glycosylphosphatidylinositols

Summary

Summary: Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They act to anchor proteins or polysaccharides to cell membranes.

Top Publications

  1. ncbi Immunological reactions in response to apicomplexan glycosylphosphatidylinositols
    Françoise Debierre-Grockiego
    UMR Université INRA 0483, Immunologie Parasitaire Vaccinologie et Biothérapies anti infectieuses, UFR Sciences Pharmaceutiques, 31 Avenue Monge, 37200 Tours, France
    Glycobiology 20:801-11. 2010
  2. pmc Effect of glycans and the glycophosphatidylinositol anchor on strain dependent conformations of scrapie prion protein: improved purifications and infrared spectra
    Gerald S Baron
    Laboratory of Persistent Viral Diseases, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Biochemistry 50:4479-90. 2011
  3. pmc The glycosylphosphatidylinositol anchor: a complex membrane-anchoring structure for proteins
    Margot G Paulick
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Biochemistry 47:6991-7000. 2008
  4. ncbi Deficiency of the GPI anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria
    J Takeda
    Department of Immunoregulation, Osaka University, Japan
    Cell 73:703-11. 1993
  5. ncbi The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
    M A Ferguson
    Division of Molecular Parasitology and Biological Chemistry, Department of Biochemistry, The Wellcome Trust Building, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 112:2799-809. 1999
  6. ncbi Induction of proinflammatory responses in macrophages by the glycosylphosphatidylinositols of Plasmodium falciparum: cell signaling receptors, glycosylphosphatidylinositol (GPI) structural requirement, and regulation of GPI activity
    Gowdahalli Krishnegowda
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 0850, USA
    J Biol Chem 280:8606-16. 2005
  7. ncbi Genome-wide identification of fungal GPI proteins
    Piet W J De Groot
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    Yeast 20:781-96. 2003
  8. pmc Rapid cycling of lipid raft markers between the cell surface and Golgi complex
    B J Nichols
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    J Cell Biol 153:529-41. 2001
  9. ncbi Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria
    K Kawagoe
    Department of Immunoregulation, Osaka University, Japan
    Blood 87:3600-6. 1996
  10. ncbi Thematic review series: lipid posttranslational modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids
    Peter Orlean
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Lipid Res 48:993-1011. 2007

Detail Information

Publications358 found, 100 shown here

  1. ncbi Immunological reactions in response to apicomplexan glycosylphosphatidylinositols
    Françoise Debierre-Grockiego
    UMR Université INRA 0483, Immunologie Parasitaire Vaccinologie et Biothérapies anti infectieuses, UFR Sciences Pharmaceutiques, 31 Avenue Monge, 37200 Tours, France
    Glycobiology 20:801-11. 2010
    ..This review describes the biosynthesis of the apicomplexan GPIs and their role in the activation of the host immune system...
  2. pmc Effect of glycans and the glycophosphatidylinositol anchor on strain dependent conformations of scrapie prion protein: improved purifications and infrared spectra
    Gerald S Baron
    Laboratory of Persistent Viral Diseases, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Biochemistry 50:4479-90. 2011
    ..Our IR data provide evidence that similar core structures also comprise wild-type PrP(res)...
  3. pmc The glycosylphosphatidylinositol anchor: a complex membrane-anchoring structure for proteins
    Margot G Paulick
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Biochemistry 47:6991-7000. 2008
    ..We specifically highlight studies in which chemically synthesized GPI anchors and analogues have been employed to study the roles of this unique posttranslational modification...
  4. ncbi Deficiency of the GPI anchor caused by a somatic mutation of the PIG-A gene in paroxysmal nocturnal hemoglobinuria
    J Takeda
    Department of Immunoregulation, Osaka University, Japan
    Cell 73:703-11. 1993
    ..We localized PIG-A to the X chromosome, which accounts for expression of the recessive phenotype of the somatic mutation and the fact that the same one of the multiple biosynthetic steps is affected in all patients so far characterized...
  5. ncbi The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
    M A Ferguson
    Division of Molecular Parasitology and Biological Chemistry, Department of Biochemistry, The Wellcome Trust Building, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 112:2799-809. 1999
    ..Apart from providing stable membrane anchorage, GPI anchors have been implicated in the sequestration of GPI-anchored proteins into specialised membrane microdomains, known as lipid rafts, and in signal transduction events...
  6. ncbi Induction of proinflammatory responses in macrophages by the glycosylphosphatidylinositols of Plasmodium falciparum: cell signaling receptors, glycosylphosphatidylinositol (GPI) structural requirement, and regulation of GPI activity
    Gowdahalli Krishnegowda
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033 0850, USA
    J Biol Chem 280:8606-16. 2005
    ..These results imply that macrophage surface phospholipases play important roles in the GPI-induced innate immune responses and malaria pathogenesis...
  7. ncbi Genome-wide identification of fungal GPI proteins
    Piet W J De Groot
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    Yeast 20:781-96. 2003
    ..pombe and N. crassa are homologous to known or putative GPI proteins from other fungi. We conclude that our algorithm is selective and can also be used for GPI protein identification in other fungi...
  8. pmc Rapid cycling of lipid raft markers between the cell surface and Golgi complex
    B J Nichols
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    J Cell Biol 153:529-41. 2001
    ..The PM-Golgi cycling pathway followed by GPI-GFP could serve to regulate lipid raft distribution and function within cells...
  9. ncbi Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria
    K Kawagoe
    Department of Immunoregulation, Osaka University, Japan
    Blood 87:3600-6. 1996
    ....
  10. ncbi Thematic review series: lipid posttranslational modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids
    Peter Orlean
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Lipid Res 48:993-1011. 2007
    ..GPI biosynthesis is essential for viability in yeast and for embryonic development in mammals. In this review, we describe the biosynthesis of mammalian and yeast GPIs, their transfer to protein, and their subsequent processing...
  11. ncbi Regulation of integrin function by the urokinase receptor
    Y Wei
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 273:1551-5. 1996
    ..These data provide a paradigm for regulation of integrins in which a nonintegrin membrane receptor interacts with and modifies the function of activated integrins...
  12. ncbi Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress
    Taroh Kinoshita
    WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
    J Biochem 144:287-94. 2008
    ..It is thought that association with membrane rafts is important for GPI-APs in signal transduction and other functions. Here, we review recent progress in studies on biosynthesis, remodelling and functions of mammalian GPI-APs...
