+)](i)and [Na(+)](i)handling during intracellular acidosis. Effect of cariporide (Hoe 642)
    Aida Salameh
    Medizinische Klinik I, Abt Kardiologie, Universitat Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Pharmacol Res 45:35-41. 2002
    ..The effect of cariporide (Hoe 642) in intracellular acidosis seems to be based on antagonization of the rise in intracellular sodium rather than calcium in this model...
  88. pmc Regulation of secretory granule recruitment and exocytosis at rat neurohypophysial nerve endings
    D R Giovannucci
    Department of Physiology, University of Michigan Medical School, Ann Arbor 48109, USA
    J Physiol 498:735-51. 1997
    ....
  89. ncbi Effect of oxytocin on free intracellular Ca2+ levels and progesterone release by human granulosa-lutein cells
    A Mayerhofer
    Abteilung Anatomie und Zellbiologie, Universitat Ulm, Germany
    J Clin Endocrinol Metab 77:1209-14. 1993
    ..We, therefore, propose that oxytocin may have autocrine and/or paracrine functions in human granulosa-lutein cells, including fine-tuning of progesterone release...
  90. pmc Bromination pattern of hydroxylated metabolites of BDE-47 affects their potency to release calcium from intracellular stores in PC12 cells
    Milou M L Dingemans
    Neurotoxicology Research Group, Toxicology Division, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands
    Environ Health Perspect 118:519-25. 2010
    ..Recently, several other hydroxylated BDE-47 metabolites, besides 6-OH-BDE-47, have been detected in human serum and cord blood...
  91. pmc Acetylcholine and tachykinins involvement in the caffeine-induced biphasic change in intracellular Ca2+ in bovine airway smooth muscle
    Luis M Montaño
    Departamento de Farmacologia, Facultad de Medicina, UNAM, Ciudad Universitaria, CP 04510, Mexico DF, Mexico
    Br J Pharmacol 139:1203-11. 2003
    ..69 vs. 1.97 pg ml(-1) mg tissue(-1), respectively, P=0.053). 5. We concluded that acetylcholine and tachykinins release are involved in the caffeine-induced biphasic changes in cytosolic Ca(2+) concentration...
  92. pmc Human conjunctival epithelial cell responses to platelet-activating factor (PAF): signal transduction and release of proinflammatory cytokines
    Najam A Sharif
    Pharmaceutical Products Research, Core Pharmacology and Imaging, Alcon Research Ltd, Fort Worth, TX 76134, USA
    Mol Vis 15:1153-61. 2009
    ....
  93. pmc Multiple effects of ryanodine on intracellular free Ca2+ in smooth muscle cells from bovine and porcine coronary artery: modulation of sarcoplasmic reticulum function
    C Wagner Mann
    Department of Physiology, School of Medicine, University of Missouri, Columbia 65211
    Br J Pharmacol 105:903-11. 1992
    ....
  94. pmc Caffeine-evoked, calcium-sensitive membrane currents in rabbit aortic endothelial cells
    J Rusko
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, FL 33101, USA
    Br J Pharmacol 115:133-41. 1995
    ..The ionic dependence and inhibition of the caffeine sensitive current by TEA and Zn2+ suggests that Ca2+-activated K+ and Cl- conductances contribute to the caffeine response in rabbit aortic endothelial cells...
  95. ncbi Effects of beta-escin and saponin on the transverse-tubular system and sarcoplasmic reticulum membranes of rat and toad skeletal muscle
    B S Launikonis
    Department of Zoology, La Trobe University, Plenty Rd, Bundoora 3083, Victoria, Australia
    Pflugers Arch 437:955-65. 1999
    ....
  96. ncbi Protein kinase C modulator effects on parathyroid hormone-induced intracellular calcium and morphologic changes in UMR 106-H5 osteoblastic cells
    M Babich
    University of Illinois College of Medicine, Rockford, Illinois 61107 1897, USA
    J Cell Biochem 65:276-85. 1997
    ..The data suggest PKC contributes to UMR 106-H5 cell morphology and selectively regulates signal pathways activated by PTH to promote either cell contraction (cAMP) or extension (1,4,5-IP3/Cai2+)...
  97. pmc The mechanisms of the relaxation induced by vasoactive intestinal peptide in the porcine coronary artery
    J Kawasaki
    Division of Molecular Cardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Br J Pharmacol 121:977-85. 1997
    ....