  13. ncbi Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall
    I Mouyna
    Institut Pasteur, Laboratoire des Aspergillus, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France
    J Biol Chem 275:14882-9. 2000
    ..Our results show that transglycosidases anchored to the plasma membrane via glycosylphosphatidylinositols can play an active role in fungal cell wall synthesis.
  14. pmc Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer
    A K Kenworthy
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Cell Biol 142:69-84. 1998
    ..These findings constrain current models for lipid rafts and the membrane organization of GPI-anchored proteins...
  15. pmc Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem J 339:185-92. 1999
    ..The disruption of the gene caused lethality in S. cerevisiae. These results indicate that GlcNAc-PI de-N-acetylase is conserved between mammals and yeasts and that the de-N-acetylation step is also indispensable in yeasts...
  16. pmc Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells
    J Guignot
    Institut National de la Sante et de la Recherche Medicale, Unité 510, Faculte de Pharmacie Paris XI, F 92296 Chatenay Malabry, France
    Infect Immun 68:3554-63. 2000
    ....
  17. pmc The COBRA family of putative GPI-anchored proteins in Arabidopsis. A new fellowship in expansion
    Francois Roudier
    Department of Biology, New York University, New York, NY 10003, USA
    Plant Physiol 130:538-48. 2002
    ..Together, these results indicate that COB family members are likely to be important new players at the plasma membrane-cell wall interface...
  18. pmc Proinflammatory responses by glycosylphosphatidylinositols (GPIs) of Plasmodium falciparum are mainly mediated through the recognition of TLR2/TLR1
    Jianzhong Zhu
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Exp Parasitol 128:205-11. 2011
    The glycosylphosphatidylinositols (GPIs) of Plasmodium falciparum have been shown to activate macrophages and produce inflammatory responses...
  19. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
    ..These results suggest that the stabilities of other glycosylphosphatidylinositol pathway enzymes are not dependent on GlcNAc-PI de-N-acetylase levels...
  20. pmc beta(1-3)Glucanosyltransferase Gel4p is essential for Aspergillus fumigatus
    Amandine Gastebois
    Unite des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Eukaryot Cell 9:1294-8. 2010
    ..Like the other Gelps, it encodes a glycosylphosphatidylinositol (GPI)-anchored protein. In contrast to the other beta(1-3)glucanosyltransferases analyzed to date, it is essential for this fungal species...
  21. ncbi Glycosylphosphatidylinositol (GPI) anchor is required in Aspergillus fumigatus for morphogenesis and virulence
    Hong Li
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, and The General Hospital of Chinese People s Armed Police Forces, Beijing 100080, China
    Mol Microbiol 64:1014-27. 2007
    ..In vivo assays revealed that the mutant exhibited a reduced virulence in immunocompromised mice. The GPI anchor was not essential for viability, but required for the cell wall integrity, morphogenesis and virulence in A. fumigatus...
  22. ncbi Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum
    Paul R Gilson
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Mol Cell Proteomics 5:1286-99. 2006
    ..Together this work identified 11 and predicted a further 19 GPI-anchored proteins in P. falciparum...
  23. ncbi Selective export of human GPI-anchored proteins from the endoplasmic reticulum
    Carine Bonnon
    Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    J Cell Sci 123:1705-15. 2010
    ..The results suggest that the 24p-23p complex acts as a cargo receptor for GPI-anchored proteins by facilitating their export from the ER in a Sec24-isoform-selective manner involving lipid rafts as early sorting platforms...
  24. ncbi Activation of TLR2 and TLR4 by glycosylphosphatidylinositols derived from Toxoplasma gondii
    Françoise Debierre-Grockiego
    Institut fur Virologie, AG Parasitologie, Philipps University, Marburg, Germany
    J Immunol 179:1129-37. 2007
    ..gondii infection through their activation by the GPIs and could work together with other MyD88-dependent receptors, like other TLRs or even IL-18R or IL-1R, to obtain an effective host response against T. gondii infection...
  25. ncbi GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi
    Morihisa Fujita
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Cell 139:352-65. 2009
    ..Our data demonstrate that GPI glycan acts as an ER-exit signal and suggest that glycan remodeling mediated by PGAP5 regulates GPI-AP transport in the early secretory pathway...
  26. pmc PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins
    Yuko Tashima
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 17:1410-20. 2006
    ..These results demonstrate that PGAP2 is involved in the processing of GPI-APs required for their stable expression at the cell surface...
  27. ncbi Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins
    Birgit Eisenhaber
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Republic Austria
    Bioessays 25:367-85. 2003
    ..This structural proposal would explain the paradoxical properties of the GPI lipid anchor signal motif and of PIG-K/GPI8 orthologs without membrane insertion regions in some species...
  28. ncbi The cell wall architecture of Candida albicans wild-type cells and cell wall-defective mutants
    J C Kapteyn
    Swammerdam Institute of Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
    Mol Microbiol 35:601-11. 2000
    ..albicans follows the same basic rules as S. cerevisiae in constructing a cell wall and indicate that a cell wall salvage mechanism is activated when Candida cells are confronted with cell wall weakening...
  29. ncbi Concentration of GPI-anchored proteins upon ER exit in yeast
    Guillaume A Castillon
    Department of Biochemistry, University of Geneva, Sciences II, 30 Quai Ernest Ansermet, CH 1211 Geneva, Switzerland
    Traffic 10:186-200. 2009
    ..Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p...
  30. ncbi Proinflammatory activity of glycosylphosphatidylinositol anchors derived from Trypanosoma cruzi: structural and functional analyses
    I C Almeida
    Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil
    J Leukoc Biol 70:467-77. 2001
    ..cruzi-derived GPI anchors, which lead to the synthesis of proinflammatory cytokines in macrophages. Finally, we discuss the implications of these findings in resistance and pathogenesis during the infection with T. cruzi...
  31. ncbi Cytoplasmic targeting signals in transmembrane invariant surface glycoproteins of trypanosomes
    Wei Lien Chung
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Biol Chem 279:54887-95. 2004
    ..Taken together, these data demonstrate the presence of a lysine-dependent endocytosis signal in the ISG65 family...
  32. ncbi A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans, Candida albicans, Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombe
    Birgit Eisenhaber
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    J Mol Biol 337:243-53. 2004
    ..Lists of potentially GPI lipid anchored proteins for five fungal proteomes have been generated and the hits have been functionally classified. The fungal big-Pi prediction WWW server as well as precursor lists are available at..
  33. pmc PSSA-2, a membrane-spanning phosphoprotein of Trypanosoma brucei, is required for efficient maturation of infection
    Cristina M Fragoso
    Institut fur Zellbiologie, Universitat Bern, Bern, Switzerland
    PLoS ONE 4:e7074. 2009
    ....
  34. pmc PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins
    Morihisa Fujita
    Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8566, Japan
    Mol Biol Cell 17:5253-64. 2006
    ....
  35. pmc A set of glycosylphosphatidyl inositol-anchored membrane proteins of Plasmodium falciparum is refractory to genetic deletion
    Paul R Sanders
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Infect Immun 74:4330-8. 2006
    ..No distinguishable growth rate or invasion pathway phenotype was detected for the msp-5 knockout line, although its presence as a surface-localized protein was confirmed...
  36. pmc High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes
    A K Kenworthy
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 11:1645-55. 2000
    ..We conclude that in the plasma membrane, lipid rafts either exist only as transiently stabilized structures or, if stable, comprise at most a minor fraction of the cell surface...
  37. pmc The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 16:5236-46. 2005
    ..These two proteins are translated from one mRNA by leaky scanning of the PreY initiation site...
  38. ncbi Synthetic GPI as a candidate anti-toxic vaccine in a model of malaria
    Louis Schofield
    Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia
    Nature 418:785-9. 2002
    ..GPI may contribute to pathogenesis and fatalities in humans. Synthetic GPI is therefore a prototype carbohydrate anti-toxic vaccine against malaria...
  39. ncbi Cellular trafficking of the glypican Dally-like is required for full-strength Hedgehog signaling and wingless transcytosis
    Armel Gallet
    Institut Biologie du Développement et Cancer IBDC, Universite de Nice Sophia Antipolis, UMR 6543 CNRS, Centre de Biochimie, Parc Valrose, 06108 Nice Cedex 2, France
    Dev Cell 14:712-25. 2008
    ..Thus, Dlp endocytosis is a common regulatory mechanism of both Hh and Wg morphogen action...
  40. ncbi Association of glycosylphosphatidylinositol-anchored protein with retroviral particles
    Christoph Metzner
    Institute of Virology, University of Veterinary Medicine, Veterinarplatz 1, A 1210 Vienna, Austria
    FASEB J 22:2734-9. 2008
    ....
  41. pmc Prevalence and boosting of antibodies to Plasmodium falciparum glycosylphosphatidylinositols and evaluation of their association with protection from mild and severe clinical malaria
    J Brian de Souza
    Department of Immunology and Molecular Pathology, Royal Free and University College London Medical School, London W1T 4JF, United Kingdom
    Infect Immun 70:5045-51. 2002
    b>Glycosylphosphatidylinositols (GPIs), the anchor molecules of some membrane proteins of Plasmodium species, have been implicated in the induction of immunopathology during malaria infections...
  42. ncbi Anchorless prion protein results in infectious amyloid disease without clinical scrapie
    Bruce Chesebro
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    Science 308:1435-9. 2005
    ..In contrast, combined expression of anchorless and wild-type PrP produced accelerated clinical scrapie. Thus, the PrP GPI anchor may play a role in the pathogenesis of prion diseases...
  43. ncbi Glycosylphosphatidylinositols in malaria pathogenesis and immunity: potential for therapeutic inhibition and vaccination
    C S Boutlis
    International Health Program, Infectious Diseases Division, Menzies School of Health Research, P O Box 41096, 0811 Casuarina, NT, Australia
    Curr Top Microbiol Immunol 297:145-85. 2005
    b>Glycosylphosphatidylinositols (GPIs) are found in the outer cell membranes of all eukaryotes. GPIs anchor a diverse range of proteins to the surface of Plasmodium falciparum, but may also exist free of protein attachment...
  44. ncbi Deletion of GPI7, a yeast gene required for addition of a side chain to the glycosylphosphatidylinositol (GPI) core structure, affects GPI protein transport, remodeling, and cell wall integrity
    A Benachour
    Institute of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    J Biol Chem 274:15251-61. 1999
    ..Gpi7p contains significant homology with phosphodiesterases suggesting that Gpi7p itself is the transferase adding a side chain to the alpha1,6-linked mannose of the GPI core structure...
  45. pmc Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins
    K Ohishi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 11:1523-33. 2000
    ..Moreover, Gaa1p and Gpi8p are associated with each other. Therefore, Gaa1p and Gpi8p constitute a GPI transamidase and cooperate in generating a carbonyl intermediate, a prerequisite for GPI attachment...
  46. ncbi Glycosylphosphatidylinositol (GPI)-anchored proteins
    Hiroh Ikezawa
    Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan
    Biol Pharm Bull 25:409-17. 2002
    ..GPI-anchored proteins are assumed to be transported from Golgi to the plasma membrane in the form of "lipid rafts", and expressed as the clusters in the cell surface...
  47. ncbi GPI-anchored proteins are delivered to recycling endosomes via a distinct cdc42-regulated, clathrin-independent pinocytic pathway
    Shefali Sabharanjak
    National Centre for Biological Sciences, UAS GKVK Campus, Bellary Road, 560 065, Bangalore, India
    Dev Cell 2:411-23. 2002
    ..These results describe a distinct constitutive pinocytic pathway, specifically regulated by cdc42...
  48. ncbi Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages
    Qing Yuan Yin
    Laboratory for Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Biol Chem 280:20894-901. 2005
    ..Therefore, it will probably be equally effective in many other fungi...
  49. ncbi Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis
    T Kinoshita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, 565 0871, Osaka, Japan
    Curr Opin Chem Biol 4:632-8. 2000
    ..These studies have revealed the common and also different characteristics of glycosylphosphatidyl-inositol biosynthesis enzymes in different organisms, leading to the development of species-specific inhibitors of the pathway...
  50. pmc Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    EMBO J 19:4402-11. 2000
    b>Glycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors...
  51. pmc Protein tyrosine phosphatase alpha (PTPalpha) and contactin form a novel neuronal receptor complex linked to the intracellular tyrosine kinase fyn
    L Zeng
    Institute for Molecular and Cell Biology, Singapore 117609, Republic of Singapore
    J Cell Biol 147:707-14. 1999
    ....
  52. pmc Specific inhibition of GPI-anchored protein function by homing and self-association of specific GPI anchors
    Thomas B Nicholson
    McGill Cancer Centre and Biochemistry Department, McGill University, Montreal, Quebec, Canada H3G 1Y6
    J Cell Biol 175:647-59. 2006
    ..This work clarifies how the GPI anchor can determine protein function, while offering a novel method for its modulation...