  98. pmc Relationship between intracellular calcium and its muffling measured by calcium iontophoresis in snail neurones
    C J Schwiening
    Department of Physiology, School of Medical Sciences, University of Bristol, UK
    J Physiol 491:621-33. 1996
    ..However, the variability among neurones in intracellular calcium muffling power was large enough to obscure the additional buffering produced by fura-2 in pooled experiments...
  99. pmc Selective deletion of the alpha5 subunit differentially affects somatic-dendritic versus axonally targeted nicotinic ACh receptors in mouse
    Harald Fischer
    Division of Biochemistry and Molecular Biology, Centre for Brain Research, Medical University Vienna, Spitalgasse 4, A 1090 Vienna, Austria
    J Physiol 563:119-37. 2005
    ..These results confirm and significantly extend our previous observation that in the sympathetic nervous system, somatic and prejunctional receptors are different and rely on the presence of the alpha5 subunit in a distinct manner...
  100. ncbi Mode of antinociceptive and toxic action of alkaloids of Aconitum spec
    U T Gutser
    Institut für Naturheilkunde, Universitatsklinikum Ulm, Germany
    Naunyn Schmiedebergs Arch Pharmacol 357:39-48. 1998
    ..The structure/activity relationship shows that alkaloids that activate or block Na+ channels have a benzoyl ester side chain in the C-14 or C-4 positions respectively, whereas the other compounds lack this group...
  101. pmc Cytosolic calcium concentration-force relation during contractions in the rabbit femoral artery: time-dependency and stimulus specificity
    Y Fukuizumi
    Division of Molecular Cardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Br J Pharmacol 114:329-38. 1995
    ..Based on the above findings we suggest the presence of a time-dependent and stimulus-specific modulation of the Ca2+ sensitivity in the contractile apparatus of arterial smooth muscles...

Research Grants62

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Imbalances in Calcium Homeostasis: Mechanism Behind Ethanol-Related Dendritic Deg
    Cynthia A Dlugos; Fiscal Year: 2010
    ..Permanent deficits result from these alterations that become more prevalent with age and predispose our increasing elderly population to falls, accidents, and cognitive dysfunctions. ..
  3. Flow-induced coronary vasospasm in diabetic patients
    Zsolt Bagi; Fiscal Year: 2013
    ..Data obtained in this project will also help to develop novel avenues for effective therapeutic strategies, such as the use of prostanoid inhibitors at the time of PCI, to prevent no reflow in patients with T2-DM. ..
  4. Ouabain, Na+ Pumps, and Ca2+ Signaling in Human Arteries
    Laura K Antos; Fiscal Year: 2010
    ..I anticipate that the results will provide the first direct evidence that the underlying key molecular mechanisms in salt-dependent hypertension in rodents function similarly in humans. ..
  5. LISTERIA AND SHIGELLA USE ACTIN TO SPREAD CELL TO CELL
    Frederick Southwick; Fiscal Year: 2013
    ..These discoveries can be applied to all forms of cell motility, including tumor metastasis, neurite outgrowth, and platelet spreading. ..
  6. Modulation of calcium signaling by changes in STIM expression
    Jonathan Soboloff; Fiscal Year: 2013
    ..Considered in combination, these investigations will provide the framework for a new understanding of how Ca2+ signals in cells are modulated over extended time periods via crosstalk between autocrine, paracrine and endocrine factors. ..
  7. ACTIVITY OF BRAINSTEM NEURONS
    Nae J Dun; Fiscal Year: 2010
    ..glutamate and cocaine before and after treatment of sigma-1 receptor antagonists will be measured by means of the Fura 2 method...
  8. Mechanism of Desensitization of Perivascular Nerve Ca2+-sensing Receptor Signalin
    EMMANUEL MODESTO AWUMEY; Fiscal Year: 2013
    ..In addition, the proposed studies will provide training opportunities for under-represented minority students at NCCU and prepare them for future careers in biomedical research. ..
  9. Mechanisms of Endothelial Cell Hyperpolarization
    Sean P Marrelli; Fiscal Year: 2012
    ..influx in response to ATP in freshly isolated middle cerebral artery (MCA) ECs and in the ECs of pressurized MCA (Fura 2 dye), and demonstrate the role of the identified TRP channels by pharmacological and RNA silencing techniques (..
  10. Olympus BX-DSU Live-Cell Disk Scanning Imaging / Electrophysiology System
    Bradley K Taylor; Fiscal Year: 2010
    ....
  11. Characterization of D3cpv cameleon sensitivity to zinc
    Lech Kiedrowski; Fiscal Year: 2012
    ..These experiments will determine whether using D3cpv, one can resolve fura-2FF signal ambiguities. ..
  12. Functions of GABAergic interneurons in the piriform cortex
    DAVID COFFEY SHERIDAN; Fiscal Year: 2010
    ..This information may be useful in developing new drugs and therapies for sensory and neurological disorders, diseases such as Alzheimer's and Parkinson's diseases that result in olfactory deficits. ..
  13. PH AND MYOCARDIAL CONTRACTILITY
    Donghee Kim; Fiscal Year: 1992
    ..These studies are expected to provide important additional knowledge on the effect of pH on Ca homeostasis and the underlying mechanisms involved in acidosis- or alkalosis-mediated changes in myocardial contractility...
  14. CALCIUM REGULATION IN ARTERIAL MYOCYTES
    William Goldman; Fiscal Year: 1993
    ..Moreover, these findings may be useful in elucidating pathophysiological processes such as those associated with hypertension...
  15. INTEGRIN AVB3 SIGNALLING IN LUNG ENDOTHELIUM
    Sunita Bhattacharya; Fiscal Year: 1999
    ..No previous understanding of the role of tyrosine phosphorylation in endothelial permeability regulation exists. This proposed studies are therefore outstandingly novel. ..
  16. VASCULAR ENDOTHELIUM AND FLUID SHEAR STRESS IN VITRO
    C Dewey; Fiscal Year: 1990
    ..Such information would provide new insights into vascular pathology and the pathogenesis of vascular diseases such as atherosclerosis and thrombosis...
  17. CELLULAR MECHANISMS IN ATHEROGENESIS
    COLIN SCHWARTZ; Fiscal Year: 1992
    ..are examined with emphasis on EC membrane fluidity (DPH), phosphoinositide turnover, Ca++ mobilization (Fura 2), and cytoskeletal function...
  18. ABNORMALITIES OF PLATELET CALCIUM IN DISEASE
    J Ware; Fiscal Year: 1991
    ..Finally, these studies could provide the basis for the use of measurements of intracellular second messengers in clinical medicine...
  19. SYNAPTIC TRANSMISSION IN THE RETINA
    Stephen Yazulla; Fiscal Year: 2006
    ..synaptic ribbons serve as a calcium store for transmitter release. As Parkinson's is a disease of CNS dopamine, insight also will be obtained on the function of dopamine-compromised retinas. [unreadable] [unreadable]..
  20. DEVELOPING HEART--BIOPHYSICAL ASPECTS
    Page Anderson; Fiscal Year: 1999
    ....
  21. HYPERTENSION MECHANISMS AND VASCULAR ION EXCHANGE
    Allan Jones; Fiscal Year: 2000
    ..Moreover, this model which appears to be critically dependent on SBB will allow us to test concepts that have wide applicability to other cell systems which have significant interactions between plasma and SR membranes. ..
  22. EXCITATION-CONTRACTION COUPLING IN VENTRICULAR CELLS
    Mark Cannell; Fiscal Year: 1990
    ..Such information should prove useful for the future development of new inotropic agents and provide a sound scientific basis for the understanding of cardiac failure...
  23. MECHANISMS OF GLUTAMATE-MEDIATED C-FOS EXPRESSION
    Steven Finkbeiner; Fiscal Year: 1999
    ..Elucidating mechanisms of glutamate induced gene transcription and plasticity may lead to new treatments for diseases associated with cellular hyperexcitability and glutamate toxicity. ..
  24. ADRENERGIC FACTORS AND ARRHYTHMOGENIC METABOLITES
    Peter Corr; Fiscal Year: 1992
    ....
  25. Functional Analysis of A Novel Prostate-Associated Gene
    Yi Lu; Fiscal Year: 2005
    ..The long-term objectives are (1) to elucidate a novel apoptotic signal transduction pathway; (2) to determine whether hpHyde has the potential to be used as a biomarker in prostate cancer diagnosis and prognosis. ..
  26. DEVELOPMENT OF CHLORIDE-SENSITIVE FLUORESCENT INDICATORS
    Alan Verkman; Fiscal Year: 1991
    ..The availability of a good fluorescent Cl indicator should have wide applications in transport physiology and facilitate the understanding of Cl transport mechanisms and Cl regulation in both epithelial and non-epithelial membranes...
  27. MEDIATORS, AIRWAY SMOOTH MUSCLE GROWTH AND CONTRACTILITY
    Michael Grunstein; Fiscal Year: 1992
    ....