  53. pmc Neurodegeneration induced by clustering of sialylated glycosylphosphatidylinositols of prion proteins
    Clive Bate
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, United Kingdom
    J Biol Chem 287:7935-44. 2012
    ....
  54. pmc Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis
    C Sutterlin
    Biozentrum of the University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Biochem J 332:153-9. 1998
    ..Overexpression of GPI10 gives partial resistance to the GPI-synthesis inhibitor YW3548, suggesting that this gene product may affect the target of the inhibitor...
  55. pmc Spontaneous generation of anchorless prions in transgenic mice
    Jan Stöhr
    Institute for Neurodegenerative Diseases, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 108:21223-8. 2011
    ..Remarkably, disease from ill Tg(PrP,ΔGPI) mice transmitted to mice expressing wild-type PrP(C), indicating the spontaneous generation of prions...
  56. ncbi A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor
    R D Klein
    Department of Neuroscience, Genentech, Inc, South San Francisco, California 94080, USA
    Nature 387:717-21. 1997
    ....
  57. ncbi Probing Trypanosoma brucei glycosylphosphatidylinositol biosynthesis using novel precursor-analogues
    Michael D Urbaniak
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Chem Biol Drug Des 72:127-32. 2008
    ..The use of alternative groups, such as sterols, in place of the natural diacylglycerol lipid may enable the production of more drug-like, substrate-based inhibitors...
  58. pmc Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells
    Daniel J Barr
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794 5215, USA
    J Cell Sci 121:3155-66. 2008
    ..Further characterization of this novel clathrin-, caveolae- and Rho-family-independent endocytic pathway might reveal new strategies for the downregulation of ErbB2 in breast cancer...
  59. ncbi Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER
    Andrew K Sobering
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205, USA
    Cell 117:637-48. 2004
    ..We propose that diminished GPI-anchor protein production contributes to hyperactive Ras phenotypes...
  60. pmc The role of the prion protein membrane anchor in prion infection
    Suzette A Priola
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA
    Prion 3:134-8. 2009
    ..In combination with previously published data, our results suggest that GPI anchored PrP is important in the amplification and spread of prion infectivity from cell to cell...
  61. pmc The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    EMBO J 17:877-85. 1998
    ..These results suggest that GPI-GnT recognizes the fatty acyl chains of PI. The unusually complex organization of GPI-GnT may be relevant to selective usage of PI and/or regulation...
  62. pmc Synthesis of potential metal-binding group compounds to examine the zinc dependency of the GPI de-N-acetylase metalloenzyme in Trypanosoma brucei
    Nuha Z Abdelwahab
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, The University of Dundee, DD1 5EH Dundee, Scotland, United Kingdom
    Carbohydr Res 346:708-14. 2011
    ..The N-ureido thioglucoside was also synthesised and added to the ZBG library because a previous N-ureido analogue, synthesised by us, had inhibitory activity against the aforementioned de-N-acetylase, presumably via the N-ureido motif...
  63. ncbi A homologue of Saccharomyces cerevisiae Dpm1p is not sufficient for synthesis of dolichol-phosphate-mannose in mammalian cells
    S Tomita
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    J Biol Chem 273:9249-54. 1998
    ..Therefore, in contrast to yeast, mammalian cells require hDPM1/mDPM1 protein and a product of another gene that is defective in Lec15 mutant cells for synthesis of Dol-P-Man...
  64. pmc Identification of a species-specific inhibitor of glycosylphosphatidylinositol synthesis
    C Sutterlin
    Biozentrum of the University of Basel, Switzerland
    EMBO J 16:6374-83. 1997
    ..These results suggest that despite the conserved core structure, the GPI biosynthetic machinery may be different enough between mammalian and protozoa to represent a target for anti-protozoan chemotherapy...
  65. ncbi Specificities of enzymes of glycosylphosphatidylinositol biosynthesis in Trypanosoma brucei and HeLa cells
    Terry K Smith
    Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:37147-53. 2002
    ..These findings should have a bearing on the design of future generations of specific inhibitors of the trypanosomal glycosylphosphatidylinositol biosynthetic pathway...
  66. ncbi Probing enzymes late in the trypanosomal glycosylphosphatidylinositol biosynthetic pathway with synthetic glycosylphosphatidylinositol analogues
    Michael D Urbaniak
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    ACS Chem Biol 3:625-34. 2008
    ....
  67. pmc Yeast Gpi8p is essential for GPI anchor attachment onto proteins
    M Benghezal
    Institute of Biochemistry, University of Fribourg, Perolles, Switzerland
    EMBO J 15:6575-83. 1996
    ..Humans have a gene which is highly homologous to GPI8 and can functionally replace it...
  68. pmc Intracellular retention of glycosylphosphatidyl inositol-linked proteins in caveolin-deficient cells
    Federica Sotgia
    Department of Molecular Pharmacology, The Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:3905-26. 2002
    ..In further support of these findings, GPI-linked proteins were also retained intracellularly in tissue samples derived from Cav-1 null mice (i.e., lung endothelial and renal epithelial cells) and Cav-3 null mice (skeletal muscle fibers)...
  69. pmc Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins
    D Hamburger
    Biozentrum of the University of Basel, Switzerland
    J Cell Biol 129:629-39. 1995
    Anchoring of proteins to membranes by glycosylphosphatidylinositols (GPIs) is ubiquitous among all eukaryotes and heavily used by parasitic protozoa. GPI is synthesized and transferred en bloc to form GPI-anchored proteins...
  70. pmc Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins
    Yeongjin Hong
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Japan
    Mol Biol Cell 14:1780-9. 2003
    ..Taken together, PIG-U and the yeast orthologue Cdc91p are the fifth component of GPI transamidase that may be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI...
  71. ncbi Further probing of the substrate specificities and inhibition of enzymes involved at an early stage of glycosylphosphatidylinositol (GPI) biosynthesis
    Arthur Crossman
    School of Life Sciences Chemistry, Carnelley Building, University of Dundee, Dundee DD1 4HN, UK
    Carbohydr Res 337:2049-59. 2002
    ..A brief summary of the biological evaluation of the various analogues is provided...
  72. ncbi Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene
    S D Leidich
    Department of Biochemistry, University of Illinois at Urbana Champaign 61801, USA
    J Biol Chem 270:13029-35. 1995
    ....