  28. CADMIUM AND OVARIECTOMY--INDUCED LOSS OF BONE MINERAL
    MARYKA BHATTACHARYYA; Fiscal Year: 2000
    ..The proposed research will address these important issues. ..
  29. CONTROL OF CALCIUM IN AIRWAY SMOOTH MUSCLE CELLS
    Hiroshi Yamaguchi; Fiscal Year: 1992
    ....
  30. GLIAL NEURONAL INTERACTIONS IN NEURODEGENERATION
    BRUCE KRUEGER; Fiscal Year: 2000
    ..Deficits of this kind would be expedited to make cells vulnerable to toxic influences that accumulate with aging. Such vulnerability may play a role in the development of neurodegenerate disorders. ..
  31. CARRIER-MEDIATED O2 TRANSPORT IN ISOLATED HEART CELLS
    Beatrice Wittenberg; Fiscal Year: 1991
    ..We will seek to differentiate whether gap junctional conductance is affected either by decreased total ATP production or by subsequent changes in cellular ionic gradients...
  32. PHYSIOLOGY OF ISOLATED HUMAN TASTE RECEPTOR CELLS
    Joseph Brand; Fiscal Year: 2000
    ..Imaging studies of intracellular calcium and membrane potential using the dyes Fura 2 & di-8-ANEPPS will permit basic characterization studies to be performed...
  33. DEVELOPMENTAL ROLE OF G PROTEINS IN NEURONAL MIGRATION
    PHILIP COPENHAVER; Fiscal Year: 2002
    ..Time lapse imaging of individual EP cells injected with Ca Green Dextran or Fura 2 will be used to monitor changes in intracellular Ca with respect to normal migration...
  34. IONIC CURRENTS AND (CA++)I IN CORONARY SMOOTH MUSCLE
    James Johns; Fiscal Year: 1992
    ..It is hoped that this proposed research will enhance the understanding of these important areas...
  35. CALCIUM AND THE PATHOPHYSIOLOGY OF THE ADULT HEART CELL
    ROBERT HAWORTH; Fiscal Year: 2001
    ....
  36. DEVELOPMENT&MODULATION OF CALCIUM CURRENTS IN NEOCORTEX
    Robert Foehring; Fiscal Year: 2003
    ..These studies will contribute to understanding essential cortical functions such as attention, learning, and memory as well as basic mechanisms of diseases such as epilepsy, anxiety and depression. ..
  37. A new method to measure taste bud function
    Stephen Roper; Fiscal Year: 2005
    ..expressing high affinity receptors for candidate neurotransmitters and loaded with the calcium-sensitive dye, Fura 2. The specific aims include investigating whether taste stimulation elicits release of serotonin, norepinephrine, ..
  38. The Role of Calcyon in Synaptic Integration
    Clare Bergson; Fiscal Year: 2004
    ..The results of this research should provide insight into clinical strategies to selectively boost or dampen D1 receptor mediated neuromodulation of excitatory transmission. ..
  39. MEDIATORS OF CARDIAC INJURY
    JOSHUA GOLDHABER; Fiscal Year: 2000
    ....
  40. RADIOMETRIC FLUOROMETER FOR TISSUE ION MEASUREMENTS
    Desmond Radnoti; Fiscal Year: 1993
    ..The fluorometer will be demonstrated in both smooth and cardiac muscle preparations, which have temporal cation responses that span the range of multicellular preparations...
  41. MECHANISM AND FUNCTION OF THE VASCULAR MYOGENIC RESPONSE
    Michael Davis; Fiscal Year: 2002
    ....
  42. CALCIUM REGULATION IN THE DIABETIC HEART
    Amy Davidoff; Fiscal Year: 2001
    ..The adult myocyte culture system provides a well-defined method from elucidating fundamental mechanisms of high glucose that may contribute to the development of myocyte dysfunction in diabetes. ..
  43. A Molecular Dynamics Approach to GnRH Pulsatility
    FREDRIC BOOCKFOR; Fiscal Year: 2006
    ..These principles may be invaluable in devising future therapeutic strategies that can be used to correct reproductive disfunction resulting from abnormal GnRH release. [unreadable] [unreadable]..
  44. Mechanisms of uterine vascular adaptation in pregnancy
    Natalia Gokina; Fiscal Year: 2006
    ..abstract_text> ..
  45. IGF-1, Oxidative Stress and Cardiovascular Aging
    Jun Ren; Fiscal Year: 2002
    ..Our long-term goal is to establish the causal link among IGF-1 deficiency, enhanced oxidative damage and ventricular dysfunction in the progression of cardiovascular aging so that hormonal or antioxidant therapy can be optimized...