  73. ncbi Surface antigens of Leishmania mexicana amastigotes: characterization of glycoinositol phospholipids and a macrophage-derived glycosphingolipid
    G Winter
    Max Planck Institut fur Biologie, Abteilung Membranbiochemie, Tubingen, Federal Republic of Germany
    J Cell Sci 107:2471-82. 1994
    ..In contrast to EPiM3, there is strong evidence that this glycosphingolipid is not synthesized by amastigotes but by macrophages in the lesion. This suggests a mechanism of lipid transfer from the macrophage to the parasite...
  74. ncbi Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat
    Daishiro Miura
    Division of Genetic and Reproductive Toxicology, US Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas 72079, USA
    Environ Mol Mutagen 49:614-21. 2008
    ..Although RBCs may be useful for a minimally invasive assay, T-cells are a promising tissue for both detecting GPI-deficient cells and confirming that Pig-A gene mutation is the cause of the phenotype...
  75. ncbi Post-translational GPI lipid anchor modification of proteins in kingdoms of life: analysis of protein sequence data from complete genomes
    B Eisenhaber
    Max Delbruck Centrum fur Molekulare Medizin, Robert Rossle Strasse 10, D 13122 Berlin Buch, Germany
    Protein Eng 14:17-25. 2001
    ..We present a refined model of the transamidase complex...
  76. ncbi N-glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells
    Susan Pang
    School of Biochemistry and Microbiology, University of Leeds, LS2 9JT, UK
    J Cell Sci 117:5079-86. 2004
    ..These data indicate that it is the N-glycans, not the association of the GPI anchor with lipid rafts, which determine apical targeting of an endogenously N-glycosylated, GPI-anchored protein in polarised epithelial cells...
  77. ncbi CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes
    S Ilangumaran
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
    Blood 91:3901-8. 1998
    ..Our results suggest that cellular stimulation via CD44 may proceed through the signaling machinery of glycosphingolipid-enriched plasma membrane microdomains and, hence, depend on the functional integrity of such domains...
  78. pmc Residues surrounding the glycosylphosphatidylinositol anchor attachment site of PrP modulate prion infection: insight from the resistance of rabbits to prion disease
    Rebecca M Nisbet
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria 3010, Australia
    J Virol 84:6678-86. 2010
    ..However, these residues did inhibit PrP(Sc) formation, suggesting that these rabbit-specific residues interfere with a C-terminal PrP(Sc) interaction site...
  79. ncbi CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae
    Vikram Ghugtyal
    University of Fribourg, Department of Medicine, Ch Du Musée 5, CH 1700 Fribourg, Switzerland
    Mol Microbiol 65:1493-502. 2007
    After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by ..
  80. ncbi Paroxysmal nocturnal haemoglobinuria: a replacement of haematopoietic tissue?
    H Schrezenmeier
    Free University of Berlin, University Hospital Benjamin Franklin, Medical Clinic III, Berlin, Germany
    Acta Haematol 103:41-8. 2000
    ..Thus, in at least some patients GPI-AP-deficient cells might pre-exist at a very low percentage and replace haematopoiesis after an insult to the normal cells...
  81. ncbi Structural remodeling of GPI anchors during biosynthesis and after attachment to proteins
    Morihisa Fujita
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    FEBS Lett 584:1670-7. 2010
    ..Here, we describe the structural remodeling of GPI anchors and genes required for the processes in mammals, yeast, and trypanosomes...
  82. pmc Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides
    Mariko Umemura
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki 305 8566, Japan
    Mol Biol Cell 18:4304-16. 2007
    ..Our results also indicate that mouse FRAG1 and C130090K23, which are homologous to Cwh43-N and -C, respectively, share these activities...
  83. ncbi The N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme
    Michael D Urbaniak
    Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, The University of Dundee, Scotland, UK
    J Biol Chem 280:22831-8. 2005
    ..The characterization of GlcNAc-PI de-N-acetylase as a zinc metalloenzyme will facilitate the rational design of anti-protozoan parasite drugs...
  84. pmc The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi
    F Schimmöller
    Biozentrum, University of Basel, Switzerland
    EMBO J 14:1329-39. 1995
    ..We propose that Emp24p is involved in the sorting and/or concentration of a subset of secretory proteins into ER-derived transport vesicles...
  85. ncbi Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 N-acetylglucosaminyl transferase in Trypanosoma brucei
    Luis Izquierdo
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Mol Microbiol 71:478-91. 2009
    ..However, changes in the lectin binding of other glycoproteins suggest that TbGT8 influences the processing of the poly N-acetyllactosamine-containing asparagine-linked glycans of this life cycle stage...
  86. pmc PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor
    Yoshiko Murakami
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 14:4285-95. 2003
    ..The inositol acylation is not essential for the subsequent mannosylation, indicating that glucosaminyl phosphatidylinositol can flip from the cytoplasmic side to the luminal side of the endoplasmic reticulum...
  87. pmc Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts
    D H Nguyen
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 74:3264-72. 2000
    ....
  88. pmc Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies
    Valeria R Caiolfa
    Department of Molecular Biology and Functional Genomics, Unit of Molecular Neuroscience, San Raffaele Scientific Institute, 20132 Milano, Italy
    J Cell Biol 179:1067-82. 2007
    ..These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents...
  89. pmc Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors
    T K Smith
    Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    EMBO J 20:3322-32. 2001
    ..These and previous results were used to design two potent (IC50 = 8 nM) parasite-specific suicide substrate inhibitors. These are potential lead compounds for the development of anti-protozoan parasite drugs...
  90. ncbi Rafts, anchors and viruses--a role for glycosylphosphatidylinositol anchored proteins in the modification of enveloped viruses and viral vectors
    Christoph Metzner
    Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Veterinarplatz 1, 1210 Vienna, Austria
    Virology 382:125-31. 2008
    ..In this review we will look at the link between lipid rafts, GPI-anchored proteins and retroviral particles and how these relationships can be exploited for the modification of enveloped viruses...
  91. pmc Antibodies to Plasmodium falciparum glycosylphosphatidylinositols: inverse association with tolerance of parasitemia in Papua New Guinean children and adults
    Craig S Boutlis
    Department of Tropical Medicine and International Health, Menzies School of Health Research, Casuarina, Australia
    Infect Immun 70:5052-7. 2002
    ....
  92. ncbi Glycosylphosphatidylinositol-anchored surface molecules of Trypanosoma congolense insect forms are developmentally regulated in the tsetse fly
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, 3012, Bern, Switzerland
    Mol Biochem Parasitol 119:7-16. 2002
    ..Unexpectedly, 14 days post-infection, procyclic forms frequently are negative for both PRS and GARP, suggesting that they might be expressing another stage-specific surface antigen at this point in the life cycle...
  93. ncbi GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF
    S Jing
    Department of Immunology, Amgen, Inc, Thousand Oaks, California 91320, USA
    Cell 85:1113-24. 1996
    ..A model for the stepwise formation of a GDNF signal-transducing complex including GDNF, GDNFR-alpha, and the Ret PTK is proposed...
  94. ncbi Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls
    Ada Ching
    DuPont Crop Genetics Research, DuPont Experimental Station Building E353, Route 141 and Henry Clay Road, Wilmington, DE 19880 0353, USA
    Planta 224:1174-84. 2006
    ..Our results demonstrate that mutations in the Bk2 gene affect stalk strength in maize by interfering with the deposition of cellulose in the secondary cell wall in fiber cells...
  95. pmc Fatty acid remodeling of GPI-anchored proteins is required for their raft association
    Yusuke Maeda
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Mol Biol Cell 18:1497-506. 2007
    ..We also show that the remodeling requires the preceding PGAP1-mediated deacylation from inositol of GPI-APs in the endoplasmic reticulum...
  96. pmc The transmembrane protein CBP plays a role in transiently anchoring small clusters of Thy-1, a GPI-anchored protein, to the cytoskeleton
    Yun Chen
    Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7090, USA
    J Cell Sci 122:3966-72. 2009
    ..Finally, we speculate on the role of phosphorylation in the regulation of transient anchorage...
  97. ncbi In vivo mutation assay based on the endogenous Pig-a locus
    Steven M Bryce
    Litron Laboratories, 200 Canal View Blvd, Rochester, New York 14620, USA
    Environ Mol Mutagen 49:256-64. 2008
    ..These data support the hypothesis that an efficient in vivo mutation assay can be developed around flow cytometric enumeration of erythrocytes and/or RETs that exhibit aberrant GPI-anchored protein expression...
  98. ncbi Eimeria tenella sporozoites and merozoites differentially express glycosylphosphatidylinositol-anchored variant surface proteins
    Enrique Tabarés
    Division of Molecular Biology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK
    Mol Biochem Parasitol 135:123-32. 2004
    ..The biological role(s) of EtSAGs and the effect(s) that expression of a complex repertoire of variant surface antigens by the second generation merozoite has on host adapted immunity are unknown...
  99. pmc The role of glycophosphatidylinositol anchor in the amplification of the scrapie isoform of prion protein in vitro
    Jae Il Kim
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106, USA
    FEBS Lett 583:3671-5. 2009
    ..These findings shed a new light on prion protein conversion process and have important implications for the efforts to generate synthetic prions for structural and biophysical studies...
  100. ncbi Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis
    Eduardo J M Nascimento
    Centro de Pesquisas Ageu Magalhães, FIOCRUZ, Cidade Universitaria, Recife, PE, Brasil
    Mem Inst Oswaldo Cruz 102:21-7. 2007
    ..The experiments in vivo showed that under protamine sulphate effect, the scope of protection remained unchanged, but a modulation in antibody production (IgG1= IgG2a) was observed...
  101. ncbi Properties of exogenously added GPI-anchored proteins following their incorporation into cells
    D R Premkumar
    The Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Cell Biochem 82:234-45. 2001
    ..These results have implications for protein engineering of cells in general, and in particular, for cells such as modified tumor cell immunogens administered to patients for therapeutic purposes...

Research Grants63

  1. Impacts of surface receptors [Toll like receptor (TLRs) and Fc gamma receptors (F
    Collins Ouma; Fiscal Year: 2012
    ..This information will be important since the primary goal of our genetic-based investigations is to identify susceptible groups for targeted therapeutic interventions. ..
  2. CD-73 adenosine signaling in the central nervous system in disease and health
    Margaret S Bynoe; Fiscal Year: 2012
    ..Such therapies could be used to stop the progress of, or even possibly reverse the damage caused by MS. ..
  3. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..Goals of this pilot study are to test a new hypothesis for how these enzymes work, and to determine how disease-causing mutations affect enzyme function. ..
  4. The Regulation of Inflammatory Responses by CD73
    Linda F Thompson; Fiscal Year: 2012
    ..The results of the proposed studies may lead to the development of new therapies for a variety of serious medical conditions that affect millions of Americans including sepsis, inflammation, and autoimmunity. ..
  5. Spatiotemporal Regulation of Membrane Raft Trafficking in Virus Activated Cells
    Tione Buranda; Fiscal Year: 2012
    ..The long-term result of this work could be used to determine route of entry for anti-viral treatment. ..
  6. Synthetic Oligosaccharides for Probing Infection and Innate Immunity
    Geert Jan Boons; Fiscal Year: 2013
    ..The new methodology will be employed to probe the ligand requirements of var2CSA and several chemokines. ..
  7. Apolipoprotein A-V: A Functional Proteomics Study
    ROBERT O apos MARA RYAN; Fiscal Year: 2010
    ..Improvements in diagnosis, prevention and/or treatment could have a major impact on the large population of Americans at risk for heart disease, obesity and Type 2 diabetes. ..
  8. The Role of Lipid Rafts in Vpu Function
    EDWARD BRICE STEPHENS; Fiscal Year: 2011
    ..The information gained from these studies may result in novel antiviral strategies against HIV-1. ..
  9. Innate Immune Activation in Malaria
    Douglas T Golenbock; Fiscal Year: 2013
    ..The purpose of this grant is to gain a better understanding of why malaria causes disease in the hopes that better therapies can be devised, including an effective vaccine that might prevent malaria. ..
  10. Glycolipid-anchored cytokines as breast cancer membrane vaccine adjuvants
    Periasamy Selvaraj; Fiscal Year: 2013
    ..abstract_text> ..
  11. Structural Analysis of the GPI Transamidase Complex
    Anant K Menon; Fiscal Year: 2012
    ....
  12. Secretion, protein targeting, and lipid metabolism in African Trypanosomes
    James D Bangs; Fiscal Year: 2013
    ..These studies will broaden our knowledge of basic cell biological processes in all eukaryotes, and will lay the groundwork for targeting the sphingolipid pathway for novel drug development. ..