  46. Altered Glial Calcium Signaling in Neurodegeneration
    Vera Golovina; Fiscal Year: 2008
    ..Expression of S100beta and beta-APP will be identified by immunoblotting. Determining the mechanisms of IL-1beta - and A-beta-induced Ca2+influx in astrocytes could lead to novel therapeutic strategies for AD. ..
  47. Role of Acetaldehyde in Alcoholic Cardiomyopathy
    Jun Ren; Fiscal Year: 2008
    ..Our long-term goal is to establish the toxic mechanism of acetaldehyde in the development of alcoholic cardiomyopathy so that prevention and treatment can be optimized [unreadable] [unreadable]..
  48. Regulation of the Cardiac Na/Ca Exchanger
    John Reeves; Fiscal Year: 2009
    ..Transfected CHO cells will be used in Aims 1-4. Digital imaging techniques using fluorescent Ca probes will be used to measureNCX transport activity. ..
  49. Hypocretin Neurons
    Anthony N van den Pol; Fiscal Year: 2010
    ..Understanding the role of the endogenous opioid in hypocretin cells should also facilitate the understanding and treatment of narcolepsy. ..
  50. IL-6 Regulation of Hippocampal Synaptic Function
    Donna L Gruol; Fiscal Year: 2010
    ..The proposed studies focus on this issue as it relates to CNS disorders. ..
  51. Regulation of vascular smooth muscle calcium sensitivity
    Paul H Ratz; Fiscal Year: 2010
    ..The Specific Aim of this study will be to test the hypothesis that ROK and PKCzeta both mediate KCI- induced Ca sensitization of FA, and that iPLA2 and PI3K are required as upstream activators of ROK and PKCzeta. ..
  52. EDHF in the Cerebral Circulation
    ROBERT BRYAN; Fiscal Year: 2009
    ..Furthermore, we anticipate that new information regarding tandem pore domain potassium channels will be a big step in the understanding of cerebral circulatory control. ..
  53. NPY DEPRESSION OF HIPPOCAMPAL EPILEPSY
    Anthony van den Pol; Fiscal Year: 2002
    ..NPY is potentially of great interest because its primary action in the normal hippocampus appears to be one of depressing hyperexcited neurons, without a substantial effect on normal neurotransmission. ..
  54. NEURONAL ACTIONS OF HOST FACTORS IN NEUROAIDS
    DONNA GRUOL; Fiscal Year: 2005
    ..The overall design will address effects of both acute and chronic chemokine exposure on neuronal cell function and will include examination of two distinct classes of neurons, excitatory glutamatergic and inhibitory GABAergic neurons. ..
  55. CILIARY FUNCTION AND DIFFERENTIATION OF AIRWAY EPITHELIA
    Matthias Salathe; Fiscal Year: 2005
    ..Furthermore, the studies using microarrays will generate new information on specific signaling pathways and may well serve to identify components of regulatory pathways that were unknown. ..
  56. 2005 Cilia, Mucus, and Mucociliary Interactions GRC
    Matthias Salathe; Fiscal Year: 2005
    ..Finally, poster sessions will complement the plenary sessions and free afternoons will further enable idea exchanges between scientists. ..
  57. CYTOKINE/CHEMOKINE ACTIONS ON DEVELOPING CNS NEURONS
    DONNA GRUOL; Fiscal Year: 2002
    ....
  58. REGULATION OF DETRUSOR SMOOTH MUSCLE CONTRACTION
    PAUL RATZ; Fiscal Year: 2005
    ..Collectively, these studies will provide new insights into the cellular mechanisms regulating DSM contractions. ..
  59. Somatic Ca2+ Signaling in CNS Neurons
    DONNA GRUOL; Fiscal Year: 2007
    ..However, the general principles identified by the studies will also contribute significantly to our understanding of the role of activity-dependent gene expression during development in other CNS neuronal types. [unreadable] [unreadable]..
  60. GABA: EXCITATORY TRANSMITTER IN DEVELOPING HYPOTHALAMUS
    Anthony van den Pol; Fiscal Year: 2008
    ..GABA's excitatory actions during development are widespread throughout the brain. Thus, what we learn from our experiments on hypothalamic neurons should have general applicability to other CNS neurons. ..
  61. AGE RELATED CARDIAC SENSITIVITY TO VOLATILE ANESTHETICS
    Y S Prakash; Fiscal Year: 2001
    ....