  13. BIOSYNTHESIS OF MEMBRANE PROTEIN GLYCOLIPID ANCHORS
    Anant K Menon; Fiscal Year: 2010
    ....
  14. New Molecules in Triglyceride Metabolism and Adipogenesis
    Stephen G Young; Fiscal Year: 2012
    ..An Administration Core (Core B) will support the personnel of each project, organize advisory board meetings, and ensure compliance with institutional and NIH guidelines. ..
  15. Development of HTS Assays for Inhibitors of Viral-Cell Interactions
    Tione Buranda; Fiscal Year: 2010
    ..Successful completion of this project will lead to the development of therapeutic intervention-probes for hantavirus infection and other pathogens that rely on these receptors for infection. ..
  16. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2012
    ..provided by applicant): Many surface proteins and glycoproteins are anchored to cell membranes by glycosylphosphatidylinositols (GPIs), and GPI-anchored proteins and glycoproteins play an important role in various biological ..
  17. Epididymal PMCA4 Expression Functional Impact and Mechanism of Sperm Uptake
    PATRICIA ANASTASIA DELEON; Fiscal Year: 2013
    ....
  18. TFPI ALPHA AND TFPI BETA
    George J Broze; Fiscal Year: 2013
    ....
  19. NK Cell Biology
    Lewis L Lanier; Fiscal Year: 2013
    ..abstract_text> ..
  20. Tumor antigen-decorated virus-like particles as a therapeutic cancer vaccine
    Jaina Patel; Fiscal Year: 2013
    ..The knowledge obtained from these studies will form a foundation to develop VLP-based efficacious therapeutic cancer vaccines to treat existing tumors or lead to the prevention of metastatic growth in humans. ..
  21. Validation of a Rodent Mutagenicity Assay
    Stephen D Dertinger; Fiscal Year: 2012
    ..Additional work will be directed at developing mutant cell scoring methods that are compatible with other tissues. ..
  22. Regulation of Mechano-Induced TGFb1 Activation and Myofibroblast Differentiation
    Yong Zhou; Fiscal Year: 2010
    ..The proposed studies will explore the molecular mechanisms involved in the pathogenesis of lung fibrosis. Our goal is to develop novel therapeutic interventions for IPF treatment. ..
  23. Signaling GPI-phosphlipase C of a Trypanosome
    Kojo Mensa Wilmot; Fiscal Year: 2010
    ..brucei. Trypanosomes cause diseases that affect millions of people world-wide. Work described in this proposal may lead to discovery of new signaling pathways that may be targeted for treatment of human African trypanosomiasis. ..
  24. REGULATION OF PROTEIN N-GLYCOSYLATION IN THE CNS
    CHARLES WAECHTER; Fiscal Year: 2009
    ....
  25. Functional roles of stimulated recycling endosome exocytosis in mast cells
    Joshua D Wilson; Fiscal Year: 2012
    ..The proposed studies will advance our understanding of the functional roles of recycling endosomes in mast cells and, in general, of stimulated membrane trafficking in cell biology and immunology. ..
  26. Vitamin D Regulation of CD14 in Mammary Epithelial Cells
    KATRINA MARIE SIMMONS; Fiscal Year: 2013
    ..Overall this work will enhance our understanding of the immunological functions of vitamin D in epithelial tissues. ..
  27. Evaluation of a synthetic anti-GPI vaccine candidate in severe anemia models of m
    A Stewart Campbell; Fiscal Year: 2010
    ..Severe anemia is a major complication due to malaria infection, especially in young children. This proposal aims to provide key proof-of-concept data that the anti-toxin vaccine approach may protect against severe malaria anemia. ..
  28. Tools to Study Mammalian Mutagenesis
    Stephen D Dertinger; Fiscal Year: 2013
    ..The bridging capabilities and high throughput nature of the proposed mutation assessment tools will contribute significantly to important areas of research that include academic, regulatory, and industrial settings. ..
  29. In Search of Mannolipid Intermediate Flippases
    Charles J Waechter; Fiscal Year: 2013
    ..The discovery of genes encoding membrane proteins functioning as flippases in these assembly processes will be valuable in the diagnosis of potential genetic errors referred to as Congenital Disorders in Glycosylation (CDGs). ..
  30. Intercellular transfer of prion in prion disease
    Man Sun Sy; Fiscal Year: 2006
    ..New insights into this area will also lead to designs of more rational and effective treatment for prion diseases. [unreadable] [unreadable]..
  31. INACTIVATION OF THE PIGA GENE IN HEMATOPOIETIC STEM CELL
    Monica Bessler; Fiscal Year: 2002
    ..The findings could provide the scientific foundation of new therapeutic modalities for PNH and other bone marrow failure syndromes. ..
  32. ROLE OF THE PNH PHENOTYPE IN LEUKEMIC TRANSFORMATION
    Monica Bessler; Fiscal Year: 2005
    ..The availability of a mouse model for PNH will provide us with a powerful tool to test new therapeutic agents for the treatment of PNH, PNH/MDS, PNH,AML and possibly other clonal blood disorders. ..
  33. IGA FC RECEPTORS
    JULIA PHILLIPS QUAGLIATA; Fiscal Year: 1993
    ..5) To establish whether the receptor plays a physiological role in the function of the cells...
  34. Enhancing axon regeneration by multi-inhibitor blocking
    RONALD SCHNAAR; Fiscal Year: 2005
    ..The resulting data may provide insight on the relative contributions of the different ARI's in an in vivo model, and may support evaluation of new approaches to enhance axon regeneration after CNS injury. ..
  35. BIOGENESIS OF THE CHLAMYDIA TRACHOMATIS VACUOLE
    Joanne Engel; Fiscal Year: 2004
    ..trachomatis vacuole. This will help to further define the pathway involved. These studies may lead to the development of new anti-chlamydial drug therapies and further our understanding of lipid trafficking in eukaryotic cells. ..
  36. LIGAND BONDING FORCE AND CELL ADHESION BY CD16 ISOFORMS
    Cheng Zhu; Fiscal Year: 1999
    ..The proposed research will contribute to our understanding as to how the membrane anchor of Fc receptors affects their properties, their interactions with ligands, and the ability of such interactions to induce effector functions. ..
  37. INTESTINAL PROTEIN METABOLISM AND FUNCTION
    David Alpers; Fiscal Year: 2000
    ....
  38. EXPRESSION OF GLYCOLIPID ANCHORED PRIONS IN TRYPANOSOMES
    GEORGE CROSS; Fiscal Year: 2001
    ..This proposal will focus on producing different forms of PrP in T. brucei. ..
  39. Protein Signatures of Normal versus Paroxysmal Nocturnal Hemoglobinuria Platelets
    Monica Bessler; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  40. GPI PHOSPHOLIPASE C OF T BRUCEI
    Kojo Mensa Wilmot; Fiscal Year: 2001
    ..b>Glycosylphosphatidylinositols (GPIs) are the anchors by which VSGs of varying polypeptide sequences are attached to the parasite ..
  41. Specificity and Structure: The Kex 2 and Furin Proteases
    Robert Fuller; Fiscal Year: 2005
    ..2. To elucidate the biochemistry and physiology of processing of yeast cell wall proteins by Kex2p and GPI-anchored aspartyl proteases. 3. To achieve Kex2p and furin crystallization to advance determination of their structures. ..
  42. SURFACE PROTEINS OF TOXOPLASMA GONDII
    Gary Ward; Fiscal Year: 2002
    ..Immunochemical, cell biological, and molecular genetic approaches will then be used to (4) Determine the biological function of new parasite surface proteins identified in the course of accomplishing Specific Aims 1-3. ..
  43. MOLECULAR GENETICS OF YEAST SEXUAL AGGLUTININS
    JANET KURJAN; Fiscal Year: 1999
    ..Because cell wall structure in other fungi is similar to yeast, investigation of the mechanism of cell wall biogenesis in S. cerevisiae may lead to the development of therapeutic agents against medically important fungi. ..
  44. MOLECULAR BASIS OF THE PED GENE PHENOTYPE
    Carol Warner; Fiscal Year: 1999
    ....
  45. INOSITOL-ACYLATED GPI ANCHORS: BIOSYNTHESIS AND FUNCTION
    MARTIN LOW; Fiscal Year: 1999
    ..The results of these studies will therefore have significant implications for understanding the molecular basis of human disease. ..
  46. Versatile Mutation Assay Based on the Pig-A Locus
    Stephen Dertinger; Fiscal Year: 2009
    ..This research project will refine and validate a powerful new method for detecting mutagenic agents, thereby enhancing the nation's ability to effectively reduce exposure to these toxic compounds. ..
  47. PROTEIN PAINTING OF PREIMPLANTATION EMBRYOS
    Carol Warner; Fiscal Year: 2003
    ..Protein painting may ultimately be applied to create better conditions for modulating preimplantation embryonic growth or death, leading to enhanced pregnancy outcome or a new contraceptive technology. ..
  48. Zinc metalloprotease families in Trypansoma brucei
    JOHN DONELSON; Fiscal Year: 2009
    ....
  49. TFPI ALPHA AND TFPI BETA
    George Broze; Fiscal Year: 2009
    ....
  50. REGULATION AND FUNCTION OF GPI PHOSPHOLIPASE D
    Mark Deeg; Fiscal Year: 1999
    ..Thus, understanding apolipoprotein AI regulation of GPI-PLD has broad implications in a number of vital biological functions with potential alterations in diabetes mellitus and dyslipidemias. ..
  51. THYMIC CLASS I MHC CELL BIOLOGY AND T CELL DEVELOPMENT
    MARTHA ZUNIGA; Fiscal Year: 2000
    ..These studies have implications for malfunctions of the immune system such as autoimmunity and immunodeficiency diseases. ..
  52. SEPARATE ACTIVE DOMAINS OF ANGIOTENSIN CONVERTING ENZYME
    ERVIN ERDOS; Fiscal Year: 2000
    ..Ultimately, the experiments should lead to a better understanding of the structure and function of ACE and the therapeutic applications of the inhibitors. ..
  53. RIN1, A NOVEL RAS-INHIBITORY PROTEIN IN YEAST
    David Levin; Fiscal Year: 2006
    ..These experiments will help to test the applicant's model of Rin1 function as an inhibitor of Ras signaling in the ER. [unreadable] [unreadable]..
  54. DEVELOPMENT AND CHARACTERIZATION OF CD14 DEFICIENT MICE
    Kathryn Moore; Fiscal Year: 2005
    ..This work is intended to broaden the applicability of CD14 deficient mice to research involving acute and chronic inflammatory disease and to make a critical animal resource available to the investigative community at large. ..
  55. Biomarkers and Therapeutic Targets in Pancreatic Cancer
    Edward Whang; Fiscal Year: 2009
    ..By characterizing CEACAM6 and RRM2 signaling pathways, we hope to identify: 1) novel therapeutic targets, 2) new prognostic factors, and 3) early detection strategies for pancreatic cancer. ..
  56. Inositol Isomer Transport Kinetics and Utilization
    PHILLIP RYALS; Fiscal Year: 2002
    ....
  57. GLYCOSYLPHOSPHATIDYLINOSITOL (GPI) ANCHORED ANTITHROMBIN
    HERBERT WHINNA; Fiscal Year: 2003
    ..abstract_text> ..
  58. STUDIES ON THE GENERATION OF INSULIN MEDIATORS
    Guillermo Romero; Fiscal Year: 1993
    ....
  59. OLIGODENDROCYTE/MYELIN GLYCOPROTEIN AND THE P
    Daniel Mikol; Fiscal Year: 2001
    ..Compare the ability of polyclonal anti-OMgp antibodies to induce a myelinating phenotype and/or to activate tyrosine kinase pathways in native vs. mutant OMgp transfected cells. ..
  60. ABNORMAL PIG A EXPRESSION IN GPI DEFICIENT BLOOD CELLS
    Russell Ware; Fiscal Year: 1999
    ..The effects of abnormal Piga expression on lymphocyte growth will also be studied by transfection of Piga mutants and inhibition of normal Piga expression. ..
  61. PROTEOGLYCANS AND BRAIN DEVELOPMENT
    ARTHUR LANDER; Fiscal Year: 2004
    ..These studies should provide insights into how an important and poorly understood class of molecules regulates basic developmental processes, particularly in the nervous system. ..
  62. ACINAR-DUCT CELL COUPLING IN PANCREATIC FUNCTION
    Steven Freedman; Fiscal Year: 2006
    ..Results from this project will be important in understanding the pathophysiology of CF and the development of potential therapies for diseases related to CF gene mutations. ..
  63. Regulation of spinal cord development and the Wnt signaling pathway by Oto
    ROEBEN MUNJI; Fiscal Year: 2009
    ..This proposal will allow new understanding of the molecular and developmental events causing these important syndromes. ..